Fine root anatomy plays an important role in understanding the relationship between fine root function and soil environment. However, in different soil environments, the variation of fine root anatomical structure in different root sequences is not well studied. We measured the soil conditions and anatomical structure characteristics (root diameter, cortical tissue, vascular tissue and xylem) of fine roots of Cupressus funebris in four experimental sites, and analyzed each level of fine roots separately. We link these data to understand the relationship between fine root anatomy and soil conditions. We found that the anatomical structure of fine roots is closely related to soil environmental factors. The fine roots of lower root order are mainly affected by soil nutrients. Among them, the cortical tissue of first-order fine roots was positively correlated with potassium and phosphorus, but negatively correlated with nitrogen, while second- and third-order fine roots was positively correlated with soil total potassium and negatively correlated with nitrogen and phosphorus. For the fine roots of high root order, the cortical tissue disappeared, and the secondary vascular tissue was mainly affected by soil moisture. In addition, we also found that the division of fine root functional groups is not fixed. On the one hand, the function of third-order fine roots will slip. For example, the decrease of soil moisture will promote the transformation of third-order fine roots into transport roots, and the reduction of nitrogen will promote the transformation of third-order fine roots into absorption roots to fix nitrogen. This transformation strategy can effectively prevent the restriction of soil nutrients on plant growth. On the other hand, with the change of habitat, the first- and second-order fine roots are still the absorbing root, and the fourth- and fifth-order fine roots are still the transport root, but the efficiency of absorption and transport will be affected. In conclusion, our findings emphasize the fine roots in different soil environment to show high levels of plasticity, shows that fine root anatomical structure changes may make plants, and reveals that the fine is just order of reaction and its mechanism in the soil environment.

Pinus massoniana is an important tree species for wind protection and timber forests in Southern China. In recent years, P. massoniana plantations have been developed on more than 11,300,000 hm² in southern China, but numerous problems have been observed, such as soil degradation, biodiversity reduction, and ecological functional decline. Crop tree management impacts on fine root development, which can be explained by the variations in the root orders. In this study, a 36-year-old P. massoniana plantation located in Huaying, Sichuan Province, was selected as the research field. In 2015, crop tree management was initiated, with a crop tree intensity of 150 trees per hectare. After 3 years of growth, fine roots of crop and noncrop trees were collected by the sector method with an angle of 15 degrees and a radius of 2 meters. We analyzed the morphological characteristics and biomass in different root orders, and explored their carbon and nitrogen contents. The results were as follows: (1) The specific root length (SRL), root length density (RLD), and surface root area (SRA) of the crop trees were larger than those of the noncrop trees; the SRL increased significantly from 0–0.5 m to 1–1.5 m from the stem. (2) The fine root biomass of the crop trees was significantly larger than that of the noncrop trees. The fine root biomass of the crop and the noncrop trees increased with the horizontal distance from the stem from 0–0.5 m to 1–1.5 m. The morphological indexes of the noncrop trees at the distances of 1–1.5 m and 1.5–2 m were significantly different, while those of the crop trees at those distances were not. (3) The fine root C content of the crop trees was significantly higher than that of the noncrop trees and varied significantly along a vertical distribution. The fine root N content of the crop trees was significantly higher than that of the noncrop trees, and the N content of topsoil was higher than that of deeper soil. In conclusion, our results indicated that crop tree management increased the production of a large-diameter wood of P. massoniana, which might be attributed to the improvement of soil permeability and nutrient stock, and thus, the enhancement of fine root quantity and water/nutrient absorption ability.

Ectomycorrhizal (ECM) fungi can form symbioses with plant roots, which play an important role in regulating the rhizosphere microenvironment. As a broad-spectrum ECM tree species, Pinus massoniana forms symbiotic relationship called mycorrhiza with various ECM fungal species. In this study, four types of forests were selected from a 38-year-old Pinus plantation in eastern Sichuan, namely, pure P. massoniana forest (MC), P. massoniana mixed with Cunninghamia lanceolata forest (MS), P. massoniana–Cryptomeria fortunei forest (ML), and P. massoniana–broadleaved forest (MK), the species mixture ratio of all forests was 1:1. The ITS2 segment of ECM root tip sequenced by high-throughput sequencing using the Illumina MiSeq sequencing platform. (1) The ECM fungi of these four P. massoniana forests showed similar dominant genera but different relative abundances in community structure during the three seasons. (2) The alpha diversity index of ECM fungi was significantly influenced by season and forest type. (3) Soil pH, soil organic matter (SOM), total nitrogen (TN), C/N ratio, and total phosphorus (TP) influenced the ECM fungal community structure in different seasons. In summary, there were significant differences in ECM fungal communities among different forest types and different seasons; the colonization rate of ECM fungal in P. massoniana–Cunninghamia lanceolata was the highest, so we infer that Cunninghamia lanceolata is the most suitable tree species for mixed with P. massoniana in three mixture forests.

The upper reaches of the Yangtze River are a very important ecological barrier in China, but the ecological benefits of large-scale Cupressus funebris Endl.plantations are low. This study investigated 12 plantations of different compositions and densities, including two densities of Cinnamomum septentrionale Hand.-Mazz. (Cs), Alnus cremastogyne Burk. (Ac), and Toona sinensis (A. Juss.) Roem. (Ts), and mixed plantations of Cs + Ac (CA), Ts + Cs (TC), Ts + Ac (TA), and Ac + Ts + Cs (ATC) and the cutting-blank (CB), and, at the same time, the unreconstructed pure C. funebris (Cf) forest was set as the control. We aimed to explore the influence mechanism of upper tree composition and density on shrub diversity, as well as the relationship between shrub diversity and niche. Our research results are as follows: (1) Among all the patterns, the TA, CA, and TC patterns are the most conducive to improving the diversity of shrubs. The composition and density of different trees have a great influence on the diversity of shrubs. (2) Niche is closely related to the diversity of shrubs. In the patterns of low niche overlap between dominant shrubs, the diversity of shrubs is greater. These results contribute to a deeper understanding of the relationship between the diversity of overstory and shrubs, and reveals the relationship between niche and diversity.

The root system architecture (RSA), being a key characteristic of the root economic spectrum, describes the spatial arrangement and positioning of roots that determines the plant's exploration of water and nutrients in the soil. Still, it remains poorly understood how the RSA of woody plants responds to the demand for water and nutrients in different soil environments and how the uptake of these resources is optimized. Here we selected single-species plantations of Cupressus funebris and determined their topological index (TI), revised topological index (q_a and q_b), root link length (RLL), root branching rate (R_b and R_i:R_i+1), and in situ soil physicochemical properties to assess which root foraging strategies adopt in different soil environments among Guang'an City (GA), Suining City (SN), Mianyang City (MY), and Deyang City (DY) in China. We also tested the potential effects of different nutrients upon RSA according to its plastic phenotype. Principal component analysis (PCA) showed that levels of soil nutrients were the highest at DY, followed by MY and SN, and lower at GA. A dichotomous branching pattern was observed for GA, SN, and MY, but a herringbone branching pattern for DY. The RLL was ranked as GA, > SN, > MY > DY. The R_b of GA, SN, and MY was significantly lower than that of DY (p < 0.05). Among the different city regions, values of R₁/R₂ were the largest in different regions and those of R₄/R₅ the smallest. The cross-sectional area of the root system did not differ between any two connected branch orders. The TI, q_a, and RLL were significantly and negatively correlated with soil's water content, porosity, total nitrogen, total potassium, available nitrogen, and available phosphorus (p < 0.05), whereas they all had significant, positive relationships with soil temperature (p < 0.05). The R_b was significantly and positively correlated with total potassium in soil (p < 0.05). Redundancy analysis showed that total potassium was the main factor driving variation in RSA. Our results emphasize that the RSA is capable of corresponding plastic alterations by changing its number of internal or external links and the root link length of fine roots vis-à-vis a heterogeneous environment, thereby optimizing the rates of water capture and space utilization.

The natural regeneration of seedlings is a key factor for forest succession. Nevertheless, studies explaining the mechanism of growth and biomass allocation in regenerated seedlings after disturbance are lacking. Therefore, we measured the growth, biomass accumulation, and biomass allocation in current-age seedlings of Pinus massoniana after selective logging (logging of competitive trees, LCT; logging of inferior trees, LIT; and unlogged control, CK), and established structural equation models (SEMs) among the spatial structure characteristic indexes of the stand, environmental factors, and biomass allocation in different organs. As compared to the CK, the mingling index (M), uniform angle index (W), opening degree (O), soil organic carbon (SOC), available nitrogen (SAN), available phosphorus (SAP), available potassium (SAK), and bulk density (SBD) significantly increased (p < 0.05), while the competition index (CI) and neighborhood comparison (U) significantly decreased after logging (p < 0.05). After the LCT, seedling branch biomass improved, with an increase in the ground-diameter, crown-root ratio, and seedling quality index. More biomass was allocated to foliage and roots by an increase in the height and height-diameter ratio under the LIT. In the CK, increasing stem biomass helped the seedlings absorb and utilize more light. The Pearson correlation coefficient showed that biomass allocation to organs was independent, and seedlings adopted the strategies of heterogeneous adaptation and growth, thereby resulting in the separation of the allocation patterns among the organs. As per the redundancy analysis (RDA), CI was the main factor in biomass allocation. Environmental factors had direct effects on biomass allocation to organs, while the stand spatial structure characteristic indexes had indirect effects on biomass allocation based on SEMs. In summary, the LCT had significant, albeit indirect, effects on SOC, SAN, and SBD by reducing the CI for the regeneration and growth of seedlings in the stand, which was of great significance to the sustainable development of the forest stand of P. massoniana.