The present study is aimed to test the effectiveness and the reproducibility of the acid pre-treatment of sewage sludge to suppress the methanogenic bacteria activity, in order to increase the hydrogen forming bacteria activity, mainly Clostridium species. The treated sludge has been tested on glucose reach medium under mesophilic conditions (35°C), in batch mode to quantify the biological fermentative hydrogen production. In the whole series of experiments, the main components of biogas are hydrogen (52–60%) and carbon dioxide (40–48%); no methane and hydrogen sulphide were present in it. The rate of biogas production reached a maximum of 75 ml/lh. An overall mean hydrogen conversion efficiency was 11.20% on the assumption of maximum of 3 mol H2/mol glucose. Clostridium spp. multiplied ten times after 10 h of fermentation and over that thousand times at the end of fermentation.

In the field of responsive shading systems, the use of photobioreactors (PBRs) containing microalgae seems to be a promising technology. Within this framework, this paper presents a case study where a PBR was specifically conceived as a shading system for an external workspace located on an open terrace of the State Library of Queensland (SLQ) in Brisbane. The proposed shading system makes use of PBRs as translucent screening elements, capable of regulating natural light to provide adequate visual comfort for a multipurpose workspace, despite exposure of the site to direct sunlight and fluctuating weather conditions. The microalgae (Scenedesmus Obliquus) cultivated within the shading elements contribute to improving the environment by bio-sequestrating CO2 and producing oxygen. They are also a valuable raw material containing bioactive compounds with various applications including nutraceutical products, livestock feed, and biofuel synthesis. In addition, their green pigmentation is visually engaging and allows for the filtration of solar radiation. The paper focuses on analyzing the daylight performance in the outdoor workplace under the PBR shade. The biomass density of the PBR is adjustable across a wide range of light transmittances (10%-80%) to counter the highly variable outdoor conditions. For different Tv values, illuminance values were calculated using the simulation tool DIVA-for-Rhino and then analyzed as both point-in-time illuminance in June, September and December, and through climate-based daylight metrics such as the Daylight Autonomy and the Useful Daylight Illuminance. As a further step, the daylighting performances were compared to two other climates (Turin, Italy, and Dubai, United Arab Emirates), to verify which setting of the PBR may be suitable for different boundary conditions, thus making the system more widely applicable. The results in Brisbane showed that for clear sky conditions, higher levels of biomass saturation are needed (Tv < 20%) to remain in the 300-3000 lx 'optimal' illuminance comfort range, with lower concentrations (Tv < 30%) to remain within the 300-6000 lx 'acceptable' illuminance comfort range. Differently, in overcast sky conditions, the optimal range of illuminance is easily obtained with reduced levels of biomass saturation (Tv < 80%).