Plant viral diseases are the foremost threat to sustainable agriculture, leading to several billion dollars in losses every year. Many viruses infecting several crops have been described in the literature; however, new infectious viruses are emerging frequently through outbreaks. For the effective treatment and prevention of viral diseases, there is great demand for new techniques that can provide accurate identification on the causative agents. With the advancements in biochemical and molecular biology techniques, several diagnostic methods with improved sensitivity and specificity for the detection of prevalent and/or unknown plant viruses are being continuously developed. Currently, serological and nucleic acid methods are the most widely used for plant viral diagnosis. Nucleic acid-based techniques that amplify target DNA/RNA have been evolved with many variants. However, there is growing interest in developing techniques that can be based in real-time and thus facilitate in-field diagnosis. Next-generation sequencing (NGS)-based innovative methods have shown great potential to detect multiple viruses simultaneously; however, such techniques are in the preliminary stages in plant viral disease diagnostics. This review discusses the recent progress in the use of NGS-based techniques for the detection, diagnosis, and identification of plant viral diseases. New portable devices and technologies that could provide real-time analyses in a relatively short period of time are prime important for in-field diagnostics. Current development and application of such tools and techniques along with their potential limitations in plant virology are likewise discussed in detail.

Traditional fermented foods have been recognized by various communities to be good for health since ancient times. There is a provincial legacy of traditional fermented foods among the ethnic population of North-East India. Fermented bamboo shoots (local name: Tuaither), soybeans (Bekang), and pork fat (Sa-um) are famous in the Mizoram state and represent a primary portion of the daily diet. These foods are prepared using methods based on cultural traditions inherited from previous generations, and prepared using a relatively uncontrolled fermentation process. Analysis of the bacterial diversity in these foods can provide important information regarding the flavor and texture of the final products of fermentation. Unfortunately, studies on the microbial composition and health benefits of such traditional fermented foods have rarely been documented. Therefore, the present study aims to highlight this bacterial diversity, along with the proximate composition of different traditional fermented foods (Tuaither, Bekang and Sa-um) primarily consumed in Mizoram state, India. Samples were collected on three different days of fermentation (3rd, 5th and 7th day), and bacterial diversity analysis was performed using the V3-V4 variable region of 16S rRNA gene with Illumina sequencing. Results revealed differences in the bacterial composition of dominant group members among all of the three food types. Firmicutes (82.72–94.00%), followed by Proteobacteria (4.67–15.01%), were found to dominate to varying degrees in all three of the fermented foods. However, at genus level high variation was observed in bacterial composition among these three different types of fermented foods. Lactobacillus (91.64–77.16%), Staphylococcus (52.00–17.90%), and Clostridium (72.48–55.40%) exhibited the highest relative abundances in the Tuaither, Bekang and Sa-um foods, respectively, in descending order from the 3rd to 7th day of fermentation. A few of the bacterial genera such as Lactobacilli were positively correlated with fermented bamboo shoot samples, and Staphylococcus was positively correlated with protein, carbohydrate and crude fiber content in soybean samples. In general, Tuaither, Bekang and Sa-um exhibited distinct differences in bacterial composition. This variation may be due to differences in the raw materials and/or methods used in the preparation of the different fermented food products. This is the first study to describe the bacterial composition of these traditional fermented foods using high-throughput sequencing techniques, and could help to drive research attention to comprehensive studies on improving understanding of the role of microbial communities in the preparation of traditional foods and their health benefits.

The shifting agriculture (popularly known as ‘Jhum’) in north-eastern Himalayas is plagued by substantial reduction in paddy yield and soil quality. The region’s paddy yield and soil condition may be improved by the use of right integrated nutrient management (INM) technology on the jhum lands. We used 7 treatments replicated thrice in a randomized block design (RBD) to evaluate the soil health and yield of direct-seeded upland paddy (Oryza sativa L.) in this study. The results showed that the combination of inorganic fertilization, organic manure, and bio-fertilizers (T₅) significantly (P < 0.05) increased paddy height, number of tillers hill⁻¹, number of grains panicle⁻¹ and test seed weight. Simultaneously, these combined treatments significantly increased soil organic carbon and available Nitrogen (N), Phosphorus (P) and Potassium (K) contents. All the soil chemical properties except the soil pH significantly (p < 0.05; n = 7) affected various growth and yield parameters of paddy. The INM of organic manure and bio-fertilizers (T₇) significantly enhanced the soil’s biological properties. Thus, INM technology is recommended to enhance paddy yield while sustaining the soil health in upland paddy cultivation.

Background: Replenishing the lost nutrients through the use of various soil amendments is vital in order to maintain soil fertility for sustained productivity in jhumlnd. Integrated Nutrient Management, can boost the upland rice productivity in jhumland by building up the lost soil nutrients. Methods: The study was conducted to evaluate the impact of integrated nutrient management on growth and yield of upland paddy (Oryza sativa L.) in Lai-Lad, Jirang Block of Ri-Bhoi District, Meghalaya, India; during 2019 and 2020. Randomised block design with 12 treatments was applied for the trial with 3 replications. Result: Application of 100% recommended doses of fertilizers + farmyard manure + Azospirillum lipoferum + phosphorus solubilizing bacteria + potassium mobilising bacteria + Glomus + zinc solubilizing bacteria (T10) significantly increased plant growth in both years of cultivation. Grain yield, harvest index and benefit cost ratio of upland paddy were at par among the treatments and were higher in 2nd cropping year. Integrated nutrient management could increase the growth characters, maintain sustained yield and economic profitability of upland paddy in hilly terrains of north-east India, under jhum cultivation.