Remote sensing (RS) has been widely adopted as a tool to investigate several biotic and abiotic factors, directly and indirectly, related to biodiversity conservation. European grasslands are one of the most biodiverse habitats in Europe. Most of these habitats are subject to priority conservation measure, and several human-induced processes threaten them. The broad expansions of few dominant species are usually reported as drivers of biodiversity loss. In this context, using Sentinel-2 (S2) images, we investigate the distribution of one of the most spreading species in the Central Apennine: Brachypodium genuense. We performed a binary Random Forest (RF) classification of B. genuense using RS images and field-sampled presence/absence data. Then, we integrate the occurrences obtained from RS classification into species distribution models to identify the topographic drivers of B. genuense distribution in the study area. Lastly, the impact of B. genuense distribution in the Natura 2000 (N2k) habitats (Annex I of the European Habitat Directive) was assessed by overlay analysis. The RF classification process detected cover of B. genuense with an overall accuracy of 94.79%. The topographic species distribution model shows that the most relevant topographic variables that influence the distribution of B. genuense are slope, elevation, solar radiation, and topographic wet index (TWI) in order of importance. The overlay analysis shows that 74.04% of the B. genuense identified in the study area falls on the semi-natural dry grasslands. The study highlights the RS classification and the topographic species distribution model’s importance as an integrated workflow for mapping a broad-expansion species such as B. genuense. The coupled techniques presented in this work should apply to other plant communities with remotely recognizable characteristics for more effective management of N2k habitats.

Ailanthus altissima is an invasive alien species (IAS) present throughout Europe and included in the list of alien species of Union concern. In sub-Mediterranean areas of central Italy, there is a lack of knowledge about this invasive species and its interactions with the native forest ecosystems. We aim to find what are the main differences in vegetation structure and floristic diversity between A. altissima forests and native forests through the assessment of the principal ecological parameters that differ between the forest types. We performed 38 phytosociological relevés and sampling of ecological parameters in A. altissima forest communities and neighboring native forests. We analyzed how species richness, diversity, life forms, life strategies, structural characteristics, and ecological parameters changed in A. altissima forests compared with native ones. We found that in A. altissima forests, there is a shift in herbaceous layer richness, with a higher presence of annual ruderal herbs and the absence of herbaceous species linked to the forest environment. The ecological parameters that diverge from the native forests were total nitrogen, total carbon, and C/N ratio. A. altissima forest communities could threaten the biodiversity of the native forest ecosystems in the sub-Mediterranean landscape, favoring ruderal species and inhibiting the presence of typical forest species.

Reduction of soil greenhouse gas emissions is crucial to control increases in atmospheric CO₂ concentrations. Permanent grasslands are of considerable importance in climate change mitigation strategies as they cover about 13% of the global agricultural area. However, uncertainties remain for the effects of management practices on soil respiration, especially over the short term. This study investigated the influence of different mowing intensities on soil respiration over the short term for Bromus erectus-dominated grasslands in the central Apennines. From 2016 to 2018, soil respiration, temperature, and moisture were measured under three different management systems: customary management, intensive use, and abandonment. Both soil water content and temperature changed over time, however mowing did not affect soil water content while occasionally altered soil temperature. The intensive use promoted higher seasonal mean soil respiration compared to the abandonment only during the 2016 growing season. Soil temperature was the main driver of soil respiration above a soil water content threshold that varied little among treatments (18.23–22.71%). Below the thresholds, soil moisture was the main driver of soil respiration. These data suggest that different mowing regimes have little influence on soil respiration over the short term in Bromus erectus-dominated grasslands. Thus, more intensive use would not have significative impacts on soil respiration, at least over the short term. Future studies need to clarify the role of root mycorrhizal and microbial respiration in the light of climate change, considering the seasonal redistribution of the rainfall.

Permanent grasslands provide a wide array of ecosystem services. Despite this, few studies have investigated grassland carbon (C) dynamics, and especially those related to the effects of land-use changes. This study aimed to determine whether the land-use change from permanent grassland to arable lands resulted in variations in the soil C stock, and whether such variations were due to increased soil respiration or to management practices. To address this, seasonal variations of soil respiration, sensitivity of soil respiration to soil temperature (Q₁₀), and soil C stock variations generated by land-use changes were analyzed in a temperate mountain area of central Italy. The comparisons were performed for a permanent grassland and two adjacent fields, one cultivated with lentil and the other with emmer, during the 2015 crop year. Soil respiration and its heterotrophic component showed different spatial and temporal dynamics. Annual cumulative soil respiration rates were 6.05, 5.05 and 3.99 t C ha⁻¹ year⁻¹ for grassland, lentil and emmer, respectively. Both soil respiration and heterotrophic soil respiration were positively correlated with soil temperature at 10 cm depth. Derived Q₁₀ values were from 2.23 to 6.05 for soil respiration, and from 1.82 to 4.06 for heterotrophic respiration. Soil C stock at over 0.2 m in depth was 93.56, 48.74 and 46.80 t C ha⁻¹ for grassland, lentil and emmer, respectively. The land-use changes from permanent grassland to arable land lead to depletion in terms of the soil C stock due to water soil erosion. A more general evaluation appears necessary to determine the multiple effects of this land-use change at the landscape scale.