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Dr. Mohammad Ansari
Department of Botany, Hindu College Moradabad (Mahatma Jyotiba Phule Rohilkhand University Bareilly)-India

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0 Botany
0 biofilm
0 Honey -natural products
0 Plant physiology and biochemistry
0 Microbiology (MIC, MBC, antimicrobial synergy tests) and laser applications in biomedical sciences

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Journal article
Published: 26 August 2021 in Sustainability
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Lentil (Lens culinaris Medik) is an important component of the human diet due to its high mineral and protein contents. Abiotic stresses, i.e., drought, decreases plant growth and yield. Drought causes the synthesis of reactive oxygen species, which decrease a plant’s starch contents and growth. However, ACC-deaminase (1-aminocyclopropane-1-carboxylate deaminase) producing rhizobacteria can alleviate drought stress by decreasing ethylene levels. On the other hand, caffeic acid (CA) can also positively affect cell expansion and turgor pressure maintenance under drought stress. Therefore, the current study was planned with an aim to assess the effect of CA (0, 20, 50 and 100 ppm) and ACC-deaminase rhizobacteria (Lysinibacillus fusiform, Bacillus amyloliquefaciens) on lentils under drought stress. The combined application of CA and ACC-deaminase containing rhizobacteria significantly improved plant height (55%), number of pods per plant (51%), 1000-grain weight (45%), nitrogen concentration (56%), phosphorus concentration (19%), potassium concentration (21%), chlorophyll (54%), relative water contents RWC (60%) and protein contents (55%). A significant decrease in electrolyte leakage (30%), proline contents (44%), and hydrogen peroxide contents (54%), along with an improvement in cell membrane stability (34% over control) validated the combined use of CA and rhizobacteria. In conclusion, co-application of CA (20 ppm) and ACC-deaminase producing rhizobacteria can significantly improve plant growth and yield for farmers under drought stress. More investigations are suggested at the field level to select the best rhizobacteria and CA level for lentils under drought.

ACS Style

Muhammad Zafar-Ul-Hye; Muhammad Naeem Akbar; Yasir Iftikhar; Mazhar Abbas; Atiqa Zahid; Shah Fahad; Rahul Datta; Muqarrab Ali; Abdallah M. Elgorban; Mohammad Javed Ansari; Subhan Danish. Rhizobacteria Inoculation and Caffeic Acid Alleviated Drought Stress in Lentil Plants. Sustainability 2021, 13, 9603 .

AMA Style

Muhammad Zafar-Ul-Hye, Muhammad Naeem Akbar, Yasir Iftikhar, Mazhar Abbas, Atiqa Zahid, Shah Fahad, Rahul Datta, Muqarrab Ali, Abdallah M. Elgorban, Mohammad Javed Ansari, Subhan Danish. Rhizobacteria Inoculation and Caffeic Acid Alleviated Drought Stress in Lentil Plants. Sustainability. 2021; 13 (17):9603.

Chicago/Turabian Style

Muhammad Zafar-Ul-Hye; Muhammad Naeem Akbar; Yasir Iftikhar; Mazhar Abbas; Atiqa Zahid; Shah Fahad; Rahul Datta; Muqarrab Ali; Abdallah M. Elgorban; Mohammad Javed Ansari; Subhan Danish. 2021. "Rhizobacteria Inoculation and Caffeic Acid Alleviated Drought Stress in Lentil Plants." Sustainability 13, no. 17: 9603.

Journal article
Published: 23 August 2021 in Sustainability
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An experiment was carried out to investigate the effects of different mineral fertilizers on mineral contents in turmeric rhizomes and soil enzyme activities and soil properties under field conditions in Uzbekistan. The present study is the first report on the impact of mineral fertilizers in turmeric rhizomes and soil enzymes and soil properties in Uzbekistan. The experiment was carried out with four treatments: T1—Control, T2—N75P50K50 kg/ha, T3—N125P100K100 kg/ha, and T4—N100P75K75 + B3Zn6Fe6 kg/ha. Turmeric rhizomes and soil samples were collected from field experiments at the Surkhandarya scientific experimental station of the vegetable, melon crops and potato research institute, Surkhandarya, Uzbekistan. The data showed that T3—the NPK (125:100:100 kg/ha) and T4—the NPK + BZnFe (100:75:75:3:6:6 kg/ha) treatments significantly enhanced K content by 27–21%, Ca content by 43–38%, and P content by 54–17% in turmeric rhizomes as compared to control without fertilizer. A maximum of turmeric rhizome microelements content was recorded with T4, which also resulted in improved Fe, Zn, Cu, Cr, and Mo contents in turmeric rhizomes and mineral contents of soil compared to other treatments. This treatment significantly enhanced active P content by 34%, active K content by 25%, total P content by 62%, total K content by 14%, and the activities of soil urease, invertase, catalase, and phosphatase over those in the control. The present study results suggest that the application of NPK + BZnFe (100:75:75:3:6:6 kg/ha) improves macro and micronutrient contents in turmeric rhizomes and activities of soil enzymes and physicochemical properties of soil.

ACS Style

Dilfuza Jabborova; Khurshid Sulaymanov; R. Z. Sayyed; Saad H. Alotaibi; Yuriy Enakiev; Abdulahat Azimov; Zafarjon Jabbarov; Mohammad Javed Ansari; Shah Fahad; Subhan Danish; Rahul Datta. Mineral Fertilizers Improves the Quality of Turmeric and Soil. Sustainability 2021, 13, 9437 .

AMA Style

Dilfuza Jabborova, Khurshid Sulaymanov, R. Z. Sayyed, Saad H. Alotaibi, Yuriy Enakiev, Abdulahat Azimov, Zafarjon Jabbarov, Mohammad Javed Ansari, Shah Fahad, Subhan Danish, Rahul Datta. Mineral Fertilizers Improves the Quality of Turmeric and Soil. Sustainability. 2021; 13 (16):9437.

Chicago/Turabian Style

Dilfuza Jabborova; Khurshid Sulaymanov; R. Z. Sayyed; Saad H. Alotaibi; Yuriy Enakiev; Abdulahat Azimov; Zafarjon Jabbarov; Mohammad Javed Ansari; Shah Fahad; Subhan Danish; Rahul Datta. 2021. "Mineral Fertilizers Improves the Quality of Turmeric and Soil." Sustainability 13, no. 16: 9437.

Journal article
Published: 14 August 2021 in Sustainability
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Biochar, prepared from organic waste materials, can improve the quality of contaminated soil areas. Biochar can be used as an economic centerpiece over other available resources and can properly utilize large amounts of waste. Soil contaminated with cadmium (Cd) is a worldwide problem that poses potential agricultural and human health hazards. Moreover, Cd toxicity causes serious problems for sustainable food production, especially in food crops like barley. High cadmium concentration in soil is phytotoxic and decreases plant growth and ultimately yields. Biochar and ascorbic acid in ameliorating Cd stress are economically compatible and consistent approaches in agriculture. The present study aimed to evaluate biochar’s and foliar-applied ascorbic acid’s influence on some growth and biochemical characteristics of barley (Hordeum vulgare L.) to Cd stress. The soil was supplemented with biochar 2% w/w and 20 mg Cd kg−1. The foliar application of 30 mM ascorbic acid was done on plants. The results revealed that Cd stress decreased chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids. It also increased oxidative stress indicators, i.e., APX, COD, POD, flavonoids, anthocyanin, phenolics, and electrolyte leakage, in barley with Cd-contamination. A significant enhancement in root and shoot length, gas exchange attributes, and chlorophyll contents validated the effectiveness of Bio + Asa treatments over all other treatments under Cd contamination. In conclusion, the sole applications of biochar and Asa in Cd contamination are also effective, but Bio + Asa is a better amendment for Cd stress alleviation in barley plants.

ACS Style

Samia Yaseen; Syeda Fasiha Amjad; Nida Mansoora; Shameem Kausar; Huma Shahid; Saad A. M. Alamri; Sulaiman A. Alrumman; Ebrahem M. Eid; Mohammad Javed Ansari; Subhan Danish; Rahul Datta. Supplemental Effects of Biochar and Foliar Application of Ascorbic Acid on Physio-Biochemical Attributes of Barley (Hordeum vulgare L.) under Cadmium-Contaminated Soil. Sustainability 2021, 13, 9128 .

AMA Style

Samia Yaseen, Syeda Fasiha Amjad, Nida Mansoora, Shameem Kausar, Huma Shahid, Saad A. M. Alamri, Sulaiman A. Alrumman, Ebrahem M. Eid, Mohammad Javed Ansari, Subhan Danish, Rahul Datta. Supplemental Effects of Biochar and Foliar Application of Ascorbic Acid on Physio-Biochemical Attributes of Barley (Hordeum vulgare L.) under Cadmium-Contaminated Soil. Sustainability. 2021; 13 (16):9128.

Chicago/Turabian Style

Samia Yaseen; Syeda Fasiha Amjad; Nida Mansoora; Shameem Kausar; Huma Shahid; Saad A. M. Alamri; Sulaiman A. Alrumman; Ebrahem M. Eid; Mohammad Javed Ansari; Subhan Danish; Rahul Datta. 2021. "Supplemental Effects of Biochar and Foliar Application of Ascorbic Acid on Physio-Biochemical Attributes of Barley (Hordeum vulgare L.) under Cadmium-Contaminated Soil." Sustainability 13, no. 16: 9128.

Research article
Published: 27 July 2021 in PLoS ONE
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Screening for drought tolerance requires precise techniques like phonemics, which is an emerging science aimed at non-destructive methods allowing large-scale screening of genotypes. Large-scale screening complements genomic efforts to identify genes relevant for crop improvement. Thirty maize inbred lines from various sources (exotic and indigenous) maintained at Dryland Agriculture Research Station were used in the current study. In the automated plant transport and imaging systems (LemnaTec Scanalyzer system for large plants), top and side view images were taken of the VIS (visible) and NIR (near infrared) range of the light spectrum to capture phenes. All images were obtained with a thermal imager. All sensors were used to collect images one day after shifting the pots from the greenhouse for 11 days. Image processing was done using pre-processing, segmentation and flowered by features’ extraction. Different surrogate traits such as pixel area, plant aspect ratio, convex hull ratio and calliper length were estimated. A strong association was found between canopy temperature and above ground biomass under stress conditions. Promising lines in different surrogates will be utilized in breeding programmes to develop mapping populations for traits of interest related to drought resilience, in terms of improved tissue water status and mapping of genes/QTLs for drought traits.

ACS Style

Zahoor A. Dar; Showket A. Dar; Jameel A. Khan; Ajaz A. Lone; Sapna Langyan; B. A. Lone; R. H. Kanth; Asif Iqbal; Jagdish Rane; Shabir H. Wani; Saleh Alfarraj; Sulaiman Ali Alharbi; Marian Brestic; Mohammad Javed Ansari. Identification for surrogate drought tolerance in maize inbred lines utilizing high-throughput phenomics approach. PLoS ONE 2021, 16, e0254318 .

AMA Style

Zahoor A. Dar, Showket A. Dar, Jameel A. Khan, Ajaz A. Lone, Sapna Langyan, B. A. Lone, R. H. Kanth, Asif Iqbal, Jagdish Rane, Shabir H. Wani, Saleh Alfarraj, Sulaiman Ali Alharbi, Marian Brestic, Mohammad Javed Ansari. Identification for surrogate drought tolerance in maize inbred lines utilizing high-throughput phenomics approach. PLoS ONE. 2021; 16 (7):e0254318.

Chicago/Turabian Style

Zahoor A. Dar; Showket A. Dar; Jameel A. Khan; Ajaz A. Lone; Sapna Langyan; B. A. Lone; R. H. Kanth; Asif Iqbal; Jagdish Rane; Shabir H. Wani; Saleh Alfarraj; Sulaiman Ali Alharbi; Marian Brestic; Mohammad Javed Ansari. 2021. "Identification for surrogate drought tolerance in maize inbred lines utilizing high-throughput phenomics approach." PLoS ONE 16, no. 7: e0254318.

Journal article
Published: 01 July 2021 in Saudi Journal of Biological Sciences
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Biological control using rhizosphere bacteria, Pseudomonas spp. and Serratia spp. is a prospective alternative technique to overcome plant parasitic nematodes infection. So, the current study was conducted in vitro on five egg-masses, 100 free eggs and 100 infective juveniles (IJs) of Meloidogyne incognita as well as greenhouse treatments on Luffa aegyptiaca L. to evaluate the nematicidal potential of six strains belong to Pseudomonas spp. and Serratia spp. as compared to oxamyl. Results showed that the inhibitory effect and juvenile mortality varied according to bacteria species, strains and exposure time. All the tested bacteria significantly (P ≤ 0.05) inhibited egg hatching and increased juvenile mortality in vitro. After 3 days of treatment, Pseudomonas spp. were more effective against eggs (48.31to 55.15%) and IJs (20.98 to 25.30%) than Serratia spp. (44.55 to 49.75% with eggs) and (19.06 to 21.61% with IJs), respectively. In the pot experiment, Luffa aegyptiaca L. treated with Serratia spp. and Pseudomonas spp. displayed significantly higher (P ≤ 0.05) levels of growth (as indicated by root length, fresh roots weight and fresh shoots weight) compared to control plants and significantly (P ≤ 0.05) suppressed galling (number of galls) and reproduction (as indicated by number of egg-masses on roots and number of eggs and juveniles in pot soil). Meanwhile, among the treated plants, Serratia spp. and Pseudomonas spp. gave the best results in shoot weight of pots infected by eggs of M. incognita than those infected with IJs as compared with positive control. While, oxamyl treatment gave the best results in pots infected by eggs and IJs. The lowest galling (gall index), number of eggs and juveniles in soil was observed in the treatment with mixture of Serratia spp. and Pseudomonas spp. as well as, enhanced growth of sponge gourd more than application each of them alone. Pots treated with oxamyl overwhelmed those treated with mixture of Serratia spp. and Pseudomonas spp.

ACS Style

Elasyed M. Abd El-Aal; Mohamed Shahen; Samy Sayed; Hosny Kesba; Mohammad Javed Ansari; Ramadan M. El-Ashry; Ahmed A.A. Aioub; Ali S.A. Salma; Ahmed M. Eldeeb. In vivo and in vitro management of Meloidogyne incognita (Tylenchida: Heteroderidae) using rhizosphere bacteria, Pseudomonas spp. and Serratia spp. compared with oxamyl. Saudi Journal of Biological Sciences 2021, 28, 4876 -4883.

AMA Style

Elasyed M. Abd El-Aal, Mohamed Shahen, Samy Sayed, Hosny Kesba, Mohammad Javed Ansari, Ramadan M. El-Ashry, Ahmed A.A. Aioub, Ali S.A. Salma, Ahmed M. Eldeeb. In vivo and in vitro management of Meloidogyne incognita (Tylenchida: Heteroderidae) using rhizosphere bacteria, Pseudomonas spp. and Serratia spp. compared with oxamyl. Saudi Journal of Biological Sciences. 2021; 28 (9):4876-4883.

Chicago/Turabian Style

Elasyed M. Abd El-Aal; Mohamed Shahen; Samy Sayed; Hosny Kesba; Mohammad Javed Ansari; Ramadan M. El-Ashry; Ahmed A.A. Aioub; Ali S.A. Salma; Ahmed M. Eldeeb. 2021. "In vivo and in vitro management of Meloidogyne incognita (Tylenchida: Heteroderidae) using rhizosphere bacteria, Pseudomonas spp. and Serratia spp. compared with oxamyl." Saudi Journal of Biological Sciences 28, no. 9: 4876-4883.

Journal article
Published: 29 June 2021 in Saudi Journal of Biological Sciences
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An experiment was conducted at Punjab Agricultural University, Ludhiana during 2014–15 and 2015–16, keeping four sowing dates {25th Oct (D1), 10th Nov (D2), 25th Nov (D3) and 10th Dec (D4)} in main plots and five irrigation schedules {irrigation at 15 (FC15), 25 (FC25), 35 (FC35) and 45 (FC45) % depletion of soil moisture from field capacity (FC) and a conventional practice} in sub plots. The objective of the study was to evaluate the performance of CERES-Wheat model for simulating yield and water use under varying planting and soil moisture regimes. The simulated and observed grain yield was higher in D1, with irrigation applied at FC15 as compared to all other sowing date and irrigation regime combinations. Simulated grain yield decreased by 19% with delay in sowing from 25th October to 10th December because of 8% reduction in simulated crop evapotranspiration. Simulated evapotranspiration decreased by 16%, wheat grain yield by 23% and water productivity by 15% in drip irrigation at 45% depletion from field capacity as compared to drip irrigation at 15% of field capacity. It was further revealed that the model performed well in simulating the phenology, water use and yield of wheat.

ACS Style

Eajaz Ahmad Dar; Ajmer Singh Brar; Showket A. Dar; Bandar S. Aljuaid; Ahmed M. El-Shehawi; Rizwan Rashid; Zahoor A. Shah; Abrar Yousuf; Mohammad Amin Bhat; Mushtaq Ahmed; Fayaz Ahmad Bahar; Hesham El Enshasy; Marian Brestic; Maria Barboricova; Marek Zivcak; Shahid Farooq; Mohammad Javed Ansari. Quantitative response of wheat to sowing dates and irrigation regimes using CERES-Wheat model. Saudi Journal of Biological Sciences 2021, 1 .

AMA Style

Eajaz Ahmad Dar, Ajmer Singh Brar, Showket A. Dar, Bandar S. Aljuaid, Ahmed M. El-Shehawi, Rizwan Rashid, Zahoor A. Shah, Abrar Yousuf, Mohammad Amin Bhat, Mushtaq Ahmed, Fayaz Ahmad Bahar, Hesham El Enshasy, Marian Brestic, Maria Barboricova, Marek Zivcak, Shahid Farooq, Mohammad Javed Ansari. Quantitative response of wheat to sowing dates and irrigation regimes using CERES-Wheat model. Saudi Journal of Biological Sciences. 2021; ():1.

Chicago/Turabian Style

Eajaz Ahmad Dar; Ajmer Singh Brar; Showket A. Dar; Bandar S. Aljuaid; Ahmed M. El-Shehawi; Rizwan Rashid; Zahoor A. Shah; Abrar Yousuf; Mohammad Amin Bhat; Mushtaq Ahmed; Fayaz Ahmad Bahar; Hesham El Enshasy; Marian Brestic; Maria Barboricova; Marek Zivcak; Shahid Farooq; Mohammad Javed Ansari. 2021. "Quantitative response of wheat to sowing dates and irrigation regimes using CERES-Wheat model." Saudi Journal of Biological Sciences , no. : 1.

Research article
Published: 23 June 2021 in PLOS ONE
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Zinc (Zn) is an important micronutrient for crop plants and essential for human health. The Zn-deficiency is an important malnutrition problem known globally. Biofortified foods could overcome Zn deficiency in humans. Mungbean [Vigna radiata (L.) Wilczek] is an important, pulse crop frequently grown in arid and semi-arid regions of the world. Mungbean could provide essential micronutrients, including Zn to humans. Therefore, it is very important to investigate the impact of Zn fertilization on the yield and grain biofortification of mungbean. Twelve mungbean genotypes (i.e., NM-28, NM-2011, NM-13-1, NM-2006, NM-51, NM-54, NM-19-19, NM-92, NM-121-25, NM-20-21, 7006, 7008) were assessed for their genetic diversity followed by Zn-biofortification, growth and yield under control (0 kg ha-1) and Zn-fertilized (10 kg ha-1) conditions. Data relating to allometric traits, yield components, grain yield and grain Zn contents were recorded. Zinc fertilization improved entire allometric and yield-related traits. Grain yield of different genotypes ranged from 439 to 904 kg ha-1 under control and 536 to 1462 kg ha-1 under Zn-fertilization. Zinc concentration in the grains varied from 15.50 to 45.60 mg kg-1 under control and 18.53 to 64.23 mg kg-1 under Zn-fertilized conditions. The tested genotypes differed in their Zn-biofortification potential. The highest and the lowest grain Zn contents were noted for genotypes NM-28 and NM-121-25, respectively. Significant variation in yield and Zn-biofortification indicated the potential for improvement in mungbean yield and grain Zn-biofortification. The genotypes NM-28 and NM-2006 could be used in breeding programs for improvement in grain Zn concentration due to their high Zn uptake potential. Nonetheless, all available genotypes in the country should be screened for their Zn-biofortification potential.

ACS Style

Muhammad Umar Haider; Mubshar Hussain; Muhammad Farooq; Sami Ul-Allah; Mohammad Javed Ansari; Mona S. Alwahibi; Shahid Farooq. Zinc biofortification potential of diverse mungbean [Vigna radiata (L.) Wilczek] genotypes under field conditions. PLOS ONE 2021, 16, e0253085 .

AMA Style

Muhammad Umar Haider, Mubshar Hussain, Muhammad Farooq, Sami Ul-Allah, Mohammad Javed Ansari, Mona S. Alwahibi, Shahid Farooq. Zinc biofortification potential of diverse mungbean [Vigna radiata (L.) Wilczek] genotypes under field conditions. PLOS ONE. 2021; 16 (6):e0253085.

Chicago/Turabian Style

Muhammad Umar Haider; Mubshar Hussain; Muhammad Farooq; Sami Ul-Allah; Mohammad Javed Ansari; Mona S. Alwahibi; Shahid Farooq. 2021. "Zinc biofortification potential of diverse mungbean [Vigna radiata (L.) Wilczek] genotypes under field conditions." PLOS ONE 16, no. 6: e0253085.

Research article
Published: 22 June 2021 in PLOS ONE
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Cytokinins (CKs) plays a key role in plant adaptation over a range of different stress conditions. Here, we analyze the effects of a cytokinin (i.e., kinetin, KN) on the growth, photosynthesis (rate of O2 evolution), PS II photochemistry and AsA–GSH cycle in Trigonella seedlings grown under cadmium (Cd) stress. Trigonella seeds were sown in soil amended with 0, 3 and 9 mg Cd kg-1 soil, and after 15 days resultant seedlings were sprayed with three doses of KN, i.e.,10 μM (low, KNL), 50 μM (medium, KNM) and 100 μM (high, KNH); subsequent experiments were performed after 15 days of KN application, i.e., 30 days after sowing. Cadmium toxicity induced oxidative damage as shown by decreased seedling growth and photosynthetic pigment production (Chl a, Chl b and Car), rates of O2-evolution, and photochemistry of PS II of Trigonella seedlings, all accompanied by an increase in H2O2 accumulation. Supplementation with doses of KN at KNL and KNM significantly improved the growth and photosynthetic activity by reducing H2O2 accumulation through the up-regulation AsA–GSH cycle. Notably, KNL and KNM doses stimulated the rate of enzyme activities of APX, GR and DHAR, involved in the AsA–GSH cycle thereby efficiently regulates the level of AsA and GSH in Trigonella grown under Cd stress. The study concludes that KN can mitigate the damaging effects of Cd stress on plant growth by maintaining the redox status (>ratios: AsA/DHA and GSH/GSSG) of cells through the regulation of AsA-GSH cycle at 10 and 50 μM KN under Cd stress conditions. At 100 μM KN, the down-regulation of AsA-GSH cycle did not support the growth and PS II activity of the test seedlings.

ACS Style

Gausiya Bashri; Shikha Singh; Sheo Mohan Prasad; Mohammad Javed Ansari; Salma Usmani; Saleh Alfarraj; Sulaiman Ali Alharbi; Marian Brestic. Kinetin mitigates Cd-induced damagesto growth, photosynthesis and PS II photochemistry of Trigonella seedlings by up-regulating ascorbate-glutathione cycle. PLOS ONE 2021, 16, e0249230 .

AMA Style

Gausiya Bashri, Shikha Singh, Sheo Mohan Prasad, Mohammad Javed Ansari, Salma Usmani, Saleh Alfarraj, Sulaiman Ali Alharbi, Marian Brestic. Kinetin mitigates Cd-induced damagesto growth, photosynthesis and PS II photochemistry of Trigonella seedlings by up-regulating ascorbate-glutathione cycle. PLOS ONE. 2021; 16 (6):e0249230.

Chicago/Turabian Style

Gausiya Bashri; Shikha Singh; Sheo Mohan Prasad; Mohammad Javed Ansari; Salma Usmani; Saleh Alfarraj; Sulaiman Ali Alharbi; Marian Brestic. 2021. "Kinetin mitigates Cd-induced damagesto growth, photosynthesis and PS II photochemistry of Trigonella seedlings by up-regulating ascorbate-glutathione cycle." PLOS ONE 16, no. 6: e0249230.

Journal article
Published: 11 June 2021 in Journal of King Saud University - Science
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Nanoparticles nowadays are an effective control agent against most phytopathogens. However, there are many reports on their antimicrobial activity and little as a nematicide. In this study, green biosynthesis of silver nanoparticles (Ag NPs) using Acalypha wilkesiana aqueous leaf extract was achieved. X-ray diffraction patterns confirmed the crystalline structure with face-centred cubic (fcc), where is the size was nearly 20 nm. SEM images of the Ag NPs show the spherical shape and in the range from 10 to 30 nm. Various functional groups, capping, and stability agents were identified using Fourier transmission infrared spectroscopy (FTIR). The nematicidal activity of biosynthesized Ag NPs conc. 25, 50, and 100 µg/mL were evaluated in vitro against root-knot nematode (Meloidogyne incognita), egg hatching (6 days after) and movement after 24 and 48 h. The Ag NPs (100 µg/ml) application after 48 h was the most effective treatment that showed 53.3% of nematode mortality. Overall, the efficiency of bio-Ag NPs reduced the nematode activity, mortality, egg hatching, and movement of larvae. To our knowledge, this is the first report of nematicidal action of biosynthesized Ag NPs using A. wilkesiana aqueous extract, and it could be recommended to manage the plant-parasitic nematode as it is simple, stable, cost-effective and keep the environment safe.

ACS Style

Ahmed A. Heflish; Amira E. Hanfy; Mohammad Javed Ansari; Eldessoky S. Dessoky; Attia O. Attia; Moustafa M. Elshaer; Mohamed K. Gaber; Ahmed Kordy; Ahmed S. Doma; Ahmed Abdelkhalek; Said I. Behiry. Green Biosynthesized Silver Nanoparticles Mediated by Acalypha wilkesiana Extract control root-knot nematode. Journal of King Saud University - Science 2021, 33, 101516 .

AMA Style

Ahmed A. Heflish, Amira E. Hanfy, Mohammad Javed Ansari, Eldessoky S. Dessoky, Attia O. Attia, Moustafa M. Elshaer, Mohamed K. Gaber, Ahmed Kordy, Ahmed S. Doma, Ahmed Abdelkhalek, Said I. Behiry. Green Biosynthesized Silver Nanoparticles Mediated by Acalypha wilkesiana Extract control root-knot nematode. Journal of King Saud University - Science. 2021; 33 (6):101516.

Chicago/Turabian Style

Ahmed A. Heflish; Amira E. Hanfy; Mohammad Javed Ansari; Eldessoky S. Dessoky; Attia O. Attia; Moustafa M. Elshaer; Mohamed K. Gaber; Ahmed Kordy; Ahmed S. Doma; Ahmed Abdelkhalek; Said I. Behiry. 2021. "Green Biosynthesized Silver Nanoparticles Mediated by Acalypha wilkesiana Extract control root-knot nematode." Journal of King Saud University - Science 33, no. 6: 101516.

Research article
Published: 10 June 2021 in PLOS ONE
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Peanut (Arachis hypogea L.) is an important nut crop extensively grown in rainfed regions of Pakistan. The crop requires low inputs; thus, could grow successfully under diverse environmental conditions. Due to pegging ability, peanut grows aggressively in sandy and sandy-loam soils. However, it has not introduced to Thal region of southern Punjab, Pakistan. A two-year field experiment was conducted to optimize sowing dates for two peanut genotypes (‘BARI-2016’ and ‘NO-334’) in Thal region (Layyah). Similarly, a yield trial was conducted at Chakwal where both genotypes are extensively grown. Five sowing dates (10th April, 1st May, 20th May, 10th June and 30th June) were included in the study. The highest seed yield was obtained with early sown crop (10th April) during both years. Pod formation reduced with increasing atmospheric temperature and no pods were formed on the plants sown on 30th June. Decreased pod formation seemed a major reason for low yield in late-sown crop. The highest yield was observed for the crop sown on 10th April, which was decreased by 40% for the crop sown on 1st May. Genotype ‘BARI-2016’ performed better for seed yield at both locations compared with ‘NO-334’. The results suggested that genotype ‘BARI-2016’ is more adaptive to arid and semi-arid condition under rainfed or irrigated conditions. Sowing peanut at optimum time would increase seed yield in arid and semi-arid regions. Nonetheless, ‘BARI-2016’ can be grown under rainfed and irrigated conditions successfully.

ACS Style

Muhammad Ijaz; Ahmad Nawaz; Sami Ul-Allah; Ahmad Sher; Abdul Sattar; Muhammad Sarwar; Ijaz Hussain; Anees Ur Rehman; Muhammad Ashfaq Wahid; Mohammad Javed Ansari; Kamel Hessini. Optimizing sowing date for peanut genotypes in arid and semi-arid subtropical regions. PLOS ONE 2021, 16, e0252393 .

AMA Style

Muhammad Ijaz, Ahmad Nawaz, Sami Ul-Allah, Ahmad Sher, Abdul Sattar, Muhammad Sarwar, Ijaz Hussain, Anees Ur Rehman, Muhammad Ashfaq Wahid, Mohammad Javed Ansari, Kamel Hessini. Optimizing sowing date for peanut genotypes in arid and semi-arid subtropical regions. PLOS ONE. 2021; 16 (6):e0252393.

Chicago/Turabian Style

Muhammad Ijaz; Ahmad Nawaz; Sami Ul-Allah; Ahmad Sher; Abdul Sattar; Muhammad Sarwar; Ijaz Hussain; Anees Ur Rehman; Muhammad Ashfaq Wahid; Mohammad Javed Ansari; Kamel Hessini. 2021. "Optimizing sowing date for peanut genotypes in arid and semi-arid subtropical regions." PLOS ONE 16, no. 6: e0252393.

Research article
Published: 13 May 2021 in PLOS ONE
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The buttonwood (Conocarpus erectus L.) is a mangrove shrub belonging to Combretaceae family. It mostly grows on the shorelines of tropical and subtropical regions in the world. It was introduced to Lasbela University of Water, Agriculture & Marine Sciences (LUWMS), Uthal, Baluchistan as an ornamental plant as it grows well under harsh, temperate and saline conditions. During a routine survey, typical leaf spot symptoms were observed on the leaves of buttonwood plants. A disease severity scale for alternaria leaf spot of buttonwood was developed for the first time through this study. Disease severity according to the scale was 38.97%. The microscopic characterizations was accomplished for the identification of Alternaria alternata and Koch’s postulates were employed to determine the pathogenicity. For molecular identification, 650 bp internal transcribed spacer (ITS) regions (ITS1, 5.8s and ITS2) were amplified from three representative isolates (LUAWMS1, LUAWMS2 and LUAWMS3) through polymerase chain reaction (PCR). The nucleotide sequences from ITS regions of the isolates were submitted to NCBI with GenBank accession numbers MW585375, MW585376 and MW585377, respectively. The phylogenetic tree of 22 A. alternata isolates was computed and representative isolates exhibited 99.98% genetic similarity with mangroves ecosystem isolates. This study reports the incidence of alternaria leaf spot of buttonwood at LUWMS for the first time. It is suspected that the disease may spread further. Therefore, effective management strategies should be opted to halt the further spread of the disease.

ACS Style

Muhammad Fahim Abbas; Muhammad Rafiq; Abdullah M. Al-Sadi; Saleh Alfarraj; Sulaiman Ali Alharbi; Muhammad Arif; Mohammad Javed Ansari. Molecular characterization of leaf spot caused by Alternaria alternata on buttonwood (Conocarpus erectus L.) and determination of pathogenicity by a novel disease rating scale. PLOS ONE 2021, 16, e0251471 .

AMA Style

Muhammad Fahim Abbas, Muhammad Rafiq, Abdullah M. Al-Sadi, Saleh Alfarraj, Sulaiman Ali Alharbi, Muhammad Arif, Mohammad Javed Ansari. Molecular characterization of leaf spot caused by Alternaria alternata on buttonwood (Conocarpus erectus L.) and determination of pathogenicity by a novel disease rating scale. PLOS ONE. 2021; 16 (5):e0251471.

Chicago/Turabian Style

Muhammad Fahim Abbas; Muhammad Rafiq; Abdullah M. Al-Sadi; Saleh Alfarraj; Sulaiman Ali Alharbi; Muhammad Arif; Mohammad Javed Ansari. 2021. "Molecular characterization of leaf spot caused by Alternaria alternata on buttonwood (Conocarpus erectus L.) and determination of pathogenicity by a novel disease rating scale." PLOS ONE 16, no. 5: e0251471.

Research article
Published: 13 May 2021 in PLOS ONE
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Vehicular emissions cause heavy metal pollution and exert negative impacts on environment and roadside vegetation. Wild plants growing along roadsides are capable of absorbing considerable amounts of heavy metals; thus, could be helpful in reducing heavy metal pollution. Therefore, current study inferred heavy metal absorbance capacity of some wild plant species growing along roadside. Four different wild plant species, i.e., Acacia nilotica L., Calotropis procera L., Ricinus communis L., and Ziziphus mauritiana L. were selected for the study. Leaf samples of these species were collected from four different sites, i.e., Control, New Lahore, Nawababad and Fatehabad. Leaf samples were analyzed to determine Pb2+, Zn2+, Ni2+, Mn2+ and Fe3+ accumulation. The A. nilotica, Z. mauritiana and C. procera accumulated significant amount of Pb at New Lahore site. Similarly, R. communis and A. nilotica accumulated higher amounts of Mn, Zn and Fe at Nawababad and New Lahore sites compared to the rest of the species. Nonetheless, Z. mauritiana accumulated higher amounts of Ni at all sites compared with the other species included in the study. Soil surface contributed towards the uptake of heavy metals in leaves; therefore, wild plant species should be grown near the roadsides to control heavy metals pollution. Results revealed that wild plants growing along roadsides accumulate significant amounts of heavy metals. Therefore, these species could be used to halt the vehicular pollution along roadsides and other polluted areas.

ACS Style

Rubina Altaf; Sikandar Altaf; Mumtaz Hussain; Rahmat Ullah Shah; Rehmat Ullah; Muhammad Ihsan Ullah; Abdul Rauf; Mohammad Javed Ansari; Sulaiman Ali Alharbi; Saleh Alfarraj; Rahul Datta. Heavy metal accumulation by roadside vegetation and implications for pollution control. PLOS ONE 2021, 16, e0249147 .

AMA Style

Rubina Altaf, Sikandar Altaf, Mumtaz Hussain, Rahmat Ullah Shah, Rehmat Ullah, Muhammad Ihsan Ullah, Abdul Rauf, Mohammad Javed Ansari, Sulaiman Ali Alharbi, Saleh Alfarraj, Rahul Datta. Heavy metal accumulation by roadside vegetation and implications for pollution control. PLOS ONE. 2021; 16 (5):e0249147.

Chicago/Turabian Style

Rubina Altaf; Sikandar Altaf; Mumtaz Hussain; Rahmat Ullah Shah; Rehmat Ullah; Muhammad Ihsan Ullah; Abdul Rauf; Mohammad Javed Ansari; Sulaiman Ali Alharbi; Saleh Alfarraj; Rahul Datta. 2021. "Heavy metal accumulation by roadside vegetation and implications for pollution control." PLOS ONE 16, no. 5: e0249147.

Research article
Published: 26 March 2021 in PLOS ONE
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Segregation distortion (SD) is a genetic mechanism commonly found in segregating or stable populations. The principle behind this puzzles many researchers. The F2generation developed from wildGossypium darwiniiandG.hirsutumCCRI12 species was used to investigate the possible transcription factors within the segregation distortion regions (SDRs). The 384 out of 2763 markers were distorted in 29 SDRs on 18 chromosomes. Good collinearity was observed among genetic and physical maps ofG.hirsutumandG.barbadensesyntenic blocks. Total 568 genes were identified from SDRs of 18 chromosomes. Out of these genes, 128 belonged to three top-ranked salt-tolerant gene families. The DUF597 contained 8 uncharacterized genes linked to Pkinase (PF00069) gene family in the phylogenetic tree, while 15 uncharacterized genes clustered with the zinc finger gene family. Two hundred thirty four miRNAs targeted numerous genes, including ghr-miR156, ghr-miR399 and ghr-miR482, while others targeted top-ranked stress-responsive transcription factors. Moreover, these genes were involved in the regulation of numerous stress-responsive cis-regulatory elements. The RNA sequence data of fifteen upregulated genes were verified through the RT-qPCR. The expression profiles of two highly upregulated genes (Gh_D01G2015andGh_A01G1773) in salt-tolerantG.darwiniishowed antagonistic expression inG.hirsutum. The results indicated that salt-tolerant genes have been possibly transferred from the wildG.darwiniispecies. A detailed functional analysis of these genes can be carried out which might be helpful in the future for gene cloning, transformation, gene editing and the development of salt-resistant cotton varieties.

ACS Style

Muhammad Shehzad; Zhongli Zhou; Allah Ditta; Majid Khan; Xiaoyan Cai; Yanchao Xu; Amir Maqbool; Ahlam Khalofah; Muhammad Shaban; Muhammad Naeem; Mohammad Javed Ansari; Kunbo Wang; Fang Liu. Identification and characterization of genes related to salt stress tolerance within segregation distortion regions of genetic map in F2 population of upland cotton. PLOS ONE 2021, 16, e0247593 .

AMA Style

Muhammad Shehzad, Zhongli Zhou, Allah Ditta, Majid Khan, Xiaoyan Cai, Yanchao Xu, Amir Maqbool, Ahlam Khalofah, Muhammad Shaban, Muhammad Naeem, Mohammad Javed Ansari, Kunbo Wang, Fang Liu. Identification and characterization of genes related to salt stress tolerance within segregation distortion regions of genetic map in F2 population of upland cotton. PLOS ONE. 2021; 16 (3):e0247593.

Chicago/Turabian Style

Muhammad Shehzad; Zhongli Zhou; Allah Ditta; Majid Khan; Xiaoyan Cai; Yanchao Xu; Amir Maqbool; Ahlam Khalofah; Muhammad Shaban; Muhammad Naeem; Mohammad Javed Ansari; Kunbo Wang; Fang Liu. 2021. "Identification and characterization of genes related to salt stress tolerance within segregation distortion regions of genetic map in F2 population of upland cotton." PLOS ONE 16, no. 3: e0247593.

Journal article
Published: 24 March 2021 in Insects
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Some beetle species can attack honeybee colonies, causing severe damage to beekeeping. These pests include Oplostomus fuligineus, which is also known as the Large Hive Beetle (LHB). This beetle is native to Sub-Saharan Africa and has recently also been recorded in some parts of North Africa. It feeds mainly on young bee larvae and stored food within the colonies, causing severe damage to weak colonies. The present work sheds light on the current and future distribution (from 2050 to 2070) of this beetle in Africa and South Europe using species distribution modeling. Maxent was used to model the invasion of LHB. The Shared Socioeconomic Pathways (SSPs) 126 and 585 were used to model the future distribution of LHB. The Maxent models showed satisfactory results with a high Area Under Curve (AUC) value (0.85 ± 0.02). Furthermore, the True Skill Statistics (TSS) value was equal to 0.87. The current and future maps showed a high risk of invasion because of temperature variation in most of the parts of North Africa and South Europe. The maps also predicted the future invasion of LHB into other countries, mainly through southern Europe. These predictive risk maps will help quarantine authorities in highly relevant countries to prevent the expansion of this pest outside of its natural range.

ACS Style

Hossam Abou-Shaara; Sara Alashaal; Eslam Hosni; Mohamed Nasser; Mohammad Ansari; Sulaiman Alharbi. Modeling the Invasion of the Large Hive Beetle, Oplostomusfuligineus, into North Africa and South Europe under a Changing Climate. Insects 2021, 12, 275 .

AMA Style

Hossam Abou-Shaara, Sara Alashaal, Eslam Hosni, Mohamed Nasser, Mohammad Ansari, Sulaiman Alharbi. Modeling the Invasion of the Large Hive Beetle, Oplostomusfuligineus, into North Africa and South Europe under a Changing Climate. Insects. 2021; 12 (4):275.

Chicago/Turabian Style

Hossam Abou-Shaara; Sara Alashaal; Eslam Hosni; Mohamed Nasser; Mohammad Ansari; Sulaiman Alharbi. 2021. "Modeling the Invasion of the Large Hive Beetle, Oplostomusfuligineus, into North Africa and South Europe under a Changing Climate." Insects 12, no. 4: 275.

Journal article
Published: 23 March 2021 in Journal of King Saud University - Science
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The present study assessed physico-chemical, techno-functional, thermal and textural characteristics of propolis samples (n = 30) collected from four Northern zones of India. The propolis samples were analyzed according to the Association of Official Analytical Chemists (AOAC) official protocols for their ash, moisture, and protein content. Soxtech, Fiber tech, Hunter LAB Colorimeter, TA.XT2i Texture Analyzer, Thermo gravimetric Analyzer, Discovery DSC 25 have been used to assess the crude fat, fibre content, colour, texture and thermal properties, respectively. Physico-chemical properties of propolis differed significantly (p < 0.05) and reportedthe highest balsam content (47.66%), protein (9.41%) and wax content (22.9%) in Punjab propolis (PP) where as the moisture content and water activity ranged from 4.89 to 7.37% and 0.73–0.81, respectively in propolis samples. Regarding techno-functional properties, PP had highest oil holding capacity (1.09–1.90 g/g), foaming capacity (11.08–17.03%), foam stability (67.3–75.43%), emulsifying activity (4.18–7.62%), and emulsion stability (3.24–4.77%). The positive a* and b* values of colour in this investigation indicated that the samples from all four zones lied in reddish yellowish areas of LAB colour space and relatively dull or gray as indicated by chroma values. Results of texture profile analysis demonstrated that all propolis samples were hard, cohesive, chewy and showed resilience. Differential scanning calorimetry predicted semi-crystalline behaviours of all samples. Three principal components, accounting for 98.95% variation were abstracted from fourteen physiochemical, seven techno-functional, seven textural and four thermal variables. Further, hierarchical cluster analysis successfully classified the propolis samples into four zones.

ACS Style

Kirty Pant; Mamta Thakur; H.K. Chopra; Vikas Nanda; Mohammad Javed Ansari; Giacomo Pietramellara; Shamina Imran Pathan; Sulaiman Ali Alharbi; Hesham S. Almoallim; Rahul Datta. Characterization and discrimination of Indian propolis based on physico-chemical, techno-functional, thermal and textural properties: A multivariate approach. Journal of King Saud University - Science 2021, 33, 101405 .

AMA Style

Kirty Pant, Mamta Thakur, H.K. Chopra, Vikas Nanda, Mohammad Javed Ansari, Giacomo Pietramellara, Shamina Imran Pathan, Sulaiman Ali Alharbi, Hesham S. Almoallim, Rahul Datta. Characterization and discrimination of Indian propolis based on physico-chemical, techno-functional, thermal and textural properties: A multivariate approach. Journal of King Saud University - Science. 2021; 33 (4):101405.

Chicago/Turabian Style

Kirty Pant; Mamta Thakur; H.K. Chopra; Vikas Nanda; Mohammad Javed Ansari; Giacomo Pietramellara; Shamina Imran Pathan; Sulaiman Ali Alharbi; Hesham S. Almoallim; Rahul Datta. 2021. "Characterization and discrimination of Indian propolis based on physico-chemical, techno-functional, thermal and textural properties: A multivariate approach." Journal of King Saud University - Science 33, no. 4: 101405.

Journal article
Published: 23 March 2021 in Journal of King Saud University - Science
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A field trial was carried out in Kashmir valley to determine the persistence of fenazaquin 10EC (Magister) in Red Delicious variety of apple at recommended (0.004%) and double the recommended (0.008%) application rates. The spray was conducted one month prior to harvest. The plants treated with simple tap water were treated as control. Samples were collected at 0, 3, 7, 10, 15, 20 days and harvest. The procedure followed for extraction and cleanup was that of Luke et al. (1985) modified by Sharma (2007) and the final analysis was carried out on a Varian 450 (Walnut Creek, CA, USA) gas chromatograph (GLC) equipped with Thermionic Specific detector (TSD). After computation of data, the initial deposit was recorded as 3.18 ± 0.03 μg g − 1 and 6.98 ± 0.08 μg g − 1 at two concentrations, respectively. Fenazaquin (0.004%) dissipated to 96.91 per cent in 20 days after application and was not detectable beyond this period. Fenazaquin (0.008%) however, persisted upto 30 days recording 95.84 per cent dissipation at that time. The progressive dissipation of fenazaquin (0.004%) and fenazaquin (0.008%) residues down to their tolerance limits suggested a waiting period of 18.55 and 30.49 days with a half-life period of 3.62 and 4.12 days, respectively. The terminal residue of fenazaquin at the lower rate was below maximum residue limit (MRL) set by European Union, however above MRL at the higher rate.

ACS Style

Munazah Yaqoob; F.A. Zaki; Malik Mukhtar; Sheikh Bilal Ahmed; Muhammad Azhar Khan; Liyaqat Ayoub; Umar Bin-Farook; Syed Ishtiaq Anjum; Mohammad Javed Ansari; Hesham S. Almoallim; Sulaiman Ali Alharbi; Peter Ondrisik. Residual fate of fenazaquin (10EC) in apple fruit and soil. Journal of King Saud University - Science 2021, 33, 101415 .

AMA Style

Munazah Yaqoob, F.A. Zaki, Malik Mukhtar, Sheikh Bilal Ahmed, Muhammad Azhar Khan, Liyaqat Ayoub, Umar Bin-Farook, Syed Ishtiaq Anjum, Mohammad Javed Ansari, Hesham S. Almoallim, Sulaiman Ali Alharbi, Peter Ondrisik. Residual fate of fenazaquin (10EC) in apple fruit and soil. Journal of King Saud University - Science. 2021; 33 (4):101415.

Chicago/Turabian Style

Munazah Yaqoob; F.A. Zaki; Malik Mukhtar; Sheikh Bilal Ahmed; Muhammad Azhar Khan; Liyaqat Ayoub; Umar Bin-Farook; Syed Ishtiaq Anjum; Mohammad Javed Ansari; Hesham S. Almoallim; Sulaiman Ali Alharbi; Peter Ondrisik. 2021. "Residual fate of fenazaquin (10EC) in apple fruit and soil." Journal of King Saud University - Science 33, no. 4: 101415.

Research article
Published: 25 February 2021 in PLOS ONE
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Rice (Oryza sativa L.) feeds to two-third of the global population by serving as staple food. It is the main export commodity of several countries; thus, contributes towards foreign exchange earnings. Unfortunately, average global rice yield is far below than its genetic potential. Low nitrogen (N) use efficiency (NUE) is among the major reasons for low average yield. Current study evaluated the impact of nitrogen fertilizer application methods (conventional and deep placement) on growth, yield-related traits, chlorophyll contents, photosynthesis rate, agronomic N-use efficiency (ANUE), partial factors productivity of applied N (PFP) and economic returns of two different transplanted rice varieties (Basmati-515 and Super-Basmati). Fertilizer application methods significantly affected allometry, yield-related traits, chlorophyll contents, photosynthesis rate, ANUE, PFP and economic returns. Deep placement of N-fertilizer (DPNF) observed better allometric traits, high chlorophyll contents, photosynthesis rate, ANUE, PFP, yield attributes and economic returns compared to conventional application of N-fertilizer (CANF). Similarly, Basmati-515 had better allometric and yield-related traits, chlorophyll contents, photosynthesis rate, ANUE, PFP and economic returns than Super-Basmati. Regarding interactions among N-fertilizer application methods and rice varieties, Basmati-515 with DPNF resulted in higher chlorophyll contents, photosynthesis rate, ANUE, PFP, allometric and yield related traits and economic returns than CANF. The lowest values of these traits were observed for Super-Basmati with no application of N-fertilizer. Both varieties had better yield and economic returns with DPNF compared to CANF. It is concluded that DPNF improved yield, ANUE and economic returns; therefore, should be opted to improve productivity of transplanted fine rice. Nonetheless, lower nitrogen doses need to be tested for DPNF to infer whether it could lower N use in rice crop.

ACS Style

Ahlam Khalofah; Muhammad Ifnan Khan; Muhammad Arif; Ansar Hussain; Rehmat Ullah; Muhammad Irfan; Shahzadi Mahpara; Rahmat Ullah Shah; Mohammad Javed Ansari; Antonin Kintl; Martin Brtnicky; Subhan Danish; Rahul Datta. Deep placement of nitrogen fertilizer improves yield, nitrogen use efficiency and economic returns of transplanted fine rice. PLOS ONE 2021, 16, e0247529 .

AMA Style

Ahlam Khalofah, Muhammad Ifnan Khan, Muhammad Arif, Ansar Hussain, Rehmat Ullah, Muhammad Irfan, Shahzadi Mahpara, Rahmat Ullah Shah, Mohammad Javed Ansari, Antonin Kintl, Martin Brtnicky, Subhan Danish, Rahul Datta. Deep placement of nitrogen fertilizer improves yield, nitrogen use efficiency and economic returns of transplanted fine rice. PLOS ONE. 2021; 16 (2):e0247529.

Chicago/Turabian Style

Ahlam Khalofah; Muhammad Ifnan Khan; Muhammad Arif; Ansar Hussain; Rehmat Ullah; Muhammad Irfan; Shahzadi Mahpara; Rahmat Ullah Shah; Mohammad Javed Ansari; Antonin Kintl; Martin Brtnicky; Subhan Danish; Rahul Datta. 2021. "Deep placement of nitrogen fertilizer improves yield, nitrogen use efficiency and economic returns of transplanted fine rice." PLOS ONE 16, no. 2: e0247529.

Research article
Published: 28 December 2020 in PLoS ONE
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Bee pollen is a natural product that has valuable nutritional and medicinal characteristics and has recently garnered increasing attention in the food industry due to its nutritive value. Here, we harvested pollen loads from the Al-Ahsa oasis in eastern Saudi Arabia during spring, summer, autumn, and winter in 2018/2019 to compare the nutritional value of bee pollen protein with the amino acid requirements of honeybees and adult humans. Based on the nutritional value of bee pollen protein, the optimal season for harvesting bee pollen was determined. The composition of the bee pollen showed the highest contents of crude protein, total amino acids, leucine, glutamic acid, valine, isoleucine, threonine, and glycine in samples collected in spring. The highest contents of lysine, phenylalanine, threonine, tryptophan, arginine, tyrosine, and cysteine were observed in samples collected in winter. The highest contents of histidine, methionine, and serine were in samples collected in autumn. Moreover, the highest levels of aspartic acid, proline, and alanine were in samples collected in summer. Leucine, valine, lysine, histidine, threonine, and phenylalanine (except in autumn bee pollen) contents in pollen from all four seasons were above the requirements of honeybees. Leucine, valine, histidine, isoleucine (except in autumn bee pollen), lysine (except in spring and summer bee pollen), and threonine (except in winter and spring bee pollen) in all tested samples were above the requirements of adult humans. In comparison with the minimal amino acid requirements of adult humans and honeybees, the 1stlimiting amino acid in bee pollen collected during the different seasons was methionine. Bee pollen collected during spring (March–May) and winter (December–February) can be considered a nutritive food source for adult humans and honeybees.

ACS Style

Saad N. Al-Kahtani; El-Kazafy Taha; Khalid Ali Khan; Mohammad Javed Ansari; Soha A. Farag; Dalia M. B. Shawer; El-Said Mohamed Elnabawy. Effect of harvest season on the nutritional value of bee pollen protein. PLoS ONE 2020, 15, e0241393 .

AMA Style

Saad N. Al-Kahtani, El-Kazafy Taha, Khalid Ali Khan, Mohammad Javed Ansari, Soha A. Farag, Dalia M. B. Shawer, El-Said Mohamed Elnabawy. Effect of harvest season on the nutritional value of bee pollen protein. PLoS ONE. 2020; 15 (12):e0241393.

Chicago/Turabian Style

Saad N. Al-Kahtani; El-Kazafy Taha; Khalid Ali Khan; Mohammad Javed Ansari; Soha A. Farag; Dalia M. B. Shawer; El-Said Mohamed Elnabawy. 2020. "Effect of harvest season on the nutritional value of bee pollen protein." PLoS ONE 15, no. 12: e0241393.

Journal article
Published: 19 November 2020 in Journal of King Saud University - Science
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The Chichali Formation encountered in Chanda 01 well, located in the Shakardara Kohat, Khyber Pakhtunkhwa Province of Pakistan has been studied in detail as a source for hydrocarbon. Nine drill cuttings of the formation were subjected to geochemical analyses and organic petrography. Various screening techniques including TOC analysis by using Leco-CS analyzer, Rock Eval pyrolysis and organic petrography were used to determine the quality, quantity, maturity and depositional environment of the organic matter. The total organic carbon (TOC) of samples from Chichali Formation ranging from 0.47 to 2.76 wt% indicate fair to very good source rock. Pyrolysis parameters resulted in S1values ranging from 0.21 to 0.83 mg/g of rock, S2 0.44 to 0.61 mg/g rock, PI 0.32–0.51 and Tmax 442–448 °C. The Tmax and vitrinite reflectance data (0.72–0.84 Ro %) indicate that Chichali Formation is effective source rock and is in the process of generating hydrocarbons and falls within oil window. Geochemical parameters Hydrogen index (HI), Oxygen index (OI), Tmax along with organic petrography analyses (maceral) are used for kerogen type evaluation. The calculated HI ranges between 31 and 54 mg HC/g TOC and OI 30–96 mg O2/g TOC. Using modified Van Krevlen diagram between HI vs OI of Rock Eval pyrolysis and maceral analyses, kerogen type II/III and transition between anoxic to oxic depositional environments is interpreted for the studied Chichali Formation.

ACS Style

Syed Mamoon Siyar; Muhammad Zafar; Samina Jahandad; Tahseenullah Khan; Fayaz Ali; Sajjad Ahmad; Mohammed S. Fnais; Kamal Abdelrahman; Mohammad Javed Ansari. Hydrocarbon generation potential of Chichali Formation, Kohat Basin, Pakistan: A case study. Journal of King Saud University - Science 2020, 33, 101235 .

AMA Style

Syed Mamoon Siyar, Muhammad Zafar, Samina Jahandad, Tahseenullah Khan, Fayaz Ali, Sajjad Ahmad, Mohammed S. Fnais, Kamal Abdelrahman, Mohammad Javed Ansari. Hydrocarbon generation potential of Chichali Formation, Kohat Basin, Pakistan: A case study. Journal of King Saud University - Science. 2020; 33 (1):101235.

Chicago/Turabian Style

Syed Mamoon Siyar; Muhammad Zafar; Samina Jahandad; Tahseenullah Khan; Fayaz Ali; Sajjad Ahmad; Mohammed S. Fnais; Kamal Abdelrahman; Mohammad Javed Ansari. 2020. "Hydrocarbon generation potential of Chichali Formation, Kohat Basin, Pakistan: A case study." Journal of King Saud University - Science 33, no. 1: 101235.

Research article
Published: 21 July 2020 in PLOS ONE
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Cotton (Gossypium hirsutum L.), being a cash and fiber crop is of high significance in Pakistan. Numerous insect pests and viral diseases in Pakistan and around the world attack cotton crop. Genetically modified cotton (transgenic, resistant to lepidopteran insects), hereafter written as ‘Bt-cotton’ has been introduced in many regions of the world to combat bollworms. However, cultivars differ in their pest susceptibility, yield response and fiber quality traits. Nonetheless, recent studies have indicated that lepidopteran pests are evolving resistance against ‘Bt-cotton’. Several ‘Bt-cotton’ cultivars have been developed in Pakistan in the past decade; however, limited is known about their pest susceptibility, seed-cotton yield and fiber quality traits. This two-year field study evaluated pest susceptibility, yield and fiber quality traits of thirteen newly developed ‘Bt-cotton’ cultivars in Pakistan. The cultivars differed in their susceptibility to sucking insects during both years of study. The cultivars ‘FH-647’, ‘SLH-8’, ‘FH-Lalazar’ and ‘IUB-013’ were more susceptible to jassid, whereas ‘BS-52’ exhibited higher susceptibility to whitefly during both years of study. Similarly, cultivars ‘AGC-999’ and ‘MNH-992’ proved highly susceptible to thrips during each study year. Although ‘Bt-cotton’ is resistant to bollworms, cultivars ‘SLAH-8’, ‘VH-305’ and ‘BH-184’ were susceptible to spotted bollworm, while ‘SLAH-8’, ‘RH-647’ and ‘VH-305’ were infested by American bollworm. The most susceptible cultivars to cotton leaf curl virus (CLCuV) attack were ‘RH-647’, ‘IR-NIBGE-7’ and ‘VH-305’. The highest seed-cotton yield was recorded for ‘FH-Lalazar’ during both years of study. Similarly, the highest ginning out turn was recorded for cultivars ‘BS-52’, ‘VH-305’, ‘RH-647’, ‘IUB’ and ‘AA-919’. The cultivar ‘FH-Lalazar’ exhibited low pest susceptibility and CLCuV infestation compared to the rest of cultivars. The highest and the lowest gross and net incomes and benefit:cost ratio were noted for ‘FH-Lalazar’ and ‘RH-647, respectively. Keeping in view the low pest susceptibility and high seed-cotton yield, ‘FH-Lalazar’ could be recommended for higher yield and economic returns in Multan, Pakistan. Nonetheless, regional trials should be conducted for site-specific or region-specific recommendations.

ACS Style

Haider Karar; Muhammad Amjad Bashir; Muneeba Haider; Najeeba Haider; Khalid Ali Khan; Hamed A. Ghramh; Mohammad Javed Ansari; Çetin Mutlu; Suilman Mohammad Alghanem. Pest susceptibility, yield and fiber traits of transgenic cotton cultivars in Multan, Pakistan. PLOS ONE 2020, 15, e0236340 .

AMA Style

Haider Karar, Muhammad Amjad Bashir, Muneeba Haider, Najeeba Haider, Khalid Ali Khan, Hamed A. Ghramh, Mohammad Javed Ansari, Çetin Mutlu, Suilman Mohammad Alghanem. Pest susceptibility, yield and fiber traits of transgenic cotton cultivars in Multan, Pakistan. PLOS ONE. 2020; 15 (7):e0236340.

Chicago/Turabian Style

Haider Karar; Muhammad Amjad Bashir; Muneeba Haider; Najeeba Haider; Khalid Ali Khan; Hamed A. Ghramh; Mohammad Javed Ansari; Çetin Mutlu; Suilman Mohammad Alghanem. 2020. "Pest susceptibility, yield and fiber traits of transgenic cotton cultivars in Multan, Pakistan." PLOS ONE 15, no. 7: e0236340.