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Dr. Mitsutaka Fukudome
Division of Symbiotic Systems, National Institute for Basic Biology

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Research Keywords & Expertise

0 Nitric Oxide
0 reactive sulfur species
0 Lotus japonicus
0 stress response and tolerance
0 Root nodule symbiosis

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Short Biography

Division of Symbiotic Systems, National Institute for Basic Biology, Myodaiji, Okazaki, Aichi 444-8585, Japan

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Journal article
Published: 17 February 2021 in Plants
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Under water deficit conditions, the essential macronutrient nitrogen becomes limited as a result of reduced dissolved nitrogen and root nitrogen uptake. An elevated nitrogen level might be able to mitigate these effects, integrated with the idea of using nitric oxide as abiotic stress tolerant inducers. In this study, we evaluated the potential of using elevated nitrogen priming prior to water shortage to mitigate plant stress through nitric oxide accumulation. We grew rice plants in 300 mg L−1 nitrogen for 10 weeks, then we primed plants with four different nitrogen concentrations: 100, 300 (control), 500 and 1000 mg L−1 nitrogen prior to inducing water deficit conditions. Plants primed with 500 mg L−1 nitrogen possessed a higher photosynthetic rate, relative water content, electrolyte leakage and lipid peroxidation under water deficit conditions, compared to control plants. The induction of water deficit tolerance was supported with the activation of antioxidant defense system, induced by the accumulation of nitric oxide in leaves and roots of rice plants. We originally demonstrated the accumulation of nitric oxide in leaves of rice plants. The elevated nitrogen priming can be used to enhance water deficit tolerance in irrigated paddy fields, instead of nitric oxide donors.

ACS Style

Kamolchanok Umnajkitikorn; Mitsutaka Fukudome; Toshiki Uchiumi; Neung Teaumroong. Elevated Nitrogen Priming Induced Oxinitro-Responses and Water Deficit Tolerance in Rice. Plants 2021, 10, 381 .

AMA Style

Kamolchanok Umnajkitikorn, Mitsutaka Fukudome, Toshiki Uchiumi, Neung Teaumroong. Elevated Nitrogen Priming Induced Oxinitro-Responses and Water Deficit Tolerance in Rice. Plants. 2021; 10 (2):381.

Chicago/Turabian Style

Kamolchanok Umnajkitikorn; Mitsutaka Fukudome; Toshiki Uchiumi; Neung Teaumroong. 2021. "Elevated Nitrogen Priming Induced Oxinitro-Responses and Water Deficit Tolerance in Rice." Plants 10, no. 2: 381.

Journal article
Published: 07 February 2020 in Antioxidants
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Reactive sulfur species (RSS) function as strong antioxidants and are involved in various biological responses in animals and bacteria. Few studies; however, have examined RSS in plants. In the present study, we clarified that RSS are involved in root nodule symbiosis in the model legume Lotus japonicus. Polysulfides, a type of RSS, were detected in the roots by using a sulfane sulfur-specific fluorescent probe, SSP4. Supplying the sulfane sulfur donor Na2S3 to the roots increased the amounts of both polysulfides and hydrogen sulfide (H2S) in the roots and simultaneously decreased the amounts of nitric oxide (NO) and reactive oxygen species (ROS). RSS were also detected in infection threads in the root hairs and in infected cells of nodules. Supplying the sulfane sulfur donor significantly increased the numbers of infection threads and nodules. When nodules were immersed in the sulfane sulfur donor, their nitrogenase activity was significantly reduced, without significant changes in the amounts of NO, ROS, and H2S. These results suggest that polysulfides interact with signal molecules such as NO, ROS, and H2S in root nodule symbiosis in L. japonicus. SSP4 and Na2S3 are useful tools for study of RSS in plants.

ACS Style

Mitsutaka Fukudome; Hazuki Shimada; Nahoko Uchi; Ken-Ichi Osuki; Haruka Ishizaki; Ei-Ichi Murakami; Masayoshi Kawaguchi; Toshiki Uchiumi. Reactive Sulfur Species Interact with Other Signal Molecules in Root Nodule Symbiosis in Lotus japonicus. Antioxidants 2020, 9, 145 .

AMA Style

Mitsutaka Fukudome, Hazuki Shimada, Nahoko Uchi, Ken-Ichi Osuki, Haruka Ishizaki, Ei-Ichi Murakami, Masayoshi Kawaguchi, Toshiki Uchiumi. Reactive Sulfur Species Interact with Other Signal Molecules in Root Nodule Symbiosis in Lotus japonicus. Antioxidants. 2020; 9 (2):145.

Chicago/Turabian Style

Mitsutaka Fukudome; Hazuki Shimada; Nahoko Uchi; Ken-Ichi Osuki; Haruka Ishizaki; Ei-Ichi Murakami; Masayoshi Kawaguchi; Toshiki Uchiumi. 2020. "Reactive Sulfur Species Interact with Other Signal Molecules in Root Nodule Symbiosis in Lotus japonicus." Antioxidants 9, no. 2: 145.

Journal article
Published: 01 January 2020 in Microbes and Environments
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Bradyrhizobium sp. strain SUTN9-2 is a symbiotic and endophytic diazotrophic bacterium found in legume and rice plants and has the potential to promote growth. The present results revealed that SUTN9-2 underwent cell enlargement, increased its DNA content, and efficiently performed nitrogen fixation in response to rice extract. Some factors in rice extract induced the expression of cell cycle and nitrogen fixation genes. According to differentially expressed genes (DEGs) from the transcriptomic analysis, SUTN9-2 was affected by rice extract and the deletion of the bclA gene. The up-regulated DEGs encoding a class of oxidoreductases, which act with oxygen atoms and may have a role in controlling oxygen at an appropriate level for nitrogenase activity, followed by GroESL chaperonins are required for the function of nitrogenase. These results indicate that following its exposure to rice extract, nitrogen fixation by SUTN9-2 is induced by the collective effects of GroESL and oxidoreductases. The expression of the sensitivity to antimicrobial peptides transporter (sapDF) was also up-regulated, resulting in cell differentiation, even when bclA (sapDF) was mutated. This result implies similarities in the production of defensin-like antimicrobial peptides (DEFs) by rice and nodule-specific cysteine-rich (NCR) peptides in legume plants, which affect bacterial cell differentiation.

ACS Style

Teerana Greetatorn; Shun Hashimoto; Taro Maeda; Mitsutaka Fukudome; Pongdet Piromyou; Kamonluck Teamtisong; Panlada Tittabutr; Nantakorn Boonkerd; Masayoshi Kawaguchi; Toshiki Uchiumi; Neung Teaumroong. Mechanisms of Rice Endophytic Bradyrhizobial Cell Differentiation and Its Role in Nitrogen Fixation. Microbes and Environments 2020, 35, ME20049 .

AMA Style

Teerana Greetatorn, Shun Hashimoto, Taro Maeda, Mitsutaka Fukudome, Pongdet Piromyou, Kamonluck Teamtisong, Panlada Tittabutr, Nantakorn Boonkerd, Masayoshi Kawaguchi, Toshiki Uchiumi, Neung Teaumroong. Mechanisms of Rice Endophytic Bradyrhizobial Cell Differentiation and Its Role in Nitrogen Fixation. Microbes and Environments. 2020; 35 (3):ME20049.

Chicago/Turabian Style

Teerana Greetatorn; Shun Hashimoto; Taro Maeda; Mitsutaka Fukudome; Pongdet Piromyou; Kamonluck Teamtisong; Panlada Tittabutr; Nantakorn Boonkerd; Masayoshi Kawaguchi; Toshiki Uchiumi; Neung Teaumroong. 2020. "Mechanisms of Rice Endophytic Bradyrhizobial Cell Differentiation and Its Role in Nitrogen Fixation." Microbes and Environments 35, no. 3: ME20049.

Journal article
Published: 04 July 2019 in Antioxidants
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Flooding limits biomass production in agriculture. Leguminous plants, important agricultural crops, use atmospheric dinitrogen gas as nitrogen nutrition by symbiotic nitrogen fixation with rhizobia, but this root-nodule symbiosis is sometimes broken down by flooding of the root system. In this study, we analyzed the effect of flooding on the symbiotic system of transgenic Lotus japonicus lines which overexpressed class 1 phytoglobin (Glb1) of L. japonicus (LjGlb1-1) or ectopically expressed that of Alnus firma (AfGlb1). In the roots of wild-type plants, flooding increased nitric oxide (NO) level and expression of senescence-related genes and decreased nitrogenase activity; in the roots of transgenic lines, these effects were absent or less pronounced. The decrease of chlorophyll content in leaves and the increase of reactive oxygen species (ROS) in roots and leaves caused by flooding were also suppressed in these lines. These results suggest that increased levels of Glb1 help maintain nodule symbiosis under flooding by scavenging NO and controlling ROS.

ACS Style

Mitsutaka Fukudome; Eri Watanabe; Ken-Ichi Osuki; Nahoko Uchi; Toshiki Uchiumi. Ectopic or Over-Expression of Class 1 Phytoglobin Genes Confers Flooding Tolerance to the Root Nodules of Lotus japonicus by Scavenging Nitric Oxide. Antioxidants 2019, 8, 206 .

AMA Style

Mitsutaka Fukudome, Eri Watanabe, Ken-Ichi Osuki, Nahoko Uchi, Toshiki Uchiumi. Ectopic or Over-Expression of Class 1 Phytoglobin Genes Confers Flooding Tolerance to the Root Nodules of Lotus japonicus by Scavenging Nitric Oxide. Antioxidants. 2019; 8 (7):206.

Chicago/Turabian Style

Mitsutaka Fukudome; Eri Watanabe; Ken-Ichi Osuki; Nahoko Uchi; Toshiki Uchiumi. 2019. "Ectopic or Over-Expression of Class 1 Phytoglobin Genes Confers Flooding Tolerance to the Root Nodules of Lotus japonicus by Scavenging Nitric Oxide." Antioxidants 8, no. 7: 206.

Journal article
Published: 01 January 2019 in Microbes and Environments
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Aphids have a mutualistic relationship with the bacterial endosymbiont Buchnera aphidicola. We previously reported seven cysteine-rich peptides in the pea aphid Acyrthosiphon pisum and named them Bacteriocyte-specific Cysteine-Rich (BCR) peptides; these peptides are exclusively expressed in bacteriocytes, special aphid cells that harbor symbionts. Similar symbiotic organ-specific cysteine-rich peptides identified in the root nodules of leguminous plants are named Nodule-specific Cysteine-Rich (NCR) peptides. NCR peptides target rhizobia in the nodules and are essential for symbiotic nitrogen fixation. A BacA (membrane protein) mutant of Sinorhizobium is sensitive to NCR peptides and is unable to establish symbiosis. Based on the structural and expressional similarities between BCR peptides and NCR peptides, we hypothesized that aphid BCR peptides exhibit antimicrobial activity, similar to some NCR peptides. We herein synthesized BCR peptides and investigated their antimicrobial activities and effects on the bacterial membrane of Escherichia coli. The peptides BCR1, BCR3, BCR5, and BCR8 exhibited antimicrobial activities with increased membrane permeability. An sbmA mutant of E. coli, a homolog of bacA of S. meliloti, was more sensitive to BCR peptides than the wild type. Our results suggest that BCR peptides have properties that may be required to control the endosymbiont, similar to NCR peptides in legumes.

ACS Style

Nahoko Uchi; Mitsutaka Fukudome; Narumi Nozaki; Miyuzu Suzuki; Ken-Ichi Osuki; Shuji Shigenobu; Toshiki Uchiumi. Antimicrobial Activities of Cysteine-rich Peptides Specific to Bacteriocytes of the Pea Aphid Acyrthosiphon pisum. Microbes and Environments 2019, 34, 155 -160.

AMA Style

Nahoko Uchi, Mitsutaka Fukudome, Narumi Nozaki, Miyuzu Suzuki, Ken-Ichi Osuki, Shuji Shigenobu, Toshiki Uchiumi. Antimicrobial Activities of Cysteine-rich Peptides Specific to Bacteriocytes of the Pea Aphid Acyrthosiphon pisum. Microbes and Environments. 2019; 34 (2):155-160.

Chicago/Turabian Style

Nahoko Uchi; Mitsutaka Fukudome; Narumi Nozaki; Miyuzu Suzuki; Ken-Ichi Osuki; Shuji Shigenobu; Toshiki Uchiumi. 2019. "Antimicrobial Activities of Cysteine-rich Peptides Specific to Bacteriocytes of the Pea Aphid Acyrthosiphon pisum." Microbes and Environments 34, no. 2: 155-160.

Journal article
Published: 28 December 2018 in Plant and Cell Physiology
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The class 1 phytoglobin, LjGlb1-1, is expressed in various tissues of the model legume Lotus japonicus, where it may play multiple functions by interacting with nitric oxide (NO). One of such functions is the onset of a proper symbiosis with Mesorhizobium loti resulting in the formation of actively N2-fixing nodules. Stable overexpression lines (Ox1 and Ox2) of LjGlb1-1 were generated and phenotyped. Both Ox lines showed reduced NO levels in roots and enhanced nitrogenase activity in mature and senescent nodules relative to the wild-type (WT). Physiological and cytological observations indicated that overexpression of LjGlb1-1 delayed nodule senescence. The application to WT nodules of the NO donor S-nitroso-N-acetyl-dl-penicillamine (SNAP) or the phytohormones abscisic acid (ABA) and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) repressed nitrogenase activity, induced the expression of three senescence-associated genes and caused cytological changes evidencing nodule senescence. These effects were almost completely reverted by the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide. Our results reveal that overexpression of LjGlb1-1 improves the activity of mature nodules and delays nodule senescence in the L.japonicus–M.loti symbiosis. These beneficial effects are probably mediated by the participation of LjGlb1-1 in controlling the concentration of NO that may be produced downstream in the phytohormone signaling pathway in nodules.

ACS Style

Mitsutaka Fukudome; Eri Watanabe; Ken-Ichi Osuki; Ryujiro Imaizumi; Toshio Aoki; Manuel Becana; Toshiki Uchiumi. Stably TransformedLotus japonicusPlants Overexpressing Phytoglobin LjGlb1-1 Show Decreased Nitric Oxide Levels in Roots and Nodules as Well as Delayed Nodule Senescence. Plant and Cell Physiology 2018, 60, 816 -825.

AMA Style

Mitsutaka Fukudome, Eri Watanabe, Ken-Ichi Osuki, Ryujiro Imaizumi, Toshio Aoki, Manuel Becana, Toshiki Uchiumi. Stably TransformedLotus japonicusPlants Overexpressing Phytoglobin LjGlb1-1 Show Decreased Nitric Oxide Levels in Roots and Nodules as Well as Delayed Nodule Senescence. Plant and Cell Physiology. 2018; 60 (4):816-825.

Chicago/Turabian Style

Mitsutaka Fukudome; Eri Watanabe; Ken-Ichi Osuki; Ryujiro Imaizumi; Toshio Aoki; Manuel Becana; Toshiki Uchiumi. 2018. "Stably TransformedLotus japonicusPlants Overexpressing Phytoglobin LjGlb1-1 Show Decreased Nitric Oxide Levels in Roots and Nodules as Well as Delayed Nodule Senescence." Plant and Cell Physiology 60, no. 4: 816-825.

Journal article
Published: 21 July 2016 in Journal of Experimental Botany
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Missense and null Lotus japonicus mutants allowed us to demonstrate that hemoglobin LjGlb1-1 is required for infection thread elongation and nodule formation, probably by regulating nitric oxide production in the roots.

ACS Style

Mitsutaka Fukudome; Laura Calvo-Begueria; Tomohiro Kado; Ken-Ichi Osuki; Maria Carmen Rubio; Ei-Ichi Murakami; Maki Nagata; Ken-Ichi Kucho; Niels Sandal; Jens Stougaard; Manuel Becana; Toshiki Uchiumi. Hemoglobin LjGlb1-1 is involved in nodulation and regulates the level of nitric oxide in theLotus japonicus–Mesorhizobium lotisymbiosis. Journal of Experimental Botany 2016, 67, 5275 -5283.

AMA Style

Mitsutaka Fukudome, Laura Calvo-Begueria, Tomohiro Kado, Ken-Ichi Osuki, Maria Carmen Rubio, Ei-Ichi Murakami, Maki Nagata, Ken-Ichi Kucho, Niels Sandal, Jens Stougaard, Manuel Becana, Toshiki Uchiumi. Hemoglobin LjGlb1-1 is involved in nodulation and regulates the level of nitric oxide in theLotus japonicus–Mesorhizobium lotisymbiosis. Journal of Experimental Botany. 2016; 67 (17):5275-5283.

Chicago/Turabian Style

Mitsutaka Fukudome; Laura Calvo-Begueria; Tomohiro Kado; Ken-Ichi Osuki; Maria Carmen Rubio; Ei-Ichi Murakami; Maki Nagata; Ken-Ichi Kucho; Niels Sandal; Jens Stougaard; Manuel Becana; Toshiki Uchiumi. 2016. "Hemoglobin LjGlb1-1 is involved in nodulation and regulates the level of nitric oxide in theLotus japonicus–Mesorhizobium lotisymbiosis." Journal of Experimental Botany 67, no. 17: 5275-5283.