This page has only limited features, please log in for full access.
Scientist at the department chemistry and biochemistry Mendel University Brno https://orcid.org/0000-0003-2836-022X
Knowledge of the concentration of the bioavailable forms of mercury in the soil is necessary, especially, if these soils contain above-limit total mercury concentrations. The bioavailability of mercury in soil samples collected from the vicinity of abandoned cinnabar mines was evaluated using diffusive gradients in the thin films technique (DGT) and mercury phytoaccumulation by vegetables (lettuce, spinach, radish, beetroot, carrot, and green peas). Mercury was accumulated primarily in roots of vegetables. The phytoaccumulation of mercury into edible plant parts was site-specific as well as vegetable species-specific. The mercury concentration in edible parts decreased in the order: spinach leaf ≥ lettuce leaf ≥ carrot storage root ≥ beetroot storage root > radish storage root > pea legume. The translocation index as well as the target hazard quotient indicate the possible usability of soils from the vicinity of abandoned cinnabar mines for planting pod vegetables (peas). A strong positive correlation (r = 0.75 to 0.92) was observed between mercury concentration in secondary roots, the storage roots, leaves of vegetables and the flux of mercury from soil to the DGT units, and the effective concentration of mercury in soil solutions.
Pavlína Pelcová; Andrea Ridošková; Jana Hrachovinová; Jan Grmela. Evaluation of mercury bioavailability to vegetables in the vicinity of cinnabar mine. Environmental Pollution 2021, 283, 117092 .
AMA StylePavlína Pelcová, Andrea Ridošková, Jana Hrachovinová, Jan Grmela. Evaluation of mercury bioavailability to vegetables in the vicinity of cinnabar mine. Environmental Pollution. 2021; 283 ():117092.
Chicago/Turabian StylePavlína Pelcová; Andrea Ridošková; Jana Hrachovinová; Jan Grmela. 2021. "Evaluation of mercury bioavailability to vegetables in the vicinity of cinnabar mine." Environmental Pollution 283, no. : 117092.
Selenium nanoparticles (SeNPs) are fast becoming a key instrument in several applications such as medicine or nutrition. Questions have been raised about the safety of their use. Male rats were fed for 28 days on a monodiet containing 0.5, 1.5, 3.0 and 5.0 mg Se/kg. Se content in blood and liver, liver panel tests, blood glucose, total antioxidant capacity (TAC), the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) were analysed. Liver and duodenum were subjected to histopathology examination. The weight gain of rats showed no differences between tested groups. Se content in blood was higher in all treated groups compared to the control group. The liver concentration of Se in the treated groups varied in the range from 222 to 238 ng/g. No differences were observed in the activity of AST (aspartate aminotransferase), ALP (alkaline phosphatase) and TAS (total antioxidant status). A significant decrease in ALT activity compared to the control group was observed in the treated groups. GPx activity varied from 80 to 88 U/mL through tested groups. SOD activity in liver was decreased in the SeNP-treated group with 5 mg Se/kg (929 ± 103 U/mL). Histopathological examination showed damage to the liver parenchyma and intestinal epithelium in a dose-dependent manner. This study suggests that short-term SeNP supplementation can be safe and beneficial in Se deficiency or specific treatment.
Lenka Urbankova; Sylvie Skalickova; Magdalena Pribilova; Andrea Ridoskova; Pavlina Pelcova; Jiri Skladanka; Pavel Horky. Effects of Sub-Lethal Doses of Selenium Nanoparticles on the Health Status of Rats. Toxics 2021, 9, 28 .
AMA StyleLenka Urbankova, Sylvie Skalickova, Magdalena Pribilova, Andrea Ridoskova, Pavlina Pelcova, Jiri Skladanka, Pavel Horky. Effects of Sub-Lethal Doses of Selenium Nanoparticles on the Health Status of Rats. Toxics. 2021; 9 (2):28.
Chicago/Turabian StyleLenka Urbankova; Sylvie Skalickova; Magdalena Pribilova; Andrea Ridoskova; Pavlina Pelcova; Jiri Skladanka; Pavel Horky. 2021. "Effects of Sub-Lethal Doses of Selenium Nanoparticles on the Health Status of Rats." Toxics 9, no. 2: 28.
Knowledge of the fractionation of mercury in soils in the vicinity of abandoned cinnabar mines is essential for assessing the usability of soils for the cultivation of agriculturally important crops. Two different sequential extraction methods and the technique of diffusive gradients in thin films (DGT) were applied and compared for fractionation of mercury in soils from mercury‐contaminated sites intended for farming purposes. The mercury found in these soils was primarily in the form of mercury sulphide (58.6 to 83.9 %), followed by 6.7 to 15.4 % of organically bound mercury, and 2.9 to 23.2 % of elemental mercury. Up to 10.3 % of labile mercury species were determined by both sequential extraction methods in these soils. However, only 0.01 to 0.13 % of mercury was determined as a bioavailable fraction using the DGT technique. Both sequential extraction methods tested for the fractionation analysis of mercury in contaminated soils were in excellent agreement. The content of the mobile (labile) mercury determined by the sequential extraction methods was statistically significantly higher (p < 0.0001) than the content of bioavailable mercury determined by the DGT technique. This article is protected by copyright. All rights reserved.
Pavlína Pelcová; Andrea Ridošková; Jana Hrachovinová; Jan Grmela. Fractionation Analysis of Mercury in Soils: A Comparison of Three Techniques for Bioavailable Mercury Fraction Determination. Environmental Toxicology and Chemistry 2020, 39, 1670 -1677.
AMA StylePavlína Pelcová, Andrea Ridošková, Jana Hrachovinová, Jan Grmela. Fractionation Analysis of Mercury in Soils: A Comparison of Three Techniques for Bioavailable Mercury Fraction Determination. Environmental Toxicology and Chemistry. 2020; 39 (9):1670-1677.
Chicago/Turabian StylePavlína Pelcová; Andrea Ridošková; Jana Hrachovinová; Jan Grmela. 2020. "Fractionation Analysis of Mercury in Soils: A Comparison of Three Techniques for Bioavailable Mercury Fraction Determination." Environmental Toxicology and Chemistry 39, no. 9: 1670-1677.
An ion-exchange resin Lewatit FO 36 was used for the preparation of a new resin gel for the diffusive gradient in thin films technique (DGT). The DGT method was optimized for the accumulation of four bioavailable arsenic species (AsIII, AsV, monomethylarsonic acid, dimethylarsinic acid) in the aquatic environment. The total sorption capacity of Lewatit FO 36 resin gel was 535 μg As disc−1. The microwave-assisted extraction in the presence of NaCl (10 g L−1) and NaOH (10 g L−1) was used for the isolation of arsenic species from the Lewatit FO 36 resin gel. The elution efficiency of arsenic was 98.4 ± 2.0%. Arsenic was determined by the optimized electrothermal atomic absorption spectrometry (ET-AAS) method using palladium modifier, pre-atomization cool-down step and tungsten carbides coating of graphite tube. The Lewatit FO 36 resin gel provides accurate results (cDGT/cSOL ratio 0.86–1.00) in the pH range 4–8. No significant influence of experimental conditions was observed in the presence of chlorides (0–0.5 mol L−1) and humic acid (0–100 mg L−1). Only a very high concentration of phosphates (10 mg L−1) caused a slight decrease in the diffusion coefficients of MMA and AsV species (8.4% and 12.4%, respectively). The presence of iron (0–1 mg L−1) caused a decrease in the diffusion coefficients, but with regard to the common concentrations of iron (less than 0.3 mg L−1), the negative effect was considered not significant for AsIII and DMA in natural water. The DGT-ET-AAS method was applied for the determination of bioavailable arsenic species in the spiked river water samples and also in-situ in the water reservoir. The new resin gel was characterized by a homogeneous gel structure with excellent reproducibility (< 5% variation of results between batches) and high sorption capacity which suggests its possible long-term application (up to 286 days in the environment with the arsenic concentration of 100 μg L−1).
Vendula Smolikova; Pavlína Pelcová; Andrea Ridošková; Josef Hedbavny; Jan Grmela. Development and evaluation of the iron oxide-hydroxide based resin gel for the diffusive gradient in thin films technique. Analytica Chimica Acta 2019, 1102, 36 -45.
AMA StyleVendula Smolikova, Pavlína Pelcová, Andrea Ridošková, Josef Hedbavny, Jan Grmela. Development and evaluation of the iron oxide-hydroxide based resin gel for the diffusive gradient in thin films technique. Analytica Chimica Acta. 2019; 1102 ():36-45.
Chicago/Turabian StyleVendula Smolikova; Pavlína Pelcová; Andrea Ridošková; Josef Hedbavny; Jan Grmela. 2019. "Development and evaluation of the iron oxide-hydroxide based resin gel for the diffusive gradient in thin films technique." Analytica Chimica Acta 1102, no. : 36-45.
The diffusive gradients in thin films technique (DGT) was used for the determination of bioavailable mercury in urban soils, and results were compared to the mercury accumulation by Pisum sativum L. (pea) part (leaf, root, stem, blossom, legume, and green seed). The total mercury concentration in soil samples was ranged between 0.084 - 0.326 mg kg-1. The soil solutions contained 0.15% to 0.20% of mercury present in soils. In the soil solution, 2.21% to 3.45% of mercury was available for DGT units. The highest mercury content was determined in the leaf and root of the pea plant, and the lowest in the consumable part of the pea plant (green seed). Mercury concentration in the parts of the pea plant increased over time, alongside the growth of the plant. The effect of acid precipitation on mercury bioavailability was statistically non-significant (p = 0.53). Significant and positive correlations were found between mercury flux into DGT unit, and mercury flux into the root (r = 0.989), leaf (r = 0.985), and stem (r = 0.904) of the pea plant. The obtained results suggest that the DGT method could be used for description of the uptake of mercury by pea plant parts in non-contaminated and slightly contaminated soils.
Pavlína Pelcová; Iveta Zouharová; Andrea Ridošková; Vendula Smolikova. Evaluation of mercury availability to pea parts (Pisum sativum L.) in urban soils: Comparison between diffusive gradients in thin films technique and plant model. Chemosphere 2019, 234, 373 -378.
AMA StylePavlína Pelcová, Iveta Zouharová, Andrea Ridošková, Vendula Smolikova. Evaluation of mercury availability to pea parts (Pisum sativum L.) in urban soils: Comparison between diffusive gradients in thin films technique and plant model. Chemosphere. 2019; 234 ():373-378.
Chicago/Turabian StylePavlína Pelcová; Iveta Zouharová; Andrea Ridošková; Vendula Smolikova. 2019. "Evaluation of mercury availability to pea parts (Pisum sativum L.) in urban soils: Comparison between diffusive gradients in thin films technique and plant model." Chemosphere 234, no. : 373-378.
We assessed the relationship between the diffusive gradient in thin film (DGT) technique using the new ion‐exchange resin Ambersep GT74 and the uptake of mercury (Hg) by a model plant cultivated on metal‐contaminated agricultural soils under greenhouse conditions. Based on the total Hg content, 0.37 to 1.17% of the Hg passed to the soil porewater from the solid phase, and 2.18 to 9.18% of the Hg is DGT‐available. These results were confirmed by calculating the R value (the ratio of the concentrations of bioavailable Hg measured by DGT and soil solution), which illustrated the strong bonding of Hg to the solid phase of soil and its extremely low mobility. Only inorganic Hg2+ species were found in the metal‐contaminated agricultural soils, as determined by a high‐performance liquid chromatography–cold vapor atomic fluorescence spectrometry speciation analysis. The Hg was distributed in Miscanthus × giganteus organs in the following order for all sampling sites: roots (55–82%) >> leaves (8–27%) > stems (7–16%) > rhizomes (4–7%). Environ Toxicol Chem 2019;9999:1–8. © 2018 SETAC
Andrea Ridošková; Aurélie Pelfrêne; Francis Douay; Pavlína Pelcová; Vendula Smolikova; Vojtěch Adam. Bioavailability of mercury in contaminated soils assessed by the diffusive gradient in thin film technique in relation to uptake by Miscanthus × giganteus. Environmental Toxicology and Chemistry 2019, 38, 321 -328.
AMA StyleAndrea Ridošková, Aurélie Pelfrêne, Francis Douay, Pavlína Pelcová, Vendula Smolikova, Vojtěch Adam. Bioavailability of mercury in contaminated soils assessed by the diffusive gradient in thin film technique in relation to uptake by Miscanthus × giganteus. Environmental Toxicology and Chemistry. 2019; 38 (2):321-328.
Chicago/Turabian StyleAndrea Ridošková; Aurélie Pelfrêne; Francis Douay; Pavlína Pelcová; Vendula Smolikova; Vojtěch Adam. 2019. "Bioavailability of mercury in contaminated soils assessed by the diffusive gradient in thin film technique in relation to uptake by Miscanthus × giganteus." Environmental Toxicology and Chemistry 38, no. 2: 321-328.
The ability of the DGT technique to predict Hg2+ bioavailability for input tissues (skin, gills, and scales) of common carp in the presence of chloride ions and humic acid (HA) was evaluated. The mercury accumulation by the DGT units and input tissues of carp decreased with an increasing concentration of chloride ions (29-180 mg L-1) and HA (0-5 mg L-1). In the presence of chloride ions and HA, statistically significant correlations (Pearson’s correlation coefficients 0.731-0.954) were determined between the rate of mercury accumulation by input tissues of carp and the rate of mercury accumulation by the DGT units. The laboratory experiments suggest the possibility of using the DGT technique for predicting the mercury bioaccumulation in natural aquatic ecosystems instead of commonly used input tissues of fish.
Pavlína Pelcová; Petra Vičarová; Hana Dočekalová; Eva Poštulková; Radovan Kopp; Jan Mareš; Vendula Smolíková. The prediction of mercury bioavailability for common carp (Cyprinus carpio L.) using the DGT technique in the presence of chloride ions and humic acid. Chemosphere 2018, 211, 1109 -1112.
AMA StylePavlína Pelcová, Petra Vičarová, Hana Dočekalová, Eva Poštulková, Radovan Kopp, Jan Mareš, Vendula Smolíková. The prediction of mercury bioavailability for common carp (Cyprinus carpio L.) using the DGT technique in the presence of chloride ions and humic acid. Chemosphere. 2018; 211 ():1109-1112.
Chicago/Turabian StylePavlína Pelcová; Petra Vičarová; Hana Dočekalová; Eva Poštulková; Radovan Kopp; Jan Mareš; Vendula Smolíková. 2018. "The prediction of mercury bioavailability for common carp (Cyprinus carpio L.) using the DGT technique in the presence of chloride ions and humic acid." Chemosphere 211, no. : 1109-1112.
In natural waters and in breeding tanks certain substances are naturally present in the water or deliberately added to it. Some of these substances are characterized by the formation of strong complexes with mercury and thus they can reduce the intake of mercury by aquatic organisms. The accumulation of mercury in six selected tissues (skin, scales, kidneys, muscle, liver and gills) of Cyprinus carpio L. in the presence of humic acid and sodium chloride was studied. Carp fingerlings were exposed in fish tanks for 3 days to the bulk solution containing a defined concentration of Hg2+ (1.5 μg/l) and increasing concentration of humic acid (0–5 mg/l) and chloride ions (18–600 mg/l). The fish were not fed during the experiment and mercury accumulated in the carp tissues from fish tank water only. Total mercury content in water and in selected tissues was determined by the atomic absorption spectrometer AMA 254. Increased concentration of both humic acid and sodium chloride caused reduction of mercury accumulation in carp tissues up to 93 and 45%, respectively.
Petra Vičarová; Hana Dočekalová; Eva Poštulková; Jan Mareš; Radovan Kopp; Pavlína Pelcová. Reduction of Mercury Accumulation in Carp Tissues (Cyprinus carpio L.) by Natural Organic Matter and Chlorides. International Journal of Environmental Research 2018, 12, 127 -133.
AMA StylePetra Vičarová, Hana Dočekalová, Eva Poštulková, Jan Mareš, Radovan Kopp, Pavlína Pelcová. Reduction of Mercury Accumulation in Carp Tissues (Cyprinus carpio L.) by Natural Organic Matter and Chlorides. International Journal of Environmental Research. 2018; 12 (2):127-133.
Chicago/Turabian StylePetra Vičarová; Hana Dočekalová; Eva Poštulková; Jan Mareš; Radovan Kopp; Pavlína Pelcová. 2018. "Reduction of Mercury Accumulation in Carp Tissues (Cyprinus carpio L.) by Natural Organic Matter and Chlorides." International Journal of Environmental Research 12, no. 2: 127-133.
The mercury bioaccumulation by common carp (Cyprinus carpio L.) tissues (gills, skin, eyes, scales, muscle, brain, kidneys, liver, and spleen) and the capability of the diffusive gradient in thin film (DGT) technique to predict bioavailability of mercury for individual carp's tissues were evaluated. Carp and DGT units were exposed to increasing concentrations of mercury (Hg(2+): 0 μg L(-1), 0.5 μg L(-1), 1.5 μg L(-1) and 3.0 μg L(-1)) in fish tanks for 14 days. In the uncontaminated fish group, the highest mercury concentration was determined in the muscle tissues and, in fish groups exposed to mercury, the highest mercury concentration was determined in the detoxification (kidneys) and input (gills) organs. A strong and positive correlation between the rate of mercury uptake by the DGT technique and the rate of mercury accumulation by fish tissues (gills, skin, scales, and eyes) was observed.
Pavlína Pelcová; Petra Vičarová; Andrea Ridošková; Hana Dočekalová; Radovan Kopp; Jan Mareš; Eva Poštulková. Prediction of mercury bioavailability to common carp (Cyprinus carpio L.) using the diffusive gradient in thin film technique. Chemosphere 2017, 187, 181 -187.
AMA StylePavlína Pelcová, Petra Vičarová, Andrea Ridošková, Hana Dočekalová, Radovan Kopp, Jan Mareš, Eva Poštulková. Prediction of mercury bioavailability to common carp (Cyprinus carpio L.) using the diffusive gradient in thin film technique. Chemosphere. 2017; 187 ():181-187.
Chicago/Turabian StylePavlína Pelcová; Petra Vičarová; Andrea Ridošková; Hana Dočekalová; Radovan Kopp; Jan Mareš; Eva Poštulková. 2017. "Prediction of mercury bioavailability to common carp (Cyprinus carpio L.) using the diffusive gradient in thin film technique." Chemosphere 187, no. : 181-187.
Heavy metal pollution is an important concern because of its potential to affect human health. This study was conducted to analyze plants growing on a landfill body and in its surroundings to determine their potential for heavy metal accumulation. In addition, the enrichment coefficient (EC) for the plant/soil system was used for determining the environmental contamination from a landfill in terms of heavy metal accumulation. The samples were taken in 2013-2014. Of the analyzed metals, iron achieved the highest values in the samples, i.e. - stalk (103.4-6564.6 mg/kg DM), roots (6563.6-33,036.6 mg/kg DM), leaf (535.1-11,275 mg/kg DM) and soil (12,389-39,381.9 mg/kg DM). The highest concentrations were determined in 2013 for Fe, Mn and Zn. Iron achieved the highest concentrations in the years 2013-2014. Next, EC values were then calculated, with the highest noted for Cd. Cd, as well as Cr, Ni and Zn are accumulated mostly in the leaves, whereas Co, Cu, Fe, Hg, Mn and Pb are accumulated mainly in the roots of T. vulgare.
Dana Adamcová; Maja Radziemska; Andrea Ridošková; Stanislav Barton; Pavlína Pelcová; Jakub Elbl; Jindřich Kynický; Martin Brtnický; Magdalena Daria Vaverková. Environmental assessment of the effects of a municipal landfill on the content and distribution of heavy metals in Tanacetum vulgare L. Chemosphere 2017, 185, 1011 -1018.
AMA StyleDana Adamcová, Maja Radziemska, Andrea Ridošková, Stanislav Barton, Pavlína Pelcová, Jakub Elbl, Jindřich Kynický, Martin Brtnický, Magdalena Daria Vaverková. Environmental assessment of the effects of a municipal landfill on the content and distribution of heavy metals in Tanacetum vulgare L. Chemosphere. 2017; 185 ():1011-1018.
Chicago/Turabian StyleDana Adamcová; Maja Radziemska; Andrea Ridošková; Stanislav Barton; Pavlína Pelcová; Jakub Elbl; Jindřich Kynický; Martin Brtnický; Magdalena Daria Vaverková. 2017. "Environmental assessment of the effects of a municipal landfill on the content and distribution of heavy metals in Tanacetum vulgare L." Chemosphere 185, no. : 1011-1018.
A diffusive gradient in thin films technique (DGT) was combined with liquid chromatography (LC) and cold vapor atomic fluorescence spectrometry (CV-AFS) for the simultaneous quantification of four mercury species (Hg(2+), CH3Hg(+), C2H5Hg(+), and C6H5Hg(+)). After diffusion through an agarose diffusive layer, the mercury species were accumulated in resin gels containing thiol-functionalized ion-exchange resins (Duolite GT73, and Ambersep GT74). A microwave-assisted extraction (MAE) in the presence of 6M HCl and 5 M HCl (55 °C, 15 min) was used for isolation of mercury species from Ambersep and Duolite resin gels, respectively. The extraction efficiency was higher than 95.0% (RSD 3.5%). The mercury species were separated with a mobile phase containing 6.2% methanol+0.05% 2-mercaptoethanol+0.02 M ammonium acetate with a stepwise increase of methanol content up to 80% in the 16th min on a Zorbax C18 reverse phase column. The LODs of DGT-MAE-LC-CV-AFS method were 38 ng L(-1) for CH3Hg(+), 13 ng L(-1) for Hg(2+), 34 ng L(-1) for C2H5Hg(+) and 30 ng L(-1) for C6H5Hg(+) for 24 h DGT accumulation at 25 °C.
Pavlína Pelcová; Hana Dočekalová; Andrea Kleckerová. Determination of mercury species by the diffusive gradient in thin film technique and liquid chromatography – atomic fluorescence spectrometry after microwave extraction. Analytica Chimica Acta 2015, 866, 21 -26.
AMA StylePavlína Pelcová, Hana Dočekalová, Andrea Kleckerová. Determination of mercury species by the diffusive gradient in thin film technique and liquid chromatography – atomic fluorescence spectrometry after microwave extraction. Analytica Chimica Acta. 2015; 866 ():21-26.
Chicago/Turabian StylePavlína Pelcová; Hana Dočekalová; Andrea Kleckerová. 2015. "Determination of mercury species by the diffusive gradient in thin film technique and liquid chromatography – atomic fluorescence spectrometry after microwave extraction." Analytica Chimica Acta 866, no. : 21-26.
The diffusive gradients in thin films (DGT) technique, utilizing resin gel with ion-exchange resin Duolite GT73 and new ion-exchange resin Ambersep GT74, was investigated for the accumulation of four mercury species (Hg(2+), CH3Hg(+), C2H5Hg(+), C6H5Hg(+)). The diffusion coefficients of mercury species in agarose gel calculated on the basis of Fick's Law were mercury species-specific. The diffusion coefficients of Hg(2+) and CH3Hg(+) at 25 °C (9.07±0.23×10(-6) cm(2) s(-1) and 9.06±0.30×10(-6) cm(2) s(-1), respectively) were very similar, but the diffusion coefficients of C2H5Hg(+) (6.87±0.23×10(-6) cm(2) s(-1)) and C6H5Hg(+) (3.86±0.19×10(-6) cm(2) s(-1)) were significantly lower. Influence of experimental conditions (pH, selected cations, chlorides and humic substance) on mercury species accumulation by DGT was studied. The DGT technique was applied to river water spiked with mercury species.
Pavlína Pelcová; Hana Dočekalová; Andrea Kleckerová. Development of the diffusive gradient in thin films technique for the measurement of labile mercury species in waters. Analytica Chimica Acta 2014, 819, 42 -48.
AMA StylePavlína Pelcová, Hana Dočekalová, Andrea Kleckerová. Development of the diffusive gradient in thin films technique for the measurement of labile mercury species in waters. Analytica Chimica Acta. 2014; 819 ():42-48.
Chicago/Turabian StylePavlína Pelcová; Hana Dočekalová; Andrea Kleckerová. 2014. "Development of the diffusive gradient in thin films technique for the measurement of labile mercury species in waters." Analytica Chimica Acta 819, no. : 42-48.
The molecularly imprinted SPE directly coupled to RP LC‐MS/MS method has been developed and successfully validated for the determination of six hormones in water and sediment samples. The method is based on the use the home‐made column filled with a molecularly imprinted sorbent (imprinted against estrogens) that was used under nonaqueous conditions. Thus, its high selectivity could be utilized resulting in low matrix components’ coextraction. The method showed excellent recovery (92–105%) and satisfactory sensitivity (LOQs water: 1.9–4.0 ng/L; LOQs sediment: 0.2–0.5 ng/g). The intra‐ and interprecision for water and sediment was in the range of 4.0–6.0% and 4.4–7.6%, respectively. Finally, 20 water and sediment samples collected from the Svratka river were analyzed. Only estrone was quantified in eight water samples (4.4–7.1 ng/L); no analytes were found in sediment samples.
David Matějíček; Jiri Vlcek; Alena Buresová; Pavlína Pelcová. Online molecularly imprinted solid-phase extraction coupled to liquid chromatography-tandem mass spectrometry for the determination of hormones in water and sediment samples. Journal of Separation Science 2013, 36, 1509 -1515.
AMA StyleDavid Matějíček, Jiri Vlcek, Alena Buresová, Pavlína Pelcová. Online molecularly imprinted solid-phase extraction coupled to liquid chromatography-tandem mass spectrometry for the determination of hormones in water and sediment samples. Journal of Separation Science. 2013; 36 (9):1509-1515.
Chicago/Turabian StyleDavid Matějíček; Jiri Vlcek; Alena Buresová; Pavlína Pelcová. 2013. "Online molecularly imprinted solid-phase extraction coupled to liquid chromatography-tandem mass spectrometry for the determination of hormones in water and sediment samples." Journal of Separation Science 36, no. 9: 1509-1515.
Andrea Ridoskova; Hana Dočekalová; Pavlína Pelcová. Prediction of cadmium, lead and mercury availability to plants: comparison between diffusive gradient in thin films technique measurement and soil grown plants. Journal of Elementology 2012, 1 .
AMA StyleAndrea Ridoskova, Hana Dočekalová, Pavlína Pelcová. Prediction of cadmium, lead and mercury availability to plants: comparison between diffusive gradient in thin films technique measurement and soil grown plants. Journal of Elementology. 2012; (1/2017):1.
Chicago/Turabian StyleAndrea Ridoskova; Hana Dočekalová; Pavlína Pelcová. 2012. "Prediction of cadmium, lead and mercury availability to plants: comparison between diffusive gradient in thin films technique measurement and soil grown plants." Journal of Elementology , no. 1/2017: 1.
Basic operation principles of a lightweight, low power, low cost, portable ion chromatograph utilizing open tubular ion chromatography in capillary columns coated with multi-layer polymeric stationary phases are demonstrated. A minimalistic configuration of a portable IC instrument was developed that does not require any chromatographic eluent delivery system, nor sample injection device as it uses gravity-based eluent flow and hydrodynamic sample injection adopted from capillary electrophoresis. As a detection device, an inexpensive commercially available capacitance sensor is used that has been shown to be a suitable substitute for contactless conductivity detection in capillary separation systems. The built-in temperature sensor allows for baseline drift correction typically encountered in conductivity/capacitance measurements without thermostating device. The whole instrument does not require any power supply for its operation, except the detection and data acquisition part that is provided by a USB port of a Netbook computer. It is extremely lightweight, its total weight including the Netbook computer is less than 2.5 kg and it can be continuously operated for more than 8 h. Several parameters of the instrument, such as detection cell design, eluent delivery systems and data treatment were optimized as well as the composition of eluent for non-suppressed ion chromatographic analysis of common inorganic cations (Na + , NH 4 + , K + , Cs + , Ca 2+ , Mg 2+ , transition metals). Low conductivity eluents based on weakly complexing organic acids such as tartaric, oxalic or pyridine-2,6-dicarboxylic acids were used with contactless capacitance detection for simultaneous separation of mono- and divalent cations. Separation of Na + and NH 4 + cations was optimized by addition of 18-crown-6 to the eluent. The best separation of 6 metal cations commonly present in various environmental samples was accomplished in less than 30 min using a 1.75 mM pyridine-2,6-dicarboxylic acid and 3 mM 18-crown-6 eluent with excellent repeatability (below 2%) and detection limits in the low micromolar range. The analysis of field samples is demonstrated; the concentrations of common inorganic cations in river water, mineral water and snow samples were determined. Keywords Portable ion chromatograph Open tubular ion chromatography Gravity flow Alkali and alkaline earth cations Contactless capacitance detection 1 Introduction Among current trends in analytical chemistry towards miniaturization [1] and use of green chemistry [2] , development of portable instrumentation has seen growth of interest in recent years and has been subject to several extensive reviews [3–6] and a recently published book [7] . Accurate, fast and reliable on-site determination of various compounds ranging from warfare agents [8,9] , explosive residuals [10,11] to the complex analysis of environmental samples [12] in national protection areas or hazardous waste disposal sites has become important. Portable instruments can provide instant results without the need to wait for the transport, analysis and data evaluation from a remote analytical laboratory. Although several simple, portable instruments are commercially available, they typically measure only one physicochemical property at a time, i.e. pH, absorbance, conductivity, optical properties, mass spectra, etc. This is seldom satisfactory since a separation of multiple analyte species is often required. Development of a portable separation system is thus a logical step in the research area dealing with portable instrumentation. General requirements for a field-portable instrument are the following: low weight (to be carried by a single person into remote areas), low power consumption, robustness, reliability, multianalyte determination capability and analytical performance similar to laboratory instruments. The instrument should also be relatively insensitive to temperature and humidity variations that may occur under field measurements in open space. Liquid chromatographic separation methods that provide multianalyte determination capabilities and that are very powerful in research laboratories are not quite easily transferred into a field-portable format, except gas chromatography (GC) [13–16] . Gas chromatographs are at present commonly available in a portable format from several vendors (see for instance [17] ). This is due to the fact that small gas cylinders with gaseous mobile phase can be easily used and carried along with the instrument. In liquid chromatographic methods, i.e. LC or IC, some difficulties, such as the need to deliver eluents at high pressures and use of bulky detection methods (UV–vis, MS, etc.) resulting in large size/weight and high power consumption present a serious hindrance in the development of a simple, lightweight portable LC or IC instrument. Although there were some attempts to develop portable liquid chromatographs, the number of papers is rather limited. Baram [18] has discussed some basic requirements and limitations for successful construction of a portable LC system. They have constructed a portable LC system that weighed ca. 15 kg and has been tested in a mobile laboratory where the electric power and batteries could easily be accommodated. The portability of such instrument into remote areas may be difficult. Boring et al. [19] have presented a portable IC instrument based on packed capillary ion chromatographic columns, a small size syringe pump and conductometric detection. Kalyakina and Dolgonosov [20] have presented another design of a portable IC instrument. In both cases the weight of such an instrument was not less than 10 kg and typical operation times of several hours could be achieved. Tanaka et al. [21] have used a commercially available ion chromatograph (Shimadzu PIA-1000, www.shimadzu.eu ) that was operated using a car battery for monitoring acid rain. The instrument itself weighs 15 kg, excluding the car...
Isaac K. Kiplagat; Petr Kubáň; Pavlína Pelcová; Vlastimil Kubáň. Portable, lightweight, low power, ion chromatographic system with open tubular capillary columns. Journal of Chromatography A 2010, 1217, 5116 -5123.
AMA StyleIsaac K. Kiplagat, Petr Kubáň, Pavlína Pelcová, Vlastimil Kubáň. Portable, lightweight, low power, ion chromatographic system with open tubular capillary columns. Journal of Chromatography A. 2010; 1217 (31):5116-5123.
Chicago/Turabian StyleIsaac K. Kiplagat; Petr Kubáň; Pavlína Pelcová; Vlastimil Kubáň. 2010. "Portable, lightweight, low power, ion chromatographic system with open tubular capillary columns." Journal of Chromatography A 1217, no. 31: 5116-5123.
Abiotic parameters (pH, temperature, current velocity, mercury species concentration, and sediment and aqueous media composition) influence mercury species (MeHg+, EtHg+, PhHg+ and inorganic Hg2+) adsorption on river sediments. The highest amount of adsorbed MeHg+ and EtHg+ (82–93% and 85–91% for static and agitated system, respectively) occurred at pH 3–4. For PhHg+ the maximum adsorption (90% and 95% for static and agitated systems) was located over the broad 3–10 pH range, while for Hg2+ (94% and 97% for static and agitated systems) it was at pH ∼ 3. Temperature (4.5–60°C) influenced the adsorption rate but not the quantity. Both rate and quantity increased in the order: static 2+ adsorption about 67%. Cations at pH 5.2 reduced either the adsorption rate (Ca2+, Al3+) or the total adsorption (Zn2+, Fe3+). Positive correlations were found between sediment C, N, S content as well as cation exchange capacity (CEC) with mercury adsorption (R = 0.45–0.66, 0.56–0.89, 0.45–0.61 and 0.55–0.73, respectively) while negative correlations were observed with Fe and Al (R = −0.63 to −0.90 and −0.65 to −0.86, respectively).
Pavlína Pelcová; Jana Margetínová; Tomas Vaculovic; Josef Komárek; Vlastimil Kubáň. Adsorption of mercury species on river sediments — effects of selected abiotic parameters. Open Chemistry 2010, 8, 116 -125.
AMA StylePavlína Pelcová, Jana Margetínová, Tomas Vaculovic, Josef Komárek, Vlastimil Kubáň. Adsorption of mercury species on river sediments — effects of selected abiotic parameters. Open Chemistry. 2010; 8 (1):116-125.
Chicago/Turabian StylePavlína Pelcová; Jana Margetínová; Tomas Vaculovic; Josef Komárek; Vlastimil Kubáň. 2010. "Adsorption of mercury species on river sediments — effects of selected abiotic parameters." Open Chemistry 8, no. 1: 116-125.
Polyelectrolyte multilayers deposited on the wall of fused silica capillaries were used as stationary phases in open tubular ion chromatography. The multilayers were formed by flushing the capillaries with solutions of polyanions and polycations such as polydiallyldimethylammonium chloride and dextran sulphate. Columns with several bi-layers were constructed and used in low pressure non-suppressed open tubular ion chromatography of common inorganic anions (F(-), Cl(-), NO(3)(-)) and cations (Li(+), Na(+), NH(4)(+), K(+), Cs(+)) with contactless conductometric detection. Using sodium benzoate and tartaric acid eluents the separations were typically achieved in less than 35 min with separation efficiencies between 2000 and 9000 theoretical plates. A bi-functional column was prepared that contains both anionic and cationic functional groups and was used for simultaneous separation of anions and cations.
Pavlína Pelcová; Vlastimil Kubáň; Isaac K. Kiplagat; Petr Kubáň. Polyelectrolyte multilayers as stationary phases for open tubular ion chromatography. Journal of Chromatography A 2009, 1216, 9022 -9026.
AMA StylePavlína Pelcová, Vlastimil Kubáň, Isaac K. Kiplagat, Petr Kubáň. Polyelectrolyte multilayers as stationary phases for open tubular ion chromatography. Journal of Chromatography A. 2009; 1216 (51):9022-9026.
Chicago/Turabian StylePavlína Pelcová; Vlastimil Kubáň; Isaac K. Kiplagat; Petr Kubáň. 2009. "Polyelectrolyte multilayers as stationary phases for open tubular ion chromatography." Journal of Chromatography A 1216, no. 51: 9022-9026.
This review provides an update on mercury speciation by CE. It includes a brief discussion on physicochemical properties, toxicity and transformation pathways of mercury species (i.e. methyl-, ethyl-, phenyl- and inorganic mercury) and outlines recent trends in Hg speciation by CE. CE is presented as a complementary technique to chromatographic separation techniques, especially in cases when speed, high efficiency and low sample volumes are required. The development of suitable sample preconcentration/isolation (sample stacking, ion exchange, liquid-liquid-liquid extraction, dual-cloud point extraction) to achieve low LODs for analysis of trace concentrations of mercury species in real samples is emphasized. Hyphenation of CE to element specific detectors (i.e. electrothermal atomic absorption spectrometry, atomic fluorescence spectrometry, inductively coupled plasma-optical emission spectrometry, inductively coupled plasma-mass spectrometry) is discussed as well as a potential of CE in interaction studies that may provide useful information on interaction of various Hg species with selected bio-macromolecules.
Petr Kubánˇ; Pavlína Pelcová; Jana Margetínová; Vlastimil Kubánˇ. Mercury speciation by CE: An update. ELECTROPHORESIS 2009, 30, 92 -99.
AMA StylePetr Kubánˇ, Pavlína Pelcová, Jana Margetínová, Vlastimil Kubánˇ. Mercury speciation by CE: An update. ELECTROPHORESIS. 2009; 30 (1):92-99.
Chicago/Turabian StylePetr Kubánˇ; Pavlína Pelcová; Jana Margetínová; Vlastimil Kubánˇ. 2009. "Mercury speciation by CE: An update." ELECTROPHORESIS 30, no. 1: 92-99.
We describe ion chromatography (IC) on open tubular cation exchange columns with a controllable capacity multilayered stationary phase architecture. The columns of relatively large bore (75 μm id) are fabricated by coating fused‐silica capillaries with multiple layers of poly(butadiene‐maleic acid) (PBMA) copolymer and crosslinking the deposited layers by thermally initiated radical polymerisation. Column capacity increases in a predictable manner with increase in the number of successively coated layers. Gravity flow with a modest head (< 2 m) can provide the desired separations within a reasonable period. We provide a minimalist configuration where no suppression is used, the sample is injected hydrodynamically as in CE, and detection is accomplished by an inexpensive homebuilt contactless conductivity detector or a capacitance to voltage digital converter. A 1 m long 75 μm bore column coated with two layers of PBMA allows gravity‐flow open tubular IC to separate four alkali cations in < 10 min with a 1 mM tartaric acid (TA) eluent. Simultaneous separation of alkali and alkaline earth metal cations can be accomplished in less than 25 min using 1.75 mM pyridinedicarboxylic acid as an eluent. Contactless conductometric detection (C4D) allows LODs down to 150 nmol/L, corresponding to 30 fmol injections. Analysis of real water samples is demonstrated.
Petr Kubáň; Pavlína Pelcová; Vlastimil Kubáň; Lenka Klakurková; Purnendu K. Dasgupta. Gravity-flow open tubular cation chromatography. Journal of Separation Science 2008, 31, 2745 -2753.
AMA StylePetr Kubáň, Pavlína Pelcová, Vlastimil Kubáň, Lenka Klakurková, Purnendu K. Dasgupta. Gravity-flow open tubular cation chromatography. Journal of Separation Science. 2008; 31 (15):2745-2753.
Chicago/Turabian StylePetr Kubáň; Pavlína Pelcová; Vlastimil Kubáň; Lenka Klakurková; Purnendu K. Dasgupta. 2008. "Gravity-flow open tubular cation chromatography." Journal of Separation Science 31, no. 15: 2745-2753.
A high-pressure microwave digestion was applied for microwave-assisted extraction (MAE) of mercury species from sediments and zoobenthos samples. A mixture containing 3 mol L−1 HCl, 50% aqueous methanol and 0.2 mol L−1 citric acid (for masking co-extracted Fe3+) was selected as the most suitable extraction agent. The efficiency of proposed extraction method was better than 95% with R.S.D. below 6%. A preconcentration method utilizing a “homemade” C18 solid phase extraction (SPE) microcolumns was developed to enhance sensitivity of the mercury species determination using on-column complex formation of mercury-2-mercaptophenol complexes. Methanol was chosen for counter-current elution of the retained mercury complexes achieving a preconcentration factor as much as 1000. The preconcentration method was applied for the speciation analysis of mercury in river water samples. The high-performance liquid chromatography-cold vapour atomic fluorescence spectrometric (HPLC/CV-AFS) method was used for the speciation analysis of mercury. The complete separation of four mercury species was achieved by an isocratic elution of aqueous methanol (65%/35%) on a Zorbax SB-C18 column (4.6 mm × 150 mm, 5 μm) using the same complexation reagent (2-mercaptophenol). The limits of detection were 4.3 μg L−1 for methylmercury (MeHg+), 1.4 μg L−1 for ethylmercury (EtHg+), 0.8 μg L−1 for inorganic mercury (Hg2+), 0.8 μg L−1 for phenylmercury (PhHg+).
Jana Margetínová; Pavlína Pelcová; Vlastimil Kubáň. Speciation analysis of mercury in sediments, zoobenthos and river water samples by high-performance liquid chromatography hyphenated to atomic fluorescence spectrometry following preconcentration by solid phase extraction. Analytica Chimica Acta 2008, 615, 115 -123.
AMA StyleJana Margetínová, Pavlína Pelcová, Vlastimil Kubáň. Speciation analysis of mercury in sediments, zoobenthos and river water samples by high-performance liquid chromatography hyphenated to atomic fluorescence spectrometry following preconcentration by solid phase extraction. Analytica Chimica Acta. 2008; 615 (2):115-123.
Chicago/Turabian StyleJana Margetínová; Pavlína Pelcová; Vlastimil Kubáň. 2008. "Speciation analysis of mercury in sediments, zoobenthos and river water samples by high-performance liquid chromatography hyphenated to atomic fluorescence spectrometry following preconcentration by solid phase extraction." Analytica Chimica Acta 615, no. 2: 115-123.