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Komivi Dossa
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, and Rural Affairs, Wuhan, China

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Review
Published: 22 June 2021 in BMC Plant Biology
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Background Sesame is a rare example of non-model and minor crop for which numerous genetic loci and candidate genes underlying features of interest have been disclosed at relatively high resolution. These progresses have been achieved thanks to the applications of the genome-wide association study (GWAS) approach. GWAS has benefited from the availability of high-quality genomes, re-sequencing data from thousands of genotypes, extensive transcriptome sequencing, development of haplotype map and web-based functional databases in sesame. Results In this paper, we reviewed the GWAS methods, the underlying statistical models and the applications for genetic discovery of important traits in sesame. A novel online database SiGeDiD (http://sigedid.ucad.sn/) has been developed to provide access to all genetic and genomic discoveries through GWAS in sesame. We also tested for the first time, applications of various new GWAS multi-locus models in sesame. Conclusions Collectively, this work portrays steps and provides guidelines for efficient GWAS implementation in sesame, a non-model crop.

ACS Style

Muez Berhe; Komivi Dossa; Jun You; Pape Adama Mboup; Idrissa Navel Diallo; Diaga Diouf; Xiurong Zhang; Linhai Wang. Genome-wide association study and its applications in the non-model crop Sesamum indicum. BMC Plant Biology 2021, 21, 1 -19.

AMA Style

Muez Berhe, Komivi Dossa, Jun You, Pape Adama Mboup, Idrissa Navel Diallo, Diaga Diouf, Xiurong Zhang, Linhai Wang. Genome-wide association study and its applications in the non-model crop Sesamum indicum. BMC Plant Biology. 2021; 21 (1):1-19.

Chicago/Turabian Style

Muez Berhe; Komivi Dossa; Jun You; Pape Adama Mboup; Idrissa Navel Diallo; Diaga Diouf; Xiurong Zhang; Linhai Wang. 2021. "Genome-wide association study and its applications in the non-model crop Sesamum indicum." BMC Plant Biology 21, no. 1: 1-19.

Journal article
Published: 02 June 2021 in Plants
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Ethiopia is one of the centers of genetic diversity of sesame (Sesamum indicum L.). The sesame genetic resources present in the country should be explored for local, regional, and international genetic improvement programs to design high-performing and market-preferred varieties. This study’s objective was to determine the extent of genetic variation among 100 diverse cultivated sesame germplasm collections of Ethiopia using phenotypic traits and simple sequence repeat (SSR) markers to select distinct and complementary genotypes for breeding. One hundred sesame entries were field evaluated at two locations in Ethiopia for agro-morphological traits and seed oil content using a 10 × 10 lattice design with two replications. Test genotypes were profiled using 27 polymorphic SSR markers at the Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences. Analysis of variance revealed significant (p ≤ 0.05) entry by environment interaction for plant height, internode length, number of secondary branches, and grain yield. Genotypes such as Hirhir Kebabo Hairless-9, Setit-3, Orofalc ACC-2, Hirhir Humera Sel-6, ABX = 2-01-2, and Setit-1 recorded grain yield of >0.73 ton ha−1 with excellent performance in yield component such as oil yield per hectare. Grain yield had positive and significant (p< 0.01) associations with oil yield (r = 0.99), useful for simultaneous selection for yield improvement in sesame. The SSR markers revealed gene diversity and polymorphic information content values of 0.30 and 0.25, respectively, showing that the tested sesame accessions were genetically diverse. Cluster analysis resolved the accessions into two groups, while population structure analysis revealed four major heterotic groups, thus enabling selection and subsequent crossing to develop breeding populations for cultivar development. Based on phenotypic and genomic divergence, the following superior and complementary genotypes: Hirhir Humera Sel-6, Setit-3, Hirhir Kebabo Hairless Sel-4, Hirhir Nigara 1st Sel-1, Humera-1 and Hirhir Kebabo Early Sel-1 (from cluster II-a), Hirhir kebabo hairless-9, NN-0029(2), NN0068-2 and Bawnji Fiyel Kolet, (from cluster II-b). The selected genotypes will serve as parents in the local breeding program in Ethiopia.

ACS Style

Desawi Teklu; Hussein Shimelis; Abush Tesfaye; Jacob Mashilo; Xiurong Zhang; Yanxin Zhang; Komivi Dossa; Admire Shayanowako. Genetic Variability and Population Structure of Ethiopian Sesame (Sesamum indicum L.) Germplasm Assessed through Phenotypic Traits and Simple Sequence Repeats Markers. Plants 2021, 10, 1129 .

AMA Style

Desawi Teklu, Hussein Shimelis, Abush Tesfaye, Jacob Mashilo, Xiurong Zhang, Yanxin Zhang, Komivi Dossa, Admire Shayanowako. Genetic Variability and Population Structure of Ethiopian Sesame (Sesamum indicum L.) Germplasm Assessed through Phenotypic Traits and Simple Sequence Repeats Markers. Plants. 2021; 10 (6):1129.

Chicago/Turabian Style

Desawi Teklu; Hussein Shimelis; Abush Tesfaye; Jacob Mashilo; Xiurong Zhang; Yanxin Zhang; Komivi Dossa; Admire Shayanowako. 2021. "Genetic Variability and Population Structure of Ethiopian Sesame (Sesamum indicum L.) Germplasm Assessed through Phenotypic Traits and Simple Sequence Repeats Markers." Plants 10, no. 6: 1129.

Journal article
Published: 18 May 2021 in International Journal of Molecular Sciences
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The biosynthesis and storage of lipids in oil crop seeds involve many gene families, such as nonspecific lipid-transfer proteins (nsLTPs). nsLTPs are cysteine-rich small basic proteins essential for plant development and survival. However, in sesame, information related to nsLTPs was limited. Thus, the objectives of this study were to identify the Sesamum indicum nsLTPs (SiLTPs) and reveal their potential role in oil accumulation in sesame seeds. Genome-wide analysis revealed 52 SiLTPs, nonrandomly distributed on 10 chromosomes in the sesame variety Zhongzhi 13. Following recent classification methods, the SiLTPs were divided into nine types, among which types I and XI were the dominants. We found that the SiLTPs could interact with several transcription factors, including APETALA2 (AP2), DNA binding with one finger (Dof), etc. Transcriptome analysis showed a tissue-specific expression of some SiLTP genes. By integrating the SiLTPs expression profiles and the weighted gene co-expression network analysis (WGCNA) results of two contrasting oil content sesame varieties, we identified SiLTPI.23 and SiLTPI.28 as the candidate genes for high oil content in sesame seeds. The presumed functions of the candidate gene were validated through overexpression of SiLTPI.23 in Arabidopsis thaliana. These findings expand our knowledge on nsLTPs in sesame and provide resources for functional studies and genetic improvement of oil content in sesame seeds.

ACS Style

Shengnan Song; Jun You; Lisong Shi; Chen Sheng; Wangyi Zhou; Senouwa Dossou; Komivi Dossa; Linhai Wang; Xiurong Zhang. Genome-Wide Analysis of nsLTP Gene Family and Identification of SiLTPs Contributing to High Oil Accumulation in Sesame (Sesamum indicum L.). International Journal of Molecular Sciences 2021, 22, 5291 .

AMA Style

Shengnan Song, Jun You, Lisong Shi, Chen Sheng, Wangyi Zhou, Senouwa Dossou, Komivi Dossa, Linhai Wang, Xiurong Zhang. Genome-Wide Analysis of nsLTP Gene Family and Identification of SiLTPs Contributing to High Oil Accumulation in Sesame (Sesamum indicum L.). International Journal of Molecular Sciences. 2021; 22 (10):5291.

Chicago/Turabian Style

Shengnan Song; Jun You; Lisong Shi; Chen Sheng; Wangyi Zhou; Senouwa Dossou; Komivi Dossa; Linhai Wang; Xiurong Zhang. 2021. "Genome-Wide Analysis of nsLTP Gene Family and Identification of SiLTPs Contributing to High Oil Accumulation in Sesame (Sesamum indicum L.)." International Journal of Molecular Sciences 22, no. 10: 5291.

Journal article
Published: 30 January 2021 in Plants
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Sesame is one of the oldest oil crops in the world and is widely grown in the tropical and subtropical areas of Asia, Africa and America. Upon the completion of the sesame reference genome version 1.0, we launched Sinbase 1.0 as an integrated database for genomic and bioinformatics analyses. Recently, an upgraded version (version 2.0) of the genome sequence was released. In addition, large numbers of multi-omics data have been generated on sesame, but a comprehensive database that integrates these resources for the community has been lacking until now. Here, we developed an interactive and comprehensive sesame multi-omics database, Sinbase 2.0, which provides information of the sesame updated genome containing 13 chromosomes, 3 genetic linkage maps, 5 intra- and 6 inter-species comparative genomics, 1 genomic variation analysis, 5 transcriptome data, 1 proteome, 31 functional markers, 175 putative functional genes, and 54 QTLs detected for important agronomic traits. Moreover, Sinbase 2.0 has been enriched with novel user-friendly computational tools. All datasets of Sinbase 2.0 can be downloaded online conveniently. Sinbase 2.0 will be updated regularly with new available sesame multi-omics data and can be accessed freely via Sinbase 2.—Sesame Muti-Omics Database. We expect that Sinbase 2.0, similarly to the previous version, will continue to make a major contribution to advance sesame research towards a better understanding of its biology and genetic improvement, as well as comparative genomics and evolutionary biology.

ACS Style

Liwen Wang; Jingyin Yu; Yanxin Zhang; Jun You; Xiurong Zhang; Linhai Wang. Sinbase 2.0: An Updated Database to Study Multi-Omics in Sesamum indicum. Plants 2021, 10, 272 .

AMA Style

Liwen Wang, Jingyin Yu, Yanxin Zhang, Jun You, Xiurong Zhang, Linhai Wang. Sinbase 2.0: An Updated Database to Study Multi-Omics in Sesamum indicum. Plants. 2021; 10 (2):272.

Chicago/Turabian Style

Liwen Wang; Jingyin Yu; Yanxin Zhang; Jun You; Xiurong Zhang; Linhai Wang. 2021. "Sinbase 2.0: An Updated Database to Study Multi-Omics in Sesamum indicum." Plants 10, no. 2: 272.

Research article
Published: 28 December 2020 in Plant Biotechnology Journal
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Developing crops with improved root system is crucial in current global warming scenario. Underexploited crops are valuable reservoirs of unique genes that can be harnessed for the improvement of major crops. In this study, we performed genome‐wide association studies on seven root traits in sesame (Sesamum indicum L.) and uncovered 409 significant signals, 19 quantitative trait loci containing 32 candidate genes. A peak SNP significantly associated with root number and root dry weight traits was located in the promoter of the gene named ‘Big Root Biomass’ (BRB), which was subsequently validated in a bi‐parental population. BRB has no functional annotation and is restricted to the Lamiales order. We detected the presence of a novel motif ‘AACACACAC’ located in the 5’‐UTR of BRB in single and duplicated copy in accessions with high and small root biomass, respectively. A strong expression level of BRB was negatively correlated with high root biomass and this was attributed to the gene SiMYB181 which represses the activity of BRB by binding specifically to the single motif but not to the duplicated one. Curiously, the allele that enhanced BRB expression has been intensively selected by modern breeding. Overexpression of BRB in Arabidopsis modulates auxin pathway leading to reduced root biomass, improved yield parameters under normal growth conditions and increased drought stress sensitivity. Overall, BRB represents a solid gene model for improving the performance of sesame and other crops.

ACS Style

Komivi Dossa; Rong Zhou; Donghua Li; Aili Liu; Lu Qin; Marie A. Mmadi; Ruqi Su; Yujuan Zhang; Jianqiang Wang; Yuan Gao; Xiurong Zhang; Jun You. A novel motif in the 5’‐UTR of an orphan gene ‘ Big Root Biomass ’ modulates root biomass in sesame. Plant Biotechnology Journal 2020, 19, 1065 -1079.

AMA Style

Komivi Dossa, Rong Zhou, Donghua Li, Aili Liu, Lu Qin, Marie A. Mmadi, Ruqi Su, Yujuan Zhang, Jianqiang Wang, Yuan Gao, Xiurong Zhang, Jun You. A novel motif in the 5’‐UTR of an orphan gene ‘ Big Root Biomass ’ modulates root biomass in sesame. Plant Biotechnology Journal. 2020; 19 (5):1065-1079.

Chicago/Turabian Style

Komivi Dossa; Rong Zhou; Donghua Li; Aili Liu; Lu Qin; Marie A. Mmadi; Ruqi Su; Yujuan Zhang; Jianqiang Wang; Yuan Gao; Xiurong Zhang; Jun You. 2020. "A novel motif in the 5’‐UTR of an orphan gene ‘ Big Root Biomass ’ modulates root biomass in sesame." Plant Biotechnology Journal 19, no. 5: 1065-1079.

Journal article
Published: 25 November 2020 in Genes
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Seed coat color is a crucial agronomic trait in sesame (Sesamum indicum L.) since it is strongly linked to seed oil, proteins, and lignans contents, and also influences consumer preferences. In East Asia, black sesame seed is used in the treatment and the prevention of various diseases. However, in sesame, little is known about the establishment of the seed coat color, and only one gene has been reported to control black pigmentation. This study provides an overview of developing seeds transcriptome of two varieties of sesame “Zhongfengzhi No.1” (white seed) and “Zhongzhi No.33” (black seed) and shed light on genes involving in black seed formation. Until eight days post-anthesis (DPA), both the seeds of the two varieties were white. The black sesame seed turned to yellow between 9 and 11 DPA and then black between 12 and 14 DPA. The black and white sesame showed similar trend-expressed genes with the numbers increased at the early stages of seed development. The differentially expressed genes (DEGs) number increased with seed development in the two sesame varieties. We examined the DEGs and uncovered that more were up-regulated at the early stages. The DEGs between the black and white sesame were mainly enriched in 37 metabolic pathways, among which the flavonoid biosynthesis and biosynthesis of secondary metabolites were dominants. Furthermore, we identified 20 candidate genes associated with pigment biosynthesis in black sesame seed, among which 10 were flavonoid biosynthesis and regulatory genes. These genes also include isochorismate and polyphenol oxidase genes. By comparing the phenotypes and genes expressions of the black and white sesame seed at different development stages, this work revealed the important role of 8–14 DPA in black pigment biosynthesis and accumulation. Moreover, it unfolded candidate genes associated with black pigmentation in sesame. These findings provide a vast transcriptome dataset and list of genes that will be targeted for functional studies related to the molecular mechanism involved in biosynthesis and regulation of seed coat color in sesame.

ACS Style

Linhai Wang; Senouwa Segla Koffi Dossou; Xin Wei; Yanxin Zhang; Donghua Li; Jingyin Yu; Xiurong Zhang. Transcriptome Dynamics during Black and White Sesame (Sesamum indicum L.) Seed Development and Identification of Candidate Genes Associated with Black Pigmentation. Genes 2020, 11, 1399 .

AMA Style

Linhai Wang, Senouwa Segla Koffi Dossou, Xin Wei, Yanxin Zhang, Donghua Li, Jingyin Yu, Xiurong Zhang. Transcriptome Dynamics during Black and White Sesame (Sesamum indicum L.) Seed Development and Identification of Candidate Genes Associated with Black Pigmentation. Genes. 2020; 11 (12):1399.

Chicago/Turabian Style

Linhai Wang; Senouwa Segla Koffi Dossou; Xin Wei; Yanxin Zhang; Donghua Li; Jingyin Yu; Xiurong Zhang. 2020. "Transcriptome Dynamics during Black and White Sesame (Sesamum indicum L.) Seed Development and Identification of Candidate Genes Associated with Black Pigmentation." Genes 11, no. 12: 1399.

Data article
Published: 31 July 2020 in Data in Brief
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Soil salinity is a major abiotic factor affecting the growth and development of important crops such as sesame (Sesamum indicum L.). To understand the molecular mechanisms of this oilseed crop in response to salt stress, we examined the transcriptome and proteome profiles of two sesame varieties, with contrasting tolerances to salinity. Here, RNA sequencing and quantitative proteomic analyses of 30 samples from salt-tolerant and -sensitive sesame seedlings under salt stress were carried out. These data can be used for differential gene expression and protein accumulation analyses, based on a genetic aberration or phenotypic differences in sesame responses to salt stress. Our dataset provides an extensive resource for understanding the molecular mechanisms underlying the adaptation of sesame to salt stress, and may constitute useful a resource for increasing the tolerance of major crop plants to raised salinity levels in soils.

ACS Style

Yujuan Zhang; Donghua Li; Rong Zhou; Aili Liu; Linhai Wang; Yanxin Zhang; Huihui Gong; Xiurong Zhang; Jun You. A collection of transcriptomic and proteomic datasets from sesame in response to salt stress. Data in Brief 2020, 32, 106096 .

AMA Style

Yujuan Zhang, Donghua Li, Rong Zhou, Aili Liu, Linhai Wang, Yanxin Zhang, Huihui Gong, Xiurong Zhang, Jun You. A collection of transcriptomic and proteomic datasets from sesame in response to salt stress. Data in Brief. 2020; 32 ():106096.

Chicago/Turabian Style

Yujuan Zhang; Donghua Li; Rong Zhou; Aili Liu; Linhai Wang; Yanxin Zhang; Huihui Gong; Xiurong Zhang; Jun You. 2020. "A collection of transcriptomic and proteomic datasets from sesame in response to salt stress." Data in Brief 32, no. : 106096.

Journal article
Published: 24 December 2019 in AoB PLANTS
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An increasing number of candidate genes related to abiotic stress tolerance are being discovered and proposed to improve the existing cultivars of the high oil-bearing crop sesame (Sesamum indicum L.). However, the in planta functional validation of these genes is remarkably lacking. In this study, we cloned a novel sesame R2-R3 MYB gene SiMYB75 which is strongly induced by drought, sodium chloride (NaCl), abscisic acid (ABA) and mannitol. SiMYB75 is expressed in various sesame tissues, especially in root and its protein is predicted to be located in the nucleus. Ectopic over-expression of SiMYB75 in Arabidopsis notably promoted root growth and improved plant tolerance to drought, NaCl and mannitol treatments. Furthermore, SiMYB75 over-expressing lines accumulated higher content of ABA than wild-type plants under stresses and also increased sensitivity to ABA. Physiological analyses revealed that SiMYB75 confers abiotic stress tolerance by promoting stomatal closure to reduce water loss; inducing a strong reactive oxygen species scavenging activity to alleviate cell damage and apoptosis; and also, up-regulating the expression levels of various stress-marker genes in the ABA-dependent pathways. Our data suggested that SiMYB75 positively modulates drought, salt and osmotic stresses responses through ABA-mediated pathways. Thus, SiMYB75 could be a promising candidate gene for the improvement of abiotic stress tolerance in crop species including sesame.

ACS Style

Komivi Dossa; Marie A Mmadi; Rong Zhou; Aili Liu; Yuanxiao Yang; Diaga Diouf; Jun You; Xiurong Zhang. Ectopic expression of the sesame MYB transcription factor SiMYB305 promotes root growth and modulates ABA-mediated tolerance to drought and salt stresses in Arabidopsis. AoB PLANTS 2019, 12, plz081 .

AMA Style

Komivi Dossa, Marie A Mmadi, Rong Zhou, Aili Liu, Yuanxiao Yang, Diaga Diouf, Jun You, Xiurong Zhang. Ectopic expression of the sesame MYB transcription factor SiMYB305 promotes root growth and modulates ABA-mediated tolerance to drought and salt stresses in Arabidopsis. AoB PLANTS. 2019; 12 (1):plz081.

Chicago/Turabian Style

Komivi Dossa; Marie A Mmadi; Rong Zhou; Aili Liu; Yuanxiao Yang; Diaga Diouf; Jun You; Xiurong Zhang. 2019. "Ectopic expression of the sesame MYB transcription factor SiMYB305 promotes root growth and modulates ABA-mediated tolerance to drought and salt stresses in Arabidopsis." AoB PLANTS 12, no. 1: plz081.

Communication
Published: 17 September 2019 in Genes
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Rice, being a major staple food crop and sensitive to salinity conditions, bears heavy yield losses due to saline soil. Although some salt responsive genes have been identified in rice, their applications in developing salt tolerant cultivars have resulted in limited achievements. Herein, we used bioinformatic approaches to perform a meta-analysis of three transcriptome datasets from salinity and control conditions in order to reveal novel genes and the molecular pathways underlying rice response to salt. From a total of 28,432 expressed genes, we identify 457 core differentially expressed genes (DEGs) constitutively responding to salt, regardless of the stress duration, genotype, or the tissue. Gene co-expression analysis divided the core DEGs into three different modules, each of them contributing to salt response in a unique metabolic pathway. Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses highlighted key biological processes and metabolic pathways involved in the salt response. We identified important novel hub genes encoding proteins of different families including CAM, DUF630/632, DUF581, CHL27, PP2-13, LEA4-5, and transcription factors, which could be functionally characterized using reverse genetic experiments. This novel repertoire of candidate genes related to salt response in rice will be useful for engineering salt tolerant varieties.

ACS Style

Mingdong Zhu; Hongjun Xie; Xiangjin Wei; Komivi Dossa; Yaying Yu; Suozhen Hui; Guohua Tang; Xiaoshan Zeng; Yinghong Yu; Peisong Hu; Jianlong Wang. WGCNA Analysis of Salt-Responsive Core Transcriptome Identifies Novel Hub Genes in Rice. Genes 2019, 10, 719 .

AMA Style

Mingdong Zhu, Hongjun Xie, Xiangjin Wei, Komivi Dossa, Yaying Yu, Suozhen Hui, Guohua Tang, Xiaoshan Zeng, Yinghong Yu, Peisong Hu, Jianlong Wang. WGCNA Analysis of Salt-Responsive Core Transcriptome Identifies Novel Hub Genes in Rice. Genes. 2019; 10 (9):719.

Chicago/Turabian Style

Mingdong Zhu; Hongjun Xie; Xiangjin Wei; Komivi Dossa; Yaying Yu; Suozhen Hui; Guohua Tang; Xiaoshan Zeng; Yinghong Yu; Peisong Hu; Jianlong Wang. 2019. "WGCNA Analysis of Salt-Responsive Core Transcriptome Identifies Novel Hub Genes in Rice." Genes 10, no. 9: 719.

Journal article
Published: 13 August 2019 in International Journal of Molecular Sciences
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Sesame is a source of a healthy vegetable oil, attracting a growing interest worldwide. Abiotic stresses have devastating effects on sesame yield; hence, studies have been performed to understand sesame molecular responses to abiotic stresses, but the core abiotic stress-responsive genes (CARG) that the plant reuses in response to an array of environmental stresses are unknown. We performed a meta-analysis of 72 RNA-Seq datasets from drought, waterlogging, salt and osmotic stresses and identified 543 genes constantly and differentially expressed in response to all stresses, representing the sesame CARG. Weighted gene co-expression network analysis of the CARG revealed three functional modules controlled by key transcription factors. Except for salt stress, the modules were positively correlated with the abiotic stresses. Network topology of the modules showed several hub genes predicted to play prominent functions. As proof of concept, we generated over-expressing Arabidopsis lines with hub and non-hub genes. Transgenic plants performed better under drought, waterlogging, and osmotic stresses than the wild-type plants but did not tolerate the salt treatment. As expected, the hub gene was significantly more potent than the non-hub gene. Overall, we discovered several novel candidate genes, which will fuel investigations on plant responses to multiple abiotic stresses.

ACS Style

Komivi Dossa; Marie A. Mmadi; Rong Zhou; Tianyuan Zhang; Ruqi Su; Yujuan Zhang; Linhai Wang; Jun You; Xiurong Zhang. Depicting the Core Transcriptome Modulating Multiple Abiotic Stresses Responses in Sesame (Sesamum indicum L.). International Journal of Molecular Sciences 2019, 20, 3930 .

AMA Style

Komivi Dossa, Marie A. Mmadi, Rong Zhou, Tianyuan Zhang, Ruqi Su, Yujuan Zhang, Linhai Wang, Jun You, Xiurong Zhang. Depicting the Core Transcriptome Modulating Multiple Abiotic Stresses Responses in Sesame (Sesamum indicum L.). International Journal of Molecular Sciences. 2019; 20 (16):3930.

Chicago/Turabian Style

Komivi Dossa; Marie A. Mmadi; Rong Zhou; Tianyuan Zhang; Ruqi Su; Yujuan Zhang; Linhai Wang; Jun You; Xiurong Zhang. 2019. "Depicting the Core Transcriptome Modulating Multiple Abiotic Stresses Responses in Sesame (Sesamum indicum L.)." International Journal of Molecular Sciences 20, no. 16: 3930.

Journal article
Published: 31 July 2019 in Bioinformation
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Drought is one of the major abiotic stresses causing yield losses and restricted growing area for several major crops. Rice being a major staple food crop and sensitive to water-deficit conditions bears heavy yield losses due to drought stress. To breed drought tolerant rice cultivars, it is of interest to understand the mechanisms of drought tolerance. In this regard, large amount of publicly available transcriptome datasets could be utilized. In this study, we used different transcriptome datasets obtained under drought stress in comparison to normal conditions (control) to identify novel drought responsive genes and their underlying molecular mechanisms. We found 517 core drought responsive differentially expressed genes (DEGs) and different modules using gene co-expression analysis to specifically predict their biological roles in drought tolerance. Gene ontology and KEGG analyses showed key biological processes and metabolic pathways involved in drought tolerance. Further, network analysis pinpointed important hub DEGs and major transcription factors regulating the expression of drought responsive genes in each module. These identified novel DEGs and transcription factors could be functionally characterized using systems biology approaches, which can significantly enhance our knowledge about the molecular mechanisms of drought tolerance in rice.

ACS Style

Yanmei Lv; Lei Xu; Komivi Dossa; Kun Zhou; Mingdong Zhu; Hongjun Xie; Shanjun Tang; Yaying Yu; Xiayu Guo; Bin Zhou. Identification of putative drought-responsive genes in rice using gene co-expression analysis. Bioinformation 2019, 15, 480 -488.

AMA Style

Yanmei Lv, Lei Xu, Komivi Dossa, Kun Zhou, Mingdong Zhu, Hongjun Xie, Shanjun Tang, Yaying Yu, Xiayu Guo, Bin Zhou. Identification of putative drought-responsive genes in rice using gene co-expression analysis. Bioinformation. 2019; 15 (7):480-488.

Chicago/Turabian Style

Yanmei Lv; Lei Xu; Komivi Dossa; Kun Zhou; Mingdong Zhu; Hongjun Xie; Shanjun Tang; Yaying Yu; Xiayu Guo; Bin Zhou. 2019. "Identification of putative drought-responsive genes in rice using gene co-expression analysis." Bioinformation 15, no. 7: 480-488.

Original article
Published: 18 July 2019 in Planta
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Sesame harbors a large diversity in root morphological and anatomical traits and a high root biomass improves the plant aboveground biomass as well as the seed yield. Sesame provides one of the most nutritious and healthy vegetable oils, sparking an increasing demand of its seeds. However, with the low yield and productivity of sesame, there is still a huge gap between the seed demand and supply. Improving the root system has a high potential to increase crop productivity, but information on the diversity of the sesame root systems is still lacking. In this study, 40 diverse sesame varieties were grown in soil and hydroponics systems and the diversity of the root system was investigated. The results showed that sesame holds a large root morphological and anatomical diversity, which can be harnessed in breeding programmes. Based on the clustering of the genotypes in hydroponics and soil culture systems, we found that similar genotypes were commonly clustered either in the small-root or in the big-root group, indicating that the hydroponics system can be employed for a large-scale root phenotyping. Our results further revealed that the root biomass positively contributes to increased seed yield in sesame, based on multi-environmental trials. By comparing the root transcriptome of two contrasting genotypes, 2897 differentially expressed genes were detected and they were enriched in phenylpropanoid biosynthesis, starch and sucrose metabolism, stilbenoid, diarylheptanoid and gingerol biosynthesis, flavonoid biosynthesis, suggesting that these pathways are crucial for sesame root growth and development. Overall, this study sheds light on the diversity of sesame root system and offers the basis for improving root traits and increasing sesame seed yield.

ACS Style

Ruqi Su; Rong Zhou; Marie Ali Mmadi; Donghua Li; Lu Qin; Aili Liu; Jianqiang Wang; Yuan Gao; Mengyuan Wei; Lisong Shi; Ziming Wu; Jun You; Xiurong Zhang; Komivi Dossa. Root diversity in sesame (Sesamum indicum L.): insights into the morphological, anatomical and gene expression profiles. Planta 2019, 250, 1461 -1474.

AMA Style

Ruqi Su, Rong Zhou, Marie Ali Mmadi, Donghua Li, Lu Qin, Aili Liu, Jianqiang Wang, Yuan Gao, Mengyuan Wei, Lisong Shi, Ziming Wu, Jun You, Xiurong Zhang, Komivi Dossa. Root diversity in sesame (Sesamum indicum L.): insights into the morphological, anatomical and gene expression profiles. Planta. 2019; 250 (5):1461-1474.

Chicago/Turabian Style

Ruqi Su; Rong Zhou; Marie Ali Mmadi; Donghua Li; Lu Qin; Aili Liu; Jianqiang Wang; Yuan Gao; Mengyuan Wei; Lisong Shi; Ziming Wu; Jun You; Xiurong Zhang; Komivi Dossa. 2019. "Root diversity in sesame (Sesamum indicum L.): insights into the morphological, anatomical and gene expression profiles." Planta 250, no. 5: 1461-1474.

Journal article
Published: 20 June 2019 in BMC Plant Biology
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Sesame is an important oil crop due to its high oil, antioxidant, and protein content. Drought stress is a major abiotic stress that affects sesame production as well as the quality of sesame seed. To reveal the adaptive mechanism of sesame in response to water deficient conditions, transcriptomic and metabolomics were applied in drought-tolerant (DT) and drought-susceptible (DS) sesame genotypes. Transcriptomic analysis reveals a set of core drought-responsive genes (684 up-regulated and 1346 down-regulated) in sesame that was robustly differently expressed in both genotypes. Most enriched drought-responsive genes are mainly involved in protein processing in endoplasmic reticulum, plant hormone signal transduction photosynthesis, lipid metabolism, and amino acid metabolism. Drought-susceptible genotype was more disturbed by drought stress at both transcriptional and metabolic levels, since more drought-responsive genes/metabolites were identified in DS. Drought-responsive genes associated with stress response, amino acid metabolism, and reactive oxygen species scavenging were more enriched or activated in DT. According to the partial least-squares discriminate analysis, the most important metabolites which were accumulated under drought stress in both genotypes includes ABA, amino acids, and organic acids. Especially, higher levels of ABA, proline, arginine, lysine, aromatic and branched chain amino acids, GABA, saccharopine, 2-aminoadipate, and allantoin were found in DT under stress condition. Combination of transcriptomic and metabolomic analysis highlights the important role of amino acid metabolism (especially saccharopine pathway) and ABA metabolism and signaling pathway for drought tolerance in sesame. The results of the present study provide valuable information for better understanding the molecular mechanism underlying drought tolerance of sesame, and also provide useful clues for the genetic improvement of drought tolerance in sesame.

ACS Style

Jun You; Yujuan Zhang; Aili Liu; Donghua Li; Xiao Wang; Komivi Dossa; Rong Zhou; Jingyin Yu; Yanxin Zhang; Linhai Wang; Xiurong Zhang. Transcriptomic and metabolomic profiling of drought-tolerant and susceptible sesame genotypes in response to drought stress. BMC Plant Biology 2019, 19, 1 -16.

AMA Style

Jun You, Yujuan Zhang, Aili Liu, Donghua Li, Xiao Wang, Komivi Dossa, Rong Zhou, Jingyin Yu, Yanxin Zhang, Linhai Wang, Xiurong Zhang. Transcriptomic and metabolomic profiling of drought-tolerant and susceptible sesame genotypes in response to drought stress. BMC Plant Biology. 2019; 19 (1):1-16.

Chicago/Turabian Style

Jun You; Yujuan Zhang; Aili Liu; Donghua Li; Xiao Wang; Komivi Dossa; Rong Zhou; Jingyin Yu; Yanxin Zhang; Linhai Wang; Xiurong Zhang. 2019. "Transcriptomic and metabolomic profiling of drought-tolerant and susceptible sesame genotypes in response to drought stress." BMC Plant Biology 19, no. 1: 1-16.

Journal article
Published: 10 June 2019 in Annals of Botany
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Background and Aims Sulphur (S) is an essential macronutrient involved in numerous metabolic pathways required for plant growth. Crops of the plant family Brassicaceae require more S compared with other crops for optimum growth and yield, with most S ultimately sequestered in the mature seeds as the storage proteins cruciferin and napin, along with the unique S-rich secondary metabolite glucosinolate (GSL). It is well established that S assimilation primarily takes place in the shoots rather than roots, and that sulphate is the major form in which S is transported and stored in plants. We carried out a developmental S audit to establish the net fluxes of S in two lines of Brassica juncea mustard where seed GSL content differed but resulted in no yield penalty. Methods We quantified S pools (sulphate, GSL and total S) in different organs at multiple growth stages until maturity, which also allowed us to test the hypothesis that leaf S, accumulated as a primary S sink, becomes remobilized as a secondary source to meet the requirements of GSL as the dominant seed S sink. Key Results Maximum plant sulphate accumulation had occurred by floral initiation in both lines, at which time most of the sulphate was found in the leaves, confirming its role as the primary S sink. Up to 52 % of total sulphate accumulated by the low-GSL plants was lost through senesced leaves. In contrast, S from senescing leaves of the high-GSL line was remobilized to other tissues, with GSL accumulating in the seed from commencement of silique filling until maturity. Conclusion We have established that leaf S compounds that accumulated as primary S sinks at early developmental stages in condiment type B. juncea become remobilized as a secondary S source to meet the demand for GSL as the dominant seed S sink at maturity.

ACS Style

Priyakshee Borpatragohain; Terry J Rose; Lei Liu; Bronwyn J Barkla; Carolyn A Raymond; Graham J King. Remobilization and fate of sulphur in mustard. Annals of Botany 2019, 124, 471 -480.

AMA Style

Priyakshee Borpatragohain, Terry J Rose, Lei Liu, Bronwyn J Barkla, Carolyn A Raymond, Graham J King. Remobilization and fate of sulphur in mustard. Annals of Botany. 2019; 124 (3):471-480.

Chicago/Turabian Style

Priyakshee Borpatragohain; Terry J Rose; Lei Liu; Bronwyn J Barkla; Carolyn A Raymond; Graham J King. 2019. "Remobilization and fate of sulphur in mustard." Annals of Botany 124, no. 3: 471-480.

Journal article
Published: 16 May 2019 in BMC Genetics
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Sesame (Sesamum indicum) can accumulate over 60% oil in its seed. However, low oil content genotypes with an oil content of less than 50% are also observed. To gain insights into how genes shape this variation, we examined 22 seed and carpel transcriptomes from 3 varieties of sesame with high and low oil content. A total of 34.6~52.2% of the sesame genes were expressed with a RPKM greater than 5 in the 22 tissue samples. The expressed gene numbers tended to decrease in the seed but fluctuated in the carpels from 10 to 30 days post-anthesis (DPA). Compared with that of the low oil content sesames, the high oil content sesame exhibited more positive gene expression during seed development. Typically, genes involved in lipid biosynthesis were enriched and could distinguish the high and low genotypes at 30 DPA, suggesting the pivotal role of seed oil biosynthesis in the later stages. Key homologous lipid genes that function in TAG biosynthesis, including those that encoded glycerol-3-phosphate acyltransferase (GPAT), acyl-CoA:diacylglycerol acyltransferase (DGAT), and phospholipid:diacylglycerol acyltransferase (PDAT), were strengthened asynchronously at different stages, but the lipid transfer protein (LTP)-encoding genes, including SIN_1019175, SIN_1019172 and SIN_1010009, usually were highlighted in the high oil content sesames. Furthermore, a list of 23 candidate genes was identified and predicted to be beneficial for higher oil content accumulation. Despite the different gene expression patterns between the seeds and carpels, the two tissues showed a cooperative relationship during seed development, and biological processes, such as transport, catabolic process and small molecule metabolic process, changed synchronously. The study elucidated the different expression profiles in high and low oil content sesames and revealed key stages and a list of candidate genes that shaped oil content variation. These findings will accelerate dissection of the genetic mechanism of sesame oil biosynthesis.

ACS Style

Linhai Wang; Yanxin Zhang; Donghua Li; Komivi Dossa; Ming Li Wang; Rong Zhou; Jingyin Yu; Xiurong Zhang. Gene expression profiles that shape high and low oil content sesames. BMC Genetics 2019, 20, 45 .

AMA Style

Linhai Wang, Yanxin Zhang, Donghua Li, Komivi Dossa, Ming Li Wang, Rong Zhou, Jingyin Yu, Xiurong Zhang. Gene expression profiles that shape high and low oil content sesames. BMC Genetics. 2019; 20 (1):45.

Chicago/Turabian Style

Linhai Wang; Yanxin Zhang; Donghua Li; Komivi Dossa; Ming Li Wang; Rong Zhou; Jingyin Yu; Xiurong Zhang. 2019. "Gene expression profiles that shape high and low oil content sesames." BMC Genetics 20, no. 1: 45.

Journal article
Published: 19 April 2019 in Journal of Proteomics
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Sesame is one of the most important oilseed crops and has high nutritional value. The yield and quality of sesame are severely affected by high salinity in coastal and semi-arid/arid regions. In this study, the phenotypic, physiological, and proteomic changes induced by salt treatment were analyzed in salt-tolerant (G441) and salt-sensitive (G358) seedlings. Phenotypic and physiological results indicated that G441 had an enhanced capacity to withstand salinity stress compared to G358. Proteomic analysis revealed a strong induction of salt-responsive protein species in sesame, mainly related to catalytic, hydrolase, oxidoreductase, and binding activities. Pathway enrichment analysis showed that more salt-responsive proteins in G441 were involved in tyrosine metabolism, carbon fixation in photosynthetic organisms, carbon metabolism, alpha-linolenic acid metabolism, biosynthesis of amino acids, photosynthesis, and glutathione metabolism. Furthermore, G441 displayed unique differentially accumulated proteins in seedlings functioning as heat shock proteins, abscisic acid receptor PYL2-like, calcium-dependent protein kinases, serine/threonine-protein phosphatases, nucleoredoxin, and antioxidant enzymes. Quantitative real-time PCR analysis revealed that some of the proteins were also regulated by salinity stress at the transcript level. Our findings provide important information on salinity responses in plants and may constitute useful resources for enhancing salinity tolerance in sesame. Our study identified potential biological pathways and salt-responsive protein species related to transducing stress signals and scavenging reactive oxygen species under salt stress. These findings will provide possible participants/pathways/proteins that contribute to salt tolerance and may serve as the basis for improving salinity tolerance in sesame and other plants.

ACS Style

Yujuan Zhang; Mengyuan Wei; Aili Liu; Rong Zhou; Donghua Li; Komivi Dossa; Linhai Wang; Yanxin Zhang; Huihui Gong; Xiurong Zhang; Jun You. Comparative proteomic analysis of two sesame genotypes with contrasting salinity tolerance in response to salt stress. Journal of Proteomics 2019, 201, 73 -83.

AMA Style

Yujuan Zhang, Mengyuan Wei, Aili Liu, Rong Zhou, Donghua Li, Komivi Dossa, Linhai Wang, Yanxin Zhang, Huihui Gong, Xiurong Zhang, Jun You. Comparative proteomic analysis of two sesame genotypes with contrasting salinity tolerance in response to salt stress. Journal of Proteomics. 2019; 201 ():73-83.

Chicago/Turabian Style

Yujuan Zhang; Mengyuan Wei; Aili Liu; Rong Zhou; Donghua Li; Komivi Dossa; Linhai Wang; Yanxin Zhang; Huihui Gong; Xiurong Zhang; Jun You. 2019. "Comparative proteomic analysis of two sesame genotypes with contrasting salinity tolerance in response to salt stress." Journal of Proteomics 201, no. : 73-83.

Research article
Published: 22 February 2019 in Plant Biotechnology Journal
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Unlike most of the important food crops, sesame can survive drought but severe and repeated drought episodes, especially occurring during the reproductive stage, significantly curtail the productivity of this high oil crop. Genome‐wide association study was conducted for traits related to drought tolerance using 400 diverse sesame accessions, including landraces and modern cultivars. Ten stable QTLs explaining more than 40% of the phenotypic variation and located on four linkage groups were significantly associated with drought tolerance related traits. Accessions from the tropical area harbored higher numbers of drought tolerance alleles at the peak loci and were found to be more tolerant than those from the northern‐area, indicating a long‐term genetic adaptation to drought‐prone environments. We found that sesame has already fixed important alleles conferring survival to drought which may explain its relative high drought tolerance. However, most of the alleles crucial for productivity and yield maintenance under drought conditions are far from been fixed. This study also revealed that pyramiding the favorable alleles observed at the peak loci is of high potential for enhancing drought tolerance in sesame. In addition, our results highlighted two important pleiotropic QTLs harboring known and unreported drought tolerance genes such as SiABI4, SiTTM3, SiGOLS1, SiNIMIN1 and SiSAM. By integrating candidate gene association study, gene expression, and transgenic experiments, we demonstrated that SiSAM confers drought tolerance by modulating polyamine levels and ROS homeostasis, and a missense mutation in the coding region partly contributes to the natural variation of drought tolerance in sesame. This article is protected by copyright. All rights reserved.

ACS Style

Komivi Dossa; Donghua Li; Rong Zhou; Jingyin Yu; Linhai Wang; Yanxin Zhang; Jun You; Aili Liu; Marie A. Mmadi; Daniel Fonceka; Diaga Diouf; Ndiaga Cissé; Xin Wei; Xiurong Zhang. The genetic basis of drought tolerance in the high oil crop Sesamum indicum. Plant Biotechnology Journal 2019, 17, 1788 -1803.

AMA Style

Komivi Dossa, Donghua Li, Rong Zhou, Jingyin Yu, Linhai Wang, Yanxin Zhang, Jun You, Aili Liu, Marie A. Mmadi, Daniel Fonceka, Diaga Diouf, Ndiaga Cissé, Xin Wei, Xiurong Zhang. The genetic basis of drought tolerance in the high oil crop Sesamum indicum. Plant Biotechnology Journal. 2019; 17 (9):1788-1803.

Chicago/Turabian Style

Komivi Dossa; Donghua Li; Rong Zhou; Jingyin Yu; Linhai Wang; Yanxin Zhang; Jun You; Aili Liu; Marie A. Mmadi; Daniel Fonceka; Diaga Diouf; Ndiaga Cissé; Xin Wei; Xiurong Zhang. 2019. "The genetic basis of drought tolerance in the high oil crop Sesamum indicum." Plant Biotechnology Journal 17, no. 9: 1788-1803.

Journal article
Published: 11 February 2019 in BMC Plant Biology
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Soil salinity is one of the major serious factors that affect agricultural productivity of almost all crops worldwide, including the important oilseed crop sesame. In order to improve salinity resistance in sesame, it is crucial to understand the molecular mechanisms underlying the adaptive response to salinity stress. In the present study, two contrasting sesame genotypes differing in salt tolerance were used to decipher the adaptive responses to salt stress based on morphological, transcriptome and metabolome characterizations. Morphological results indicated that under salt stress, the salt-tolerant (ST) genotype has enhanced capacity to withstand salinity stress, higher seed germination rate and plant survival rate, as well as better growth rate than the salt-sensitive genotype. Transcriptome analysis revealed strongly induced salt-responsive genes in sesame mainly related to amino acid metabolism, carbohydrate metabolism, biosynthesis of secondary metabolites, plant hormone signal transduction, and oxidation-reduction process. Especially, several pathways were preferably enriched with differentially expressed genes in ST genotype, including alanine, aspartate and glutamate metabolism, carotenoid biosynthesis, galactose metabolism, glycolysis/gluconeogenesis, glyoxylate and dicarboxylate metabolism, porphyrin and chlorophyll metabolism. Metabolome profiling under salt stress showed a higher accumulation degree of metabolites involved in stress tolerance in ST, and further highlighted that the amino acid metabolism, and sucrose and raffinose family oligosaccharides metabolism were enhanced in ST. These findings suggest that the candidate genes and metabolites involved in crucial biological pathways may regulate salt tolerance of sesame, and increase our understanding of the molecular mechanisms underlying the adaptation of sesame to salt stress.

ACS Style

Yujuan Zhang; Donghua Li; Rong Zhou; Xiao Wang; Komivi Dossa; Linhai Wang; Yanxin Zhang; Jingyin Yu; Huihui Gong; Xiurong Zhang; Jun You. Transcriptome and metabolome analyses of two contrasting sesame genotypes reveal the crucial biological pathways involved in rapid adaptive response to salt stress. BMC Plant Biology 2019, 19, 1 -14.

AMA Style

Yujuan Zhang, Donghua Li, Rong Zhou, Xiao Wang, Komivi Dossa, Linhai Wang, Yanxin Zhang, Jingyin Yu, Huihui Gong, Xiurong Zhang, Jun You. Transcriptome and metabolome analyses of two contrasting sesame genotypes reveal the crucial biological pathways involved in rapid adaptive response to salt stress. BMC Plant Biology. 2019; 19 (1):1-14.

Chicago/Turabian Style

Yujuan Zhang; Donghua Li; Rong Zhou; Xiao Wang; Komivi Dossa; Linhai Wang; Yanxin Zhang; Jingyin Yu; Huihui Gong; Xiurong Zhang; Jun You. 2019. "Transcriptome and metabolome analyses of two contrasting sesame genotypes reveal the crucial biological pathways involved in rapid adaptive response to salt stress." BMC Plant Biology 19, no. 1: 1-14.

Original article
Published: 21 January 2019 in The Plant Journal
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Rapeseed (Brassica napus L.) is a model plant for polyploid crop research and the second‐leading source of vegetable oil worldwide. Silique length (SL) and seed weight (SW) are two important yield‐influencing traits in rapeseed. Using map‐based cloning, we isolated qSLWA9, which encodes a P450 monooxygenase (BnaA9.CYP78A9) and functions as a positive regulator of SL. The expression level of BnaA9.CYP78A9 in silique valves of long‐silique variety is much higher than that of regular‐silique variety, which results in elongated cells and a prolonged phase of silique elongation. Highly expressing BnaA9.CYP78A9 in long‐silique variety and transgenic plants had higher concentration of auxin in developing siliques, which induced a number of auxin ‐related genes but no genes in well‐known auxin biosynthesis pathways, suggesting that BnaA9.CYP78A9 may influence auxin concentration by affecting auxin metabolism or an unknown auxin biosynthesis pathway. A 3.7‐kb CACTA‐like transposable element (TE) inserted in the 3.9‐kb upstream regulatory sequence of BnaA9.CYP78A9 elevates the expression level, suggesting that the CACTA‐like TE acts as an enhancer to stimulate the high gene expression and silique elongation. Marker and sequence analysis revealed that the TE in B. napus had recently been introgressed from B. rapa by interspecific hybridization. The insertion of the TE is consistently associated with long‐siliques and large‐seeds in both B. napus and B. rapa collections. However, the frequency of the CACTA‐like TE in rapeseed varieties is still very low, suggesting that this allele has not been widely used in rapeseed breeding program and would be invaluable for yield improvement in rapeseed breeding. This article is protected by copyright. All rights reserved.

ACS Style

Liuliu Shi; Jurong Song; Chaocheng Guo; Bo Wang; Zhilin Guan; Pu Yang; Xun Chen; Qinghua Zhang; Graham J. King; Jing Wang; Kede Liu. ACACTA‐like transposable element in the upstream region ofBnaA9.CYP78A9acts as an enhancer to increase silique length and seed weight in rapeseed. The Plant Journal 2019, 98, 524 -539.

AMA Style

Liuliu Shi, Jurong Song, Chaocheng Guo, Bo Wang, Zhilin Guan, Pu Yang, Xun Chen, Qinghua Zhang, Graham J. King, Jing Wang, Kede Liu. ACACTA‐like transposable element in the upstream region ofBnaA9.CYP78A9acts as an enhancer to increase silique length and seed weight in rapeseed. The Plant Journal. 2019; 98 (3):524-539.

Chicago/Turabian Style

Liuliu Shi; Jurong Song; Chaocheng Guo; Bo Wang; Zhilin Guan; Pu Yang; Xun Chen; Qinghua Zhang; Graham J. King; Jing Wang; Kede Liu. 2019. "ACACTA‐like transposable element in the upstream region ofBnaA9.CYP78A9acts as an enhancer to increase silique length and seed weight in rapeseed." The Plant Journal 98, no. 3: 524-539.

Journal article
Published: 10 December 2018 in BMC Plant Biology
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Sesame is a major oilseed crop which is widely cultivated all around the world. Flowering, the timing of transition from vegetative to reproductive growth, is one of the most important events in the life cycle of sesame. Sesame is a typical short-day (SD) plant and its flowering is largely affected by photoperiod. However, the flowering mechanism in sesame at the molecular level is still not very clear. Previous studies showed that the CONSTANS (CO) gene is the crucial photoperiod response gene which plays a center role in duration of the plant vegetative growth. In this study, the CO-like (COL) genes were identified and characterized in the sesame genome. Two homologs of the CO gene in the SiCOLs, SiCOL1 and SiCOL2, were recognized and comprehensively analyzed. However, sequence analysis showed that SiCOL2 lacked one of the B-box motifs. In addition, the flowering time of the transgenic Arabidopsis lines with overexpressed SiCOL2 were longer than that of SiCOL1, indicating that SiCOL1 was more likely to be the potential functional homologue of CO in sesame. Expression analysis revealed that SiCOL1 had high expressed levels before flowering in leaves and exhibited diurnal rhythmic expression in both SD and long-day (LD) conditions. In total, 16 haplotypes of SiCOL1 were discovered in the sesame collections from Asia. However, the mutated haplotypes did not express under both SD and LD conditions and was regarded as a nonfunctional allele. Notably, the sesame landraces from high-latitude regions harboring nonfunctional alleles of SiCOL1 flowered much earlier than landraces from low-latitude regions under LD condition, and adapted to the northernmost regions of sesame cultivation. The result indicated that sesame landraces from high-latitude regions might have undergone artificial selection to adapt to the LD environment. Our results suggested that SiCOL1 might contribute to regulation of flowering in sesame and natural variations in SiCOL1 were probably related to the expansion of sesame cultivation to high-latitude regions. The results could be used in sesame breeding and in broadening adaptation of sesame varieties to new regions.

ACS Style

Rong Zhou; Pan Liu; Donghua Li; Xiurong Zhang; Xin Wei. Photoperiod response-related gene SiCOL1 contributes to flowering in sesame. BMC Plant Biology 2018, 18, 343 .

AMA Style

Rong Zhou, Pan Liu, Donghua Li, Xiurong Zhang, Xin Wei. Photoperiod response-related gene SiCOL1 contributes to flowering in sesame. BMC Plant Biology. 2018; 18 (1):343.

Chicago/Turabian Style

Rong Zhou; Pan Liu; Donghua Li; Xiurong Zhang; Xin Wei. 2018. "Photoperiod response-related gene SiCOL1 contributes to flowering in sesame." BMC Plant Biology 18, no. 1: 343.