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Raquel López-Arellano
Laboratorio No 5: LEDEFAR, Unidad de Investigación Multidisciplinaria, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Cuautitlán Izcalli 54714, Mexico

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Journal article
Published: 23 July 2021 in Vaccines
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Avian reovirus (ARV) is the principal cause of several diseases. The vaccination of breeders allows for the control of viral arthritis and delivery of maternal-derived antibodies to the progeny. The vaccination of broiler chickens with ARV strain S1133 is used to prevent viral arthritis. However, the post-vaccination enteric effects have not been well-characterized. The purpose of this study was to evaluate the effect of vaccination with the S1133 strain on the weight gain and feed conversion of broiler chickens and to characterize the gastric, enteric, and pancreatic lesions that the strain could induce. A total of 672,000 chickens were divided into two groups: a group vaccinated with ARV strain S1133 (S1133ARV) and a control group (not vaccinated). Upon histological analysis, the vaccine group showed less proventricular glandular tissue and atrophy of the pancreas and duodenal villi, as well as having a lower average daily profit. The conclusion based on the results of this investigation is that neonatal vaccination with S1133ARV causes atrophy of the pancreatic acini, proventricular glands, and intestinal villi, leading to an increased diameter of the glandular lumen and atrophy of the enteric villous, as well as weight loss, in broiler chickens.

ACS Style

Victor Petrone-Garcia; Joshua Gonzalez-Soto; Raquel Lopez-Arellano; Mariano Delgadillo-Gonzalez; Victor Valdes-Narvaez; Fernando Alba-Hurtado; Xochitl Hernandez-Velasco; Inkar Castellanos-Huerta; Guillermo Tellez-Isaias. Evaluation of Avian Reovirus S1133 Vaccine Strain in Neonatal Broiler Chickens in Gastrointestinal Integrity and Performance in a Large-Scale Commercial Field Trial. Vaccines 2021, 9, 817 .

AMA Style

Victor Petrone-Garcia, Joshua Gonzalez-Soto, Raquel Lopez-Arellano, Mariano Delgadillo-Gonzalez, Victor Valdes-Narvaez, Fernando Alba-Hurtado, Xochitl Hernandez-Velasco, Inkar Castellanos-Huerta, Guillermo Tellez-Isaias. Evaluation of Avian Reovirus S1133 Vaccine Strain in Neonatal Broiler Chickens in Gastrointestinal Integrity and Performance in a Large-Scale Commercial Field Trial. Vaccines. 2021; 9 (8):817.

Chicago/Turabian Style

Victor Petrone-Garcia; Joshua Gonzalez-Soto; Raquel Lopez-Arellano; Mariano Delgadillo-Gonzalez; Victor Valdes-Narvaez; Fernando Alba-Hurtado; Xochitl Hernandez-Velasco; Inkar Castellanos-Huerta; Guillermo Tellez-Isaias. 2021. "Evaluation of Avian Reovirus S1133 Vaccine Strain in Neonatal Broiler Chickens in Gastrointestinal Integrity and Performance in a Large-Scale Commercial Field Trial." Vaccines 9, no. 8: 817.

Original paper
Published: 15 March 2021 in Acta Parasitologica
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(1) To assess the in vitro activity of Artemisia cina against Haemonchus contortus L3 (HcL3) and in transitional (L3–L4) larvae (HcTrL3–L4); (2) to quantify the relative expression of the Hc29 gene in HcTrL3–L4 exposed to the A. cina n-hexane extract; and (3) to assess the anthelmintic activity (AA) of the A. cina organic extracts in gerbils artificially infected with H. contortus (HcArt/inf/gerbs). The in vitro assay was carried out in 96-well microtitration plates. The following A. cina extracts: ethyl acetate (Ac-EtOAcEx), n-hexane (Ac-n-HexEx), and methanol (Ac-MethEx) were assessed at 1 and 2 mg/mL against HcL3 and HcTrL3–L4 at 24 h exposure. Relative expression of the Hc29 gene in HcTrL3–L4 was obtained by RT-PCR. For assessing the AA, six groups of five HcArt/inf/gerbs were used. Groups were treated orally with 4 mg/kg BW of A. cina extracts. Five days after treatment, the gerbils were necropsied and nematodes counted. The highest in vitro activities (75 and 82.6%) were shown by Ac-n-HexEx at 1 and 2 mg/mL, respectively. For HcTrL3-L4 the highest in vitro activities (69 and 23%) were shown by Ac-n-HexEx and isoguaiacine at 0.625 mg/mL, respectively. Also, upregulation of H. contortus Hc29 gene by 13- and 80-fold (p < 0.01) was observed on the HcTrL3–L4 stage after exposure to Ac-n-HexEx extract and isoguaiacine at 0.078 mg/mL, respectively. Reduction percentage was 100% in HcArt/inf/gerbs treated with Ac-n-HexEx. We conclude that the Ac-n-HexEx and isoguaiacine compound had anthelmintic efficacy against H. contortus and L3 and HcTrL3–L4.

ACS Style

Rosa Isabel Higuera-Piedrahita; Mariana Dolores-Hernández; Luis Gerardo Jiménez-Pérez; Brígida C. Camacho-Enríquez; Alejandro Zamilpa; Raquel López-Arellano; Pedro Mendoza-De-Gives; Jorge A. Cuéllar-Ordaz; Ma. Eugenia López-Arellano. In Vitro Nematocidal Effect and Anthelmintic Activity of Artemisia cina Against Haemonchus contortus in Gerbils and Relative Expression of Hc29 Gene in Transitional Larvae (L3–L4). Acta Parasitologica 2021, 66, 938 -946.

AMA Style

Rosa Isabel Higuera-Piedrahita, Mariana Dolores-Hernández, Luis Gerardo Jiménez-Pérez, Brígida C. Camacho-Enríquez, Alejandro Zamilpa, Raquel López-Arellano, Pedro Mendoza-De-Gives, Jorge A. Cuéllar-Ordaz, Ma. Eugenia López-Arellano. In Vitro Nematocidal Effect and Anthelmintic Activity of Artemisia cina Against Haemonchus contortus in Gerbils and Relative Expression of Hc29 Gene in Transitional Larvae (L3–L4). Acta Parasitologica. 2021; 66 (3):938-946.

Chicago/Turabian Style

Rosa Isabel Higuera-Piedrahita; Mariana Dolores-Hernández; Luis Gerardo Jiménez-Pérez; Brígida C. Camacho-Enríquez; Alejandro Zamilpa; Raquel López-Arellano; Pedro Mendoza-De-Gives; Jorge A. Cuéllar-Ordaz; Ma. Eugenia López-Arellano. 2021. "In Vitro Nematocidal Effect and Anthelmintic Activity of Artemisia cina Against Haemonchus contortus in Gerbils and Relative Expression of Hc29 Gene in Transitional Larvae (L3–L4)." Acta Parasitologica 66, no. 3: 938-946.

Journal article
Published: 07 November 2020 in Pathogens
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Mycoplasma synoviae (MS) and Mycoplasma gallisepticum (MG) strains were isolated at 39.5 °C to rule out temperature-sensitive strains (ts+) and identified using random amplification of polymorphic DNA. Then, their minimum inhibitory concentrations (MIC100) were calculated in isolated strains from broiler breeders and laying hens vaccinated with ts+ MS-H and ts+ MG TS-11 vaccines in Mexico. We sampled 631 lots of hens. A total of 28 of the 123 MS isolates and 12 of the 23 MG isolates were analyzed using random amplification of polymorphic DNA, of which 24 and 3 matched the DNA banding patterns of the MS-H and MG-F strains, respectively. The isolated MS and MG strains were sensitive to tiamulin and tylosin and showed intermediate sensitivity or resistance to lincomycin, florfenicol, erythromycin, enrofloxacin, and curcumin. Although both the MS and MG strains were sensitive to the same antibiotics (MIC100 lower than 1 mg mL−1), the MG strains were 5 to 10 times more sensitive than the MS strains. MS is the most frequently isolated mycoplasma in Mexican poultry production. The MS vaccine used (ts+ MS-H) could reverse its thermosensitivity and therefore could regain its virulence. MS was less sensitive to tiamulin and tylosin compared to MG.

ACS Style

Víctor M. Petrone-Garcia; Guillermo Tellez-Isaias; Fernando Alba-Hurtado; Christine N. Vuong; Raquel Lopez-Arellano. Isolation and Antimicrobial Sensitivity of Mycoplasma synoviae and Mycoplasma gallisepticum from Vaccinated Hens in Mexico. Pathogens 2020, 9, 924 .

AMA Style

Víctor M. Petrone-Garcia, Guillermo Tellez-Isaias, Fernando Alba-Hurtado, Christine N. Vuong, Raquel Lopez-Arellano. Isolation and Antimicrobial Sensitivity of Mycoplasma synoviae and Mycoplasma gallisepticum from Vaccinated Hens in Mexico. Pathogens. 2020; 9 (11):924.

Chicago/Turabian Style

Víctor M. Petrone-Garcia; Guillermo Tellez-Isaias; Fernando Alba-Hurtado; Christine N. Vuong; Raquel Lopez-Arellano. 2020. "Isolation and Antimicrobial Sensitivity of Mycoplasma synoviae and Mycoplasma gallisepticum from Vaccinated Hens in Mexico." Pathogens 9, no. 11: 924.

Journal article
Published: 10 November 2019 in Pathogens
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Two experimental models were conducted to evaluate and compare the effect of ascorbic acid (AA) or curcumin formulated in a solid dispersion (SD-CUR) as prophylactic or therapeutic alternatives to prevent or control S. Enteritidis (SE) infection in broiler chickens. In the prophylactic model, dietary administration of AA showed a significant reduction in SE counts in crop compared to the positive control (PC) group (p < 0.05), whereas in cecal tonsils (CT), SD-CUR significantly reduced SE recovery. Superoxide dismutase (SOD) activity was significantly higher in chickens supplemented with AA or SD-CUR, and total intestinal IgA levels were significantly lower in both treatments when compared to the PC group. Serum fluorescein isothiocyanate-dextran (FITC-d) levels were reduced by SD-CUR compared to PC, while AA presented significantly lower total aerobic bacteria. In the therapeutic model, only the dietary administration of AA significantly decreased SE in crop and CT on days 3 and 10 post-challenge. FITC-d levels were significantly lower in both treated groups in comparison to PC, but IgA levels were significantly reduced only by AA. The results suggest that dietary AA and SD-CUR have different modes of action to reduce SE intestinal colonization in two different challenge models in broiler chickens.

ACS Style

Daniel Hernandez-Patlan; Bruno Solis-Cruz; Karine P. Pontin; Juan D. Latorre; Xochitl Hernandez-Velasco; Ruben Merino-Guzman; Abraham Mendez-Albores; Billy M. Hargis; Raquel Lopez-Arellano; Guillermo Tellez-Isaias. Evaluation of Ascorbic Acid or Curcumin Formulated in a Solid Dispersion on Salmonella Enteritidis Infection and Intestinal Integrity in Broiler Chickens. Pathogens 2019, 8, 229 .

AMA Style

Daniel Hernandez-Patlan, Bruno Solis-Cruz, Karine P. Pontin, Juan D. Latorre, Xochitl Hernandez-Velasco, Ruben Merino-Guzman, Abraham Mendez-Albores, Billy M. Hargis, Raquel Lopez-Arellano, Guillermo Tellez-Isaias. Evaluation of Ascorbic Acid or Curcumin Formulated in a Solid Dispersion on Salmonella Enteritidis Infection and Intestinal Integrity in Broiler Chickens. Pathogens. 2019; 8 (4):229.

Chicago/Turabian Style

Daniel Hernandez-Patlan; Bruno Solis-Cruz; Karine P. Pontin; Juan D. Latorre; Xochitl Hernandez-Velasco; Ruben Merino-Guzman; Abraham Mendez-Albores; Billy M. Hargis; Raquel Lopez-Arellano; Guillermo Tellez-Isaias. 2019. "Evaluation of Ascorbic Acid or Curcumin Formulated in a Solid Dispersion on Salmonella Enteritidis Infection and Intestinal Integrity in Broiler Chickens." Pathogens 8, no. 4: 229.

Research article
Published: 08 May 2019 in Drug Testing and Analysis
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Clenbuterol is known to improve competition resistance and muscular growth in athletes. Although it is an illegal drug, its use by farmers is widely spread to induce growth of their cattle. Thus, when clenbuterol is found in the urine of an athlete, there is doubt whether it was consumed with doping purposes or if it is due to the consumption of meat from a clenbuterol‐fed animal. Previous studies suggest that enantiomeric relationship of clenbuterol may be different according to the intake source. However, the enantiomeric relationship throughout a doping cycle or a continuous intake of contaminated meat has not yet been explored. In this first approximation, our aim was the development and validation of a sensitive and rapid method for the determination of S‐ (+) and R‐ (─) clenbuterol enantiomers to be used in a controlled study in rats fed for one week with contaminated meat or simulating a doping cycle. Enantiomers were measured using liquid chromatography coupled to mass spectrometry with a triple quadrupole analyzer (LC‐TQ‐MS) and were separated on an AGP Chiralpak column. The method was fully validated following the VICH (Veterinary International Conference on Harmonization guidelines) and was linear in the range of 12.5‐800 pg/mL with a correlation coefficient of ≥0.98 for each enantiomer, and with a limit of quantitation and detection (LOQ and LOD) of 12.5 pg/mL and 6.5 pg/mL, respectively, for both enantiomers. The application of this method pointed out the shift of the enantiomeric relationship in urine from rats during the first five days of the doping cycle compared to those fed with contaminated meat. This finding can be of substantial importance in further doping studies.

ACS Style

Hernández Mariana Dolores; Alma Villaseñor; Olmos Sofia Piña; Crisóforo Mercado Márquez; Benjamín Velasco Bejarano; María Eugenia Gonsebatt Bonaparte; Raquel López‐Arellano; Mariana Dolores Hernández; Sofia Piña Olmos. Evaluation of R‐ (−) and S‐ (+) Clenbuterol enantiomers during a doping cycle or continuous ingestion of contaminated meat using chiral liquid chromatography by LC‐TQ‐MS. Drug Testing and Analysis 2019, 11, 1238 -1247.

AMA Style

Hernández Mariana Dolores, Alma Villaseñor, Olmos Sofia Piña, Crisóforo Mercado Márquez, Benjamín Velasco Bejarano, María Eugenia Gonsebatt Bonaparte, Raquel López‐Arellano, Mariana Dolores Hernández, Sofia Piña Olmos. Evaluation of R‐ (−) and S‐ (+) Clenbuterol enantiomers during a doping cycle or continuous ingestion of contaminated meat using chiral liquid chromatography by LC‐TQ‐MS. Drug Testing and Analysis. 2019; 11 (8):1238-1247.

Chicago/Turabian Style

Hernández Mariana Dolores; Alma Villaseñor; Olmos Sofia Piña; Crisóforo Mercado Márquez; Benjamín Velasco Bejarano; María Eugenia Gonsebatt Bonaparte; Raquel López‐Arellano; Mariana Dolores Hernández; Sofia Piña Olmos. 2019. "Evaluation of R‐ (−) and S‐ (+) Clenbuterol enantiomers during a doping cycle or continuous ingestion of contaminated meat using chiral liquid chromatography by LC‐TQ‐MS." Drug Testing and Analysis 11, no. 8: 1238-1247.

Journal article
Published: 23 April 2019 in Journal of the Mexican Chemical Society
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Curcumin has gained great attention in the last decades due to its fascinating properties for humans, such as anti-inflammatory or as cytotoxic against cancer. These effects are also claimed for pets such as cats and dogs, where curcumin administration is a daily practice routine. However, curcumin presents poor oral bioavailability, driving scientists to look for new delivery systems. In the last decades, several analytical methods for the quantification of curcumin in plasma have been published. To our knowledge there are no published reports on the effect of the level of hemolysis in the determination of this compound. In the present paper, a highly specific, sensitive and selective method is presented using Molecular Reaction Monitoring (SRM) using positive ionization (ESI+) mode. Curcumin and clopidogrel bisulfate – used as internal standard (IS) – were separated on an Acquity UPLC BECH Shield RP 18 column (1.7µm, 2.1 X 100mm) with 0.1% formic acid in acetonitrile and water in proportion of 60:40 (v/v). The analyte transitions were 369.3→177.06 m/z for curcumin and 322→212.05 m/z for IS. The method was fully validated and showed good linearity (R2 ≥ 0.999) over the range of 3-160 ng/mL. The Relative Standard Deviation (RSD) were less than 6% for intra-and inter-day analysis and recovery spanned 85-95%. We proved that the degree of hemolysis impaired curcumin quantitation. This method was applied to test curcumin bioavailability in both a mucoadhesive nanocapsule formulation and traditional capsules in dogs that attended routine veterinary consultation.Resumen. La curcumina ha ganado gran atención en las últimas décadas debido a sus propiedades terapéuticas para los humanos, como antiinflamatorio o citotóxico contra el cáncer. Estos efectos también se observan en pequeñas especies como gatos y perros, donde la administración de curcumina se ha vuelto una alternativa. Sin embargo, la curcumina presenta una baja biodisponibilidad oral, lo que impulsa a los científicos a buscar nuevos sistemas de administración. En las últimas décadas, se han publicado varios métodos analíticos para la cuantificación de curcumina en plasma. Actualmente, no hay informes publicados sobre el efecto del grado de hemólisis en la determinación de este compuesto. En este trabajo se desarrolló un método específico, sensible y selectivo utilizando el Monitoreo de reacción seleccionado (SRM) en modo de ionización positiva (ESI +). La curcumina y el bisulfato de clopidogrel, utilizado como patrón interno (IS), se separaron en una columna Acquity UPLC BECH Shield RP 18 (1,7 μm, 2,1 X 100 mm) con ácido fórmico al 0,1% en acetonitrilo y agua a una proporción de 60:40 (v/v). Las transiciones de los analitos fueron 369.3 → 177.06 m/z para curcumina y 322 → 212.05 m/z para IS. El método fue validado y demostró ser lineal (r2 ≥ 0.999) en el rango de 3-160 ng/mL. La desviación relativa estándar (RSD) fue inferior al 6% para el análisis intra e interdía y el porcentaje de recuperación fue 85-95%. Se descubrió que el grado de hemólisis afecta la cuantificación de curcumina. El método desarrollado se aplicó para evaluar la biodisponibilidad de curcumina tanto en una formulación de nanocápsulas mucoadhesivas como en cápsulas tradicionales en perros que asistieron a consultas veterinarias de rutina.

ACS Style

Mariana Dolores; Alma Villaseñor; Alma Luisa Revilla Vázquez; Helgi Jung; Victor Hugo Santiago Rios; Raquel López-Arellano. Full validation of curcumin analytical method by LC-MS/MS points out that the degree of hemolysis in plasma affects the quantitation: application in dog plasma study. Journal of the Mexican Chemical Society 2019, 62, 1 .

AMA Style

Mariana Dolores, Alma Villaseñor, Alma Luisa Revilla Vázquez, Helgi Jung, Victor Hugo Santiago Rios, Raquel López-Arellano. Full validation of curcumin analytical method by LC-MS/MS points out that the degree of hemolysis in plasma affects the quantitation: application in dog plasma study. Journal of the Mexican Chemical Society. 2019; 62 (4):1.

Chicago/Turabian Style

Mariana Dolores; Alma Villaseñor; Alma Luisa Revilla Vázquez; Helgi Jung; Victor Hugo Santiago Rios; Raquel López-Arellano. 2019. "Full validation of curcumin analytical method by LC-MS/MS points out that the degree of hemolysis in plasma affects the quantitation: application in dog plasma study." Journal of the Mexican Chemical Society 62, no. 4: 1.

Journal article
Published: 22 April 2019 in Animals
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Two experiments were conducted to evaluate the effect of the prophylactic or therapeutic administration of a 0.1% mixture containing ascorbic acid and a solid dispersion of curcumin with polyvinylpyrrolidone and boric acid (AA-CUR/PVP-BA) against Salmonella Enteritidis (S. Enteritidis) in broiler chickens. A third experiment was conducted to evaluate the impact of the dietary administration of 0.1% AA-CUR/PVP-BA in a necrotic enteritis (NE) model in broiler chickens. The prophylactic administration of 0.1% AA-CUR/PVP-BA significantly decreased S. Enteritidis colonization in cecal tonsils (CT) when compared to the positive control group (PC, p < 0.05). The therapeutic administration of 0.1% AA-CUR/PVP-BA significantly reduced the concentration of S. Enteritidis by 2.05 and 2.71 log in crop and CT, respectively, when compared with the PC on day 10 post-S. Enteritidis challenge. Furthermore, the serum FITC-d concentration and total intestinal IgA levels were also significantly lower in chickens that received 0.1% AA-CUR/PVP-BA. Contrary, the PC group showed significantly higher total intestinal IgA levels compared to the negative control or AA-CUR/PVP-BA groups in the NE model. However, 0.1% AA-CUR/PVP-BA showed a better effect in reducing the concentration of S. Enteritidis when compared to the NE model. Further studies with higher concentration of AA-CUR/PVP-BA into the feed to extend these preliminary results are currently being evaluated.

ACS Style

Daniel Hernandez-Patlan; Bruno Solís-Cruz; Karine Patrin Pontin; Juan D. Latorre; Mikayla F. A. Baxter; Xochitl Hernandez-Velasco; Ruben Merino-Guzman; Abraham Méndez-Albores; Billy M. Hargis; Raquel Lopez-Arellano; Guillermo Tellez-Isaias. Evaluation of the Dietary Supplementation of a Formulation Containing Ascorbic Acid and a Solid Dispersion of Curcumin with Boric Acid against Salmonella Enteritidis and Necrotic Enteritis in Broiler Chickens. Animals 2019, 9, 184 .

AMA Style

Daniel Hernandez-Patlan, Bruno Solís-Cruz, Karine Patrin Pontin, Juan D. Latorre, Mikayla F. A. Baxter, Xochitl Hernandez-Velasco, Ruben Merino-Guzman, Abraham Méndez-Albores, Billy M. Hargis, Raquel Lopez-Arellano, Guillermo Tellez-Isaias. Evaluation of the Dietary Supplementation of a Formulation Containing Ascorbic Acid and a Solid Dispersion of Curcumin with Boric Acid against Salmonella Enteritidis and Necrotic Enteritis in Broiler Chickens. Animals. 2019; 9 (4):184.

Chicago/Turabian Style

Daniel Hernandez-Patlan; Bruno Solís-Cruz; Karine Patrin Pontin; Juan D. Latorre; Mikayla F. A. Baxter; Xochitl Hernandez-Velasco; Ruben Merino-Guzman; Abraham Méndez-Albores; Billy M. Hargis; Raquel Lopez-Arellano; Guillermo Tellez-Isaias. 2019. "Evaluation of the Dietary Supplementation of a Formulation Containing Ascorbic Acid and a Solid Dispersion of Curcumin with Boric Acid against Salmonella Enteritidis and Necrotic Enteritis in Broiler Chickens." Animals 9, no. 4: 184.

Journal article
Published: 16 February 2019 in Toxins
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To evaluate the effect of cellulosic polymers (CEL) and curcumin (CUR) on aflatoxin B1 (AFB1) toxic effects on performance, and the biochemical and immunological parameters in broiler chickens, 150 one-day-old male broiler chicks were randomly allocated into five groups with three replicates of 10 chickens per pen: Negative Control (feed); AFB1 (feed + 2 ppm AFB1); CUR (feed + 2 ppm AFB1 + Curcumin 0.2%); CEL (feed + 2 ppm AFB1 + 0.3% Cellulosic polymers); and, CEL + CUR (feed + 2 ppm AFB1 + 0.3% Cellulose polymers + 0.2% Curcumin). Every week, body weight, body weight gain, feed intake, and feed conversion ratio were calculated. On day 21, liver, spleen, bursa of Fabricius, and intestine from five broilers per replicate per group were removed to obtain relative organ weight. Histopathological changes in liver, several biochemical biomarkers, antibody titers, and muscle and skin pigmentation were also recorded. Dietary addition of 0.3% CEL and 0.2% CUR separately significantly diminished some of the toxic effects resulting from AFB1 on performance parameters, relative organs weight, histopathology, immune response, and serum biochemical variables (P < 0.05); however, the combination of CUR and CEL showed a better-integrated approach for the management of poultry health problems that are related with the consumption of AFB1, since they have different mechanisms of action with different positive effects on the responses of broiler chickens.

ACS Style

Bruno Solis-Cruz; Daniel Hernandez-Patlan; Victor M. Petrone; Karine P. Pontin; Juan D. Latorre; Eric Beyssac; Xochitl Hernandez-Velasco; Ruben Merino-Guzman; Casey Owens; Billy M. Hargis; Raquel Lopez-Arellano; Guillermo Tellez-Isaias. Evaluation of Cellulosic Polymers and Curcumin to Reduce Aflatoxin B1 Toxic Effects on Performance, Biochemical, and Immunological Parameters of Broiler Chickens. Toxins 2019, 11, 121 .

AMA Style

Bruno Solis-Cruz, Daniel Hernandez-Patlan, Victor M. Petrone, Karine P. Pontin, Juan D. Latorre, Eric Beyssac, Xochitl Hernandez-Velasco, Ruben Merino-Guzman, Casey Owens, Billy M. Hargis, Raquel Lopez-Arellano, Guillermo Tellez-Isaias. Evaluation of Cellulosic Polymers and Curcumin to Reduce Aflatoxin B1 Toxic Effects on Performance, Biochemical, and Immunological Parameters of Broiler Chickens. Toxins. 2019; 11 (2):121.

Chicago/Turabian Style

Bruno Solis-Cruz; Daniel Hernandez-Patlan; Victor M. Petrone; Karine P. Pontin; Juan D. Latorre; Eric Beyssac; Xochitl Hernandez-Velasco; Ruben Merino-Guzman; Casey Owens; Billy M. Hargis; Raquel Lopez-Arellano; Guillermo Tellez-Isaias. 2019. "Evaluation of Cellulosic Polymers and Curcumin to Reduce Aflatoxin B1 Toxic Effects on Performance, Biochemical, and Immunological Parameters of Broiler Chickens." Toxins 11, no. 2: 121.

Research article
Published: 31 January 2019 in Environmental and Molecular Mutagenesis
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Mexico City's Metropolitan Area (MCMA) includes Mexico City and 60 municipalities of the neighbor states. Inhabitants are exposed to emissions from over 5 million vehicles and stationary sources of air pollutants such as particulate matter (PM) and ozone. MCMA PM contains elemental (EC) and organic carbon (OC). OCs include polycyclic aromatic hydrocarbons (PAHs), many of which induce mutagenic and carcinogenic DNA adducts. Gestational exposure to air pollution has been associated with increased risk of intrauterine growth restriction, preterm birth or low birth weight risk and PAH‐DNA adducts. These effects also depend on the presence of risk alleles. We investigated the presence of bulky PAH‐DNA adducts, plasma 8‐iso‐PGF2α (8‐iso‐prostaglandin F2α) and risk allele variants in neonates cord blood and their non‐smoking mothers' leucocytes from families that were living in a highly polluted area during 2014‐2015. The presence of adducts was significantly associated with both PM2.5 and PM10 levels, mainly during the last trimester of gestation in both neonates and mothers, while the last month of pregnancy was significant for the association between ozone levels and maternal plasma 8‐iso‐PGF2α. Fetal CYP1B1*3 risk allele was associated with increased adduct levels in neonates while the presence of the maternal allele significantly reduced the levels of fetal adducts. Maternal NQO1*2 was associated with lower maternal levels of adducts. Our findings suggest the need to reduce actual PM limits in MCMA. We did not observe a clear association between PM and/or adduct levels and neonate weight, length, body mass index (BMI), Apgar or Capurro score. This article is protected by copyright. All rights reserved.

ACS Style

Jorge A. Maciel-Ruiz; Cristina López-Rivera; Rogelio Robles-Morales; Maria G. Veloz-Martínez; Raquel López-Arellano; Gabriela Rodríguez-Patiño; Pavel Petrosyan; Tzipe Govezensky; Ana M. Salazar; Patricia Ostrosky-Wegman; Regina Montero-Montoya; Maria E. Gonsebatt; R Montero. Prenatal exposure to particulate matter and ozone: Bulky DNA adducts, plasma isoprostanes, allele risk variants, and neonate susceptibility in the Mexico City Metropolitan Area. Environmental and Molecular Mutagenesis 2019, 60, 428 -442.

AMA Style

Jorge A. Maciel-Ruiz, Cristina López-Rivera, Rogelio Robles-Morales, Maria G. Veloz-Martínez, Raquel López-Arellano, Gabriela Rodríguez-Patiño, Pavel Petrosyan, Tzipe Govezensky, Ana M. Salazar, Patricia Ostrosky-Wegman, Regina Montero-Montoya, Maria E. Gonsebatt, R Montero. Prenatal exposure to particulate matter and ozone: Bulky DNA adducts, plasma isoprostanes, allele risk variants, and neonate susceptibility in the Mexico City Metropolitan Area. Environmental and Molecular Mutagenesis. 2019; 60 (5):428-442.

Chicago/Turabian Style

Jorge A. Maciel-Ruiz; Cristina López-Rivera; Rogelio Robles-Morales; Maria G. Veloz-Martínez; Raquel López-Arellano; Gabriela Rodríguez-Patiño; Pavel Petrosyan; Tzipe Govezensky; Ana M. Salazar; Patricia Ostrosky-Wegman; Regina Montero-Montoya; Maria E. Gonsebatt; R Montero. 2019. "Prenatal exposure to particulate matter and ozone: Bulky DNA adducts, plasma isoprostanes, allele risk variants, and neonate susceptibility in the Mexico City Metropolitan Area." Environmental and Molecular Mutagenesis 60, no. 5: 428-442.

Journal article
Published: 21 December 2018 in Journal of Pharmaceutical and Biomedical Analysis
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A new, rapid, simple and specific method to determine 5-chloro 8-hydroxyquinoline (5-HQ) and 5,7-dichloro 8-hydroxyquinoline (5,7-HQ) stability in swine feed was optimized and validated. A system consisting of an ACQUITY UPLC BEH C18 column (1.7 µm, 2.1 mm x 100 mm), a mobile phase of acetonitrile-0.1% o-phosphoric acid (55:45 v/v) with a 0.5 mL/min flow rate, and a PDA detector (247 nm) were used. The retention times of 5-HQ and 5,7-HQ, were 0.77 minutes and 1.6 minutes, respectively. The pure drug was subjected to acid and alkali hydrolysis, chemical oxidation and UV light degradation to perform forced degradation studies. 5,7-HQ was more susceptible to degradation than 5-HQ. The figures of merit of the method (linearity, accuracy, precision, and robustness) were determined. The method was successfully applied to estimate the stability of both analytes in medicated feed.

ACS Style

Miriam Aide Castillo Rodríguez; Gabriela Rodríguez Patiño; Alma Luisa Revilla Vázquez; Eduardo Rodríguez De San Miguel; Raquel López-Arellano; Castillo Rodríguez Miriam Aide; Rodríguez Patiño Gabriela; Revilla Vázquez Alma Luisa; Rodríguez De San Miguel Eduardo; López-Arellano Raquel. Validation of a UPLC-PDA method to study the content and stability of 5-chloro 8-hydroxyquinoline and 5,7-dichloro 8-hydroxyquinoline in medicated feed used in swine farming. Journal of Pharmaceutical and Biomedical Analysis 2018, 166, 113 -118.

AMA Style

Miriam Aide Castillo Rodríguez, Gabriela Rodríguez Patiño, Alma Luisa Revilla Vázquez, Eduardo Rodríguez De San Miguel, Raquel López-Arellano, Castillo Rodríguez Miriam Aide, Rodríguez Patiño Gabriela, Revilla Vázquez Alma Luisa, Rodríguez De San Miguel Eduardo, López-Arellano Raquel. Validation of a UPLC-PDA method to study the content and stability of 5-chloro 8-hydroxyquinoline and 5,7-dichloro 8-hydroxyquinoline in medicated feed used in swine farming. Journal of Pharmaceutical and Biomedical Analysis. 2018; 166 ():113-118.

Chicago/Turabian Style

Miriam Aide Castillo Rodríguez; Gabriela Rodríguez Patiño; Alma Luisa Revilla Vázquez; Eduardo Rodríguez De San Miguel; Raquel López-Arellano; Castillo Rodríguez Miriam Aide; Rodríguez Patiño Gabriela; Revilla Vázquez Alma Luisa; Rodríguez De San Miguel Eduardo; López-Arellano Raquel. 2018. "Validation of a UPLC-PDA method to study the content and stability of 5-chloro 8-hydroxyquinoline and 5,7-dichloro 8-hydroxyquinoline in medicated feed used in swine farming." Journal of Pharmaceutical and Biomedical Analysis 166, no. : 113-118.

Journal article
Published: 14 December 2018 in Research in Veterinary Science
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The objectives of the present study were to evaluate the antimicrobial effect of boric acid (BA) on Salmonella enteritidis colonization, intestinal permeability, total intestinal IgA levels, and cecal microbiota composition in broiler chickens. For this purpose, sixty day-old-chicks were randomly allocated to one of two groups: 1) non-treated control group and 2) 0.1% (w/w) BA in feed. All chicks were challenged with 104 cfu of S. Enteritidis per bird at 1-d old. On day 3 and 10 post-challenge, samples of crop and ceca-cecal tonsils (CCT) were aseptically collected to evaluate overall aerobic and lactic acid bacteria, as well as S. Enteritidis recovery. Blood and intestinal samples were also collected to evaluate intestinal permeability and total intestinal IgA production, respectively. On day 10 post-challenge, the concentration of S. Enteritidis in crop and CCT significantly decreased (P < .05) by 2.60 log10 and 5.26 log10, respectively, in the BA group compared to the control group. Furthermore, chickens treated with 0.1% BA had a significant reduction in serum fluorescein isothiocyanate dextran (FITC-d) concentration and lower total intestinal IgA levels when compared to the control group. Interestingly, in the microbiota analysis, only the abundance of Actinobacteria phylum was significantly lower in the BA group in comparison with the control group and significant differences in beta diversity were also observed between comparing groups. The results of the present study suggest that BA could maintain intestinal homeostasis, as well as the balance in the microbiota due to its effectiveness in controlling S. Enteritidis infection.

ACS Style

Daniel Hernandez-Patlan; Bruno Solis-Cruz; Bishnu Adhikari; Karine P. Pontin; Juan D. Latorre; Mikayla F.A. Baxter; Xochitl Hernandez-Velasco; Ruben Merino-Guzman; Abraham Méndez-Albores; Young Min Kwon; Billy M. Hargis; Raquel López-Arellano; Margarita A. Arreguin-Nava; Guillermo Tellez-Isaias. Evaluation of the antimicrobial and intestinal integrity properties of boric acid in broiler chickens infected with Salmonella enteritidis: Proof of concept. Research in Veterinary Science 2018, 123, 7 -13.

AMA Style

Daniel Hernandez-Patlan, Bruno Solis-Cruz, Bishnu Adhikari, Karine P. Pontin, Juan D. Latorre, Mikayla F.A. Baxter, Xochitl Hernandez-Velasco, Ruben Merino-Guzman, Abraham Méndez-Albores, Young Min Kwon, Billy M. Hargis, Raquel López-Arellano, Margarita A. Arreguin-Nava, Guillermo Tellez-Isaias. Evaluation of the antimicrobial and intestinal integrity properties of boric acid in broiler chickens infected with Salmonella enteritidis: Proof of concept. Research in Veterinary Science. 2018; 123 ():7-13.

Chicago/Turabian Style

Daniel Hernandez-Patlan; Bruno Solis-Cruz; Bishnu Adhikari; Karine P. Pontin; Juan D. Latorre; Mikayla F.A. Baxter; Xochitl Hernandez-Velasco; Ruben Merino-Guzman; Abraham Méndez-Albores; Young Min Kwon; Billy M. Hargis; Raquel López-Arellano; Margarita A. Arreguin-Nava; Guillermo Tellez-Isaias. 2018. "Evaluation of the antimicrobial and intestinal integrity properties of boric acid in broiler chickens infected with Salmonella enteritidis: Proof of concept." Research in Veterinary Science 123, no. : 7-13.

Journal article
Published: 21 November 2018 in Toxins
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Experiments were carried out to evaluate the effectiveness of three different biosorbents (banana peel, Pyracantha leaves, and Aloe powder) in removing aflatoxin B1 (AFB1). A noncommercial mycotoxin binder (zeolite) was used as a reference material. A laboratory model that simulated the in vivo conditions of the poultry gastrointestinal tract was utilized to prove the removal efficiency of the biosorbents when added to AFB1-contaminated diet (100 µg/kg). The concentration of AFB1 was determined using antibody-based immunoaffinity column and spectrofluorometry methodologies. Z potential (ζ), point of zero charge (pHpzc), energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy with attenuated total reflection (FTIR-ATR), and UV-Vis diffuse reflectance spectroscopy (DRS) techniques were used to further characterize the biosorbents. The addition of the biosorbents (1.5%, w/w) to the diet significantly reduced the bioavailability of AFB1 in the intestinal section. The highest aflatoxin adsorption values were 69% and 70% using Aloe powder and zeolite, respectively. A moderate biosorption uptake of 46% was achieved using Pyracantha leaves. The biomaterial with the lowest removal capacity was banana peel (28%). In conclusion, Aloe powder could be used as an alternative to conventional systems for AFB1 removal.

ACS Style

Anai Zavala-Franco; Daniel Hernández-Patlán; Bruno Solís-Cruz; Raquel López-Arellano; Guillermo Tellez-Isaias; Alma Vázquez-Durán; Abraham Méndez-Albores. Assessing the Aflatoxin B1 Adsorption Capacity between Biosorbents Using an In Vitro Multicompartmental Model Simulating the Dynamic Conditions in the Gastrointestinal Tract of Poultry. Toxins 2018, 10, 484 .

AMA Style

Anai Zavala-Franco, Daniel Hernández-Patlán, Bruno Solís-Cruz, Raquel López-Arellano, Guillermo Tellez-Isaias, Alma Vázquez-Durán, Abraham Méndez-Albores. Assessing the Aflatoxin B1 Adsorption Capacity between Biosorbents Using an In Vitro Multicompartmental Model Simulating the Dynamic Conditions in the Gastrointestinal Tract of Poultry. Toxins. 2018; 10 (11):484.

Chicago/Turabian Style

Anai Zavala-Franco; Daniel Hernández-Patlán; Bruno Solís-Cruz; Raquel López-Arellano; Guillermo Tellez-Isaias; Alma Vázquez-Durán; Abraham Méndez-Albores. 2018. "Assessing the Aflatoxin B1 Adsorption Capacity between Biosorbents Using an In Vitro Multicompartmental Model Simulating the Dynamic Conditions in the Gastrointestinal Tract of Poultry." Toxins 10, no. 11: 484.

Research article
Published: 22 August 2018 in Rapid Communications in Mass Spectrometry
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Rationale Isoprostane 8‐iso‐PGF2α is a biomarker of lipid peroxidation in cell membranes. The method developed to measure plasma total levels (esterified+ free) of 8‐iso‐PGF2α must be reproducible and be able to reduce the use of solvents in solid phase extraction. It should be useful to evaluate oxidative stress due to the excess of free radicals that are generated by some disorder or disease. Methods The method was developed using solid phase micro‐extraction with Oasis®MAX μElution plates and Ultra Performance Liquid Chromatography to tandem mass spectrometry (UPLC‐MS/MS). Electrospray ionization was performed in the negative mode (ESI‐); the multiple reaction monitoring mode (MRM) was used. The development of the method included the optimization of the chromatographic conditions to achieve the separation of PGF2α and 8‐iso‐PGF2α as well as the optimization of the microextraction conditions of the analyte of interest in ovine and goat plasma. Results The developed method was validated with a calibration curve of plasma samples fortified with standards at 5 concentration levels in a range of 49‐639 pg/mL. The average recovery was 89% with a standard deviation of 10.73%. The inter‐day precision was evaluated, obtaining a CV less than 15%. The limit of quantification was 20 pg/mL, and the limit of detection was 10 pg/mL. The 8‐iso‐PGF2a was determined in the plasma of 14 sheep and 20 goats of 5 months of age and 6 goats of 24 months of age. The concentrations found were 50 to 300 pg/mL. Conclusions The method developed is precise, accurate and reliable with low reagent consumption compared to conventional solid phase extraction. The analysis time was decreased because, with the use of the microextraction plate, the step of the evaporation and reconstitution of the analyte was avoided. The method is applicable to quantify the plasma total levels (esterified + free) of 8‐iso‐PGF2α.

ACS Style

Gabriela Rodríguez Patiño; Miriam Aide Castillo Rodríguez; Jacinto Efren Ramírez Bribiesca; Patricia Ramírez Noguera; María Eugenia Gonsebatt Bonaparte; Raquel López-Arellano; Rodríguez Patiño Gabriela; Castillo Rodríguez Miriam Aide; Ramírez Bribiesca Jacinto Efren; Ramírez Noguera Patricia; Gonsebatt Bonaparte María Eugenia; López Arellano Raquel. Development of a method for the determination of 8-iso-PGF2α in sheep and goat plasma using solid-phase microextraction and ultra-performance liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry 2018, 32, 1675 -1682.

AMA Style

Gabriela Rodríguez Patiño, Miriam Aide Castillo Rodríguez, Jacinto Efren Ramírez Bribiesca, Patricia Ramírez Noguera, María Eugenia Gonsebatt Bonaparte, Raquel López-Arellano, Rodríguez Patiño Gabriela, Castillo Rodríguez Miriam Aide, Ramírez Bribiesca Jacinto Efren, Ramírez Noguera Patricia, Gonsebatt Bonaparte María Eugenia, López Arellano Raquel. Development of a method for the determination of 8-iso-PGF2α in sheep and goat plasma using solid-phase microextraction and ultra-performance liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry. 2018; 32 (19):1675-1682.

Chicago/Turabian Style

Gabriela Rodríguez Patiño; Miriam Aide Castillo Rodríguez; Jacinto Efren Ramírez Bribiesca; Patricia Ramírez Noguera; María Eugenia Gonsebatt Bonaparte; Raquel López-Arellano; Rodríguez Patiño Gabriela; Castillo Rodríguez Miriam Aide; Ramírez Bribiesca Jacinto Efren; Ramírez Noguera Patricia; Gonsebatt Bonaparte María Eugenia; López Arellano Raquel. 2018. "Development of a method for the determination of 8-iso-PGF2α in sheep and goat plasma using solid-phase microextraction and ultra-performance liquid chromatography/tandem mass spectrometry." Rapid Communications in Mass Spectrometry 32, no. 19: 1675-1682.

Original article
Published: 14 August 2018 in Journal of Pharmaceutical Innovation
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Curcumin (CUR), a natural polyphenolic compound, has several pharmacological uses, primarily regarding its anti-inflammatory, chemotherapeutic, and antioxidant properties. However, to date, a significant drawback of curcumin is its poor bioavailability due to its low solubility and permeability. Therefore, the association of curcumin in polymeric nanocapsules may be an excellent strategy to increase its bioavailability. Two nanocapsule systems were developed with an oily core of vitamin E surrounded by a biodegradable polymeric shell of either chitosan (NC-CS) or alginate (NC-ALG) capable of improving the encapsulation efficiency, stability, and permeability of CUR. NC-CS and NC-ALG showed particle sizes of approximately 116.7 ± 3.2 and 178 ± 7.9 nm, dispersities of 0.107 and 0.149, and zeta potentials of 24.4 ± 2.1 and − 49.0 ± 2.3 mV, respectively. The encapsulation efficiency was approximately 90% in both cases, and they were demonstrated to be stable under storage conditions for 3 months. Cytotoxicity studies performed in Caco-2 cells using the method of trypan blue dye revealed that even at a high concentration of chitosan and alginate (157.9 μg/cm2 or 600 mg/mL), both of the nanocapsules were not toxic, exhibiting cell viability > 80%. The permeability was evaluated using Caco-2 cells as an in vitro model of the epithelial barrier. The obtained results show that the permeability of NC-CS and NC-ALG encapsulated CUR was considerably higher compared to that of an aqueous suspension. The obtained results suggest that nanocapsules could improve the solubility, permeability, and stability of curcumin.

ACS Style

Daniel Hernandez-Patlan; Bruno Solis-Cruz; Mario Alberto Cano-Vega; Eric Beyssac; Ghislain Garrait; Xochitl Hernandez-Velasco; Raquel López-Arellano; Guillermo Tellez; Gustavo R. Rivera-Rodriguez. Development of Chitosan and Alginate Nanocapsules to Increase the Solubility, Permeability and Stability of Curcumin. Journal of Pharmaceutical Innovation 2018, 14, 132 -140.

AMA Style

Daniel Hernandez-Patlan, Bruno Solis-Cruz, Mario Alberto Cano-Vega, Eric Beyssac, Ghislain Garrait, Xochitl Hernandez-Velasco, Raquel López-Arellano, Guillermo Tellez, Gustavo R. Rivera-Rodriguez. Development of Chitosan and Alginate Nanocapsules to Increase the Solubility, Permeability and Stability of Curcumin. Journal of Pharmaceutical Innovation. 2018; 14 (2):132-140.

Chicago/Turabian Style

Daniel Hernandez-Patlan; Bruno Solis-Cruz; Mario Alberto Cano-Vega; Eric Beyssac; Ghislain Garrait; Xochitl Hernandez-Velasco; Raquel López-Arellano; Guillermo Tellez; Gustavo R. Rivera-Rodriguez. 2018. "Development of Chitosan and Alginate Nanocapsules to Increase the Solubility, Permeability and Stability of Curcumin." Journal of Pharmaceutical Innovation 14, no. 2: 132-140.

Original research article
Published: 15 June 2018 in Frontiers in Microbiology
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In the present study, in vitro assays were conducted to evaluate the solubility of curcumin (CUR) alone or with polyvinylpyrrolidone (PVP) at different pH, as well as its permeability in Caco-2 cells. Results confirmed that the solid dispersion of CUR with PVP (CUR/PVP) at a 1:9 ratio, significantly increased (P < 0.05) solubility and permeability compared to CUR alone. Then, the antimicrobial activity of CUR/PVP, boric acid (BA), and a combination of 0.5% CUR/PVP and 0.5% BA (CUR/PVP-BA) against Salmonella Enteritidis (SE) was determined using an in vitro digestion model that simulates crop, proventriculus, and intestine. The results revealed that in the proventriculus and intestinal compartments significant reductions of SE were observed in all the experimental treatments, but 1% BA eliminated SE in the intestinal compartment and CUR/PVP-BA showed a synergistic effect on antimicrobial activity against SE. To complement these findings, two independent in vivo trials were conducted to determine the effect of 0.1% CUR/PVP; 0.1% BA; or the combination of 0.05% CUR/PVP (1:9 ratio) and 0.05% BA (CUR/PVP-BA) on the antimicrobial activity against SE, intestinal permeability and inflammatory responses in broiler chickens. BA at 0.1% had no significant in vivo effects against SE. However, the combination of 0.05% BA and 0.05% CUR/PVP and 0.05% BA was sufficient to reduce crop and intestinal SE colonization in broiler chickens in two independent trials, confirming the synergic effect between them. A similar antimicrobial impact against SE intestinal colonization was observed in chickens treated with 0.1% CUR/PVP at a 1:9 ratio, which could be due to the increase in solubility of CUR by PVP. Furthermore, 0.1% CUR/PVP reduced the intestinal permeability of FITC-d and total intestinal IgA, as well as increase the activity of SOD when compared to control, while, CUR/PVP-BA only decreased SOD activity. Further studies to confirm and expand the in vivo results obtained in this pilot study, adding intestinal microbial commensal groups and more inflammatory biomarkers to get a complete description of the effects of BA and CUR deserves further investigation.

ACS Style

Daniel Hernandez-Patlan; Bruno Solis-Cruz; Karine Patrin Pontin; Juan D Latorre; Mikayla F. A. Baxter; Xochitl Hernandez-Velasco; Ruben Merino-Guzman; Abraham Méndez-Albores; Billy M. Hargis; Raquel López-Arellano; Guillermo Tellez. Evaluation of a Solid Dispersion of Curcumin With Polyvinylpyrrolidone and Boric Acid Against Salmonella Enteritidis Infection and Intestinal Permeability in Broiler Chickens: A Pilot Study. Frontiers in Microbiology 2018, 9, 1289 .

AMA Style

Daniel Hernandez-Patlan, Bruno Solis-Cruz, Karine Patrin Pontin, Juan D Latorre, Mikayla F. A. Baxter, Xochitl Hernandez-Velasco, Ruben Merino-Guzman, Abraham Méndez-Albores, Billy M. Hargis, Raquel López-Arellano, Guillermo Tellez. Evaluation of a Solid Dispersion of Curcumin With Polyvinylpyrrolidone and Boric Acid Against Salmonella Enteritidis Infection and Intestinal Permeability in Broiler Chickens: A Pilot Study. Frontiers in Microbiology. 2018; 9 ():1289.

Chicago/Turabian Style

Daniel Hernandez-Patlan; Bruno Solis-Cruz; Karine Patrin Pontin; Juan D Latorre; Mikayla F. A. Baxter; Xochitl Hernandez-Velasco; Ruben Merino-Guzman; Abraham Méndez-Albores; Billy M. Hargis; Raquel López-Arellano; Guillermo Tellez. 2018. "Evaluation of a Solid Dispersion of Curcumin With Polyvinylpyrrolidone and Boric Acid Against Salmonella Enteritidis Infection and Intestinal Permeability in Broiler Chickens: A Pilot Study." Frontiers in Microbiology 9, no. : 1289.

Journal article
Published: 01 February 2018 in Ciencia y Agricultura
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Entre los principales problemas sanitarios para la producción ovina están los parásitos gastroentéricos; de ellos, el más importante es Haemonchus contortus. Las estrategias actuales de control enfocan la eficacia, junto con el bajo o nulo impacto al ecosistema; el estudio del control integral de parásitos permite la investigación de alternativas con probable efecto antihelmíntico, entre ellas el uso de semilla de papaya (Carica papaya). El objetivo de este trabajo fue evaluar el efecto in vitro de los extractos hidroalcohólico y etanólico de la semilla de papaya (C. papaya) sobre huevos y larvas de H. contortus. El trabajo se llevó a cabo con el equipo e instalaciones del Laboratorio 3 de la Unidad de Investigación Multidisciplinaria en la Facultad de Estudios Superiores Cuautitlán, UNAM. Se elaboraron los extractos por medio de maceración en solvente. Se inoculó un ovino con 5000 larvas de tercer estadio (L3) de H. contortus, cepa aislada y mantenida en dichas instalaciones. Se tomaron muestras de materia fecal y se recuperaron huevos para el ensayo de inhibición de la eclosión. Los huevos y las L3 fueron pretratados con extracto hidroalcohólico y etanólico de semilla de papaya (2.5 mg/ml), agua destilada, etanol 90 % y levamisol (7.5 mg/ml). Se efectuó la técnica de explantes abomasales, evaluando el efecto de los extractos sobre las L3 y su asociación a la mucosa abomasal. Se realizó un ANOVA para muestras repetidas por medio del programa Statgraphics. El extracto etanólico de semilla de papaya mostró el 92 % de inhibición de la eclosión; el extracto hidroalcohólico mostró 50 % (p<0.05). En el caso de los explantes, el extracto etanólico mostró el 10 % de larvas no asociadas al tejido abomasal, seguido por el extracto hidroalcohólico, con el 60 % de larvas no asociadas (p<0.05). Se concluye que el extracto etanólico tiene efecto sobre huevos, mientras que el extracto hidroalcohólico inhibe la asociación al tejido abomasal.

ACS Style

María De Los Ángeles Marroquín-Tun; Rosa Isabel Higuera-Piedrahita; María Eugenia López-Arellano; Raquel López-Arellano; Héctor Alejandro De la Cruz-Cruz; Rocío Silva-Mendoza; Jorge Alfredo Cuéllar-Ordaz. Efecto in vitro de los extractos hidroalcohólico y etanólico de semilla de papaya (Carica papaya) en Haemonchus contortus. Ciencia y Agricultura 2018, 15, 53 -59.

AMA Style

María De Los Ángeles Marroquín-Tun, Rosa Isabel Higuera-Piedrahita, María Eugenia López-Arellano, Raquel López-Arellano, Héctor Alejandro De la Cruz-Cruz, Rocío Silva-Mendoza, Jorge Alfredo Cuéllar-Ordaz. Efecto in vitro de los extractos hidroalcohólico y etanólico de semilla de papaya (Carica papaya) en Haemonchus contortus. Ciencia y Agricultura. 2018; 15 (1):53-59.

Chicago/Turabian Style

María De Los Ángeles Marroquín-Tun; Rosa Isabel Higuera-Piedrahita; María Eugenia López-Arellano; Raquel López-Arellano; Héctor Alejandro De la Cruz-Cruz; Rocío Silva-Mendoza; Jorge Alfredo Cuéllar-Ordaz. 2018. "Efecto in vitro de los extractos hidroalcohólico y etanólico de semilla de papaya (Carica papaya) en Haemonchus contortus." Ciencia y Agricultura 15, no. 1: 53-59.

Comparative study
Published: 08 January 2018 in Journal of Applied Microbiology
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AimsTo compare the conventional plating method versus a fluorometric method using PrestoBlue® as a dye by determining the antimicrobial activity of two organic acids and curcumin against Salmonella Enteritidis in an avian in vitro digestion model that simulates the crop, proventriculus and intestine.Methods and ResultsA concentration of 108 cfu ml−1 of S. Enteritidis was exposed to groups with different rates of ascorbic acid, boric acid and curcumin. Significant differences were observed when the means of the treatments were compared with the controls in the compartments that simulate the crop and intestine (P < 0.05). Ascorbic acid alone and high rates of ascorbic acid in the mixtures were the most efficient treatments in the crop compartment. However, in the intestinal compartment boric acid alone and at different rates in the mixture boric acid-curcumin (1:1) were the best treatments to decrease the concentration of S. Enteritidis.ConclusionsThe results of the present study suggest that there could be an antagonistic bactericidal effect between ascorbic acid-curcumin and ascorbic acid-boric acid as well as a synergistic bactericidal effect between boric acid-curcumin.Significance and Impact of StudyThese findings may contribute to the development of a formulation with microencapsulated compounds to liberate them in different compartments to combat S. Enteritidis infections in broiler chickens.This article is protected by copyright. All rights reserved.

ACS Style

Daniel Hernandez‐Patlan; Bruno Solis‐Cruz; Abraham Méndez‐Albores; Juan D. Latorre; Xochitl Hernandez‐Velasco; Guillermo Tellez; Raquel López-Arellano. Comparison of PrestoBlue® and plating method to evaluate antimicrobial activity of ascorbic acid, boric acid and curcumin in an in vitro gastrointestinal model. Journal of Applied Microbiology 2018, 124, 1 .

AMA Style

Daniel Hernandez‐Patlan, Bruno Solis‐Cruz, Abraham Méndez‐Albores, Juan D. Latorre, Xochitl Hernandez‐Velasco, Guillermo Tellez, Raquel López-Arellano. Comparison of PrestoBlue® and plating method to evaluate antimicrobial activity of ascorbic acid, boric acid and curcumin in an in vitro gastrointestinal model. Journal of Applied Microbiology. 2018; 124 (2):1.

Chicago/Turabian Style

Daniel Hernandez‐Patlan; Bruno Solis‐Cruz; Abraham Méndez‐Albores; Juan D. Latorre; Xochitl Hernandez‐Velasco; Guillermo Tellez; Raquel López-Arellano. 2018. "Comparison of PrestoBlue® and plating method to evaluate antimicrobial activity of ascorbic acid, boric acid and curcumin in an in vitro gastrointestinal model." Journal of Applied Microbiology 124, no. 2: 1.

Communication
Published: 19 October 2017 in Polymers
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Mycotoxins are secondary toxic metabolites that are produced by fungi representing threats to human and animal health. The objective of this study was to evaluate the adsorption capacity of Chitosan (CHI), and three cellulosic polymers (HPMC, CMC, and MCC), on six mycotoxins (AFB1; FUB1; OTA; T-2; DON; and, ZEA) using an in vitro digestive model for poultry. The adsorbent capacity of the materials in the supernatant of each compartment was evaluated by a non-competitive chemiluminescent assay. Control groups with no adsorbent material had an adsorption value of 0.00% against all six mycotoxins that were evaluated. All four materials tested showed significant (p < 0.05) binding activity against all of the mycotoxins when compared with the control non-treated group. However HPMC, CMC, and MCC showed better adsorbent capacity when compared with CHI.

ACS Style

Bruno Solís-Cruz; Daniel Hernández-Patlán; Eric Beyssac; Juan D. Latorre; Xochitl Hernandez-Velasco; Ruben Merino-Guzman; Guillermo Tellez; Raquel López-Arellano. Evaluation of Chitosan and Cellulosic Polymers as Binding Adsorbent Materials to Prevent Aflatoxin B1, Fumonisin B1, Ochratoxin, Trichothecene, Deoxynivalenol, and Zearalenone Mycotoxicoses Through an In Vitro Gastrointestinal Model for Poultry. Polymers 2017, 9, 529 .

AMA Style

Bruno Solís-Cruz, Daniel Hernández-Patlán, Eric Beyssac, Juan D. Latorre, Xochitl Hernandez-Velasco, Ruben Merino-Guzman, Guillermo Tellez, Raquel López-Arellano. Evaluation of Chitosan and Cellulosic Polymers as Binding Adsorbent Materials to Prevent Aflatoxin B1, Fumonisin B1, Ochratoxin, Trichothecene, Deoxynivalenol, and Zearalenone Mycotoxicoses Through an In Vitro Gastrointestinal Model for Poultry. Polymers. 2017; 9 (12):529.

Chicago/Turabian Style

Bruno Solís-Cruz; Daniel Hernández-Patlán; Eric Beyssac; Juan D. Latorre; Xochitl Hernandez-Velasco; Ruben Merino-Guzman; Guillermo Tellez; Raquel López-Arellano. 2017. "Evaluation of Chitosan and Cellulosic Polymers as Binding Adsorbent Materials to Prevent Aflatoxin B1, Fumonisin B1, Ochratoxin, Trichothecene, Deoxynivalenol, and Zearalenone Mycotoxicoses Through an In Vitro Gastrointestinal Model for Poultry." Polymers 9, no. 12: 529.

Journal article
Published: 12 October 2017 in Journal of the Mexican Chemical Society
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A reversed phase high-performance liquid chromatography (RP-HPLC) method for determination of Thimerosal (TMS) in topical creams was optimized and validated according to the ICH guidelines which include accuracy, precision, selectivity, robustness, limit of detection (LOD), limit of quantification (LOQ), linearity and range. For topical creams, sample treatment is often an overwhelming step essentially due to its oily nature. For the first time a simple and robust extraction procedure for TMS using phosphate buffer (pH 5.5, 0.2M) was successfully developed. This method describes the TMS quantitation by HPLC in a topical product containing 0.01% fluocinolone acetonide (FLA) as the active molecule. The HPLC separation was achieved on a Column Symmetry® and a methanol: phosphate buffer (pH 2.5, 0.05M) 70:30 v/v mobile phase and wavelength 218 nm. Results from both standards and samples showed adequate validation parameters. Noteworthy, linearity was within the range 1.2 - 2.8 μg/mL. Additionally, robustness and TMS stability were established after sample extraction. The method provides an efficient and safe quality control tool for determination of TMS in topical creams.

ACS Style

Guadalupe Pérez-Caballero; Nancy Muro-Hidalgo; Elvia Adriana Morales-Hipólito; Alma Villaseñor; Raquel López-Arellano. Development and ICH Validation of a RP-HPLC-UV Method for the Quantification of Thimerosal in Topic Creams. Journal of the Mexican Chemical Society 2017, 60, 1 .

AMA Style

Guadalupe Pérez-Caballero, Nancy Muro-Hidalgo, Elvia Adriana Morales-Hipólito, Alma Villaseñor, Raquel López-Arellano. Development and ICH Validation of a RP-HPLC-UV Method for the Quantification of Thimerosal in Topic Creams. Journal of the Mexican Chemical Society. 2017; 60 (4):1.

Chicago/Turabian Style

Guadalupe Pérez-Caballero; Nancy Muro-Hidalgo; Elvia Adriana Morales-Hipólito; Alma Villaseñor; Raquel López-Arellano. 2017. "Development and ICH Validation of a RP-HPLC-UV Method for the Quantification of Thimerosal in Topic Creams." Journal of the Mexican Chemical Society 60, no. 4: 1.

Journal article
Published: 22 June 2017 in Small Ruminant Research
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Selenium (Se) prevents oxidative damage and stimulates the immune system. Currently, there are no data available evaluating the Se-induced antigenic response to Mannheimia haemolytica and oxidative stress in goat kids. Twenty-one 6-month-old male Alpine Goat kids (22.9 kg) were immunized against M. haemolytica and divided into 3 groups: Basal diet with no additional Se (CG); Se injected subcutaneously at 0.25 mg Se/kg live weight (LW) (SeSG); and Se administered as intraruminal bolus at 0.46 mg Se/kg LW (SeBG). Blood samples were taken from the animals in all groups at 0, 14, 28, 42 and 56 days post-dosing. Erythrocyte Se levels doubled in SeSG during days 14–28 post-dosing (130.6 ng/g Se) as compared to CG. During days 28–42 post-dosing, Se levels decreased (P < 0.05) in SeSG (106.9 ng/g Se) vs. CG and SeBG (86.2 ng/g Se and 81.7 ng/g Se, respectively). Glutathione (GSH) levels decreased in days 0–28 post-dosing (3.7E−4 nmols/mg of total protein) and then, remained stable up to day 56 (5.6E−4 nmols/mg of total protein). Catalase levels in the SeBG and SeSG (79.9 U) were higher (P < 0.05) than in the CG (56.3 U) by day 14 post-dosing. Malondialdehyde (MDA) levels on day 14 post-dosing increased 7-fold in the SeBG (1.28E−3 ng/mg of total protein). IgG levels increased in the groups treated with Se during days 28–42 post-dosing (1.4 nM) vs. the CG (0.10 nM) (P < 0.05). Treatment with Se improved the immunological response to M. haemolytica.

ACS Style

Víctor Manuel Díaz Sánchez; Gabriela Rodríguez-Patiño; Patricia Ramírez-Noguera; J. Efren Ramirez-Bribiesca; José F. Morales-Álvarez; Alma Luisa Revilla Vázquez; Raquel López-Arellano. Dose of selenium in goat kids and its effect on the antigenic response to Mannheimia haemolytica and oxidative stress. Small Ruminant Research 2017, 153, 171 -174.

AMA Style

Víctor Manuel Díaz Sánchez, Gabriela Rodríguez-Patiño, Patricia Ramírez-Noguera, J. Efren Ramirez-Bribiesca, José F. Morales-Álvarez, Alma Luisa Revilla Vázquez, Raquel López-Arellano. Dose of selenium in goat kids and its effect on the antigenic response to Mannheimia haemolytica and oxidative stress. Small Ruminant Research. 2017; 153 ():171-174.

Chicago/Turabian Style

Víctor Manuel Díaz Sánchez; Gabriela Rodríguez-Patiño; Patricia Ramírez-Noguera; J. Efren Ramirez-Bribiesca; José F. Morales-Álvarez; Alma Luisa Revilla Vázquez; Raquel López-Arellano. 2017. "Dose of selenium in goat kids and its effect on the antigenic response to Mannheimia haemolytica and oxidative stress." Small Ruminant Research 153, no. : 171-174.