Expired dairy products are often disposed of due to the potential health hazard they pose to living organisms. Lack of methods to recover valuable components from them are also a reason for manufactures to dispose of the expired dairy products. Milk encompasses several different components with their own functional properties that can be applied in production of food and non-food technical products. This study aims to investigate the novel approach of using liquid biphasic flotation (LBF) method for protein extraction from expired milk products and obtaining the optimal operating conditions for protein extraction. The optimized conditions were found at 80% concentration ethanol as top phase, 150 g/L dipotassium hydrogen phosphate along with 10% (w/v) milk as bottom phase, and a flotation time of 7.5 min. The protein recovery yield and separation efficiency after optimization were 94.97% and 86.289%, respectively. The experiment has been scaled up by 40 times to ensure it can be commercialized, and the protein recovery yield and separation efficiency were found to be 78.92% and 85.62%, respectively. This novel approach gives a chance for expired milk products to be changed from waste to raw materials which is beneficial for the environment and the economy.

Densification of ashy biochar into tablet can enhance the handling and conveyance efficiencies of biochar. It was hypothesized that fertilizer-embedded biochar tablets can slowly release embedded nutrients in synchrony with optimum nutrient uptake by crops. The objectives of this research were to determine the effects of biochar tablets with and without embedded fertilizer on soil chemical properties and nutrient use efficiency of Zea mays (sweet corn). The biochar tablet (BT) was produced by blending a biochar mixture with starch followed by densification using a single punch tablet press whereas the fertilizer embedded biochar tablet (BF) was prepared using the same procedure except that NPK fertilizer was added during blending. A pot experiment with five fertilization treatments including control was carried out in an open field located in Perlis, Malaysia. Co-application of biochar and fertilizer increased soil total carbon, nitrogen, but it reduced soil electrical conductivity (EC). Additionally, the BF significantly increased leaf chlorophyll content, dry root weight, and total plant nutrient use efficiency of sweet corn. The findings suggest that BF can serve as a slow release fertilizer to improve crop nutrient use efficiency. Therefore, embedding fertilizer in biochar tablets is recommended for sweet corn production following a long term field study to confirm the findings of this pot study.

Indole-3-acetic acid (IAA) phytohormone plays an essential role in forming and initiating main, lateral, and adventitious roots in vegetative propagation. Plants are receiving IAA naturally from a diverse group of soil-plant associated rhizobacteria. However, IAA synthesis by rhizobacteria is influenced by abiotic growth conditions. Three indigenous Bacillus isolates were subject to in vitro assay for the effects of abiotic factors (temperature, salinity and pH) on growth and IAA production. All isolates grew well between 25 - 40°C, and only B. megaterium UPMLH3 was capable of synthesising IAA (21.18 µg/ml) at 40°C. All three bacterial growth under saline stress were slightly dropped over control (0% NaCl), but still producing IAA up to 1% NaCl condition. B. cereus UPMLH24 revealed high resistance to salinity up to 5% NaCl. The optimum growth of all three Bacillus spp. was at pH 7. B. cereus UPMLH1 and UPMLH24 discovered higher IAA production in slightly alkaline conditions (pH 8). Each rhizobacterium shows different physiology trait against each abiotic factor. However, the multiple tolerance ability of PGPR against abiotic factors is an indication that its ability to survive under harsh soil and plant environments while delivering benefits to the plant. Thus, B. cereus UPMLH1, B. megaterium UPMLH3 and B. cereus UPMLH24 might serve as potential biofertiliser, enhancing the growth performance of test plants at various environmental conditions.

We were not aware of some errors made in the proofreading phase; therefore, we wish to make the following corrections to the mathematical equations in the text

Trichoderma longibrachiatum rarely reported can cause disease in plants. The present study investigates the pathogenicity of T. longibrachiatum isolate UPMT14 on the red leaf lettuce (Lactuca sativa L.) plants grown in sterile soil under a controlled growth room environment. The fungal isolate was initially characterized morphologically as Trichoderma sp. and was then further characterized by (ITS) region sequencing and BLAST comparison identified as T. longibrachiatum. To observe the response of Trichoderma isolate UPMT14 when imposed on lettuce plants. The injection was made and repeated five times, and then the lettuce growth followed for 36 days. On day 36, the present study found that the red leaf lettuce plants expressed foliar symptoms that began as chlorotic, reduced plant height, reduced leaf length and diameter, wilt, and dried up before it collapsed at day 45 compared to untreated control lettuce plants. Microscopic observation on lettuce roots showed that the Trichoderma spores invading the root system by mass sporulation and spatial competition possibly impaired plant water uptake and eventually caused plant wilting. Therefore, this study indicates that T. longibrachiatum is among the causal agents of wilt disease in the lettuce plant.