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Transcriptional regulation is involved in responding to cadmium (Cd) stress in plants. However, the molecular mechanisms of Cd stress responses regulated by transcription factors remain largely unknown in plants. In this study, a rice (Oryza sativa) NAC (no apical meristem [NAM]; Arabidopsis transcription activation factor [ATAF]; cup-shaped cotyledon [CUC]-related) family transcription factor, OsNAC300, was isolated and functionally characterized for its involvement in Cd stress responses and tolerance. OsNAC300 was localized to the nucleus. OsNAC300 was mainly expressed in roots and significantly induced by Cd treatment. Knockout of OsNAC300 resulted in increased sensitivity to Cd stress, while its overexpression lines enhanced tolerance to Cd stress. RNA-Seq analysis revealed that the mutant is impaired in regulating some important genes that were responsive to Cd stress in wild-type rice, such as the pathogenesis-related genes 10a (OsPR10a), OsPR10b, chalcone synthase 1 (OsCHS1), and several others, which was validated by reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis. Moreover, chromatin immunoprecipitation-qPCR assays and luciferase reporter assays demonstrated that OsNAC300 directly binds to the promoters of OsPR10a, OsPR10b, and OsCHS1 and activates their transcription. Overall, OsNAC300 is an important regulatory factor in Cd stress responses and tolerance in rice.
Shubao Hu; Kamran Iqbal Shinwari; Yuxinrui Song; Jixing Xia; Heng Xu; Binbin Du; Le Luo; Luqing Zheng. OsNAC300 Positively Regulates Cadmium Stress Responses and Tolerance in Rice Roots. Agronomy 2021, 11, 95 .
AMA StyleShubao Hu, Kamran Iqbal Shinwari, Yuxinrui Song, Jixing Xia, Heng Xu, Binbin Du, Le Luo, Luqing Zheng. OsNAC300 Positively Regulates Cadmium Stress Responses and Tolerance in Rice Roots. Agronomy. 2021; 11 (1):95.
Chicago/Turabian StyleShubao Hu; Kamran Iqbal Shinwari; Yuxinrui Song; Jixing Xia; Heng Xu; Binbin Du; Le Luo; Luqing Zheng. 2021. "OsNAC300 Positively Regulates Cadmium Stress Responses and Tolerance in Rice Roots." Agronomy 11, no. 1: 95.
Protein diets are well known for body maintenance and weight loss.
Muzahir Hussain; Muhammad Umair Ijaz; Muhammad Ijaz Ahmad; Iftikhar Ali Khan; Sarfaraz Ahmed Brohi; Abid Ullah Shah; Kamran Iqbal Shinwari; Di Zhao; Xinglian Xu; Guanghong Zhou; Chunbao Li. Meat proteins in a high-fat diet have a substantial impact on intestinal barriers through mucus layer and tight junction protein suppression in C57BL/6J mice. Food & Function 2019, 10, 6903 -6914.
AMA StyleMuzahir Hussain, Muhammad Umair Ijaz, Muhammad Ijaz Ahmad, Iftikhar Ali Khan, Sarfaraz Ahmed Brohi, Abid Ullah Shah, Kamran Iqbal Shinwari, Di Zhao, Xinglian Xu, Guanghong Zhou, Chunbao Li. Meat proteins in a high-fat diet have a substantial impact on intestinal barriers through mucus layer and tight junction protein suppression in C57BL/6J mice. Food & Function. 2019; 10 (10):6903-6914.
Chicago/Turabian StyleMuzahir Hussain; Muhammad Umair Ijaz; Muhammad Ijaz Ahmad; Iftikhar Ali Khan; Sarfaraz Ahmed Brohi; Abid Ullah Shah; Kamran Iqbal Shinwari; Di Zhao; Xinglian Xu; Guanghong Zhou; Chunbao Li. 2019. "Meat proteins in a high-fat diet have a substantial impact on intestinal barriers through mucus layer and tight junction protein suppression in C57BL/6J mice." Food & Function 10, no. 10: 6903-6914.
Cadmium (Cd+2) is a highly toxic metal, which significantly alters different biochemical and metabolic processes in plants. Massive amounts of Cd+2 is being released into the environment by different anthropogenic activities. In the present study, plant growth promoting activities of bacterial strain Bacillus cereus was evaluated under Cd+2 stress in two rice cultivars Basmati-385 and Shaheen Basmati. Cd+2 stress significantly decreased plant growth and biomass production in both cultivars. However, with the inoculation of B. cereus under Cd+2 treatments, reduced Cd+2 uptake and increased antioxidant enzymes activities in rice cultivars lead to enhanced plant growth, biomass production, photosynthetic pigments, micronutrients, and lowered electrolytes leakage. This study suggests that B. cereus has the ability to alleviating Cd toxicity and increased phytoremediation efficiency of rice seedling under Cd stress.
Mehmood Jan; Gulmeena Shah; Sadaf Masood; Kamran Iqbal Shinwari; Rashida Hameed; E. S. Rha; Muhammad Jamil. Bacillus Cereus Enhanced Phytoremediation Ability of Rice Seedlings under Cadmium Toxicity. BioMed Research International 2019, 2019, 1 -12.
AMA StyleMehmood Jan, Gulmeena Shah, Sadaf Masood, Kamran Iqbal Shinwari, Rashida Hameed, E. S. Rha, Muhammad Jamil. Bacillus Cereus Enhanced Phytoremediation Ability of Rice Seedlings under Cadmium Toxicity. BioMed Research International. 2019; 2019 ():1-12.
Chicago/Turabian StyleMehmood Jan; Gulmeena Shah; Sadaf Masood; Kamran Iqbal Shinwari; Rashida Hameed; E. S. Rha; Muhammad Jamil. 2019. "Bacillus Cereus Enhanced Phytoremediation Ability of Rice Seedlings under Cadmium Toxicity." BioMed Research International 2019, no. : 1-12.
The uptake and transport of iron (Fe) in plants are both important for plant growth and human health. However, little is known about the mechanism of Fe transport in plants, especially for crops. In the present study, the function of yellow stripe-like 13 (YSL13) in rice was analyzed. OsYSL13 was highly expressed in leaves, especially in leaf blades, whereas its expression was induced by Fe deficiency both in roots and shoots. Furthermore, the expression level of OsYSL13 was higher in older leaves than that in younger leaves. OsYSL13 was located in the plasma membrane. Metal measurement revealed that Fe concentrations were lower in the youngest leaf and higher in the older leaves of the osysl13 mutant under both Fe sufficiency and deficiency conditions, compared with the wild type and two complementation lines. Moreover, the Fe concentrations in the brown rice and seeds of the osysl13 mutant were also reduced. Opposite results were found in OsYSL13 overexpression lines. These results suggest that OsYSL13 is involved in Fe distribution in rice.
Chang Zhang; Kamran Iqbal Shinwari; Le Luo; Luqing Zheng. OsYSL13 Is Involved in Iron Distribution in Rice. International Journal of Molecular Sciences 2018, 19, 3537 .
AMA StyleChang Zhang, Kamran Iqbal Shinwari, Le Luo, Luqing Zheng. OsYSL13 Is Involved in Iron Distribution in Rice. International Journal of Molecular Sciences. 2018; 19 (11):3537.
Chicago/Turabian StyleChang Zhang; Kamran Iqbal Shinwari; Le Luo; Luqing Zheng. 2018. "OsYSL13 Is Involved in Iron Distribution in Rice." International Journal of Molecular Sciences 19, no. 11: 3537.
Cadmium (Cd) is one of the most toxic heavy metals. Cd tolerance ability differs among varieties in plants, but the underlying molecular mechanisms remain largely unknown. In this study, we identified genes that are involved in Cd stress responses and different Cd tolerances of two V. sativa varieties (Cd-tolerant variety (L3) and Cd-sensitive variety (ZM)). Transcriptomic analysis using Illumina pair-end sequencing was carried out on root tissues of L3 and ZM grown with 5 μM and 50 μM of Cd treatments. A de novo assembled V. sativa transcriptome was generated. Differentially expressed genes (DEGs) were assigned to Gene Ontology (GO) functions and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and enrichment analysis was performed. The expression of selected DEGs were confirmed by quantitative reverse transcription–polymerase chain reaction (qRT-PCR). A total of 49,062 sequences were identified as unigenes. In the 5 μM Cd treatment, 69 and 28 differentially expressed unigenes were found as compared with the control in the L3 and ZM respectively, while in the 50 μM Cd treatment, 1036 and 335 differentially expressed unigenes were found in comparison with the control in L3 and ZM. Pathway enrichment analysis suggested that genes related to the cell wall, stress response, the glutathione pathway, metal transporters, and transcription factors are commonly up-regulated in response to Cd stress in both varieties. However, the expression of metal transporter genes and transcription factor genes showed significant differential responses to Cd stress. In addition to the regulation of transcription by transcription factors, metal transporters play a vital role in controlling the different Cd tolerances of V. sativa varieties L3 and ZM.
Haiyun Rui; Xingxing Zhang; Kamran Shinwari; Luqing Zheng; Zhenguo Shen. Comparative transcriptomic analysis of two Vicia sativa L. varieties with contrasting responses to cadmium stress reveals the important role of metal transporters in cadmium tolerance. Plant and Soil 2017, 423, 241 -255.
AMA StyleHaiyun Rui, Xingxing Zhang, Kamran Shinwari, Luqing Zheng, Zhenguo Shen. Comparative transcriptomic analysis of two Vicia sativa L. varieties with contrasting responses to cadmium stress reveals the important role of metal transporters in cadmium tolerance. Plant and Soil. 2017; 423 (1-2):241-255.
Chicago/Turabian StyleHaiyun Rui; Xingxing Zhang; Kamran Shinwari; Luqing Zheng; Zhenguo Shen. 2017. "Comparative transcriptomic analysis of two Vicia sativa L. varieties with contrasting responses to cadmium stress reveals the important role of metal transporters in cadmium tolerance." Plant and Soil 423, no. 1-2: 241-255.
With the rapid progress of industrialization, the effects of environmental contamination on plant toxicity, and subsequently on human health, is a growing concern. For example, the heavy metal pollution of soil such as that caused by cadmium (Cd) is a serious threat. Therefore, screening for pollution-safe edible plants is an essential approach for growing plants under heavy metal-contaminated soils. In the current study, 35 Chinese cabbage (Brassica pekinensis L.) cultivars were selected with the aim of screening for Cd-safe cultivars (CSCs), analyzing their safety, and exploring the mechanism of Cd accumulation. Our field-culture experiments revealed that the Cd content in the edible parts of the cultivars were varied and were determined to possibly be CSCs. Hydroponics experiments were used to simulate six different degrees of soil contamination (high and low Cd concentrations) on possible CSCs. The results indicated a significant difference (p < 0.05) in Cd concentration in the cultivars, and verified the safety of these possible CSCs. The analyses of the transport coefficient and expression levels showed that the differences in Cd accumulation among the Chinese cabbage cultivars were related to the expression of genes involved in absorption and transport rather than a root-to-shoot translocation limitation.
Jingjie Wang; Nan Yu; Guangmao Mu; Kamran Iqbal Shinwari; Zhenguo Shen; Luqing Zheng. Screening for Cd-Safe Cultivars of Chinese Cabbage and a Preliminary Study on the Mechanisms of Cd Accumulation. International Journal of Environmental Research and Public Health 2017, 14, 395 .
AMA StyleJingjie Wang, Nan Yu, Guangmao Mu, Kamran Iqbal Shinwari, Zhenguo Shen, Luqing Zheng. Screening for Cd-Safe Cultivars of Chinese Cabbage and a Preliminary Study on the Mechanisms of Cd Accumulation. International Journal of Environmental Research and Public Health. 2017; 14 (4):395.
Chicago/Turabian StyleJingjie Wang; Nan Yu; Guangmao Mu; Kamran Iqbal Shinwari; Zhenguo Shen; Luqing Zheng. 2017. "Screening for Cd-Safe Cultivars of Chinese Cabbage and a Preliminary Study on the Mechanisms of Cd Accumulation." International Journal of Environmental Research and Public Health 14, no. 4: 395.
Ijaz Malook; Gulmeena Shah; Mehmood Jan; Kamran Iqbal Shinwari; M. Mudasar Aslam; Shafiq Ur Rehman; Muhammad Jamil. Smoke Priming Regulates Growth and the Expression of Myeloblastosis and Zinc-Finger Genes in Rice under Salt Stress. Arabian Journal for Science and Engineering 2017, 42, 2207 -2215.
AMA StyleIjaz Malook, Gulmeena Shah, Mehmood Jan, Kamran Iqbal Shinwari, M. Mudasar Aslam, Shafiq Ur Rehman, Muhammad Jamil. Smoke Priming Regulates Growth and the Expression of Myeloblastosis and Zinc-Finger Genes in Rice under Salt Stress. Arabian Journal for Science and Engineering. 2017; 42 (6):2207-2215.
Chicago/Turabian StyleIjaz Malook; Gulmeena Shah; Mehmood Jan; Kamran Iqbal Shinwari; M. Mudasar Aslam; Shafiq Ur Rehman; Muhammad Jamil. 2017. "Smoke Priming Regulates Growth and the Expression of Myeloblastosis and Zinc-Finger Genes in Rice under Salt Stress." Arabian Journal for Science and Engineering 42, no. 6: 2207-2215.
Development of fingerprints based on DNA markers is necessary for proper identification and standardization of plant species. These techniques are widely used to develop an unquestionable method of plant identification to protect the patents and quality control for industry. In this study, fifteen commercially important medicinal plants of Pakistan were collected from botanical garden of Qarshi Industries (Pvt.) Ltd, Pakistan. The objective was to optimize the extraction of genomic DNA for use in a PCR-based random amplified polymorphic DNA marker approach. The initial protocol used 60 decamers to amplify scorable amplicons; only nine markers produced significant bands in genomic DNA of medicinal plants. These markers generated 51 bands ranging between 250 and 1600 bp. The most important property of genomic markers is polymorphism to enable specific identification; all the used markers showed 100% polymorphism across 15 different plants. Further, six decamers amplified specific bands to reliably identify 8 species. The amplified bands were arranged in a binary matrix and analyzed by DNAMAN version 5.2.2 statistical software. A homology tree was constructed using binary data for nine markers, and four major clusters/clades were observed. The Rose, Mentha and Stevia accessions had shown clear clustering and grouped in major clusters/clads I, II and III respectively. Sixty decamers amplified 51 polymorphic loci in the genomes of 15 commercially valuable accessions. Moreover clear phylogenetic construction was observed in the generation of homolog tree. This protocol could therefore be useful to provide a baseline to authenticate, identify and perform phylogenetic analysis of important medicinal plants used in the Pakistani herbal medicine industry.
Waqar Ahmad; Khushi Muhammad; Altaf Hussain; Habib Ahmad; Khalid Kahn; Iqbal Ahmed Qarshi; Kamran Iqbal Shinwari; Muhammad Shahid Nadeem; Youxiong Que; Ayaz Khan; Javed Iqbal. DNA Fingerprinting of Essential Commercialized Medicinal Plants from Pakistan. American Journal of Plant Sciences 2017, 08, 2119 -2132.
AMA StyleWaqar Ahmad, Khushi Muhammad, Altaf Hussain, Habib Ahmad, Khalid Kahn, Iqbal Ahmed Qarshi, Kamran Iqbal Shinwari, Muhammad Shahid Nadeem, Youxiong Que, Ayaz Khan, Javed Iqbal. DNA Fingerprinting of Essential Commercialized Medicinal Plants from Pakistan. American Journal of Plant Sciences. 2017; 08 (09):2119-2132.
Chicago/Turabian StyleWaqar Ahmad; Khushi Muhammad; Altaf Hussain; Habib Ahmad; Khalid Kahn; Iqbal Ahmed Qarshi; Kamran Iqbal Shinwari; Muhammad Shahid Nadeem; Youxiong Que; Ayaz Khan; Javed Iqbal. 2017. "DNA Fingerprinting of Essential Commercialized Medicinal Plants from Pakistan." American Journal of Plant Sciences 08, no. 09: 2119-2132.