Incorporation of various alternative resources as co-digestion substrates aids to reduce the consumption of agricultural crops for biogas production. However, the efficiency and limitations of these co-substrates is still not fully understood. Use of biomass waste remaining after enzymatic hydrolysis for high value chemical fermentation, meat processing and dairy wastewater primary sludge as co-substrates in an agricultural resource anaerobic digestion plant is tackled within this study. The results showed that anionic surfactants (<200 ppm) can be used to improve fat, oil and grease (FOG) solubility in water and, at the same time, enhance the biomethane potential of FOG-containing sludge by increasing it from 1374.5 to 1765 mLCH₄/gVS for meat processing wastewater primary sludge, and from 534 to 740 mLCH₄/gVS for dairy wastewater primary sludge, when agricultural digestate is used as a substrate and sludge loading is not more than 10% from the volatile solids loaded. At the same time, only 549.7 mLCH₄/gVS was produced as 30-day BMP when 5% biomass hydrolysis waste was used. Biomass hydrolysis waste co-digestion with primary sludge from dairy and meat processing wastewaters has an antigenic effect, and separate substrate anaerobic digestion gave a better results, thus, showing that excessive combination of various waste resources can be inhibitory for biogas production and the appropriate substrate selection and combination is a technical challenge for the biogas industry.

Semi-natural grassland habitats are most often limited to animal grazing and low intensity farming. Their potential in bioenergy production is complicated due to the heterogeneity, variation, accessibility, and need for complex pre-treatment/hydrolysis techniques to convert into valuable products. In this research, fermentable sugar production efficiency from various habitats at various vegetation periods was evaluated. The highest fermentable sugar yields (above 0.2 g/g volatile solids) over a period of 3 years were observed from habitats “xeric and calcareous grasslands” (Natura 2000 code: 6120) and “semi-natural dry grasslands and scrubland facies on calcareous substrates” (Natura 2000 code: 6210). Both had a higher proportion of dicotyledonous plants. At the same time, the highest productivity (above 0.7 t sugar/ha) was observed from lowland hay meadows in the initial stage of the vegetation. Thus, despite variable yield-affecting factors, grasslands can be a potential resource for energy production.

Use of Alkali-activated Aluminosilicatematerialto Enhancebiogas Production from Acidic Whey