Introduction: The rapid advancement of artificial intelligence (AI) is fundamentally transforming educational and employment landscapes, generating increasing psychological concerns among students in technology-intensive fields. This study examines AI-related anxiety, digital well-being, and career uncertainty among engineering and information technology (IT) students, with a focus on their prevalence, interrelationships, and demographic variations. Methods: A cross-sectional quantitative design was employed using a structured survey administered to 820 undergraduate students from four Jordanian universities. Standardized measures were used to assess AI anxiety, digital well-being, and career-related concerns. Statistical analyses included descriptive statistics, correlation analysis, and group comparisons based on gender and academic discipline. Results: The findings indicated elevated levels of AI anxiety ( M = 5.26, SD = 0.32), low levels of digital well-being ( M = 1.75, SD = 0.20), and moderate levels of career concerns ( M = 4.07, SD = 0.34). AI anxiety was strongly negatively correlated with digital well-being ( r = −0.849, p < 0.01) and positively correlated with career concerns ( r = 0.680, p < 0.01). Female students reported significantly higher AI anxiety and career concerns than male students ( p < 0.001). Additionally, IT students exhibited higher levels of AI anxiety and career uncertainty compared to engineering students ( p < 0.001). Discussion: These findings highlight the psychological impact of AI integration on students, emphasizing the need for targeted AI literacy programs, digital well-being interventions, and career guidance strategies. Addressing gender disparities and discipline-specific differences is essential to enhance students’ resilience, adaptability, and readiness for an AI-driven labor market.