This page has only limited features, please log in for full access.

Unclaimed
Kelly S. Bateman
International Centre of Excellence for Aquatic Animal Health (ICOE AAH), Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 26 August 2021 in Viruses
Reads 0
Downloads 0

Multiple enveloped viruses with rod-shaped nucleocapsids have been described, infecting the epithelial cell nuclei within the hepatopancreas tubules of crustaceans. These bacilliform viruses share the ultrastructural characteristics of nudiviruses, a specific clade of viruses infecting arthropods. Using histology, electron microscopy and high throughput sequencing, we characterise two further bacilliform viruses from aquatic hosts, the brown shrimp (Crangon crangon) and the European shore crab (Carcinus maenas). We assembled the full double stranded, circular DNA genome sequences of these viruses (~113 and 132 kbp, respectively). Comparative genomics and phylogenetic analyses confirm that both belong within the family Nudiviridae but in separate clades representing nudiviruses found in freshwater and marine environments. We show that the three thymidine kinase (tk) genes present in all sequenced nudivirus genomes, thus far, were absent in the Crangon crangon nudivirus, suggesting there are twenty-eight core genes shared by all nudiviruses. Furthermore, the phylogenetic data no longer support the subdivision of the family Nudiviridae into four genera (Alphanudivirus to Deltanudivirus), as recently adopted by the International Committee on Taxonomy of Viruses (ICTV), but rather shows two main branches of the family that are further subdivided. Our data support a recent proposal to create two subfamilies within the family Nudiviridae, each subdivided into several genera.

ACS Style

Kelly S. Bateman; Rose Kerr; Grant D. Stentiford; Tim P. Bean; Chantelle Hooper; Benigna Van Eynde; Daan Delbare; Jamie Bojko; Olivier Christiaens; Clauvis N. T. Taning; Guy Smagghe; Monique M. van Oers; Ronny van Aerle. Identification and Full Characterisation of Two Novel Crustacean Infecting Members of the Family Nudiviridae Provides Support for Two Subfamilies. Viruses 2021, 13, 1694 .

AMA Style

Kelly S. Bateman, Rose Kerr, Grant D. Stentiford, Tim P. Bean, Chantelle Hooper, Benigna Van Eynde, Daan Delbare, Jamie Bojko, Olivier Christiaens, Clauvis N. T. Taning, Guy Smagghe, Monique M. van Oers, Ronny van Aerle. Identification and Full Characterisation of Two Novel Crustacean Infecting Members of the Family Nudiviridae Provides Support for Two Subfamilies. Viruses. 2021; 13 (9):1694.

Chicago/Turabian Style

Kelly S. Bateman; Rose Kerr; Grant D. Stentiford; Tim P. Bean; Chantelle Hooper; Benigna Van Eynde; Daan Delbare; Jamie Bojko; Olivier Christiaens; Clauvis N. T. Taning; Guy Smagghe; Monique M. van Oers; Ronny van Aerle. 2021. "Identification and Full Characterisation of Two Novel Crustacean Infecting Members of the Family Nudiviridae Provides Support for Two Subfamilies." Viruses 13, no. 9: 1694.

Journal article
Published: 13 June 2021 in Viruses
Reads 0
Downloads 0

White Spot Disease (WSD) presents a major barrier to penaeid shrimp production. Mechanisms underlying White Spot Syndrome Virus (WSSV) susceptibility in penaeids are poorly understood due to limited information related to early infection. We investigated mRNA and miRNA transcription in Penaeus vannamei over 36 h following infection. Over this time course, 6192 transcripts and 27 miRNAs were differentially expressed—with limited differential expression from 3–12 h post injection (hpi) and a more significant transcriptional response associated with the onset of disease symptoms (24 hpi). During early infection, regulated processes included cytoskeletal remodelling and alterations in phagocytic activity that may assist WSSV entry and translocation, novel miRNA-induced metabolic shifts, and the downregulation of ATP-dependent proton transporter subunits that may impair cellular recycling. During later infection, uncoupling of the electron transport chain may drive cellular dysfunction and lead to high mortalities in infected penaeids. We propose that post-transcriptional silencing of the immune priming gene Dscam (downregulated following infections) by a novel shrimp miRNA (Pva-pmiR-78; upregulated) as a potential mechanism preventing future recognition of WSSV that may be suppressed in surviving shrimp. Our findings improve our understanding of WSD pathogenesis in P. vannamei and provide potential avenues for future development of prophylactics and treatments.

ACS Style

Rebecca Millard; Lisa Bickley; Kelly Bateman; Audrey Farbos; Diana Minardi; Karen Moore; Stuart Ross; Grant Stentiford; Charles Tyler; Ronny van Aerle; Eduarda Santos. Global mRNA and miRNA Analysis Reveal Key Processes in the Initial Response to Infection with WSSV in the Pacific Whiteleg Shrimp. Viruses 2021, 13, 1140 .

AMA Style

Rebecca Millard, Lisa Bickley, Kelly Bateman, Audrey Farbos, Diana Minardi, Karen Moore, Stuart Ross, Grant Stentiford, Charles Tyler, Ronny van Aerle, Eduarda Santos. Global mRNA and miRNA Analysis Reveal Key Processes in the Initial Response to Infection with WSSV in the Pacific Whiteleg Shrimp. Viruses. 2021; 13 (6):1140.

Chicago/Turabian Style

Rebecca Millard; Lisa Bickley; Kelly Bateman; Audrey Farbos; Diana Minardi; Karen Moore; Stuart Ross; Grant Stentiford; Charles Tyler; Ronny van Aerle; Eduarda Santos. 2021. "Global mRNA and miRNA Analysis Reveal Key Processes in the Initial Response to Infection with WSSV in the Pacific Whiteleg Shrimp." Viruses 13, no. 6: 1140.

Journal article
Published: 02 October 2020 in Viruses
Reads 0
Downloads 0

Mass mortalities of the larval stage of the giant freshwater prawn, Macrobrachium rosenbergii, have been occurring in Bangladesh since 2011. Mortalities can reach 100% and have resulted in an 80% decline in the number of hatcheries actively producing M. rosenbergii. To investigate a causative agent for the mortalities, a disease challenge was carried out using infected material from a hatchery experiencing mortalities. Moribund larvae from the challenge were prepared for metatranscriptomic sequencing. De novo virus assembly revealed a 29 kb single‑stranded positive-sense RNA virus with similarities in key protein motif sequences to yellow head virus (YHV), an RNA virus that causes mass mortalities in marine shrimp aquaculture, and other viruses in the Nidovirales order. Primers were designed against the novel virus and used to screen cDNA from larvae sampled from hatcheries in the South of Bangladesh from two consecutive years. Larvae from all hatcheries screened from both years were positive by PCR for the novel virus, including larvae from a hatchery that at the point of sampling appeared healthy, but later experienced mortalities. These screens suggest that the virus is widespread in M. rosenbergii hatchery culture in southern Bangladesh, and that early detection of the virus can be achieved by PCR. The hypothesised protein motifs of Macrobrachium rosenbergii golda virus (MrGV) suggest that it is likely to be a new species within the Nidovirales order. Biosecurity measures should be taken in order to mitigate global spread through the movement of post-larvae within and between countries, which has previously been linked to other virus outbreaks in crustacean aquaculture.

ACS Style

Chantelle Hooper; Partho Debnath; Sukumar Biswas; Ronny Van Aerle; Kelly Bateman; Siddhawartha Basak; Muhammad Rahman; Chadag Mohan; H. Islam; Stuart Ross; Grant Stentiford; David Currie; David Bass. A Novel RNA Virus, Macrobrachium rosenbergii Golda Virus (MrGV), Linked to Mass Mortalities of the Larval Giant Freshwater Prawn in Bangladesh. Viruses 2020, 12, 1120 .

AMA Style

Chantelle Hooper, Partho Debnath, Sukumar Biswas, Ronny Van Aerle, Kelly Bateman, Siddhawartha Basak, Muhammad Rahman, Chadag Mohan, H. Islam, Stuart Ross, Grant Stentiford, David Currie, David Bass. A Novel RNA Virus, Macrobrachium rosenbergii Golda Virus (MrGV), Linked to Mass Mortalities of the Larval Giant Freshwater Prawn in Bangladesh. Viruses. 2020; 12 (10):1120.

Chicago/Turabian Style

Chantelle Hooper; Partho Debnath; Sukumar Biswas; Ronny Van Aerle; Kelly Bateman; Siddhawartha Basak; Muhammad Rahman; Chadag Mohan; H. Islam; Stuart Ross; Grant Stentiford; David Currie; David Bass. 2020. "A Novel RNA Virus, Macrobrachium rosenbergii Golda Virus (MrGV), Linked to Mass Mortalities of the Larval Giant Freshwater Prawn in Bangladesh." Viruses 12, no. 10: 1120.

Journal article
Published: 16 June 2020 in Parasitology
Reads 0
Downloads 0

This study provides a morphological and phylogenetic characterization of two novel species of the order Haplosporida (Haplosporidium carcini n. sp., and H. cranc n. sp.) infecting the common shore crab Carcinus maenas collected at one location in Swansea Bay, South Wales, UK. Both parasites were observed in the haemolymph, gills and hepatopancreas. The prevalence of clinical infections (i.e. parasites seen directly in fresh haemolymph preparations) was low, at ~1%, whereas subclinical levels, detected by polymerase chain reaction, were slightly higher at ~2%. Although no spores were found in any of the infected crabs examined histologically (n = 334), the morphology of monokaryotic and dikaryotic unicellular stages of the parasites enabled differentiation between the two new species. Phylogenetic analyses of the new species based on the small subunit (SSU) rDNA gene placed H. cranc in a clade of otherwise uncharacterized environmental sequences from marine samples, and H. carcini in a clade with other crustacean-associated lineages.

ACS Style

Charlotte E. Davies; David Bass; Georgia M. Ward; Frederico M. Batista; Sophie H. Malkin; Jessica E. Thomas; Kelly Bateman; Stephen W. Feist; Christopher J. Coates; Andrew F. Rowley. Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp. Parasitology 2020, 147, 1 -9.

AMA Style

Charlotte E. Davies, David Bass, Georgia M. Ward, Frederico M. Batista, Sophie H. Malkin, Jessica E. Thomas, Kelly Bateman, Stephen W. Feist, Christopher J. Coates, Andrew F. Rowley. Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp. Parasitology. 2020; 147 (11):1-9.

Chicago/Turabian Style

Charlotte E. Davies; David Bass; Georgia M. Ward; Frederico M. Batista; Sophie H. Malkin; Jessica E. Thomas; Kelly Bateman; Stephen W. Feist; Christopher J. Coates; Andrew F. Rowley. 2020. "Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp." Parasitology 147, no. 11: 1-9.

Journal article
Published: 25 May 2020 in Viruses
Reads 0
Downloads 0

This is the first record of a fish nidovirus isolated from a consignment of goldfish at the United Kingdom (UK) border. The full-length viral genome was 25,985 nt, sharing a 97.9% nucleotide identity with the Chinook salmon bafinivirus (CSBV) NIDO with two deletions of 537 and 480 nt on the ORF Ia protein. To assess the potential impact on UK fish species, Atlantic salmon, common carp and goldfish were exposed to the virus via an intraperitoneal (IP) injection and bath challenge. Moribundity was recorded in only 8% of IP-injected goldfish. A high viral load, ≈107 of the CSBV PpIa gene, was measured in the kidney of moribund goldfish. Mild histopathological changes were observed in the kidneys of challenged carps. Ultrastructural observations in renal tubule epithelial cells of goldfish showed cylindrical tubes (≈15 nm in diameter) and tubular structures budding spherical virions (≈200 nm in diameter) with external spike-like structures. Negative staining showed both circular and bacilliform virions. Seroconversion was measured in common carp and goldfish but not in Atlantic salmon. This study reinforces the potential risk of novel and emerging pathogens being introduced to recipient countries via the international ornamental fish trade and the importance of regular full health screens at the border inspection posts to reduce this risk.

ACS Style

Irene Cano; David Stone; Jacqueline Savage; Gareth Wood; Brian Mulhearn; Joshua Gray; Nick Stinton; Stuart Ross; Michaela Bonar; Nick G. H. Taylor; Kelly S. Bateman; Stephen W. Feist. Isolation of a Chinook Salmon Bafinivirus (CSBV) in Imported Goldfish Carassius auratus L. in the United Kingdom and Evaluation of Its Virulence in Resident Fish Species. Viruses 2020, 12, 578 .

AMA Style

Irene Cano, David Stone, Jacqueline Savage, Gareth Wood, Brian Mulhearn, Joshua Gray, Nick Stinton, Stuart Ross, Michaela Bonar, Nick G. H. Taylor, Kelly S. Bateman, Stephen W. Feist. Isolation of a Chinook Salmon Bafinivirus (CSBV) in Imported Goldfish Carassius auratus L. in the United Kingdom and Evaluation of Its Virulence in Resident Fish Species. Viruses. 2020; 12 (5):578.

Chicago/Turabian Style

Irene Cano; David Stone; Jacqueline Savage; Gareth Wood; Brian Mulhearn; Joshua Gray; Nick Stinton; Stuart Ross; Michaela Bonar; Nick G. H. Taylor; Kelly S. Bateman; Stephen W. Feist. 2020. "Isolation of a Chinook Salmon Bafinivirus (CSBV) in Imported Goldfish Carassius auratus L. in the United Kingdom and Evaluation of Its Virulence in Resident Fish Species." Viruses 12, no. 5: 578.

Preprint content
Published: 12 May 2020
Reads 0
Downloads 0

1AbstractMass mortalities of the larval stage of the giant freshwater prawn, Macrobrachium rosenbergii, have been occurring in Bangladesh since 2011. Mortalities can reach 100% and have resulted in an 80% decline in the number of hatcheries actively producing M. rosenbergii. To investigate a causative agent for the mortalities, a disease challenge was carried out using infected material from a hatchery experiencing mortalities. Moribund larvae from the challenge were prepared for metatranscriptomic sequencing. De novo virus assembly revealed a 29 kb single-stranded positive-sense RNA virus with similarities in key protein motif sequences to yellow head virus (YHV), an RNA virus that causes mass mortalities in marine shrimp aquaculture, and other viruses in the Nidovirales order. Primers were designed against the novel virus and used to screen cDNA from larvae sampled from hatcheries in the South of Bangladesh from two consecutive years. Larvae from all hatcheries screened from both years were positive by PCR for the novel virus, including larvae from a hatchery that at the point of sampling appeared healthy, but later experienced mortalities. These screens suggest that the virus is widespread in M. rosenbergii hatchery culture in southern Bangladesh, and that early detection of the virus can be achieved by PCR. The hypothesised protein motifs of MrGV suggest that it is likely to be a new species within the Nidovirales order. Biosecurity measures should be taken in order to mitigate global spread through the movement of post-larvae within and between countries, which has previously been linked to other virus outbreaks in crustacean aquaculture.

ACS Style

Chantelle Hooper; Partho P. Debnath; Sukumar Biswas; Ronny Van Aerle; Kelly S. Bateman; Siddhawartha K. Basak; Muhammad M. Rahman; Chadag V. Mohan; H.M. Rakibul Islam; Stuart Ross; Grant D. Stentiford; David Currie; David Bass. A novel RNA virus, Macrobrachium rosenbergii Golda virus (MrGV), linked to mass mortalities of the larval giant freshwater prawn in Bangladesh. 2020, 1 .

AMA Style

Chantelle Hooper, Partho P. Debnath, Sukumar Biswas, Ronny Van Aerle, Kelly S. Bateman, Siddhawartha K. Basak, Muhammad M. Rahman, Chadag V. Mohan, H.M. Rakibul Islam, Stuart Ross, Grant D. Stentiford, David Currie, David Bass. A novel RNA virus, Macrobrachium rosenbergii Golda virus (MrGV), linked to mass mortalities of the larval giant freshwater prawn in Bangladesh. . 2020; ():1.

Chicago/Turabian Style

Chantelle Hooper; Partho P. Debnath; Sukumar Biswas; Ronny Van Aerle; Kelly S. Bateman; Siddhawartha K. Basak; Muhammad M. Rahman; Chadag V. Mohan; H.M. Rakibul Islam; Stuart Ross; Grant D. Stentiford; David Currie; David Bass. 2020. "A novel RNA virus, Macrobrachium rosenbergii Golda virus (MrGV), linked to mass mortalities of the larval giant freshwater prawn in Bangladesh." , no. : 1.

Journal article
Published: 17 November 2018 in Fish & Shellfish Immunology
Reads 0
Downloads 0

An in vitro model to study the host response to Neoparamoeba perurans, the causative agent of amoebic gill disease (AGD), was evaluated. The rainbow trout gill derived cell line, RTgill-W1, was seeded onto permeable cell culture supports and maintained asymmetrically with apical seawater. Cells were inoculated with either a passage attenuated or a recent wild clone of N. perurans. Amoebae, loaded with phagocytosed fluorescent beads, were observed associated with host cells within 20 min post inoculation (pi). By 6 h small foci of cytopathic effect appeared and at 72 h cytolysis was observed, with total disruption of the cell monolayer at 96 h pi. Due to cell monolayer disruption, the platform could not support proliferation of amoebae, which showed a 3-log reduction in parasite 18S rRNA mRNA after 72 h (106 copies at 1 h to 103 at 72 h pi). SEM observations showed amoebae-like cells with either short pseudopodia and a malleiform shape, or, long pseudopodia embedded within the gill cells and erosion of the cell monolayer. To study the host immune response, inoculated gill cells were harvested from triplicate inserts at 0, 1, 3, 6, 24 and 48 h pi, and expression of 12 genes involved in the Atlantic salmon response to AGD was compared between infected and uninfected cells and between amoebic clones. Both clones induced similar host inmate immune responses, with the up-regulation of proinflammatory cytokine IL1β, complement C3 and cell receptor MHC-1. The Th2 pathway was up-regulated, with increased gene expression of the transcription factor GATA3, and Th2 cytokines IL10, IL6 and IL4/13A. PCNA and AG-2 were also up-regulated. The wild clone induced significantly higher up-regulation of IL1β, MHC-1, PCNA, lysozyme and IL10 than the attenuated clone for at least some exposure times, but AG-2 gene expression was higher in cells inoculated with the attenuated one. A principal component analysis showed that AG-2 and IL10 were key genes in the in vitro host response to N. perurans. This in vitro model has proved to be a promising tool to study host responses to amoebae and may therefore reduce the requirement for in vivo studies when evaluating alternative therapeutants to AGD control.

ACS Style

Irene Cano; Nick Gh. Taylor; Amanda Bayley; Susie Gunning; Robin McCullough; Kelly Bateman; Barbara F. Nowak; Richard K. Paley. In vitro gill cell monolayer successfully reproduces in vivo Atlantic salmon host responses to Neoparamoeba perurans infection. Fish & Shellfish Immunology 2018, 86, 287 -300.

AMA Style

Irene Cano, Nick Gh. Taylor, Amanda Bayley, Susie Gunning, Robin McCullough, Kelly Bateman, Barbara F. Nowak, Richard K. Paley. In vitro gill cell monolayer successfully reproduces in vivo Atlantic salmon host responses to Neoparamoeba perurans infection. Fish & Shellfish Immunology. 2018; 86 ():287-300.

Chicago/Turabian Style

Irene Cano; Nick Gh. Taylor; Amanda Bayley; Susie Gunning; Robin McCullough; Kelly Bateman; Barbara F. Nowak; Richard K. Paley. 2018. "In vitro gill cell monolayer successfully reproduces in vivo Atlantic salmon host responses to Neoparamoeba perurans infection." Fish & Shellfish Immunology 86, no. : 287-300.

Applied studies
Published: 26 September 2018 in Journal of the World Aquaculture Society
Reads 0
Downloads 0

White spot disease in penaeid shrimp is caused by the white spot syndrome virus (WSSV). It is the most economically important disease of farmed warm‐water shrimp, causing extensive economic losses estimated from $8 to $15 billion since its emergence in the 1990s. Early diagnosis of disease is critical in the management of outbreaks and to avoid crop losses. Diagnosis of white spot disease is generally carried out in centralized laboratory settings using molecular biology approaches. However, this mode of testing can be expensive and time consuming, requiring laboratory equipment, highly trained laboratory personnel, dedicated laboratory space, and long‐distance transportation of samples from field to lab. In‐field diagnostics are gaining credence as tools for rapid and early animal disease detection, allowing diagnosticians and farmers to potentially manage disease outbreaks from the pond side. In the present study, we describe the development and application of a new in‐field point‐of‐need diagnostic test and platform for the diagnosis of WSSV in remote settings (shrimp farms). We report its performance in laboratory and field settings and compare it with current gold‐standard diagnostic approaches. We discuss the potential benefits (and barriers to uptake) of applying such testing in the global shrimp farming sector.

ACS Style

Diana Minardi; Kelly S. Bateman; Agnieszka Kuzdzal; Michelle Stone; Justin Avant; Richard Condliffe; Paul Brotherton; Mark Laverick; Kallaya Sritunyalucksana; Ornchuma Itsathitphaisarn; Puttharat Baoprasertkul; Grant D. Stentiford. Testing of a pond-side molecular diagnostic tool for the detection of white spot syndrome virus in shrimp aquaculture. Journal of the World Aquaculture Society 2018, 50, 18 -33.

AMA Style

Diana Minardi, Kelly S. Bateman, Agnieszka Kuzdzal, Michelle Stone, Justin Avant, Richard Condliffe, Paul Brotherton, Mark Laverick, Kallaya Sritunyalucksana, Ornchuma Itsathitphaisarn, Puttharat Baoprasertkul, Grant D. Stentiford. Testing of a pond-side molecular diagnostic tool for the detection of white spot syndrome virus in shrimp aquaculture. Journal of the World Aquaculture Society. 2018; 50 (1):18-33.

Chicago/Turabian Style

Diana Minardi; Kelly S. Bateman; Agnieszka Kuzdzal; Michelle Stone; Justin Avant; Richard Condliffe; Paul Brotherton; Mark Laverick; Kallaya Sritunyalucksana; Ornchuma Itsathitphaisarn; Puttharat Baoprasertkul; Grant D. Stentiford. 2018. "Testing of a pond-side molecular diagnostic tool for the detection of white spot syndrome virus in shrimp aquaculture." Journal of the World Aquaculture Society 50, no. 1: 18-33.

Journal article
Published: 14 March 2018 in Journal of Invertebrate Pathology
Reads 0
Downloads 0

Crangon crangon bacilliform virus (CcBV) was first discovered in 2004 in European brown shrimp (Crangon crangon) caught along the English coast. This study describes a duplex PCR assay developed for the detection of CcBV, based on amplification of the lef-8 gene (211 bp) of CcBV and the E75 gene (105 bp) of C. crangon as an internal amplification control. The lef-8 and E75 primer pairs were designed based on preliminary genome sequencing information of the virus and transcriptomic data available for C. crangon, respectively. Sequencing of the resulting amplicons confirmed the specificity of this PCR assay and sequence analysis of the lef-8 fragment revealed amino acid identity percentages ranging between 31 and 42% with members of the Nudiviridae, proposing that CcBV may reside within this family. Finally, the duplex PCR assay was applied to samples of C. crangon hepatopancreas tissue collected along the Belgian coast to screen for the presence of CcBV. The prevalence of CcBV averaged 87%, which is comparable to previous reports of high prevalence, based upon histological analysis, in shrimp collected along the English coast. Development of a specific and sensitive PCR assay to detect CcBV will provide a useful tool for future aquaculture and research programs involving C. crangon.

ACS Style

Benigna Van Eynde; Olivier Christiaens; Daan Delbare; Kris Cooreman; Kelly S. Bateman; Grant D. Stentiford; Annette Dullemans; Monique M. Van Oers; Guy Smagghe. Development and application of a duplex PCR assay for detection of Crangon crangon bacilliform virus in populations of European brown shrimp ( Crangon crangon ). Journal of Invertebrate Pathology 2018, 153, 195 -202.

AMA Style

Benigna Van Eynde, Olivier Christiaens, Daan Delbare, Kris Cooreman, Kelly S. Bateman, Grant D. Stentiford, Annette Dullemans, Monique M. Van Oers, Guy Smagghe. Development and application of a duplex PCR assay for detection of Crangon crangon bacilliform virus in populations of European brown shrimp ( Crangon crangon ). Journal of Invertebrate Pathology. 2018; 153 ():195-202.

Chicago/Turabian Style

Benigna Van Eynde; Olivier Christiaens; Daan Delbare; Kris Cooreman; Kelly S. Bateman; Grant D. Stentiford; Annette Dullemans; Monique M. Van Oers; Guy Smagghe. 2018. "Development and application of a duplex PCR assay for detection of Crangon crangon bacilliform virus in populations of European brown shrimp ( Crangon crangon )." Journal of Invertebrate Pathology 153, no. : 195-202.

Journal article
Published: 01 July 2017 in Journal of Invertebrate Pathology
Reads 0
Downloads 0
ACS Style

Monique M. Van Oers; Kelly S. Bateman; Grant D. Stentiford. Viruses of invertebrates related to the food-chain. Journal of Invertebrate Pathology 2017, 147, 1 -3.

AMA Style

Monique M. Van Oers, Kelly S. Bateman, Grant D. Stentiford. Viruses of invertebrates related to the food-chain. Journal of Invertebrate Pathology. 2017; 147 ():1-3.

Chicago/Turabian Style

Monique M. Van Oers; Kelly S. Bateman; Grant D. Stentiford. 2017. "Viruses of invertebrates related to the food-chain." Journal of Invertebrate Pathology 147, no. : 1-3.

Review
Published: 01 July 2017 in Journal of Invertebrate Pathology
Reads 0
Downloads 0

Numerous infections by viral pathogens have been described from wild and cultured crustacean hosts, yet relatively few of these pathogens have been formally characterised and classified. To date viruses have generally been tentatively assigned to families based upon morphological and developmental characteristics and their location of infection within the host cell. Often nucleotide sequence information is unavailable. Some of these viral infections have caused well-documented devastating consequences on the global crustacean farming industry whilst their effects on wild populations remain largely unstudied. This paper provides an up to date review of all known viruses described infecting crustacean hosts. Full characterisation and harmonisation of these descriptions utilising specifications proposed by the International Committee on Taxonomy of Viruses (ICTV) is required to synonymise numerous examples of differential naming or abbreviation of naming, of the same virus in some cases. Development and application of techniques such as viral purification and high throughput sequencing of viral genomes will assist with these full descriptions and, provide appropriate diagnostic targets for surveillance of known and novel relatives. This review also highlights the importance of comparative study with viruses infecting insects and other arthropods to assist this process.

ACS Style

K.S. Bateman; G.D. Stentiford. A taxonomic review of viruses infecting crustaceans with an emphasis on wild hosts. Journal of Invertebrate Pathology 2017, 147, 86 -110.

AMA Style

K.S. Bateman, G.D. Stentiford. A taxonomic review of viruses infecting crustaceans with an emphasis on wild hosts. Journal of Invertebrate Pathology. 2017; 147 ():86-110.

Chicago/Turabian Style

K.S. Bateman; G.D. Stentiford. 2017. "A taxonomic review of viruses infecting crustaceans with an emphasis on wild hosts." Journal of Invertebrate Pathology 147, no. : 86-110.

Journal article
Published: 12 April 2017 in Environmental Microbiology
Reads 0
Downloads 0

Glycolysis and oxidative phosphorylation are the fundamental pathways of ATP generation in eukaryotes. Yet in microsporidia, endoparasitic fungi living at the limits of cellular streamlining, oxidative phosphorylation has been lost: energy is obtained directly from the host or, during the dispersive spore stage, via glycolysis. It was therefore surprising when the first sequenced genome from the Enterocytozoonidae – a major family of human and animal-infecting microsporidians – appeared to have lost genes for glycolysis. Here, we sequence and analyse genomes from additional members of this family, shedding new light on their unusual biology. Our survey includes the genome of Enterocytozoon hepatopenaei, a major aquacultural parasite currently causing substantial economic losses in shrimp farming, and Enterospora canceri, a pathogen that lives exclusively inside epithelial cell nuclei of its crab host. Our analysis of gene content across the clade suggests that Ent. canceri's adaptation to intranuclear life is underpinned by the expansion of transporter families. We demonstrate that this entire lineage of pathogens has lost glycolysis and, uniquely amongst eukaryotes, lacks any obvious intrinsic means of generating energy. Our study provides an important resource for the investigation of host-pathogen interactions and reductive evolution in one of the most medically and economically important microsporidian lineages.

ACS Style

Dominic Wiredu Boakye; Pattana Jaroenlak; Anuphap Prachumwat; Tom Williams; Kelly S. Bateman; Ornchuma Itsathitphaisarn; Kallaya Sritunyalucksana; Konrad H. Paszkiewicz; Karen A. Moore; Grant D. Stentiford; Bryony Williams. Decay of the glycolytic pathway and adaptation to intranuclear parasitism within Enterocytozoonidae microsporidia. Environmental Microbiology 2017, 19, 2077 -2089.

AMA Style

Dominic Wiredu Boakye, Pattana Jaroenlak, Anuphap Prachumwat, Tom Williams, Kelly S. Bateman, Ornchuma Itsathitphaisarn, Kallaya Sritunyalucksana, Konrad H. Paszkiewicz, Karen A. Moore, Grant D. Stentiford, Bryony Williams. Decay of the glycolytic pathway and adaptation to intranuclear parasitism within Enterocytozoonidae microsporidia. Environmental Microbiology. 2017; 19 (5):2077-2089.

Chicago/Turabian Style

Dominic Wiredu Boakye; Pattana Jaroenlak; Anuphap Prachumwat; Tom Williams; Kelly S. Bateman; Ornchuma Itsathitphaisarn; Kallaya Sritunyalucksana; Konrad H. Paszkiewicz; Karen A. Moore; Grant D. Stentiford; Bryony Williams. 2017. "Decay of the glycolytic pathway and adaptation to intranuclear parasitism within Enterocytozoonidae microsporidia." Environmental Microbiology 19, no. 5: 2077-2089.

Review
Published: 01 September 2016 in International Journal for Parasitology
Reads 0
Downloads 0

Paramyxida is an order of rhizarian protists that parasitise marine molluscs, annelids and crustaceans. They include notifiable pathogens (Marteilia spp.) of bivalves and other taxa of economic significance for shellfish production. The diversity of paramyxids is poorly known, particularly outside of commercially important hosts, and their phylogenetic position is unclear due to their extremely divergent 18S rDNA sequences. However, novel paramyxean lineages are increasingly being detected in a wide range of invertebrate hosts, and interest in the group is growing, marked by the first ‘Paramyxean Working Group’ Meeting held in Spain in February 2015. We review the diversity, host affiliations, and geographical ranges of all known paramyxids, present a comprehensive phylogeny of the order and clarify its taxonomy. Our phylogenetic analyses confirm the separate status of four genera: Paramarteilia, Marteilioides, Paramyxa and Marteilia. Further, as including M. granula in Marteilia would make the genus paraphyletic we suggest transferring this species to a new genus, Eomarteilia. We present sequence data for Paramyxa nephtys comb. n., a parasite of polychaete worms, providing morphological data for a clade of otherwise environmental sequences, sister to Paramarteilia. Light and electron microscopy analyses show strong similarities with both Paramyxa and Paramyxoides, and we further discuss the validity of those two genera. We provide histological and electron microscopic data for Paramarteilia orchestiae, the type species of that genus originally described from the amphipod Orchestia; in situ hybridisation shows that Paramarteilia also infects crab species. We present, to our knowledge, the first known results of a paramyxid-specific environmental DNA survey of environmental (filtered water, sediment, etc.) and organismally-derived samples, revealing new lineages and showing that paramyxids are associated with a wider range of hosts and habitat types than previously known. On the basis of our new phylogeny we propose phylogenetic hypotheses for evolution of lifecycle and infectivity traits observed in different paramyxid genera.

ACS Style

Georgia M. Ward; Martyn Bennett; Kelly Bateman; Grant D. Stentiford; Rose Kerr; Stephen W. Feist; Suzanne Williams; Cédric Berney; David Bass. A new phylogeny and environmental DNA insight into paramyxids: an increasingly important but enigmatic clade of protistan parasites of marine invertebrates. International Journal for Parasitology 2016, 46, 605 -619.

AMA Style

Georgia M. Ward, Martyn Bennett, Kelly Bateman, Grant D. Stentiford, Rose Kerr, Stephen W. Feist, Suzanne Williams, Cédric Berney, David Bass. A new phylogeny and environmental DNA insight into paramyxids: an increasingly important but enigmatic clade of protistan parasites of marine invertebrates. International Journal for Parasitology. 2016; 46 (10):605-619.

Chicago/Turabian Style

Georgia M. Ward; Martyn Bennett; Kelly Bateman; Grant D. Stentiford; Rose Kerr; Stephen W. Feist; Suzanne Williams; Cédric Berney; David Bass. 2016. "A new phylogeny and environmental DNA insight into paramyxids: an increasingly important but enigmatic clade of protistan parasites of marine invertebrates." International Journal for Parasitology 46, no. 10: 605-619.

Review article
Published: 19 June 2016 in Journal of Sea Research
Reads 0
Downloads 0

Diseases in marine invertebrates are increasing in both frequency and intensity around the globe. Diseases in individuals which offer some commercial value are often well documented and subsequently well studied in comparison to those wild groups offering little commercial gain. This is particularly the case with those associated with mariculture or the commercial fisheries. Specifically, these include many Holothuroidea, and numerous crustacea and mollusca species. Pathogens/parasites consisting of both prokaryotes and eukaryotes from all groups have been associated with diseases from such organisms, including bacteria, viruses, fungi and protozoa. Viral pathogens in particular, appear to be an increasingly important group and research into this group will likely highlight a larger number of diseases and pathogens being described in the near future. Interestingly, although there are countless examples of the spread of disease usually associated with transportation of specific infected hosts for development of aquaculture practices, this process appears to be continuing with no real sign of effective management and mitigation strategies being implicated. Notably, even in well developed countries such as the UK and the US, even though live animal trade may be well managed, the transport of frozen food appears to be less well so and as evidence suggests, even these to have the potential to transmit pathogens when used as a food source for example.

ACS Style

Michael J. Sweet; Kelly S. Bateman. Reprint of ‘Diseases in marine invertebrates associated with mariculture and commercial fisheries’. Journal of Sea Research 2016, 113, 28 -44.

AMA Style

Michael J. Sweet, Kelly S. Bateman. Reprint of ‘Diseases in marine invertebrates associated with mariculture and commercial fisheries’. Journal of Sea Research. 2016; 113 ():28-44.

Chicago/Turabian Style

Michael J. Sweet; Kelly S. Bateman. 2016. "Reprint of ‘Diseases in marine invertebrates associated with mariculture and commercial fisheries’." Journal of Sea Research 113, no. : 28-44.

Journal article
Published: 22 March 2016 in Parasitology
Reads 0
Downloads 0

SUMMARY Almost half of all known microsporidian taxa infect aquatic animals. Of these, many cause disease in arthropods.Hepatospora, a recently erected genus, infects epithelial cells of the hepatopancreas of wild and farmed decapod crustaceans. We isolatedHepatosporaspp. from three different crustacean hosts, inhabiting different habitats and niches; marine edible crab (Cancer pagurus), estuarine and freshwater Chinese mitten crab (Eriocheir sinensis) and the marine mussel symbiont pea crab (Pinnotheres pisum). Isolates were initially compared using histology and electron microscopy revealing variation in size, polar filament arrangement and nuclear development. However, sequence analysis of the partial SSU rDNA gene could not distinguish between the isolates (~99% similarity). In an attempt to resolve the relationship betweenHepatosporaisolated fromE. sinensisandC. pagurus, six additional gene sequences were mined from on-going unpublished genome projects (RNA polymerase, arginyl tRNA synthetase, prolyl tRNA synthetase, chitin synthase, beta tubulin and heat shock protein 70). Primers were designed based on the above gene sequences to analyseHepatosporaisolated from pea crab. Despite application of gene sequences to concatenated phylogenies, we were unable to discriminateHepatosporaisolates obtained from these hosts and concluded that they likely represent a single species or, at least subspecies thereof. In this instance, concatenated phylogenetic analysis supported the SSU-based phylogeny, and further, demonstrated that microsporidian taxonomies based upon morphology alone are unreliable, even at the level of the species. Our data, together with description ofH. eriocheirin Asian crab farms, reveal a preponderance for microvariants of this parasite to infect the gut of a wide array of decapods crustacean hosts and the potential forHepatosporato exist as a cline across wide geographies and habitats.

ACS Style

K. S. Bateman; Dominic Wiredu-Boakye; R. Kerr; Bryony Williams; G. D. Stentiford. Single and multi-gene phylogeny ofHepatospora(Microsporidia) – a generalist pathogen of farmed and wild crustacean hosts. Parasitology 2016, 143, 971 -982.

AMA Style

K. S. Bateman, Dominic Wiredu-Boakye, R. Kerr, Bryony Williams, G. D. Stentiford. Single and multi-gene phylogeny ofHepatospora(Microsporidia) – a generalist pathogen of farmed and wild crustacean hosts. Parasitology. 2016; 143 (8):971-982.

Chicago/Turabian Style

K. S. Bateman; Dominic Wiredu-Boakye; R. Kerr; Bryony Williams; G. D. Stentiford. 2016. "Single and multi-gene phylogeny ofHepatospora(Microsporidia) – a generalist pathogen of farmed and wild crustacean hosts." Parasitology 143, no. 8: 971-982.

Review
Published: 18 January 2016 in Viruses
Reads 0
Downloads 0

Since its emergence in the 1990s, White Spot Disease (WSD) has had major economic and societal impact in the crustacean aquaculture sector. Over the years shrimp farming alone has experienced billion dollar losses through WSD. The disease is caused by the White Spot Syndrome Virus (WSSV), a large dsDNA virus and the only member of the Nimaviridae family. Susceptibility to WSSV in a wide range of crustacean hosts makes it a major risk factor in the translocation of live animals and in commodity products. Currently there are no effective treatments for this disease. Understanding the molecular basis of disease processes has contributed significantly to the treatment of many human and animal pathogens, and with a similar aim considerable efforts have been directed towards understanding host–pathogen molecular interactions for WSD. Work on the molecular mechanisms of pathogenesis in aquatic crustaceans has been restricted by a lack of sequenced and annotated genomes for host species. Nevertheless, some of the key host–pathogen interactions have been established: between viral envelope proteins and host cell receptors at initiation of infection, involvement of various immune system pathways in response to WSSV, and the roles of various host and virus miRNAs in mitigation or progression of disease. Despite these advances, many fundamental knowledge gaps remain; for example, the roles of the majority of WSSV proteins are still unknown. In this review we assess current knowledge of how WSSV infects and replicates in its host, and critique strategies for WSD treatment.

ACS Style

Bas Verbruggen; Lisa K. Bickley; Ronny Van Aerle; Kelly S. Bateman; Grant D. Stentiford; Eduarda M. Santos; Charles R. Tyler. Molecular Mechanisms of White Spot Syndrome Virus Infection and Perspectives on Treatments. Viruses 2016, 8, 23 .

AMA Style

Bas Verbruggen, Lisa K. Bickley, Ronny Van Aerle, Kelly S. Bateman, Grant D. Stentiford, Eduarda M. Santos, Charles R. Tyler. Molecular Mechanisms of White Spot Syndrome Virus Infection and Perspectives on Treatments. Viruses. 2016; 8 (1):23.

Chicago/Turabian Style

Bas Verbruggen; Lisa K. Bickley; Ronny Van Aerle; Kelly S. Bateman; Grant D. Stentiford; Eduarda M. Santos; Charles R. Tyler. 2016. "Molecular Mechanisms of White Spot Syndrome Virus Infection and Perspectives on Treatments." Viruses 8, no. 1: 23.

Journal article
Published: 03 July 2015 in Journal of Invertebrate Pathology
Reads 0
Downloads 0

This paper utilises histological, ultrastructure and molecular phylogenetic data to describe a novel genus and species (Paradoxium irvingi n.gen., n.sp.) within clade 5 of the phylum Microsporidia. The parasite infects the musculature of the pink shrimp Pandalus montagui captured from United Kingdom waters. The novel microsporidium is morphologically and phylogenetically dissimilar to its nearest phylogenetic branch relative Thelohania butleri infecting the sister shrimp taxon Pandalus jordani. Furthermore, it is morphologically distinct from the type species of the genus Thelohania, Thelohania giardi infecting European brown shrimp Crangon crangon. Since phylogenetic data pertaining to type T. giardi is not currently available, our discovery places some doubt on the likelihood that T. butleri represents the proposed surrogate for the type taxon. Further it demonstrates potential for significant morphological plasticity in this clade of muscle-infecting microsporidians of crustaceans which contains the genera Myospora, Cucumispora, Thelohania, and now Paradoxium. Since it cannot be stated with certainty that T. butleri (or other taxa within the clade) represent true close relatives of T. giardi, clarity on this issue will only occur with re-discovery and genotyping of type T. giardi infecting C. crangon from European waters.

ACS Style

G.D. Stentiford; S.H. Ross; R. Kerr; D. Bass; Kelly Bateman. Paradoxium irvingi n.gen. n.sp. (Microsporidia) infecting the musculature of European pink shrimp Pandalus montagui. Journal of Invertebrate Pathology 2015, 130, 1 -8.

AMA Style

G.D. Stentiford, S.H. Ross, R. Kerr, D. Bass, Kelly Bateman. Paradoxium irvingi n.gen. n.sp. (Microsporidia) infecting the musculature of European pink shrimp Pandalus montagui. Journal of Invertebrate Pathology. 2015; 130 ():1-8.

Chicago/Turabian Style

G.D. Stentiford; S.H. Ross; R. Kerr; D. Bass; Kelly Bateman. 2015. "Paradoxium irvingi n.gen. n.sp. (Microsporidia) infecting the musculature of European pink shrimp Pandalus montagui." Journal of Invertebrate Pathology 130, no. : 1-8.

Review article
Published: 02 July 2015 in Journal of Sea Research
Reads 0
Downloads 0

Diseases in marine invertebrates are increasing in both frequency and intensity around the globe. Diseases in individuals which offer some commercial value are often well documented and subsequently well studied in comparison to those wild groups offering little commercial gain. This is particularly the case with those associated with mariculture or the commercial fisheries. Specifically, these include many Holothuroidea, and numerous crustacea and mollusca species. Pathogens/parasites consisting of both prokaryotes and eukaryotes from all groups have been associated with diseases from such organisms, including bacteria, viruses, fungi and protozoa. Viral pathogens in particular, appear to be an increasingly important group and research into this group will likely highlight a larger number of diseases and pathogens being described in the near future. Interestingly, although there are countless examples of the spread of disease usually associated with transportation of specific infected hosts for development of aquaculture practices, this process appears to be continuing with no real sign of effective management and mitigation strategies being implicated. Notably, even in well developed countries such as the UK and the US, even though live animal trade may be well managed, the transport of frozen food appears to be less well so and as evidence suggests, even these to have the potential to transmit pathogens when used as a food source for example.

ACS Style

Michael J. Sweet; Kelly Bateman. Diseases in marine invertebrates associated with mariculture and commercial fisheries. Journal of Sea Research 2015, 104, 16 -32.

AMA Style

Michael J. Sweet, Kelly Bateman. Diseases in marine invertebrates associated with mariculture and commercial fisheries. Journal of Sea Research. 2015; 104 ():16-32.

Chicago/Turabian Style

Michael J. Sweet; Kelly Bateman. 2015. "Diseases in marine invertebrates associated with mariculture and commercial fisheries." Journal of Sea Research 104, no. : 16-32.

Journal article
Published: 16 June 2015 in BMC Genomics
Reads 0
Downloads 0

The European shore crab, Carcinus maenas, is used widely in biomonitoring, ecotoxicology and for studies into host-pathogen interactions. It is also an important invasive species in numerous global locations. However, the genomic resources for this organism are still sparse, limiting research progress in these fields. To address this resource shortfall we produced a C. maenas transcriptome, enabled by the progress in next-generation sequencing technologies, and applied this to assemble information on the innate immune system in this species. We isolated and pooled RNA for twelve different tissues and organs from C. maenas individuals and sequenced the RNA using next generation sequencing on an Illumina HiSeq 2500 platform. After de novo assembly a transcriptome was generated encompassing 212,427 transcripts (153,699 loci). The transcripts were filtered, annotated and characterised using a variety of tools (including BLAST, MEGAN and RSEM) and databases (including NCBI, Gene Ontology and KEGG). There were differential patterns of expression for between 1,223 and 2,741 transcripts across tissues and organs with over-represented Gene Ontology terms relating to their specific function. Based on sequence homology to immune system components in other organisms, we show both the presence of transcripts for a series of known pathogen recognition receptors and response proteins that form part of the innate immune system, and transcripts representing the RNAi, Toll-like receptor signalling, IMD and JAK/STAT pathways. We have produced an assembled transcriptome for C. maenas that provides a significant molecular resource for wide ranging studies in this species. Analysis of the transcriptome has revealed the presence of a series of known targets and functional pathways that form part of their innate immune system and illustrate tissue specific differences in their expression patterns. The online version of this article (doi:10.1186/s12864-015-1667-1) contains supplementary material, which is available to authorized users.

ACS Style

Bas Verbruggen; Lisa K. Bickley; Eduarda M. Santos; Charles R. Tyler; Grant D. Stentiford; Kelly S. Bateman; Ronny Van Aerle. De novo assembly of the Carcinus maenas transcriptome and characterization of innate immune system pathways. BMC Genomics 2015, 16, 458 .

AMA Style

Bas Verbruggen, Lisa K. Bickley, Eduarda M. Santos, Charles R. Tyler, Grant D. Stentiford, Kelly S. Bateman, Ronny Van Aerle. De novo assembly of the Carcinus maenas transcriptome and characterization of innate immune system pathways. BMC Genomics. 2015; 16 (1):458.

Chicago/Turabian Style

Bas Verbruggen; Lisa K. Bickley; Eduarda M. Santos; Charles R. Tyler; Grant D. Stentiford; Kelly S. Bateman; Ronny Van Aerle. 2015. "De novo assembly of the Carcinus maenas transcriptome and characterization of innate immune system pathways." BMC Genomics 16, no. 1: 458.

Journal article
Published: 09 October 2014 in Journal of Fish Diseases
Reads 0
Downloads 0

A challenge model for pancreas disease in Atlantic salmon, Salmo salar L. fry, was developed comparing two salmonid alphavirus (SAV) subtypes: SAV1 and SAV5. Viral doses of 3 × 10(5) TCID50 mL(-1) for SAV1 and 3 × 10(4) for SAV5 were tested in triplicate tanks, each containing 450 salmon fry. Cumulative mortalities of 1.2% were recorded. Titres of virus recovered from the mortalities ranged from 10(2) to 10(7) TCID50 mL(-1) . Fry were sampled at 3, 5 and 7.5 weeks post-challenge. Sampling after 3 weeks revealed a high prevalence of infection in the absence of clinical signs, and infectious virus was recovered from 80% and 43% of sampled fry infected with SAV1 and SAV5, respectively. After 5 weeks pancreas, heart and red skeletal muscle lesions were generally observed, whilst degeneration in white skeletal muscle was observed only in fish infected with SAV1. In situ hybridisation confirmed the presence of viral genome in infected pancreas, heart and muscle. After 7.5 weeks, infectious virus (both isolates) was recovered from 13.3% of the fish sampled, with a viral titre of 10(2) TCID50 mL(-1) . Clearly, salmon fry are susceptible to SAV infection and pancreas disease.

ACS Style

I Cano; C Joiner; A Bayley; G Rimmer; Kelly Bateman; S W Feist; D Stone; R Paley. An experimental means of transmitting pancreas disease in Atlantic salmon Salmo salar L. fry in freshwater. Journal of Fish Diseases 2014, 38, 271 -281.

AMA Style

I Cano, C Joiner, A Bayley, G Rimmer, Kelly Bateman, S W Feist, D Stone, R Paley. An experimental means of transmitting pancreas disease in Atlantic salmon Salmo salar L. fry in freshwater. Journal of Fish Diseases. 2014; 38 (3):271-281.

Chicago/Turabian Style

I Cano; C Joiner; A Bayley; G Rimmer; Kelly Bateman; S W Feist; D Stone; R Paley. 2014. "An experimental means of transmitting pancreas disease in Atlantic salmon Salmo salar L. fry in freshwater." Journal of Fish Diseases 38, no. 3: 271-281.