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Dr. Małgorzata Szultka-Młyńska
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Torun, Poland

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0 Chromatography
0 In Vitro
0 In Vivo
0 Mass Spectrometry
0 Metabolomics

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microextraction
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Journal article
Published: 27 August 2021 in Materials
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The aim of this study was to examine the synthesis of novel molecularly imprinted polymer (MIP)-coated polythiophene and poly(3-methylthiophene) solid-phase microextraction fibers using the direct electropolymerization method. Synthesized SPME fibers were characterized with the use of various physicochemical instrumental techniques. MIP-SPME coatings were successfully applied to carry out the selective extraction of selected antibiotic drugs (amoxicillin, cefotaxime, metronidazole) and their metabolites (amoxycilloic acid, amoxicillin diketopiperazine, desacetyl cefotaxime, 3-desacetyl cefotaxime lactone, hydroxymetronidazole). Solid-phase microextraction parameters for the simultaneous determination and identification of target compounds were optimized using the central composite design (CCD), and they accounted for 5–15 min for desorption time, 3–10 for the pH of the desorption solvent, and 30–100 μL for the volume of the desorption solvent. High-performance liquid chromatography and mass spectrometry (MS) detectors such as quadrupole time-of-flight (Q-TOF MS) and triple quadrupole (QqQ MS) were applied to determine and to identify selected antibiotic drugs and their metabolites. The MIP-coated SPME are suitable for the selective extraction of target compounds in biological samples from patients in intensive care units.

ACS Style

Małgorzata Szultka-Młyńska; Daria Janiszewska; Bogusław Buszewski. Molecularly Imprinted Polymers as Solid-Phase Microextraction Fibers for the Isolation of Selected Antibiotics from Human Plasma. Materials 2021, 14, 4886 .

AMA Style

Małgorzata Szultka-Młyńska, Daria Janiszewska, Bogusław Buszewski. Molecularly Imprinted Polymers as Solid-Phase Microextraction Fibers for the Isolation of Selected Antibiotics from Human Plasma. Materials. 2021; 14 (17):4886.

Chicago/Turabian Style

Małgorzata Szultka-Młyńska; Daria Janiszewska; Bogusław Buszewski. 2021. "Molecularly Imprinted Polymers as Solid-Phase Microextraction Fibers for the Isolation of Selected Antibiotics from Human Plasma." Materials 14, no. 17: 4886.

Journal article
Published: 18 August 2021 in Molecules
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The bacterial infection of post-operative wounds is a common health problem. Therefore, it is important to investigate fast and accurate methods of identifying bacteria in clinical samples. The aim of the study was to analyse the use of the MALDI-TOF MS technique to identify microorganism wounds that are difficult to heal. The most common bacteria are Escherichia coli, Staphylococcus spp., and Enterococcus spp. We also demonstrate the effect of culture conditions, such as the used growth medium (solid: Brain Heart Infusion Agar, Mueller Hilton Agar, Glucose Bromocresol Purple Agar, and Vancomycin Resistance Enterococci Agar Base and liquid: Tryptic Soy Broth and BACTEC Lytic/10 Anaerobic/F), the incubation time (4, 6, and 24h), and the method of the preparation of bacterial protein extracts (the standard method based on the Bruker guideline, the Sepsityper method) to identify factors and the quality of the obtained mass spectra. By comparing the protein profiles of bacteria from patients not treated with antibiotics to those treated with antibiotics based on the presence/absence of specific signals and using the UniProt platform, it was possible to predict the probable mechanism of the action of the antibiotic used and the mechanism of drug resistance.

ACS Style

Małgorzata Szultka-Młyńska; Daria Janiszewska; Paweł Pomastowski; Michał Złoch; Wojciech Kupczyk; Bogusław Buszewski. Identification of Bacteria Associated with Post-Operative Wounds of Patients with the Use of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Approach. Molecules 2021, 26, 5007 .

AMA Style

Małgorzata Szultka-Młyńska, Daria Janiszewska, Paweł Pomastowski, Michał Złoch, Wojciech Kupczyk, Bogusław Buszewski. Identification of Bacteria Associated with Post-Operative Wounds of Patients with the Use of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Approach. Molecules. 2021; 26 (16):5007.

Chicago/Turabian Style

Małgorzata Szultka-Młyńska; Daria Janiszewska; Paweł Pomastowski; Michał Złoch; Wojciech Kupczyk; Bogusław Buszewski. 2021. "Identification of Bacteria Associated with Post-Operative Wounds of Patients with the Use of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Approach." Molecules 26, no. 16: 5007.

Journal article
Published: 20 March 2021 in Molecules
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Melanoma is an enormous global health burden, and should be effectively addressed with better therapeutic strategies. Therefore, new therapeutic agents are needed for the management of this disease. The aim of this study was the investigation of cytotoxic activity of some isoquinoline alkaloid standards and extracts obtained from Sanguinaria canadensis—collected before, during, and after flowering—against three different human melanoma cells (A375, G361, SK-MEL-3). The cytotoxicity of these extracts was not previously tested on these melanoma cell lines. Determination of alkaloid contents was performed by HPLC-DAD using Polar RP column and mobile phase containing acetonitrile, water, and 1-butyl-3-methylimidazolium tetrafluoroborate. The cytotoxicity of alkaloid standards was investigated by determination of cell viability and calculation of IC50 values. Significant differences were observed in the alkaloids content and cytotoxic activity of the extracts, depending on the season of collection of the plant material. In the Sanguinaria canadensis extracts high contents of sanguinarine (from 4.8543 to 9.5899 mg/g of dry plant material) and chelerythrine (from 42.7224 to 6.8722 mg/g of dry plant material) were found. For both of these alkaloids, very high cytotoxic activity against the tested cell lines were observed. The IC50 values were in the range of 0.11–0.54 µg/mL for sanguinarine and 0.14 to 0.46 µg/mL for chelerythrine. IC50 values obtained for Sanguinaria canadensis extracts against all tested cell lines were also very low (from 0.88 to 10.96 µg/mL). Cytotoxic activity of alkaloid standards and Sanguinaria canadensis extracts were compared with the cytotoxicity of anticancer drugs—etoposide, cisplatin, and hydroxyurea. In all cases except the one obtained for cisplatin against A375, which was similar to that obtained for Sanguinaria canadensis after flowering against the same cell line, IC50 values obtained for anticancer drugs were higher than the IC50 values obtained for sanguinarine, chelerythrine, and Sanguinaria canadensis extracts. Our results showed that Sanguinaria canadensis extracts and isoquinoline alkaloids, especially sanguinarine and chelerythrine, could be recommended for further in vivo experiments in order to confirm the possibility of their application in the treatment of human melanomas.

ACS Style

Tomasz Tuzimski; Anna Petruczynik; Tomasz Plech; Barbara Kaproń; Anna Makuch-Kocka; Małgorzata Szultka-Młyńska; Justyna Misiurek; Bogusław Buszewski. Determination of Cytotoxic Activity of Sanguinaria canadensis Extracts against Human Melanoma Cells and Comparison of Their Cytotoxicity with Cytotoxicity of Some Anticancer Drugs. Molecules 2021, 26, 1738 .

AMA Style

Tomasz Tuzimski, Anna Petruczynik, Tomasz Plech, Barbara Kaproń, Anna Makuch-Kocka, Małgorzata Szultka-Młyńska, Justyna Misiurek, Bogusław Buszewski. Determination of Cytotoxic Activity of Sanguinaria canadensis Extracts against Human Melanoma Cells and Comparison of Their Cytotoxicity with Cytotoxicity of Some Anticancer Drugs. Molecules. 2021; 26 (6):1738.

Chicago/Turabian Style

Tomasz Tuzimski; Anna Petruczynik; Tomasz Plech; Barbara Kaproń; Anna Makuch-Kocka; Małgorzata Szultka-Młyńska; Justyna Misiurek; Bogusław Buszewski. 2021. "Determination of Cytotoxic Activity of Sanguinaria canadensis Extracts against Human Melanoma Cells and Comparison of Their Cytotoxicity with Cytotoxicity of Some Anticancer Drugs." Molecules 26, no. 6: 1738.

Journal article
Published: 04 February 2021 in Molecules
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Melanoma is a serious form of skin cancer that begins in cells known as melanocytes. While it is less common than the other forms of skin cancer, melanoma is more dangerous because of its ability to spread to other organs more rapidly if it is not treated at an early stage. The number of people diagnosed with melanoma has increased over the last few decades. The most widely used treatments include surgery, chemotherapy, and radiation therapy. The search for new drugs to treat various cancers is one of the most important challenges of modern scientific research. Some isoquinoline alkaloids found in different plant species have strong cytotoxic effects on various cancer cells. We tested the effect of isoquinoline alkaloids and extracts obtained from various parts of Mahonia aquifolium collected in various vegetation seasons on human melanoma cancer cells and our data indicated that investigated extract induced significant reduction in cell viability of Human malignant melanoma cells (A375), human Caucasian malignant melanoma cell line (G361), and human malignant melanoma cell line (SKMEL3 cancer cell lines in a dose- and time-dependent manner. Differences in cytotoxic activity were observed for extracts obtained from various parts of Mahonia aquifolium. Significant differences were also obtained in the alkaloids content and cytotoxic activity of the extracts depending on the season of collection of plant material. Our investigations exhibit that these plant extracts can be recommended for further in vivo experiments in order to confirm the possibility of their use in the treatment of human melanomas.

ACS Style

Tomasz Tuzimski; Anna Petruczynik; Barbara Kaproń; Anna Makuch-Kocka; Małgorzata Szultka-Młyńska; Justyna Misiurek; Grażyna Szymczak; Bogusław Buszewski. Determination of Cytotoxic Activity of Selected Isoquinoline Alkaloids and Plant Extracts Obtained from Various Parts of Mahonia aquifolium Collected in Various Vegetation Seasons. Molecules 2021, 26, 816 .

AMA Style

Tomasz Tuzimski, Anna Petruczynik, Barbara Kaproń, Anna Makuch-Kocka, Małgorzata Szultka-Młyńska, Justyna Misiurek, Grażyna Szymczak, Bogusław Buszewski. Determination of Cytotoxic Activity of Selected Isoquinoline Alkaloids and Plant Extracts Obtained from Various Parts of Mahonia aquifolium Collected in Various Vegetation Seasons. Molecules. 2021; 26 (4):816.

Chicago/Turabian Style

Tomasz Tuzimski; Anna Petruczynik; Barbara Kaproń; Anna Makuch-Kocka; Małgorzata Szultka-Młyńska; Justyna Misiurek; Grażyna Szymczak; Bogusław Buszewski. 2021. "Determination of Cytotoxic Activity of Selected Isoquinoline Alkaloids and Plant Extracts Obtained from Various Parts of Mahonia aquifolium Collected in Various Vegetation Seasons." Molecules 26, no. 4: 816.

Journal article
Published: 01 August 2020 in Future Microbiology
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Aim: The purpose of this study was to create an alternative protocol for the DNA-based identification of salivary microbiota focused on the distinguishing of Streptococcus species. Materials & methods: Salivary bacteria were identified using 16S rDNA sequencing and proteins and lipids profiling using MALDI-TOF/MS as well as FTIR analysis. Results: Most of the isolates belonged to streptococci - mostly the salivarious group indistinguishable by the molecular technique. In turn, MALDI analysis allowed for their fast and reliable classification. Although FTIR spectroscopy demonstrated the correct species classification, the spectra interpretation was time consuming and complicated. Conclusion: MALDI-TOF/MS demonstrated the biggest effectiveness in the identification and discrimination between the salivary streptococci, which could be easily incorporated in the workflow of routine microbiological laboratories.

ACS Style

Michał Złoch; Agnieszka Rodzik; Katarzyna Pauter; Małgorzata Szultka-Młyńska; Agnieszka Rogowska; Wojciech Kupczyk; Paweł Pomastowski; Bogusław Buszewski. Problems with identifying and distinguishing salivary streptococci: a multi-instrumental approach. Future Microbiology 2020, 15, 1157 -1171.

AMA Style

Michał Złoch, Agnieszka Rodzik, Katarzyna Pauter, Małgorzata Szultka-Młyńska, Agnieszka Rogowska, Wojciech Kupczyk, Paweł Pomastowski, Bogusław Buszewski. Problems with identifying and distinguishing salivary streptococci: a multi-instrumental approach. Future Microbiology. 2020; 15 (12):1157-1171.

Chicago/Turabian Style

Michał Złoch; Agnieszka Rodzik; Katarzyna Pauter; Małgorzata Szultka-Młyńska; Agnieszka Rogowska; Wojciech Kupczyk; Paweł Pomastowski; Bogusław Buszewski. 2020. "Problems with identifying and distinguishing salivary streptococci: a multi-instrumental approach." Future Microbiology 15, no. 12: 1157-1171.

Review
Published: 31 May 2020 in Molecules
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Antibiotics were initially natural substances. However, nowadays, they also include synthetic drugs, which show their activity against bacteria, killing or inhibiting their growth and division. Thanks to these properties, many antibiotics have quickly found practical application in the fight against infectious diseases such as tuberculosis, syphilis, gastrointestinal infections, pneumonia, bronchitis, meningitis and septicemia. Antibiotic resistance is currently a detrimental problem; therefore, in addition to the improvement of antibiotic therapy, attention should also be paid to active metabolites in the body, which may play an important role in exacerbating the existing problem. Taking into account the clinical, cognitive and diagnostic purposes of drug monitoring, it is important to select an appropriate analytical method that meets all the requirements. The detection and identification of the microorganism responsible for the infection is also an essential factor in the implementation of appropriate antibiotic therapy. In recent years, clinical microbiology laboratories have experienced revolutionary changes in the way microorganisms are identified. The MALDI-TOF MS technique may be interesting, especially in some areas where a quick analysis is required, as is the case with clinical microbiology. This method is not targeted, which means that no prior knowledge of the infectious agent is required, since identification is based on a database match.

ACS Style

Katarzyna Pauter; Małgorzata Szultka-Młyńska; Bogusław Buszewski. Determination and Identification of Antibiotic Drugs and Bacterial Strains in Biological Samples. Molecules 2020, 25, 2556 .

AMA Style

Katarzyna Pauter, Małgorzata Szultka-Młyńska, Bogusław Buszewski. Determination and Identification of Antibiotic Drugs and Bacterial Strains in Biological Samples. Molecules. 2020; 25 (11):2556.

Chicago/Turabian Style

Katarzyna Pauter; Małgorzata Szultka-Młyńska; Bogusław Buszewski. 2020. "Determination and Identification of Antibiotic Drugs and Bacterial Strains in Biological Samples." Molecules 25, no. 11: 2556.

Journal article
Published: 19 February 2020 in Journal of Pharmaceutical and Biomedical Analysis
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The separation of eleven antibiotics and ten metabolites were studied using high performance liquid chromatography. The C18-PFP octadecyl with integral PFP, C18-AR octadecyl with integral phenyl, C18-HL octadecyl and phenyl phase were used as stationary phases. Mixtures of acetontrile–0.1 % formic acid in water were investigated as mobile phases. The elution order of the target compounds was similar for all four HPLC columns applied. The best separation was obtained using the column with the pentafluorophenylpropyl chain. In addition, in order to optimize the parameters of retention elution for the column and to predict the conditions for the best separation of the active compounds studied biologically the ChromSword software was used. To obtain reliable information of the physicochemical properties and to estimate the relative biological activity of a group of the studied analytes, the QSRR approach was applied. Molecular descriptors were calculated using the HyperChem software. The study was based on multiple linear regression and the results were presented as quantitative structure–retention relationships equations. The QSRR models were determined using 16 molecular descriptors mainly related to the dipole moment (μ), the solvent accessible surface area (SAS), the van der Waals surface area (VWS), the minimum charge (δmin) as well as the polar surface area (PSA). Moreover, structural descriptors of the target compounds were used to describe their chromatographic retention behavior under the optimized HPLC conditions.

ACS Style

Justyna Walczak-Skierska; Małgorzata Szultka-Młyńska; Katarzyna Pauter; Bogusław Buszewski. Study of chromatographic behavior of antibiotic drugs and their metabolites based on quantitative structure-retention relationships with the use of HPLC-DAD. Journal of Pharmaceutical and Biomedical Analysis 2020, 184, 113187 .

AMA Style

Justyna Walczak-Skierska, Małgorzata Szultka-Młyńska, Katarzyna Pauter, Bogusław Buszewski. Study of chromatographic behavior of antibiotic drugs and their metabolites based on quantitative structure-retention relationships with the use of HPLC-DAD. Journal of Pharmaceutical and Biomedical Analysis. 2020; 184 ():113187.

Chicago/Turabian Style

Justyna Walczak-Skierska; Małgorzata Szultka-Młyńska; Katarzyna Pauter; Bogusław Buszewski. 2020. "Study of chromatographic behavior of antibiotic drugs and their metabolites based on quantitative structure-retention relationships with the use of HPLC-DAD." Journal of Pharmaceutical and Biomedical Analysis 184, no. : 113187.

Journal article
Published: 01 December 2019 in Nova Biotechnologica et chimica
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Drug metabolism in liver microsomes was studied in vitro using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Relevant drug was incubated with dog, human and rat liver microsomes (DLMs, HLMs, RLMs) along with NADPH, and the reaction mixture was analyzed by LC-MS/MS to obtain specific metabolic profile. GRACE analytical C18 column, Vision HT (50 × 2 mm, 1.5 μm) was implemented with acetonitrile and water (+ 5 mM ammonium acetate) in a gradient mode as the mobile phase at a flow 0.4 mL.min−1. Different phase I and phase II metabolites were detected and structurally described. The metabolism of the studied drugs occurred via oxidation, hydroxylation and oxidative deamination processes. Conjugates with the glucuronic acid and sulfate were also observed as phase II biotransformation. The central composite design (CCD) showed that factors, such as time incubation, liver microsomal enzymes concentration and NADPH concentration, along with drying gas temperature, nebulizer gas pressure and capillary voltage significantly affected the final response of the method. This study describes the novel information about the chemical structure of the potential metabolites of selected biologically active compounds, which provide vital data for further pharmacokinetic and in vivo metabolism studies.

ACS Style

Małgorzata Szultka-Młyńska; Katarzyna Pauter; Boguslaw Buszewski. Identification of in vitro and in vivo potential metabolites of novel cardiovascular and adrenolytic drugs by liquid chromatography-mass spectrometry with the aid of experimental design. Nova Biotechnologica et chimica 2019, 18, 179 -194.

AMA Style

Małgorzata Szultka-Młyńska, Katarzyna Pauter, Boguslaw Buszewski. Identification of in vitro and in vivo potential metabolites of novel cardiovascular and adrenolytic drugs by liquid chromatography-mass spectrometry with the aid of experimental design. Nova Biotechnologica et chimica. 2019; 18 (2):179-194.

Chicago/Turabian Style

Małgorzata Szultka-Młyńska; Katarzyna Pauter; Boguslaw Buszewski. 2019. "Identification of in vitro and in vivo potential metabolites of novel cardiovascular and adrenolytic drugs by liquid chromatography-mass spectrometry with the aid of experimental design." Nova Biotechnologica et chimica 18, no. 2: 179-194.

Validation study
Published: 01 November 2019 in Journal of Pharmaceutical and Biomedical Analysis
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Electrochemistry (EC) coupled with liquid chromatography and tandem mass spectrometry (HPLC/ESI-MS/MS) was used to study the oxidation products of cyclosporine A (CYC), everolimus (EWE), mycophenolic acid (MFA), sirolimus (SIR) and tacrolimus (TAK). Mimicry of the oxidative phase I and II metabolism was achieved in a thin-layer cell equipped with a boron doped diamond (BDD) working electrode. Structures of the electrochemically generated products were elucidated on the basis of MS/MS experiments. Additionally, a sensitive, specific and rapid HPLC-MS/MS method has been developed and validated for the quantification of selected drugs and their metabolites in human serum. Sample preparation was accomplished through microextraction by packed sorbent (MEPS) process. Finally, the identification of electrochemical products was done and results were compared with known metabolites of studied immunosuppressant drugs. Electrochemical conversion of target compounds has appeared as a new pseudo-in vitro instrumental technology for the prediction of potential metabolites, since the oxidation products have also been identified in serum samples.

ACS Style

Malgorzata Szultka-Mlynska; Boguslaw Buszewski. Electrochemical oxidation of selected immunosuppressants and identification of their oxidation products by means of liquid chromatography and tandem mass spectrometry (EC-HPLC-MS/MS). Journal of Pharmaceutical and Biomedical Analysis 2019, 176, 112799 .

AMA Style

Malgorzata Szultka-Mlynska, Boguslaw Buszewski. Electrochemical oxidation of selected immunosuppressants and identification of their oxidation products by means of liquid chromatography and tandem mass spectrometry (EC-HPLC-MS/MS). Journal of Pharmaceutical and Biomedical Analysis. 2019; 176 ():112799.

Chicago/Turabian Style

Malgorzata Szultka-Mlynska; Boguslaw Buszewski. 2019. "Electrochemical oxidation of selected immunosuppressants and identification of their oxidation products by means of liquid chromatography and tandem mass spectrometry (EC-HPLC-MS/MS)." Journal of Pharmaceutical and Biomedical Analysis 176, no. : 112799.

Journal article
Published: 02 October 2019 in Toxins
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Alkaloids have protective functions for plants and can play an important role in living organisms. Alkaloids may have a wide range of pharmacological activities. Many of them have cytotoxic activity. Nowadays, cancer has become a serious public health problem. Searching for effective drugs with anticancer activity is one of the most significant challenges of modern scientific research. The aim of this study was the investigation of cytotoxic activity of extracts obtained from Corydalis lutea root and herb, Dicentra spectabilis root and herb, Fumaria officinalis, Macleaya cordata leaves and herb, Mahonia aquifolia leaves and cortex, Meconopsis cambrica root and herb on FaDu, SCC-25, MCF-7, and MDA-MB-231 cancer cell lines. The cytotoxic activity of these extracts has not been previously tested for these cell lines. The aim was also to quantify selected alkaloids in the investigated extracts by High Performance Liquid Chromatography (HPLC). The analyses of alkaloid content were performed using HPLC in reversed phase (RP) mode using Polar RP column and mobile phase containing acetonitrile, water and ionic liquid (IL). Cytotoxic effect of the tested plant extracts and respective alkaloid standards were examined using human pharyngeal squamous carcinoma cells (FaDu), human tongue squamous carcinoma cells (SCC-25), human breast adenocarcinoma cell line (MCF-7), human triple-negative breast adenocarcinoma cell line (MDA-MB-231). All investigated plant extracts possess cytotoxic activity against tested cancer cell lines: FaDu, SCC-25, MCF-7, and MDA-MB-231. The highest cytotoxic activity against FaDu, SCC-25, and MCF-7 cell lines was estimated for Macleaya cordata leaf extract, while the highest cytotoxic activity against MDA-MB-231 cell line was obtained for Macleaya cordata herb extract. Differences in cytotoxic activity were observed for extracts obtained from various parts of investigated plants. In almost all cases the cytotoxic activity of investigated plant extracts, especially at the highest concentration against tested cell lines was significantly higher than the activity of anticancer drug etoposide. Our investigations exhibit that these plant extracts can be recommended for further in vivo experiments to confirm their anticancer activity.

ACS Style

Anna Petruczynik; Tomasz Plech; Tomasz Tuzimski; Justyna Misiurek; Barbara Kaproń; Dorota Misiurek; Małgorzata Szultka-Młyńska; Bogusław Buszewski; Monika Waksmundzka-Hajnos. Determination of Selected Isoquinoline Alkaloids from Mahonia aquifolia; Meconopsis cambrica; Corydalis lutea; Dicentra spectabilis; Fumaria officinalis; Macleaya cordata Extracts by HPLC-DAD and Comparison of Their Cytotoxic Activity. Toxins 2019, 11, 575 .

AMA Style

Anna Petruczynik, Tomasz Plech, Tomasz Tuzimski, Justyna Misiurek, Barbara Kaproń, Dorota Misiurek, Małgorzata Szultka-Młyńska, Bogusław Buszewski, Monika Waksmundzka-Hajnos. Determination of Selected Isoquinoline Alkaloids from Mahonia aquifolia; Meconopsis cambrica; Corydalis lutea; Dicentra spectabilis; Fumaria officinalis; Macleaya cordata Extracts by HPLC-DAD and Comparison of Their Cytotoxic Activity. Toxins. 2019; 11 (10):575.

Chicago/Turabian Style

Anna Petruczynik; Tomasz Plech; Tomasz Tuzimski; Justyna Misiurek; Barbara Kaproń; Dorota Misiurek; Małgorzata Szultka-Młyńska; Bogusław Buszewski; Monika Waksmundzka-Hajnos. 2019. "Determination of Selected Isoquinoline Alkaloids from Mahonia aquifolia; Meconopsis cambrica; Corydalis lutea; Dicentra spectabilis; Fumaria officinalis; Macleaya cordata Extracts by HPLC-DAD and Comparison of Their Cytotoxic Activity." Toxins 11, no. 10: 575.

Book chapter
Published: 13 September 2019 in Solid-Phase Extraction
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Inorganic oxide sorbents can function as polar phase sorbents for extraction from low-polarity solvents, as ion-exchange sorbents for the extraction of ions from (usually) aqueous solution, and after surface modification as low-polarity sorbents for the extraction of neutral compounds from aqueous solution. They dominate the practice of solid-phase extraction in column, cartridge, disk, pipette tip, and multiwell plate formats. Methods employed for the synthesis and characterization of sorbent particles are described and related to the performance of sorbent materials for extraction.

ACS Style

Małgorzata Szultka-Młyńska; Hossam Al-Suod; Bogusław Buszewski. Inorganic oxide and chemically bonded sorbents. Solid-Phase Extraction 2019, 37 -54.

AMA Style

Małgorzata Szultka-Młyńska, Hossam Al-Suod, Bogusław Buszewski. Inorganic oxide and chemically bonded sorbents. Solid-Phase Extraction. 2019; ():37-54.

Chicago/Turabian Style

Małgorzata Szultka-Młyńska; Hossam Al-Suod; Bogusław Buszewski. 2019. "Inorganic oxide and chemically bonded sorbents." Solid-Phase Extraction , no. : 37-54.

Original
Published: 29 November 2018 in Chromatographia
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The developed capillary electrophoresis (CE) method was applied to evaluate the aggregation activity of divalent metal ions (zinc) on bacterial cells. In our study, the influence of the Lactococcus lactis surface modification by zinc ions at different concentration (1, 3 and 10 mM, respectively) was determined. Electrophoretic results in pseudo isotachophoretic mode indicated that using a Znaq2+ caused the uncontrolled aggregation of bacterial cells and, as a result, new signals of microbial agglomerates were observed in the electropherogram. Moreover, the observed process is strongly related with the concentration of the used Znaq2+ solution—the number of bacterial agglomerates increased with an increasing concentration of modifier. The phenomenon of L. lactis uncontrolled clumping was also evaluated by application of size distribution and spectrometric in infrared range measurements (FT-IR) measurements. The obtained data indicated that the main groups involved in the bacterial cells aggregation process are deprotonated carboxyl and amid groups derived from microbial surface proteins. As a consequence, the proposed mechanism of the L. lactis uncontrolled clumping process is presented. In addition, data from fluorescence microscopy pointed out damage cells analyzed direct after electroanalysis. Therefore, CE may be a potential method for evaluating the aggregation activity of different types of bivalent metal ions against microbial cells.

ACS Style

B. Buszewski; A. Król; Pawel Pomastowski; V. Railean-Plugaru; Małgorzata Szultka-Młyńska. Electrophoretic Determination of Lactococcus lactis Modified by Zinc Ions. Chromatographia 2018, 82, 347 -355.

AMA Style

B. Buszewski, A. Król, Pawel Pomastowski, V. Railean-Plugaru, Małgorzata Szultka-Młyńska. Electrophoretic Determination of Lactococcus lactis Modified by Zinc Ions. Chromatographia. 2018; 82 (1):347-355.

Chicago/Turabian Style

B. Buszewski; A. Król; Pawel Pomastowski; V. Railean-Plugaru; Małgorzata Szultka-Młyńska. 2018. "Electrophoretic Determination of Lactococcus lactis Modified by Zinc Ions." Chromatographia 82, no. 1: 347-355.

Journal article
Published: 06 July 2018 in Journal of Chromatography B
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In this study electrochemistry (EC) coupled with electrospray ionization mass spectrometry (ESI-MS) was used to study the metabolic fate of three novel cardiovascular drugs: rivaroxaban (RIV), aliskiren (ALS), and prasugrel (PRS). Mimicry of the oxidative phase I metabolism was achieved in a simple amperometric thin-layer cell equipped with a boron-doped diamond (MD) working electrode. Structures of the electrochemically-generated metabolites were elucidated from MS/MS experiments. Additionally, a sensitive, specific, and rapid ultra-high performance liquid chromatography–tandem mass spectrometer (UHPLC–MS/MS) method has been developed and validated for the selected drugs in human urine samples. Three different sample preparation methods were compared and finally, sample preparation was accomplished through an ultrasound-assisted emulsification microextraction process (USAEME). The drugs were detected using a triple quadrupole tandem mass spectrometer by multiple reaction monitoring via an electrospray ionization source with positive ionization mode (ESI(+)). The results obtained by EC–MS were compared with conventional in vivo studies by analyzing urine samples from patients. Data from in vivo experiments showed good agreement with the data from electrochemical oxidation. Thus, EC–MS is very well-suited for the simulation of the oxidative metabolism of rivaroxaban, aliskiren, and prasugrel as well. Moreover, electrochemical conversion of target compounds appears to be a new in vitro technology for the prediction of potential metabolites.

ACS Style

Małgorzata Szultka-Młyńska; Sylwia Bajkacz; Magdalena Kaca; Irena Baranowska; Bogusław Buszewski. Electrochemical simulation of three novel cardiovascular drugs phase I metabolism and development of a new method for determination of them by liquid chromatography coupled with tandem mass spectrometry. Journal of Chromatography B 2018, 1093-1094, 100 -112.

AMA Style

Małgorzata Szultka-Młyńska, Sylwia Bajkacz, Magdalena Kaca, Irena Baranowska, Bogusław Buszewski. Electrochemical simulation of three novel cardiovascular drugs phase I metabolism and development of a new method for determination of them by liquid chromatography coupled with tandem mass spectrometry. Journal of Chromatography B. 2018; 1093-1094 ():100-112.

Chicago/Turabian Style

Małgorzata Szultka-Młyńska; Sylwia Bajkacz; Magdalena Kaca; Irena Baranowska; Bogusław Buszewski. 2018. "Electrochemical simulation of three novel cardiovascular drugs phase I metabolism and development of a new method for determination of them by liquid chromatography coupled with tandem mass spectrometry." Journal of Chromatography B 1093-1094, no. : 100-112.

Journal article
Published: 08 May 2018 in Scientific Reports
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The effect of a different pH on Saccharomyces cerevisiae cells modified with calcium ions was investigated by the capillary zone electrophoresis technique. For the identification of the wild strain of S. cerevisiae, the ribosomal nucleic acid sequencing and internal transcribed spacer sequencing as well as spectrometric approach were applied. The potentiometric titration and Fourier transform infrared spectroscopy have shown the occurrence of active functional groups such as carboxyl, amine/hydroxyl, phosphate/hydrogen phosphate groups on the surface of native yeast cells. Moreover, the spectroscopy study in a medium infrared range was carried out to identify the functional groups of yeast cells that participate in calcium ions binding interaction. Furthermore, the microscopic and spectrometric analysis shows that the pH value of the calcium ions solution has a significant effect on the intensity yeast cells clumping. Additionally, the impact of yeast cell clumping on the electrophoretic behaviours was examined. The modification of surface functional groups by calcium ions significantly affected the efficiency of electrophoretic separation. However, these changes did not affect the accuracy of S. cerevisiae identification by MALDI equipment with BioTyper platform. These results form the analytical solution for coupling of electrophoresis and MALDI-TOF MS technique.

ACS Style

Agnieszka Rogowska; Paweł Pomastowski; Michal Zloch; Viorica Railean-Plugaru; Anna Król; Katarzyna Rafińska; Małgorzata Szultka-Młyńska; Bogusław Buszewski. The influence of different pH on the electrophoretic behaviour of Saccharomyces cerevisiae modified by calcium ions. Scientific Reports 2018, 8, 1 -10.

AMA Style

Agnieszka Rogowska, Paweł Pomastowski, Michal Zloch, Viorica Railean-Plugaru, Anna Król, Katarzyna Rafińska, Małgorzata Szultka-Młyńska, Bogusław Buszewski. The influence of different pH on the electrophoretic behaviour of Saccharomyces cerevisiae modified by calcium ions. Scientific Reports. 2018; 8 (1):1-10.

Chicago/Turabian Style

Agnieszka Rogowska; Paweł Pomastowski; Michal Zloch; Viorica Railean-Plugaru; Anna Król; Katarzyna Rafińska; Małgorzata Szultka-Młyńska; Bogusław Buszewski. 2018. "The influence of different pH on the electrophoretic behaviour of Saccharomyces cerevisiae modified by calcium ions." Scientific Reports 8, no. 1: 1-10.

Journal article
Published: 12 April 2018 in Journal of Chromatography B
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A sensitive, rapid and specific analytical method using high performance liquid chromatography coupled with mass spectrometry (HPLC-QqQ-MS) was developed to determine selected antibiotic drugs and their metabolites (amoxicillin, cefotaxime, ciprofloxacin, clindamycin and metronidazole; amoxycilloic acid, 4-hydroxyphenyl glycyl amoxicillin, desacetyl cefotaxime, 3-desacetyl cefotaxime lactone, ciprofloxacin N-oxide, N-demethylclindamycin, clindamycin sulfoxide, and hydroxy metronidazole) in human whole blood and vascularized tissue after single oral administration. The samples were prepared by solid phase microextraction with C18 fibers (SPMEC18) and determined on a GRACE analytical C18 column, Vision HT (50 × 2 mm, 1.5 μm) at the flow rate of 0.4 mL min−1 using water and acetonitrile (containing 0.1% formic acid) as the mobile phase. The proposed method was successfully applied in a pharmacokinetic study of the selected antibiotic drugs and their metabolites in real human samples. Additionally, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS) was used for identification and qualification analysis of the target compounds.

ACS Style

Malgorzata Szultka-Mlynska; Pawel Pomastowski; Boguslaw Buszewski. Application of solid phase microextraction followed by liquid chromatography-mass spectrometry in the determination of antibiotic drugs and their metabolites in human whole blood and tissue samples. Journal of Chromatography B 2018, 1086, 153 -165.

AMA Style

Malgorzata Szultka-Mlynska, Pawel Pomastowski, Boguslaw Buszewski. Application of solid phase microextraction followed by liquid chromatography-mass spectrometry in the determination of antibiotic drugs and their metabolites in human whole blood and tissue samples. Journal of Chromatography B. 2018; 1086 ():153-165.

Chicago/Turabian Style

Malgorzata Szultka-Mlynska; Pawel Pomastowski; Boguslaw Buszewski. 2018. "Application of solid phase microextraction followed by liquid chromatography-mass spectrometry in the determination of antibiotic drugs and their metabolites in human whole blood and tissue samples." Journal of Chromatography B 1086, no. : 153-165.

Journal article
Published: 01 February 2018 in Journal of Microbiology, Immunology and Infection
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In this study, an acidophilic actinobacteria strain was used as a novel reducing agent for a single-step synthesis of nanostructure silver particles. We used a Streptacidiphilus durhamensis HGG16n isolate for efficient synthesis of bioactive silver nanoparticles [bio(AgNPs)] in an inexpensive, eco-friendly, and nontoxic manner. The obtained bio(AgNPs) exhibited unique physicochemical and biochemical properties.Structural, morphological, and optical properties of the synthesized biocolloids were characterized by spectroscopy, dynamic light scattering, and electron microscopy approaches. The antimicrobial activity was evaluated using the well- and disc-diffusion methods.The obtained crystalline structure and stable biosynthesized silver nanoparticles ranged in size from 8 nm to 48 nm and were mostly spherical in shape. Antimicrobial assays of the silver nanoparticles against pathogenic bacteria showed the highest antimicrobial activity against Pseudomonas aeruginosa, Staphylococcus aureus, and Proteus mirabilis, followed by Escherichia coli, Klebsiella pneumoniae, and Bacillus subtilis. Moreover, the synergistic effect of bio(AgNPs) with various commercially available antibiotics was also evaluated.These results provide insight into the development of new antimicrobial agents along with synergistic enhancement of the antibacterial mechanism against clinical bacteria.

ACS Style

Bogusław Buszewski; Viorica Railean-Plugaru; Paweł Pomastowski; Katarzyna Rafińska; Małgorzata Szultka-Młyńska; Patrycja Golinska; Magdalena Wypij; Dariusz Laskowski; Hanna Dahm. Antimicrobial activity of biosilver nanoparticles produced by a novel Streptacidiphilus durhamensis strain. Journal of Microbiology, Immunology and Infection 2018, 51, 45 -54.

AMA Style

Bogusław Buszewski, Viorica Railean-Plugaru, Paweł Pomastowski, Katarzyna Rafińska, Małgorzata Szultka-Młyńska, Patrycja Golinska, Magdalena Wypij, Dariusz Laskowski, Hanna Dahm. Antimicrobial activity of biosilver nanoparticles produced by a novel Streptacidiphilus durhamensis strain. Journal of Microbiology, Immunology and Infection. 2018; 51 (1):45-54.

Chicago/Turabian Style

Bogusław Buszewski; Viorica Railean-Plugaru; Paweł Pomastowski; Katarzyna Rafińska; Małgorzata Szultka-Młyńska; Patrycja Golinska; Magdalena Wypij; Dariusz Laskowski; Hanna Dahm. 2018. "Antimicrobial activity of biosilver nanoparticles produced by a novel Streptacidiphilus durhamensis strain." Journal of Microbiology, Immunology and Infection 51, no. 1: 45-54.

Journal article
Published: 01 January 2018 in Talanta
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In the last few years, a number of studies were conducted which aimed at understanding the mechanisms of cardiovascular drug, metabolism, and there is still the need to determine the metabolites of cardiac drugs for the purpose of metabolism control. In this study, we employ a direct combination of electrochemical oxidation and mass spectrometric (EC-MS) identification for monitoring the oxidation pathway of ten cardiovascular drugs (metoprolol, propranolol, propafenone, mexiletine, oxprenolol, pirbuterol, pindolol, cicloprolol, acebutolol and atenolol). Oxidation was accomplished in an electrochemical thin-layer cell coupled on-line to electrospray ionization mass spectrometry (EC/ESI-MS). For further characterization of electrochemical products, the approach involving liquid chromatography linked to tandem mass spectrometry was used. Appropriate conditions for oxidation and identification processes with such parameters as the potential value, mobile phase (type and pH) and working electrode were optimized. Optimization was performed with the use of central composite design (CCD). Besides electrochemical oxidation of analytes (phase I of metabolic transformation), addition of glutathione (GSH) for follow-up reactions (phase II conjunction) was also investigated. The electrochemical results were compared to in-vivo experiments by analyzing plasma and urine samples from patients who had been administered selected cardiovascular drugs. These results show that electrochemistry coupled to mass spectrometry turned out to be an analytical tool suitable to procure a feasible analytical base for the envisioned in vivo experiments.

ACS Style

Małgorzata Szultka-Młyńska; Sylwia Bajkacz; Irena Baranowska; Bogusław Buszewski. Structural characterization of electrochemically and in vivo generated potential metabolites of selected cardiovascular drugs by EC-UHPLC/ESI-MS using an experimental design approach. Talanta 2018, 176, 262 -276.

AMA Style

Małgorzata Szultka-Młyńska, Sylwia Bajkacz, Irena Baranowska, Bogusław Buszewski. Structural characterization of electrochemically and in vivo generated potential metabolites of selected cardiovascular drugs by EC-UHPLC/ESI-MS using an experimental design approach. Talanta. 2018; 176 ():262-276.

Chicago/Turabian Style

Małgorzata Szultka-Młyńska; Sylwia Bajkacz; Irena Baranowska; Bogusław Buszewski. 2018. "Structural characterization of electrochemically and in vivo generated potential metabolites of selected cardiovascular drugs by EC-UHPLC/ESI-MS using an experimental design approach." Talanta 176, no. : 262-276.

Journal article
Published: 01 September 2017 in Acta Chromatographica
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Karol Wróblewski; A. Petruczynik; B. Buszewski; Małgorzata Szultka-Młyńska; Hanna Karakula-Juchnowicz; M. Waksmundzka-Hajnos. Determination of vortioxetine in human serum and saliva samples by HPLC–DAD and HPLC–MS. Acta Chromatographica 2017, 29, 325 -344.

AMA Style

Karol Wróblewski, A. Petruczynik, B. Buszewski, Małgorzata Szultka-Młyńska, Hanna Karakula-Juchnowicz, M. Waksmundzka-Hajnos. Determination of vortioxetine in human serum and saliva samples by HPLC–DAD and HPLC–MS. Acta Chromatographica. 2017; 29 (3):325-344.

Chicago/Turabian Style

Karol Wróblewski; A. Petruczynik; B. Buszewski; Małgorzata Szultka-Młyńska; Hanna Karakula-Juchnowicz; M. Waksmundzka-Hajnos. 2017. "Determination of vortioxetine in human serum and saliva samples by HPLC–DAD and HPLC–MS." Acta Chromatographica 29, no. 3: 325-344.

Validation study
Published: 29 June 2017 in Analytical and Bioanalytical Chemistry
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Betaine is one of most studied biologically active compounds, due its role in the main biological processes. Although it may be found in several plants and roots, such as the Beta vulgaris family, present in typical diets, just a few analytical methods have been developed for its extraction from roots. A new, quick and effective procedure for the isolation and determination of betaine from two different varieties of B. vulgaris (red and gold) is presented. For betaine extraction, an accelerated solvent extraction (ASE) was coupled with solid-phase extraction. For betaine determination, a separation method based on hydrophilic interaction chromatography coupled with tandem mass spectrometry was optimized for a sensible detection of betaine by means of experimental design. Recoveries were about 93%, with RSD <5%, for both the matrices, without evidence of interfering species. The total content of betaine in extracts of various parts of plants (juice, peel, root) have been determined, obtaining concentrations in the range 3000–4000 mg/L for the juice and in the range 2–5 mg/g for the pulp and for the peel. The B. vulgaris gold species exhibited a higher concentration of betaine, compared to the red variety. Additionally, a micro extraction by packed sorbent technique and a modified quick, easy, cheap, rugged and safe (QuEChERS) procedure, were also tested and compared. Despite the lower recoveries of the latter, with respect to the ASE/SPE procedure (75–89%, RSD <1.5%), the ease of the method, which can be applied without the SPE purification procedure, can represent a positive improvement. Determination of betaine from Beta vulgaris samples.

ACS Style

Luca Rivoira; Sylwia Studzińska; Małgorzata Szultka-Młyńska; Maria Concetta Bruzzoniti; Bogusław Buszewski. New approaches for extraction and determination of betaine from Beta vulgaris samples by hydrophilic interaction liquid chromatography-tandem mass spectrometry. Analytical and Bioanalytical Chemistry 2017, 409, 5133 -5141.

AMA Style

Luca Rivoira, Sylwia Studzińska, Małgorzata Szultka-Młyńska, Maria Concetta Bruzzoniti, Bogusław Buszewski. New approaches for extraction and determination of betaine from Beta vulgaris samples by hydrophilic interaction liquid chromatography-tandem mass spectrometry. Analytical and Bioanalytical Chemistry. 2017; 409 (21):5133-5141.

Chicago/Turabian Style

Luca Rivoira; Sylwia Studzińska; Małgorzata Szultka-Młyńska; Maria Concetta Bruzzoniti; Bogusław Buszewski. 2017. "New approaches for extraction and determination of betaine from Beta vulgaris samples by hydrophilic interaction liquid chromatography-tandem mass spectrometry." Analytical and Bioanalytical Chemistry 409, no. 21: 5133-5141.

Validation study
Published: 01 May 2017 in Acta Poloniae Pharmaceutica - Drug Research
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Anna Petruczynik; Karol Wróblewski; Małgorzata Szultka-Młyńska; Boguslaw Buszewsk; Hanna Karakula-Juchnowicz; Jacek Gajewski; Justyna Morylowska-Topolska; Monika Waksmundzka-Hajnosi. DETERMINATION OF VENLAFAXINE, VILAZODONE AND THEIR MAIN ACTIVE METABOLITES IN HUMAN SERUM BY HPLC-DAD AND HPLC-MS. Acta Poloniae Pharmaceutica - Drug Research 2017, 74, 765 -775.

AMA Style

Anna Petruczynik, Karol Wróblewski, Małgorzata Szultka-Młyńska, Boguslaw Buszewsk, Hanna Karakula-Juchnowicz, Jacek Gajewski, Justyna Morylowska-Topolska, Monika Waksmundzka-Hajnosi. DETERMINATION OF VENLAFAXINE, VILAZODONE AND THEIR MAIN ACTIVE METABOLITES IN HUMAN SERUM BY HPLC-DAD AND HPLC-MS. Acta Poloniae Pharmaceutica - Drug Research. 2017; 74 (3):765-775.

Chicago/Turabian Style

Anna Petruczynik; Karol Wróblewski; Małgorzata Szultka-Młyńska; Boguslaw Buszewsk; Hanna Karakula-Juchnowicz; Jacek Gajewski; Justyna Morylowska-Topolska; Monika Waksmundzka-Hajnosi. 2017. "DETERMINATION OF VENLAFAXINE, VILAZODONE AND THEIR MAIN ACTIVE METABOLITES IN HUMAN SERUM BY HPLC-DAD AND HPLC-MS." Acta Poloniae Pharmaceutica - Drug Research 74, no. 3: 765-775.