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Anhydroexfoliamycin, a secondary metabolite from Streptomyces, has shown antioxidant properties in primary cortical neurons reducing neurodegenerative hallmarks diseases, both in vitro and in vivo models. Activated microglia, in the central nervous system, plays a crucial role in neuroinflammation and is associated with neurodegeneration. Therefore, the aim of the present study was to determine the anti-inflammatory and antioxidant potential of the anhydroexfoliamycin over microglia BV2 cells. Neuroinflammation was simulated by incubation of microglia cells in the presence of lipopolysaccharide to activate proinflammatory transduction pathways. Moreover, a coculture of neuron SH-SY5Y and microglia BV2 cells was used to evaluate the neuroprotective properties of the Streptomyces metabolite. When microglia cells were preincubated with anhydroexfoliamycin, proinflammatory pathways, such as the translocation of the nuclear factor κB, the phosphorylation of c-Jun N-terminal kinase, and the inducible nitric oxide synthase expression, were inhibited. In addition, intracellular reactive oxygen species generation and the liberation of nitric oxide, interleukin 6, and tumor necrosis factor α were also decreased. Besides, the Streptomyces-derived compound showed antioxidant properties promoting the translocation of the factor erythroid 2-related factor 2 and protecting the SH-SY5Y cells from the neurotoxic mediators released by activated microglia. The effects of this compound were at the same level as the immunosuppressive drug cyclosporine A. Therefore, these results indicate that anhydroexfoliamycin is a promising tool to control microglia-driven inflammation with therapeutic potential in neuroinflammation.
Sandra Gegunde; Amparo Alfonso; Rebeca Alvariño; Nadia Pérez-Fuentes; Luis M. Botana. Anhydroexfoliamycin, a Streptomyces Secondary Metabolite, Mitigates Microglia-Driven Inflammation. ACS Chemical Neuroscience 2021, 12, 2336 -2346.
AMA StyleSandra Gegunde, Amparo Alfonso, Rebeca Alvariño, Nadia Pérez-Fuentes, Luis M. Botana. Anhydroexfoliamycin, a Streptomyces Secondary Metabolite, Mitigates Microglia-Driven Inflammation. ACS Chemical Neuroscience. 2021; 12 (13):2336-2346.
Chicago/Turabian StyleSandra Gegunde; Amparo Alfonso; Rebeca Alvariño; Nadia Pérez-Fuentes; Luis M. Botana. 2021. "Anhydroexfoliamycin, a Streptomyces Secondary Metabolite, Mitigates Microglia-Driven Inflammation." ACS Chemical Neuroscience 12, no. 13: 2336-2346.
Co-occurrence of emerging and regulated mycotoxins in contaminated samples has been widely documented, but studies about their combined toxicity are scarce. In this report, the regulated mycotoxins deoxynivalenol, fumonisin B1 and zearalenone, and the emerging ones enniatin A, enniatin B and beauvericin were tested in SH-SY5Y human neuroblastoma cells. Their individual and binary combined effects on cell viability and mitochondrial function were evaluated. The results with individual mycotoxins revealed that deoxynivalenol and emerging mycotoxins were the most damaging to neuronal cells, presenting IC50 values between 0.35 and 2.4 μM. Interestingly, non-regulated mycotoxins triggered apoptosis by affecting to mitochondrial membrane potential. However, when regulated and non-regulated mycotoxins were binary mixed, antagonistic effects were found in all cases. Finally, cow feed and milk extracts were analysed by UHPLC-MS/MS, detecting the presence of several mycotoxins included in this study. These extracts were tested in neuroblastoma cells, and damaging effects on cell viability were found. Although binary combinations of mycotoxins produced antagonistic effects, their mixture in natural matrixes induces greater effects than expected. Therefore, it would be interesting to explore the matrix influence on mycotoxin toxicity, and to continue studying the neurotoxic mechanism of action of emerging mycotoxins, as they could be a health hazard.
Nadia Pérez-Fuentes; Rebeca Alvariño; Amparo Alfonso; Jesús González-Jartín; Sandra Gegunde; Mercedes R. Vieytes; Luis M. Botana. Single and combined effects of regulated and emerging mycotoxins on viability and mitochondrial function of SH-SY5Y cells. Food and Chemical Toxicology 2021, 154, 112308 .
AMA StyleNadia Pérez-Fuentes, Rebeca Alvariño, Amparo Alfonso, Jesús González-Jartín, Sandra Gegunde, Mercedes R. Vieytes, Luis M. Botana. Single and combined effects of regulated and emerging mycotoxins on viability and mitochondrial function of SH-SY5Y cells. Food and Chemical Toxicology. 2021; 154 ():112308.
Chicago/Turabian StyleNadia Pérez-Fuentes; Rebeca Alvariño; Amparo Alfonso; Jesús González-Jartín; Sandra Gegunde; Mercedes R. Vieytes; Luis M. Botana. 2021. "Single and combined effects of regulated and emerging mycotoxins on viability and mitochondrial function of SH-SY5Y cells." Food and Chemical Toxicology 154, no. : 112308.
Cyclophilins (Cyps) are a group of peptidyl-prolyl cis/trans isomerases that play crucial roles in regulatory mechanisms of cellular physiology and pathology in several inflammatory conditions. Their receptor, CD147, also participates in the development and progression of the inflammatory response. Nevertheless, the main function of Cyps and their receptor are yet to be deciphered. The release of CypA and the expression of the CD147 receptor in activated T lymphocytes were already described, however, no data are available about other Cyps in these cells. Therefore, in the present work intra and extracellular CypA, B and C levels were measured followed by induced inflammatory conditions. After activation of T lymphocytes by incubation with concanavalin A, both intra and extracellular Cyps levels and the CD147 membrane receptor expression were increased leading to cell migration towards circulating CypA and CypB as chemoattractants. When CypA was modulated by natural and synthetic compounds, the inflammatory cascade was avoided including T cell migration. Our results strengthen the relationship between CypA, B, and C, their receptor, and the inflammatory process in human T lymphocytes, associating CypC with these cells for the first time.
Sandra Gegunde; Amparo Alfonso; Rebeca Alvariño; Eva Alonso; Luis M. Botana. Cyclophilins A, B, and C Role in Human T Lymphocytes Upon Inflammatory Conditions. Frontiers in Immunology 2021, 12, 1 .
AMA StyleSandra Gegunde, Amparo Alfonso, Rebeca Alvariño, Eva Alonso, Luis M. Botana. Cyclophilins A, B, and C Role in Human T Lymphocytes Upon Inflammatory Conditions. Frontiers in Immunology. 2021; 12 ():1.
Chicago/Turabian StyleSandra Gegunde; Amparo Alfonso; Rebeca Alvariño; Eva Alonso; Luis M. Botana. 2021. "Cyclophilins A, B, and C Role in Human T Lymphocytes Upon Inflammatory Conditions." Frontiers in Immunology 12, no. : 1.
A simple method for the quantification of 40 mycotoxins in milk was developed. This method is based on a QuEChERS extraction followed by the ultra-high liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) detection, and allows the simultaneous analysis of regulated, emerging, and modified compounds. A sample treatment procedure was optimized to include a concentration step for the analysis of some compounds such as aflatoxin M1. The method was in-house validated in terms of limits of detection (LODs), limits of quantification (LOQs), linearity, recoveries, and precision. LOQs lower than 10 ng/mL were obtained, and recoveries ranged from 61% to 120% with a precision, expressed as the relative standard deviation, lower than 15%. Therefore, acceptable performance characteristics were obtained fulfilling European regulations. The method was successfully applied for the quantification of mycotoxins in raw milk. It can be highlighted high occurrence of beauvericin and enniatins were found in low amounts.
Jesús M. González-Jartín; Inés Rodríguez-Cañás; Amparo Alfonso; María J. Sainz; Mercedes R. Vieytes; Ana Gomes; Isabel Ramos; Luis M. Botana. Multianalyte method for the determination of regulated, emerging and modified mycotoxins in milk: QuEChERS extraction followed by UHPLC–MS/MS analysis. Food Chemistry 2021, 356, 129647 .
AMA StyleJesús M. González-Jartín, Inés Rodríguez-Cañás, Amparo Alfonso, María J. Sainz, Mercedes R. Vieytes, Ana Gomes, Isabel Ramos, Luis M. Botana. Multianalyte method for the determination of regulated, emerging and modified mycotoxins in milk: QuEChERS extraction followed by UHPLC–MS/MS analysis. Food Chemistry. 2021; 356 ():129647.
Chicago/Turabian StyleJesús M. González-Jartín; Inés Rodríguez-Cañás; Amparo Alfonso; María J. Sainz; Mercedes R. Vieytes; Ana Gomes; Isabel Ramos; Luis M. Botana. 2021. "Multianalyte method for the determination of regulated, emerging and modified mycotoxins in milk: QuEChERS extraction followed by UHPLC–MS/MS analysis." Food Chemistry 356, no. : 129647.
Marine natural products have exhibited uncommon chemical structures with relevant antitumor properties highlighting their potential to inspire the development of new anticancer agents. The goal of this work was to study the antitumor activities of the brominated diterpene sphaerodactylomelol, a rare example of the dactylomelane family. Cytotoxicity (10–100 µM; 24 h) was evaluated on tumor cells (A549, CACO-2, HCT-15, MCF-7, NCI-H226, PC-3, SH-SY5Y, SK-ML-28) and the effects estimated by MTT assay. Hydrogen peroxide (H2O2) levels and apoptosis biomarkers (membrane translocation of phosphatidylserine, depolarization of mitochondrial membrane potential, Caspase-9 activity, and DNA condensation and/or fragmentation) were studied in the breast adenocarcinoma cellular model (MCF-7) and its genotoxicity on mouse fibroblasts (L929). Sphaerodactylomelol displayed an IC50 range between 33.04 and 89.41 µM without selective activity for a specific tumor tissue. The cells’ viability decrease was accompanied by an increase on H2O2 production, a depolarization of mitochondrial membrane potential and an increase of Caspase-9 activity and DNA fragmentation. However, the DNA damage studies in L929 non-malignant cell line suggested that this compound is not genotoxic for normal fibroblasts. Overall, the results suggest that the cytotoxicity of sphaerodactylomelol seems to be mediated by an increase of H2O2 levels and downstream apoptosis.
Celso Alves; Joana Silva; Susete Pinteus; Eva Alonso; Rebeca Alvariño; Adriana Duarte; Diorge Marmitt; Márcia Goettert; Helena Gaspar; Amparo Alfonso; Maria Alpoim; Luis M. Botana; Rui Pedrosa. Cytotoxic Mechanism of Sphaerodactylomelol, an Uncommon Bromoditerpene Isolated from Sphaerococcus coronopifolius. Molecules 2021, 26, 1374 .
AMA StyleCelso Alves, Joana Silva, Susete Pinteus, Eva Alonso, Rebeca Alvariño, Adriana Duarte, Diorge Marmitt, Márcia Goettert, Helena Gaspar, Amparo Alfonso, Maria Alpoim, Luis M. Botana, Rui Pedrosa. Cytotoxic Mechanism of Sphaerodactylomelol, an Uncommon Bromoditerpene Isolated from Sphaerococcus coronopifolius. Molecules. 2021; 26 (5):1374.
Chicago/Turabian StyleCelso Alves; Joana Silva; Susete Pinteus; Eva Alonso; Rebeca Alvariño; Adriana Duarte; Diorge Marmitt; Márcia Goettert; Helena Gaspar; Amparo Alfonso; Maria Alpoim; Luis M. Botana; Rui Pedrosa. 2021. "Cytotoxic Mechanism of Sphaerodactylomelol, an Uncommon Bromoditerpene Isolated from Sphaerococcus coronopifolius." Molecules 26, no. 5: 1374.
Parkinsons Disease (PD) is the second most common neurodegenerative disease worldwide, and is characterized by a progressive degeneration of dopaminergic neurons. Without an effective treatment, it is crucial to find new therapeutic options to fight the neurodegenerative process, which may arise from marine resources. Accordingly, the goal of the present work was to evaluate the ability of the monoterpenoid lactone Loliolide, isolated from the green seaweed Codium tomentosum, to prevent neurological cell death mediated by the neurotoxin 6-hydroxydopamine (6-OHDA) on SH-SY5Y cells and their anti-inflammatory effects in RAW 264.7 macrophages. Loliolide was obtained from the diethyl ether extract, purified through column chromatography and identified by NMR spectroscopy. The neuroprotective effects were evaluated by the MTT method. Cells´ exposure to 6-OHDA in the presence of Loliolide led to an increase of cells´ viability in 40%, and this effect was mediated by mitochondrial protection, reduction of oxidative stress condition and apoptosis, and inhibition of the NF-kB pathway. Additionally, Loliolide also suppressed nitric oxide production and inhibited the production of TNF-α and IL-6 pro-inflammatory cytokines. The results suggest that Loliolide can inspire the development of new neuroprotective therapeutic agents and thus, more detailed studies should be considered to validate its pharmacological potential.
Joana Silva; Celso Alves; Alice Martins; Patrícia Susano; Marco Simões; Miguel Guedes; Stephanie Rehfeldt; Susete Pinteus; Helena Gaspar; Américo Rodrigues; Márcia Goettert; Amparo Alfonso; Rui Pedrosa. Loliolide, a New Therapeutic Option for Neurological Diseases? In Vitro Neuroprotective and Anti-Inflammatory Activities of a Monoterpenoid Lactone Isolated from Codium tomentosum. International Journal of Molecular Sciences 2021, 22, 1888 .
AMA StyleJoana Silva, Celso Alves, Alice Martins, Patrícia Susano, Marco Simões, Miguel Guedes, Stephanie Rehfeldt, Susete Pinteus, Helena Gaspar, Américo Rodrigues, Márcia Goettert, Amparo Alfonso, Rui Pedrosa. Loliolide, a New Therapeutic Option for Neurological Diseases? In Vitro Neuroprotective and Anti-Inflammatory Activities of a Monoterpenoid Lactone Isolated from Codium tomentosum. International Journal of Molecular Sciences. 2021; 22 (4):1888.
Chicago/Turabian StyleJoana Silva; Celso Alves; Alice Martins; Patrícia Susano; Marco Simões; Miguel Guedes; Stephanie Rehfeldt; Susete Pinteus; Helena Gaspar; Américo Rodrigues; Márcia Goettert; Amparo Alfonso; Rui Pedrosa. 2021. "Loliolide, a New Therapeutic Option for Neurological Diseases? In Vitro Neuroprotective and Anti-Inflammatory Activities of a Monoterpenoid Lactone Isolated from Codium tomentosum." International Journal of Molecular Sciences 22, no. 4: 1888.
Cardiovascular diseases and atherosclerosis are currently some of the most widespread diseases of our time. Within cardiovascular disease, coronary artery disease and underlying atherosclerosis were recently linked with systemic and local inflammation. Cyclophilins participate in the initiation and progression of these inflammatory-related diseases. Cyclophilins are released into the extracellular space upon inflammatory stimuli and participate in the pathology of cardiovascular diseases. The cell surface receptor for extracellular cyclophilins, the CD147 receptor, also contributes to coronary artery disease pathogenesis. Nevertheless, the physiological relevance of cyclophilin's family and their receptor in cardiovascular diseases remains unclear. The present study aimed to better understand the role of cyclophilins in cardiovascular artery disease and their relationship with inflammation. Hence, cyclophilins and pro-inflammatory interleukins were measured in the serum of 30 subjects (divided into three groups according to coronary artery disease status: 10 patients with acute coronary syndrome, 10 patients with chronic coronary artery disease, and 10 control volunteers). In addition, cyclophilin levels and CD147 receptor expression were measured in T lymphocytes purified from these subjects. Cyclophilin A, B, and C, pro-inflammatory interleukins, and CD147 membrane expression were significantly elevated in patients with coronary artery disease.
Sandra Gegunde; Amparo Alfonso; Rebeca Alvariño; Eva Alonso; Carlos González-Juanatey; Luis M. Botana. Crosstalk between cyclophilins and T lymphocytes in coronary artery disease. Experimental Cell Research 2021, 400, 112514 .
AMA StyleSandra Gegunde, Amparo Alfonso, Rebeca Alvariño, Eva Alonso, Carlos González-Juanatey, Luis M. Botana. Crosstalk between cyclophilins and T lymphocytes in coronary artery disease. Experimental Cell Research. 2021; 400 (2):112514.
Chicago/Turabian StyleSandra Gegunde; Amparo Alfonso; Rebeca Alvariño; Eva Alonso; Carlos González-Juanatey; Luis M. Botana. 2021. "Crosstalk between cyclophilins and T lymphocytes in coronary artery disease." Experimental Cell Research 400, no. 2: 112514.
Since the potential anticancer activity of auranofin was discovered, gold compounds have attracted interest with a view to developing anticancer agents that follow cytotoxic mechanisms other than cisplatin. Two benzimidazole gold(I) derivatives containing triphenylphosphine (Au(pben)(PPh3)) (1) or triethylphosphine (Au(pben)(PEt3)) (2) were prepared and characterized by standard techniques. X-ray crystal structures for 1 and 2 were solved. The cytotoxicity of 1 and 2 was tested in human neuroblastoma SH-SY5Y cells. Cells were incubated with compounds for 24 h with concentrations ranging from 10 µM to 1 nM, and the half-maximal inhibitory concentration (IC50) was determined. 1 and 2 showed an IC50 of 2.7 and 1.6 µM, respectively. In order to better understand the type of cell death induced by compounds, neuroblastoma cells were stained with Annexin-FITC and propidium iodide. The fluorescence analysis revealed that compounds were inducing apoptosis; however, pre-treatment with the caspase inhibitor Z-VAD did not reduce cell death. Analysis of compound effects on caspase-3 activity and reactive oxygen species (ROS) production in SH-SY5Y cells revealed an antiproliferative ability mediated through oxidative stress and both caspase-dependent and caspase-independent mechanisms.
Lara Rouco; Ángeles Sánchez-González; Rebeca Alvariño; Amparo Alfonso; Ezequiel M. Vázquez-López; Emilia García-Martínez; Marcelino Maneiro. Combined Effect of Caspase-Dependent and Caspase-Independent Apoptosis in the Anticancer Activity of Gold Complexes with Phosphine and Benzimidazole Derivatives. Pharmaceuticals 2020, 14, 10 .
AMA StyleLara Rouco, Ángeles Sánchez-González, Rebeca Alvariño, Amparo Alfonso, Ezequiel M. Vázquez-López, Emilia García-Martínez, Marcelino Maneiro. Combined Effect of Caspase-Dependent and Caspase-Independent Apoptosis in the Anticancer Activity of Gold Complexes with Phosphine and Benzimidazole Derivatives. Pharmaceuticals. 2020; 14 (1):10.
Chicago/Turabian StyleLara Rouco; Ángeles Sánchez-González; Rebeca Alvariño; Amparo Alfonso; Ezequiel M. Vázquez-López; Emilia García-Martínez; Marcelino Maneiro. 2020. "Combined Effect of Caspase-Dependent and Caspase-Independent Apoptosis in the Anticancer Activity of Gold Complexes with Phosphine and Benzimidazole Derivatives." Pharmaceuticals 14, no. 1: 10.
The oceans harbor a great reservoir of molecules with unknown bioactivities, which could be useful for the treatment of illnesses that nowadays have no cure, such as neurodegenerative diseases. In this work, we evaluated the neuroprotective potential of the marine Fijian compounds tavarua deoxyriboside A and jasplakinolide against oxidative stress and neuroinflammation, crucial mechanisms in neurodegeneration. Both metabolites protected SH-SY5Y human neuroblastoma cells from H2O2 damage, improving mitochondrial function and activating the antioxidant systems of cells. These effects were mediated by their ability of inducing Nrf2 translocation. In BV2 microglial cells activated with lipopolysaccharide, Fijian metabolites also displayed promising results, decreasing the release of proinflammatory mediators (ROS, NO, cytokines) through the reduction of gp91 and NFkB–p65 expression. Finally, we performed a coculture among both cell lines, in which treatment with compounds protected SH-SY5Y cells from activated microglia, corroborating their neuroprotective effects. These results suggest that tavarua deoxyriboside A and jasplakinolide could be used as candidate molecules for further studies against neurodegeneration.
Rebeca Alvariño; Eva Alonso; Jioji N. Tabudravu; Nadia Pérez-Fuentes; Amparo Alfonso; Luis M. Botana. Tavarua Deoxyriboside A and Jasplakinolide as Potential Neuroprotective Agents: Effects on Cellular Models of Oxidative Stress and Neuroinflammation. ACS Chemical Neuroscience 2020, 12, 150 -162.
AMA StyleRebeca Alvariño, Eva Alonso, Jioji N. Tabudravu, Nadia Pérez-Fuentes, Amparo Alfonso, Luis M. Botana. Tavarua Deoxyriboside A and Jasplakinolide as Potential Neuroprotective Agents: Effects on Cellular Models of Oxidative Stress and Neuroinflammation. ACS Chemical Neuroscience. 2020; 12 (1):150-162.
Chicago/Turabian StyleRebeca Alvariño; Eva Alonso; Jioji N. Tabudravu; Nadia Pérez-Fuentes; Amparo Alfonso; Luis M. Botana. 2020. "Tavarua Deoxyriboside A and Jasplakinolide as Potential Neuroprotective Agents: Effects on Cellular Models of Oxidative Stress and Neuroinflammation." ACS Chemical Neuroscience 12, no. 1: 150-162.
Parkinson’s disease (PD) is the second most common neurodegenerative disorder, and is characterized by a progressive degeneration of the dopaminergic neurons in the substantianigra. Although not completely understood, several abnormal cellular events are known to be related with PD progression, such as oxidative stress, mitochondrial dysfunction and apoptosis. Accordingly, the aim of this study was to evaluate the neuroprotective effects of Codium tomentosum enriched fractions in a neurotoxicity model mediated by 6-hydroxydopamine (6-OHDA) on SH-SY5Y human cells, and the disclosure of their mechanisms of action. Additionally, a preliminary chemical screening of the most promising bioactive fractions of C. tomentosum was carried out by GC-MS analysis. Among the tested fractions, four samples exhibited the capacity to revert the neurotoxicity induced by 6-OHDA to values higher or similar to the vitamin E (90.11 ± 3.74% of viable cells). The neuroprotective effects were mediated by the mitigation of reactive oxygen species (ROS) generation, mitochondrial dysfunctions and DNA damage, together with the reduction of Caspase-3 activity. Compounds belonging to different chemical classes, such as terpenes, alcohols, carboxylic acids, aldehydes, esters, ketones, saturated and unsaturated hydrocarbons were tentatively identified by GC-MS. The results show that C. tomentosum is a relevant source of neuroprotective agents, with particular interest for preventive therapeutics.
Joana Silva; Alice Martins; Celso Alves; Susete Pinteus; Helena Gaspar; Amparo Alfonso; Rui Pedrosa. Natural Approaches for Neurological Disorders—The Neuroprotective Potential of Codium tomentosum. Molecules 2020, 25, 5478 .
AMA StyleJoana Silva, Alice Martins, Celso Alves, Susete Pinteus, Helena Gaspar, Amparo Alfonso, Rui Pedrosa. Natural Approaches for Neurological Disorders—The Neuroprotective Potential of Codium tomentosum. Molecules. 2020; 25 (22):5478.
Chicago/Turabian StyleJoana Silva; Alice Martins; Celso Alves; Susete Pinteus; Helena Gaspar; Amparo Alfonso; Rui Pedrosa. 2020. "Natural Approaches for Neurological Disorders—The Neuroprotective Potential of Codium tomentosum." Molecules 25, no. 22: 5478.
A new multi-mycotoxin analysis method was developed to identify and quantify 22 mycotoxins in multiple feed matrices. This method is based on a QuEChERS (quick, easy, cheap, effective, rugged and safe) extraction procedure followed by the ultra-high liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) detection. The QuEChERS extraction procedure was optimized for minimizing the matrix effect of maize. Obtained recoveries ranged from 67% to 94% and low LOQs (from 0.22 to 32.64 µg/kg) for regulated and emerging mycotoxins were obtained. Then, the method was expanded to seven raw materials and eight feedstuffs. In these matrices, the recovery for most mycotoxins was also high enough to fulfill the current legislation. The developed method was used for the analysis of 75 samples obtained from a nearby feed factory. Maize and maize-based products showed the highest occurrence of mycotoxins, although always below the legal limit. In addition, the ability of spheres of different composition and size to eliminate mycotoxins from raw materials and feedstuffs was tested. Up to 28% of the mycotoxin content can be removed from matrices by using glass spheres of 2 mm of diameter. Therefore, a new process for the physical removal of mycotoxins was developed.
Jesús M. González-Jartín; Amparo Alfonso; María J. Sainz; Mercedes R. Vieytes; Luis M. Botana. Multi-detection method for mycotoxins with a modified QuEChERS extraction in feed and development of a simple detoxification procedure. Animal Feed Science and Technology 2020, 272, 114745 .
AMA StyleJesús M. González-Jartín, Amparo Alfonso, María J. Sainz, Mercedes R. Vieytes, Luis M. Botana. Multi-detection method for mycotoxins with a modified QuEChERS extraction in feed and development of a simple detoxification procedure. Animal Feed Science and Technology. 2020; 272 ():114745.
Chicago/Turabian StyleJesús M. González-Jartín; Amparo Alfonso; María J. Sainz; Mercedes R. Vieytes; Luis M. Botana. 2020. "Multi-detection method for mycotoxins with a modified QuEChERS extraction in feed and development of a simple detoxification procedure." Animal Feed Science and Technology 272, no. : 114745.
In the last decades, due to monitoring programs and strict legislation poisoning incidents occurrence provoked by ingestion of naturally contaminated marine organisms has decreased. However, climate change and anthropogenic interference contributed to the expansion and establishment of toxic alien species to more temperate ecosystems. In this work, the coasts of Madeira, São Miguel islands and the northwestern Moroccan coast were surveyed for four groups of lipophilic toxins (yessotoxins, azaspiracids, pectenotoxins, and spirolides), searching for new vectors and geographical tendencies. Twenty-four species benthic organisms were screened using UHPLC-MS/MS technique. We report 19 new vectors for these toxins, six of them with commercial interest (P. aspera, P. ordinaria, C. lampas, P. pollicipes, H. tuberculata and P. lividus). Regarding toxin uptake a south-north gradient was detected. This study contributes to the update of monitoring routines and legislation policies, comprising a wider range of vectors, to better serve consumers and ecosystems preservation.
Marisa Silva; Inés Rodríguez; Aldo Barreiro; Manfred Kaufmann; Ana Isabel Neto; Meryem Hassouani; Brahim Sabour; Amparo Alfonso; Luis M. Botana; Vitor Vasconcelos. Lipophilic toxins occurrence in non-traditional invertebrate vectors from North Atlantic Waters (Azores, Madeira, and Morocco): Update on geographical tendencies and new challenges for monitoring routines. Marine Pollution Bulletin 2020, 161, 111725 .
AMA StyleMarisa Silva, Inés Rodríguez, Aldo Barreiro, Manfred Kaufmann, Ana Isabel Neto, Meryem Hassouani, Brahim Sabour, Amparo Alfonso, Luis M. Botana, Vitor Vasconcelos. Lipophilic toxins occurrence in non-traditional invertebrate vectors from North Atlantic Waters (Azores, Madeira, and Morocco): Update on geographical tendencies and new challenges for monitoring routines. Marine Pollution Bulletin. 2020; 161 ():111725.
Chicago/Turabian StyleMarisa Silva; Inés Rodríguez; Aldo Barreiro; Manfred Kaufmann; Ana Isabel Neto; Meryem Hassouani; Brahim Sabour; Amparo Alfonso; Luis M. Botana; Vitor Vasconcelos. 2020. "Lipophilic toxins occurrence in non-traditional invertebrate vectors from North Atlantic Waters (Azores, Madeira, and Morocco): Update on geographical tendencies and new challenges for monitoring routines." Marine Pollution Bulletin 161, no. : 111725.
The chemical investigation of the sponge Stylissa aff. carteri collected around Futuna Islands in the Pacific Ocean led to the isolation of three new dimeric pyrrole 2-aminoimidazole alkaloids (PIAs). Futunamine (1) features an unprecedented pyrrolo[1,2-c]imidazole core, while two other new dimeric PIAs were identified as analogues of palau’amine. Together with other known PIAs isolated from this species, they were shown to exhibit anti-inflammatory and neuroprotective activities.
Maria Miguel-Gordo; Sandra Gegunde; Laurence K. Jennings; Grégory Genta-Jouve; Kevin Calabro; Amparo Alfonso; Luis M. Botana; Olivier P. Thomas. Futunamine, a Pyrrole–Imidazole Alkaloid from the Sponge Stylissa aff. carteri Collected off the Futuna Islands. Journal of Natural Products 2020, 83, 2299 -2304.
AMA StyleMaria Miguel-Gordo, Sandra Gegunde, Laurence K. Jennings, Grégory Genta-Jouve, Kevin Calabro, Amparo Alfonso, Luis M. Botana, Olivier P. Thomas. Futunamine, a Pyrrole–Imidazole Alkaloid from the Sponge Stylissa aff. carteri Collected off the Futuna Islands. Journal of Natural Products. 2020; 83 (7):2299-2304.
Chicago/Turabian StyleMaria Miguel-Gordo; Sandra Gegunde; Laurence K. Jennings; Grégory Genta-Jouve; Kevin Calabro; Amparo Alfonso; Luis M. Botana; Olivier P. Thomas. 2020. "Futunamine, a Pyrrole–Imidazole Alkaloid from the Sponge Stylissa aff. carteri Collected off the Futuna Islands." Journal of Natural Products 83, no. 7: 2299-2304.
Marine and freshwater toxins contaminate water resources, shellfish and aquaculture products, causing a broad range of toxic effects in humans and animals. Different core-shell nanoparticles were tested as a new sorbent for removing marine and freshwater toxins from liquid media. Water solutions were contaminated with 20 μg/L of marine toxins and up to 50 μg/L of freshwater toxins and subsequently treated with 250 or 125 mg/L of nanoparticles. Under these conditions, carbon nanoparticles removed around 70% of saxitoxins, spirolides, and azaspiracids, and up to 38% of diarrheic shellfish poisoning toxins. In the case of freshwater toxins, the 85% of microcystin LR was eliminated; other cyclic peptide toxins were also removed in a high percentage. Marine toxins were adsorbed in the first 5 min of contact, while for freshwater toxins it was necessary 60 min to reach the maximum adsorption. Toxins were recovered by extraction from nanoparticles with different solvents. Gymnodinium catenatum, Prorocentrum lima, and Microcystis aeruginosa cultures were employed to test the ability of nanoparticles to adsorb toxins in a real environment, and the same efficacy to remove toxins was observed in these conditions. These results suggest the possibility of using the nanotechnology in the treatment of contaminated water or in chemical analysis applications.
Jesús M. González-Jartín; Lisandra De Castro Alves; Amparo Alfonso; Y. Piñeiro; Susana Yáñez Vilar; Inés Rodríguez; Manuel González Gomez; Zulema Vargas Osorio; María J. Sainz; Mercedes R. Vieytes; J. Rivas; Luis M. Botana. Magnetic nanostructures for marine and freshwater toxins removal. Chemosphere 2020, 256, 127019 .
AMA StyleJesús M. González-Jartín, Lisandra De Castro Alves, Amparo Alfonso, Y. Piñeiro, Susana Yáñez Vilar, Inés Rodríguez, Manuel González Gomez, Zulema Vargas Osorio, María J. Sainz, Mercedes R. Vieytes, J. Rivas, Luis M. Botana. Magnetic nanostructures for marine and freshwater toxins removal. Chemosphere. 2020; 256 ():127019.
Chicago/Turabian StyleJesús M. González-Jartín; Lisandra De Castro Alves; Amparo Alfonso; Y. Piñeiro; Susana Yáñez Vilar; Inés Rodríguez; Manuel González Gomez; Zulema Vargas Osorio; María J. Sainz; Mercedes R. Vieytes; J. Rivas; Luis M. Botana. 2020. "Magnetic nanostructures for marine and freshwater toxins removal." Chemosphere 256, no. : 127019.
Lara Rouco; Andrea Liberato; M. Jesús Fernández-Trujillo; Angeles Máñez; Manuel García Basallote; Rebeca Alvariño; Amparo Alfonso; Luis M. Botana; Marcelino Maneiro. Salen‑manganese complexes for controlling ROS damage: Neuroprotective effects, antioxidant activity and kinetic studies. Journal of Inorganic Biochemistry 2020, 203, 1 .
AMA StyleLara Rouco, Andrea Liberato, M. Jesús Fernández-Trujillo, Angeles Máñez, Manuel García Basallote, Rebeca Alvariño, Amparo Alfonso, Luis M. Botana, Marcelino Maneiro. Salen‑manganese complexes for controlling ROS damage: Neuroprotective effects, antioxidant activity and kinetic studies. Journal of Inorganic Biochemistry. 2020; 203 ():1.
Chicago/Turabian StyleLara Rouco; Andrea Liberato; M. Jesús Fernández-Trujillo; Angeles Máñez; Manuel García Basallote; Rebeca Alvariño; Amparo Alfonso; Luis M. Botana; Marcelino Maneiro. 2020. "Salen‑manganese complexes for controlling ROS damage: Neuroprotective effects, antioxidant activity and kinetic studies." Journal of Inorganic Biochemistry 203, no. : 1.
Background: Cyclophilins (Cyps) are a family of peptidyl-prolyl cis/trans isomerases consistently involved in cardiovascular diseases through the inflammation pathway. This study aims to investigate the serum levels of Cyps (CypA, CypB, CypC and CypD) in patients with coronary artery disease (CAD) and the correlation with clinical characteristics and inflammation parameters.Methods: We developed an observational prospective study with a total of 125 subjects: 40 patients with acute CAD, 40 patients with chronic CAD and 45 control volunteers, in whom serum levels of Cyps (CypA, CypB, CypC and CypD), interleukins and metalloproteinases were measured.Results: CypA levels increased significantly in CAD patients compared with control subjects, but no differences were noted between acute CAD (7.80 ± 1.30 ng/mL) and chronic CAD (5.52 ± 0.76 ng/mL) patients (P = 0.13). No differences in CypB and CypD levels were showed between CAD patients and controls and between acute CAD and chronic CAD patients. In relation with CypC, the levels in CAD patients were significantly higher compared to controls (32.42 ± 3.71 pg/mL vs. 9.38 ± 1.51 pg/mL, P < 0.001), but no differences between acute and chronic CAD groups were obtained (P = 0.62). We analyzed the CypC > 17.5 pg/mL cut-off point, and it was significantly associated with older age, hypertension, dyslipidemia and more extensive CAD in acute and chronic CAD groups.Conclusions: CypA and CypC levels are increased in CAD patients. High CypC serum levels could be a novel biomarker in CAD patients correlating with a more severe disease.Cardiol Res. 2020;11(5):319-327doi: https://doi.org/10.14740/cr1120
Jeremias Bayon; Amparo Alfonso; Sandra Gegunde; Eva Alonso; Rebeca Alvarino; Melisa Santas-Alvarez; Ana Testa-Fernandez; Ramon Rios-Vazquez; Luis Botana; Carlos Gonzalez-Juanatey. Cyclophilins in Ischemic Heart Disease: Differences Between Acute and Chronic Coronary Artery Disease Patients. Cardiology Research 2020, 11, 319 -327.
AMA StyleJeremias Bayon, Amparo Alfonso, Sandra Gegunde, Eva Alonso, Rebeca Alvarino, Melisa Santas-Alvarez, Ana Testa-Fernandez, Ramon Rios-Vazquez, Luis Botana, Carlos Gonzalez-Juanatey. Cyclophilins in Ischemic Heart Disease: Differences Between Acute and Chronic Coronary Artery Disease Patients. Cardiology Research. 2020; 11 (5):319-327.
Chicago/Turabian StyleJeremias Bayon; Amparo Alfonso; Sandra Gegunde; Eva Alonso; Rebeca Alvarino; Melisa Santas-Alvarez; Ana Testa-Fernandez; Ramon Rios-Vazquez; Luis Botana; Carlos Gonzalez-Juanatey. 2020. "Cyclophilins in Ischemic Heart Disease: Differences Between Acute and Chronic Coronary Artery Disease Patients." Cardiology Research 11, no. 5: 319-327.
Scope Fruit‐derived drinks consumption is considered beneficial due to the antioxidant and neuroprotective effects of polyphenols separately, but studies including their total constituents are scarce. In this work, we determined the antioxidant and anti‐inflammatory neuroprotective effects of apple‐derived beverages in a mouse model of LPS‐induced inflammation. Methods and results Preliminary antioxidant and neuroinflammatory experiments were carried out with fifteen drink polyphenolic extracts in SH‐SY5Y and BV2 cells, using H2O2 as pro‐oxidant and LPS as pro‐inflammatory stimulus, respectively. Extracts improved antioxidant systems functioning and presented neuroprotective mitochondrial‐related effects. In microglia, extracts reduced reactive oxygen species and modulated cytokine release. To better mimic human consumption, four concentrated dealcoholized apple‐derived drinks (three ciders and apple juice) were supplied to mice for seven days in substitution of drinking water. Mice treated with beverages presented reduced brain oxidative stress and inflammatory markers (lipid peroxidation, NO, iNOS, TNF‐α) after LPS injection. Interestingly, genetic expression of antioxidant enzymes and glutathione levels were also greatly augmented after drink intake. Conclusion Our results confirm the antioxidant and anti‐inflammatory‐mediated neuroprotective properties of apple‐derived drinks, suggesting that their consumption could be a good approach for prevention of neurodegenerative disorders. To our knowledge, this is the first description of cider neuroprotective effects. This article is protected by copyright. All rights reserved
Rebeca Alvariño; Eva Alonso; Amparo Alfonso; Luis M. Botana. Neuroprotective Effects of Apple‐Derived Drinks in a Mice Model of Inflammation. Molecular Nutrition & Food Research 2019, 64, 1 .
AMA StyleRebeca Alvariño, Eva Alonso, Amparo Alfonso, Luis M. Botana. Neuroprotective Effects of Apple‐Derived Drinks in a Mice Model of Inflammation. Molecular Nutrition & Food Research. 2019; 64 (2):1.
Chicago/Turabian StyleRebeca Alvariño; Eva Alonso; Amparo Alfonso; Luis M. Botana. 2019. "Neuroprotective Effects of Apple‐Derived Drinks in a Mice Model of Inflammation." Molecular Nutrition & Food Research 64, no. 2: 1.
Gracilins are diterpenes derivative, isolated from the marine sponge Spongionella gracilis. Natural gracilins and synthetic derivatives have shown antioxidant, immunosuppressive, and neuroprotective capacities related to the affinity for cyclophilins. The aim of this work was to study anti-inflammatory and immunosuppressive pathways modulated by gracilin L and two synthetic analogues, compound 1 and 2, on a cellular model of inflammation. In this way, the murine BV2 microglia cell line was used. To carry out the experiments, microglia cells were pre-treated with compounds for 1 h and then stimulated with lipopolysaccharide for 24 h to determine reactive oxygen species production, mitochondrial membrane potential, the release of nitric oxide, interleukin-6 and tumor necrosis factor-α and the expression of Nuclear factor-erythroid 2-related factor 2, Nuclear Factor-κB, the inducible nitric oxide synthase, and the cyclophilin A. Finally, a co-culture of neuron SH-SY5Y and microglia BV2 cells was used to check the neuroprotective effect of these compounds. Cyclosporine A was used as a control of effect. The compounds were able to decrease inflammatory mediators, the expression of inflammatory target proteins as well as they activated anti-oxidative mechanism upon inflammatory conditions. For this reason, natural and synthetic gracilins could be interesting for developing anti-inflammatory drugs.
Sandra Gegunde; Amparo Alfonso; Eva Alonso; Rebeca Alvariño; Luis M. Botana. Gracilin-Derivatives as Lead Compounds for Anti-inflammatory Effects. Cellular and Molecular Neurobiology 2019, 40, 603 -615.
AMA StyleSandra Gegunde, Amparo Alfonso, Eva Alonso, Rebeca Alvariño, Luis M. Botana. Gracilin-Derivatives as Lead Compounds for Anti-inflammatory Effects. Cellular and Molecular Neurobiology. 2019; 40 (4):603-615.
Chicago/Turabian StyleSandra Gegunde; Amparo Alfonso; Eva Alonso; Rebeca Alvariño; Luis M. Botana. 2019. "Gracilin-Derivatives as Lead Compounds for Anti-inflammatory Effects." Cellular and Molecular Neurobiology 40, no. 4: 603-615.
The search for compounds capable of targeting early pathological changes of Alzheimer̀s disease (AD), such as oxidative stress and neuroinflammation, is an important challenge. Gracilin A derivatives were recently synthesized, using a pharmacophore-directed retrosynthesis (PDR) strategy, and found to possess potent neuroprotective effects. In this work, the previously described derivatives 1–7 which demonstrated mitochondrial-mediated, antioxidant effects were chosen for further study. The ability of compounds to modulate the expression of antioxidant genes (CAT, GPx, SODs, and Nrf2) was determined in SH-SY5Y cells, and the simplified derivatives 2 and 3 were found to be the most effective. The anti-neuroinflammatory properties of all derivatives were assessed in BV2 microglial cells activated with lipopolysaccharide (LPS). Several derivatives decreased the release of cytokines (Il-1β, IL-6, GM-CSF, and TNF-α) and other damaging molecules (ROS, NO) and also regulated the translocation of Nrf2 and NFκB, and reduced p38 activation. These protective effects were confirmed in a trans-well coculture with BV2 and SH-SY5Y cells and several derivatives increased SH-SY5Y survival. This present work demonstrates the neuroprotective properties of gracilin A derivatives, making them promising candidate drugs for AD. Particularly, derivatives 2 and 3 showed the greatest potential as lead compounds for further development.
Rebeca Alvariño; Eva Alonso; Mikail E. Abbasov; Christian Michael Chaheine; Michael L. Conner; Daniel Romo; Amparo Alfonso; Luis M. Botana. Gracilin A Derivatives Target Early Events in Alzheimer’s Disease: in Vitro Effects on Neuroinflammation and Oxidative Stress. ACS Chemical Neuroscience 2019, 10, 4102 -4111.
AMA StyleRebeca Alvariño, Eva Alonso, Mikail E. Abbasov, Christian Michael Chaheine, Michael L. Conner, Daniel Romo, Amparo Alfonso, Luis M. Botana. Gracilin A Derivatives Target Early Events in Alzheimer’s Disease: in Vitro Effects on Neuroinflammation and Oxidative Stress. ACS Chemical Neuroscience. 2019; 10 (9):4102-4111.
Chicago/Turabian StyleRebeca Alvariño; Eva Alonso; Mikail E. Abbasov; Christian Michael Chaheine; Michael L. Conner; Daniel Romo; Amparo Alfonso; Luis M. Botana. 2019. "Gracilin A Derivatives Target Early Events in Alzheimer’s Disease: in Vitro Effects on Neuroinflammation and Oxidative Stress." ACS Chemical Neuroscience 10, no. 9: 4102-4111.
Amparo Alfonso; Jeremías Bayón; Sandra Gegunde; Eva Alonso; Rebeca Alvariño; Melisa Santás-Álvarez; Ana Testa-Fernández; Ramón Rios-Vázquez; Carlos González-Juanatey; Luis M. Botana. High Serum Cyclophilin C levels as a risk factor marker for Coronary Artery Disease. Scientific Reports 2019, 9, 1 .
AMA StyleAmparo Alfonso, Jeremías Bayón, Sandra Gegunde, Eva Alonso, Rebeca Alvariño, Melisa Santás-Álvarez, Ana Testa-Fernández, Ramón Rios-Vázquez, Carlos González-Juanatey, Luis M. Botana. High Serum Cyclophilin C levels as a risk factor marker for Coronary Artery Disease. Scientific Reports. 2019; 9 (1):1.
Chicago/Turabian StyleAmparo Alfonso; Jeremías Bayón; Sandra Gegunde; Eva Alonso; Rebeca Alvariño; Melisa Santás-Álvarez; Ana Testa-Fernández; Ramón Rios-Vázquez; Carlos González-Juanatey; Luis M. Botana. 2019. "High Serum Cyclophilin C levels as a risk factor marker for Coronary Artery Disease." Scientific Reports 9, no. 1: 1.