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Sylvie Skalickova
Department of Animal Nutrition and Forage Production, Mendel University in Brno, Zemědělská 1, 61300 Brno, Czech Republic

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Journal article
Published: 05 June 2021 in Animals
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Milk thistle seeds contain a mixture of flavonoids known as silymarin, which consists of silybin, isosilybin, silychristine, and silydianin. Until now, there has been no evidence of monitoring the digestibility of silymarin complex in horses. The aim of the research was to evaluate the digestibility of silymarin complex and the effect of nutrient digestibility in horses. Different daily feed doses (FD) of milk thistle expeller (0 g, 100 g, 200 g, 400 g, 700 g) were administered to five mares kept under the same conditions and at the same feed rations. Digestibility of silymarin complex was monitored by HPLC-UV. Digestible energy (DE), crude protein, crude fat, crude fiber, nitrogen-free extract (NFE), crude ash, calcium (Ca), and phosphorus (P) were determined according ISO/IEC 17025:2017. The biochemical profile of blood plasma (total protein, albumin, alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), bilirubin, total cholesterol, HDL cholesterol, LDL cholesterol, triacyl glyceride (TAG), non-esterified fatty acid (NEFA), creatine kinase (CK), creatinine, urea, glutathione peroxidase (GSH-Px), total antioxidant status (TAS), glucose, calcium, and inorganic phosphate) was investigated. Moreover, the flavonolignans of the silymarin complex in plasma were detected. Statistically significant differences (p ≤ 0.05) were found between daily doses of milk thistle expellers in digestibilities. Our findings showed the digestibility of flavonolignans increased with the daily dose and then stagnated with the dose of milk thistle seed cakes at 700 g/day.

ACS Style

Hana Dockalova; Ladislav Zeman; Daria Baholet; Andrej Batik; Sylvie Skalickova; Pavel Horky. Dose Effect of Milk Thistle (Silybum marianum) Seed Cakes on the Digestibility of Nutrients, Flavonolignans and the Individual Components of the Silymarin Complex in Horses. Animals 2021, 11, 1687 .

AMA Style

Hana Dockalova, Ladislav Zeman, Daria Baholet, Andrej Batik, Sylvie Skalickova, Pavel Horky. Dose Effect of Milk Thistle (Silybum marianum) Seed Cakes on the Digestibility of Nutrients, Flavonolignans and the Individual Components of the Silymarin Complex in Horses. Animals. 2021; 11 (6):1687.

Chicago/Turabian Style

Hana Dockalova; Ladislav Zeman; Daria Baholet; Andrej Batik; Sylvie Skalickova; Pavel Horky. 2021. "Dose Effect of Milk Thistle (Silybum marianum) Seed Cakes on the Digestibility of Nutrients, Flavonolignans and the Individual Components of the Silymarin Complex in Horses." Animals 11, no. 6: 1687.

Journal article
Published: 03 February 2021 in Toxics
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Selenium nanoparticles (SeNPs) are fast becoming a key instrument in several applications such as medicine or nutrition. Questions have been raised about the safety of their use. Male rats were fed for 28 days on a monodiet containing 0.5, 1.5, 3.0 and 5.0 mg Se/kg. Se content in blood and liver, liver panel tests, blood glucose, total antioxidant capacity (TAC), the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) were analysed. Liver and duodenum were subjected to histopathology examination. The weight gain of rats showed no differences between tested groups. Se content in blood was higher in all treated groups compared to the control group. The liver concentration of Se in the treated groups varied in the range from 222 to 238 ng/g. No differences were observed in the activity of AST (aspartate aminotransferase), ALP (alkaline phosphatase) and TAS (total antioxidant status). A significant decrease in ALT activity compared to the control group was observed in the treated groups. GPx activity varied from 80 to 88 U/mL through tested groups. SOD activity in liver was decreased in the SeNP-treated group with 5 mg Se/kg (929 ± 103 U/mL). Histopathological examination showed damage to the liver parenchyma and intestinal epithelium in a dose-dependent manner. This study suggests that short-term SeNP supplementation can be safe and beneficial in Se deficiency or specific treatment.

ACS Style

Lenka Urbankova; Sylvie Skalickova; Magdalena Pribilova; Andrea Ridoskova; Pavlina Pelcova; Jiri Skladanka; Pavel Horky. Effects of Sub-Lethal Doses of Selenium Nanoparticles on the Health Status of Rats. Toxics 2021, 9, 28 .

AMA Style

Lenka Urbankova, Sylvie Skalickova, Magdalena Pribilova, Andrea Ridoskova, Pavlina Pelcova, Jiri Skladanka, Pavel Horky. Effects of Sub-Lethal Doses of Selenium Nanoparticles on the Health Status of Rats. Toxics. 2021; 9 (2):28.

Chicago/Turabian Style

Lenka Urbankova; Sylvie Skalickova; Magdalena Pribilova; Andrea Ridoskova; Pavlina Pelcova; Jiri Skladanka; Pavel Horky. 2021. "Effects of Sub-Lethal Doses of Selenium Nanoparticles on the Health Status of Rats." Toxics 9, no. 2: 28.

Physiology
Published: 23 September 2020 in PLOS ONE
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Mycotoxin management in agriculture is an essential challenge for maintaining the health of both animals and humans. Choosing the right adsorbent is still a question for many breeders and an important criterion for feed manufacturers. New adsorbents are still being sought. Graphene oxide is a promising material in the field of nanotechnology, which excels in its adsorption properties. Presented in vitro study investigates graphene oxide for the binding of mycotoxins from crushed wheat. The results show that graphene oxide has an adsorption capacity for aflatoxin 0.045 mg/g, zearalenone 0.53 mg/g and deoxynivalenol 1.69 mg/g at 37° C. In vitro simulation of crushed wheat digestion showed rapid adsorption during the gastric phase. Of the minerals, Mg, Cu and Zn were the most adsorbed. The applied dose of graphene oxide of 10 mg/g caused only a slight inhibition of the digestive enzymes α-amylase and trypsin compared to pepsin and gastric lipase. In vitro results indicated the suitability of graphene oxide in the adsorption of the aflatoxin, zearalenone and deoxynivalenol.

ACS Style

Pavel Horky; Eva Venusova; Tereza Aulichova; Andrea Ridoskova; Jiri Skladanka; Sylvie Skalickova. Usability of graphene oxide as a mycotoxin binder: In vitro study. PLOS ONE 2020, 15, e0239479 .

AMA Style

Pavel Horky, Eva Venusova, Tereza Aulichova, Andrea Ridoskova, Jiri Skladanka, Sylvie Skalickova. Usability of graphene oxide as a mycotoxin binder: In vitro study. PLOS ONE. 2020; 15 (9):e0239479.

Chicago/Turabian Style

Pavel Horky; Eva Venusova; Tereza Aulichova; Andrea Ridoskova; Jiri Skladanka; Sylvie Skalickova. 2020. "Usability of graphene oxide as a mycotoxin binder: In vitro study." PLOS ONE 15, no. 9: e0239479.

Journal article
Published: 27 May 2020 in Revista Brasileira de Zootecnia
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ACS Style

Ivana Kolackova; Jiri Skladanka; Sylvie Skalickova; Pavel Horky; Natalia Cernei; Zuzana Lackova; Jiri Trinacty; Vojtech Adam. Degradation of biogenic amines and in vitro evaluation of ruminal parameters of the ruminal fluid of Charolais sheep. Revista Brasileira de Zootecnia 2020, 49, 1 .

AMA Style

Ivana Kolackova, Jiri Skladanka, Sylvie Skalickova, Pavel Horky, Natalia Cernei, Zuzana Lackova, Jiri Trinacty, Vojtech Adam. Degradation of biogenic amines and in vitro evaluation of ruminal parameters of the ruminal fluid of Charolais sheep. Revista Brasileira de Zootecnia. 2020; 49 ():1.

Chicago/Turabian Style

Ivana Kolackova; Jiri Skladanka; Sylvie Skalickova; Pavel Horky; Natalia Cernei; Zuzana Lackova; Jiri Trinacty; Vojtech Adam. 2020. "Degradation of biogenic amines and in vitro evaluation of ruminal parameters of the ruminal fluid of Charolais sheep." Revista Brasileira de Zootecnia 49, no. : 1.

Journal article
Published: 05 March 2020 in International Journal of Molecular Sciences
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It is generally believed that antibacterial essential oils have the potential to become one of the alternatives in preventing diarrheal diseases of monogastric animals. The disadvantage is their low efficiency per oral due to easy degradation during digestion in the stomach. This study compares the efficacy of chitosan, alginate-chitosan, guar gum-chitosan, xanthan gum-chitosan and pectin-chitosan nanocapsules to the synthesis of pH-responsive biopolymeric nanocapsule for Thymus vulgaris, Rosmarinus officinalis and Syzygium aromaticum essential oils. Using spectrophotometric approach and gas chromatography, release kinetics were determined in pH 3, 5.6 and 7.4. The growth rates of S. aureus and E. coli, as well as minimal inhibition concentration of essential oils were studied. The average encapsulation efficiency was 60%, and the loading efficiency was 70%. The size of the nanocapsules ranged from 100 nm to 500 nm. Results showed that chitosan-guar gum and chitosan-pectin nanocapsules released 30% of essential oils (EOs) at pH 3 and 80% at pH 7.4 during 3 h. Similar release kinetics were confirmed for thymol, eugenol and α-pinene. Minimal inhibition concentrations of Thymus vulgaris and Syzygium aromaticum essential oils ranged from 0.025 to 0.5%. Findings of this study suggest that the suitable pH-responsive nanocapsule for release, low toxicity and antibacterial activity is based on chitosan-guar gum structure.

ACS Style

Sylvie Skalickova; Tereza Aulichova; Eva Venusova; Jiri Skladanka; Pavel Horky. Development of pH-Responsive Biopolymeric Nanocapsule for Antibacterial Essential Oils. International Journal of Molecular Sciences 2020, 21, 1799 .

AMA Style

Sylvie Skalickova, Tereza Aulichova, Eva Venusova, Jiri Skladanka, Pavel Horky. Development of pH-Responsive Biopolymeric Nanocapsule for Antibacterial Essential Oils. International Journal of Molecular Sciences. 2020; 21 (5):1799.

Chicago/Turabian Style

Sylvie Skalickova; Tereza Aulichova; Eva Venusova; Jiri Skladanka; Pavel Horky. 2020. "Development of pH-Responsive Biopolymeric Nanocapsule for Antibacterial Essential Oils." International Journal of Molecular Sciences 21, no. 5: 1799.

Review
Published: 13 June 2019 in Animals
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Essential oils (EOs) are now a hot topic in finding modern substitutes for antibiotics. Many studies have shown positive results and confirmed their high antibacterial activity both in vitro and in vivo. Deservedly, there is an attempt to use EOs as a substitute for antibiotics, which are currently limited by legislation in animal breeding. Given the potential of EOs, studies on their fate in the body need to be summarized. The content of EO's active substances varies depending on growing conditions and consequently on processing and storage. Their content also changes dynamically during the passage through the gastrointestinal tract and their effective concentration can be noticeably diluted at their place of action (small intestine and colon). Based on the solubility of the individual EO's active substances, they are eliminated from the body at different rates. Despite a strong antimicrobial effect, some oils can be toxic to the body and cause damage to the liver, kidneys, or gastrointestinal tissues. Reproductive toxicity has been reported for Origanum vulgare and Mentha arvensis. Several publications also address the effect on the genome. It has been observed that EOs can show both genoprotective effects (Syzygium aromaticum) and genotoxicity, as is the case of Cinnamomum camphor. This review shows that although oils are mainly studied as promising antimicrobials, it is also important to assess animal safety.

ACS Style

Pavel Horky; Sylvie Skalickova; Kristyna Smerkova; Jiri Skladanka. Essential Oils as a Feed Additives: Pharmacokinetics and Potential Toxicity in Monogastric Animals. Animals 2019, 9, 352 .

AMA Style

Pavel Horky, Sylvie Skalickova, Kristyna Smerkova, Jiri Skladanka. Essential Oils as a Feed Additives: Pharmacokinetics and Potential Toxicity in Monogastric Animals. Animals. 2019; 9 (6):352.

Chicago/Turabian Style

Pavel Horky; Sylvie Skalickova; Kristyna Smerkova; Jiri Skladanka. 2019. "Essential Oils as a Feed Additives: Pharmacokinetics and Potential Toxicity in Monogastric Animals." Animals 9, no. 6: 352.

Journal article
Published: 12 February 2019 in Journal of Animal Science and Biotechnology
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Development of new nanomaterials that inhibit or kill bacteria is an important and timely research topic. For example, financial losses due to infectious diseases, such as diarrhea, are a major concern in livestock productions around the world. Antimicrobial nanoparticles (NPs) represent a promising alternative to antibiotics and may lower antibiotic use and consequently spread of antibiotic resistance traits among bacteria, including pathogens. Four formulations of zinc nanoparticles (ZnA, ZnB, ZnC, and ZnD) based on phosphates with spherical (ZnA, ZnB) or irregular (ZnC, ZnD) morphology were prepared. The highest in vitro inhibitory effect of our NPs was observed against Staphylococcus aureus (inhibitory concentration values, IC50, ranged from 0.5 to 1.6 mmol/L), followed by Escherichia coli (IC50 0.8–1.5 mmol/L). In contrast, methicillin resistant S. aureus (IC50 1.2–4.7 mmol/L) was least affected and this was similar to inhibitory patterns of commercial ZnO-based NPs and ZnO. After the successful in vitro testing, the in vivo study with rats based on dietary supplementation with zinc NPs was conducted. Four groups of rats were treated by 2,000 mg Zn/kg diet of ZnA, ZnB, ZnC, and ZnD, for comparison two groups were supplemented by 2,000 mg Zn/kg diet of ZnO-N and ZnO, and one group (control) was fed only by basal diet. The significantly higher (P < 0.05) Zn level in liver and kidney of all treated groups was found, nevertheless Zn NPs did not greatly influence antioxidant status of rats. However, the total aerobic and coliform bacterial population in rat feces significantly decreased (P < 0.05) in all zinc groups after 30 d of the treatment. Furthermore, when compared to the ZnO group, ZnA and ZnC nanoparticles reduced coliforms significantly more (P < 0.05). Our results demonstrate that phosphate-based zinc nanoparticles have the potential to act as antibiotic agents.

ACS Style

Pavel Horky; Sylvie Skalickova; Lenka Urbankova; Daria Baholet; Silvia Kociova; Zuzana Bytešníková; Eliska Kabourkova; Zuzana Lackova; Natalia Cernei; Milica Gagic; Vedran Milosavljevic; Vendula Smolikova; Eva Vaclavkova; Pavel Nevrkla; Pavel Knot; Olga Krystofova; David Hynek; Pavel Kopel; Jiri Skladanka; Vojtech Adam; Kristyna Smerkova. Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure. Journal of Animal Science and Biotechnology 2019, 10, 1 -12.

AMA Style

Pavel Horky, Sylvie Skalickova, Lenka Urbankova, Daria Baholet, Silvia Kociova, Zuzana Bytešníková, Eliska Kabourkova, Zuzana Lackova, Natalia Cernei, Milica Gagic, Vedran Milosavljevic, Vendula Smolikova, Eva Vaclavkova, Pavel Nevrkla, Pavel Knot, Olga Krystofova, David Hynek, Pavel Kopel, Jiri Skladanka, Vojtech Adam, Kristyna Smerkova. Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure. Journal of Animal Science and Biotechnology. 2019; 10 (1):1-12.

Chicago/Turabian Style

Pavel Horky; Sylvie Skalickova; Lenka Urbankova; Daria Baholet; Silvia Kociova; Zuzana Bytešníková; Eliska Kabourkova; Zuzana Lackova; Natalia Cernei; Milica Gagic; Vedran Milosavljevic; Vendula Smolikova; Eva Vaclavkova; Pavel Nevrkla; Pavel Knot; Olga Krystofova; David Hynek; Pavel Kopel; Jiri Skladanka; Vojtech Adam; Kristyna Smerkova. 2019. "Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure." Journal of Animal Science and Biotechnology 10, no. 1: 1-12.

Journal article
Published: 15 October 2018 in KVASNY PRUMYSL
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ACS Style

Pavel Horky; Sylvie Skalickova; Iva Caslavova; Amanda J. Deering; Pavel Nevrkla; Petr Slama; Vaclav Trojan; Jiri Skladanka. Effect of fungicidal treatment and storage condition on content of selected mycotoxins in barley. KVASNY PRUMYSL 2018, 64, 212 -216.

AMA Style

Pavel Horky, Sylvie Skalickova, Iva Caslavova, Amanda J. Deering, Pavel Nevrkla, Petr Slama, Vaclav Trojan, Jiri Skladanka. Effect of fungicidal treatment and storage condition on content of selected mycotoxins in barley. KVASNY PRUMYSL. 2018; 64 (5):212-216.

Chicago/Turabian Style

Pavel Horky; Sylvie Skalickova; Iva Caslavova; Amanda J. Deering; Pavel Nevrkla; Petr Slama; Vaclav Trojan; Jiri Skladanka. 2018. "Effect of fungicidal treatment and storage condition on content of selected mycotoxins in barley." KVASNY PRUMYSL 64, no. 5: 212-216.

Review
Published: 14 September 2018 in Nanomaterials
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Mycotoxins are toxic secondary metabolites produced by certain filamentous fungi. The occurrence of mycotoxins in food and feed causes negative health impacts on both humans and animals. Clay binders, yeast cell walls, or antioxidant additives are the most widely used products for mycotoxin elimination to reduce their impact. Although conventional methods are constantly improving, current research trends are looking for innovative solutions. Nanotechnology approaches seem to be a promising, effective, and low-cost way to minimize the health effects of mycotoxins. This review aims to shed light on the critical knowledge gap in mycotoxin elimination by nanotechnology. There are three main strategies: mold inhibition, mycotoxin adsorption, and reducing the toxic effect via nanoparticles. One of the most promising methods is the use of carbon-based nanomaterials. Graphene has been shown to have a huge surface and high binding capacity for mycotoxins. Attention has also been drawn to polymeric nanoparticles; they could substitute adsorbents or enclose any substance, which would improve the health status of the organism. In light of these findings, this review gives new insights into possible future research that might overcome challenges associated with nanotechnology utilization for mycotoxin elimination from agricultural products.

ACS Style

Pavel Horky; Sylvie Skalickova; Daria Baholet; Jiri Skladanka. Nanoparticles as a Solution for Eliminating the Risk of Mycotoxins. Nanomaterials 2018, 8, 727 .

AMA Style

Pavel Horky, Sylvie Skalickova, Daria Baholet, Jiri Skladanka. Nanoparticles as a Solution for Eliminating the Risk of Mycotoxins. Nanomaterials. 2018; 8 (9):727.

Chicago/Turabian Style

Pavel Horky; Sylvie Skalickova; Daria Baholet; Jiri Skladanka. 2018. "Nanoparticles as a Solution for Eliminating the Risk of Mycotoxins." Nanomaterials 8, no. 9: 727.

Review
Published: 01 April 2018 in International Journal of Nanomedicine
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Nano-selenium and its nanomedicine applications: a critical review Bozena Hosnedlova,1 Marta Kepinska,2 Sylvie Skalickova,3 Carlos Fernandez,4 Branislav Ruttkay-Nedecky,3 Qiuming Peng,5 Mojmir Baron,1 Magdalena Melcova,6 Radka Opatrilova,3 Jarmila Zidkova,6 Geir Bjørklund,7 Jiri Sochor,1 Rene Kizek2,3 1Department of Viticulture and Enology, Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic; 2Department of Biomedical and Environmental Analyses, Faculty of Pharmacy, Wroclaw Medical University, Wroclaw, Poland; 3Central Laboratory, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic; 4School of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen, UK; 5State Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao, People’s Republic of China; 6Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Czech Republic; 7Council for Nutritional and Environmental Medicine, Rana, Norway Traditional supplements of selenium generally have a low degree of absorption and increased toxicity. Therefore, it is imperative to develop innovative systems as transporters of selenium compounds, which would raise the bioavailability of this element and allow its controlled release in the organism. Nanoscale selenium has attracted a great interest as a food additive especially in individuals with selenium deficiency, but also as a therapeutic agent without significant side effects in medicine. This review is focused on the incorporation of nanotechnological applications, in particular exploring the possibilities of a more effective way of administration, especially in selenium-deficient organisms. In addition, this review summarizes the survey of knowledge on selenium nanoparticles, their biological effects in the organism, advantages, absorption mechanisms, and nanotechnological applications for peroral administration. Keywords: nanoparticles, biomedicine, drug delivery, oxidative stress, anticancer effect, antimicrobial acitivity, protective effect

ACS Style

Bozena Hosnedlova; Marta Kepinska; Sylvie Skalickova; Carlos Fernandez; Branislav Ruttkay-Nedecky; Qiuming Peng; Mojmir Baron; Magdalena Melcova; Radka Opatrilova; Jarmila Zidkova; Geir Bjørklund; Jiri Sochor; Rene Kizek. Nano-selenium and its nanomedicine applications: a critical review. International Journal of Nanomedicine 2018, ume 13, 2107 -2128.

AMA Style

Bozena Hosnedlova, Marta Kepinska, Sylvie Skalickova, Carlos Fernandez, Branislav Ruttkay-Nedecky, Qiuming Peng, Mojmir Baron, Magdalena Melcova, Radka Opatrilova, Jarmila Zidkova, Geir Bjørklund, Jiri Sochor, Rene Kizek. Nano-selenium and its nanomedicine applications: a critical review. International Journal of Nanomedicine. 2018; ume 13 ():2107-2128.

Chicago/Turabian Style

Bozena Hosnedlova; Marta Kepinska; Sylvie Skalickova; Carlos Fernandez; Branislav Ruttkay-Nedecky; Qiuming Peng; Mojmir Baron; Magdalena Melcova; Radka Opatrilova; Jarmila Zidkova; Geir Bjørklund; Jiri Sochor; Rene Kizek. 2018. "Nano-selenium and its nanomedicine applications: a critical review." International Journal of Nanomedicine ume 13, no. : 2107-2128.

Review
Published: 15 December 2017 in KVASNY PRUMYSL
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ACS Style

Sylvie Skalickova; Mojmir Baron; Jiri Sochor. Nanoparticles Biosynthesized by Yeast: A Review of their application. KVASNY PRUMYSL 2017, 63, 290 -292.

AMA Style

Sylvie Skalickova, Mojmir Baron, Jiri Sochor. Nanoparticles Biosynthesized by Yeast: A Review of their application. KVASNY PRUMYSL. 2017; 63 (6):290-292.

Chicago/Turabian Style

Sylvie Skalickova; Mojmir Baron; Jiri Sochor. 2017. "Nanoparticles Biosynthesized by Yeast: A Review of their application." KVASNY PRUMYSL 63, no. 6: 290-292.

Journal article
Published: 08 December 2017 in Nanomaterials
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This work investigated the preparation of chitosan nanoparticles used as carriers for doxorubicin for targeted cancer delivery. Prepared nanocarriers were stabilized and functionalized via zinc ions incorporated into the chitosan nanoparticle backbone. We took the advantage of high expression of sarcosine in the prostate cancer cells. The prostate cancer targeting was mediated by the AntiSar antibodies decorated surface of the nanocage. Formation of the chitosan nanoparticles was determined using a ninhydrin assay and differential pulse voltammetry. Obtained results showed the strong effect of tripolyphosphine on the nanoparticle formation. The zinc ions affected strong chitosan backbone coiling both in inner and outer chitosan nanoparticle structure. Zinc electrochemical signal depended on the level of the complex formation and the potential shift from −960 to −950 mV. Formed complex is suitable for doxorubicin delivery. It was observed the 20% entrapment efficiency of doxorubicin and strong dependence of drug release after 120 min in the blood environment. The functionality of the designed nanotransporter was proven. The purposed determination showed linear dependence in the concentration range of Anti-sarcosine IgG labeled gold nanoparticles from 0 to 1000 µg/mL and the regression equation was found to be y = 3.8x − 66.7 and R2 = 0.99. Performed ELISA confirmed the ability of Anti-sarcosine IgG labeled chitosan nanoparticles with loaded doxorubicin to bind to the sarcosine molecule. Observed hemolytic activity of the nanotransporter was 40%. Inhibition activity of our proposed nanotransporter was evaluated to be 0% on the experimental model of S. cerevisiae. Anti-sarcosine IgG labeled chitosan nanoparticles, with loaded doxorubicin stabilized by Zn ions, are a perspective type of nanocarrier for targeted drug therapy managed by specific interaction with sarcosine and metallothionein for prostate cancer.

ACS Style

Sylvie Skalickova; Martin Loffelmann; Michael Gargulak; Marta Kepinska; Michaela Docekalova; Dagmar Uhlirova; Martina Stankova; Carlos Fernandez; Halina Milnerowicz; Branislav Ruttkay-Nedecky; Rene Kizek. Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery. Nanomaterials 2017, 7, 435 .

AMA Style

Sylvie Skalickova, Martin Loffelmann, Michael Gargulak, Marta Kepinska, Michaela Docekalova, Dagmar Uhlirova, Martina Stankova, Carlos Fernandez, Halina Milnerowicz, Branislav Ruttkay-Nedecky, Rene Kizek. Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery. Nanomaterials. 2017; 7 (12):435.

Chicago/Turabian Style

Sylvie Skalickova; Martin Loffelmann; Michael Gargulak; Marta Kepinska; Michaela Docekalova; Dagmar Uhlirova; Martina Stankova; Carlos Fernandez; Halina Milnerowicz; Branislav Ruttkay-Nedecky; Rene Kizek. 2017. "Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery." Nanomaterials 7, no. 12: 435.

Review
Published: 21 October 2017 in International Journal of Molecular Sciences
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Selenium is an essential trace element important for many physiological processes, especially for the functions of immune and reproductive systems, metabolism of thyroid hormones, as well as antioxidant defense. Selenium deficiency is usually manifested by an increased incidence of retention of placenta, metritis, mastitis, aborts, lowering fertility and increased susceptibility to infections. In calves, lambs and kids, the selenium deficiency demonstrates by WMD (white muscle disease), in foals and donkey foals, it is associated with incidence of WMD and yellow fat disease, and in pigs it causes VESD (vitamin E/selenium deficiency) syndrome. The prevention of these health disorders can be achieved by an adequate selenium supplementation to the diet. The review summarizes the survey of knowledge on selenium, its biological significance in the organism, the impact of its deficiency in mammalian livestock (comparison of ruminants vs. non-ruminants, herbivore vs. omnivore) and possibilities of its peroral administration. The databases employed were as follows: Web of Science, PubMed, MEDLINE and Google Scholar.

ACS Style

Bozena Hosnedlova; Marta Kepinska; Sylvie Skalickova; Carlos Fernandez; Branislav Ruttkay-Nedecky; Thembinkosi Donald Malevu; Jiri Sochor; Mojmir Baron; Magdalena Melcova; Jarmila Zidkova; Rene Kizek. A Summary of New Findings on the Biological Effects of Selenium in Selected Animal Species—A Critical Review. International Journal of Molecular Sciences 2017, 18, 2209 .

AMA Style

Bozena Hosnedlova, Marta Kepinska, Sylvie Skalickova, Carlos Fernandez, Branislav Ruttkay-Nedecky, Thembinkosi Donald Malevu, Jiri Sochor, Mojmir Baron, Magdalena Melcova, Jarmila Zidkova, Rene Kizek. A Summary of New Findings on the Biological Effects of Selenium in Selected Animal Species—A Critical Review. International Journal of Molecular Sciences. 2017; 18 (10):2209.

Chicago/Turabian Style

Bozena Hosnedlova; Marta Kepinska; Sylvie Skalickova; Carlos Fernandez; Branislav Ruttkay-Nedecky; Thembinkosi Donald Malevu; Jiri Sochor; Mojmir Baron; Magdalena Melcova; Jarmila Zidkova; Rene Kizek. 2017. "A Summary of New Findings on the Biological Effects of Selenium in Selected Animal Species—A Critical Review." International Journal of Molecular Sciences 18, no. 10: 2209.

Journal article
Published: 05 May 2017 in Klinicka onkologie
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ACS Style

Sylvie Skalickova; M Löfelmann; M Gargulák; M Dočekalová; B Ruttkay-Nedecký; P Suchý; M Kepinská; R Kizek. [Fullerene Doxorubicin Nanotransporter for Target Interaction with mutated gene BRCA2]. Klinicka onkologie 2017, 30, 177 -179.

AMA Style

Sylvie Skalickova, M Löfelmann, M Gargulák, M Dočekalová, B Ruttkay-Nedecký, P Suchý, M Kepinská, R Kizek. [Fullerene Doxorubicin Nanotransporter for Target Interaction with mutated gene BRCA2]. Klinicka onkologie. 2017; 30 ():177-179.

Chicago/Turabian Style

Sylvie Skalickova; M Löfelmann; M Gargulák; M Dočekalová; B Ruttkay-Nedecký; P Suchý; M Kepinská; R Kizek. 2017. "[Fullerene Doxorubicin Nanotransporter for Target Interaction with mutated gene BRCA2]." Klinicka onkologie 30, no. : 177-179.

Journal article
Published: 05 May 2017 in Klinicka onkologie
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ACS Style

Sylvie Skalickova; M Gargulák; M Löffelmann; B Ruttkay-Nedecký; M Kepinská; T Parák; R Kizek. [Zinc-modified Nanotransporter for Target Drug Therapy of Breast Cancer]. Klinicka onkologie 2017, 30, 174 -176.

AMA Style

Sylvie Skalickova, M Gargulák, M Löffelmann, B Ruttkay-Nedecký, M Kepinská, T Parák, R Kizek. [Zinc-modified Nanotransporter for Target Drug Therapy of Breast Cancer]. Klinicka onkologie. 2017; 30 ():174-176.

Chicago/Turabian Style

Sylvie Skalickova; M Gargulák; M Löffelmann; B Ruttkay-Nedecký; M Kepinská; T Parák; R Kizek. 2017. "[Zinc-modified Nanotransporter for Target Drug Therapy of Breast Cancer]." Klinicka onkologie 30, no. : 174-176.

Journal article
Published: 01 February 2017 in Journal of Pharmaceutical and Biomedical Analysis
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Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae are the most representative bacteria causing infectious diseases. Due to the increased application of antibiotics, the bacterial resistance is growing causing severe complications. Therefore, a sensitive determination of these pathogens is crucial for effective treatment. The aim of this study was to design an effective method for multiplex detection of Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae taking advantage from properties of magnetic particles as well as fluorescent nanoparticles (quantum dots). The method was able to detect as low concentrations of bacteria as 10 CFU/mL using the bacteria-specific genes (fnbA, mecA and wcaG).

ACS Style

Kristyna Cihalova; Dagmar Hegerová; Ana María Jiménez Jiménez; Vedran Milosavljević; Jiri Kudr; Sylvie Skalickova; David Hynek; Pavel Kopel; Marketa Vaculovicova; Vojtech Adam. Antibody-free detection of infectious bacteria using quantum dots-based barcode assay. Journal of Pharmaceutical and Biomedical Analysis 2017, 134, 325 -332.

AMA Style

Kristyna Cihalova, Dagmar Hegerová, Ana María Jiménez Jiménez, Vedran Milosavljević, Jiri Kudr, Sylvie Skalickova, David Hynek, Pavel Kopel, Marketa Vaculovicova, Vojtech Adam. Antibody-free detection of infectious bacteria using quantum dots-based barcode assay. Journal of Pharmaceutical and Biomedical Analysis. 2017; 134 ():325-332.

Chicago/Turabian Style

Kristyna Cihalova; Dagmar Hegerová; Ana María Jiménez Jiménez; Vedran Milosavljević; Jiri Kudr; Sylvie Skalickova; David Hynek; Pavel Kopel; Marketa Vaculovicova; Vojtech Adam. 2017. "Antibody-free detection of infectious bacteria using quantum dots-based barcode assay." Journal of Pharmaceutical and Biomedical Analysis 134, no. : 325-332.

Review
Published: 18 May 2016 in Nutrition
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Selenium is an essential trace element in the diet, required for maintenance of health and growth; however, its toxicity could cause serious damage depending on dose and chemical form. Selenium nanoparticles (SeNPs) represent what we believe to be a novel prospect for nutritional supplementation because of their lower toxicity and ability to gradually release selenium after ingestion. In this review, we discuss various forms and types of SeNPs, as well as the way they are synthesized. We also discuss absorption and bioavailability of nanoparticles within the organism. SeNPs demonstrate anticancer and antimicrobial properties that may contribute to human health, not only as dietary supplements, but also as therapeutic agents.

ACS Style

Sylvie Skalickova; Vedran Milosavljevic; Kristyna Cihalova; Pavel Horky; Lukas Richtera; Vojtech Adam. Selenium nanoparticles as a nutritional supplement. Nutrition 2016, 33, 83 -90.

AMA Style

Sylvie Skalickova, Vedran Milosavljevic, Kristyna Cihalova, Pavel Horky, Lukas Richtera, Vojtech Adam. Selenium nanoparticles as a nutritional supplement. Nutrition. 2016; 33 ():83-90.

Chicago/Turabian Style

Sylvie Skalickova; Vedran Milosavljevic; Kristyna Cihalova; Pavel Horky; Lukas Richtera; Vojtech Adam. 2016. "Selenium nanoparticles as a nutritional supplement." Nutrition 33, no. : 83-90.

Journal article
Published: 25 February 2016 in Sensors
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Liposome-based drug delivery systems hold great potential for cancer therapy. The aim of this study was to design a nanodevice for targeted anchoring of liposomes (with and without cholesterol) with encapsulated anticancer drugs and antisense N-myc gene oligonucleotide attached to its surface. To meet this main aim, liposomes with encapsulated doxorubicin, ellipticine and etoposide were prepared. They were further characterized by measuring their fluorescence intensity, whereas the encapsulation efficiency was estimated to be 16%. The hybridization process of individual oligonucleotides forming the nanoconstruct was investigated spectrophotometrically and electrochemically. The concentrations of ellipticine, doxorubicin and etoposide attached to the nanoconstruct in gold nanoparticle-modified liposomes were found to be 14, 5 and 2 µg·mL−1, respectively. The study succeeded in demonstrating that liposomes are suitable for the transport of anticancer drugs and the antisense oligonucleotide, which can block the expression of the N-myc gene.

ACS Style

Sylvie Skalickova; Lukas Nejdl; Jiri Kudr; Branislav Ruttkay-Nedecky; Ana Maria Jimenez Jimenez; Pavel Kopel; Monika Kremplova; Michal Masarik; Marie Stiborova; Tomas Eckschlager; Vojtech Adam; Rene Kizek. Fluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide). Sensors 2016, 16, 290 .

AMA Style

Sylvie Skalickova, Lukas Nejdl, Jiri Kudr, Branislav Ruttkay-Nedecky, Ana Maria Jimenez Jimenez, Pavel Kopel, Monika Kremplova, Michal Masarik, Marie Stiborova, Tomas Eckschlager, Vojtech Adam, Rene Kizek. Fluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide). Sensors. 2016; 16 (3):290.

Chicago/Turabian Style

Sylvie Skalickova; Lukas Nejdl; Jiri Kudr; Branislav Ruttkay-Nedecky; Ana Maria Jimenez Jimenez; Pavel Kopel; Monika Kremplova; Michal Masarik; Marie Stiborova; Tomas Eckschlager; Vojtech Adam; Rene Kizek. 2016. "Fluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide)." Sensors 16, no. 3: 290.

Review
Published: 20 October 2015 in Viruses
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The threat of a worldwide influenza pandemic has greatly increased over the past decade with the emergence of highly virulent avian influenza strains. The increased frequency of drug-resistant influenza strains against currently available antiviral drugs requires urgent development of new strategies for antiviral therapy, too. The research in the field of therapeutic peptides began to develop extensively in the second half of the 20th century. Since then, the mechanisms of action for several peptides and their antiviral prospect received large attention due to the global threat posed by viruses. Here, we discussed the therapeutic properties of peptides used in influenza treatment. Peptides with antiviral activity against influenza can be divided into three main groups. First, entry blocker peptides such as a Flupep that interact with influenza hemagglutinin, block its binding to host cells and prevent viral fusion. Second, several peptides display virucidal activity, disrupting viral envelopes, e.g., Melittin. Finally, a third set of peptides interacts with the viral polymerase complex and act as viral replication inhibitors such as PB1 derived peptides. Here, we present a review of the current literature describing the antiviral activity, mechanism and future therapeutic potential of these influenza antiviral peptides.

ACS Style

Sylvie Skalickova; Zbynek Heger; Ludmila Krejcova; Vladimir Pekarik; Karel Bastl; Jozef Janda; Frantisek Kostolansky; Eva Vareckova; Ondrej Zitka; Vojtech Adam; Rene Kizek. Perspective of Use of Antiviral Peptides against Influenza Virus. Viruses 2015, 7, 5428 -5442.

AMA Style

Sylvie Skalickova, Zbynek Heger, Ludmila Krejcova, Vladimir Pekarik, Karel Bastl, Jozef Janda, Frantisek Kostolansky, Eva Vareckova, Ondrej Zitka, Vojtech Adam, Rene Kizek. Perspective of Use of Antiviral Peptides against Influenza Virus. Viruses. 2015; 7 (10):5428-5442.

Chicago/Turabian Style

Sylvie Skalickova; Zbynek Heger; Ludmila Krejcova; Vladimir Pekarik; Karel Bastl; Jozef Janda; Frantisek Kostolansky; Eva Vareckova; Ondrej Zitka; Vojtech Adam; Rene Kizek. 2015. "Perspective of Use of Antiviral Peptides against Influenza Virus." Viruses 7, no. 10: 5428-5442.

Review
Published: 05 June 2015 in ELECTROPHORESIS
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Nanofluidics is becoming an extensively developing technique in the field of bioanalytical chemistry. Nanoscale hole embed in an insulating membrane is employed in a vast variety of sensing platforms and applications. Although, biological nanopores have several attractive characteristics, in this paper, we focused on the solid‐state nanopores due to their advantages as high stability, possibility of diameter control, and ease of surface functionalizing. A detection method, based on the translocation of analyzed molecules through nanochannels under applied voltage bias and resistive pulse sensing, is well established. Nevertheless, it seems that the new detection methods like measuring of transverse electron tunneling using nanogap electrodes or optical detection can offer significant additional advantages. The aim of this review is not to cite all related articles, but highlight the steps, which in our opinion, meant important progresses in solid‐state nanopore analysis.

ACS Style

Jiri Kudr; Sylvie Skalickova; Lukas Nejdl; Amitava Moulick; Branislav Ruttkay-Nedecky; Vojtech Adam; Rene Kizek. Fabrication of solid-state nanopores and its perspectives. ELECTROPHORESIS 2015, 36, 2367 -2379.

AMA Style

Jiri Kudr, Sylvie Skalickova, Lukas Nejdl, Amitava Moulick, Branislav Ruttkay-Nedecky, Vojtech Adam, Rene Kizek. Fabrication of solid-state nanopores and its perspectives. ELECTROPHORESIS. 2015; 36 (19):2367-2379.

Chicago/Turabian Style

Jiri Kudr; Sylvie Skalickova; Lukas Nejdl; Amitava Moulick; Branislav Ruttkay-Nedecky; Vojtech Adam; Rene Kizek. 2015. "Fabrication of solid-state nanopores and its perspectives." ELECTROPHORESIS 36, no. 19: 2367-2379.