The need for compatible materials for the preservation of cultural heritage has resulted in the revival of lime-based mortar technology and other applications. This work investigates the cohesion and integrity of lime mortars added with fresh mucilage extracted from five plants and evaluates their bioreceptivity for long-term durability. Specimens of lime mortars added with 2.5% of fresh mucilage extracted from Aloe vera, Cylindropuntia californica, Opuntia engelmannii, Opuntia ficus-indica and Salvia hispanica mucilages were analyzed for color change (colorimetry), cohesion (ultrasound measurements), integrity (X-rays) and bioreceptivity (microbiological tests).The internal structure of the specimens added with Cacti mucilages shows better compactness, and no color change was noticed in the bio-mortars also after aging. The bioreceptivity response of mortars inoculated with bacteria, fungi and a photosynthetic biofilm was quite different. Specimens added with Aloe and Cylindropuntia mucilages showed a higher extent of bioreceptivity than the control; the specimens of bio-mortars added with Opuntia engelmannii, Opuntia ficus-indica and Salvia hispanica mucilages did not appear, up to threemonths after the contamination, any microbial growth. These results indicate that the addition of mucilage improves the mortar qualities, but the choice of the plant mucilage must be carefully evaluated since it can be responsible for changes in the bioreceptivity of the mortar.