This paper evaluates the effect of construction quality defects on the seismic vulnerability of reinforced concrete (RC) frames. The variability in the construction quality of material properties and structural detailing is considered to assess the effect on the seismic behavior of RC frames. Concrete strength and yield strength of the reinforcement are selected as uncertain variables for the material properties, while the variabilities in the longitudinal reinforcement ratio and the volumetric ratio of transverse reinforcement are employed for structural detailing. Taking into account the selected construction quality uncertainties, the sensitivity analysis of the seismic vulnerability of the RC frames is performed and the impact of significant parameters is assessed at the global and local levels. This extensive analytical study reveals that the seismic vulnerability of the selected RC frame is particularly sensitive to concrete strength and the volumetric ratio of transverse reinforcement.