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The crucial need for safe and healthy aquatic animals obligates researchers in aquaculture to investigate alternative and beneficial additives. Medicinal herbals and their extracts are compromised with diverse effects on the performances of aquatic animals. These compounds can affect growth performance and stimulate the immune system when used in fish diet. In addition, the use of medicinal herbs and their extracts can reduce oxidative stress induced by several stressors during fish culture. Correspondingly, aquatic animals could gain increased resistance against infectious pathogens and environmental stressors. Nevertheless, the exact mode of action where these additives can affect aquatic animals’ performances is still not well documented. Understanding the mechanistic role of herbal supplements and their derivatives is a vital tool to develop further the strategies and application of these additives for feasible and sustainable aquaculture. Gene-related studies have clarified the detailed information on the herbal supplements’ mode of action when administered orally in aquafeed. Several review articles have presented the potential roles of medicinal herbs on the performances of aquatic animals. However, this review article discusses the outputs of studies conducted on aquatic animals fed dietary, medicinal herbs, focusing on the gene expression related to growth and immune performances. Furthermore, a particular focus is directed to the expected influence of herbal supplements on the reproduction of aquatic animals.
Ehsan Ahmadifar; Hamideh Pourmohammadi Fallah; Morteza Yousefi; Mahmoud Dawood; Seyed Hoseinifar; Hossein Adineh; Sevdan Yilmaz; Marina Paolucci; Hien Doan. The Gene Regulatory Roles of Herbal Extracts on the Growth, Immune System, and Reproduction of Fish. Animals 2021, 11, 2167 .
AMA StyleEhsan Ahmadifar, Hamideh Pourmohammadi Fallah, Morteza Yousefi, Mahmoud Dawood, Seyed Hoseinifar, Hossein Adineh, Sevdan Yilmaz, Marina Paolucci, Hien Doan. The Gene Regulatory Roles of Herbal Extracts on the Growth, Immune System, and Reproduction of Fish. Animals. 2021; 11 (8):2167.
Chicago/Turabian StyleEhsan Ahmadifar; Hamideh Pourmohammadi Fallah; Morteza Yousefi; Mahmoud Dawood; Seyed Hoseinifar; Hossein Adineh; Sevdan Yilmaz; Marina Paolucci; Hien Doan. 2021. "The Gene Regulatory Roles of Herbal Extracts on the Growth, Immune System, and Reproduction of Fish." Animals 11, no. 8: 2167.
Pectins are a group of carbohydrates found in structural parts of terrestrial plants with wide industrial and biomedical applications. This study was designed to investigate the dietary effects of apple pomace-derived pectin (APDP) in rainbow trout (Oncorhynchus mykiss). Four formulated diets were provided with different inclusion levels of APDP for 30 days: 0, 5, 10, and 20 g kg−1; referred to as control, P1, P2, and P3, respectively. In this study, 300 fish (3.56 ± 0.007 g) were randomly distributed into twelve fiberglass tanks and fed 3% of their respective body weight four times a day. At the end of the experiment, growth parameters, including weight gain, specific growth rate, and food conversion ratio (FCR) were significantly improved in P1 and P2 treatments compared to those of the other treatments. Results from proximate composition analysis showed that protein content increased, and lipid decreased in the P2 and P3 groups. Serum lysozyme, complement activity, total immunoglobulin levels, and total protein were significantly enhanced in all treatments compared to those of the control group. Gene expression results showed no significant difference in regulation of interleukin-1β (IL-1β); however, up-regulation of lysozyme, interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α) was observed in both P1 and P2. Unlike lipase, the activity of protease and amylase significantly increased in fish receiving different levels of APDP compared to the control (p< 0.05). In conclusion, the present findings suggest APDA as a promising feed additive for rainbow trout.
Seyed Hoseinifar; Ghasem Rashidian; Hamed Ghafarifarsani; Mohammad Jahazi; Mehdi Soltani; Hien Doan; Ehab El-Haroun; Marina Paolucci. Effects of Apple (Malus pomila) Pomace-Derived Pectin on the Innate Immune Responses, Expressions of Key Immune-Related Genes, Growth Performance, and Digestive Enzyme Activity of Rainbow Trout (Oncorhynchus mykiss). Animals 2021, 11, 2117 .
AMA StyleSeyed Hoseinifar, Ghasem Rashidian, Hamed Ghafarifarsani, Mohammad Jahazi, Mehdi Soltani, Hien Doan, Ehab El-Haroun, Marina Paolucci. Effects of Apple (Malus pomila) Pomace-Derived Pectin on the Innate Immune Responses, Expressions of Key Immune-Related Genes, Growth Performance, and Digestive Enzyme Activity of Rainbow Trout (Oncorhynchus mykiss). Animals. 2021; 11 (7):2117.
Chicago/Turabian StyleSeyed Hoseinifar; Ghasem Rashidian; Hamed Ghafarifarsani; Mohammad Jahazi; Mehdi Soltani; Hien Doan; Ehab El-Haroun; Marina Paolucci. 2021. "Effects of Apple (Malus pomila) Pomace-Derived Pectin on the Innate Immune Responses, Expressions of Key Immune-Related Genes, Growth Performance, and Digestive Enzyme Activity of Rainbow Trout (Oncorhynchus mykiss)." Animals 11, no. 7: 2117.
Grape harvesting generates a high amount of wastes, mostly leaves, which represent an economic and ecological problem for farmers. New products can be generated through these wastes, giving environmental, social, and economic advantages while also meeting the industry demand for novel natural ingredients. In this study, aqueous leaf-extracts from two cultivars of Vitis vinifera Aglianico (Agl) and Greco di Tufo (Gre) were produced by microwave-assisted extraction (MAE) and evaluated in composition by ATR- FTIR and HPLC to identify the main phenolic compounds, especially quercetin and kaempferol. The results showed that leaves extracts confirm to be a potential source of phenolic compounds. Dry extracts, although highly functional, show critical handling characteristics, being sticky and unstable in normal post-processing conditions. A stable and easy handling microparticulate ingredient was produced by spray drying containing the most phenolic-rich obtained extract (AGL-28). The microparticle powder form based on pectin/maltodextrin matrix was produced with high process efficiency. The microstructures were able to confer functional and chemical stability to the extract while also showing good technological characteristics (high water dissolution rate and flow properties), transforming the extract into a handling ingredient able to meet new industrial uses.
Tiziana Esposito; Marina Paolucci; Francesca Sansone; Teresa Mencherini; Severina Pacifico; Maria Volpe. Exploitation and Valorization of Agro-Food Wastes from Grape Harvesting: Production, Characterization of MAE-Extracts from Vitis vinifera Leaves and Stabilization in Microparticulate Powder Form. Applied Sciences 2021, 11, 5827 .
AMA StyleTiziana Esposito, Marina Paolucci, Francesca Sansone, Teresa Mencherini, Severina Pacifico, Maria Volpe. Exploitation and Valorization of Agro-Food Wastes from Grape Harvesting: Production, Characterization of MAE-Extracts from Vitis vinifera Leaves and Stabilization in Microparticulate Powder Form. Applied Sciences. 2021; 11 (13):5827.
Chicago/Turabian StyleTiziana Esposito; Marina Paolucci; Francesca Sansone; Teresa Mencherini; Severina Pacifico; Maria Volpe. 2021. "Exploitation and Valorization of Agro-Food Wastes from Grape Harvesting: Production, Characterization of MAE-Extracts from Vitis vinifera Leaves and Stabilization in Microparticulate Powder Form." Applied Sciences 11, no. 13: 5827.
The aim of the present study was to test the possible ameliorative efficacy of phytochemicals such as tannins on intestinal inflammation and dysbiosis. The effect of a chestnut shell (Castanea sativa) extract (CSE) rich in polyphenols, mainly represented by tannins, on k-carrageenan-induced intestinal inflammation in adult zebrafish (Danio rerio) was tested in a feeding trial. Intestinal inflammation was induced by 0.1% k-carrageenan added to the diet for 10 days. CSE was administered for 10 days after k-carrageenan induced inflammation. The intestinal morphology and histopathology, cytokine expression, and microbiota were analyzed. The k-carrageenan treatment led to gut lumen expansion, reduction of intestinal folds, and increase of the goblet cells number, accompanied by the upregulation of pro-inflammatory factors (TNFα, COX2) and alteration in the number and ratio of taxonomic groups of bacteria. CSE counteracted the inflammatory status enhancing the growth of health helpful bacteria (Enterobacteriaceae and Pseudomonas), decreasing the pro-inflammatory factors, and activating the anti-inflammatory cytokine IL-10. In conclusion, CSE acted as a prebiotic on zebrafish gut microbiota, sustaining the use of tannins as food additives to ameliorate the intestinal inflammation. Our results may be relevant for both aquaculture and medical clinic fields.
Graziella Orso; Mikhail Solovyev; Serena Facchiano; Evgeniia Tyrikova; Daniela Sateriale; Elena Kashinskaya; Caterina Pagliarulo; Hossein Hoseinifar; Evgeniy Simonov; Ettore Varricchio; Marina Paolucci; Roberta Imperatore. Chestnut Shell Tannins: Effects on Intestinal Inflammation and Dysbiosis in Zebrafish. Animals 2021, 11, 1538 .
AMA StyleGraziella Orso, Mikhail Solovyev, Serena Facchiano, Evgeniia Tyrikova, Daniela Sateriale, Elena Kashinskaya, Caterina Pagliarulo, Hossein Hoseinifar, Evgeniy Simonov, Ettore Varricchio, Marina Paolucci, Roberta Imperatore. Chestnut Shell Tannins: Effects on Intestinal Inflammation and Dysbiosis in Zebrafish. Animals. 2021; 11 (6):1538.
Chicago/Turabian StyleGraziella Orso; Mikhail Solovyev; Serena Facchiano; Evgeniia Tyrikova; Daniela Sateriale; Elena Kashinskaya; Caterina Pagliarulo; Hossein Hoseinifar; Evgeniy Simonov; Ettore Varricchio; Marina Paolucci; Roberta Imperatore. 2021. "Chestnut Shell Tannins: Effects on Intestinal Inflammation and Dysbiosis in Zebrafish." Animals 11, no. 6: 1538.
This study investigated the effects of dietary arginine supplementation on plasma ammonia and urea levels, and immune‐ and antioxidant‐related gene expressions of common carp (Cyprinus carpio), exposed to ambient ammonia. Fish (10.5 ± 0.74 g) were fed diets containing arginine (0: control diet, 0.25: 0.25Arg and 0.5%: 0.5Arg) for 14 days and then subjected to ammonia exposure for three hours. The results showed that arginine significantly decreased plasma ammonia level, whereas increased the plasma urea level. Arginine supplementation significantly up‐regulated head kidney il1b, il10, tnfa and liver sod, cat, gpx and gst gene expressions, whereas significantly down‐regulated hsp70 gene expression in liver. Ammonia exposure led to a significant increase in plasma ammonia and urea levels. There were elevations in head kidney il1b, and liver sod, cat, gpx, gst and hsp70 gene expression in fish after challenged with ammonia. The interaction effects of arginine supplementation and ammonia exposure on head kidney il10, and liver gst and hsp70 gene expressions were observed, as arginine prevented ammonia‐induced down‐regulation in il10 expression, mitigated ammonia‐induced up‐regulation in hsp70 expression and intensified up‐regulation in gst expression. In conclusion, it is suggested that two‐week supplementation of arginine (0.5% of diet) is useful to mitigate the adverse effects of ambient ammonia when in the farm, common carp is at risk of ammonia toxicity.
Morteza Yousefi; Behrooz Abtahi; Hossein Adineh; Seyed Hossein Hoseinifar; Ali Taheri Mirghaed; Marina Paolucci; Hien Van Doan. Effects of dietary arginine supplementation on cytokine‐ and antioxidant‐related gene expressions in common carp ( Cyprinus carpio ) fingerling during ammonia toxicity. Aquaculture Research 2021, 52, 2751 -2758.
AMA StyleMorteza Yousefi, Behrooz Abtahi, Hossein Adineh, Seyed Hossein Hoseinifar, Ali Taheri Mirghaed, Marina Paolucci, Hien Van Doan. Effects of dietary arginine supplementation on cytokine‐ and antioxidant‐related gene expressions in common carp ( Cyprinus carpio ) fingerling during ammonia toxicity. Aquaculture Research. 2021; 52 (6):2751-2758.
Chicago/Turabian StyleMorteza Yousefi; Behrooz Abtahi; Hossein Adineh; Seyed Hossein Hoseinifar; Ali Taheri Mirghaed; Marina Paolucci; Hien Van Doan. 2021. "Effects of dietary arginine supplementation on cytokine‐ and antioxidant‐related gene expressions in common carp ( Cyprinus carpio ) fingerling during ammonia toxicity." Aquaculture Research 52, no. 6: 2751-2758.
This Special Issue of Foods, The Effect of Novel Packaging Technology on Food Safety and Quality, contains seven papers that were refereed and selected in accordance with the usual editorial standards of the journal
Marina Paolucci; Maria Volpe. The Effect of Novel Packaging Technology on Food Safety and Quality. Foods 2021, 10, 269 .
AMA StyleMarina Paolucci, Maria Volpe. The Effect of Novel Packaging Technology on Food Safety and Quality. Foods. 2021; 10 (2):269.
Chicago/Turabian StyleMarina Paolucci; Maria Volpe. 2021. "The Effect of Novel Packaging Technology on Food Safety and Quality." Foods 10, no. 2: 269.
Fish meal (FM) is no longer a sustainable source for the increasing aquaculture industry. Animal proteins from insects may be used as a FM alternative source as long as they do not create adverse effects in fish. Black soldier fly larvae meal (BSFLM) was tested in a 12-week experiment on Nile tilapia (Oreochromis niloticus). Four hundred and twenty (14.77 ± 2.09 g) fish were divided into seven groups and were fed seven diets: control (0% BSFLM-100% FM), and FM replaced by BSFLM at rates of 10%, 20%, 40%, 60%, 80% and 100%. Growth indexes, feed utilization efficiency indices, feed intake, and survival rate were not significantly different (p > 0.05) between FM and BSFLM fed fish. Values of red blood cell, white blood cells, hemoglobin, hematocrit, mean corpuscular volume and hemoglobin, mean corpuscular hemoglobin concentration, red blood cell distribution width, and platelet values were not affected by BSFLM. Skin, mucus lysozyme, and peroxidase activities were improved in BSFLM fed fish. BSFLM can be used as a substitution for FM in the Nile tilapia (O. niloticus) diet at up to a 100% rate with no adverse effects.
Nisarat Tippayadara; Mahmoud Dawood; Patcharin Krutmuang; Seyed Hoseinifar; Hien Doan; Marina Paolucci. Replacement of Fish Meal by Black Soldier Fly (Hermetia illucens) Larvae Meal: Effects on Growth, Haematology, and Skin Mucus Immunity of Nile Tilapia, Oreochromis niloticus. Animals 2021, 11, 193 .
AMA StyleNisarat Tippayadara, Mahmoud Dawood, Patcharin Krutmuang, Seyed Hoseinifar, Hien Doan, Marina Paolucci. Replacement of Fish Meal by Black Soldier Fly (Hermetia illucens) Larvae Meal: Effects on Growth, Haematology, and Skin Mucus Immunity of Nile Tilapia, Oreochromis niloticus. Animals. 2021; 11 (1):193.
Chicago/Turabian StyleNisarat Tippayadara; Mahmoud Dawood; Patcharin Krutmuang; Seyed Hoseinifar; Hien Doan; Marina Paolucci. 2021. "Replacement of Fish Meal by Black Soldier Fly (Hermetia illucens) Larvae Meal: Effects on Growth, Haematology, and Skin Mucus Immunity of Nile Tilapia, Oreochromis niloticus." Animals 11, no. 1: 193.
Fresh figs are very sensitive to microbial spoilage, even in cold storage conditions. Thus, fresh figs are high perishable products during postharvest with microbiological decay that induces an unpleasant taste and smell due to rot, and suitable conservation methods must therefore be applied. The fruit usually is consumed fresh locally, dried, or preserved longer term in other transformed forms. A sustainable approach to extend the shelf-life of figs can be constituted by application of an edible coating able to maintain the quality of the fruit during storage. A comparison between fresh figs in a commercial preservation system, with the figs preserved in an edible coating, and an active edible coating to preserve their quality characteristics was carried out. The coating efficacy was enhanced with the addition of pomegranate peel extract at two different concentrations. The inclusion of a component with high antioxidant activity in an edible coating proved to be an excellent method for preserving the quality of this highly perishable fruit. The application of natural products, obtained from renewable sources, represents a simple and economic strategy, but also a tool capable of preserving the quality of the fruit during the postharvest storage, which is often consumed in production areas due to shelf-life problems.
Marina Paolucci; Michele Di Stasio; Alida Sorrentino; Francesco La Cara; Maria Grazia Volpe. Active Edible Polysaccharide-Based Coating for Preservation of Fresh Figs (Ficus carica L.). Foods 2020, 9, 1793 .
AMA StyleMarina Paolucci, Michele Di Stasio, Alida Sorrentino, Francesco La Cara, Maria Grazia Volpe. Active Edible Polysaccharide-Based Coating for Preservation of Fresh Figs (Ficus carica L.). Foods. 2020; 9 (12):1793.
Chicago/Turabian StyleMarina Paolucci; Michele Di Stasio; Alida Sorrentino; Francesco La Cara; Maria Grazia Volpe. 2020. "Active Edible Polysaccharide-Based Coating for Preservation of Fresh Figs (Ficus carica L.)." Foods 9, no. 12: 1793.
A growing body of evidence suggests that the biological effects of polyphenols are not restricted to antioxidant activity, but they exert a wide range of modulatory effects on metabolic pathways, cellular signaling and gene expression. In this study, we tested the minimum safe concentration of gallic acid (GA) in 72 hpf zebrafish larvae in order to evaluate the effects on the central nervous system and the behavioral response. We showed that a short exposure (30 min) induces the depletion of the two main excitatory and inhibitory neurotransmitters, Glu and GABA, respectively, in the larval nervous system. The acute impairment of GABAergic‐glutamatergic balance was paralleled by an increase of the fosab neuronal activity marker in specific brain areas, such as the forebrain, olfactory bulbs, pallial area, ventral midbrain, tegmentum, and the medulla oblongata ventral area. The neuronal excitation was mirrored by the increased cumulative motor response. The inhibition of the olfactory epithelium with brief cadmium exposition suggests a direct involvement of olfaction in the larvae response to GA. Our results demonstrate that a brief exposure to GA induces motoneuronal hyperexcitability in zebrafish. The behavioral response was probably elicited through the activation of an odorous, or chemical, stimulus. The specificity of the activated neuronal territories suggests the involvement of additional signaling pathways. Although the underlying molecular mechanisms remain to be elucidated, our data support the hypothesis that GA acts as an excitatory molecule, capable of inducing a specific nerve response. These results offer a new vision on potential effects of GA.
Giovanni Annona; Andrea Tarallo; Valeria Nittoli; Ettore Varricchio; Paolo Sordino; Salvatore D'Aniello; Marina Paolucci. Short‐term exposure to the simple polyphenolic compound gallic acid induces neuronal hyperactivity in zebrafish larvae. European Journal of Neuroscience 2020, 53, 1367 -1377.
AMA StyleGiovanni Annona, Andrea Tarallo, Valeria Nittoli, Ettore Varricchio, Paolo Sordino, Salvatore D'Aniello, Marina Paolucci. Short‐term exposure to the simple polyphenolic compound gallic acid induces neuronal hyperactivity in zebrafish larvae. European Journal of Neuroscience. 2020; 53 (5):1367-1377.
Chicago/Turabian StyleGiovanni Annona; Andrea Tarallo; Valeria Nittoli; Ettore Varricchio; Paolo Sordino; Salvatore D'Aniello; Marina Paolucci. 2020. "Short‐term exposure to the simple polyphenolic compound gallic acid induces neuronal hyperactivity in zebrafish larvae." European Journal of Neuroscience 53, no. 5: 1367-1377.
A 63‐day experiment was done to study the effects of four levels (5, 10, 20 and 50 g/kg) of encapsulated organic salts (Na‐acetate, Na‐butyrate, Na‐lactate and Na‐propionate) on the growth indices and haemato‐immunological responses of crayfish Astacus leptodactylus leptodactylus (4.38 ± 0.08 g). Crayfish were distributed at 51 1,000‐L tanks (17 treatments at triplicate). The highest values of final weight (27.86 g), specific growth rate (2.94% body weight per day) and survival rate (96%) were observed in the crayfish fed the 20 g/kg of encapsulated Na‐propionate diet (p < .05). The highest activities of phenoloxidase (7.4 U/min), superoxide dismutase (7.80 U/min) and lysozyme (9.40 U/min) were observed in the gut of crayfish fed the 20 g/kg of encapsulated Na‐propionate diet (p < .05), as well as the highest activities of alkaline protease (10.70 U/mg), lipase (9.10 U/mg), amylase (9.60 U/mg) and the lactobacillus count (p < .05). Broken line regression model of SGR and phenoloxidase activity suggested that the optimum dietary levels of encapsulated Na‐acetate, Na‐butyrate, Na‐lactate and Na‐propionate could be 30.7, 31.8, 31.4 and 33.5 g/kg, respectively, in crayfish reared in culture conditions.
Omid Safari; Marina Paolucci; Hamidreza Ahmadniaye Motlagh. Effect of dietary encapsulated organic salts (Na‐acetate, Na‐butyrate, Na‐lactate and Na‐propionate) on growth performance, haemolymph, antioxidant and digestive enzyme activities and gut microbiota of juvenile narrow clawed crayfish, Astacus leptodactylus leptodactylus Eschscholtz, 1823. Aquaculture Nutrition 2020, 27, 91 -104.
AMA StyleOmid Safari, Marina Paolucci, Hamidreza Ahmadniaye Motlagh. Effect of dietary encapsulated organic salts (Na‐acetate, Na‐butyrate, Na‐lactate and Na‐propionate) on growth performance, haemolymph, antioxidant and digestive enzyme activities and gut microbiota of juvenile narrow clawed crayfish, Astacus leptodactylus leptodactylus Eschscholtz, 1823. Aquaculture Nutrition. 2020; 27 (1):91-104.
Chicago/Turabian StyleOmid Safari; Marina Paolucci; Hamidreza Ahmadniaye Motlagh. 2020. "Effect of dietary encapsulated organic salts (Na‐acetate, Na‐butyrate, Na‐lactate and Na‐propionate) on growth performance, haemolymph, antioxidant and digestive enzyme activities and gut microbiota of juvenile narrow clawed crayfish, Astacus leptodactylus leptodactylus Eschscholtz, 1823." Aquaculture Nutrition 27, no. 1: 91-104.
The 3,5-diiodo-L-thyronine (3,5-T2) is an endogenous metabolite of thyroid hormones, whose administration to rodents fed high-fat diet (HFD) prevents body weight increase and reverts the expression pattern of pro-inflammatory factors associated to HFD. The diet-induced obese (D.I.O.) zebrafish (Danio rerio) has been recently used as an experimental model to investigate fundamental processes underlying central and peripheral obesity-driven inflammation. Herein, we aim to understand the role of 3,5-T2 in regulating central and peripheral inflammation in D.I.O. model of zebrafish. 3,5-T2 (10 nM and 100 nM) was administered with the obesity-inducing diet (D.I.O. with 3,5-T2) or after 4 weeks of obesity-inducing diet (D.I.O. flw 3,5-T2). 3,5-T2 significantly increased the body weight and serum triglyceride levels in D.I.O. zebrafish in both conditions. Moreover, 3,5-T2 sustained or increased inflammation in the anterior (AI) and mid (MI) intestine when administered with the obesity-inducing diet, as indicated by the immunoexpression of the inflammatory markers tumor-necrosis factor-α (TNFα), cyclooxygenase 2 (COX2), calnexin, caspase 3, and proliferating cell nuclear antigen (PCNA). On the contrary, when 3,5-T2 was administered after the obesity-inducing diet, partly reverted the intestinal alteration induced by D.I.O. In addition, brain inflammation, as indicated by the increase in the activation of microglia, was detected in D.I.O. zebrafish and D.I.O. treated with 3,5-T2. These findings reveal that the effects of 3,5-T2 on fish intestine and brain can deviate from those shown in obese mammals, opening new avenues to the investigation of the potential impact of this thyroid metabolite in different diseases including obesity.
Roberta Imperatore; Lea Tunisi; Isabella Mavaro; Livia D’Angelo; Chiara Attanasio; Omid Safari; Hamidreza Ahmadniaye Motlagh; Paolo De Girolamo; Luigia Cristino; Ettore Varricchio; Marina Paolucci. Immunohistochemical Analysis of Intestinal and Central Nervous System Morphology in an Obese Animal Model (Danio rerio) Treated with 3,5-T2: A Possible Farm Management Practice? Animals 2020, 10, 1131 .
AMA StyleRoberta Imperatore, Lea Tunisi, Isabella Mavaro, Livia D’Angelo, Chiara Attanasio, Omid Safari, Hamidreza Ahmadniaye Motlagh, Paolo De Girolamo, Luigia Cristino, Ettore Varricchio, Marina Paolucci. Immunohistochemical Analysis of Intestinal and Central Nervous System Morphology in an Obese Animal Model (Danio rerio) Treated with 3,5-T2: A Possible Farm Management Practice? Animals. 2020; 10 (7):1131.
Chicago/Turabian StyleRoberta Imperatore; Lea Tunisi; Isabella Mavaro; Livia D’Angelo; Chiara Attanasio; Omid Safari; Hamidreza Ahmadniaye Motlagh; Paolo De Girolamo; Luigia Cristino; Ettore Varricchio; Marina Paolucci. 2020. "Immunohistochemical Analysis of Intestinal and Central Nervous System Morphology in an Obese Animal Model (Danio rerio) Treated with 3,5-T2: A Possible Farm Management Practice?" Animals 10, no. 7: 1131.
Freshwater crayfish are bioindicators of environmental pollution, often used for the assessment of heavy metal (HM) presence in the tissues, a time-consuming and expensive task. In this study, we propose the use of the vibrational spectroscopy to detect in a fast, non-destructive and sensitive way the presence of HM in the cephalothorax exoskeleton of the freshwater crayfish. Incorporation of HM into the cephalothorax exoskeleton was investigated under controlled laboratory conditions. In particular, the cephalothorax exoskeleton of five crayfish species (Astacus leptodactylus, Procambarus clarkii, Austropotamobius pallipes, Faxonius limosus, and Pacifastacus leniusculus) was analyzed by attenuated total reflection-Fourier transformed infrared (ATR-FTIR) spectroscopy in the presence or absence of cadmium (Cd), chromium (Cr), lead (Pb), nickel (Ni), and zinc (Zn) up to 4 weeks at various concentrations (0.01, 0.1, 1, 10, ppm). The ATR-FTIR profile of the crayfish cephalothorax exoskeleton was compatible with the presence of amorphous calcium carbonate, chitin, and proteins. The incubation with the HM revealed two main modifications: the shift of the peak from 859 to 872 cm-1 and the appearance of a peak at 712 cm-1. Both are ascribable to the HM interaction with calcium carbonate. The absorbance of both peaks increased along with the time of incubation, and the HM concentration. We conclude that ATR-FTIR analysis can be a useful, quick, and cost-sensitive tool to detect HM presence in the crayfish cephalothorax exoskeleton. However, it has to be regarded as a non-specific analytical technique for assessing HM contamination, since it is unable to discriminate between different HM.
Maria Grazia Volpe; Daniela Ghia; Omid Safari; Marina Paolucci. Fast non-destructive assessment of heavy metal presence by ATR–FTIR analysis of crayfish exoskeleton. Environmental Science and Pollution Research 2020, 27, 21021 -21031.
AMA StyleMaria Grazia Volpe, Daniela Ghia, Omid Safari, Marina Paolucci. Fast non-destructive assessment of heavy metal presence by ATR–FTIR analysis of crayfish exoskeleton. Environmental Science and Pollution Research. 2020; 27 (17):21021-21031.
Chicago/Turabian StyleMaria Grazia Volpe; Daniela Ghia; Omid Safari; Marina Paolucci. 2020. "Fast non-destructive assessment of heavy metal presence by ATR–FTIR analysis of crayfish exoskeleton." Environmental Science and Pollution Research 27, no. 17: 21021-21031.
Algae have multiple similarities with fungi, with both belonging to the Thallophyte, a polyphyletic group of non-mobile organisms grouped together on the basis of similar characteristics, but not sharing a common ancestor. The main difference between algae and fungi is noted in their metabolism. In fact, although algae have chlorophyll-bearing thalloids and are autotrophic organisms, fungi lack chlorophyll and are heterotrophic, not able to synthesize their own nutrients. However, our studies have shown that the extremophilic microalga Galderia sulphuraria (GS) can also grow very well in heterotrophic conditions like fungi. This study was carried out using several approaches such as scanning electron microscope (SEM), gas chromatography/mass spectrometry (GC/MS), and infrared spectrophotometry (ATR-FTIR). Results showed that the GS, strain ACUF 064, cultured in autotrophic (AGS) and heterotrophic (HGS) conditions, produced different biomolecules. In particular, when grown in HGS, the algae (i) was 30% larger, with an increase in carbon mass that was 20% greater than AGS; (ii) produced higher quantities of stearic acid, oleic acid, monounsaturated fatty acids (MUFAs), and ergosterol; (iii) produced lower quantities of fatty acid methyl esters (FAMEs) such as methyl palmytate, and methyl linoleate, saturated fatty acids (SFAs), and poyliunsaturated fatty acids (PUFAs). ATR-FTIR and principal component analysis (PCA) statistical analysis confirmed that the macromolecular content of HGS was significantly different from AGS. The ability to produce different macromolecules by changing the trophic conditions may represent an interesting strategy to induce microalgae to produce different biomolecules that can find applications in several fields such as food, feed, nutraceutical, or energy production.
Roberto Barone; Lorenzo De Napoli; Luciano Mayol; Marina Paolucci; Maria Grazia Volpe; Luigi D’Elia; Antonino Pollio; Marco Guida; Edvige Gambino; Federica Carraturo; Roberta Marra; Francesco Vinale; Sheridan Lois Woo; Matteo Lorito. Autotrophic and Heterotrophic Growth Conditions Modify Biomolecole Production in the Microalga Galdieria sulphuraria (Cyanidiophyceae, Rhodophyta). Marine Drugs 2020, 18, 169 .
AMA StyleRoberto Barone, Lorenzo De Napoli, Luciano Mayol, Marina Paolucci, Maria Grazia Volpe, Luigi D’Elia, Antonino Pollio, Marco Guida, Edvige Gambino, Federica Carraturo, Roberta Marra, Francesco Vinale, Sheridan Lois Woo, Matteo Lorito. Autotrophic and Heterotrophic Growth Conditions Modify Biomolecole Production in the Microalga Galdieria sulphuraria (Cyanidiophyceae, Rhodophyta). Marine Drugs. 2020; 18 (3):169.
Chicago/Turabian StyleRoberto Barone; Lorenzo De Napoli; Luciano Mayol; Marina Paolucci; Maria Grazia Volpe; Luigi D’Elia; Antonino Pollio; Marco Guida; Edvige Gambino; Federica Carraturo; Roberta Marra; Francesco Vinale; Sheridan Lois Woo; Matteo Lorito. 2020. "Autotrophic and Heterotrophic Growth Conditions Modify Biomolecole Production in the Microalga Galdieria sulphuraria (Cyanidiophyceae, Rhodophyta)." Marine Drugs 18, no. 3: 169.
The existence of nutritional and energy reserves is fundamental for fish female fertility, so that the existence of a correlation between metabolic reserves and reproductive capacity is suggested. Leptin regulates body weight and energy homeostasis. Estradiol induces the synthesis of vitellogenin, a phospholipoglycoprotein produced by the liver and taken up by the growing oocytes. The objective of this study was to investigate the possible existence of a crosstalk between 17β-estradiol (E2) and leptin in the modulation of E2-induced vtg in the rainbow trout Oncorhynchus mykiss. Liver slices were incubated with recombinant trout leptin (rt-lep) at three different concentrations (1-10-100 ng/ml). rt-lep brought about the decrease of E2-induced vtg secretion in the medium and the down-regulation of vtg mRNA expression. Moreover, rt-lep stimulated the lipase activity and diminished the liver fatty acid content. The combined employment of signal transduction inhibitors and the analysis of signal transduction phosphorylated factors revealed that rt-lep effect on E2-induced vtg occurred through the activation of phosphodiesterase, protein kinase C, MAP kinases, and protein kinase A. In conclusion, our study suggests that leptin influences E2-induced vtg synthesis in the rainbow trout Oncorhynchus mykiss by modifying both the protein and the lipid components.
Marina Paolucci; Elena Coccia; Roberta Imperatore; Ettore Varricchio. A cross-talk between leptin and 17β-estradiol in vitellogenin synthesis in rainbow trout Oncorhynchus mykiss liver. Fish Physiology and Biochemistry 2019, 46, 331 -344.
AMA StyleMarina Paolucci, Elena Coccia, Roberta Imperatore, Ettore Varricchio. A cross-talk between leptin and 17β-estradiol in vitellogenin synthesis in rainbow trout Oncorhynchus mykiss liver. Fish Physiology and Biochemistry. 2019; 46 (1):331-344.
Chicago/Turabian StyleMarina Paolucci; Elena Coccia; Roberta Imperatore; Ettore Varricchio. 2019. "A cross-talk between leptin and 17β-estradiol in vitellogenin synthesis in rainbow trout Oncorhynchus mykiss liver." Fish Physiology and Biochemistry 46, no. 1: 331-344.
The present study was designed to investigate the effects of dietary apple cider vinegar (ACV) on digestive enzyme activity and growth performance as well as immune responses and antibacterial activity of skin mucus in green terror (Andinoacara rivulatus). Fish were fed diets supplemented with 0%, 1%, 2% and 4% of ACV (40.830 ppm acetic acid concentration) for 63 days. The final weight and weight gain values were observed to be significantly higher in fish fed with 2% of ACV compared to the control group (p < .05). ACV inclusion in the diets had significant effects on SGR (%) and FCR values (p > .05). ACV treatment resulted in a significant increase in the intestinal protease, α‐amylase, lipase and alkaline phosphatase activities compared to control (p < .05). The activities of digestive enzymes in fish fed with 2% and 4% of ACV diets were significantly higher than the other groups (p < .05). The total protein content, alternative haemolytic complement, alkaline phosphatase, total immunoglobulins and lysozyme activities of skin mucus increased significantly in fish fed with ACV diets (p < .05). In conclusion, administration of ACV enhanced digestive enzyme activity, growth performance, immune responses and the immune properties of skin mucus, and it can be used as a natural growth promoter and immunostimulant in green terror culture.
Hamidreza Ahmadniaye Motlagh; Mehrdad Sarkheil; Omid Safari; Marina Paolucci. Supplementation of dietary apple cider vinegar as an organic acidifier on the growth performance, digestive enzymes and mucosal immunity of green terror (Andinoacara rivulatus). Aquaculture Research 2019, 51, 197 -205.
AMA StyleHamidreza Ahmadniaye Motlagh, Mehrdad Sarkheil, Omid Safari, Marina Paolucci. Supplementation of dietary apple cider vinegar as an organic acidifier on the growth performance, digestive enzymes and mucosal immunity of green terror (Andinoacara rivulatus). Aquaculture Research. 2019; 51 (1):197-205.
Chicago/Turabian StyleHamidreza Ahmadniaye Motlagh; Mehrdad Sarkheil; Omid Safari; Marina Paolucci. 2019. "Supplementation of dietary apple cider vinegar as an organic acidifier on the growth performance, digestive enzymes and mucosal immunity of green terror (Andinoacara rivulatus)." Aquaculture Research 51, no. 1: 197-205.
Leaves of Vitis vinifera cv. Greco di Tufo, a precious waste made in the Campania Region (Italy), after vintage harvest, underwent reduction, lyophilization, and ultrasound-assisted maceration in ethanol. The alcoholic extract, as evidenced by a preliminary UHPLC-HR-MS analysis, showed a high metabolic complexity. Thus, the extract was fractionated, obtaining, among others, a fraction enriched in flavonol glycosides and glycuronides. Myricetin, quercetin, kaempferol, and isorhamnetin derivatives were tentatively identified based on their relative retention time and TOF-MS2 data. As the localization of saccharidic moiety in glycuronide compounds proved to be difficult due to the lack of well-established fragmentation pattern and/or the absence of characteristic key fragments, to obtain useful MS information and to eliminate matrix effect redundancies, the isolation of the most abundant extract’s compound was achieved. HR-MS/MS spectra of the compound, quercetin-3-O-glucuronide, allowed us to thoroughly rationalize its fragmentation pattern, and to unravel the main differences between MS/MS behavior of flavonol glycosides and glycuronides. Furthermore, cytotoxicity assessment on the (poly)phenol rich fraction and the pure isolated compound was carried out using central nervous system cell lines. The chemoprotective effect of both the (poly)phenol fraction and quercetin-3-O-glucuronide was evaluated.
Simona Piccolella; Giuseppina Crescente; Maria Grazia Volpe; Marina Paolucci; Severina Pacifico. UHPLC-HR-MS/MS-Guided Recovery of Bioactive Flavonol Compounds from Greco di Tufo Vine Leaves. Molecules 2019, 24, 3630 .
AMA StyleSimona Piccolella, Giuseppina Crescente, Maria Grazia Volpe, Marina Paolucci, Severina Pacifico. UHPLC-HR-MS/MS-Guided Recovery of Bioactive Flavonol Compounds from Greco di Tufo Vine Leaves. Molecules. 2019; 24 (19):3630.
Chicago/Turabian StyleSimona Piccolella; Giuseppina Crescente; Maria Grazia Volpe; Marina Paolucci; Severina Pacifico. 2019. "UHPLC-HR-MS/MS-Guided Recovery of Bioactive Flavonol Compounds from Greco di Tufo Vine Leaves." Molecules 24, no. 19: 3630.
Neurotrophins contribute to the complexity of vertebrate nervous system, being involved in cognition and memory. Abnormalities associated with neurotrophin synthesis may lead to neuropathies, neurodegenerative disorders and age-associated cognitive decline. The genome of teleost fishes contains homologs of some mammalian neurotrophins as well as a gene coding for an additional neurotrophin (NT-6). In this study, we characterized this specific neurotrophin in the short-lived fish Nothobranchius furzeri, a relatively new model for aging studies. Thus, we report herein for the first time the age-related expression of a neurotrophin in a non-mammalian vertebrate. Interestingly, we found comparable expression levels of NT-6 in the brain of both young and old animals. More in detail, we used a locked nucleic acid probe and a riboprobe to investigate the neuroanatomical distribution of NT-6 mRNA revealing a significant expression of the neurotrophin in neurons of the forebrain (olfactory bulbs, dorsal and ventral telencephalon, and several diencephalic nuclei), midbrain (optic tectum, longitudinal tori, and semicircular tori), and hindbrain (valvula and body of cerebellum, reticular formation and octavolateral area of medulla oblongata). By combining in situ hybridization and immunohistochemistry, we showed that NT-6 mRNA is synthesized in mature neurons. These results contribute to better understanding the evolutionary history of neurotrophins in vertebrates, and their role in the adult brain.
Adele Leggieri; Chiara Attanasio; Antonio Palladino; Alessandro Cellerino; Carla Lucini; Marina Paolucci; Eva Terzibasi Tozzini; Paolo De Girolamo; Livia D’Angelo; Terzibasi Tozzini; De Girolamo; D’ Angelo. Identification and Expression of Neurotrophin-6 in the Brain of Nothobranchius furzeri: One More Piece in Neurotrophin Research. Journal of Clinical Medicine 2019, 8, 595 .
AMA StyleAdele Leggieri, Chiara Attanasio, Antonio Palladino, Alessandro Cellerino, Carla Lucini, Marina Paolucci, Eva Terzibasi Tozzini, Paolo De Girolamo, Livia D’Angelo, Terzibasi Tozzini, De Girolamo, D’ Angelo. Identification and Expression of Neurotrophin-6 in the Brain of Nothobranchius furzeri: One More Piece in Neurotrophin Research. Journal of Clinical Medicine. 2019; 8 (5):595.
Chicago/Turabian StyleAdele Leggieri; Chiara Attanasio; Antonio Palladino; Alessandro Cellerino; Carla Lucini; Marina Paolucci; Eva Terzibasi Tozzini; Paolo De Girolamo; Livia D’Angelo; Terzibasi Tozzini; De Girolamo; D’ Angelo. 2019. "Identification and Expression of Neurotrophin-6 in the Brain of Nothobranchius furzeri: One More Piece in Neurotrophin Research." Journal of Clinical Medicine 8, no. 5: 595.
In this study different methods were used to evaluate the effectiveness of a carrageenan coating and carrageenan coating incorporating lemon essential oil (ELO) in preserving the physicochemical and olfactory characteristics of trout fillets stored at 4 °C up to 12 days. The fillet morphological structure was analyzed by histological and immunological methods; lipid peroxidation was performed with the peroxide and thiobarbituric acid reactive substances (TBARS) tests. At the same time, two less time-consuming methods, such as Attenuated Total Reflectance-Fourier Transformed Infrared (ATR-FTIR) spectroscopy and the electronic nose, were used. Uncoated trout fillets (UTF) showed a less compact tissue structure than carrageenan-coated threads (CTF) and coated fillets of carrageenan (active) ELO (ACTF), probably due to the degradation of collagen, as indicated by optical microscopy and ATR-FTIR. UTF showed greater lipid oxidation compared to CTF and ACTF, as indicated by the peroxide and TBARS tests and ATR-FTIR spectroscopy. The carrageenan coating containing ELO preserved the olfactory characteristics of the trout fillets better than the carrageenan coating alone, as indicated by the electronic nose analysis. This study confirms that both carrageenan and ELO containing carrageenan coatings slow down the decay of the physicochemical and olfactory characteristics of fresh trout fillets stored at 4 °C, although the latter is more effective.
Maria Grazia Volpe; Elena Coccia; Francesco Siano; Michele Di Stasio; Marina Paolucci. Rapid Evaluation Methods for Quality of Trout (Oncorhynchus mykiss) Fresh Fillet Preserved in an Active Edible Coating. Foods 2019, 8, 113 .
AMA StyleMaria Grazia Volpe, Elena Coccia, Francesco Siano, Michele Di Stasio, Marina Paolucci. Rapid Evaluation Methods for Quality of Trout (Oncorhynchus mykiss) Fresh Fillet Preserved in an Active Edible Coating. Foods. 2019; 8 (4):113.
Chicago/Turabian StyleMaria Grazia Volpe; Elena Coccia; Francesco Siano; Michele Di Stasio; Marina Paolucci. 2019. "Rapid Evaluation Methods for Quality of Trout (Oncorhynchus mykiss) Fresh Fillet Preserved in an Active Edible Coating." Foods 8, no. 4: 113.
In this study, we utilized a model of trout intestinal explants to investigate the uptake and the metabolic effects of Hydroxytyrosol (HT), a bioactive molecule belonging to polyphenols, present in the fruit and leaf of the olive (Olea europaea L.). Fragments of the pyloric caeca (PC), mid intestine (MI) and distal intestine (DI) of the rainbow trout Oncorhynchus mykiss, were incubated with varying concentrations (2.0–20–200 μM) of HT for 5, 15, 60, 120 and 360 min. HT was uptaken by PC and MI, but not DI. The HT concentration reached the highest value after 15 min of incubation. 2.0 and 20 μM HT were well tolerated by both PC and MI, 200 μM HT was cytotoxic, causing DNA damage in PC, but not in MI. 1H NMR analysis of PC and MI incubated with 2.0 and 20 μM HT revealed the increase in the essential amino acids and fatty acids, suggesting that HT slowed down the amino acid degradation while acting as lipolysis stimulators. The effect of HT on the intestinal macromolecular asset was also investigated by Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) in order to test the advantages of this method in the analysis of biological samples. HT caused macromolecular modifications coherent with the results of the HT uptake, cytotoxicity and 1H NMR analysis. In conclusions, the intestinal explants allow many experimental conditions to be tested simultaneously. Such ex vivo approach may be usefully employed to study the effects of additives or feed components in screening tests before designing in vivo trials. Thus, it may represent an alternative to reduce the number of animals employed. Finally, the present study confirms the validity of ATR-FTIR as a rapid, non-invasive, high-resolution analytical method of analysis for biological samples.
Elena Coccia; Roberta Imperatore; Graziella Orso; Dominique Melck; Ettore Varricchio; Maria Grazia Volpe; Marina Paolucci. Explants of Oncorhynchus mykiss intestine to detect bioactive molecules uptake and metabolic effects: Applications in aquaculture. Aquaculture 2019, 506, 193 -204.
AMA StyleElena Coccia, Roberta Imperatore, Graziella Orso, Dominique Melck, Ettore Varricchio, Maria Grazia Volpe, Marina Paolucci. Explants of Oncorhynchus mykiss intestine to detect bioactive molecules uptake and metabolic effects: Applications in aquaculture. Aquaculture. 2019; 506 ():193-204.
Chicago/Turabian StyleElena Coccia; Roberta Imperatore; Graziella Orso; Dominique Melck; Ettore Varricchio; Maria Grazia Volpe; Marina Paolucci. 2019. "Explants of Oncorhynchus mykiss intestine to detect bioactive molecules uptake and metabolic effects: Applications in aquaculture." Aquaculture 506, no. : 193-204.
In this study, chestnut (Castanea sativa) shell was extracted with different solvents, and immunomodulatory activity was investigated in an in vitro model system using blood and intestinal leukocytes of Oncorhynchus mykiss. Gallic acid (GA) was used as a standard. Chestnut shell extract (CSE) and GA readily entered both blood and intestinal leukocytes. Superoxide anion production and phagocytosis were decreased by low doses of CSE and increased with high doses. CSE and GA differently regulated cytokine expression in blood and intestinal leukocytes. High doses of CSE upregulated IL-1β, TNF-α, and IL-10 in intestinal leukocytes and IL-10 in blood leukocytes. Low doses of CSE upregulated IL-1β and TNF-α in blood leukocytes. GA appeared to be effective only in blood leukocytes. The effects of CSE on pro- and anti-inflammatory cytokines seemed to suggest an alert effect of the immune defense system against a possible infectious agent. The less evident effect of GA in comparison to CSE could have been attributable to the synergistic and/or additive effects of polyphenols in the latter. The immune-stimulating activity of CSE reported here could be useful for future practical applications in fish health.
Elena Coccia; Francesco Siano; Maria Grazia Volpe; Ettore Varricchio; Orhan Tufan Eroldogan; Marina Paolucci. Chestnut Shell Extract Modulates Immune Parameters in the Rainbow Trout Oncorhynchus mykiss. Fishes 2019, 4, 18 .
AMA StyleElena Coccia, Francesco Siano, Maria Grazia Volpe, Ettore Varricchio, Orhan Tufan Eroldogan, Marina Paolucci. Chestnut Shell Extract Modulates Immune Parameters in the Rainbow Trout Oncorhynchus mykiss. Fishes. 2019; 4 (1):18.
Chicago/Turabian StyleElena Coccia; Francesco Siano; Maria Grazia Volpe; Ettore Varricchio; Orhan Tufan Eroldogan; Marina Paolucci. 2019. "Chestnut Shell Extract Modulates Immune Parameters in the Rainbow Trout Oncorhynchus mykiss." Fishes 4, no. 1: 18.