The aim of the study was the application of high-tech wind tunnel device to identify the changes in the biokinetic parameters of running performed on the specially designed treadmill. The research was carried out in the “Planica Nordic Centre—PNC” in the wind tunnel system, where the AirRunner Assault treadmill, which was equipped with four sensors measuring the vertical and horizontal ground reaction forces, was installed. To obtain biokinetic data, the runners performed the treadmill’s run under conditions of airflow directed at each participant’s back (backwind speeds +3 m/s and +5 m/s) and the chest (headwind speeds −5 m/s and −7 m/s). The runner’s speed was measured via image analysis using a DSLR camera and markers on the belt of the treadmill. Additionally, a high-speed camera synchronised to the force acquisition system was used to analyse the contact phase via comparison of foot placement and time series of the ground reaction forces. The contact phases of the running step were found to be longer than the flight phases, with their duration ranging from 0.15 to 0.20 s and the maximum forces at take-off were found to be greater than when running with the backwind. It should be noted that the application of high-tech devices wind tunnel and treadmill were found to be sufficiently accurate to perform kinetic measurements of running parameters in changing conditions, such as resistance and assistance (facilitating).