The present study aims to assess the influences of long-term crop straw returning and recommended potassium fertilization on the dynamic change in rice and oilseed rape yield, soil properties, bacterial and fungal alpha diversity, and community composition in a rice–oilseed rape system. A long-term (2011–2020) field experiment was carried out in a selected paddy soil farmland in Jianghan Plain, central China. There were four treatments with three replications: NP, NPK, NPS, and NPKS, where nitrogen (N), phosphate (P), potassium (K), and (S) denote N fertilizer, P fertilizer, K fertilizer, and crop straw, respectively. Results showed that long-term K fertilization and crop straw returning could increase the crop yield at varying degrees for ten years. Compared with the NP treatment, the long-term crop straw incorporation with K fertilizer (NPKS treatment) was found to have the best effect, and the yield rates increased by 23.0% and 20.5% for rice and oilseed rape, respectively. The application of NPK fertilizer for ten years decreased the bacterial and fungal alpha diversity and the relative abundance of dominant bacterial and fungal taxa, whereas continuous straw incorporation had a contradictory effect. NPKS treatment significantly increased the relative abundance of some copiotrophic bacteria (Firmicutes, Gemmatimonadetes, and Proteobacteria) and fungi (Ascomycota). Available K, soil organic matter, dissolved organic carbon, and easily oxidized organic carbon were closely related to alterations in the composition of the dominant bacterial community; easily oxidized organic carbon, dissolved organic carbon, and slowly available K were significantly correlated with the fungal community. We conclude that long-term crop straw returning to the field accompanied with K fertilizer should be employed in rice-growing regions to achieve not only higher crop yield but also the increase in soil active organic carbon and available K content and the improvement of the biological quality of farmland.

Tillage practices significantly influence soil nutrient retention, soil structure, and stability. However, the impact of tillage practices on soil stability and erosion resistance through the perturbation approach of soil structure remains unclear. This study aimed to establish universal principles across slope surface and soil profile scales. We evaluated the effects of various tillage practices, including conventional tillage (CT), soil compaction (CM), subsoil tillage (ST), no tillage (NT), and subsoil tillage and soil compaction (SCM) on soil stability and erosion resistance in China’s red soil hilly region. Soil stability, erosion resistance, and other soil properties were quantified using field surveys and laboratory experiments. We discovered significant variations in soil aggregate stability (SAS), wet aggregate stability (WAS), soil mechanical stability (SMS), and soil erodibility (SE) among the five tillage methods. The K factor’s average value indicated that the 0–40 cm soil layer was more erodible for CT (0.472) than for other methods, with NT (0.26) being the least erodible. NT (0.43) was the most effective treatment for reducing SE, while SCM (0.41) enhanced soil fertility, controlled SE, and mitigated machinery-induced soil compaction risks. CM (0.38) maintained soil stability without improving the soil nutrient storage, while ST (0.33) improved the soil stability such as alleviating the soil hardening caused by CM. The results provide reference parameter values for selecting appropriate tillage methods to decrease soil degradation and erosion while enhancing the soil productivity in a red soil hilly region.

To understand the distribution of the soil microbial community in natural walnut orchards at different altitude gradients (3000–3500 m) and to reveal the mechanism of the soil microbial activity in natural walnut orchards adapting to high-altitude environments, soil samples from four groups of natural walnut orchards in Gyaca County, southeast Tibet, were studied. Illumina MiSeq sequencing technology was used to analyze the community composition and diversity of soil bacteria and fungi and their responses to the altitudes. The alpha diversity results showed that the vertical distribution pattern of the fungal community was more obvious than that of the bacterial community and the bacterial community diversity first increased (~3364 m) and then decreased with altitude. The number of amplicon sequence variants (ASVs) in the soil bacterial community was significantly higher than that in the fungal community, but soil bacterial and fungal communities in walnut orchards at different altitudes exhibited both inheritance and uniqueness. At the phylum level, the dominant bacterial phyla at different altitudes were Actinobacteria, Acidobacteria, Proteobacteria, and Chloroflexi (relative abundances > 10.0% in each treatment). With the increase in altitude, the relative abundance of Actinobacteria increased gradually while that of Acidobacteria and Proteobacteria decreased gradually. The dominant fungal phyla were Ascomycota, Basidiomycota, and Mortierellomycota (relative abundances >5.0% in each treatment). With the increase in altitude, the relative abundance of Ascomycota increased significantly. At the genus level, the number of dominant bacteria and fungi in the soil decreased gradually with increased altitude and showed anisotropic distribution characteristics. The relative abundances of Actinobacteria among the bacterial phyla—and Olpidiomycota and Zoopagomycota among the fungal phyla—were positively correlated with the altitude (p < 0.05). Most dominant bacterial and fungal phyla were highly significantly (p < 0.01) or significantly (p < 0.05) negatively correlated with the altitude. Soil nitrogen and phosphorus availabilities are the main limiting factors of microbial community diversity. Therefore, altitude caused changes in soil physicochemical properties which directly or indirectly affected the composition and diversity of soil bacterial and fungal communities, and our study provides a theoretical basis for the altitudinal pattern and succession changes in soil microbial communities in the natural walnut orchards of southeast Tibet.

Walnut trees on the Tibetan Plateau have sustainable and nutritious characteristics. They grow naturally without the use of any chemical fertilizers and pesticides. Therefore, the soil fertility of walnut orchards is a key factor influencing walnut yield and quality. This experiment measured and analyzed the basic soil nutrient content, microbial biomass carbon (MBC), nitrogen (MBN), phosphorus (MBP), and leaf nutrient indicators in five types of representative walnut orchard soil parent materials in Gyaca County to clarify the fertility characteristics of different soil types and their correlations with walnut nutrients, providing a basis for the sustainable development of the plateau walnut industry. The results showed that there were significant differences in soil organic matter (SOM), total nitrogen (TN), available nutrient contents, and microbial activity among soil types with different parent materials. The comprehensive representation of available nutrient levels in the soils is as follows: gray-cinnamon soil and calcareous alluvial soil > mountain meadow soil > plateau prairie soil and brush prairie soil. The average MBC, MBN, and MBP contents in the five soils were 368.8 mg/kg, 28.4 mg/kg, and 23.6 mg/kg, respectively, and the content in the topsoil (0–20 cm) was higher than that of the subsoil (20–40 cm). The total nitrogen (PN), total phosphorus (PP), and total potassium (PK) contents in walnut leaves were found to be closely related to the soil physicochemical properties and microbial activity, among which five indicators, including soil TN, available nitrogen (AN), SOM, MBC, and slowly available potassium (SAK), had the greatest impact on leaf nutrient levels. These findings are valuable for the future management and development of walnut orchards. Therefore, for different soil parent materials and soil types, improving the soil fertility and actively applying nitrogen-rich organic fertilizers should be prioritized to improve the yield and quality of Tibetan Plateau walnuts.

Unraveling the kinetics and mechanisms of K-bearing mineral dissolution in the presence of organic acids on the nanometer scale is important for understanding the effectiveness of organic acids present in most soil environments. Herein, batch experiments were used coupled with atomic force microscopy (AFM) to image the surface dissolution and alteration of the micro- and nano-structure of biotite in pH 4.0 aqueous (H₂O), citric acid (CA), and citric acid with sodium chloride (CA + NaCl) solutions. We directly measured the release of potassium (K), aluminum (Al), and silicon (Si) from biotite into solution and observed the alteration of biotite at room temperature (25 °C) during long reaction times (0–168 h). In the acidic solution, biotite dissolved slowly, but the K, Si and Al release rates could be increased by adding citric acid and salt. The AFM observation indicated that the alteration of biotite was a coupled dissolution–precipitation process. An amorphous Si-bearing coating was deposited on the (001) surface or edge sites in the acidic environment after long time dissolution. Bumps or bulges on the (001) surface accelerated the stripping of the biotite segment from the surface after 96 h of reaction in citrate solutions. Na⁺–K⁺ ion exchange occurred in the biotite interlayer resulting in swellings and cracks on the biotite (001) surface and eventually forming Na-bearing hydrated mica. These observations may contribute to resolving the previously unrecognized interaction of organic acids and K-bearing minerals in a more complex soil system.