Pinus massoniana is an important tree species for wind protection and timber forests in Southern China. In recent years, P. massoniana plantations have been developed on more than 11,300,000 hm² in southern China, but numerous problems have been observed, such as soil degradation, biodiversity reduction, and ecological functional decline. Crop tree management impacts on fine root development, which can be explained by the variations in the root orders. In this study, a 36-year-old P. massoniana plantation located in Huaying, Sichuan Province, was selected as the research field. In 2015, crop tree management was initiated, with a crop tree intensity of 150 trees per hectare. After 3 years of growth, fine roots of crop and noncrop trees were collected by the sector method with an angle of 15 degrees and a radius of 2 meters. We analyzed the morphological characteristics and biomass in different root orders, and explored their carbon and nitrogen contents. The results were as follows: (1) The specific root length (SRL), root length density (RLD), and surface root area (SRA) of the crop trees were larger than those of the noncrop trees; the SRL increased significantly from 0–0.5 m to 1–1.5 m from the stem. (2) The fine root biomass of the crop trees was significantly larger than that of the noncrop trees. The fine root biomass of the crop and the noncrop trees increased with the horizontal distance from the stem from 0–0.5 m to 1–1.5 m. The morphological indexes of the noncrop trees at the distances of 1–1.5 m and 1.5–2 m were significantly different, while those of the crop trees at those distances were not. (3) The fine root C content of the crop trees was significantly higher than that of the noncrop trees and varied significantly along a vertical distribution. The fine root N content of the crop trees was significantly higher than that of the noncrop trees, and the N content of topsoil was higher than that of deeper soil. In conclusion, our results indicated that crop tree management increased the production of a large-diameter wood of P. massoniana, which might be attributed to the improvement of soil permeability and nutrient stock, and thus, the enhancement of fine root quantity and water/nutrient absorption ability.

Ectomycorrhizal (ECM) fungi can form symbioses with plant roots, which play an important role in regulating the rhizosphere microenvironment. As a broad-spectrum ECM tree species, Pinus massoniana forms symbiotic relationship called mycorrhiza with various ECM fungal species. In this study, four types of forests were selected from a 38-year-old Pinus plantation in eastern Sichuan, namely, pure P. massoniana forest (MC), P. massoniana mixed with Cunninghamia lanceolata forest (MS), P. massoniana–Cryptomeria fortunei forest (ML), and P. massoniana–broadleaved forest (MK), the species mixture ratio of all forests was 1:1. The ITS2 segment of ECM root tip sequenced by high-throughput sequencing using the Illumina MiSeq sequencing platform. (1) The ECM fungi of these four P. massoniana forests showed similar dominant genera but different relative abundances in community structure during the three seasons. (2) The alpha diversity index of ECM fungi was significantly influenced by season and forest type. (3) Soil pH, soil organic matter (SOM), total nitrogen (TN), C/N ratio, and total phosphorus (TP) influenced the ECM fungal community structure in different seasons. In summary, there were significant differences in ECM fungal communities among different forest types and different seasons; the colonization rate of ECM fungal in P. massoniana–Cunninghamia lanceolata was the highest, so we infer that Cunninghamia lanceolata is the most suitable tree species for mixed with P. massoniana in three mixture forests.

Rhizosphere microorganisms mediate the material exchange and chemical cycling between plant roots and soil. However, the response mechanisms of the rhizosphere microbial community, especially its co-occurrence patterns, to thinning remain poorly understood. We investigated the rhizosphere microbial communities of Pinus massoniana under different thinning intensities, including control (CK, 0%), light-intensity thinning (LIT, 10%), moderate-intensity thinning (MIT, 30%), and high-intensity thinning (HIT, 50%). Basic taxonomic information was obtained through high-throughput sequencing, while R software was utilized to identify thinning-sensitive operational taxonomic units (tsOTUs), construct co-occurrence networks, and perform other statistical analyses. Although no discernible patterns were observed in α-diversity changes, the Kruskal–Wallis test indicated that season was the primary factor driving α-diversity variation. Meanwhile, thinning intensity significantly shaped the rhizosphere microbial community structures, with each intensity harboring a specific tsOTUs subset. Although the top three modules of the meta-co-occurrence networks in summer and winter exhibited consistent tsOTU composition, winter triggered changes in network connectivity. Regardless of summer or winter, the number of network nodes under MIT was the highest. Additionally, after thinning, the relative abundances of most keystone taxa declined; however, MIT facilitated the enrichment of certain keystone taxa. Collectively, thinning profoundly shapes microbial community composition and network characteristics. Moderate thinning intensity may represent the optimal thinning intensity for the studied P. massoniana plantations.

Branch characteristics (quantity, morphology, and distribution) are critical determinants of tree growth and wood quality. However, the influence of species mixing, particularly mixing ratios, on branch development remains poorly understood. This study examined the branch attributes of Betula alnoides and Castanopsis hystrix in a six-year-old mixed-species trial plantation including monoculture of each species, and three mixtures at ratios of 1:1, 1:3, and 1:5 (B. alnoides–C. hystrix) in Pingxiang, Guangxi, China. Branch quantity (number, proportion, and density), morphology (diameter, length, and angle), and distribution (vertical and horizontal) were measured or recorded from 40 sampled dominant or codominant trees (20 B. alnoides and 20 C. hystrix). The results showed that mixing significantly increased the number and density of branches over 124.2% and 53.2%, respectively, in the lower crown (below 10 m) of B. alnoides, with these metrics positively correlated to the proportion of C. hystrix, while mixing exerted limited effects on branch quantity and size of C. hystrix. The 1:3 and 1:5 mixtures yielded more small branches (diameter < 10 mm) as well as more large branches (>25 mm) for B. alnoides. Branch distribution was almost uniform in different horizontal directions for both species, while variations in branch quantity and morphology along the stem were primarily species-specific; and both aspects remained consistent across the different mixing ratios. In conclusion, mixing B. alnoides with a low proportion of C. hystrix is proposed to produce high-quality solid wood for both species. Future studies should investigate alternative mixing patterns and higher proportions of B. alnoides in mixture with C. hystrix to optimize large-size and high-quality timber production.