Prostate-specific membrane antigen (PSMA) is a glycoprotein present in the prostate, that is overexpressed in prostate cancer (PCa). Recently, PSMA-directed radiopharmaceuticals have been developed, allowing the pinpointing of tumors with the Positron Emission Tomography (PET) or Single Photon Emission Computed Tomography (SPECT) imaging techniques. The aim of the present work was to standardize and validate an automatic synthesis module-based radiolabeling protocol for [⁶⁸Ga]Ga-PSMA-11, as well as to produce a radiopharmaceutical for PET imaging of PCa malignancies. [⁶⁸Ga]Ga-PSMA-11 was evaluated to determine the radiochemical purity (RCP), stability in saline solution and serum, lipophilicity, affinity to serum proteins, binding and internalization to lymph node carcinoma of the prostate (LNCaP) cells, and ex vivo biodistribution in mice. The radiopharmaceutical was produced with an RCP of 99.06 ± 0.10%, which was assessed with reversed-phase high-performance liquid chromatography (RP-HPLC). The product was stable in saline solution for up to 4 h (RCP > 98%) and in serum for up to 1 h (RCP > 95%). The lipophilicity was determined as −3.80 ± 0.15, while the serum protein binding (SPB) was 8.5 and a tumor-to-blood ratio of >3.5. In conclusion, an automatic synthesis module-based radiolabeling protocol for [⁶⁸Ga]Ga-PSMA-11 was standardized and the product was evaluated, thus verifying its characteristics for PET imaging of PCa tumors in a clinical environment.

Background: Chronic Chagas cardiomyopathy in humans is characterized by segmental left ventricular wall motion abnormalities ( WMA ), mainly in the early stages of disease. This study aimed at investigating the detection of WMA and its correlation with the underlying histopathological changes in a chronic Chagas cardiomyopathy model in hamsters. Methods and Results: Female Syrian hamsters (n=34) infected with 3.5×10 ⁴ or 10 ⁵ blood trypomastigote Trypanosoma cruzi (Y strain) forms and an uninfected control group (n=7) were investigated. After 6 or 10 months after the infection, the animals were submitted to in vivo evaluation of global and segmental left ventricular systolic function by echocardiography, followed by euthanasia and histological analysis for quantitative assessment of fibrosis and inflammation with tissue sampling in locations coinciding with the left ventricular wall segmentation employed at the in vivo echocardiographic evaluation. Ten of the 34 infected animals (29%) showed reduced left ventricular ejection fraction (<73%). Left ventricular ejection fraction was more negatively correlated with the intensity of inflammation ( r =−0.63; P <0.0001) than with the extent of fibrosis ( r =−0.36; P =0.036). Among the 24 animals with preserved left ventricular ejection fraction (82.9±5.5%), 8 (33%) showed segmental WMA predominating in the apical, inferior, and posterolateral segments. The segments exhibiting WMA , in comparison to those with normal wall motion, showed a greater extent of fibrosis (9.3±5.7% and 7±6.3%, P <0.0001) and an even greater intensity of inflammation (218.0±111.6 and 124.5±84.8 nuclei/mm², P <0.0001). Conclusions: Isolated WMA with preserved global systolic left ventricular function is frequently found in Syrian hamsters with experimental chronic Chagas cardiomyopathy whose underlying histopathological features are mainly inflammatory.

Altered myocardial perfusion is a common finding in chronic Chagas cardiomyopathy (CCC), but its underlying histologic changes have not been elucidated. We investigated the occurrence of myocardial perfusion defects (MPDs) and the correlated regional changes to histology in an experimental model of CCC in hamsters. Methods: Female Syrian hamsters (n = 34) were infected with 3.5 × 10⁴ to 10⁵ trypomastigote forms of Trypanosoma cruzi, Y strain, and 6–10 mo afterward underwent in vivo imaging including resting ^99mTc-sestamibi SPECT, segmental and global left ventricular function assessment using 2-dimensional echocardiography, and ¹⁸F-FDG PET for evaluation of myocardial viability. Histologic analysis included quantification of fibrosis, inflammatory infiltration, and the diameter and density of myocardial microcirculation. Results: MPDs were present in 17 (50%) of the infected animals. Histologic analysis revealed no transmural scar in segments with an MPD, and normal or mildly reduced ¹⁸F-FDG uptake, indicating viable myocardium. Infected animals with an MPD, in comparison to infected animals without an MPD and control animals, showed a lower left ventricular ejection fraction (P = 0.012), a higher wall motion score index (P = 0.004), and a higher extent of inflammatory infiltration (P = 0.018) but a similar extent of fibrosis (P = 0.15) and similar microvascular diameter and density (P > 0.05). Segments with an MPD (n = 65), as compared with normally perfused regions in the same animal (n = 156), showed a higher wall motion score index (P = 0.005) but a similar extent of inflammatory infiltration, a similar extent of fibrosis, and a similar microvascular diameter and density. Conclusion: Resting MPDs are frequent in experimental CCC and are associated with myocardial inflammation but do not designate scar tissue, corresponding to regions with metabolically viable myocardium.

The Background Emission Anisotropy Scanning Telescope (BEAST) is a millimeter wavelength experiment designed to generate maps of fluctuations in the cosmic microwave background (CMB). The telescope is composed of an off-axis Gregorian optical system with a 2.2 m primary that focuses the collected microwave radiation onto an array of cryogenically cooled high electron mobility transistor (HEMT) receivers. This array is composed of six corrugated scalar feed horns in the Q band (38 to 45 GHz) and two more in the Ka band (26 to 36 GHz) with one of the six Q-band horns connected to an ortho-mode transducer for extraction of both polarizations incident on the single feed. The system has a minimum beam size of 200 with an average sensitivity of 900 mu K root s per receiver. This paper describes the design and performance of the BEAST instrument and provides the details of subsystems developed and used toward the goal of generating a map of CMB fluctuations on 200 scales with sensitivity in l space between l similar to 100 and l similar to 500. A map of the CMB centered on the north celestial pole has been generated from the BEAST telescope in a 9 degrees wide annulus at declination 37 degrees with a typical pixel error of 57 +/- 5 mu K when smoothed to 300 resolution. A brief summary of the map and results generated by an observing campaign at the University of California White Mountain Research Station are also included

We present the first sky maps from the BEAST (Background Emission Anisotropy Scanning Telescope) experiment. BEAST consists of a 2.2 meter off axis Gregorian telescope fed by a cryogenic millimeter wavelength focal plane currently consisting of 6 Q band (40 GHz) and 2 Ka band (30 GHz) scalar feed horns feeding cryogenic HEMT amplifiers. Data were collected from two balloon-borne flights in 2000, followed by a lengthy ground observing campaign from the 3.8 Km altitude University of California White Mountain Research Station. This paper reports the initial results from the ground based observations. The instrument produced an annular map covering the sky from declinateion 33 to 42 degrees. The maps cover an area of 2470 square degrees with an effective resolution of 23 arcminutes FWHM at 40 GHz and 30 arcminutes at 30 GHz. The map RMS (smoothed to 30 arcminutes and excluding galactic foregrounds) is 54 +-5 microK at 40 GHz. Comparison with the instrument noise gives a cosmic signal RMS contribution of 28 +-3 microK. An estimate of the actual CMB sky signal requires taking into account the l-space filter function of our experiment and analysis techniques, carried out in a companion paper (O'Dwyer et al. 2003). In addition to the robust detection of CMB anisotropies, we find a strong correlation between small portions of our maps and features in recent H$\alpha$ maps (Finkbeiner, 2003). In this work we describe the data set and analysis techniques leading to the maps, including data selection, filtering, pointing reconstruction, mapmaking algorithms and systematic effects. A detailed description of the experiment appears in Childers et al. (2003).

The main objective of the present study was to upgrade a clinical gamma camera to obtain high resolution tomographic images of small animal organs. The system is based on a clinical gamma camera to which we have adapted a special-purpose pinhole collimator and a device for positioning and rotating the target based on a computer-controlled step motor. We developed a software tool to reconstruct the target’s three-dimensional distribution of emission from a set of planar projections, based on the maximum likelihood algorithm. We present details on the hardware and software implementation. We imaged phantoms and heart and kidneys of rats. When using pinhole collimators, the spatial resolution and sensitivity of the imaging system depend on parameters such as the detector-to-collimator and detector-to-target distances and pinhole diameter. In this study, we reached an object voxel size of 0.6 mm and spatial resolution better than 2.4 and 1.7 mm full width at half maximum when 1.5- and 1.0-mm diameter pinholes were used, respectively. Appropriate sensitivity to study the target of interest was attained in both cases. Additionally, we show that as few as 12 projections are sufficient to attain good quality reconstructions, a result that implies a significant reduction of acquisition time and opens the possibility for radiotracer dynamic studies. In conclusion, a high resolution single photon emission computed tomography (SPECT) system was developed using a commercial clinical gamma camera, allowing the acquisition of detailed volumetric images of small animal organs. This type of system has important implications for research areas such as Cardiology, Neurology or Oncology.