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Prof. Hana Sehadova
Biology Centre of the Czech Academy of Sciences, Institute of Entomology, České Budějovice, Czech Republic

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Research Keywords & Expertise

0 Confocal Laser Scanning Microscopy
0 Fluorescence Microscopy
0 Immunohistochemistry
0 In Situ Hybridization
0 Neurobiology

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Short Biography

Research Experience 2008-present Researcher, Department of Molecular Biology and Genetics, Institute of Entomology, Biology Centre, Academy of Sciences of the Czech Republic, Ceske Budejovice 2006-2008 Postdoctoral Research Assistant, School of Biological and Chemical Sciences, Queen Mary University, London, United Kingdom 2004-2006 The Japan Society for the Promotion of Science (JSPS) Postdoctoral Fellowship For Foreign Researchers, Division of Molecular Science, Graduate School of Science and Technology, Kobe University, Japan 2003-2004 Research Associate, Faculty of Agriculture, Kobe University, Kobe, Japan 1997-2003 Research Assistant, Institute of Entomology, Academy of Sciences of the Czech Republic, Ceske Budejovice

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Journal article
Published: 31 July 2021 in International Journal of Molecular Sciences
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Many lepidopteran larvae produce silk feeding shelters and cocoons to protect themselves and the developing pupa. As caterpillars evolved, the quality of the silk, shape of the cocoon, and techniques in forming and leaving the cocoon underwent a number of changes. The silk of Pseudoips prasinana has previously been studied using X-ray analysis and classified in the same category as that of Bombyx mori, suggesting that silks of both species have similar properties despite their considerable phylogenetic distance. In the present study, we examined P. prasinana silk using ‘omics’ technology, including silk gland RNA sequencing (RNA-seq) and a mass spectrometry-based proteomic analysis of cocoon proteins. We found that although the central repetitive amino acid sequences encoding crystalline domains of fibroin heavy chain molecules are almost identical in both species, the resulting fibers exhibit quite different mechanical properties. Our results suggest that these differences are most probably due to the higher content of fibrohexamerin and fibrohexamerin-like molecules in P. prasinana silk. Furthermore, we show that whilst P. prasinana cocoons are predominantly made of silk similar to that of other Lepidoptera, they also contain a second, minor silk type, which is present only at the escape valve.

ACS Style

Michal Rindos; Lucie Kucerova; Lenka Rouhova; Hana Sehadova; Michal Sery; Miluse Hradilova; Peter Konik; Michal Zurovec. Comparison of Silks from Pseudoips prasinana and Bombyx mori Shows Molecular Convergence in Fibroin Heavy Chains but Large Differences in Other Silk Components. International Journal of Molecular Sciences 2021, 22, 8246 .

AMA Style

Michal Rindos, Lucie Kucerova, Lenka Rouhova, Hana Sehadova, Michal Sery, Miluse Hradilova, Peter Konik, Michal Zurovec. Comparison of Silks from Pseudoips prasinana and Bombyx mori Shows Molecular Convergence in Fibroin Heavy Chains but Large Differences in Other Silk Components. International Journal of Molecular Sciences. 2021; 22 (15):8246.

Chicago/Turabian Style

Michal Rindos; Lucie Kucerova; Lenka Rouhova; Hana Sehadova; Michal Sery; Miluse Hradilova; Peter Konik; Michal Zurovec. 2021. "Comparison of Silks from Pseudoips prasinana and Bombyx mori Shows Molecular Convergence in Fibroin Heavy Chains but Large Differences in Other Silk Components." International Journal of Molecular Sciences 22, no. 15: 8246.

Journal article
Published: 19 January 2021 in Insect Biochemistry and Molecular Biology
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Many lepidopteran larvae produce silk secretions to build feeding tubes and cocoons that play important protective roles in their lives. Recent research on the silk of bombycoid and pyralid moths has shown that it contains several highly abundant silk components with remarkable mechanical properties. It was also found to contain a number of other proteins of which the functions have yet to be identified. To gain an overview of the silk composition in more primitive lepidopteran species and to identify the core silk components common to most species, we analyzed the cocoon proteins of Tineola bisselliella, which belongs to the basal ditrysian moth line. Using de novo transcriptome sequencing combined with mass spectrometry (MS)-based proteomics, we detected more than 100 secretory proteins in the silk cocoons. Fibroin, sericins, and protease inhibitors were found to be the most abundant proteins, along with several novel candidate silk components. We also verified the tissue and developmental stage specificity of the silk protein expression and characterized the morphology of both the silk glands and silk in T. bisselliella. Our study provides a detailed analysis of silk in the primitive moth, expands the known set of silk-specific genes in Lepidoptera, and helps to elucidate their evolutionary relationships.

ACS Style

Lenka Rouhova; Barbara Kludkiewicz; Hana Sehadova; Michal Sery; Lucie Kucerova; Peter Konik; Michal Zurovec. Silk of the common clothes moth, Tineola bisselliella, a cosmopolitan pest belonging to the basal ditrysian moth line. Insect Biochemistry and Molecular Biology 2021, 130, 103527 .

AMA Style

Lenka Rouhova, Barbara Kludkiewicz, Hana Sehadova, Michal Sery, Lucie Kucerova, Peter Konik, Michal Zurovec. Silk of the common clothes moth, Tineola bisselliella, a cosmopolitan pest belonging to the basal ditrysian moth line. Insect Biochemistry and Molecular Biology. 2021; 130 ():103527.

Chicago/Turabian Style

Lenka Rouhova; Barbara Kludkiewicz; Hana Sehadova; Michal Sery; Lucie Kucerova; Peter Konik; Michal Zurovec. 2021. "Silk of the common clothes moth, Tineola bisselliella, a cosmopolitan pest belonging to the basal ditrysian moth line." Insect Biochemistry and Molecular Biology 130, no. : 103527.

Journal article
Published: 11 December 2020 in Cells
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Insect adipokinetic hormones (AKHs) are short peptides produced in the corpora cardiaca and are responsible for mobilizing energy stores from the fat body to the hemolymph. Three related peptides, AKH1, AKH2, and AKH/corazonin-related peptide (ACP) as well as three AKH receptors have been reported in Bombyx mori. AKH1 and AKH2 are specific for the AKHR1 receptor, whereas ACP interacts with the other two AKHRs. To assess the effect of the two silkworm AKHs and ACP in the regulation of energy homeostasis we examined the expression pattern of the three peptides and their receptors as well as their effect on the level of carbohydrates and lipids in the hemolymph. Our results support the hypothesis that only AKH1 and AKH2 peptides together with the AKHR1 receptor are involved in the maintenance of energy homeostasis. Because Bombyx AKHR1 (BmAKHR1) seems to be a true AKHR we generated its mutation. The BmAKHR1 mutant larvae display significantly lower carbohydrate and lipid levels in the hemolymph and reduced sensitivity to starvation. Our study clarifies the role of BmAKHR1 in energy homeostasis.

ACS Style

Hana Sehadova; Yoko Takasu; Anna Zaloudikova; Yu-Hsien Lin; Ivo Sauman; Hideki Sezutsu; Lenka Rouhova; Dalibor Kodrik; Michal Zurovec. Functional Analysis of Adipokinetic Hormone Signaling in Bombyx mori. Cells 2020, 9, 2667 .

AMA Style

Hana Sehadova, Yoko Takasu, Anna Zaloudikova, Yu-Hsien Lin, Ivo Sauman, Hideki Sezutsu, Lenka Rouhova, Dalibor Kodrik, Michal Zurovec. Functional Analysis of Adipokinetic Hormone Signaling in Bombyx mori. Cells. 2020; 9 (12):2667.

Chicago/Turabian Style

Hana Sehadova; Yoko Takasu; Anna Zaloudikova; Yu-Hsien Lin; Ivo Sauman; Hideki Sezutsu; Lenka Rouhova; Dalibor Kodrik; Michal Zurovec. 2020. "Functional Analysis of Adipokinetic Hormone Signaling in Bombyx mori." Cells 9, no. 12: 2667.

Research article
Published: 19 February 2020 in PLOS ONE
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The late 5th instar caterpillar of the cecropia silk moth (Hyalophora cecropia) spins a silken cocoon with a distinct, multilayered architecture. The cocoon construction program, first described by the seminal work of Van der Kloot and Williams, consists of a highly ordered sequence of events. We perform behavioral experiments to re-evaluate the original cecropia work, which hypothesized that the length of silk that passes through the spinneret controls the orderly execution of each of the discrete events of cocoon spinning. We confirm and extend by three-dimensional scanning and quantitative measurements of silk weights that if cocoon construction is interrupted, upon re-spinning, the caterpillar continues the cocoon program from where it left off. We also confirm and extend by quantitative measurements of silk weights that cecropia caterpillars will not bypass any of the sections of the cocoon during the construction process, even if presented with a pre-spun section of a cocoon spun by another caterpillar. Blocking silk output inhibits caterpillars from performing normal spinning behaviors used for cocoon construction. Surprisingly, unblocking silk output 24-hr later did not restart the cocoon construction program, suggesting the involvement of a temporally-defined interval timer. We confirm with surgical reductions of the silk glands that it is the length of silk itself that matters, rather than the total amount of silk extracted by individuals. We used scanning electron microscopy to directly show that either mono- or dual-filament silk (i.e., equal silk lengths but which vary in their total amount of silk extracted) can be used to construct equivalent cocoons of normal size and that contain the relevant layers. We propose that our findings, taken together with the results of prior studies, strongly support the hypothesis that the caterpillar uses a silk “odometer” to measure the length of silk extracted during cocoon construction but does so in a temporally regulated manner. We further postulate that our examination of the anatomy of the silk spinning apparatus and ablating spinneret sensory output provides evidence that silk length measurement occurs upstream of output from the spinneret.

ACS Style

Hana Sehadova; Patrick A. Guerra; Ivo Sauman; Steven M. Reppert. A re-evaluation of silk measurement by the cecropia caterpillar (Hyalophora cecropia) during cocoon construction reveals use of a silk odometer that is temporally regulated. PLOS ONE 2020, 15, e0228453 .

AMA Style

Hana Sehadova, Patrick A. Guerra, Ivo Sauman, Steven M. Reppert. A re-evaluation of silk measurement by the cecropia caterpillar (Hyalophora cecropia) during cocoon construction reveals use of a silk odometer that is temporally regulated. PLOS ONE. 2020; 15 (2):e0228453.

Chicago/Turabian Style

Hana Sehadova; Patrick A. Guerra; Ivo Sauman; Steven M. Reppert. 2020. "A re-evaluation of silk measurement by the cecropia caterpillar (Hyalophora cecropia) during cocoon construction reveals use of a silk odometer that is temporally regulated." PLOS ONE 15, no. 2: e0228453.

Journal article
Published: 12 February 2020 in Insects
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Titanus giganteus is one of the largest insects in the world, but unfortunately, there is a lack of basic information about its biology. Previous papers have mostly described Titanus morphology or taxonomy, but studies concerning its anatomy and physiology are largely absent. Thus, we employed microscopic, physiological, and analytical methods to partially fill this gap. Our study focused on a detailed analysis of the antennal sensilla, where coeloconic sensilla, grouped into irregularly oval fields, and sensilla trichoidea were found. Further, the inspection of the internal organs showed apparent degeneration of the gut and almost total absence of fat body. The gut was already empty; however, certain activity of digestive enzymes was recorded. The brain was relatively small, and the ventral nerve cord consisted of three ganglia in the thorax and four ganglia in the abdomen. Each testis was composed of approximately 30 testicular follicles filled with a clearly visible sperm. Chromatographic analysis of lipids in the flight muscles showed the prevalence of storage lipids that contained 13 fatty acids, and oleic acid represented 60% of them. Some of our findings indicate that adult Titanus rely on previously accumulated reserves rather than feeding from the time of eclosion.

ACS Style

Jiří Dvořáček; Hana Sehadová; František Weyda; Aleš Tomčala; Markéta Hejníková; Dalibor Kodrík. First Comprehensive Study of a Giant among the Insects, Titanus giganteus: Basic Facts from Its Biochemistry, Physiology, and Anatomy. Insects 2020, 11, 120 .

AMA Style

Jiří Dvořáček, Hana Sehadová, František Weyda, Aleš Tomčala, Markéta Hejníková, Dalibor Kodrík. First Comprehensive Study of a Giant among the Insects, Titanus giganteus: Basic Facts from Its Biochemistry, Physiology, and Anatomy. Insects. 2020; 11 (2):120.

Chicago/Turabian Style

Jiří Dvořáček; Hana Sehadová; František Weyda; Aleš Tomčala; Markéta Hejníková; Dalibor Kodrík. 2020. "First Comprehensive Study of a Giant among the Insects, Titanus giganteus: Basic Facts from Its Biochemistry, Physiology, and Anatomy." Insects 11, no. 2: 120.

Journal article
Published: 03 December 2019 in Viruses
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A lichen body is formed most often from green alga cells trapped in a net of ascomycetous fungi and accompanied by endolichenic or parasitic fungi, other algae, and symbiotic or free-living bacteria. The lichen’s microcosmos is inhabited by mites, insects, and other animals for which the lichen is a source of food or a place to live. Novel, four-segmented dsRNA viruses were detected in saxicolous Chrysothrix chlorina and Lepraria incana lichens. Comparison of encoded genome proteins revealed classification of the viruses to the genus Alphachrysovirus and a relationship to chrysoviruses from filamentous ascomycetous fungi. We propose the names Chrysothrix chrysovirus 1 (CcCV1) and Lepraria chrysovirus 1 (LiCV1) as acronyms for these viruses. Surprisingly, observation of Chrysothrix chlorina hybridization with fluorescent-labelled virus probe by confocal microscope revealed that the CcCV1 virus is not present in the lichen body-forming fungus but in accompanying endolichenic Penicillium citreosulfuratum fungus. These are the first descriptions of mycoviruses from a lichen environment.

ACS Style

Karel Petrzik; Igor Koloniuk; Hana Sehadová; Tatiana Sarkisova. Chrysoviruses Inhabited Symbiotic Fungi of Lichens. Viruses 2019, 11, 1120 .

AMA Style

Karel Petrzik, Igor Koloniuk, Hana Sehadová, Tatiana Sarkisova. Chrysoviruses Inhabited Symbiotic Fungi of Lichens. Viruses. 2019; 11 (12):1120.

Chicago/Turabian Style

Karel Petrzik; Igor Koloniuk; Hana Sehadová; Tatiana Sarkisova. 2019. "Chrysoviruses Inhabited Symbiotic Fungi of Lichens." Viruses 11, no. 12: 1120.

Journal article
Published: 21 August 2019 in Parasites & Vectors
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In Europe, the tapeworm Dibothriocephalus latus (syn. Diphyllobothrium latum) is a well-known etiological agent of human diphyllobothriosis, which spreads by the consumption of raw fish flesh infected by plerocercoids (tapeworm’s larval stage). However, the process of parasite establishment in both intermediate and definitive hosts is poorly understood. This study was targeted mainly on the scolex (anterior part) of the plerocercoid of this species, which facilitates penetration of the parasite in intermediate paratenic fish hosts, and subsequently its attachment to the intestine of the definitive host. Plerocercoids were isolated from the musculature of European perch (Perca fluviatilis) caught in Italian alpine lakes. Parasites were examined using confocal microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Immunofluorescence tagging was held on whole mount larvae. The organisation of the central and peripheral nervous system was captured in D. latus plerocercoids, including the ultrastructure of the nerve cells possessing large dense neurosecretory granules. Two types of nerve fibres run from the body surface toward the nerve plexus located in the parenchyma on each side of bothria. One type of these fibres was found to be serotoninergic and possessed large subtegumental nerve cell bodies. A well-developed gland apparatus, found throughout the plerocercoid parenchyma, produced heterogeneous granules with lucent core packed in a dense layer. Three different types of microtriches occurred on the scolex and body surface of plerocercoids of D. latus: (i) uncinate spinitriches; (ii) coniform spinitriches; and (iii) capilliform filitriches. Non-ciliated sensory receptors were observed between the distal cytoplasm of the tegument and the underlying musculature. Confocal laser scanning microscopy and electron microscopy (SEM and TEM) showed the detailed microanatomy of the nervous system in the scolex of plerocercoids, and also several differences in the larval stages compared with adult D. latus. These features, i.e. well-developed glandular system and massive hook-shaped uncinate spinitriches, are thus probably required for plerocercoids inhabiting fish hosts and also for their post-infection attachment in the human intestine.

ACS Style

Daniel Barčák; Aneta Yoneva; Hana Sehadová; Mikuláš Oros; Andrea Gustinelli; Roman Kuchta. Complex insight on microanatomy of larval “human broad tapeworm” Dibothriocephalus latus (Cestoda: Diphyllobothriidea). Parasites & Vectors 2019, 12, 1 -17.

AMA Style

Daniel Barčák, Aneta Yoneva, Hana Sehadová, Mikuláš Oros, Andrea Gustinelli, Roman Kuchta. Complex insight on microanatomy of larval “human broad tapeworm” Dibothriocephalus latus (Cestoda: Diphyllobothriidea). Parasites & Vectors. 2019; 12 (1):1-17.

Chicago/Turabian Style

Daniel Barčák; Aneta Yoneva; Hana Sehadová; Mikuláš Oros; Andrea Gustinelli; Roman Kuchta. 2019. "Complex insight on microanatomy of larval “human broad tapeworm” Dibothriocephalus latus (Cestoda: Diphyllobothriidea)." Parasites & Vectors 12, no. 1: 1-17.

Journal article
Published: 02 April 2015 in European Journal of Entomology
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In insects, the crustacean cardioactive peptide (CCAP) is involved mainly in the regulation of ecdysis and is both a hormone secreted into the haemolymph and a neuromodulator or neurotransmitter in the nervous system. However, this peptide has only been recorded in the brains of a few species of insects. The present study examined the distribution of CCAP in the cephalic ganglia of the cockroach, Periplaneta americana, using two kinds of specific antibodies. The antibody directed against synthetic CCAP detected a large number of CCAP-immunoreactive neurons in the protocerebrum, deutocerebrum, tritocerebrum, suboesophageal ganglion, optic lobes, frontal ganglion and neurohemal organs, while the antibody directed against CCAP precursor (pre-propeptide) stained only a few neurons within the central brain and frontal ganglion. The latter antibody also revealed similar sets of neurons in another species of cockroach, Gromphadorhina portentosa, and identified the CCAP precursor of approximately 15 kDa in size in Western blot analyses.

ACS Style

Marcin Gladysz; Joanna Guzik; Hana Sehadová; Bartosz Baran; Jadwiga Bembenek; Bogdan Dolezych. Immunochemical detection of the crustacean cardioactive peptide in the cephalic ganglia of cockroaches (Blattodea: Blattidae). European Journal of Entomology 2015, 112, 235 -244.

AMA Style

Marcin Gladysz, Joanna Guzik, Hana Sehadová, Bartosz Baran, Jadwiga Bembenek, Bogdan Dolezych. Immunochemical detection of the crustacean cardioactive peptide in the cephalic ganglia of cockroaches (Blattodea: Blattidae). European Journal of Entomology. 2015; 112 (2):235-244.

Chicago/Turabian Style

Marcin Gladysz; Joanna Guzik; Hana Sehadová; Bartosz Baran; Jadwiga Bembenek; Bogdan Dolezych. 2015. "Immunochemical detection of the crustacean cardioactive peptide in the cephalic ganglia of cockroaches (Blattodea: Blattidae)." European Journal of Entomology 112, no. 2: 235-244.