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Resveratrol is a key component of red wine and other grape products. Recent studies have characterized resveratrol as a polyphenol, and shown its beneficial effects on cancer, metabolism, and infection. This study aimed to obtain insights into the biological effects of resveratrol on myopia. To this end, we examined its anti-inflammatory influence on human retinal pigment epithelium cells and in a monocular form deprivation (MFD)-induced animal model of myopia. In MFD-induced myopia, resveratrol increased collagen I level and reduced the expression levels of matrix metalloproteinase (MMP)2, transforming growth factor (TGF)-β, and nuclear factor (NF)-κB expression levels. It also suppressed the levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β. Resveratrol exhibited no significant cytotoxicity in ARPE-19 cells. Downregulation of inflammatory cytokine production, and inhibition of AKT, c-Raf, Stat3, and NFκB phosphorylation were observed in ARPE-19 cells that were treated with resveratrol. In conclusion, the findings suggest that resveratrol inhibits inflammatory effects by blocking the relevant signaling pathways, to ameliorate myopia development. This may make it a natural candidate for drug development for myopia.
Yu-An Hsu; Chih-Sheng Chen; Yao-Chien Wang; En-Shyh Lin; Ching-Yao Chang; Jamie Chen; Ming-Yen Wu; Hui-Ju Lin; Lei Wan. Anti-Inflammatory Effects of Resveratrol on Human Retinal Pigment Cells and a Myopia Animal Model. Current Issues in Molecular Biology 2021, 43, 716 -727.
AMA StyleYu-An Hsu, Chih-Sheng Chen, Yao-Chien Wang, En-Shyh Lin, Ching-Yao Chang, Jamie Chen, Ming-Yen Wu, Hui-Ju Lin, Lei Wan. Anti-Inflammatory Effects of Resveratrol on Human Retinal Pigment Cells and a Myopia Animal Model. Current Issues in Molecular Biology. 2021; 43 (2):716-727.
Chicago/Turabian StyleYu-An Hsu; Chih-Sheng Chen; Yao-Chien Wang; En-Shyh Lin; Ching-Yao Chang; Jamie Chen; Ming-Yen Wu; Hui-Ju Lin; Lei Wan. 2021. "Anti-Inflammatory Effects of Resveratrol on Human Retinal Pigment Cells and a Myopia Animal Model." Current Issues in Molecular Biology 43, no. 2: 716-727.
The increased global incidence of myopia requires the establishment of therapeutic approaches. Previous studies have suggested that inflammation plays an important role in the development and progression of myopia. We used human retinal pigment epithelial cell to study the molecular mechanisms on how FJE and PVE lowering the inflammation of the eye. The effect of FJE and PVE in MFD induced hamster model and explore the role of inflammation cytokines in myopia. Expression levels of IL-6, IL-8, and TNF-α were upregulated in retinal pigment epithelium (RPE) cells treated with IL-6 and TNF-α. FJ extract (FJE) + PV extract (PVE) reduced IL-6, IL-8, and TNF-α expression in RPE cells. Furthermore, FJE and PVE inhibited inflammation by attenuating the phosphorylation of protein kinase B (AKT), and nuclear factor kappa-light-chain-enhancer of activated B (NF-κB) pathway. In addition, we report two resveratrol + ursolic acid compounds from FJ and PV and their inhibitory activities against IL-6, IL-8, and TNF-α expression levels in RPE cells treated with IL-6 and TNF-α. FJE, PVE, and FJE + PVE were applied to MFD hamsters and their axial length was measured after 21 days. The axial length showed statistically significant differences between phosphate-buffered saline- and FJE-, PVE-, and FJE + PVE-treated MFD eyes. FJE + PVE suppressed expressions of IL-6, IL-8, and TNF-α. They also inhibited myopia-related transforming growth factor-beta (TGF)-β1, matrix metalloproteinase (MMP)-2, and NF-κB expression while increasing type Ⅰ collagen expression. Overall, these results suggest that FJE + PVE may have a therapeutic effect on myopia and be used as a potential treatment option.
Chia-Hung Lin; Chih-Sheng Chen; Yao-Chien Wang; En-Shyh Lin; Ching-Yao Chang; Jamie Jiin-Yi Chen; Ming-Yen Wu; Hui-Ju Lin; Lei Wan. Fallopia Japonica and Prunella Vulgaris Inhibit Myopia Progression by Suppressing Akt and NFκB Mediated Inflammatory Reactions. 2021, 1 .
AMA StyleChia-Hung Lin, Chih-Sheng Chen, Yao-Chien Wang, En-Shyh Lin, Ching-Yao Chang, Jamie Jiin-Yi Chen, Ming-Yen Wu, Hui-Ju Lin, Lei Wan. Fallopia Japonica and Prunella Vulgaris Inhibit Myopia Progression by Suppressing Akt and NFκB Mediated Inflammatory Reactions. . 2021; ():1.
Chicago/Turabian StyleChia-Hung Lin; Chih-Sheng Chen; Yao-Chien Wang; En-Shyh Lin; Ching-Yao Chang; Jamie Jiin-Yi Chen; Ming-Yen Wu; Hui-Ju Lin; Lei Wan. 2021. "Fallopia Japonica and Prunella Vulgaris Inhibit Myopia Progression by Suppressing Akt and NFκB Mediated Inflammatory Reactions." , no. : 1.
Myopia is a highly prevalent refractive disorder. We investigated the effect of diacerein on monocular form deprivation (MFD) in hamsters as a possible therapeutic intervention. Diacerein is an anthraquinone derivative drug whose active metabolite is rhein. Diacerein or atropine was applied to the MFD hamsters, and their refractive error and axial length were measured after 21 days. The refractive error (control: − 0.91 ± 0.023 , atropine: − 0.3 ± 0.08 , and diacerein: − 0.27 ± 0.07 D ) and axial length (control: 0.401 ± 0.017 , atropine: 0.326 ± 0.017 , and diacerein: 0.334 ± 0.016 mm ) showed statistically significant differences between control, atropine-treated, and diacerein-treated MFD eyes. Furthermore, we determined the level of transforming growth factor-beta- (TGF-) β1, matrix metalloproteinase- (MMP-) 2, type I collagen, interleukin- (IL-) 6, IL-8, and monocyte chemoattractant protein- (MCP-) 1 in the retina. Atropine and diacerein suppressed levels of the myopia-related TGF-β1 and MMP-2 while increasing type I collagen expression. They also inhibited the interleukin IL-6, IL-8, and MCP-1 levels. Diacerein reduced the IL-6, IL-8, and MCP-1 expression in ARPE-19 cells. Furthermore, diacerein inhibited inflammation by attenuating the phosphorylation of protein kinase B (AKT) and nuclear factor kappa-light-chain-enhancer of activated B (NF-κB) pathway. This suggests that diacerein has a therapeutic effect on myopia and is a potential treatment option.
Peng-Tai Tien; Chia-Hung Lin; Chih-Sheng Chen; Ching-Yao Chang; Hsiangyu Ku; Dekang Gan; Yi-Yu Tsai; Jamie Jiin-Yi Chen; Hui-Ju Lin; Lei Wan. Diacerein Inhibits Myopia Progression through Lowering Inflammation in Retinal Pigment Epithelial Cell. Mediators of Inflammation 2021, 2021, 1 -11.
AMA StylePeng-Tai Tien, Chia-Hung Lin, Chih-Sheng Chen, Ching-Yao Chang, Hsiangyu Ku, Dekang Gan, Yi-Yu Tsai, Jamie Jiin-Yi Chen, Hui-Ju Lin, Lei Wan. Diacerein Inhibits Myopia Progression through Lowering Inflammation in Retinal Pigment Epithelial Cell. Mediators of Inflammation. 2021; 2021 ():1-11.
Chicago/Turabian StylePeng-Tai Tien; Chia-Hung Lin; Chih-Sheng Chen; Ching-Yao Chang; Hsiangyu Ku; Dekang Gan; Yi-Yu Tsai; Jamie Jiin-Yi Chen; Hui-Ju Lin; Lei Wan. 2021. "Diacerein Inhibits Myopia Progression through Lowering Inflammation in Retinal Pigment Epithelial Cell." Mediators of Inflammation 2021, no. : 1-11.
BackgroundThe increased global incidence of myopia requires the establishment of therapeutic approaches. Previous studies have suggested that inflammation plays an important role in the development and progression of myopia. This study aimed to investigate the effect of Fallopia Japonica (FJ) and Prunella Vulgaris (PV) extract on myopia caused by monocular form deprivation (MFD) and elucidate the underlying mechanisms. MethodsWe used human retinal pigment epithelial cell to study the molecular mechanisms on how FJE and PVE lowering the inflammation of the eye. The effect of FJE and PVE in MFD induced hamster model and explore the role of inflammation cytokines in myopia.ResultsMyopia progression was enhanced upon tumor necrosis factor (TNF)-α or interleukin (IL)-6 administration. Expression levels of IL-6, IL-8, and TNF-α were upregulated in retinal pigment epithelium (RPE) cells treated with IL-6 and TNF-α. FJ extract (FJE) + PV extract (PVE) reduced IL-6, IL-8, and TNF-α expression in RPE cells. Furthermore, FJE and PVE inhibited inflammation by attenuating the phosphorylation of protein kinase B (AKT), and nuclear factor kappa-light-chain-enhancer of activated B (NF-κB) pathway. In addition, we report two resveratrol + ursolic acid compounds from FJ and PV and their inhibitory activities against IL-6, IL-8, and TNF-α expression levels in RPE cells treated with IL-6 and TNF-α. FJE, PVE, and FJE + PVE were applied to MFD hamsters and their axial length was measured after 21 days. The axial length showed statistically significant differences between phosphate-buffered saline- and FJE-, PVE-, and FJE + PVE-treated MFD eyes. FJE + PVE suppressed expressions of IL-6, IL-8, and TNF-α. They also inhibited myopia-related transforming growth factor-beta (TGF)-β1, matrix metalloproteinase (MMP)-2, and NF-κB expression while increasing type Ⅰ collagen expression. ConclusionOverall, these results suggest that FJE + PVE may have a therapeutic effect on myopia and be used as a potential treatment option.
Chia-Hung Lin; Chih-Sheng Chen; Yao-Chien Wang; En-Shyh Lin; Ching-Yao Chang; Jamie Jinn-Yi Chen; Ming-Yen Wu; Hui-Ju Lin; Lei Wan. Effect of Combined Treatment With Fallopia Japonica and Prunella Vulgaris Extract on Myopia Progress. 2021, 1 .
AMA StyleChia-Hung Lin, Chih-Sheng Chen, Yao-Chien Wang, En-Shyh Lin, Ching-Yao Chang, Jamie Jinn-Yi Chen, Ming-Yen Wu, Hui-Ju Lin, Lei Wan. Effect of Combined Treatment With Fallopia Japonica and Prunella Vulgaris Extract on Myopia Progress. . 2021; ():1.
Chicago/Turabian StyleChia-Hung Lin; Chih-Sheng Chen; Yao-Chien Wang; En-Shyh Lin; Ching-Yao Chang; Jamie Jinn-Yi Chen; Ming-Yen Wu; Hui-Ju Lin; Lei Wan. 2021. "Effect of Combined Treatment With Fallopia Japonica and Prunella Vulgaris Extract on Myopia Progress." , no. : 1.
Although studies have suggested environmental factors to be triggers of headache, the contribution of long-term air pollution exposure to recurrent headaches is poorly understood. Hence, we executed this nationwide cohort study to investigate associations between levels of ambient air pollutants and risks of recurrent headaches in children in Taiwan from 2000 to 2012. We used data from the Taiwan National Health Insurance Research Database and linked them to the Taiwan Air Quality Monitoring Database. Overall, 218,008 children aged < 18 were identified from 1 January 2000, and then followed until they were diagnosed by a physician for ≥3 times with recurrent headaches or until 31 December 2012. We categorized the annual average concentration of each air pollutant (fine particulate matter, total hydrocarbon, methane, sulfur dioxide, and nitrogen dioxide) into quartiles (Q1–Q4). We measured the incidence rate, hazard ratios (HRs), and the corresponding 95% confidence intervals for recurrent headaches. stratified by the quartiles. A total of 28,037 children (12.9%) were identified with recurrent headaches. The incidence rate and adjusted HR for recurrent headaches increased with higher-level exposure of air pollutants, except sulfur dioxide. We herein demonstrate that long-term ambient air pollutant exposure might be a risk factor for childhood recurrent headaches.
Syuan-Yu Hong; Lei Wan; Hui-Ju Lin; Cheng-Li Lin; Chang-Ching Wei. Long-Term Ambient Air Pollutant Exposure and Risk of Recurrent Headache in Children: A 12-Year Cohort Study. International Journal of Environmental Research and Public Health 2020, 17, 9140 .
AMA StyleSyuan-Yu Hong, Lei Wan, Hui-Ju Lin, Cheng-Li Lin, Chang-Ching Wei. Long-Term Ambient Air Pollutant Exposure and Risk of Recurrent Headache in Children: A 12-Year Cohort Study. International Journal of Environmental Research and Public Health. 2020; 17 (23):9140.
Chicago/Turabian StyleSyuan-Yu Hong; Lei Wan; Hui-Ju Lin; Cheng-Li Lin; Chang-Ching Wei. 2020. "Long-Term Ambient Air Pollutant Exposure and Risk of Recurrent Headache in Children: A 12-Year Cohort Study." International Journal of Environmental Research and Public Health 17, no. 23: 9140.
Allergic inflammatory diseases are a global public health concern affecting millions of people. Although there are several potential hypotheses, details regarding their molecular mechanisms are still ambiguous. Recently, a group of β-galactoside-binding proteins, galectins, have been revealed as important factors in altering allergic chronic inflammatory diseases. In this review, we describe the molecular and cellular basis of how galectins modulate inflammatory reactions. We also provide an overview of clinical features related to galectins. Finally, we discuss the potential issues that might lead to misrepresentation of the exact biological functions of galectins.
Lei Wan; Yu-An Hsu; Chang-Ching Wei; Fu-Tong Liu. Galectins in allergic inflammatory diseases. Molecular Aspects of Medicine 2020, 79, 100925 .
AMA StyleLei Wan, Yu-An Hsu, Chang-Ching Wei, Fu-Tong Liu. Galectins in allergic inflammatory diseases. Molecular Aspects of Medicine. 2020; 79 ():100925.
Chicago/Turabian StyleLei Wan; Yu-An Hsu; Chang-Ching Wei; Fu-Tong Liu. 2020. "Galectins in allergic inflammatory diseases." Molecular Aspects of Medicine 79, no. : 100925.
To investigate the particle size distribution of particulate matter and the concentration of specific perfluorinated compounds in indoor dust samples from several locations. Then, we used cell-based assays to investigate the effect of perfluorinated compounds on human corneal epithelial (HCEpiC), endothelial cells (HCEC) and retinal pigment epithelial cells (RPE). Indoor dust samples were collected at five different locations and PM50–10, PM10–2.5, and PM2.5–1 were fractionized. The presence and levels of 8:2 fluorotelomer alcohol, 10:2 fluorotelomer alcohol, and perfluorooctanoic acid were detected by gas chromatography–mass spectrometry. The effect of perfluorooctanoic acid on the activation of reactive oxygen species, transepithelial resistance as well as the expression of interleukin (IL)-6 and IL-8 were determined. The basolateral media of human corneal epithelial or human corneal endothelial cells were used to treat human corneal endothelial or retinal pigment epithelial cells, respectively to indicate the potential of ocular surface inflammation may result in retinal inflammation. Among perfluorinated compounds, only perfluorooctanoic acid was detected in all indoor dust samples. Perfluorooctanoic acid had the highest concentration among all perfluorinated compounds in the samples. Exposure to perfluorooctanoic acid impaired tight junction sealing and increased the levels of reactive oxygen species in human corneal epithelial cells. In human corneal epithelial cells, secretion of IL-6 and IL-8 in both apical and basolateral media was promoted significantly by perfluorooctanoic acid treatment. Stimulation with the basolateral media from perfluorooctanoic acid-treated human corneal epithelial cells induced inflammation in human corneal endothelial cells. The treatment of retinal pigment epithelial cells with the basolateral media from stimulated human corneal endothelial cells also elicited the secretion of proinflammatory cytokines. The results indicate that perfluorooctanoic acid exposure impaired the tight junction of corneal cells and caused inflammatory reactions in the retina. Exposure of the cornea to perfluorooctanoic acid contained in particulate matter might induce oxidative stress and inflammation in the retina and represent a risk factor for age-related macular degeneration.
Peng-Tai Tien; Hui-Ju Lin; Yi-Yu Tsai; Yun-Ping Lim; Chih Sheng Chen; Ching-Yao Chang; Chao-Jen Lin; Jamie Jiin-Yi Chen; Shan-Mei Wu; Yuh-Jeen Huang; Lei Wan. Perfluorooctanoic acid in indoor particulate matter triggers oxidative stress and inflammation in corneal and retinal cells. Scientific Reports 2020, 10, 1 -12.
AMA StylePeng-Tai Tien, Hui-Ju Lin, Yi-Yu Tsai, Yun-Ping Lim, Chih Sheng Chen, Ching-Yao Chang, Chao-Jen Lin, Jamie Jiin-Yi Chen, Shan-Mei Wu, Yuh-Jeen Huang, Lei Wan. Perfluorooctanoic acid in indoor particulate matter triggers oxidative stress and inflammation in corneal and retinal cells. Scientific Reports. 2020; 10 (1):1-12.
Chicago/Turabian StylePeng-Tai Tien; Hui-Ju Lin; Yi-Yu Tsai; Yun-Ping Lim; Chih Sheng Chen; Ching-Yao Chang; Chao-Jen Lin; Jamie Jiin-Yi Chen; Shan-Mei Wu; Yuh-Jeen Huang; Lei Wan. 2020. "Perfluorooctanoic acid in indoor particulate matter triggers oxidative stress and inflammation in corneal and retinal cells." Scientific Reports 10, no. 1: 1-12.
The formation of foam cells, which are macrophages that have engulfed oxidized low-density lipoprotein (OxLDL), constitutes the first stage in the development of atherosclerosis. Previously, we found that knocking down galectin-12, a negative regulator of lipolysis, leads to reduced secretion of monocyte chemoattractant protein-1 (MCP-1), a chemokine that plays an important role in atherosclerosis. This prompted us to study the role of galectin-12 in atherosclerosis. With that aim, we examined foam cell formation in Gal12‒/‒ murine macrophages exposed to OxLDL and acetylated LDL (AcLDL). Then, we generated an LDL receptor and galectin-12 double knockout (DKO) mice and studied the effect of galectin-12 on macrophage function and atherosclerosis. Lastly, we evaluated the role of galectin-12 in human THP-1 macrophages using a doxycycline-inducible conditional knockdown system. Galectin-12 knockout significantly inhibited foam cell formation in murine macrophages through the downregulation of cluster of differentiation 36 (CD36), and the upregulation of ATP Binding Cassette Subfamily A Member 1 (ABCA1), ATP Binding Cassette Subfamily G Member 1 (ABCG1), and scavenger receptor class B type 1 (SRB1). Consistent with this, galectin-12 knockdown inhibited foam cell formation in human macrophages. In addition, the ablation of galectin-12 promoted M2 macrophage polarization in human and murine macrophages as evidenced by the upregulation of the M2 marker genes, CD206 and CD163, and downregulation of the M1 cytokines, tumor necrosis factor α (TNF- α), interleukin-6 (IL-6), and MCP-1. Moreover, the ablation of galectin-12 decreased atherosclerosis formation in DKO mice. Based on these results, we propose galectin-12 as a potential therapeutic target for atherosclerosis.
En-Shyh Lin; Yu-An Hsu; Ching-Yao Chang; Hui-Ju Lin; Chih Sheng Chen; Lei Wan. Ablation of Galectin-12 Inhibits Atherosclerosis through Enhancement of M2 Macrophage Polarization. International Journal of Molecular Sciences 2020, 21, 5511 .
AMA StyleEn-Shyh Lin, Yu-An Hsu, Ching-Yao Chang, Hui-Ju Lin, Chih Sheng Chen, Lei Wan. Ablation of Galectin-12 Inhibits Atherosclerosis through Enhancement of M2 Macrophage Polarization. International Journal of Molecular Sciences. 2020; 21 (15):5511.
Chicago/Turabian StyleEn-Shyh Lin; Yu-An Hsu; Ching-Yao Chang; Hui-Ju Lin; Chih Sheng Chen; Lei Wan. 2020. "Ablation of Galectin-12 Inhibits Atherosclerosis through Enhancement of M2 Macrophage Polarization." International Journal of Molecular Sciences 21, no. 15: 5511.
To evaluate the incidence of endometriosis among endometritis patients and its association with confounding comorbidities. A population-based, retrospective cohort study of women aged between 20 to 55 years, who were newly diagnosed with endometritis between 2000 to 2013. A total of 16,830 endometritis patients and 67,230 non-endometritis individuals were enrolled by accessing data from the National Health Insurance Research Database of Taiwan. The comorbidities accessed were uterine leiomyoma, rheumatoid arthritis, ovarian cancer, infertility and allergic diseases. The mean follow-up period was 9.15 years for the non-endometritis cohort and 9.13 years for the endometritis cohort. There were significantly higher percentages of uterine leiomyoma, rheumatoid arthritis, infertility, ovarian cancer and allergic diseases in the endometritis cohort than in the non-endometritis cohort. Patients with endometritis had a 1.5-fold increased risk of their condition advancing to endometriosis (HR 1.58, 95% CI 1.48-1.68). Our results suggest that patients with endometritis exhibited a positive correlation in developing endometriosis.
Kent Yu-Hsien Lin; Cherry Yin-Yi Chang; Wu-Chou Lin; Lei Wan. Increased risk of endometriosis in patients with endometritis - a nationwide cohort study involving 84,150 individuals. Ginekologia Polska 2020, 91, 193 -200.
AMA StyleKent Yu-Hsien Lin, Cherry Yin-Yi Chang, Wu-Chou Lin, Lei Wan. Increased risk of endometriosis in patients with endometritis - a nationwide cohort study involving 84,150 individuals. Ginekologia Polska. 2020; 91 (4):193-200.
Chicago/Turabian StyleKent Yu-Hsien Lin; Cherry Yin-Yi Chang; Wu-Chou Lin; Lei Wan. 2020. "Increased risk of endometriosis in patients with endometritis - a nationwide cohort study involving 84,150 individuals." Ginekologia Polska 91, no. 4: 193-200.
Purpose. Patients with central serous chorioretinopathy (CSC) typically present with acute visual impairment and metamorphopsia. The disease previously has been associated with psychological stress. Population-based cohort studies on the risk of CSC among patients with nonorganic sleep disturbance (NOSD) are limited. An early sign of psychiatric disorder was probably sleep disturbance. Furthermore, psychological stress may be caused by sleep disturbance. We investigated the relationship between NOSD and the incidence of CSC. Design. Longitudinal cohort study. Participants. We used the Longitudinal Health Insurance Database and collected the data of 53,743 NOSD patients without CSC between 2000 and 2005 as the study group. Four-fold controls were selected randomly from those without neither sleep disturbance nor a CSC history with frequency matching of age, sex, and index-year. Methods. The difference in sex, age group, comorbidities, and steroid use between the two groups was analyzed by the χ 2 test. Cox-proportional hazard regression was utilized to estimate the hazard ratio (HR) and 95% confidence intervals (95% CI) for comparison of the two groups. Kaplan–Meier analysis was applied to measure the cumulative incidence of CSC. Furthermore, the log-rank test was used to test the incidence difference between the two groups. Main Outcome Measures. The incidence rate of CSC in the following years until 2011 was detected. Results. During a mean follow-up of 7.36 ± 2.88 years, NOSD patients had a higher incidence of CSC than the controls (3.10 vs. 1.86 per 10,000 person-years; adjusted HR, 1.65; 95% CI, 1.34–2.02). Men had a higher risk of CSC than women. Sensitivity analyses stratified by sex, age group, or comorbidity condition showed consistently that NOSD patients had a higher risk of CSC than their controls. Dose-response showed that higher NOSD severity had even higher CSC risk. Conclusions. NOSD is an independent indicator for the increased risk of subsequent CSC development.
Peng-Tai Tien; Chien-Yu Lai; Chun-Ju Lin; Wen-Lu Chen; Po-Kang Lin; Chih-Hsin Muo; Yi-Yu Tsai; Lei Wan; Wen-Chao Ho; Hui-Ju Lin. Increased Risk of Central Serous Chorioretinopathy among Patients with Nonorganic Sleep Disturbance. Journal of Ophthalmology 2020, 2020, 1 -7.
AMA StylePeng-Tai Tien, Chien-Yu Lai, Chun-Ju Lin, Wen-Lu Chen, Po-Kang Lin, Chih-Hsin Muo, Yi-Yu Tsai, Lei Wan, Wen-Chao Ho, Hui-Ju Lin. Increased Risk of Central Serous Chorioretinopathy among Patients with Nonorganic Sleep Disturbance. Journal of Ophthalmology. 2020; 2020 ():1-7.
Chicago/Turabian StylePeng-Tai Tien; Chien-Yu Lai; Chun-Ju Lin; Wen-Lu Chen; Po-Kang Lin; Chih-Hsin Muo; Yi-Yu Tsai; Lei Wan; Wen-Chao Ho; Hui-Ju Lin. 2020. "Increased Risk of Central Serous Chorioretinopathy among Patients with Nonorganic Sleep Disturbance." Journal of Ophthalmology 2020, no. : 1-7.
Background Galectin-9 is a β-galactoside-binding protein with two carbohydrate recognition domains. Recent studies have revealed that galectin-9 regulates cellular biological reactions and plays a pivotal role in fibrosis. The aim of this study was to determine the role of galectin-9 in the pathogenesis of bleomycin-induced systemic sclerosis (SSc). Methods Human galectin-9 levels in the serum of patients with SSc and mouse sera galectin-9 levels were measured by a Bio-Plex immunoassay and enzyme-linked immunosorbent assay. Lung fibrosis was induced using bleomycin in galectin-9 wild-type and knockout mice. The effects of galectin-9 on the fibrosis markers and signaling molecules in the mouse lung tissues and primary lung fibroblast cells were assessed with western blotting and quantitative polymerase chain reaction. Results Galectin-9 levels in the serum were significantly higher (9-fold) in patients compared to those of healthy individuals. Galectin-9 deficiency in mice prominently ameliorated epithelial proliferation, collagen I accumulation, and α-smooth muscle actin expression. In addition, the galectin-9 knockout mice showed reduced protein expression levels of fibrosis markers such as Smad2/3, connective tissue growth factor, and endothelin-1. Differences between the wild-type and knockout groups were also observed in the AKT, mitogen-activated protein kinase, and c-Jun N-terminal kinase signaling pathways. Galectin-9 deficiency decreased the signal activation induced by transforming growth factor-beta in mouse primary fibroblasts, which plays a critical role in fibroblast activation and aberrant catabolism of the extracellular matrix. Conclusions Our findings suggest that lack of galectin-9 protects against bleomycin-induced SSc. Moreover, galectin-9 might be involved in regulating the progression of fibrosis in multiple pathways.
Yu-An Hsu; Ching-Yao Chang; Joung-Liang Lan; Ju-Pi Li; Hui-Ju Lin; Chih-Sheng Chen; Lei Wan; Fu-Tong Liu. Amelioration of bleomycin-induced pulmonary fibrosis via TGF-β-induced Smad and non-Smad signaling pathways in galectin-9-deficient mice and fibroblast cells. Journal of Biomedical Science 2020, 27, 1 -9.
AMA StyleYu-An Hsu, Ching-Yao Chang, Joung-Liang Lan, Ju-Pi Li, Hui-Ju Lin, Chih-Sheng Chen, Lei Wan, Fu-Tong Liu. Amelioration of bleomycin-induced pulmonary fibrosis via TGF-β-induced Smad and non-Smad signaling pathways in galectin-9-deficient mice and fibroblast cells. Journal of Biomedical Science. 2020; 27 (1):1-9.
Chicago/Turabian StyleYu-An Hsu; Ching-Yao Chang; Joung-Liang Lan; Ju-Pi Li; Hui-Ju Lin; Chih-Sheng Chen; Lei Wan; Fu-Tong Liu. 2020. "Amelioration of bleomycin-induced pulmonary fibrosis via TGF-β-induced Smad and non-Smad signaling pathways in galectin-9-deficient mice and fibroblast cells." Journal of Biomedical Science 27, no. 1: 1-9.
Myopia is caused by complex genetic and environmental factors. However, information regarding the effect of long-term exposure to air pollutants on the risk of development of myopia is lacking. We collected data from two linked databases: the Taiwan National Health Insurance Research Database (NHIRD) and the Taiwan Air Quality-Monitoring Database (TAQMD). A total of 15,822 children (16.3%) were diagnosed with myopia within the cohort. The incidence rate of myopia increased with exposure to increasing concentrations of particulate matter (PM2.5) and nitrogen oxides (NOx), increasing from 15.8 to 24.5 and from 13.7 to 34.4, per 1000 person-years, respectively. The adjusted hazard ratio for myopia increased with elevated PM2.5 and NOx exposure concentrations in Q4 to 1.57 and 2.60, respectively, compared to those exposed to the corresponding concentrations in Q1. In the animal experiments, PM2.5 induced myopia in hamsters by enhancing inflammation and was inhibited by resveratrol treatment compared to the control group. The change in axial length in the PM2.5 group was 0.386 ± 0.069 mm versus 0.287 ± 0.086 mm in the control group and 0.257 ± 0.059 mm in the PM2.5 + resveratrol group. We provide both clinical and experimental correlations that exposure to ambient air pollutants is associated with the pathogenesis of myopia.
Chang-Ching Wei; Hui-Ju Lin; Yun-Ping Lim; Chih-Sheng Chen; Ching-Yao Chang; Chao-Jen Lin; Jamie Jinn-Yi Chen; Peng-Tai Tien; Cheng-Li Lin; Lei Wan. PM2.5 and NOx exposure promote myopia: clinical evidence and experimental proof. Environmental Pollution 2019, 254, 113031 .
AMA StyleChang-Ching Wei, Hui-Ju Lin, Yun-Ping Lim, Chih-Sheng Chen, Ching-Yao Chang, Chao-Jen Lin, Jamie Jinn-Yi Chen, Peng-Tai Tien, Cheng-Li Lin, Lei Wan. PM2.5 and NOx exposure promote myopia: clinical evidence and experimental proof. Environmental Pollution. 2019; 254 ():113031.
Chicago/Turabian StyleChang-Ching Wei; Hui-Ju Lin; Yun-Ping Lim; Chih-Sheng Chen; Ching-Yao Chang; Chao-Jen Lin; Jamie Jinn-Yi Chen; Peng-Tai Tien; Cheng-Li Lin; Lei Wan. 2019. "PM2.5 and NOx exposure promote myopia: clinical evidence and experimental proof." Environmental Pollution 254, no. : 113031.
Objective: To evaluate the suicide rate among patients oophorectomized for benign conditions and its association with confounding comorbidities. Method: We conducted a population-based, retrospective cohort study of women aged ≥20 years that underwent oophorectomy including unilateral or bilateral in laparotomy or laparoscopy for benign conditions during 2000–2013. A total of 145,588 oophorectomized and 582,352 non-oophorectomized women were included with an average follow-up time of 7 years. The comorbidities assessed were hypertension (HTN), diabetes mellitus, hyperlipidemia, stroke, chronic obstructive pulmonary disease (COPD), chronic liver disease and cirrhosis, chronic kidney disease and anxiety disorder. Result: The overall suicide rate was significantly higher in the oophorectomized group. The rate among oophorectomized patients of 20–49 years was significantly greater than in non-oophorectomized patients of the same age group. Hypertension, COPD, anxiety disorder and chronic liver disease and cirrhosis were associated with a significantly higher suicide rate in oophorectomized women. A significant increase in suicide incidence was observed in patients with <6 years’ follow-up. Conclusion: A significant increase in suicide rate among oophorectomized women aged 20–49 years was found. The decision to perform oophorectomy should be made cautiously, especially in patients with hypertension, COPD, chronic liver disease and cirrhosis or anxiety disorder. Patients should be followed for at least 6 years postoperatively since the suicide rate is significantly higher in this period.
Li-Wei Chiu; Kent Yu-Hsien Lin; Cherry Yin-Yi Chang; Wu-Chou Lin; Lei Wan. Increased suicide risk among patients oophorectomized following benign conditions and its association with comorbidities. Journal of Psychosomatic Obstetrics & Gynecology 2019, 41, 137 -143.
AMA StyleLi-Wei Chiu, Kent Yu-Hsien Lin, Cherry Yin-Yi Chang, Wu-Chou Lin, Lei Wan. Increased suicide risk among patients oophorectomized following benign conditions and its association with comorbidities. Journal of Psychosomatic Obstetrics & Gynecology. 2019; 41 (2):137-143.
Chicago/Turabian StyleLi-Wei Chiu; Kent Yu-Hsien Lin; Cherry Yin-Yi Chang; Wu-Chou Lin; Lei Wan. 2019. "Increased suicide risk among patients oophorectomized following benign conditions and its association with comorbidities." Journal of Psychosomatic Obstetrics & Gynecology 41, no. 2: 137-143.
Chang-Ching Wei; Yung-Jen Kung; Chih Sheng Chen; Ching-Yao Chang; Chao-Jen Lin; Peng-Tai Tien; Hsing-Yi Chang; Hsuan-Ju Chen; Yong-San Huang; Hui-Ju Lin; Lei Wan. Corrigendum to “Allergic Conjunctivitis-induced Retinal Inflammation Promotes Myopia Progression” [EBioMedicine 28 (2018) 274–286]. EBioMedicine 2019, 41, 717 -718.
AMA StyleChang-Ching Wei, Yung-Jen Kung, Chih Sheng Chen, Ching-Yao Chang, Chao-Jen Lin, Peng-Tai Tien, Hsing-Yi Chang, Hsuan-Ju Chen, Yong-San Huang, Hui-Ju Lin, Lei Wan. Corrigendum to “Allergic Conjunctivitis-induced Retinal Inflammation Promotes Myopia Progression” [EBioMedicine 28 (2018) 274–286]. EBioMedicine. 2019; 41 ():717-718.
Chicago/Turabian StyleChang-Ching Wei; Yung-Jen Kung; Chih Sheng Chen; Ching-Yao Chang; Chao-Jen Lin; Peng-Tai Tien; Hsing-Yi Chang; Hsuan-Ju Chen; Yong-San Huang; Hui-Ju Lin; Lei Wan. 2019. "Corrigendum to “Allergic Conjunctivitis-induced Retinal Inflammation Promotes Myopia Progression” [EBioMedicine 28 (2018) 274–286]." EBioMedicine 41, no. : 717-718.
Nonalcoholic fatty liver disease (NAFLD) is a very common liver disease, and its incidence has significantly increased worldwide. The liver X receptor α (LXRα) is a multifunctional nuclear receptor that controls lipid homeostasis. Inhibition of LXRα transactivation may be beneficial for NAFLD and hyperlipidemia treatment. Ursolic acid (UA) is a plant triterpenoid with many beneficial effects; however, the mechanism of its action on LXRα remains elusive. We evaluated the effects of UA on T0901317 (T090)-induced LXRα activation and steatosis. UA significantly decreased the LXR response element and sterol regulatory element-binding protein-1c (SREBP-1c) gene promoter activities, mRNA, protein expression of LXRα target genes, and hepatic cellular lipid content in a T090-induced mouse model. A molecular docking study indicated that UA bound competitively with T090 at the LXRα ligand binding domain. UA stimulated AMP-activated protein kinase (AMPK) phosphorylation in hepatic cells and increased corepressor, small heterodimer partner-interacting leucine zipper protein (SMILE) but decreased coactivator, steroid receptor coactivator-1 (SRC-1) recruitment to the SREBP-1c promoter region. In contrast, UA induced SRC-1 binding but decreased SMILE binding to reverse cholesterol transport-related gene promoters in intestinal cells, increasing lipid excretion from intestinal cells. Additionally, UA reduced valproate-induced LXRα mediated and rifampin-induced pregnane X receptor mediated lipogenesis, offering potential treatments for drug-induced hepatic steatosis. Thus, UA displays liver specificity and can be selectively repressed while RCT stimulation by LXRα is preserved and enhanced. This is a novel therapeutic option to treat NAFLD and may be helpful in developing LXR agonists to prevent atherosclerosis.
Yen-Ning Lin; Charles C. N. Wang; Hsiao-Yun Chang; Fang-Yi Chu; Yu-An Hsu; Wai-Kok Cheng; Wei-Chih Ma; Chao-Jung Chen; Lei Wan; Yun-Ping Lim. Ursolic Acid, a Novel Liver X Receptor α (LXRα) Antagonist Inhibiting Ligand-Induced Nonalcoholic Fatty Liver and Drug-Induced Lipogenesis. Journal of Agricultural and Food Chemistry 2018, 66, 11647 -11662.
AMA StyleYen-Ning Lin, Charles C. N. Wang, Hsiao-Yun Chang, Fang-Yi Chu, Yu-An Hsu, Wai-Kok Cheng, Wei-Chih Ma, Chao-Jung Chen, Lei Wan, Yun-Ping Lim. Ursolic Acid, a Novel Liver X Receptor α (LXRα) Antagonist Inhibiting Ligand-Induced Nonalcoholic Fatty Liver and Drug-Induced Lipogenesis. Journal of Agricultural and Food Chemistry. 2018; 66 (44):11647-11662.
Chicago/Turabian StyleYen-Ning Lin; Charles C. N. Wang; Hsiao-Yun Chang; Fang-Yi Chu; Yu-An Hsu; Wai-Kok Cheng; Wei-Chih Ma; Chao-Jung Chen; Lei Wan; Yun-Ping Lim. 2018. "Ursolic Acid, a Novel Liver X Receptor α (LXRα) Antagonist Inhibiting Ligand-Induced Nonalcoholic Fatty Liver and Drug-Induced Lipogenesis." Journal of Agricultural and Food Chemistry 66, no. 44: 11647-11662.
Endometriosis occurs when endometrial tissue exists outside the uterine cavity. The presence of ectopic endometrial tissue and resultant inflammation cause serious symptoms, including chronic pelvic pain, dysmenorrhea, dyspareunia, and infertility. Pelvic inflammatory disease is caused by the ascension of pathogenic bacteria from the vagina to the uterus, Fallopian tubes, and ovaries. The factors involved in the pathogenesis of the two conditions are not clearly understood, but recent studies have suggested that disturbances of the female reproductive tract microbiota and inflammatory processes influence the development of both diseases. Using data from the National Health Insurance Research Database (NHIRD), we conducted a study to assess the association of pelvic inflammatory disease (PID) with endometriosis. An age-matched control group including patients without PID was selected. Patients with a pre-existing diagnosis of endometriosis were excluded. This nationwide retrospective cohort study, involving a total of 141,460 patients, demonstrated that patients with PID had a three-fold increase in the risk of developing endometriosis (HR = 3.02, 95% CI = 2.85–3.2).
Fei-Wu Tai; Cherry Yin-Yi Chang; Jen-Huai Chiang; Wu-Chou Lin; Lei Wan. Association of Pelvic Inflammatory Disease with Risk of Endometriosis: A Nationwide Cohort Study Involving 141,460 Individuals. Journal of Clinical Medicine 2018, 7, 379 .
AMA StyleFei-Wu Tai, Cherry Yin-Yi Chang, Jen-Huai Chiang, Wu-Chou Lin, Lei Wan. Association of Pelvic Inflammatory Disease with Risk of Endometriosis: A Nationwide Cohort Study Involving 141,460 Individuals. Journal of Clinical Medicine. 2018; 7 (11):379.
Chicago/Turabian StyleFei-Wu Tai; Cherry Yin-Yi Chang; Jen-Huai Chiang; Wu-Chou Lin; Lei Wan. 2018. "Association of Pelvic Inflammatory Disease with Risk of Endometriosis: A Nationwide Cohort Study Involving 141,460 Individuals." Journal of Clinical Medicine 7, no. 11: 379.
Atropine and orthokeratology (OK) are both effective in slowing the progression of myopia. In the current study, we studied the combined effects of atropine and OK lenses on slowing the progression of myopia. This retrospective study included 84 patients who wore OK lenses and received atropine treatment (OA) and 95 patients who wore OK lenses alone (OK) for 2 years. We stratified patients into low (<6 D, LM) and high (≥6 D, HM) myopia groups, as well as two different atropine concentrations (0.125% and 0.025%). Significantly better LM control was observed in OA1 patients, compared with OK1 patients. Axial length was significantly shorter in the OA1 group (24.67 ± 1.53 mm) than in the OK1 group (24.9 ± 1.98 mm) (p = 0.042); similarly, it was shorter in the OA2 group (24.73 ± 1.53 mm) than in the OK2 group (25.01 ± 1.26 mm) (p = 0.031). For the HM patients, OA3 patients compared with OK3 patients, axial length was significantly shorter in the OA3 group (25.78 ± 1.46 mm) than in the OK3 group (25.93 ± 1.94 mm) (p = 0.021); similarly, it was shorter in the OA4 patients (25.86 ± 1.21 mm) than in the OK4 patients (26.05 ± 1.57 mm) (p = 0.011). Combined treatment with atropine and OK lenses would be a choice of treatment to control the development of myopia.
Lei Wan; Chang-Ching Wei; Chih Sheng Chen; Ching-Yao Chang; Chao-Jen Lin; Jamie Jiin-Yi Chen; Peng-Tai Tien; Hui-Ju Lin. The Synergistic Effects of Orthokeratology and Atropine in Slowing the Progression of Myopia. Journal of Clinical Medicine 2018, 7, 259 .
AMA StyleLei Wan, Chang-Ching Wei, Chih Sheng Chen, Ching-Yao Chang, Chao-Jen Lin, Jamie Jiin-Yi Chen, Peng-Tai Tien, Hui-Ju Lin. The Synergistic Effects of Orthokeratology and Atropine in Slowing the Progression of Myopia. Journal of Clinical Medicine. 2018; 7 (9):259.
Chicago/Turabian StyleLei Wan; Chang-Ching Wei; Chih Sheng Chen; Ching-Yao Chang; Chao-Jen Lin; Jamie Jiin-Yi Chen; Peng-Tai Tien; Hui-Ju Lin. 2018. "The Synergistic Effects of Orthokeratology and Atropine in Slowing the Progression of Myopia." Journal of Clinical Medicine 7, no. 9: 259.
Japanese encephalitis virus (JEV) non-structural protein 5 (NS5) exhibits type I interferon (IFN) antagonists, contributing to immune escape, and even inducing viral anti-apoptosis. This study investigated the anti-apoptotic mechanism of JEV NS5 protein on type I IFN-induced apoptosis of human medulloblastoma cells. Vector control and NS5-expressing cells were treated with IFN-β, and then harvested for analyzing apoptotic pathways with flow cytometry, Western blotting, subcellular localization, etc. RESULTS: Annexin V-FITC/PI staining indicated that IFN-β triggered apoptosis of human medulloblastoma cells, but JEV NS5 protein significantly inhibited IFN-β-induced apoptosis. Phage display technology and co-immunoprecipitation assay identified the anti-apoptotic protein Hsp70 as a NS5-interacting protein. In addition, Western blotting demonstrated that NS5 protein up-regulated the Hsp70 expression, and reduced IFN-β-induced phosphorylation of ERK2, p38 MAPK and STAT1. Hsp70 down-regulation by quercetin significantly recovered IFN-β-induced apoptosis of NS5-expressing cells, correlating with the increase in the phosphorylation of ERK2, p38 MAPK, and STAT1. Inhibiting the ATPase activity of Hsp70 by VER-155008 resulted in the elevated IFN-β-induced apoptosis in vector control and NS5-expressing cells. The results indicated Hsp70 up-regulation by JEV NS5 not only involved in type I IFN antagonism, but also responded to the anti-apoptotic action of JEV NS5 protein through the blocking IFN-β-induced p38 MAPK/STAT1-mediated apoptosis.
Jing-Ru Weng; Chun-Hung Hua; Chao-Hsien Chen; Su-Hua Huang; Ching-Ying Wang; Ying-Ju Lin; Lei Wan; Cheng-Wen Lin. Anti-apoptotic activity of Japanese encephalitis virus NS5 protein in human medulloblastoma cells treated with interferon-β. Journal of Microbiology, Immunology and Infection 2018, 51, 456 -464.
AMA StyleJing-Ru Weng, Chun-Hung Hua, Chao-Hsien Chen, Su-Hua Huang, Ching-Ying Wang, Ying-Ju Lin, Lei Wan, Cheng-Wen Lin. Anti-apoptotic activity of Japanese encephalitis virus NS5 protein in human medulloblastoma cells treated with interferon-β. Journal of Microbiology, Immunology and Infection. 2018; 51 (4):456-464.
Chicago/Turabian StyleJing-Ru Weng; Chun-Hung Hua; Chao-Hsien Chen; Su-Hua Huang; Ching-Ying Wang; Ying-Ju Lin; Lei Wan; Cheng-Wen Lin. 2018. "Anti-apoptotic activity of Japanese encephalitis virus NS5 protein in human medulloblastoma cells treated with interferon-β." Journal of Microbiology, Immunology and Infection 51, no. 4: 456-464.
Chia-Wen Lo; Chih-Sheng Chen; Ying-Chi Chen; Yu-An Hsu; Chi-Chun Huang; Ching-Yao Chang; Chao-Jen Lin; Cheng-Wen Lin; Hui-Ju Lin; Fu-Tong Liu; Lei Wan. Back cover: Allyl Isothiocyanate Ameliorates Obesity by Inhibiting Galectin-12. Molecular Nutrition & Food Research 2018, 62, 1 .
AMA StyleChia-Wen Lo, Chih-Sheng Chen, Ying-Chi Chen, Yu-An Hsu, Chi-Chun Huang, Ching-Yao Chang, Chao-Jen Lin, Cheng-Wen Lin, Hui-Ju Lin, Fu-Tong Liu, Lei Wan. Back cover: Allyl Isothiocyanate Ameliorates Obesity by Inhibiting Galectin-12. Molecular Nutrition & Food Research. 2018; 62 (6):1.
Chicago/Turabian StyleChia-Wen Lo; Chih-Sheng Chen; Ying-Chi Chen; Yu-An Hsu; Chi-Chun Huang; Ching-Yao Chang; Chao-Jen Lin; Cheng-Wen Lin; Hui-Ju Lin; Fu-Tong Liu; Lei Wan. 2018. "Back cover: Allyl Isothiocyanate Ameliorates Obesity by Inhibiting Galectin-12." Molecular Nutrition & Food Research 62, no. 6: 1.
: The aim of this study was to investigate the signaling pathways by which allyl isothiocyanate (AITC) reduces adipocyte differentiation and the efficacy of AITC in suppressing galectin-12 levels as a therapeutic for high fat diet (HFD)-induced obesity. : AITC presents anti-adipogenic effects on 3T3-L1 cells by decreasing lipid droplet accumulation in a dose-dependent manner. AITC suppresses 3T3-L1 differentiation into adipocytes by decreasing galectin-12 expression and by down-regulating key adipogenic transcription factors. AITC influences the expression of 3T3-L1 pre-adipocytes by modulating adipokine expression (leptin and resistin) and by regulating the protein kinase B (PKB/Akt)/cAMP response element-binding protein (CREB) pathway. In HFD-fed mice, oral administration of AITC reduced the body weight, accumulation of lipid droplets in the liver, and white adipocyte size. : In summary, our results indicate that AITC inhibits adipocyte differentiation by suppressing galectin-12 levels in 3T3L1 cells and has anti-obesity effects in HFD-fed mice. This article is protected by copyright. All rights reserved
Chia-Wen Lo; Chih-Sheng Chen; Ying-Chi Chen; Yu-An Hsu; Chi-Chun Huang; Ching-Yao Chang; Chao-Jen Lin; Cheng-Wen Lin; Hui-Ju Lin; Fu-Tong Liu; Lei Wan. Allyl Isothiocyanate Ameliorates Obesity by Inhibiting Galectin-12. Molecular Nutrition & Food Research 2018, 62, 1700616 .
AMA StyleChia-Wen Lo, Chih-Sheng Chen, Ying-Chi Chen, Yu-An Hsu, Chi-Chun Huang, Ching-Yao Chang, Chao-Jen Lin, Cheng-Wen Lin, Hui-Ju Lin, Fu-Tong Liu, Lei Wan. Allyl Isothiocyanate Ameliorates Obesity by Inhibiting Galectin-12. Molecular Nutrition & Food Research. 2018; 62 (6):1700616.
Chicago/Turabian StyleChia-Wen Lo; Chih-Sheng Chen; Ying-Chi Chen; Yu-An Hsu; Chi-Chun Huang; Ching-Yao Chang; Chao-Jen Lin; Cheng-Wen Lin; Hui-Ju Lin; Fu-Tong Liu; Lei Wan. 2018. "Allyl Isothiocyanate Ameliorates Obesity by Inhibiting Galectin-12." Molecular Nutrition & Food Research 62, no. 6: 1700616.