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Methane emissions from ruminants are a major contributor to agricultural greenhouse gas emissions. Thus, eight different forage species were combined in binary mixtures with Lolium perenne in increasing proportions, in vitro, to determine their methane reduction potential in ruminants. Species were sampled in two consecutive years where possible. The aims were: a) to determine if mixtures with specific forages, particularly those rich in plant specialized metabolites (PSM), can reduce methane emissions compared to ryegrass monocultures, b) to identify whether there is a linear-dose effect relationship in methane emissions from the legume or herb addition, and c) whether these effects are maintained across sampling years. Results showed that all dicot species studied, including the non-tannin-containing species, reduced methane production. The tannin-rich species, Sanguisorba minor and Lotus pedunculatus, showed the greatest methane reduction potential of up to 33%. Due to concomitant reductions in the forage digestibility, Cichorium intybus yielded the lowest methane emissions per digestible forage unit. Contrary to total gas production, methane production was less predictable, with a tendency for the lowest methane production being obtained with a 67.5% share of the legume or herb partner species. Thus, linear increments in the partner species share did not result in linear changes in methane concentration. The methane reduction potential differed across sampling years, but the species ranking in methane concentration was stable.
Cecilia Loza; Supriya Verma; Siegfried Wolffram; Andreas Susenbeth; Ralf Blank; Friedhelm Taube; Ralf Loges; Mario Hasler; Christof Kluß; Carsten Malisch. Assessing the Potential of Diverse Forage Mixtures to Reduce Enteric Methane Emissions In Vitro. Animals 2021, 11, 1126 .
AMA StyleCecilia Loza, Supriya Verma, Siegfried Wolffram, Andreas Susenbeth, Ralf Blank, Friedhelm Taube, Ralf Loges, Mario Hasler, Christof Kluß, Carsten Malisch. Assessing the Potential of Diverse Forage Mixtures to Reduce Enteric Methane Emissions In Vitro. Animals. 2021; 11 (4):1126.
Chicago/Turabian StyleCecilia Loza; Supriya Verma; Siegfried Wolffram; Andreas Susenbeth; Ralf Blank; Friedhelm Taube; Ralf Loges; Mario Hasler; Christof Kluß; Carsten Malisch. 2021. "Assessing the Potential of Diverse Forage Mixtures to Reduce Enteric Methane Emissions In Vitro." Animals 11, no. 4: 1126.
In recent years, several secondary plant metabolites have been identified that possess antimethanogenic properties. Tannin-rich forages have the potential to reduce methane emissions in ruminants while also increasing their nutrient use efficiency and promoting overall animal health. However, results have been highly inconclusive to date, with their antimethanogenic potential and effects on both animal performance and nutrition being highly variable even within a plant species. This variability is attributed to the structural characteristics of the tannins, many of which have been linked to an increased antimethanogenic potential. However, these characteristics are seldom considered in ruminant nutrition studies—often because the analytical techniques are inadequate to identify tannin structure and the focus is mostly on total tannin concentrations. Hence, in this article, we (i) review previous research that illustrate the variability of the antimethanogenic potential of forages; (ii) identify the source of inconsistencies behind these results; and (iii) discuss how these could be optimized to generate comparable and repeatable results. By adhering to this roadmap, we propose that there are clear links between plant metabolome and physiology and their antimethanogenic potential that can be established with the ultimate goal of improving the sustainable intensification of livestock.
Supriya Verma; Friedhelm Taube; Carsten Malisch. Examining the Variables Leading to Apparent Incongruity between Antimethanogenic Potential of Tannins and Their Observed Effects in Ruminants—A Review. Sustainability 2021, 13, 2743 .
AMA StyleSupriya Verma, Friedhelm Taube, Carsten Malisch. Examining the Variables Leading to Apparent Incongruity between Antimethanogenic Potential of Tannins and Their Observed Effects in Ruminants—A Review. Sustainability. 2021; 13 (5):2743.
Chicago/Turabian StyleSupriya Verma; Friedhelm Taube; Carsten Malisch. 2021. "Examining the Variables Leading to Apparent Incongruity between Antimethanogenic Potential of Tannins and Their Observed Effects in Ruminants—A Review." Sustainability 13, no. 5: 2743.
Biological Nitrification Inhibition (BNI) of Brachiaria humidicola has been attributed to nitrification‐inhibiting fusicoccanes, most prominently 3‐epi‐brachialactone. However, its release mechanism from B. humidicola roots remains elusive. Two hydroponic experiments were performed to investigate the role of rhizosphere pH and nutritional N form in regulating 3‐epi‐brachialactone release by B. humidicola and verify the underlying release pathway. Low rhizosphere pH and NH4+ nutrition promoted 3‐epi‐brachialactone exudation. However, the substitution of NH4+ by K+ revealed that the NH4+ effect was not founded in a direct physiological response to the N form but was related to the cation‐anion balance during nutrient uptake. Release of 3‐epi‐brachialactone correlated with the transmembrane proton gradient ΔpH and NH4+ uptake (R2 = 0.92 for high ~ 6.8 and R2 = 0.84 for low ~ 4.2 trap solution pH). This corroborated the release of 3‐epi‐brachialactone through secondary transport, with the proton motive force (ΔP) defining transport rates across the plasma membrane. It was concluded that 3‐epi‐brachialactone release cannot be conceptualized as a regulated response to soil pH or NH4+ availability, but merely as the result of associated changes in ΔP.
Konrad Egenolf; Supriya Verma; Jochen Schöne; Iris Klaiber; Jacobo Arango; Georg Cadisch; Günter Neumann; Frank Rasche. Rhizosphere pH and cation‐anion balance determine the exudation of nitrification inhibitor 3‐ epi ‐brachialactone suggesting release via secondary transport. Physiologia Plantarum 2020, 172, 116 -123.
AMA StyleKonrad Egenolf, Supriya Verma, Jochen Schöne, Iris Klaiber, Jacobo Arango, Georg Cadisch, Günter Neumann, Frank Rasche. Rhizosphere pH and cation‐anion balance determine the exudation of nitrification inhibitor 3‐ epi ‐brachialactone suggesting release via secondary transport. Physiologia Plantarum. 2020; 172 (1):116-123.
Chicago/Turabian StyleKonrad Egenolf; Supriya Verma; Jochen Schöne; Iris Klaiber; Jacobo Arango; Georg Cadisch; Günter Neumann; Frank Rasche. 2020. "Rhizosphere pH and cation‐anion balance determine the exudation of nitrification inhibitor 3‐ epi ‐brachialactone suggesting release via secondary transport." Physiologia Plantarum 172, no. 1: 116-123.