Surface soil samples were collected near the Open Pit Bor (S1) and Open Pit Cerovo (S2), a grassland along the Borska Reka River (S3) and an unpolluted garden near Slatina village (reference site). Spontaneous plants (dandelion, nettle, coltsfoot, and creeping buttercup) and vegetables (onion, garlic, carrot, parsley, celery, potatoes, dill, and sorrel) were obtained from the former three sites and the reference site, respectively. The samples were analyzed for Zn, Cu, Fe, Mn, and Pb via FAAS. Pollution indices indicated low-to-moderate soil contamination at sites S1, S2, and S3. Cu was the main contaminant of environmental concern, being above the maximum admitted concentration at site S1. Metal levels in spontaneous plants were below phytotoxic levels. Cu content of leafy vegetables and celery roots and Pb content of most vegetables were not safe for human consumption. Metal concentrations tended to be significantly lower in plants than in soils, with only Cu occurring at significantly elevated levels in celery roots and sorrel leaves. Non-carcinogenic risk assessment showed that consumption of carrot roots and especially celery roots grown on unpolluted soils from the Bor area might pose long-term health risks for females and males, with the main contributors being Cu and Fe.

Herbicides are being used more and more to increase productivity in agriculture, but their excessive has been shown to lead to adverse effects on the environment, especially on the soil. Within this study the effect of the herbicide S-metolachlor on the activity of several enzymes (dehydrogenase, protease, phosphatase and urease) found in the loamy-clayey soil has been analyzed. There were seven experimental variants corresponding to the untreated soil and to the application of six distinct doses of S-metolachlor on soil samples maintained in laboratory conditions. Biochemical analyzes have been performed to assess the activities of the investigated enzymes at 7, 14 and 21 days, respectively, and several physiochemical parameters of the soil have been also monitored during these experiments. The data obtained in the experimental determinations were subjected to statistical analysis such as to evaluate if the changes in the activities of enzymes are significant and to establish if there are correlations between the evolution of analyzed enzymatic activities and the physicochemical parameters of the soil. Molecular docking approach has been also used to assess the interactions between the herbicide and investigated enzymes. The activities of studied enzymes decreased in a dose-dependent manner when the herbicide was applied. More than 21 days were necessary to recover the activities of dehydrogenase and protease in the soil treated with S-metolachlor, the activity of protease was recovered after 14 days and the activity of urease was recuperated after 7 days of incubation. This study also emphasized significant correlations between the enzymatic activities and some physicochemical parameters of the soil: pH, moisture, organic matter, nitrogen level and available phosphorus.

Aclonifen is an herbicide with a global market that is expected to grow in the period 2021–2027. This study focuses on revealing the effects of both aclonifen and of its soil metabolites on aqueous and soil environments. The soil’s physicochemical parameters and activities of enzymes found in the soil (urease, dehydrogenase, catalase, alkaline phosphatase) were evaluated in laboratory conditions for soil treated with various doses of aclonifen (from half of a normal dose, 2.1 g/ha, to three times the normal dose) every 7 days for a period of 28 days. A computational approach was used to calculate the acute toxicity of aclonifen and of its soil metabolites on aqueous organisms and to evaluate the possible interactions of these compounds with soil enzymes. The results obtained in the experimental study showed a significant inhibitory effect of the herbicide aclonifen on the investigated enzymes; those activities were not recovered after 28 days in the presence of high doses of aclonifen. Statistically significant effects of the herbicide dose and exposure duration on enzymes’ activities were observed using post hoc analysis at the p < 0.001 level. Pearson’s correlations revealed that the investigated enzyme activities were usually strongly (p < 0.001) influenced by the organic matter, available phosphorus content, and nitrogen level. The outcomes of the computational study revealed moderate acute aqueous toxicity of aclonifen and of its metabolites. All metabolites were also able to bind to the investigated enzymes and may have an inhibitory effect on their activities.

Milk and dairy products are among the foods preferred by consumers, as they are rich in nutrients, have high biological values, are easily accessible, and present a low risk to health. This study aimed to comparatively analyze the milk from sheep of the Turcana breed that were subjected to different feeding systems. The milk from the sheep was analyzed quantitatively and qualitatively; in this sense, the following were determined: daily milk production (DMY), physical parameters (pH, freezing point), chemical composition (lactose (L), fats (F), total proteins (TP), non-fat solids (Snf)), the content of heavy metals and trace elements (Zn, Cd, Cu, Fe, Mn, Pb), and microbiological parameters (the number of somatic cells (SCC), the total number of aerobic mesophilic germs that develop at 30 °C (NTG), the number of coliform bacteria (CT), the number of Staphylococcus aureus). Administration of the fodder complex produced, on the farm determined a slight quantitative increase in milk production, as well as in fat, protein and lactose content. The content of trace elements Zn, Fe, and Mn registered increases in milk samples from sheep that were administered the fodder complex. The content of heavy metals did not indicate any source of pollution in the grazing area. Furthermore, the microbiological parameters were within the allowed limits, indicating a good general state of health at the emergency level and the absence of microbiological contamination of the milk samples.

Zinc (Zn), copper (Cu), iron (Fe), manganese (Mn), cadmium (Cd), and lead (Pb) levels were measured in the Bor City water supply system (control) and two watercourses exposed to mining wastewaters, i.e., the Lutarica River (one site) and the Kriveljska River (two sites). The same parameters were determined in the brain, heart, lungs, stomach, liver, spleen, kidneys, and testes of male Wistar rats given water from these sources for 2 months. Water Cu, Fe, Cd, and Pb were outside the safe range, excepting the reference site. Significant impacts on intra-organ metal homeostasis were detected, especially in the brain, stomach, kidneys, and testes. The dynamics and magnitude of these changes (versus controls) depended on the target organ, analyzed metal, and water origin. The greatest number of significant intra-organ associations between essential and non-essential metals were found for Cd-Zn, Cd-Cu, and Cd-Mn. A regression analysis suggested the kidneys as the most relevant organ for monitoring water manganese, and the stomach and brain for lead. These results highlight the environmental risks associated with mining wastewaters from the Bor area and could help scientists in mapping the spatial distribution and severity of trace metal contamination in water sources.

Nicosulfuron, despite being a post-emergence herbicide commonly used in corn crops to combat weeds, there is still little information on nicosulfuron toxicity for soil microbiota. Little information exists on the impact of nicosulfuron on the enzymatic activities of soil dehydrogenases (Deh), urease (Ure), catalase (Cat), and alkaline phosphatase (Alp). We used a multiple dose- and time point (7, 14, 21, and 28 days) study design to determine the effect of nicosulfuron on these parameters during the first 28 days post-application. The soil pH, electrical conductivity (EC), organic matter content (OM), water content, ammonium-nitrogen (NH₄-N), nitrate-nitrogen (NO₃-N), and available phosphate were also monitored. Ure was the most responsive enzyme to nicosulfuron. This herbicide exerted a transitory dose- and time-dependent inhibitory effect on Deh activity; maximum inhibition occurred at 14 days at doses from the normal recommended dose onward. For Ure, the maximal inhibitory effect started at 7 days of exposure to half the normal recommended dose and continued for another 14 days. The effect on Cat occurred later, whereas Alp activity was affected by nicosulfuron between 7 and 21 days but only for triple the normal recommended dose. OM showed the most consistent relationships with these parameters, being strongly positively correlated with Deh, Cat, and Alp activities. These results indicate that nicosulfuron may alter the soil metabolic activity, thus affecting its fertility.