Unmanned aerial systems (UAS) carrying commercially sold multispectral sensors equipped with a sunshine sensor, such as Parrot Sequoia, enable mapping of vegetation at high spatial resolution with a large degree of flexibility in planning data collection. It is, however, a challenge to perform radiometric correction of the images to create reflectance maps (orthomosaics with surface reflectance) and to compute vegetation indices with sufficient accuracy to enable comparisons between data collected at different times and locations. Studies have compared different radiometric correction methods applied to the Sequoia camera, but there is no consensus about a standard method that provides consistent results for all spectral bands and for different flight conditions. In this study, we perform experiments to assess the accuracy of the Parrot Sequoia camera and sunshine sensor to get an indication if the quality of the data collected is sufficient to create accurate reflectance maps. In addition, we study if there is an influence of the atmosphere on the images and suggest a workflow to collect and process images to create a reflectance map. The main findings are that the sensitivity of the camera is influenced by camera temperature and that the atmosphere influences the images. Hence, we suggest letting the camera warm up before image collection and capturing images of reflectance calibration panels at an elevation close to the maximum flying height to compensate for influence from the atmosphere. The results also show that there is a strong influence of the orientation of the sunshine sensor. This introduces noise and limits the use of the raw sunshine sensor data to compensate for differences in light conditions. To handle this noise, we fit smoothing functions to the sunshine sensor data before we perform irradiance normalization of the images. The developed workflow is evaluated against data from a handheld spectroradiometer, giving the highest correlation (R² = 0.99) for the normalized difference vegetation index (NDVI). For the individual wavelength bands, R² was 0.80–0.97 for the red-edge, near-infrared, and red bands.

Modern potato breeding methods following a genomic-led approach provide means for shortening breeding cycles and increasing breeding efficiency across selection cycles. Acquiring genetic data for large breeding populations remains expensive. We present a pipeline to reduce the number of single nucleotide polymorphisms (SNPs) to lower the cost of genotyping. First, we reduced the number of individuals to be genotyped with a high-throughput method according to the multi-trait variation as defined by principal component analysis of phenotypic characteristics. Next, we reduced the number of SNPs by pruning for linkage disequilibrium. By adjusting the square of the correlation coefficient between two adjacent loci, we obtained reduced subsets of SNPs. We subsequently tested these SNP subsets by two methods; (1) a genome-wide association study (GWAS) for marker identification, and (2) genomic selection (GS) to predict genomic estimated breeding values. The results indicate that both GWAS and GS can be done without loss of information after SNP reduction. The pipeline allows for creating custom SNP subsets to cover all variation found in any particular breeding population. Low-throughput genotyping will reduce the genotyping cost associated with large populations, thereby making genomic breeding methods applicable to large potato breeding populations by reducing genotyping costs.