Stevia rebaudiana is a natural sweetener herb that is increasingly used in herbal medicines in the food and cosmetics industries. Molecular methods can be combined with morphological techniques to identify stevia genotypes as a starting material to produce more reliable bioproducts. This study evaluated the level of the genetic and biochemical diversity in various stevia genotypes using HPLC (high performance liquid chromatography) analysis and random amplified polymorphic DNA (RAPD) markers. Stevia genotypes collected from different locations of the world showed clear variations at the biochemical and genetic level in Polish climate conditions. The influence of the genotypes on the content of steviol glycosides, antioxidants, phenols, flavonoids, and tannins was analyzed using phytochemical assays. Genotypes from Morocco, Poland, Egypt, and Nigeria can be defined as samples of higher quality compared to other genotypes analyzed in terms of the amount of steviol glycosides. Considering the rebaudioside A/stevioside ratio as a selection criterion, genotypes from Australia, China, India, and Pakistan should be considered to be valuable in terms of suitability for obtaining new varieties. The present results of RAPD marker analysis indicated differential banding pattern and considerable polymorphism among all ten stevia genotypes. Genotypes from Morocco, Egypt, Poland, Nigeria, China, and India, as genetically different, can be selected for further stevia breeding programs.

Many of the active constituents of drug or medicines were originally derived from medicinal plants. Iranian Borage are still being used in regular basis. Selenium (Se) is an essential mineral nutrient for animal and human growth. The aim of this research was to determine the effect of (2, 4, 8 and 16 mg L⁻¹) of as sodium selenate (Na₂SeO₄) and as sodium selenite (Na₂SeO₃) on some important properties of Iranian Borage in factorial based on Randomized Complete Block Design via four steps: 2 true leaves stage, ten leaves, 2 weeks and 1 week before flowering. The traits were evaluated during flowering period. Results showed that the highest shoot fresh and dry weight and shoot length, total alkaloid, essential oil percentage were obtained by 4 mg L⁻¹ sodium selenate at the end of flowering. In addition, 4 mg L⁻¹ sodium selenate concentration significantly improved flower yield (diameter, number, weight). The plants were treated with 8 mg L⁻¹ sodium selenate, the higher total phenols and flavonoids, antioxidant activity, soluble sugars, root and fresh weight was seen at end of flowering. When the plants were sprayed with 4 mg L⁻¹ sodium selenite higher total chlorophyll was observed at full of flowering. 16 mg L⁻¹ sodium selenite released the maximum Se acclimation in the petals. 20 composites were discovered containing ɑ-Pinene (23.61%) with sodium selenate in 4 mg L⁻¹. Generally, selenium sources significantly improved morpho-physiological and phytochemical.

It is necessary to develop a simple way to achieve food quality quantitatively. Nanotechnology is a key advanced technology enabling contribution, development, and sustainable impact on food, medicine, and agriculture. In terms of medicinal and therapeutic properties, Hypericumperforatum is an important species. For this study, a randomized complete block design with three replications was used in each experimental unit. The foliar application of selenite and nano-selenium (6, 8, 10, and 12 mg/l), control (distilled water), at the rosette stage and harvesting at 50% flowering stage has been applied as an alleviation strategy subjected to producing essential oils and antioxidant activity. Experimental results revealed that the selenite and nano selenium fertilizers had a significant effect on traits such as total weight of biomass, essential oil percentage, the content of hypericin and hyperforin, the selenium accumulation in the plant, relative leaf water content, chlorophylls, phenolic content, proline, catalase, peroxidase, malondialdehyde, and DPPH. The highest essential oil content was obtained from the control treatment when the accumulation of selenium was achieved with 12 mg/l nano-selenium. The maximum rate of hypericin was seen in the foliar application of 8 mg/l selenite whereas the maximum hyperforin was gained at 10 mg/l selenium. Conceding that the goal is to produce high hypericin/ hyperforin, and also the accumulation of selenium in the plant, treatments of 6 and 8 mg/l of selenite and nano-selenium could be applied. Consequently, an easy detection technique proposed herein can be successfully used in different ranges, including biology, medicine, and the food industry.

The phytochemical characteristic analysis of a group of 30 haskap berry genotypes was carried out bearing in mind the concern for the consumption of food with high nutraceutical value that helps maintain good health. Phytochemical fruit composition and antioxidant activity were assessed by the Folin–Ciocalteau, spectrophotometric, DPPH (1,1-diphenyl-2-picrylhydrazyl) as well as ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) method. Evaluation of antioxidant activity was referred to as the Trolox equivalent. The observed differences in the content of phenolics, flavonoids, vitamin C and antioxidant activity allowed us to select genotypes which, due to the high level of the analyzed compounds, are particularly recommended in everyone’s diet. In addition, the analysis of the prospects of increasing the analyzed phytochemical properties, estimated by parameters such as heritability and genetic progress, indicates the effectiveness of breeding in relation to each of the analyzed traits. The results of the presented research can be used in the implementation of future breeding programs for this valuable species.

There are 16 species in the genus Satureja L. (Lamiaceae), of which 10 are native. This research aimed to investigate the effect of gamma rays and storage conditions and duration on the percentage and components of the essential oil and some biochemical characteristics of Satureja mutica Fisch & C.A. Mey at the Research Institute of Forests and Rangelands. Plants were collected at the full flowering stage and exposed to different doses of gamma rays (0, 2.5, 5, 7.5, and 10 kGy) at the Atomic Energy Organization, Iran. The samples were kept in a refrigerator (4 °C) and in the shade (25 ± 2 °C) for 0, 120, and 240 h. This experiment was performed in a completely randomized design. Essential oil extraction was done by water distillation for 2 h. The composition of their essential oil components was identified using GC and GC/MS. Some biochemical traits, including phenol content, antioxidant capacity, and carbohydrate content, were measured. The results indicated that irradiation on the percentage of essential oil showed a statistically significant difference. In addition, the interaction effect of irradiation × storage conditions, irradiation × duration of storage, on the percentage of essential oil was significant. According to a comparison of the means, 2.5 kGy irradiation produced the highest percentage of essential oil (0.4%); in contrast, a significant decrease was detected in components with 7.5 and 10 kGy irradiation. It was observed that the percentage of some essential oil compounds decreased with the gamma-ray intensity increase. 2.5 kGy of gamma rays and shade storage conditions for 240 h led to the highest content of p-cymene and carvacrol. Nevertheless, the highest thymol content was obtained under refrigeration conditions without irradiation. The maximum phenol content and antioxidant capacity were obtained when the plants were irradiated with 2.5 and 7.5 kGy gamma rays. However, the maximum carbohydrate rate was observed in non-irradiated plants. It was concluded that low-intensity gamma rays could improve the percentage of essential oil and main components like p-cymene and carvacrol in S. mutica Fisch & C.A. Mey.