Prof. Luis Antonio Tortajada-Genaro
Dept. Chemistry, Universitat Politècnica de València, Valencia, Spain
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Academic Career Length
27Years (2000-Present)
(Calculated once Scilit publication data is synced: current year − year of first publication + 1)
Representative Publications
View All Publications1 February 2012 in Atmospheric Environment
Journal Article
EBEsther Borrás
Esther Borrás
Luis Antonio Tortajada-Genaro1 February 2014 in Analytica Chimica Acta
Journal Article
SSSara Santiago-Felipe
Sara Santiago-FelipeLuis Antonio Tortajada-GenaroRPR. Puchades
R. Puchades
Ángel MaquieiraPolymerase chain reaction in conjunction with enzyme-linked immunosorbent assay (PCR-ELISA) is a well-established technique that provides a suitable rapid, sensitive, and selective method for a broad range of applications. However, the need for precise rapid temperature cycling of PCR is an important drawback that can be overcome by employing isothermal amplification reactions such as recombinase polymerase amplification (RPA). The RPA-ELISA combination is proposed for amplification at a low, constant temperature (40°C) in a short time (40 min), for the hybridisation of labelled products to specific 5'-biotinylated probes/streptavidin in coated microtiter plates at room temperature, and for detection by colorimetric immunoassay. RPA-ELISA was applied to screen common safety threats in foodstuffs, such as allergens (hazelnut, peanut, soybean, tomato, and maize), genetically modified organisms (P35S and TNOS), pathogenic bacteria (Salmonella sp. and Cronobacter sp.), and fungi (Fusarium sp.). Satisfactory sensitivity and reproducibility results were achieved for all the targets. The RPA-ELISA technique does away with thermocycling and provides a suitable sensitive, specific, and cost-effective method for routine applications, and proves particularly useful for resource-limited settings.
1 December 2009 in Atmospheric Environment
Journal Article
EBEsther Borrás
Esther Borrás
Luis Antonio Tortajada-GenaroMVMonica Vázquez
Monica Vázquez
BZBarbara Zielinska
Barbara Zielinska
1 August 2011 in Analytica Chimica Acta
Journal Article
EBE. Borrás
E. Borrás
PSP. Sánchez
P. Sánchez
AMAmalia Muñoz
Amalia MuñozLuis Antonio Tortajada-Genaro12 June 2009 in Analytical Chemistry
Journal Article
Sergi MoraisLuis Antonio Tortajada-GenaroTATania Arnandis-Chover
Tania Arnandis-Chover
RPRosa Puchades
Rosa PuchadesÁngel Maquieira1 May 2015 in Food Chemistry
Journal Article
SSSara Santiago-Felipe
Sara Santiago-FelipeLuis Antonio Tortajada-GenaroSergi MoraisRPRosa Puchades
Rosa PuchadesÁngel MaquieiraAbout Me
L.A.
Tortajada-Genaro earned his Ph.D. in Chemistry from the University of Valencia
(2002, Excellence Award). He held research positions at the University of
Plymouth, CEAM Foundation, and Clinical Hospital of Valencia, and was a
visiting researcher at KTH Royal Institute of Technology. Currently, he is a Full
Professor in the Chemistry Department at the Polytechnic University of
Valencia. He is a member of the Interuniversity Research Institute for
Molecular Recognition and Technological Development (IDM), RSEQ, and SEQA. His
research focuses on Bioanalytics, specializing in biosensing applied to health,
food quality/safety, and environmental monitoring. He has participated in
regional, national, and European research projects and reviews for over 30
high-impact journals. Dr. Tortajada-Genaro has co-authored 72 scientific
papers, 8 international book chapters, and over 190 conference communications.
He holds 4 patents and has supervised 5 Ph.D. theses and multiple master’s
theses. His recent work focuses on biosensor solutions, IVD test and device
design, and DNA/immunoassays for clinical biomarkers. He has extensive
experience in research management and technology transfer, collaborating across
academia, industry, and healthcare to translate biosensing technologies into
practical diagnostic solutions. Scientific
IDs: ORCID: 0000-0003-4021-5607 | SCOPUS ID: 6602446748