A laccase-producing ascomycete fungus was isolated from soil collected around the premises of a textile dye factory and identified as Nectriella pironii. Efficient laccase production was achieved via the synergistic action of 1 mM copper sulfate and ferulic acid. Extracts of rapeseed oil cake, grass hay, and leaf litter collected in a pocket urban park were used for enzyme production. The highest laccase activity (3,330 U/L) was observed in the culture grown on the leaf litter extract. This is the first report on biosynthesis of laccase by N. pironii. This is also the first study on utilization of naturally fallen park leaves as a substrate for fungal laccase production. The extracellular enzyme possessing laccase activity was purified to homogeneity by ion-exchange and gel filtration chromatographic techniques. The amino acid sequence of the protein revealed highest similarity to the laccase enzyme produced by Stachybotrys chartarum—and considerable homology to those produced by other fungal species. The purified laccase possessed a molecular mass of 50 kDa. The enzyme had an optimum pH of 2.0 or 6.0 and retained more than 50% of residual activity after 3 hours of incubation at pH 3.0–10.6 or 4.0–9.0 when 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid or 2,6-dimethoxyphenol, respectively, were used. Dithiothreitol, β-mercaptoethanol, and sodium azide at 1 mM concentration strongly inhibited the laccase activity, while in the presence of 50 mM urea, the enzyme was found to retain 25% of its activity. The laccase was able to decolorize more than 80% of Indigo Carmine, Remazol Brilliant Blue R, Reactive Orange 16, and Acid Red 27 dyes within 1 h. The possibility of leaf litter use for the production of the laccase enzyme from N. pironii (IM 6443), exhibiting high pH stability and degradative potential, makes it a promising tool for use in different environmental and industrial operations.

This article presents a pilot study investigating the multidimensional diversity of cemeteries as an important element of cultural heritage and green infrastructure within the urban landscape. We studied the state and diversity of nature, perceptions, and activities of visitors. As religion is an important aspect that differentiates cemeteries from each other, we studied a sample of four multi-confessional urban cemeteries in Łódź (Poland) and Leipzig (Germany) by using site observation and a questionnaire survey. We found that cemeteries are far undervalued as public green resources that can perform important functions in sociocultural life and the mental well-being of the general public, as the perceptions of silence- and contemplation-seeking visitors tell us. The perception of cemeteries depends on the level of secularization, varying from a sacrum sphere up to specific recreational and touristic opportunities; findings that should be considered by town planners when optimizing the cultural ecosystem services of green spaces.

The present study described analyses of two similar informal recreational green areas (former constructional waste disposal landfills) in two large cities (Warsaw city and Łódź city). On the basis of local society’s opinions, the land use conditions related to current accessibility, management, safety, cleanliness, variety and diversity of facility and vegetation, neighbourhood and connections with the urban green infrastructure of the given sites were studied. Overall feedback posted by the site users indicated that, despite temporary land use, both sites are good leisure areas that provide cultural ecosystem services to the citizens. However, their undefined development makes them to varying degrees neglected and risky spaces, especially for the local community. The reflection of the results of the present study may help the local authorities to manage the spaces of former landfills in accordance with the needs of the local society as well as define new functions of informal urban green space in the sustainable spatial policy in post-socialist cities in Poland and Eastern Europe.

[This corrects the article DOI: 10.1371/journal.pone.0231453.].

Urban environments face escalating challenges due to uncontrolled urbanization, rapid population growth, and climate changes, prompting the exploration of sustainable solutions for enhancing urban green spaces (UGSs). For this reason, poplars (Populus L.), due to their rapid growth, wide range adaptability to environmental conditions and versatility of use, have emerged as very promising. This comprehensive review synthesizes current knowledge regarding poplar’s application in urban landscapes, emphasizing its multifaceted contributions and benefits. However, challenges arise from the variable lifespans of different poplar cultivars, necessitating strategic management approaches. Selecting cultivars based on growth rates, root system characteristics, and adaptability to urban conditions is pivotal. Adaptive replanting strategies, incorporating species with varying lifespans, offer solutions to maintain continual greenery in urban landscapes. Collaborative efforts between researchers, urban planners, and policymakers are essential for devising comprehensive strategies that maximize benefits while addressing challenges associated with their variable lifespans. In conclusion, harnessing poplar’s potential in urban greenery initiatives requires a balanced approach that capitalizes on their benefits while mitigating challenges. Further research and adaptive strategies are crucial for sustained and effective utilization to create resilient and vibrant urban landscapes.