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Dr. Javier Duque
University of Extremadura (Spain)

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0 Cardiology
0 Internal Medicine
0 Small Animal Imaging
0 Leishmaniosis
0 stem cell

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stem cell
Leishmaniosis

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Short Biography

Degree in veterinary medicine from the University of Extremadura (UEx) in 2000 and PhD from the same university in 2013. I first worked as a veterinary surgeon in a private clinic (2000-2005) and later joined the Internal Medicine Service at the UEx Veterinary Teaching Hospital. I am currently a Associate Professor in the Animal Medicine Department at the Faculty of Veterinary Medicine of Cáceres (UEx). I have furthered my training at the hospitals of the faculties of Bristol, Liverpool (United Kingdom) and North Carolina (United States) and at the Virgen del Rocío (Sevilla) and San Pedro de Alcántara (Cáceres) human medicine hospitals. I am a member of the European Society of Veterinary Cardiology (ESVC). I have published articles in several national and international journals about different topics (Transcranial Doppler, Small Animal Cardiology, Small Animal Internal Medicine, Leishmaniosis, Stem Cells).

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Journal article
Published: 10 July 2021 in Animals
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Mesenchymal stem cells have proven to be a promising alternative to conventional steroids to treat canine inflammatory bowel disease (IBD). However, their administration requires a washout period of immunosuppressive drugs that can lead to an exacerbation of the symptoms. Therefore, the feasibility and effects of the combined application of stem cells and prednisone in IBD-dogs without adequate response to corticosteroids was evaluated for the first time in this study over a long- term follow up. Two groups of dogs with IBD, one without treatment and another with prednisone treatment, received a single infusion of stem cells. The clinical indices, albumin and cobalamin were determined prior to the infusion and after one, three, six and 12 months. In both groups, all parameters significantly improved at each time point. In parallel, the steroid dosage was gradually reduced until it was suppressed in all patients a year after the cell therapy. Therefore, cell therapy can significantly and safely improve the disease condition in dogs with IBD receiving or not receiving prednisone. Furthermore, the steroid dosage can be significantly reduced or cancelled after the stem cell infusion. Their beneficial effects are stable over time and are long lasting.

ACS Style

José Cristóbal; Francisco Duque; Jesús Usón-Casaús; Patricia Ruiz; Esther Nieto; Eva Pérez-Merino. Effects of Allogeneic Mesenchymal Stem Cell Transplantation in Dogs with Inflammatory Bowel Disease Treated with and without Corticosteroids. Animals 2021, 11, 2061 .

AMA Style

José Cristóbal, Francisco Duque, Jesús Usón-Casaús, Patricia Ruiz, Esther Nieto, Eva Pérez-Merino. Effects of Allogeneic Mesenchymal Stem Cell Transplantation in Dogs with Inflammatory Bowel Disease Treated with and without Corticosteroids. Animals. 2021; 11 (7):2061.

Chicago/Turabian Style

José Cristóbal; Francisco Duque; Jesús Usón-Casaús; Patricia Ruiz; Esther Nieto; Eva Pérez-Merino. 2021. "Effects of Allogeneic Mesenchymal Stem Cell Transplantation in Dogs with Inflammatory Bowel Disease Treated with and without Corticosteroids." Animals 11, no. 7: 2061.

Original research
Published: 26 April 2021 in Veterinary Record
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Background The association between myocardial parasitic load (MPL) and cardiac biomarkers in Canine Leishmaniasis (CanL) has not been studied. Methods Dogs with advanced CanL were prospectively recruited and were included if they were euthanised. Prior to euthanasia these variables were assessed: hematocrit, globulin, creatinine, N‐terminal‐pro brain natriuretic peptide (NT‐proBNP), cardiac troponin I (cTnI), blood pressure, urine protein/creatinine ratio and echocardiographic parameters. A left ventricular (LV) sample was taken for histopathology and MPL evaluation by quantitative PCR. Correlation of MPL with all variables was analysed. Dogs with lower and higher histopathology scores were compared. Results Ten dogs were included. NT‐proBNP was 6946 pmol/ (interquartile range [IQR] 3751–9268 pmol/L) and cTnI 4.56 ng/mL (IQR 0.46–13.1 ng/mL). In all dogs, echocardiography showed an increase in LV thickening, and histopathology revealed moderate to severe lympho‐plasmocytic myocarditis and/or myocardial cell degeneration. MPL was 215.53 parasites/gram (IQR 21.2–1372.63 parasites/gram). A strong correlation (p < 0.001; R = 0.90; R20.81) with cTnI was observed but correlation with any of the other variables or differences between the two histopathological scores, were not detected. Conclusions MPL in dogs with advanced CanL shows variable but generally high levels. A strong association between MPL and cTnI was observed, which encourages the exploration of cTnI as a marker in CanL.

ACS Style

Domingo Casamián‐Sorrosal; Rafael Barrera‐Chacón; Sonja Fonfara; Silvia Belinchón‐Lorenzo; Luis Gómez‐Gordo; Javier Galapero‐Arroyo; Javier Fernández‐Cotrina; Jose Ignacio Cristobal‐Verdejo; Francisco Javier Duque. Association of myocardial parasitic load with cardiac biomarkers and other selected variables in 10 dogs with advanced Canine Leishmaniasis. Veterinary Record 2021, e198 .

AMA Style

Domingo Casamián‐Sorrosal, Rafael Barrera‐Chacón, Sonja Fonfara, Silvia Belinchón‐Lorenzo, Luis Gómez‐Gordo, Javier Galapero‐Arroyo, Javier Fernández‐Cotrina, Jose Ignacio Cristobal‐Verdejo, Francisco Javier Duque. Association of myocardial parasitic load with cardiac biomarkers and other selected variables in 10 dogs with advanced Canine Leishmaniasis. Veterinary Record. 2021; ():e198.

Chicago/Turabian Style

Domingo Casamián‐Sorrosal; Rafael Barrera‐Chacón; Sonja Fonfara; Silvia Belinchón‐Lorenzo; Luis Gómez‐Gordo; Javier Galapero‐Arroyo; Javier Fernández‐Cotrina; Jose Ignacio Cristobal‐Verdejo; Francisco Javier Duque. 2021. "Association of myocardial parasitic load with cardiac biomarkers and other selected variables in 10 dogs with advanced Canine Leishmaniasis." Veterinary Record , no. : e198.

Journal article
Published: 19 February 2021 in Animals
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The aim was to evaluate if medetomidine and dexmedetomidine affected arterial ovarian blood flow in dogs. The dogs were randomly assigned to two different groups. In Group 1, medetomidine (10 µg/kg) was administered intramuscularly and, in Group 2, dexmedetomidine (5 µg/kg) was used. After a preliminary exam, arterial blood pressure (BP) was measured and a duplex Doppler ultrasonographic examination of both ovarian arteries was performed. Twenty minutes after the administration of medetomidine or dexmedetomidine, BP and ovarian Doppler ultrasonography were repeated. High quality tracings of ovarian artery flow velocity were obtained in all dogs and Doppler parameters: Peak Systolic Velocity (PSV), End Diastolic Velocity (EDV) and Resistive Index (RI) were measured before and after drug administration in the left (LO) and right (RO) ovaries. PSV and EDV values decreased significantly after drug administration (p < 0.05) compared to the non-sedated values, but no differences were found between the LO and RO (p > 0.05). The RI was not affected by drugs administration in neither of the groups studied (p > 0.05). In conclusion, the administration of medetomidine or dexmedetomidine causes a decrease in blood flow velocity in the ovarian artery and may be a good choice to avoid excessive bleeding prior surgeries in which ovariectomy.

ACS Style

Paloma Nicolás-Barceló; Martina Facchin; Fernando Martínez-Taboada; Rafael Barrera; José Cristóbal; Mario González; Ángela Durán-Galea; Beatriz Macías-García; Francisco Duque. Effects of Sedation with Medetomidine and Dexmedetomidine on Doppler Measurements of Ovarian Artery Blood Flow in Bitches. Animals 2021, 11, 538 .

AMA Style

Paloma Nicolás-Barceló, Martina Facchin, Fernando Martínez-Taboada, Rafael Barrera, José Cristóbal, Mario González, Ángela Durán-Galea, Beatriz Macías-García, Francisco Duque. Effects of Sedation with Medetomidine and Dexmedetomidine on Doppler Measurements of Ovarian Artery Blood Flow in Bitches. Animals. 2021; 11 (2):538.

Chicago/Turabian Style

Paloma Nicolás-Barceló; Martina Facchin; Fernando Martínez-Taboada; Rafael Barrera; José Cristóbal; Mario González; Ángela Durán-Galea; Beatriz Macías-García; Francisco Duque. 2021. "Effects of Sedation with Medetomidine and Dexmedetomidine on Doppler Measurements of Ovarian Artery Blood Flow in Bitches." Animals 11, no. 2: 538.

Original research article
Published: 25 September 2020 in Frontiers in Pharmacology
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More than a century has passed since the first surgical mesh for hernia repair was developed, and, to date, this is still the most widely used method despite the great number of complications it poses. The purpose of this study was to combine stem cell therapy and laparoscopy for the treatment of congenital hernia in a swine animal model. Porcine bone marrow-derived mesenchymal stem cells (MSCs) were seeded on polypropylene surgical meshes using a fibrin sealant solution as a vehicle. Meshes with (cell group) or without (control group) MSCs were implanted through laparoscopy in Large White pigs with congenital abdominal hernia after the approximation of hernia borders (implantation day). A successive laparoscopic biopsy of the mesh and its surrounding tissues was performed a week after implantation, and surgical meshes were excised a month after implantation. Ultrasonography was used to measure hernia sizes. Flow cytometry, histological, and gene expression analyses of the biopsy and necropsy samples were performed. The fibrin sealant solution was easy to prepare and preserved the viability of MSCs in the surgical meshes. Ultrasonography demonstrated a significant reduction in hernia size 1 week after implantation in the cell group relative to that on the day of implantation (p < 0.05). Flow cytometry of the mesh-infiltrated cells showed a non-significant increase of M2 macrophages when the cell group was compared with the control group 1 week after implantation. A significant decrease in the gene expression of VEGF and a significant increase in TNF expression were determined in the cell group 1 month after implantation compared with gene expressions in the control group (p < 0.05). Here, we propose an easy and feasible method to combine stem cell therapy and minimally invasive surgical techniques for hernia repair. In this study, stem cell therapy did not show a great immunomodulatory or regenerative effect in overcoming hernia-related complications. However, our clinically relevant animal model with congenital hernia closely resembles the clinical human condition. Further studies should be focused on this valuable animal model to evaluate stem cell therapies in hernia surgery.

ACS Style

Federica Marinaro; Javier G. Casado; Rebeca Blázquez; Mauricio Veloso Brun; Ricardo Marcos; Marta Santos; Francisco Javier Duque; Esther López; Verónica Álvarez; Alejandra Usón; Francisco Miguel Sánchez-Margallo. Laparoscopy for the Treatment of Congenital Hernia: Use of Surgical Meshes and Mesenchymal Stem Cells in a Clinically Relevant Animal Model. Frontiers in Pharmacology 2020, 11, 1 .

AMA Style

Federica Marinaro, Javier G. Casado, Rebeca Blázquez, Mauricio Veloso Brun, Ricardo Marcos, Marta Santos, Francisco Javier Duque, Esther López, Verónica Álvarez, Alejandra Usón, Francisco Miguel Sánchez-Margallo. Laparoscopy for the Treatment of Congenital Hernia: Use of Surgical Meshes and Mesenchymal Stem Cells in a Clinically Relevant Animal Model. Frontiers in Pharmacology. 2020; 11 ():1.

Chicago/Turabian Style

Federica Marinaro; Javier G. Casado; Rebeca Blázquez; Mauricio Veloso Brun; Ricardo Marcos; Marta Santos; Francisco Javier Duque; Esther López; Verónica Álvarez; Alejandra Usón; Francisco Miguel Sánchez-Margallo. 2020. "Laparoscopy for the Treatment of Congenital Hernia: Use of Surgical Meshes and Mesenchymal Stem Cells in a Clinically Relevant Animal Model." Frontiers in Pharmacology 11, no. : 1.

Journal article
Published: 01 December 2019 in The Canadian veterinary journal = La revue veterinaire canadienne
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ACS Style

Matías C Lucero; Francisco Javier Duque; María Gil; Patricia Ruiz; Beatriz Macias Garcia; José Igancio Cristóbal; Concepción Zaragoza; Rafael Barrera. A plasma calcium-phosphorus product can be used to predict the lifespan of dogs with chronic kidney disease. The Canadian veterinary journal = La revue veterinaire canadienne 2019, 60, 1319 -1325.

AMA Style

Matías C Lucero, Francisco Javier Duque, María Gil, Patricia Ruiz, Beatriz Macias Garcia, José Igancio Cristóbal, Concepción Zaragoza, Rafael Barrera. A plasma calcium-phosphorus product can be used to predict the lifespan of dogs with chronic kidney disease. The Canadian veterinary journal = La revue veterinaire canadienne. 2019; 60 (12):1319-1325.

Chicago/Turabian Style

Matías C Lucero; Francisco Javier Duque; María Gil; Patricia Ruiz; Beatriz Macias Garcia; José Igancio Cristóbal; Concepción Zaragoza; Rafael Barrera. 2019. "A plasma calcium-phosphorus product can be used to predict the lifespan of dogs with chronic kidney disease." The Canadian veterinary journal = La revue veterinaire canadienne 60, no. 12: 1319-1325.

Paper
Published: 23 November 2019 in Veterinary Record
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N-terminal proB-type natriuretic peptide (NT-proBNP) may be a useful marker in canine leishmaniosis (CanL). The aim was to compare NT-proBNP in dogs at different LeishVet stages of CanL and with idiopathic chronic kidney disease (CKD). Dogs diagnosed with CanL or CKD and a group of healthy dogs were included (group A, five normal dogs; group B, six dogs LeishVet 1–2; group C, 13 dogs LeishVet 3–4; group D, six dogs with CKD). NT-proBNP was higher (P<0.001) in group C (7.616 pmol/l, interquartile range (IQR) 3537–10,000 pmol/l) than in group A (293 pmol/l, IQR 257–373), group B (388.5 pmol/l, IQR 324–793) and group D (740 pmol/l, IQR 557–962 pmol/l). International Renal Interest Society (IRIS) kidney stage was not different between groups C and D or between groups A and B, but was different within all the rest of the group comparisons (P<0.001). In group C all dogs had echocardiographic increase in left ventricular mass index. NT-proBNP had negative correlation with haematocrit (P<0.001, r=0.749) and positive correlation with systemic blood pressure (P<0.001, r=0.728). NT-proBNP is consistently elevated in dogs with advanced CanL and is strongly correlated with the degree of systemic hypertension and anaemia. Moreover, dogs with advanced CanL exhibit increase in left ventricular mass. NT-proBNP may however be a less desirable cardiac marker as unlike cardiac troponin I it is often not elevated at earlier stages of CanL.

ACS Style

Domingo Casamian-Sorrosal; Rafael Barrera-Chacon; Luis Gómez; Silvia Belinchón-Lorenzo; Javier Galapero Arroyo; Jose Ignacio Cristobal; Javier Duque. Comparison of N-terminal proB-type natriuretic peptide levels at different stages of visceral leishmaniosis and in patients with chronic kidney disease. Veterinary Record 2019, 185, 630 -630.

AMA Style

Domingo Casamian-Sorrosal, Rafael Barrera-Chacon, Luis Gómez, Silvia Belinchón-Lorenzo, Javier Galapero Arroyo, Jose Ignacio Cristobal, Javier Duque. Comparison of N-terminal proB-type natriuretic peptide levels at different stages of visceral leishmaniosis and in patients with chronic kidney disease. Veterinary Record. 2019; 185 (20):630-630.

Chicago/Turabian Style

Domingo Casamian-Sorrosal; Rafael Barrera-Chacon; Luis Gómez; Silvia Belinchón-Lorenzo; Javier Galapero Arroyo; Jose Ignacio Cristobal; Javier Duque. 2019. "Comparison of N-terminal proB-type natriuretic peptide levels at different stages of visceral leishmaniosis and in patients with chronic kidney disease." Veterinary Record 185, no. 20: 630-630.

Journal article
Published: 18 September 2017 in Journal of Veterinary Internal Medicine
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Oral administration of glucocorticoid alters serum cystatin C (sCysC) concentration in humans. To determine if oral administration of prednisone alters sCysC in dogs without pre‐existing renal disease. Forty six dogs were included: 10 dogs diagnosed with steroid responsive meningitis arteritis (SRMA; group A), 20 dogs diagnosed of pituitary‐dependent hyperadrenocorticism (PDH; group B), and 16 healthy control dogs (group C). Retrospective observational study. SRMA diagnosed dogs were administered prednisone 4 mg/kg/24 h PO 7 days, reducing the dose to 2 mg/kg/24 h 7 days before medication withdrawal. In group A, sampling was performed at days 0, 7, 14 and a final control at day 21. Blood and urine samples were collected in the 3 groups, and in group A, sampling was performed at all time points (days 1, 7, 14, and 21). In group A, sCysC was significantly higher at day 7 compared to the control group (0.4 ± 0.04 mg/L vs. 0.18 ± 0.03 mg/L mean ± SEM respectively P < 0.01); sCysC values decreased to basal at day 14 when the dose was decreased and after 1 week of withdrawal of prednisone (0.27 ± 0.03 mg/L for group A at day 14 and 0.15 ± 0.02 mg/L at day 21; P > 0.05). Dogs with PDH included in group B did not have significant differences in sCysC (0.22 ± 0.03 mg/L) compared to control (P > 0.05). Oral administration of prednisone unlike altered endogenous glucocorticoid production, increases sCysC in dogs in a dose‐dependent fashion.

ACS Style

J. Muñoz; P. Soblechero; F.J. Duque; B. Macías‐García; P. Ruiz; C. Zaragoza; R. Barrera. Effects of Oral Prednisone Administration on Serum Cystatin C in Dogs. Journal of Veterinary Internal Medicine 2017, 31, 1765 -1770.

AMA Style

J. Muñoz, P. Soblechero, F.J. Duque, B. Macías‐García, P. Ruiz, C. Zaragoza, R. Barrera. Effects of Oral Prednisone Administration on Serum Cystatin C in Dogs. Journal of Veterinary Internal Medicine. 2017; 31 (6):1765-1770.

Chicago/Turabian Style

J. Muñoz; P. Soblechero; F.J. Duque; B. Macías‐García; P. Ruiz; C. Zaragoza; R. Barrera. 2017. "Effects of Oral Prednisone Administration on Serum Cystatin C in Dogs." Journal of Veterinary Internal Medicine 31, no. 6: 1765-1770.

Comparative study
Published: 01 March 2017 in The Veterinary Journal
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Canine leishmaniasis (CanL) is a systemic disease caused by the protozoan parasite Leishmania infantum. Myocarditis in CanL has been described previously in CanL by histopathological analysis of post-mortem specimens and by evaluation of cardiac troponin I (cTnI) levels. However, the degree of myocardial damage at different stages of CanL and the role that concurrent azotaemia plays in this myocardial injury are unknown. The aim of this study was to prospectively evaluate and compare the presence of myocardial injury in dogs at different stages of clinical CanL and in dogs with severe idiopathic chronic kidney disease (CKD) by measuring cTnI. Forty-eight dogs were included in the study, divided into four groups: (1) group A (10 healthy dogs); (2) group B (17 dogs with CanL without renal azotaemia, classified as mild to severe in the LeishVet scheme); (3) group C (11 dogs with CanL and renal azotaemia, classified as very severe in the LeishVet scheme); and (4) group D (10 dogs with idiopathic CKD). Dogs in group C had significantly higher cTnI than dogs in groups B and D, although cTnI was also elevated in these groups. Dogs in group A had normal cTnI values. Dogs in groups D and C had similar renal IRIS classification scorers. Severe lymphoplasmocytic myocarditis and a positive real time PCR of L. infantum DNA were observed in all dogs in group C. Dogs with very severe CanL exhibit more myocardial injury than dogs with milder CanL or dogs with idiopathic CKD.

ACS Style

L. Martínez-Hernández; Domingo Casamian-Sorrosal; R. Barrera-Chacón; J.M. Cuesta-Gerveno; S. Belinchón-Lorenzo; L.C. Gómez Nieto; F.J. Duque-Carrasco. Comparison of myocardial damage among dogs at different stages of clinical leishmaniasis and dogs with idiopathic chronic kidney disease. The Veterinary Journal 2017, 221, 1 -5.

AMA Style

L. Martínez-Hernández, Domingo Casamian-Sorrosal, R. Barrera-Chacón, J.M. Cuesta-Gerveno, S. Belinchón-Lorenzo, L.C. Gómez Nieto, F.J. Duque-Carrasco. Comparison of myocardial damage among dogs at different stages of clinical leishmaniasis and dogs with idiopathic chronic kidney disease. The Veterinary Journal. 2017; 221 ():1-5.

Chicago/Turabian Style

L. Martínez-Hernández; Domingo Casamian-Sorrosal; R. Barrera-Chacón; J.M. Cuesta-Gerveno; S. Belinchón-Lorenzo; L.C. Gómez Nieto; F.J. Duque-Carrasco. 2017. "Comparison of myocardial damage among dogs at different stages of clinical leishmaniasis and dogs with idiopathic chronic kidney disease." The Veterinary Journal 221, no. : 1-5.

Clinical trial
Published: 01 December 2015 in The Veterinary Journal
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Mesenchymal stem cells (MSCs) have shown immunomodulatory and anti-inflammatory effects in experimental colitis, and promising clinical results have been obtained in humans with Crohn's disease and ulcerative colitis. The aim of this study was to determine the safety and feasibility of adipose tissue-derived MSC (ASC) therapy in dogs with inflammatory bowel disease (IBD). Eleven dogs with confirmed IBD received one ASC intravascular (IV) infusion (2 × 10(6) cells/kg bodyweight). The outcome measures were clinical response based on percentage reduction of the validated Clinical Inflammatory Bowel Disease Activity Index (CIBDAI) and Canine Chronic Enteropathy Clinical Activity Index (CCECAI), as well as normalisation of C-reactive protein (CRP), albumin, folate and cobalamin serum concentrations at day 42 post-treatment. The Wilcoxon test was used to compare variables before and after treatment. No acute reaction to ASC infusion and no side effects were reported during follow-up in any dog. Six weeks post-treatment, the CIBDAI and CCECAI decreased significantly and albumin, cobalamin and folate concentrations increased substantially. Differences in CRP concentrations pre- and post-treatment were not significant (P = 0.050). Clinical remission (defined by a reduction of initial CIBDAI and CCECAI >75%) occurred in 9/11 dogs at day 42. The two remaining dogs showed a partial response with reduction percentages of 69.2% and 71.4%. In conclusion, a single IV infusion of allogeneic ASCs was well tolerated and appeared to produce clinical benefits in dogs with severe IBD.

ACS Style

E.M. Pérez-Merino; J.M. Usón-Casaús; C. Zaragoza-Bayle; J. Duque-Carrasco; L. Mariñas-Pardo; M. Hermida-Prieto; R. Barrera-Chacón; M. Gualtieri. Safety and efficacy of allogeneic adipose tissue-derived mesenchymal stem cells for treatment of dogs with inflammatory bowel disease: Clinical and laboratory outcomes. The Veterinary Journal 2015, 206, 385 -390.

AMA Style

E.M. Pérez-Merino, J.M. Usón-Casaús, C. Zaragoza-Bayle, J. Duque-Carrasco, L. Mariñas-Pardo, M. Hermida-Prieto, R. Barrera-Chacón, M. Gualtieri. Safety and efficacy of allogeneic adipose tissue-derived mesenchymal stem cells for treatment of dogs with inflammatory bowel disease: Clinical and laboratory outcomes. The Veterinary Journal. 2015; 206 (3):385-390.

Chicago/Turabian Style

E.M. Pérez-Merino; J.M. Usón-Casaús; C. Zaragoza-Bayle; J. Duque-Carrasco; L. Mariñas-Pardo; M. Hermida-Prieto; R. Barrera-Chacón; M. Gualtieri. 2015. "Safety and efficacy of allogeneic adipose tissue-derived mesenchymal stem cells for treatment of dogs with inflammatory bowel disease: Clinical and laboratory outcomes." The Veterinary Journal 206, no. 3: 385-390.

Clinical trial
Published: 01 December 2015 in The Veterinary Journal
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Systemic administration of mesenchymal stem cells (MSCs) has been shown to be safe and efficacious in humans with Crohn's disease. The aim of this study was to evaluate the safety of an intravenous (IV) infusion of adipose tissue-derived mesenchymal stem cells (ASCs) and to assess macroscopic and histological effects in the digestive tract of dogs with inflammatory bowel disease (IBD). Eleven dogs with confirmed IBD received a single ASC infusion (2 × 10(6) cells/kg bodyweight). Full digestive endoscopic evaluation was performed pre-treatment and between 90 and 120 days post-treatment with mucosal changes being assessed using a fit-for-purpose endoscopic scale. Endoscopic biopsies from each digestive section were evaluated histologically according to the World Small Animal Veterinary Association (WSAVA) Gastrointestinal Standardization Group criteria. The pre- and post-treatment canine IBD endoscopic index (CIBDEI) and histological score (HS) were calculated and compared using the Wilcoxon test. Remission was defined as a reduction of >75% of the CIBDEI and HS compared with pre-treatment. No acute reactions to ASC infusion or side effects were reported in any dog. Significant differences between pre- and post-treatment were found in both the CIBDEI (P = 0.004) and HS (P = 0.004). Endoscopic remission occurred in 4/11 dogs with the remaining dogs showing decreased CIBDEI (44.8% to 73.3%). Histological remission was not achieved in any dog, with an average reduction of the pre-treatment HS of 27.2%. In conclusion, a single IV infusion of allogeneic ASCs improved gastrointestinal lesions as assessed macroscopically and slightly reduced gastrointestinal inflammation as evaluated by histopathology in dogs with IBD.

ACS Style

E.M. Pérez-Merino; J.M. Usón-Casaús; J. Duque-Carrasco; C. Zaragoza-Bayle; Luis Mariñas Pardo; M. Hermida-Prieto; M. Vilafranca-Compte; R. Barrera-Chacón; Massimo Gualtieri. Safety and efficacy of allogeneic adipose tissue-derived mesenchymal stem cells for treatment of dogs with inflammatory bowel disease: Endoscopic and histological outcomes. The Veterinary Journal 2015, 206, 391 -397.

AMA Style

E.M. Pérez-Merino, J.M. Usón-Casaús, J. Duque-Carrasco, C. Zaragoza-Bayle, Luis Mariñas Pardo, M. Hermida-Prieto, M. Vilafranca-Compte, R. Barrera-Chacón, Massimo Gualtieri. Safety and efficacy of allogeneic adipose tissue-derived mesenchymal stem cells for treatment of dogs with inflammatory bowel disease: Endoscopic and histological outcomes. The Veterinary Journal. 2015; 206 (3):391-397.

Chicago/Turabian Style

E.M. Pérez-Merino; J.M. Usón-Casaús; J. Duque-Carrasco; C. Zaragoza-Bayle; Luis Mariñas Pardo; M. Hermida-Prieto; M. Vilafranca-Compte; R. Barrera-Chacón; Massimo Gualtieri. 2015. "Safety and efficacy of allogeneic adipose tissue-derived mesenchymal stem cells for treatment of dogs with inflammatory bowel disease: Endoscopic and histological outcomes." The Veterinary Journal 206, no. 3: 391-397.

Journal article
Published: 01 September 2015 in Theriogenology
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Acquired inguinal herniation is a very common condition in stallions, usually leading to unilateral or bilateral castration to prevent future recurrence. Recently, several surgical techniques such as the standing laparoscopic peritoneal flap hernioplasty (SLPFH) have been developed to avoid herniation recurrence and also preserve the breeding activity of high economic value stallions. However, studies on SLPFH lack more comprehensive and systematic data about reproductive-related adverse effects and outcomes. The aim of this study was to evaluate whether SLPFH of the internal inguinal rings produces changes in the testicular blood flow in a 1-year follow-up. For that purpose, six healthy stallions were used and testicular blood flow was assessed before, 3, 6, and 12 months (T0, T3, T6, and T12) after the procedure. Blood flow was evaluated ultrasonographically, using the pulsed-wave color Doppler mode. Peak systolic velocity, end-diastolic velocity, the time-averaged maximum velocity, and the derived indexes (resistive index) and pulsatility index) of the testicular artery were measured in two localizations: in the spermatic cord and on the caudal epididymal edge of the testicle. On the spermatic cord, the peak systolic velocity of the testicular artery increased significantly at T12. However, on the epididymal edge location of the artery, the pulsatility and resistive indexes were decreased at T12 (P < 0.05). This pattern of blood flow was related to a hyperemic process. Furthermore, SLPFH might have compressed the spermatic cord, causing a slight occlusion of the testicular artery and triggering a compensatory hyperemia to compensate the deficit of blood flow that supplies the testes. The SLPFH of the internal inguinal ring affected the testicular perfusion in stallions in a 1 year follow-up, although there was no effect on sperm production during this time. The spectral Doppler ultrasound is a useful tool to asses the testicular perfusion after reproductive surgical procedure and provides information which anticipates vascular supply compromise of the stallion testicles.

ACS Style

L.A. Gracia-Calvo; J. Duque; C. Balao Da Silva; J. Ezquerra; Cristina Ortega-Ferrusola; Francisco Javier Duque; Carolina Maria Balao Da Silva. Testicular perfusion after standing laparoscopic peritoneal flap hernioplasty in stallions. Theriogenology 2015, 84, 797 -804.

AMA Style

L.A. Gracia-Calvo, J. Duque, C. Balao Da Silva, J. Ezquerra, Cristina Ortega-Ferrusola, Francisco Javier Duque, Carolina Maria Balao Da Silva. Testicular perfusion after standing laparoscopic peritoneal flap hernioplasty in stallions. Theriogenology. 2015; 84 (5):797-804.

Chicago/Turabian Style

L.A. Gracia-Calvo; J. Duque; C. Balao Da Silva; J. Ezquerra; Cristina Ortega-Ferrusola; Francisco Javier Duque; Carolina Maria Balao Da Silva. 2015. "Testicular perfusion after standing laparoscopic peritoneal flap hernioplasty in stallions." Theriogenology 84, no. 5: 797-804.

Journal article
Published: 01 July 2015 in American Journal of Veterinary Research
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Exercise of Spanish Greyhounds without prior training activated an acute-phase response represented by an increase in serum CRP concentration and decrease in serum albumin and iron concentrations. These changes, along with leukocytosis and neutrophilia, were indicative of a subclinical inflammatory state in Spanish Greyhounds.

ACS Style

Vanesa Lucas; Rafael Barrera; Francisco J. Duque; Patricia Ruiz; Concepcion Zaragoza. Effect of exercise on serum markers of muscle inflammation in Spanish Greyhounds. American Journal of Veterinary Research 2015, 76, 637 -643.

AMA Style

Vanesa Lucas, Rafael Barrera, Francisco J. Duque, Patricia Ruiz, Concepcion Zaragoza. Effect of exercise on serum markers of muscle inflammation in Spanish Greyhounds. American Journal of Veterinary Research. 2015; 76 (7):637-643.

Chicago/Turabian Style

Vanesa Lucas; Rafael Barrera; Francisco J. Duque; Patricia Ruiz; Concepcion Zaragoza. 2015. "Effect of exercise on serum markers of muscle inflammation in Spanish Greyhounds." American Journal of Veterinary Research 76, no. 7: 637-643.

Clinical trial
Published: 28 April 2013 in Research in Veterinary Science
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The study was designed to determine the effects of two protocols of sedation, medetomidine and medetomidine-butorphanol, on cerebral blood flow (CBF) by transcranial color-coded Duplex ultrasonography in healthy dogs. Transcranial Doppler ultrasonographic examination was performed in 20 dogs before and 20 min after sedation with either medetomidine (group 1) or medetomidine-butorphanol (group 2). The left and right middle cerebral arteries (LMCA and RMCA) were evaluated using the temporal windows, and the basilar artery (BA) was studied through the suboccipital window. Peak systolic velocity (PSV), mean velocity (MV), end diastolic velocity (EDV), resistance index (RI), and pulsatility index (PI) were measured for each vessel. Blood pressure (BP) and heart rate (HR) were also recorded before and after sedation in both groups. Statistically significant differences were found for PSV, MV and EDV when RMCA and LMCA were interrogated before and after sedation. PSV, RI and PI were found to be statistically significantly different when the study was performed on the BA. These results should be taken in account when a transcranial Doppler is performed in dogs sedated with the mentioned protocols and it might suggest some degree of neuroprotection.

ACS Style

F.J. Duque; Jose-Maria Dominguez-Roldan; Fernando Martinez-Taboada; Beatriz Macias Garcia; P. Rodríguez-Medina; R. Barrera-Chacon. Effects of medetomidine and medetomidine-butorphanol on transcranial color-coded duplex ultrasonography in healthy dogs. Research in Veterinary Science 2013, 95, 648 -653.

AMA Style

F.J. Duque, Jose-Maria Dominguez-Roldan, Fernando Martinez-Taboada, Beatriz Macias Garcia, P. Rodríguez-Medina, R. Barrera-Chacon. Effects of medetomidine and medetomidine-butorphanol on transcranial color-coded duplex ultrasonography in healthy dogs. Research in Veterinary Science. 2013; 95 (2):648-653.

Chicago/Turabian Style

F.J. Duque; Jose-Maria Dominguez-Roldan; Fernando Martinez-Taboada; Beatriz Macias Garcia; P. Rodríguez-Medina; R. Barrera-Chacon. 2013. "Effects of medetomidine and medetomidine-butorphanol on transcranial color-coded duplex ultrasonography in healthy dogs." Research in Veterinary Science 95, no. 2: 648-653.

Case reports
Published: 07 October 2010 in Veterinary Radiology & Ultrasound
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We report the use of transcranial Doppler ultrasonography in a dog with hepatic encephalopathy secondary to a congenital portosystemic shunt. A severe increase in the pulsatility index was measured in the right middle cerebral artery, left middle cerebral artery, and basilar artery. These values returned to normal following medical stabilization of the patient and resolution of the neurologic signs. Transcranial Doppler ultrasonography appears to have value for monitoring the status of intracranial hypertension in patients with hepatic encephalopathy.

ACS Style

Javier Duque; Jose M. Dominguez-Roldan; Domingo Casamian-Sorrosal; Rafael Barrera-Chacon. IMAGING DIAGNOSIS-TRANSCRANIAL COLOR-CODED DUPLEX SONOGRAPHY IN A DOG WITH HEPATIC ENCEPHALOPATHY. Veterinary Radiology & Ultrasound 2010, 52, 111 -113.

AMA Style

Javier Duque, Jose M. Dominguez-Roldan, Domingo Casamian-Sorrosal, Rafael Barrera-Chacon. IMAGING DIAGNOSIS-TRANSCRANIAL COLOR-CODED DUPLEX SONOGRAPHY IN A DOG WITH HEPATIC ENCEPHALOPATHY. Veterinary Radiology & Ultrasound. 2010; 52 (1):111-113.

Chicago/Turabian Style

Javier Duque; Jose M. Dominguez-Roldan; Domingo Casamian-Sorrosal; Rafael Barrera-Chacon. 2010. "IMAGING DIAGNOSIS-TRANSCRANIAL COLOR-CODED DUPLEX SONOGRAPHY IN A DOG WITH HEPATIC ENCEPHALOPATHY." Veterinary Radiology & Ultrasound 52, no. 1: 111-113.

Journal article
Published: 25 September 2010 in Veterinary Record
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Changes in blood flow in the arteries of the canine skull base following compression of the ipsilateral carotid artery were evaluated. Forty healthy conscious dogs were evaluated during examination in lateral recumbency. Using the temporal window, the rostral, middle and caudal cerebral arteries were evaluated. The basilar artery was studied through the suboccipital window. Following compression, the pulse Doppler signal was reduced or inverted when interrogating the rostral or middle cerebral artery, and no change was observed when the caudal cerebral artery or basilar artery was evaluated.

ACS Style

F. J. Duque; R. Barrera-Chacon; P. Ruiz; Domingo Casamian-Sorrosal; C. Zaragoza; Jose-Maria Dominguez-Roldan. Effect of transient carotid artery compression during transcranial Doppler ultrasonography in dogs. Veterinary Record 2010, 167, 481 -484.

AMA Style

F. J. Duque, R. Barrera-Chacon, P. Ruiz, Domingo Casamian-Sorrosal, C. Zaragoza, Jose-Maria Dominguez-Roldan. Effect of transient carotid artery compression during transcranial Doppler ultrasonography in dogs. Veterinary Record. 2010; 167 (13):481-484.

Chicago/Turabian Style

F. J. Duque; R. Barrera-Chacon; P. Ruiz; Domingo Casamian-Sorrosal; C. Zaragoza; Jose-Maria Dominguez-Roldan. 2010. "Effect of transient carotid artery compression during transcranial Doppler ultrasonography in dogs." Veterinary Record 167, no. 13: 481-484.

Case reports
Published: 01 September 2009 in Reproduction in Domestic Animals
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In this study, two unusual presentations of canine prostatitis are described; in the first case a 10-years-old neutered Boxer dog was presented to the Veterinary Teaching Hospital of the University of Extremadura with a complaint of anorexia, apathy and preputial discharge. In the second case, a local veterinarian referred an 8-years-old male Labrador to the Veterinary Teaching Hospital of the University of Extremadura. The dog had a history of pain in the caudal abdomen and preputial oedema. The final diagnosis in both cases was acute prostatitis. It is concluded that although canine prostatitis is a common disease, sometimes can have presentations that may differ from those classically described in the literature.

ACS Style

J Duque; Beatriz Macias Garcia; P Ruíz Tapia; Cristina Ortega-Ferrusola; C Zaragoza; R Barrera; Fernando J Peña; Francisco Javier Duque. Two Unusual Cases of Canine Prostatitis: Prostatitis in a Castrated Dog and Preputial Oedema in an Intact Male. Reproduction in Domestic Animals 2009, 45, e199 -e200.

AMA Style

J Duque, Beatriz Macias Garcia, P Ruíz Tapia, Cristina Ortega-Ferrusola, C Zaragoza, R Barrera, Fernando J Peña, Francisco Javier Duque. Two Unusual Cases of Canine Prostatitis: Prostatitis in a Castrated Dog and Preputial Oedema in an Intact Male. Reproduction in Domestic Animals. 2009; 45 (5):e199-e200.

Chicago/Turabian Style

J Duque; Beatriz Macias Garcia; P Ruíz Tapia; Cristina Ortega-Ferrusola; C Zaragoza; R Barrera; Fernando J Peña; Francisco Javier Duque. 2009. "Two Unusual Cases of Canine Prostatitis: Prostatitis in a Castrated Dog and Preputial Oedema in an Intact Male." Reproduction in Domestic Animals 45, no. 5: e199-e200.

Journal article
Published: 21 August 2009 in Veterinary Radiology & Ultrasound
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Insonation of Circle of Willis by transcranial Doppler duplex color sonography is described in 30 healthy dogs with 15 weighing 33 lb. Imaging was via a temporal window to explore the rostral, middle, and caudal cerebral arteries on both the left and right‐hand sides; and through an suboccipital window to study the basilar artery. Normal mean values of the peak systolic velocity (PSV), end diastolic velocity, mean velocity, resistance index (RI), and pulsatility index (PI) were characterized and compared with those obtained in previous studies. There was significant differences in the PSV, RI, and PI in the rostral cerebral artery between dogs weighing 33 lb. Mean PSV was higher in weighing over 33 lb, whereas the mean resistive index and mean PI were lower in these dogs.

ACS Style

Francisco J. Duque; Jose-Maria Dominguez-Roldan; Patricia Ruiz; Concepcion Zaragoza; Rafael Barrera-Chacon. ASSESSING CIRCLE OF WILLIS BLOOD CIRCULATION IN DOGS WITH TRANSCRANIAL COLOR-CODED DUPLEX SONOGRAPHY. Veterinary Radiology & Ultrasound 2009, 50, 530 -535.

AMA Style

Francisco J. Duque, Jose-Maria Dominguez-Roldan, Patricia Ruiz, Concepcion Zaragoza, Rafael Barrera-Chacon. ASSESSING CIRCLE OF WILLIS BLOOD CIRCULATION IN DOGS WITH TRANSCRANIAL COLOR-CODED DUPLEX SONOGRAPHY. Veterinary Radiology & Ultrasound. 2009; 50 (5):530-535.

Chicago/Turabian Style

Francisco J. Duque; Jose-Maria Dominguez-Roldan; Patricia Ruiz; Concepcion Zaragoza; Rafael Barrera-Chacon. 2009. "ASSESSING CIRCLE OF WILLIS BLOOD CIRCULATION IN DOGS WITH TRANSCRANIAL COLOR-CODED DUPLEX SONOGRAPHY." Veterinary Radiology & Ultrasound 50, no. 5: 530-535.

Case reports
Published: 27 February 2006 in Reproduction in Domestic Animals
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A 6‐year‐old female Alaska Malamute dog was presented for evaluation of abdominal enlargement referred by a local veterinarian. On the history, the owner complained of chronic abdominal enlargement initiated more than 4 months ago, reduced appetite, occasional vomiting and general dullness. He also complained of greenish mucous intermittent vaginal discharge starting 10 days ago. The bitch was chronically treated with medroxiprogesterone acetate. A laparatomy was performed and fluid in the abdomen was found and aspirated during the surgery. Also a very fluid‐filled distended uterus and a mass in the distal part of the left uterine horn were found. The mass was encapsulated by the omentum, but areas of necrosis and calcification were identified. Histopathological diagnosis was endometrial adenocarcinoma.

ACS Style

Fernando J Peña; Ja Gines; Francisco Javier Duque; V Vieitez; R Martinez -Perez; L Madejón; I Nunez Martinez; José M. Morán; S Fernandez-Garcia. Endometrial Adenocarcinoma and Mucometra in a 6-year-old Alaska Malamute Dog. Reproduction in Domestic Animals 2006, 41, 189 -190.

AMA Style

Fernando J Peña, Ja Gines, Francisco Javier Duque, V Vieitez, R Martinez -Perez, L Madejón, I Nunez Martinez, José M. Morán, S Fernandez-Garcia. Endometrial Adenocarcinoma and Mucometra in a 6-year-old Alaska Malamute Dog. Reproduction in Domestic Animals. 2006; 41 (2):189-190.

Chicago/Turabian Style

Fernando J Peña; Ja Gines; Francisco Javier Duque; V Vieitez; R Martinez -Perez; L Madejón; I Nunez Martinez; José M. Morán; S Fernandez-Garcia. 2006. "Endometrial Adenocarcinoma and Mucometra in a 6-year-old Alaska Malamute Dog." Reproduction in Domestic Animals 41, no. 2: 189-190.