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Toshiki Uchiumi
Graduate School of Science and Engineering, Kagoshima University, 1-21-35 Korimoto, Kagoshima 890-0065, Japan

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Journal article
Published: 17 February 2021 in Plants
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Under water deficit conditions, the essential macronutrient nitrogen becomes limited as a result of reduced dissolved nitrogen and root nitrogen uptake. An elevated nitrogen level might be able to mitigate these effects, integrated with the idea of using nitric oxide as abiotic stress tolerant inducers. In this study, we evaluated the potential of using elevated nitrogen priming prior to water shortage to mitigate plant stress through nitric oxide accumulation. We grew rice plants in 300 mg L−1 nitrogen for 10 weeks, then we primed plants with four different nitrogen concentrations: 100, 300 (control), 500 and 1000 mg L−1 nitrogen prior to inducing water deficit conditions. Plants primed with 500 mg L−1 nitrogen possessed a higher photosynthetic rate, relative water content, electrolyte leakage and lipid peroxidation under water deficit conditions, compared to control plants. The induction of water deficit tolerance was supported with the activation of antioxidant defense system, induced by the accumulation of nitric oxide in leaves and roots of rice plants. We originally demonstrated the accumulation of nitric oxide in leaves of rice plants. The elevated nitrogen priming can be used to enhance water deficit tolerance in irrigated paddy fields, instead of nitric oxide donors.

ACS Style

Kamolchanok Umnajkitikorn; Mitsutaka Fukudome; Toshiki Uchiumi; Neung Teaumroong. Elevated Nitrogen Priming Induced Oxinitro-Responses and Water Deficit Tolerance in Rice. Plants 2021, 10, 381 .

AMA Style

Kamolchanok Umnajkitikorn, Mitsutaka Fukudome, Toshiki Uchiumi, Neung Teaumroong. Elevated Nitrogen Priming Induced Oxinitro-Responses and Water Deficit Tolerance in Rice. Plants. 2021; 10 (2):381.

Chicago/Turabian Style

Kamolchanok Umnajkitikorn; Mitsutaka Fukudome; Toshiki Uchiumi; Neung Teaumroong. 2021. "Elevated Nitrogen Priming Induced Oxinitro-Responses and Water Deficit Tolerance in Rice." Plants 10, no. 2: 381.

Journal article
Published: 07 February 2020 in Antioxidants
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Reactive sulfur species (RSS) function as strong antioxidants and are involved in various biological responses in animals and bacteria. Few studies; however, have examined RSS in plants. In the present study, we clarified that RSS are involved in root nodule symbiosis in the model legume Lotus japonicus. Polysulfides, a type of RSS, were detected in the roots by using a sulfane sulfur-specific fluorescent probe, SSP4. Supplying the sulfane sulfur donor Na2S3 to the roots increased the amounts of both polysulfides and hydrogen sulfide (H2S) in the roots and simultaneously decreased the amounts of nitric oxide (NO) and reactive oxygen species (ROS). RSS were also detected in infection threads in the root hairs and in infected cells of nodules. Supplying the sulfane sulfur donor significantly increased the numbers of infection threads and nodules. When nodules were immersed in the sulfane sulfur donor, their nitrogenase activity was significantly reduced, without significant changes in the amounts of NO, ROS, and H2S. These results suggest that polysulfides interact with signal molecules such as NO, ROS, and H2S in root nodule symbiosis in L. japonicus. SSP4 and Na2S3 are useful tools for study of RSS in plants.

ACS Style

Mitsutaka Fukudome; Hazuki Shimada; Nahoko Uchi; Ken-Ichi Osuki; Haruka Ishizaki; Ei-Ichi Murakami; Masayoshi Kawaguchi; Toshiki Uchiumi. Reactive Sulfur Species Interact with Other Signal Molecules in Root Nodule Symbiosis in Lotus japonicus. Antioxidants 2020, 9, 145 .

AMA Style

Mitsutaka Fukudome, Hazuki Shimada, Nahoko Uchi, Ken-Ichi Osuki, Haruka Ishizaki, Ei-Ichi Murakami, Masayoshi Kawaguchi, Toshiki Uchiumi. Reactive Sulfur Species Interact with Other Signal Molecules in Root Nodule Symbiosis in Lotus japonicus. Antioxidants. 2020; 9 (2):145.

Chicago/Turabian Style

Mitsutaka Fukudome; Hazuki Shimada; Nahoko Uchi; Ken-Ichi Osuki; Haruka Ishizaki; Ei-Ichi Murakami; Masayoshi Kawaguchi; Toshiki Uchiumi. 2020. "Reactive Sulfur Species Interact with Other Signal Molecules in Root Nodule Symbiosis in Lotus japonicus." Antioxidants 9, no. 2: 145.

Journal article
Published: 04 July 2019 in Antioxidants
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Flooding limits biomass production in agriculture. Leguminous plants, important agricultural crops, use atmospheric dinitrogen gas as nitrogen nutrition by symbiotic nitrogen fixation with rhizobia, but this root-nodule symbiosis is sometimes broken down by flooding of the root system. In this study, we analyzed the effect of flooding on the symbiotic system of transgenic Lotus japonicus lines which overexpressed class 1 phytoglobin (Glb1) of L. japonicus (LjGlb1-1) or ectopically expressed that of Alnus firma (AfGlb1). In the roots of wild-type plants, flooding increased nitric oxide (NO) level and expression of senescence-related genes and decreased nitrogenase activity; in the roots of transgenic lines, these effects were absent or less pronounced. The decrease of chlorophyll content in leaves and the increase of reactive oxygen species (ROS) in roots and leaves caused by flooding were also suppressed in these lines. These results suggest that increased levels of Glb1 help maintain nodule symbiosis under flooding by scavenging NO and controlling ROS.

ACS Style

Mitsutaka Fukudome; Eri Watanabe; Ken-Ichi Osuki; Nahoko Uchi; Toshiki Uchiumi. Ectopic or Over-Expression of Class 1 Phytoglobin Genes Confers Flooding Tolerance to the Root Nodules of Lotus japonicus by Scavenging Nitric Oxide. Antioxidants 2019, 8, 206 .

AMA Style

Mitsutaka Fukudome, Eri Watanabe, Ken-Ichi Osuki, Nahoko Uchi, Toshiki Uchiumi. Ectopic or Over-Expression of Class 1 Phytoglobin Genes Confers Flooding Tolerance to the Root Nodules of Lotus japonicus by Scavenging Nitric Oxide. Antioxidants. 2019; 8 (7):206.

Chicago/Turabian Style

Mitsutaka Fukudome; Eri Watanabe; Ken-Ichi Osuki; Nahoko Uchi; Toshiki Uchiumi. 2019. "Ectopic or Over-Expression of Class 1 Phytoglobin Genes Confers Flooding Tolerance to the Root Nodules of Lotus japonicus by Scavenging Nitric Oxide." Antioxidants 8, no. 7: 206.

Journal article
Published: 07 March 2016 in Journal of Plant Research
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Phytohormone abscisic acid (ABA) inhibits root nodule formation of leguminous plants. LjGlu1, a β-1,3-glucanase gene of Lotus japonicus, has been identified as an ABA responsive gene. RNA interference of LjGlu1 increased nodule number. This suggests that LjGlu1 is involved in the regulation of nodule formation. Host legumes control nodule number by autoregulation of nodulation (AON), in which the presence of existing root nodules inhibits further nodulation. For further characterization of LjGlu1, we focused on the expression of LjGlu1 in relation to AON. In a split-root system, LjGlu1 expression peaked when AON was fully induced. Hairy roots transformed with LjCLE-RS1, a gene that induces AON, were generated. Expression of LjGlu1 was greater in the transgenic roots than in untransformed roots. LjGlu1 was not induced in a hypernodulating mutant inoculated with Mesorhizobium loti. These results suggest that the expression of LjGlu1 is involved in the system of AON. However, neither hypernodulation nor enlarged nodulation zone was observed on the transgenic hairy roots carrying LjGlu1-RNAi, suggesting that LjGlu1 is not a key player of AON. Recombinant LjGlu1 showed endo-β-1,3-glucanase activity. LjGlu1-mOrange fusion protein suggested that LjGlu1 associated with M. loti on the root hairs. Exogenous β-1,3-glucanase inhibited infection thread formation by both the wild type and the mutant, and nodule numbers were reduced. These results suggest that LjGlu1 is expressed in response to M. loti infection and functions outside root tissues, resulting in the inhibition of infection.

ACS Style

Ken-Ichi Osuki; Shun Hashimoto; Akihiro Suzuki; Masato Araragi; Akihito Takahara; Makiko Kurosawa; Ken-Ichi Kucho; Shiro Higashi; Mikiko Abe; Toshiki Uchiumi. Gene expression and localization of a β-1,3-glucanase of Lotus japonicus. Journal of Plant Research 2016, 129, 749 -758.

AMA Style

Ken-Ichi Osuki, Shun Hashimoto, Akihiro Suzuki, Masato Araragi, Akihito Takahara, Makiko Kurosawa, Ken-Ichi Kucho, Shiro Higashi, Mikiko Abe, Toshiki Uchiumi. Gene expression and localization of a β-1,3-glucanase of Lotus japonicus. Journal of Plant Research. 2016; 129 (4):749-758.

Chicago/Turabian Style

Ken-Ichi Osuki; Shun Hashimoto; Akihiro Suzuki; Masato Araragi; Akihito Takahara; Makiko Kurosawa; Ken-Ichi Kucho; Shiro Higashi; Mikiko Abe; Toshiki Uchiumi. 2016. "Gene expression and localization of a β-1,3-glucanase of Lotus japonicus." Journal of Plant Research 129, no. 4: 749-758.

Journal article
Published: 19 July 2014 in BMC Genomics
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Frankia is a genus of soil actinobacteria forming nitrogen-fixing root-nodule symbiotic relationships with non-leguminous woody plant species, collectively called actinorhizals, from eight dicotyledonous families. Frankia strains are classified into four host-specificity groups (HSGs), each of which exhibits a distinct host range. Genome sizes of representative strains of Alnus, Casuarina, and Elaeagnus HSGs are highly diverged and are positively correlated with the size of their host ranges. The content and size of 12 Frankia genomes were investigated by in silico comparative genome hybridization and pulsed-field gel electrophoresis, respectively. Data were collected from four query strains of each HSG and compared with those of reference strains possessing completely sequenced genomes. The degree of difference in genome content between query and reference strains varied depending on HSG. Elaeagnus query strains were missing the greatest number (22–32%) of genes compared with the corresponding reference genome; Casuarina query strains lacked the fewest (0–4%), with Alnus query strains intermediate (14–18%). In spite of the remarkable gene loss, genome sizes of Alnus and Elaeagnus query strains were larger than would be expected based on total length of the absent genes. In contrast, Casuarina query strains had smaller genomes than expected. The positive correlation between genome size and host range held true across all investigated strains, supporting the hypothesis that size and genome content differences are responsible for observed diversity in host plants and host plant biogeography among Frankia strains. In addition, our results suggest that different dynamics of shuffling of genome content have contributed to these symbiotic and biogeographic adaptations. Elaeagnus strains, and to a lesser extent Alnus strains, have gained and lost many genes to adapt to a wide range of environments and host plants. Conversely, rather than acquiring new genes, Casuarina strains have discarded genes to reduce genome size, suggesting an evolutionary orientation towards existence as specialist symbionts.

ACS Style

Ken-Ichi Kucho; Takashi Yamanaka; Hideo Sasakawa; Samira R Mansour; Toshiki Uchiumi. Different dynamics of genome content shuffling among host-specificity groups of the symbiotic actinobacterium Frankia. BMC Genomics 2014, 15, 1 -12.

AMA Style

Ken-Ichi Kucho, Takashi Yamanaka, Hideo Sasakawa, Samira R Mansour, Toshiki Uchiumi. Different dynamics of genome content shuffling among host-specificity groups of the symbiotic actinobacterium Frankia. BMC Genomics. 2014; 15 (1):1-12.

Chicago/Turabian Style

Ken-Ichi Kucho; Takashi Yamanaka; Hideo Sasakawa; Samira R Mansour; Toshiki Uchiumi. 2014. "Different dynamics of genome content shuffling among host-specificity groups of the symbiotic actinobacterium Frankia." BMC Genomics 15, no. 1: 1-12.

Journal article
Published: 01 July 2012 in Plant Signaling & Behavior
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Light is critical for supplying carbon for use in the energetically expensive process of nitrogen-fixing symbiosis between legumes and rhizobia. We recently showed that root nodule formation in phyB mutants [which have a constitutive shade avoidance syndrome (SAS) phenotype] was suppressed in white light, and that nodulation in wild-type is controlled by sensing the R/FR ratio through jasmonic acid (JA) signaling. We concluded that the cause of reduced root nodule formation in phyB mutants was the inhibition of JA-Ile production in root. Here we show that the shoot JA-Ile level of phyB mutants is higher than that of the wild-type strain MG20, suggesting that translocation of JA-Ile from shoot to root is impeded in the mutant. These results indicate that root nodule formation in phyB mutants is suppressed both by decreased JA-Ile production, caused by reduced JAR1 activity in root, and by reduced JA-Ile translocation from shoot to root.

ACS Style

Tamaki Shigeyama; Akiyoshi Tominaga; Susumu Arima; Tatsuya Sakai; Sayaka Inada; Yusuke Jikumaru; Yuji Kamiya; Toshiki Uchiumi; Mikiko Abe; Masatsugu Hashiguchi; Ryo Akashi; Ann M. Hirsch; Akihiro Suzuki. Additional cause for reduced JA-Ile in the root of a Lotus japonicus phyB mutant. Plant Signaling & Behavior 2012, 7, 746 -748.

AMA Style

Tamaki Shigeyama, Akiyoshi Tominaga, Susumu Arima, Tatsuya Sakai, Sayaka Inada, Yusuke Jikumaru, Yuji Kamiya, Toshiki Uchiumi, Mikiko Abe, Masatsugu Hashiguchi, Ryo Akashi, Ann M. Hirsch, Akihiro Suzuki. Additional cause for reduced JA-Ile in the root of a Lotus japonicus phyB mutant. Plant Signaling & Behavior. 2012; 7 (7):746-748.

Chicago/Turabian Style

Tamaki Shigeyama; Akiyoshi Tominaga; Susumu Arima; Tatsuya Sakai; Sayaka Inada; Yusuke Jikumaru; Yuji Kamiya; Toshiki Uchiumi; Mikiko Abe; Masatsugu Hashiguchi; Ryo Akashi; Ann M. Hirsch; Akihiro Suzuki. 2012. "Additional cause for reduced JA-Ile in the root of a Lotus japonicus phyB mutant." Plant Signaling & Behavior 7, no. 7: 746-748.