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In this work, the chemical compositions of basils oils, including those of lemon basil, white holy basil, Thai basil, tree basil and red holy basil, were analysed. Methyl eugenol was detected in all types of basils. The essential oils of red and white holy basils possessed a comparable ability (~25%) to attract male Oriental fruit fly to the synthesised fruit fly attractant in the laboratory experiment. To control the release of the active ingredients, the white holly basil oil (WBO) was encapsulated with maltodextrin (MD) and gum arabic (GA) by paste method. The essential oil is retained in the wall complex much longer with the addition of MD. The results also revealed that the combination of the MD:GA (25:75) had the highest loading efficiency of the oil (9.40%) as observed by the numerous porous structures by scanning electron microscopy. Fourier-transform infrared spectra of the encapsulated polymer confirmed traces of essential oil functional groups. The field test study advised that WBO-encapsulated products improved fruit fly attractive efficiency by maintaining the release rate of basil essential oil.
Tibet Tangpao; Patcharin Krutmuang; Wilawan Kumpoun; Pensak Jantrawut; Tonapha Pusadee; Ratchadawan Cheewangkoon; Sarana Sommano; Bajaree Chuttong. Encapsulation of Basil Essential Oil by Paste Method and Combined Application with Mechanical Trap for Oriental Fruit Fly Control. Insects 2021, 12, 633 .
AMA StyleTibet Tangpao, Patcharin Krutmuang, Wilawan Kumpoun, Pensak Jantrawut, Tonapha Pusadee, Ratchadawan Cheewangkoon, Sarana Sommano, Bajaree Chuttong. Encapsulation of Basil Essential Oil by Paste Method and Combined Application with Mechanical Trap for Oriental Fruit Fly Control. Insects. 2021; 12 (7):633.
Chicago/Turabian StyleTibet Tangpao; Patcharin Krutmuang; Wilawan Kumpoun; Pensak Jantrawut; Tonapha Pusadee; Ratchadawan Cheewangkoon; Sarana Sommano; Bajaree Chuttong. 2021. "Encapsulation of Basil Essential Oil by Paste Method and Combined Application with Mechanical Trap for Oriental Fruit Fly Control." Insects 12, no. 7: 633.
Bioactive molecules of plant origin play a significant role as defensive agents in different insect species. Chemical compounds in medicinal plants have been an exciting alternative to standard methods of controlling mosquito larvae. The present study evaluates the different solvent extracts of D. hamiltonii for toxicity against three different mosquito larvae. Bioassay revealed that the effect of the methanol extracts increased the larval mortality with increasing concentration. The highest larval mortality was observed in Culex quinquefasciatus with 98.33%, followed by 95 and 90% mortality in Aedes aegypti and Anopheles stephensi, at 24 h exposure. GC-MS analysis of methanol extract of D. hamiltonii showed six major peak compounds. They are benzaldehyde, 2-hydroxy-4-methoxy-(10.35%), dodecanoic acid (11.02%), n-hexadecanoic acid (21.05%), linoleic acid methyl ester (14.20%), oleic acid (21.04%), octadecanoic acid (22.21%). The level of α and β Carboxylesterases gets significantly decreased post-treatment with the methanol extract of D. hamiltonii in a dose-dependent manner.In contrast, glutathione S-transferase (GST) and cytochrome-P450 (CYP450) levels get up-lifted steadily when the dosage gets increased. The ratio of GST level has drastically proclaimed to in Ae. aegypti 0.702 mg/m Lin parallel to Cx. quiquefasciatus (0.656 mg/mL) and An. stephensi (0.812 mg/mL). Cytochrome P450 (CYP450) activity was observed to increase significantly post-treatment with the sub-lethal dosage of methanol extract of D. hamiltonii. Correspondingly, the non-target screening against the aquatic predators reveals that the crude root extracts and their derivatives are ecologically safe and less toxic. Overall, the present research highlights the chemical characterization of crude methanol extracts of D. hamiltonii, their insecticidal activity against the medically challenging pests, and their non-target activity delivers an ecologically safe, and target specific bio-active agents and suitable substitute for chemical pesticides.
Palanisamy Prakash; Ekambaram Gayathiri; Rengarajan Manivasagaperumal; Patcharin Krutmuang. Biological Activity of Root Extract Decalepis hamiltonii (Wight & Arn) against Three Mosquito Vectors and Their Non-Toxicity against the Mosquito Predators. Agronomy 2021, 11, 1267 .
AMA StylePalanisamy Prakash, Ekambaram Gayathiri, Rengarajan Manivasagaperumal, Patcharin Krutmuang. Biological Activity of Root Extract Decalepis hamiltonii (Wight & Arn) against Three Mosquito Vectors and Their Non-Toxicity against the Mosquito Predators. Agronomy. 2021; 11 (7):1267.
Chicago/Turabian StylePalanisamy Prakash; Ekambaram Gayathiri; Rengarajan Manivasagaperumal; Patcharin Krutmuang. 2021. "Biological Activity of Root Extract Decalepis hamiltonii (Wight & Arn) against Three Mosquito Vectors and Their Non-Toxicity against the Mosquito Predators." Agronomy 11, no. 7: 1267.
The present research investigated the chemical characterization and insecticidal activity of n-Hexane extracts of Epaltes divaricata (NH-EDx) along with their chief derivatives n-Hexadecanoic acid (n-HDa) and n-Octadecanoic acid (n-ODa) against the dengue vector Aedes aegypti and lepidopteran pest Spodoptera litura. Chemical screening of NH-EDx through GC–MS analysis delivered nine major derivatives, and the maximum peak area percentage was observed in n-Hexadecanoic acid (14.63%) followed by n-Octadecadienoic acid (6.73%). The larvicidal activity of NH-EDx (1000 ppm), n-HDa (5 ppm), and n-ODa (5 ppm) against the A. aegypti and S. litura larvae showed significant mortality rate in a dose-dependent way across all the instars. The larvicidal activity was profound in the A. aegypti as compared to the S. litura across all the larval instars. The sublethal dosages of NH-EDx (500 ppm), n-HDa (2.5 ppm), and n-ODa (2.5 ppm) also showed alterations in the larval/pupal durations and adult longevity in both the insect pests. The enzyme activity revealed that the α- and β-carboxylesterase levels were decreased significantly in both the insect pests, whereas the levels of GST and CYP450 uplifted in a dose-dependent manner of NH-EDx, n-HDa, and n-ODa. Correspondingly, midgut tissues such as the epithelial layer (EL), gut lumen (GL), peritrophic matrix (Pm), and brush border membrane (BBM) were significantly altered in their morphology across both A. aegypti and S. litura against the NH-EDx and their bioactive metabolites. NH-EDx and their bioactive metabolites n-HDa and n-ODa showed significant larvicidal, growth retardant, enzyme inhibition, and midgut toxicity effects against two crucial agriculturally and medically challenging insect pest of ecological importance.
Kesavan Amala; Sengodan Karthi; Raja Ganesan; Narayanaswamy Radhakrishnan; Kumaraswamy Srinivasan; Abd Mostafa; Abdullah Al-Ghamdi; Jawaher Alkahtani; Mohamed Elshikh; Sengottayan Senthil-Nathan; Prabhakaran Vasantha-Srinivasan; Patcharin Krutmuang. Bioefficacy of Epaltes divaricata (L.) n-Hexane Extracts and Their Major Metabolites against the Lepidopteran Pests Spodoptera litura (fab.) and Dengue Mosquito Aedes aegypti (Linn.). Molecules 2021, 26, 3695 .
AMA StyleKesavan Amala, Sengodan Karthi, Raja Ganesan, Narayanaswamy Radhakrishnan, Kumaraswamy Srinivasan, Abd Mostafa, Abdullah Al-Ghamdi, Jawaher Alkahtani, Mohamed Elshikh, Sengottayan Senthil-Nathan, Prabhakaran Vasantha-Srinivasan, Patcharin Krutmuang. Bioefficacy of Epaltes divaricata (L.) n-Hexane Extracts and Their Major Metabolites against the Lepidopteran Pests Spodoptera litura (fab.) and Dengue Mosquito Aedes aegypti (Linn.). Molecules. 2021; 26 (12):3695.
Chicago/Turabian StyleKesavan Amala; Sengodan Karthi; Raja Ganesan; Narayanaswamy Radhakrishnan; Kumaraswamy Srinivasan; Abd Mostafa; Abdullah Al-Ghamdi; Jawaher Alkahtani; Mohamed Elshikh; Sengottayan Senthil-Nathan; Prabhakaran Vasantha-Srinivasan; Patcharin Krutmuang. 2021. "Bioefficacy of Epaltes divaricata (L.) n-Hexane Extracts and Their Major Metabolites against the Lepidopteran Pests Spodoptera litura (fab.) and Dengue Mosquito Aedes aegypti (Linn.)." Molecules 26, no. 12: 3695.
The overuse of synthetic pesticides in plant protection strategies has resulted in numerous side effects, including environmental contamination, food staff residues, and a threat to non-target organisms. Several studies have been performed to assess the pesticidal effects of plant-derived essential oils and their components, as partially safe and effective agents, on economically important pests. The essential oils isolated from Satureja species are being used in medicinal, cosmetic, and food industries. Their great potential in pest management is promising, which is related to high amounts of terpenes presented in this genus. This review is focused on the acute and chronic acaricidal, insecticidal, and nematicidal effects of Satureja essential oil and their main components. The effects of eighteen Satureja species are documented, considering lethality, repellency, developmental inhibitory, and adverse effects on the feeding, life cycle, oviposition, and egg hatching. Further, the biochemical impairment, including impairments in esterases, acetylcholinesterase, and cytochrome P450 monooxygenases functions, are also considered. Finally, encapsulation and emulsification methods, based on controlled-release techniques, are suggested to overcome the low persistence and water solubility restrictions of these biopesticides. The present review offers Satureja essential oils and their major components as valuable alternatives to synthetic pesticides in the future of pest management.
Asgar Ebadollahi; Jalal Jalali Sendi; Masumeh Ziaee; Patcharin Krutmuang. Acaricidal, Insecticidal, and Nematicidal Efficiency of Essential Oils Isolated from the Satureja Genus. International Journal of Environmental Research and Public Health 2021, 18, 6050 .
AMA StyleAsgar Ebadollahi, Jalal Jalali Sendi, Masumeh Ziaee, Patcharin Krutmuang. Acaricidal, Insecticidal, and Nematicidal Efficiency of Essential Oils Isolated from the Satureja Genus. International Journal of Environmental Research and Public Health. 2021; 18 (11):6050.
Chicago/Turabian StyleAsgar Ebadollahi; Jalal Jalali Sendi; Masumeh Ziaee; Patcharin Krutmuang. 2021. "Acaricidal, Insecticidal, and Nematicidal Efficiency of Essential Oils Isolated from the Satureja Genus." International Journal of Environmental Research and Public Health 18, no. 11: 6050.
The interleukin-4 (IL-4) and interleukin-4 receptor (IL-4R) are cytokines that are involved in the immune and reproductive systems. This study aimed to verify the polymorphisms in the porcine IL-4 and IL-4R genes and to assess their effects on litter size traits in commercial pigs. Single nucleotide polymorphisms (SNPs) in the porcine IL-4 and IL-4R genes were genotyped by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. A non-coding SNP of IL-4 g.134993898T > C and a non-synonymous SNP of IL-4R c.1577A > T (amino acid change at position 526, Q526L) were found to be segregating in Landrace sows. The IL-4 g.134993898T > C polymorphism was significantly associated with the number of piglets weaned alive (NWA) trait. The IL-4R c.1577A > T polymorphism was significantly associated with the number born alive (NBA) and NWA traits. Moreover, the accumulation of favorable alleles of these two SNP markers revealed significant associations with the NBA, NWA, and mean weight of piglets at weaning (MWW) traits. These findings indicate that the porcine IL-4 and IL-4R genes may contribute to the reproductive traits of pigs and could be used as candidate genes to improve litter size traits in the pig breeding industry.
Worrarak Norseeda; Guisheng Liu; Tawatchai Teltathum; Pantaporn Supakankul; Korawan Sringarm; Watcharapong Naraballobh; Trisadee Khamlor; Siriwadee Chomdej; Korakot Nganvongpanit; Patcharin Krutmuang; Supamit Mekchay. Association of IL-4 and IL-4R Polymorphisms with Litter Size Traits in Pigs. Animals 2021, 11, 1154 .
AMA StyleWorrarak Norseeda, Guisheng Liu, Tawatchai Teltathum, Pantaporn Supakankul, Korawan Sringarm, Watcharapong Naraballobh, Trisadee Khamlor, Siriwadee Chomdej, Korakot Nganvongpanit, Patcharin Krutmuang, Supamit Mekchay. Association of IL-4 and IL-4R Polymorphisms with Litter Size Traits in Pigs. Animals. 2021; 11 (4):1154.
Chicago/Turabian StyleWorrarak Norseeda; Guisheng Liu; Tawatchai Teltathum; Pantaporn Supakankul; Korawan Sringarm; Watcharapong Naraballobh; Trisadee Khamlor; Siriwadee Chomdej; Korakot Nganvongpanit; Patcharin Krutmuang; Supamit Mekchay. 2021. "Association of IL-4 and IL-4R Polymorphisms with Litter Size Traits in Pigs." Animals 11, no. 4: 1154.
European Apis mellifera and Asian Apis cerana honeybees are essential crop pollinators. Microbiome studies can provide complex information on health and fitness of these insects in relation to environmental changes, and plant availability. Amplicon sequencing of variable regions of the 16S rRNA from bacteria and the internally transcribed spacer (ITS) regions from fungi and plants allow identification of the metabiome. These methods provide a tool for monitoring otherwise uncultured microbes isolated from the gut of the honeybees. They also help monitor the composition of the gut fungi and, intriguingly, pollen collected by the insect. Here, we present data from amplicon sequencing of the 16S rRNA from bacteria and ITS2 regions from fungi and plants derived from honeybees collected at various time points from anthropogenic landscapes such as urban areas in Poland, UK, Spain, Greece, and Thailand. We have analysed microbial content of honeybee intestine as well as fungi and pollens. Furthermore, isolated DNA was used as the template for screening pathogens: Nosema apis, N. ceranae, N. bombi, tracheal mite (Acarapis woodi), any organism in the parasitic order Trypanosomatida, including Crithidia spp. (i.e., Crithidia mellificae), neogregarines including Mattesia and Apicystis spp. (i.e., Apicistis bombi). We conclude that differences between samples were mainly influenced by the bacteria, plant pollen and fungi, respectively. Moreover, honeybees feeding on a sugar based diet were more prone to fungal pathogens (Nosema ceranae) and neogregarines. In most samples Nosema sp. and neogregarines parasitized the host bee at the same time. A higher load of fungi, and bacteria groups such as Firmicutes (Lactobacillus); γ -proteobacteria, Neisseriaceae, and other unidentified bacteria was observed for Nosema ceranae and neogregarine infected honeybees. Healthy honeybees had a higher load of plant pollen, and bacteria groups such as: Orbales, Gilliamella, Snodgrassella, and Enterobacteriaceae. Finally, the period when honeybees switch to the winter generation (longer-lived forager honeybees) is the most sensitive to diet perturbations, and hence pathogen attack, for the whole beekeeping season. It is possible that evolutionary adaptation of bees fails to benefit them in the modern anthropomorphised environment.
Aneta Ptaszyńska; Przemyslaw Latoch; Paul Hurd; Andrew Polaszek; Joanna Michalska-Madej; Łukasz Grochowalski; Dominik Strapagiel; Sebastian Gnat; Daniel Załuski; Marek Gancarz; Robert Rusinek; Patcharin Krutmuang; Raquel Martín Hernández; Mariano Higes Pascual; Agata Starosta. Amplicon Sequencing of Variable 16S rRNA from Bacteria and ITS2 Regions from Fungi and Plants, Reveals Honeybee Susceptibility to Diseases Results from Their Forage Availability under Anthropogenic Landscapes. Pathogens 2021, 10, 381 .
AMA StyleAneta Ptaszyńska, Przemyslaw Latoch, Paul Hurd, Andrew Polaszek, Joanna Michalska-Madej, Łukasz Grochowalski, Dominik Strapagiel, Sebastian Gnat, Daniel Załuski, Marek Gancarz, Robert Rusinek, Patcharin Krutmuang, Raquel Martín Hernández, Mariano Higes Pascual, Agata Starosta. Amplicon Sequencing of Variable 16S rRNA from Bacteria and ITS2 Regions from Fungi and Plants, Reveals Honeybee Susceptibility to Diseases Results from Their Forage Availability under Anthropogenic Landscapes. Pathogens. 2021; 10 (3):381.
Chicago/Turabian StyleAneta Ptaszyńska; Przemyslaw Latoch; Paul Hurd; Andrew Polaszek; Joanna Michalska-Madej; Łukasz Grochowalski; Dominik Strapagiel; Sebastian Gnat; Daniel Załuski; Marek Gancarz; Robert Rusinek; Patcharin Krutmuang; Raquel Martín Hernández; Mariano Higes Pascual; Agata Starosta. 2021. "Amplicon Sequencing of Variable 16S rRNA from Bacteria and ITS2 Regions from Fungi and Plants, Reveals Honeybee Susceptibility to Diseases Results from Their Forage Availability under Anthropogenic Landscapes." Pathogens 10, no. 3: 381.
The petroleum ether crude extracts of A. conyzoides (Pe-Ac) were used to treat three medically intimidating pests of Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus, to evaluate their non-target screening against the mosquito predator. The chemical scanning of Pe-Ac through GC-MS analysis revealed a total of nine compounds and the maximum peak area was observed in 1,5-Heptadien-3-yne (22.14%). At the maximum dosage of Pe-Ac (200 ppm), significant larvicidal activity was shown against the fourth instars of Ae. aegypti (96%), An. stephensi (93%), and Cx. quinquefasciatus (92%) respectively. The percentages of oviposition deterrence index (ODI) of all three mosquito vectors are maximum at the highest sub-lethal dosage of Pe-Ac (75 ppm) and minimum at the control dosage. The sub-lethal dosage blocked the activity of carboxylesterase activity and upregulated the detoxifying enzyme activity in a dose-dependent way. The adulticidal activity of Pe-Ac showed that the maximum adult mortality rate (100%) was recorded at the prominent dosage of Pe-Ac 600 ppm against the vectors of all three mosquitos at the maximum adulticidal time of 30 min. Histopathological investigation of fourth instar larvae of all three mosquitos treated with a sub-lethal dosage of Pe-Ac showed that the midgut cells (epithelium, lumen, and peritrophic matrix) are ruptured completely whereas they appear to be normal in control larvae. The non-toxicity evaluation of Pe-Ac compared with the chemical toxin Temephos in aquatic predator Toxorhynchites splendens revealed that the plant extracts are harmless even at the prominent dosage (1000 ppm) as compared to Temephos (1 and 2 ppm) and displayed a higher mortality rate against the mosquito predators. Thus the safety index recommends that the Pe-Ac is more explicit to targets and a suitable auxiliary to chemical pesticides.
Venkatachalam Ramasamy; Sengodan Karthi; Raja Ganesan; Palanisamy Prakash; Sengottayan Senthil-Nathan; Subramaniam Umavathi; Patcharin Krutmuang; Prabhakaran Vasantha-Srinivasan. Chemical characterization of billy goat weed extracts Ageratum conyzoides (Asteraceae) and their mosquitocidal activity against three blood-sucking pests and their non-toxicity against aquatic predators. Environmental Science and Pollution Research 2021, 28, 28456 -28469.
AMA StyleVenkatachalam Ramasamy, Sengodan Karthi, Raja Ganesan, Palanisamy Prakash, Sengottayan Senthil-Nathan, Subramaniam Umavathi, Patcharin Krutmuang, Prabhakaran Vasantha-Srinivasan. Chemical characterization of billy goat weed extracts Ageratum conyzoides (Asteraceae) and their mosquitocidal activity against three blood-sucking pests and their non-toxicity against aquatic predators. Environmental Science and Pollution Research. 2021; 28 (22):28456-28469.
Chicago/Turabian StyleVenkatachalam Ramasamy; Sengodan Karthi; Raja Ganesan; Palanisamy Prakash; Sengottayan Senthil-Nathan; Subramaniam Umavathi; Patcharin Krutmuang; Prabhakaran Vasantha-Srinivasan. 2021. "Chemical characterization of billy goat weed extracts Ageratum conyzoides (Asteraceae) and their mosquitocidal activity against three blood-sucking pests and their non-toxicity against aquatic predators." Environmental Science and Pollution Research 28, no. 22: 28456-28469.
European Apis mellifera and Asian Apis cerana honeybees, are essential crop pollinators. Microbiome studies can provide complex information on health and fitness of these insects in relation to environmental changes, and plant availability. Amplicon sequencing of variable regions of 16S rRNA and internally transcribed spacers (ITSs) allow identification of the metabiome. These methods provide a tool for monitoring otherwise uncultured microbes isolated from the gut of the honeybees. They also help monitor the composition of the gut fungi and, intriguingly, pollens collected by the insect. Here, we present data from amplicon sequencing of the 16S rRNA and ITS2 regions from honeybees collected at various time points from anthropogenic landforms as urban areas in Poland, UK, Spain, Greece, and Thailand. We have analysed microbial composition of honeybee intestine as well as fungi and pollens. We conclude that differences between samples were mainly influenced by the bacteria, plant pollens and fungi, respectively. Moreover, honeybees feeding on a honeydew diet, mainly based on sugars, were more prone to fungal pathogens (Nosema ceranae) and neogregarines. Finally, the period when honeybees switch to the winter generation (longer-lived forager honeybees) is the most sensitive to diet perturbations and hence pathogens attack, for the whole beekeeping season. It is possible that evolutionary adaptation of bees fails to benefit them in the modern anthropomorphised environment.
Aneta A. Ptaszyńska; Przemysław Latoch; Paul J. Hurd; Andrew Polaszek; Joanna Michalska-Madej; Łukasz Grochowalski; Dominik Strapagiel; Sebastian Gnat; Daniel Załuski; Marek Gancarz; Robert Rusinek; Patcharin Krutmuang; Raquel Martín Hernández; Mariano Higes Pascual; Agata L. Starosta. Amplicon sequencing of variable 16S rRNA and ITS2 regions reveal honeybee susceptibility to diseases resulted of their dietary preferences under anthropogenic landforms. 2021, 1 .
AMA StyleAneta A. Ptaszyńska, Przemysław Latoch, Paul J. Hurd, Andrew Polaszek, Joanna Michalska-Madej, Łukasz Grochowalski, Dominik Strapagiel, Sebastian Gnat, Daniel Załuski, Marek Gancarz, Robert Rusinek, Patcharin Krutmuang, Raquel Martín Hernández, Mariano Higes Pascual, Agata L. Starosta. Amplicon sequencing of variable 16S rRNA and ITS2 regions reveal honeybee susceptibility to diseases resulted of their dietary preferences under anthropogenic landforms. . 2021; ():1.
Chicago/Turabian StyleAneta A. Ptaszyńska; Przemysław Latoch; Paul J. Hurd; Andrew Polaszek; Joanna Michalska-Madej; Łukasz Grochowalski; Dominik Strapagiel; Sebastian Gnat; Daniel Załuski; Marek Gancarz; Robert Rusinek; Patcharin Krutmuang; Raquel Martín Hernández; Mariano Higes Pascual; Agata L. Starosta. 2021. "Amplicon sequencing of variable 16S rRNA and ITS2 regions reveal honeybee susceptibility to diseases resulted of their dietary preferences under anthropogenic landforms." , no. : 1.
In the present study, the toxicity and physiological disorders of the essential oil isolated from Artemisia annua flowers were assessed against one of the main insect pests of mulberry, Glyphodes pyloalis Walker, announcing one of the safe and effective alternatives to synthetic pesticides. The LC50 (lethal concentration to kill 50% of tested insects) values of the oral and fumigant bioassays of A. annua essential oil were 1.204 % W/V and 3.343 μL/L air, respectively. The A. annua essential oil, rich in camphor, artemisia ketone, β-selinene, pinocarvone, 1,8-cineole, and α-pinene, caused a significant reduction in digestive and detoxifying enzyme activity of G. pyloalis larvae. The contents of protein, glucose, and triglyceride were also reduced in the treated larvae by oral and fumigant treatments. The immune system in treated larvae was weakened after both oral and fumigation applications compared to the control groups. Histological studies on the midgut and ovaries showed that A. annua essential oil caused an obvious change in the distribution of the principal cells of tissues and reduction in yolk spheres in oocytes. Therefore, it is suggested that the essential oil from A. annua flowers, with wide-range bio-effects on G. pyloalis, be used as an available, safe, effective insecticide in the protection of mulberry.
Marziyeh Oftadeh; Jalal Sendi; Asgar Ebadollahi; William Setzer; Patcharin Krutmuang. Mulberry Protection through Flowering-Stage Essential Oil of Artemisia annua against the Lesser Mulberry Pyralid, Glyphodes pyloalis Walker. Foods 2021, 10, 210 .
AMA StyleMarziyeh Oftadeh, Jalal Sendi, Asgar Ebadollahi, William Setzer, Patcharin Krutmuang. Mulberry Protection through Flowering-Stage Essential Oil of Artemisia annua against the Lesser Mulberry Pyralid, Glyphodes pyloalis Walker. Foods. 2021; 10 (2):210.
Chicago/Turabian StyleMarziyeh Oftadeh; Jalal Sendi; Asgar Ebadollahi; William Setzer; Patcharin Krutmuang. 2021. "Mulberry Protection through Flowering-Stage Essential Oil of Artemisia annua against the Lesser Mulberry Pyralid, Glyphodes pyloalis Walker." Foods 10, no. 2: 210.
Comparative baseline toxicity of chemical pesticides Temephos, along with the crude extracts of Swietenia mahagoni Jacq. (Meliaceae) (Mx-Sm) against the Laboratory strains (LS) and Wild Strains (WS) of Aedes aegypti Linn. and their non-target screening against the aquatic predator was analyzed. The larval mortality was significant in Temephos (0.5 ppm-93.5%) and Mx-Sm (300 ppm-92.10%) and statistically altered with control. Despite this, the larval mortality is relatively higher in LS than WS treated with Temephos in all the treated instars. Both strains LS and WS displayed stable mortality rate across all the instars while treated with Mx-Sm (300 ppm). The sub-lethal concentration of Mx-Sm (205 ppm) showed no significant differences in the level of α- and β-carboxylesterase. But, there is a significant difference observed between both strains treated with Temephos (0.35 ppm). Similar trends were observed in the GST and CYP450 levels, as the enzyme ratio gets uplifted prominently in the WS compared to the LS in Temephos treatment. Whereas the enzyme regulation up-lifted steadily in both LS and WS treated with Mx-Sm. The non-target screening of Mx-Sm and Temephos against the mosquito predator Toxorhynchites splendens revealed that the Mx-Sm was less toxic at the prominent dosage 1000 ppm as compared to Temephos (1 ppm). Thus the safety index recommends that Mx-Sm was a target-specific and suitable alternative for chemical pesticides.
Prabhakaran Vasantha-Srinivasan; Sengodan Karthi; Raja Ganesan; Sengottayan Senthil-Nathan; Patcharin Krutmuang; Muthiah Chellappandian; Narayanaswamy Radhakrishnan; Athirstam Ponsankar; Kalvikkarasan Karthick; Abdul-Raheem Nelofer. The efficacy of methanolic extract of Swietenia mahagoni Jacq. (Meliaceae) and a commercial insecticide against laboratory and field strains of Aedes aegypti (Linn.) and their impact on its predator Toxorhnchites splendens. Biocatalysis and Agricultural Biotechnology 2021, 31, 101915 .
AMA StylePrabhakaran Vasantha-Srinivasan, Sengodan Karthi, Raja Ganesan, Sengottayan Senthil-Nathan, Patcharin Krutmuang, Muthiah Chellappandian, Narayanaswamy Radhakrishnan, Athirstam Ponsankar, Kalvikkarasan Karthick, Abdul-Raheem Nelofer. The efficacy of methanolic extract of Swietenia mahagoni Jacq. (Meliaceae) and a commercial insecticide against laboratory and field strains of Aedes aegypti (Linn.) and their impact on its predator Toxorhnchites splendens. Biocatalysis and Agricultural Biotechnology. 2021; 31 ():101915.
Chicago/Turabian StylePrabhakaran Vasantha-Srinivasan; Sengodan Karthi; Raja Ganesan; Sengottayan Senthil-Nathan; Patcharin Krutmuang; Muthiah Chellappandian; Narayanaswamy Radhakrishnan; Athirstam Ponsankar; Kalvikkarasan Karthick; Abdul-Raheem Nelofer. 2021. "The efficacy of methanolic extract of Swietenia mahagoni Jacq. (Meliaceae) and a commercial insecticide against laboratory and field strains of Aedes aegypti (Linn.) and their impact on its predator Toxorhnchites splendens." Biocatalysis and Agricultural Biotechnology 31, no. : 101915.
Fish meal (FM) is no longer a sustainable source for the increasing aquaculture industry. Animal proteins from insects may be used as a FM alternative source as long as they do not create adverse effects in fish. Black soldier fly larvae meal (BSFLM) was tested in a 12-week experiment on Nile tilapia (Oreochromis niloticus). Four hundred and twenty (14.77 ± 2.09 g) fish were divided into seven groups and were fed seven diets: control (0% BSFLM-100% FM), and FM replaced by BSFLM at rates of 10%, 20%, 40%, 60%, 80% and 100%. Growth indexes, feed utilization efficiency indices, feed intake, and survival rate were not significantly different (p > 0.05) between FM and BSFLM fed fish. Values of red blood cell, white blood cells, hemoglobin, hematocrit, mean corpuscular volume and hemoglobin, mean corpuscular hemoglobin concentration, red blood cell distribution width, and platelet values were not affected by BSFLM. Skin, mucus lysozyme, and peroxidase activities were improved in BSFLM fed fish. BSFLM can be used as a substitution for FM in the Nile tilapia (O. niloticus) diet at up to a 100% rate with no adverse effects.
Nisarat Tippayadara; Mahmoud Dawood; Patcharin Krutmuang; Seyed Hoseinifar; Hien Doan; Marina Paolucci. Replacement of Fish Meal by Black Soldier Fly (Hermetia illucens) Larvae Meal: Effects on Growth, Haematology, and Skin Mucus Immunity of Nile Tilapia, Oreochromis niloticus. Animals 2021, 11, 193 .
AMA StyleNisarat Tippayadara, Mahmoud Dawood, Patcharin Krutmuang, Seyed Hoseinifar, Hien Doan, Marina Paolucci. Replacement of Fish Meal by Black Soldier Fly (Hermetia illucens) Larvae Meal: Effects on Growth, Haematology, and Skin Mucus Immunity of Nile Tilapia, Oreochromis niloticus. Animals. 2021; 11 (1):193.
Chicago/Turabian StyleNisarat Tippayadara; Mahmoud Dawood; Patcharin Krutmuang; Seyed Hoseinifar; Hien Doan; Marina Paolucci. 2021. "Replacement of Fish Meal by Black Soldier Fly (Hermetia illucens) Larvae Meal: Effects on Growth, Haematology, and Skin Mucus Immunity of Nile Tilapia, Oreochromis niloticus." Animals 11, no. 1: 193.
Due to the detrimental side-effects of synthetic pesticides, the use of nonchemical strategies in the management of insect pests is necessary. In the present study, the susceptibility of fourteen eggplant cultivars to green peach aphid (M. persicae) were investigated. According to preliminary screening tests, ‘Long-Green’, ‘Ravaya’ and ‘Red-Round’ as relatively resistant, and ‘White-Casper’ and ‘Pearl-Round’ as susceptible cultivars were recognized. In the antixenosis tests, the highest hosting preference was documented for ‘White-Casper’. Population growth parameters were used for evaluation of antibiosis. The highest and lowest developmental time (d) was observed on ‘Long-Green’ (4.33 d) and ‘White-Casper’ (3.26 d), respectively. The highest and lowest intrinsic rates of population increase (rm) were on ‘White-Casper’ (0.384 d−1) and ‘Long-Green’ (0.265 d−1), respectively. Significant differences were observed in the height and fresh and dry weight of infested and noninfected plants. Plant resistance index (PRI), as a simplified way to assess all resistance mechanisms, provides a particular value to determine the proper resistant cultivar. The greatest PRI value was observed on ‘Long-Green’. In general, the ‘Long-Green’ showed the least, and the ‘White-Casper’ displayed the most susceptibility among tested cultivars infested by M. persicae, which might be useful in integrated management of this pest.
Zienab Raeyat; Jabraiel Razmjou; Bahram Naseri; Asgar Ebadollahi; Patcharin Krutmuang. Evaluation of the Susceptibility of Some Eggplant Cultivars to Green Peach Aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae). Agriculture 2021, 11, 31 .
AMA StyleZienab Raeyat, Jabraiel Razmjou, Bahram Naseri, Asgar Ebadollahi, Patcharin Krutmuang. Evaluation of the Susceptibility of Some Eggplant Cultivars to Green Peach Aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae). Agriculture. 2021; 11 (1):31.
Chicago/Turabian StyleZienab Raeyat; Jabraiel Razmjou; Bahram Naseri; Asgar Ebadollahi; Patcharin Krutmuang. 2021. "Evaluation of the Susceptibility of Some Eggplant Cultivars to Green Peach Aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae)." Agriculture 11, no. 1: 31.
The present investigation aimed to determine the fungal toxicity of Isaria tenuipes (My-It) against the dengue mosquito vector Aedes aegypti L. and its non-target impact against the aquatic predator Toxorhynchitessplendens. Lethal concentrations (LC50 and LC90) of My-It were observed in 2.27 and 2.93 log ppm dosages, respectively. The sub-lethal dosage (My-It-1 × 104 conidia/mL) displayed a significant oviposition deterrence index and also blocked the fecundity rate of dengue mosquitos in a dose-dependent manner. The level of major detoxifying enzymes, such as carboxylesterase (α-and β-) and SOD, significantly declined in both third and fourth instar larvae at the maximum dosage of My-It 1 × 105 conidia/mL. However, the level of glutathione S-transferase (GST) and cytochrome P-450 (CYP450) declined steadily when the sub-lethal dosage was increased and attained maximum reduction in the enzyme level at the dosage of My-It (1 × 105 conidia/mL). Correspondingly, the gut-histology and photomicrography results made evident that My-It (1 × 105 conidia/mL) heavily damaged the internal gut cells and external physiology of the dengue larvae compared to the control. Moreover, the non-target toxicity against the beneficial predator revealed that My-It at the maximum dosage (1 × 1020 conidia/mL) was found to be less toxic with Tx.splendens. Thus, the present toxicological research on Isaria tenuipes showed that it is target-specific and a potential agent for managing medically threatening arthropods.
Sengodan Karthi; Prabhakaran Vasantha-Srinivasan; Raja Ganesan; Venkatachalam Ramasamy; Sengottayan Senthil-Nathan; Hanem F. Khater; Narayanaswamy Radhakrishnan; Kesavan Amala; Tae-Jin Kim; Mohamed A. El-Sheikh; Patcharin Krutmuang. Target Activity of Isaria tenuipes (Hypocreales: Clavicipitaceae) Fungal Strains against Dengue Vector Aedes aegypti (Linn.) and Its Non-Target Activity Against Aquatic Predators. Journal of Fungi 2020, 6, 196 .
AMA StyleSengodan Karthi, Prabhakaran Vasantha-Srinivasan, Raja Ganesan, Venkatachalam Ramasamy, Sengottayan Senthil-Nathan, Hanem F. Khater, Narayanaswamy Radhakrishnan, Kesavan Amala, Tae-Jin Kim, Mohamed A. El-Sheikh, Patcharin Krutmuang. Target Activity of Isaria tenuipes (Hypocreales: Clavicipitaceae) Fungal Strains against Dengue Vector Aedes aegypti (Linn.) and Its Non-Target Activity Against Aquatic Predators. Journal of Fungi. 2020; 6 (4):196.
Chicago/Turabian StyleSengodan Karthi; Prabhakaran Vasantha-Srinivasan; Raja Ganesan; Venkatachalam Ramasamy; Sengottayan Senthil-Nathan; Hanem F. Khater; Narayanaswamy Radhakrishnan; Kesavan Amala; Tae-Jin Kim; Mohamed A. El-Sheikh; Patcharin Krutmuang. 2020. "Target Activity of Isaria tenuipes (Hypocreales: Clavicipitaceae) Fungal Strains against Dengue Vector Aedes aegypti (Linn.) and Its Non-Target Activity Against Aquatic Predators." Journal of Fungi 6, no. 4: 196.
The larvicidal potential of crude leaf extracts of Rhizophora mucronata, the red mangrove, using diverse solvent extracts of the plant against the early fourth instar larvae of Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti mosquito vectors was analyzed. The acetone extract of R. mucronata showed the greatest efficacy: for Cx. quinquefasciatus (LC50 = 0.13 mg/mL; LC90 = 2.84 mg/mL), An. stephensi (LC50 = 0.34 mg/mL; LC90 = 6.03 mg/mL), and Ae. aegypti (LC50 = 0.11 mg/mL; LC90 = 1.35 mg/mL). The acetone extract was further fractionated into four fractions and tested for its larvicidal activity. Fraction 3 showed stronger larvicidal activity against all the three mosquito larvae. Chemical characterization of the acetone extract displayed the existence of several identifiable compounds like phytol, 3,7,11,15-tetramethyl-2-hexadecen-1-ol, 1-hexyl-2-nitrocyclohexane, eicosanoic acid etc. Enzyme assay displayed that R. mucronata active F3-fractions exert divergent effects on all three mosquitos’ biochemical defensive mechanisms. The plant fractions displayed significant repellent activity against all the three mosquito vectors up to the maximum repellent time of 210 min. Thus, the bioactive molecules in the acetone extract of R. murconata leaves showed significant larvicidal and enzyme inhibitory activity and displayed novel eco-friendly tool for mosquito control.
Sengodan Karthi; Karthic Uthirarajan; Vinothkumar Manohar; Manigandan Venkatesan; Kamaraj Chinnaperumal; Prabhakaran Vasantha-Srinivasan; Patcharin Krutmuang. Larvicidal Enzyme Inhibition and Repellent Activity of Red Mangrove Rhizophora mucronata (Lam.) Leaf Extracts and Their Biomolecules Against Three Medically Challenging Arthropod Vectors. Molecules 2020, 25, 3844 .
AMA StyleSengodan Karthi, Karthic Uthirarajan, Vinothkumar Manohar, Manigandan Venkatesan, Kamaraj Chinnaperumal, Prabhakaran Vasantha-Srinivasan, Patcharin Krutmuang. Larvicidal Enzyme Inhibition and Repellent Activity of Red Mangrove Rhizophora mucronata (Lam.) Leaf Extracts and Their Biomolecules Against Three Medically Challenging Arthropod Vectors. Molecules. 2020; 25 (17):3844.
Chicago/Turabian StyleSengodan Karthi; Karthic Uthirarajan; Vinothkumar Manohar; Manigandan Venkatesan; Kamaraj Chinnaperumal; Prabhakaran Vasantha-Srinivasan; Patcharin Krutmuang. 2020. "Larvicidal Enzyme Inhibition and Repellent Activity of Red Mangrove Rhizophora mucronata (Lam.) Leaf Extracts and Their Biomolecules Against Three Medically Challenging Arthropod Vectors." Molecules 25, no. 17: 3844.
Spodoptera litura are important agricultural pest, and they have a worldwide distribution. The microbiota gut is vital for the host with significant activity in metabolism. Lepidopteran species is one of the prominent orders in insect group of phytophagous pests, and their linkage with microbes are presently practiced and others with future perspectives. In this present investigation of gut microbiota isolation and identification from larvae of S. litura and to assess the midgut bacteria role of in promoting survival to pesticides. Based on the preliminary screening, we screened three different bacterial species isolated from the gut microbial communities of S. litura, viz. gram-positive bacteria Clostridium botulinum, Clostridium butyricum, gram-negative bacteria Pseudomonas putida. Present study the detection of bacteria in the midgut by using general microbial technique and the 16s rRNA sequence was used for characterizing the selected microbes. Besides, the bacterial DNA quality was tested by using agarose gel electrophoresis techniques. Phylogenetic analysis using the neighbor-joining tree shows similarity with other bacterial genera. And also pesticide tolerant ability was analyzed with isolated midgut bacterial communities of the bioassay method. It concludes the C. botulinum bacterium was tested for its role in protecting S. litura larvae against cypermethrin toxicity. The results displayed that S. litura survived well in the combinations of cypermethrin along with C. botulinum. Pesticide metabolism in insects, helping to survive in unfavorable environments.
Sengodan Karthi; Babyshalini Panneerselvam; Sengottayan Senthil-Nathan; Prabhakaran Vasantha-Srinivasan; Muthugoundar Subramaniam Shivakumar; Patcharin Krutmuang. Functional identification and characterization of midgut microbial flora derived from lepidopteran larvae Spodoptera litura Fab. Biocatalysis and Agricultural Biotechnology 2020, 28, 101758 .
AMA StyleSengodan Karthi, Babyshalini Panneerselvam, Sengottayan Senthil-Nathan, Prabhakaran Vasantha-Srinivasan, Muthugoundar Subramaniam Shivakumar, Patcharin Krutmuang. Functional identification and characterization of midgut microbial flora derived from lepidopteran larvae Spodoptera litura Fab. Biocatalysis and Agricultural Biotechnology. 2020; 28 ():101758.
Chicago/Turabian StyleSengodan Karthi; Babyshalini Panneerselvam; Sengottayan Senthil-Nathan; Prabhakaran Vasantha-Srinivasan; Muthugoundar Subramaniam Shivakumar; Patcharin Krutmuang. 2020. "Functional identification and characterization of midgut microbial flora derived from lepidopteran larvae Spodoptera litura Fab." Biocatalysis and Agricultural Biotechnology 28, no. : 101758.
Entomopathogenic fungi (EPF) are fungal species that are pathogenic to insects. These fungal pathogens play an important part in controlling insect population making them the first insect pest control factors. Currently, more than 750 species of fungi from around 90 genera are known to be pathogenic to insects classified in several phyla namely: Chytridiomycota, Ascomycota, Basidiomycota, and the subphylum Entomophthoromycotina. The EPFs contain a plethora of advantages such as being environmentally safe, can be mass-produced, and having the ability to infect the insect hosts through the cuticle instead of waiting for ingestion in order to cause infection. Moreover, it has been established that they can target almost every stage of insect cycle, making them a unique component in the integrated pest management approaches. This review is aimed at finding out how these microorganisms have been studied and adopted for biological control in Southeast Asia and Africa and their possible utilization in integrated pest management. From the published literature, it is apparent that EPFs are ubiquitous both in Southeast Asia and Africa. These fungi have been isolated in various habitats and identified, but unfortunately, there are not many formulations produced that can be commercially utilized in agriculture and forestry. Therefore, concerted efforts are needed so that we can adopt the usage of these important fungi in controlling insect pests and also implement them in Integrated Pest Management (IPM).
Julius Rajula; Afroja Rahman; Patcharin Krutmuang. Entomopathogenic fungi in Southeast Asia and Africa and their possible adoption in biological control. Biological Control 2020, 151, 104399 .
AMA StyleJulius Rajula, Afroja Rahman, Patcharin Krutmuang. Entomopathogenic fungi in Southeast Asia and Africa and their possible adoption in biological control. Biological Control. 2020; 151 ():104399.
Chicago/Turabian StyleJulius Rajula; Afroja Rahman; Patcharin Krutmuang. 2020. "Entomopathogenic fungi in Southeast Asia and Africa and their possible adoption in biological control." Biological Control 151, no. : 104399.
The crude n-hexane extracts Epaltes pygmaea (NH-Epx) displayed a steady larval toxicity against Culex quinquefasciatus and Aedes aegypti at the maximum dosage of 125 ppm. The level of α- and β carboxylesterase activity was downregulated to the sub-lethal dosage of NH-EPx (75 ppm) in both vectors. The level of GST and CYP450 gets upregulated. The LC50 and the LC90 of NH-EPx were observed at 63 and 117 ppm and 73 and 120 ppm dosage against both the vectors, respectively. NH-EPx treatment showed morphological alterations in the gut tissues. The non-target screening recommends that NH-EPx is safe against aquatic predator.
Kesavan Amala; Raja Ganesan; Seengodan Karthi; Sengottayan Senthil-Nathan; Muthiah Chellappandian; Patcharin Krutmunag; Narayanaswamy Radhakrishnan; Faruq Mohammad; Athirstam Ponsankar; Prabhakaran Vasantha-Srinivasan. Larval and gut enzyme toxicity of n-hexane extract Epaltes pygmaea DC. against the arthropod vectors and its non-toxicity against aquatic predator. Toxin Reviews 2020, 1 -11.
AMA StyleKesavan Amala, Raja Ganesan, Seengodan Karthi, Sengottayan Senthil-Nathan, Muthiah Chellappandian, Patcharin Krutmunag, Narayanaswamy Radhakrishnan, Faruq Mohammad, Athirstam Ponsankar, Prabhakaran Vasantha-Srinivasan. Larval and gut enzyme toxicity of n-hexane extract Epaltes pygmaea DC. against the arthropod vectors and its non-toxicity against aquatic predator. Toxin Reviews. 2020; ():1-11.
Chicago/Turabian StyleKesavan Amala; Raja Ganesan; Seengodan Karthi; Sengottayan Senthil-Nathan; Muthiah Chellappandian; Patcharin Krutmunag; Narayanaswamy Radhakrishnan; Faruq Mohammad; Athirstam Ponsankar; Prabhakaran Vasantha-Srinivasan. 2020. "Larval and gut enzyme toxicity of n-hexane extract Epaltes pygmaea DC. against the arthropod vectors and its non-toxicity against aquatic predator." Toxin Reviews , no. : 1-11.
Larval toxicity of ethanolic extract of C. parvula (Ex-Cp) was prominent in the second and the third instars at the maximum lethal dosage of 100 ppm with 98 and 97 % mortality rate respectively. The LC50 and LC90 was displayed at 43 ppm and 88 ppm dosage respectively. Correspondingly, the sub-lethal dosage (65 ppm) of Ex-Cp significantly alters the carboxylesterase (α and β), GST and CYP450 enzyme level in both III and IV instar larvae in dose-dependent manner. Similarly, the Ex-Cp displayed significant repellent activity (97 %) with a maximum level of protection time (210 min). Photomicrography assay of Ex-Cp (65 ppm) were toxic to dengue larvae as compared to control. The non-target toxicity of Ex-Cp against the beneficial mosquito predators displayed less toxicity at the maximum dosage of 600 ppm as compared to Temephos. Thus the present research delivers the target and non-target toxicity of red algae C. parvula against the dengue mosquito vector.
Parthiban Yogarajalakshmi; Thinnaur Venugopal Poonguzhali; Raja Ganesan; Sengodan Karthi; Sengottayan Senthil-Nathan; Patcharin Krutmuang; Narayanaswamy Radhakrishnan; Faruq Mohammad; Tae-Jin Kim; Prabhakaran Vasantha-Srinivasan. Toxicological screening of marine red algae Champia parvula (C. Agardh) against the dengue mosquito vector Aedes aegypti (Linn.) and its non-toxicity against three beneficial aquatic predators. Aquatic Toxicology 2020, 222, 105474 .
AMA StyleParthiban Yogarajalakshmi, Thinnaur Venugopal Poonguzhali, Raja Ganesan, Sengodan Karthi, Sengottayan Senthil-Nathan, Patcharin Krutmuang, Narayanaswamy Radhakrishnan, Faruq Mohammad, Tae-Jin Kim, Prabhakaran Vasantha-Srinivasan. Toxicological screening of marine red algae Champia parvula (C. Agardh) against the dengue mosquito vector Aedes aegypti (Linn.) and its non-toxicity against three beneficial aquatic predators. Aquatic Toxicology. 2020; 222 ():105474.
Chicago/Turabian StyleParthiban Yogarajalakshmi; Thinnaur Venugopal Poonguzhali; Raja Ganesan; Sengodan Karthi; Sengottayan Senthil-Nathan; Patcharin Krutmuang; Narayanaswamy Radhakrishnan; Faruq Mohammad; Tae-Jin Kim; Prabhakaran Vasantha-Srinivasan. 2020. "Toxicological screening of marine red algae Champia parvula (C. Agardh) against the dengue mosquito vector Aedes aegypti (Linn.) and its non-toxicity against three beneficial aquatic predators." Aquatic Toxicology 222, no. : 105474.
Sengodan Karthi; Manohar Vinothkumar; Uthirarajan Karthic; Venkatesan Manigandan; Ramachandran Saravanan; Prabhakaran Vasantha-Srinivasan; Chinnaperumal Kamaraj; Muthugounder S Shivakumar; Surajit De Mandal; Arumugam Velusamy; Patcharin Krutmuang; Sengottayan Senthil-Nathan. Biological effects of Avicennia marina (Forssk.) vierh. extracts on physiological, biochemical, and antimicrobial activities against three challenging mosquito vectors and microbial pathogens. 2020, 1 .
AMA StyleSengodan Karthi, Manohar Vinothkumar, Uthirarajan Karthic, Venkatesan Manigandan, Ramachandran Saravanan, Prabhakaran Vasantha-Srinivasan, Chinnaperumal Kamaraj, Muthugounder S Shivakumar, Surajit De Mandal, Arumugam Velusamy, Patcharin Krutmuang, Sengottayan Senthil-Nathan. Biological effects of Avicennia marina (Forssk.) vierh. extracts on physiological, biochemical, and antimicrobial activities against three challenging mosquito vectors and microbial pathogens. . 2020; ():1.
Chicago/Turabian StyleSengodan Karthi; Manohar Vinothkumar; Uthirarajan Karthic; Venkatesan Manigandan; Ramachandran Saravanan; Prabhakaran Vasantha-Srinivasan; Chinnaperumal Kamaraj; Muthugounder S Shivakumar; Surajit De Mandal; Arumugam Velusamy; Patcharin Krutmuang; Sengottayan Senthil-Nathan. 2020. "Biological effects of Avicennia marina (Forssk.) vierh. extracts on physiological, biochemical, and antimicrobial activities against three challenging mosquito vectors and microbial pathogens." , no. : 1.
Mosquitoes are principal vector of several vector-borne diseases affecting human beings leading to thousands of deaths per year and responsible for transmitting diseases like malaria, dengue, chikungunya, yellow fever, Zika virus, Japanese encephalitis, and lymphatic filariasis. In the present study, we evaluated the different solvent extracts of mangrove Avicennia marina for their toxicity against larvae of three major mosquito vectors, as well as selected microbial pathogens. The larvicidal mortality of third instars was observed after 24 h. Highest larval mortality was found for the acetone extract of A. marina against Culex quinquefasciatus (LC50 = 0.197 mg/ml; LC90 = 1.5011 mg/ml), Anopheles stephensi (LC50 = 0.176 mg/ml; LC90 = 3.6290 mg/ml), and Aedes aegypti (LC50 = 0.164 mg/ml; LC90 = 4.3554 mg/ml). GC-MS analysis of acetone extract revealed 5 peaks, i.e., 1-hexyl-2-nitrocyclohexane (3.229%), eicosanoic acid (40.582%), cis-9-hexadecenal (70.54%), oleic acid (4.646%), and di-N-decylsulfone (5.136%). Parallel to larvicidal assay, sub-lethal dosage acetone extracts severely affected the enzyme regulations (α,β-carboxylesterase, GST and CYP450) of third instars. Larval and pupal durations increased in all treatment sub-lethal dosage (0.127, 0.151, 0.177, and 0.197 mg/ml), whereas egg hatchability and means of fecundity decreased compared to control. The survival rate was reduced statistically in Cx. quinquefasciatus (χ2 = 23.77, df = 1, P = 0.001) in all the treatment dosages as compared to the control. Antimicrobial activity assays showed significant growth inhibition post treatment with acetone and methanol extracts against Salmonella typhimurium, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus pneumoniae, Escherichia coli, and Shigella flexneri. Overall, these results indicated the potential employment of A. marina extracts as a source of natural mosquitocidal and antimicrobial compounds of green-based environment.
Sengodan Karthi; Manohar Vinothkumar; Uthirarajan Karthic; Venkatesan Manigandan; Ramachandran Saravanan; Prabhakaran Vasantha-Srinivasan; Chinnaperumal Kamaraj; Muthugounder S. Shivakumar; Surajit De Mandal; Arumugam Velusamy; Patcharin Krutmuang; Sengottayan Senthil-Nathan. Biological effects of Avicennia marina (Forssk.) vierh. extracts on physiological, biochemical, and antimicrobial activities against three challenging mosquito vectors and microbial pathogens. Environmental Science and Pollution Research 2020, 27, 15174 -15187.
AMA StyleSengodan Karthi, Manohar Vinothkumar, Uthirarajan Karthic, Venkatesan Manigandan, Ramachandran Saravanan, Prabhakaran Vasantha-Srinivasan, Chinnaperumal Kamaraj, Muthugounder S. Shivakumar, Surajit De Mandal, Arumugam Velusamy, Patcharin Krutmuang, Sengottayan Senthil-Nathan. Biological effects of Avicennia marina (Forssk.) vierh. extracts on physiological, biochemical, and antimicrobial activities against three challenging mosquito vectors and microbial pathogens. Environmental Science and Pollution Research. 2020; 27 (13):15174-15187.
Chicago/Turabian StyleSengodan Karthi; Manohar Vinothkumar; Uthirarajan Karthic; Venkatesan Manigandan; Ramachandran Saravanan; Prabhakaran Vasantha-Srinivasan; Chinnaperumal Kamaraj; Muthugounder S. Shivakumar; Surajit De Mandal; Arumugam Velusamy; Patcharin Krutmuang; Sengottayan Senthil-Nathan. 2020. "Biological effects of Avicennia marina (Forssk.) vierh. extracts on physiological, biochemical, and antimicrobial activities against three challenging mosquito vectors and microbial pathogens." Environmental Science and Pollution Research 27, no. 13: 15174-15187.