Enhancing fruit sugar contents, especially for high-flavonoid apples with a sour taste, is one of the main goals of horticultural crop breeders. This study analyzed sugar accumulation and the underlying mechanisms in the F₂ progenies of a hybridization between the high-sugar apple (Malus × domestica) variety ‘Gala’ and high-flavonoid apple germplasm ‘CSR6R6’. We revealed that MdSWEET9b (sugars will eventually be exported transporter) helps mediate sugar accumulation in fruits. Functional characterization of MdSWEET9b in yeast mutants lacking sugar transport as well as in overexpressing and CRISPR/Cas9 knockdown apple calli revealed MdSWEET9b could transport sucrose specifically, ultimately promoting normal yeast growth and accumulation of total sugar contents. Moreover, MdWRKY9 bound to the MdSWEET9b promoter and regulated its activity, which responded to abscisic acid (ABA) signaling. Furthermore, MdWRKY9 interacted with MdbZIP23 (basic leucine zipper) and MdbZIP46, key ABA signal transducers, at the protein and DNA levels to enhance its regulatory effect on MdSWEET9b expression, thereby influencing sugar accumulation. Based on the contents of ABA in lines with differing sugar contents and the effects of ABA treatments on fruits and calli, we revealed ABA as one of the main factors responsible for the diversity in apple fruit sugar content. The results of this study have clarified how MdSWEET9b influences fruit sugar accumulation, while also further elucidating the regulatory effects of the ABA signaling network on fruit sugar accumulation. This work provides a basis for future explorations of the cross-talk between hormone and sugar metabolism pathways.

Salt stress is a major adverse abiotic factor seriously affecting fruit tree growth and development. It ultimately lowers fruit quality and reduces yield. Phosphatidylcholine (PC) is an important cell membrane component that is critical for cell structure and membrane stability maintenance. In this study, we found that the addition of external PC sources significantly increased the tolerance of one-year-old peach trees, Prunus persica (L.) Batsch., to salt stress and attenuated their damage. The effect of exogenous application of 200 mg/L PC exerted the most significant positive effect. Its use caused seedling leaf stomatal opening, contributing to normal gas exchange. Moreover, beneficial effects were exerted also to the root system, which grew normally under salt stress. Meanwhile, phospholipase D activity in the cell was promoted. The production of phosphatidic acid (PA) was enhanced by increased decomposition of phospholipids; PA serves as a secondary messenger involved in plant biological process regulation and the reduction in the reactive oxygen species- and peroxide-induced damage caused by salt stress. The possible mechanism of action is via promoted plant osmotic regulation and tolerance to salt stress, reducing salt stress-induced injury to plants.

In strawberry, sucrose is the major form of carbohydrate translocated from the leaves to the fruits and plays an important role in fruit ripening. As a conserved energy sensor, sucrose nonfermenting-1 (SNF1)-related kinase 1 (SnRK1) plays an important role in plant carbon metabolism. However, evidence that SnRK1 regulates sucrose accumulation in fruits is lacking. In this study, we transiently expressed FaSnRK1α in strawberry fruits and found that overexpression (OE) of the FaSnRK1α gene significantly increased the sucrose content, whereas repression of FaSnRK1α by RNA interference (RNAi) decreased the sucrose content. Further analysis revealed that FaSnRK1α increased the expression of FaSUS1 and FaSUS3 as well as the activity of sucrose synthase (SUS; EC 2.4.1.13) and that FaSPS1 expression and sucrose phosphate synthase (SPS; EC 2.4.1.14) activity were strongly downregulated, which decreased the accumulation of sucrose. However, the expression of FaSPS3, which is reported to contribute to sucrose accumulation, was induced by FaSnRK1α, and FaNI expression and invertase (INV; EC 3.2.1.26) activity were upregulated by FaSnRK1α. In addition, FaSnRK1α positively upregulated the expression of the sucrose transporter (SUT) genes FaSUT1 and FaSUT5 and interacted with FaSUS1, FaSPS1 and FaSPS3 proteins but not with FaSUS3, FaNI, FaSUT1 or FaSUT5 proteins. Overall, FaSnRK1α systematically regulates the expression of the genes and activities of key enzymes involved in the sucrose metabolic pathway and promotes the long-distance transport of sucrose, thereby increasing sucrose accumulation and ultimately promoting fruit ripening. However, the mechanisms by which sucrose transport and degradation are regulated by SnRK1 warrant additional research.

Fruit quality depends largely on the type and amount of sugar accumulated in the fruit. In peach [Prunus persica (L.) Batsch], sorbitol is the main photosynthetic product and plays a crucial role in sugar metabolism. As a conserved energy sensor, SNF1-related kinase 1 (SnRK1) is involved in the regulation of carbon metabolism. In this study, SnRK1 was able to respond to induction by treatment with exogenous trehalose and sorbitol on ‘Ruipan 17’ peach fruit. After treatment with 100-mM trehalose for 3 h, the SnRK1 activity decreased by 18% and the activities of sorbitol dehydrogenase (SDH) and sucrose synthase (SS) also decreased significantly, but sucrose phosphate synthase (SPS) activity increased significantly; whereas sorbitol treatment under the same conditions resulted in a 12.6% increase in SnRK1 activity and the activities of SDH and SS synthase also increased significantly, compared with the control. The contents of glucose, fructose and sucrose in peach fruit increased significantly after 3 h of sorbitol treatment. In addition, the interactions between PpSnRK1α and enzymes PpSDH and PpSPS were confirmed by yeast two-hybrid method and the phosphorylation of PpSnRK1α and PpSDH was detected in vitro. Taken together, these results suggest that SnRK1 promotes sorbitol metabolism by activating SDH and it also regulates the activities of SS and SPS that enhance sucrose accumulation in peach fruit. SnRK1 protein kinase is involved in sugar metabolism and has the potential to be used for improving fruit quality.