Mycotoxins are secondary toxic metabolites that are produced by fungi representing threats to human and animal health. The objective of this study was to evaluate the adsorption capacity of Chitosan (CHI), and three cellulosic polymers (HPMC, CMC, and MCC), on six mycotoxins (AFB₁; FUB₁; OTA; T-2; DON; and, ZEA) using an in vitro digestive model for poultry. The adsorbent capacity of the materials in the supernatant of each compartment was evaluated by a non-competitive chemiluminescent assay. Control groups with no adsorbent material had an adsorption value of 0.00% against all six mycotoxins that were evaluated. All four materials tested showed significant (p < 0.05) binding activity against all of the mycotoxins when compared with the control non-treated group. However HPMC, CMC, and MCC showed better adsorbent capacity when compared with CHI.

Standard compendia dissolution apparatus are the first choice for development of new dissolution methods. Nevertheless, limitations coming from the amount of material available, analytical sensitivity, lack of discrimination or biorelevance may warrant the use of non compendial methods. In this regard, the use of small volume dissolution methods offers strong advantages. The present study aims primarily to evaluate the dissolution performance of various drug products having different release mechanisms, using commercially available small volume USP2 dissolution equipment. The present series of tests indicate that the small volume dissolution is a useful tool for the characterization of immediate release drug product. Depending on the release mechanism, different speed factors are proposed to mimic common one liter vessel performance. In addition, by increasing the discriminating power of the dissolution method, it potentially improves know how about formulations and on typical events which are evaluated during pharmaceutical development such as ageing or scale–up. In this regard, small volume dissolution is a method of choice in case of screening for critical quality attributes of rapidly dissolving tablets, where it is often difficult to detect differences using standard working conditions.