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The combination of natural products with standard chemotherapeutic agents offers a promising strategy to enhance the efficacy or reduce the side effects of standard chemotherapy. Doxorubicin (DOX), a standard drug for breast cancer, has several disadvantages, including severe side effects and the development of drug resistance. Recently, we reported the potential bioactive markers of Australian propolis extract (AP-1) and their broad spectrum of pharmacological activities. In the present study, we explored the synergistic interactions between AP-1 and DOX in the MCF7 breast adenocarcinoma cells using different synergy quantitation models. Biochemometric and metabolomics-driven analysis was performed to identify the potential anticancer metabolites in AP-1. The molecular mechanisms of synergy were studied by analysing the apoptotic profile via flow cytometry, apoptotic proteome array and measuring the oxidative status of the MCF7 cells treated with the most synergistic combination. Furthermore, label-free quantification proteomics analysis was performed to decipher the underlying synergistic mechanisms. Five prenylated stilbenes were identified as the key metabolites in the most active AP-1 fraction. Strong synergy was observed when AP-1 was combined with DOX in the ratio of 100:0.29 (w/w) as validated by different synergy quantitation models implemented. AP-1 significantly enhanced the inhibitory effect of DOX against MCF7 cell proliferation in a dose-dependent manner with significant inhibition of the reactive oxygen species (p< 0.0001) compared to DOX alone. AP-1 enabled the reversal of DOX-mediated necrosis to programmed cell death, which may be advantageous to decline DOX-related side effects. AP-1 also significantly enhanced the apoptotic effect of DOX after 24 h of treatment with significant upregulation of catalase, HTRA2/Omi, FADD together with DR5 and DR4 TRAIL-mediated apoptosis (p< 0.05), contributing to the antiproliferative activity of AP-1. Significant upregulation of pro-apoptotic p27, PON2 and catalase with downregulated anti-apoptotic XIAP, HSP60 and HIF-1α, and increased antioxidant proteins (catalase and PON2) may be associated with the improved apoptosis and oxidative status of the synergistic combination-treated MCF7 cells compared to the mono treatments. Shotgun proteomics identified 21 significantly dysregulated proteins in the synergistic combination-treated cells versus the mono treatments. These proteins were involved in the TP53/ATM-regulated non-homologous end-joining pathway and double-strand breaks repairs, recruiting the overexpressed BRCA1 and suppressed RIF1 encoded proteins. The overexpression of UPF2 was noticed in the synergistic combination treatment, which could assist in overcoming doxorubicin resistance-associated long non-coding RNA and metastasis of the MCF7 cells. In conclusion, we identified the significant synergy and highlighted the key molecular pathways in the interaction between AP-1 and DOX in the MCF7 cells together with the AP-1 anticancer metabolites. Further in vivo and clinical studies are warranted on this synergistic combination.
Muhammad Alsherbiny; Deep Bhuyan; Ibrahim Radwan; Dennis Chang; Chun-Guang Li. Metabolomic Identification of Anticancer Metabolites of Australian Propolis and Proteomic Elucidation of Its Synergistic Mechanisms with Doxorubicin in the MCF7 Cells. International Journal of Molecular Sciences 2021, 22, 7840 .
AMA StyleMuhammad Alsherbiny, Deep Bhuyan, Ibrahim Radwan, Dennis Chang, Chun-Guang Li. Metabolomic Identification of Anticancer Metabolites of Australian Propolis and Proteomic Elucidation of Its Synergistic Mechanisms with Doxorubicin in the MCF7 Cells. International Journal of Molecular Sciences. 2021; 22 (15):7840.
Chicago/Turabian StyleMuhammad Alsherbiny; Deep Bhuyan; Ibrahim Radwan; Dennis Chang; Chun-Guang Li. 2021. "Metabolomic Identification of Anticancer Metabolites of Australian Propolis and Proteomic Elucidation of Its Synergistic Mechanisms with Doxorubicin in the MCF7 Cells." International Journal of Molecular Sciences 22, no. 15: 7840.
Background Complementary remedies such as the Chinese herb ‘Sheng Ma’ (Black cohosh; Actaea racemosa ‘AR’) are being sought to overcome the shortcomings of conventional hormonal and surgical therapies developed for the treatment of polycystic ovary syndrome (PCOS). However, AR-induced hepatotoxicity necessitates a cautionary warning to be labeled on its products as recommended by the United States Pharmacopeia, where four out of seven hepatotoxic cases in Sweden were possibly associated with black cohosh products. Methods We investigated the effects, safety, and molecular targets of black cohosh ethanolic extract and/or vitamin C on ovarian functionality and oxidative response in hyperandrogenism-induced PCOS rats. A well-established rat model using oral letrozole, daily, for 21 days was employed. The rats then received the AR extract with and without vitamin C for 28 days. The hormonal evaluation, antioxidant status, histopathological examination, immunohistochemical analysis, cell proliferation, and the expression ratio of the aromatase (Cyp19α1) gene were evaluated. Additionally, holistic profiling of the AR arsenal of secondary metabolites was performed using ultra-high-performance liquid chromatography (UHPLC) coupled with quadrupole high-resolution time of flight mass spectrometry (QTOF-MS). Results Beneficial effects were exerted by AR in PCOS rats as antioxidant status, hormonal profile, lipid profile, glucose level, liver functions, and the induced Ki-67 expression in the granulosa, theca cell layers and interstitial stromal cells were all improved. Notably, the combination of AR with vitamin C was not only more effective in reversing the dysregulated levels of testosterone, luteinizing hormone, and mRNA level of Cyp19α1 gene in the PCOS rat, but also safer. The combination regulated both ovarian and hepatic malondialdehyde (MDA) and glutathione (GSH) levels with histological improvement observed in the liver and ovaries. In addition, the untargeted metabolomic profiling enabled the identification of 61 metabolites allocated in five major chemical classes. Conclusion This study demonstrated the benefit of the combinatorial effects of AR and vitamin C in mitigating the reproductive and metabolic disorders associated with PCOS with the elimination of AR hepatotoxic risk.
Asmaa A. Azouz; Sara E. Ali; Reham M. Abd-Elsalam; Shimaa R. Emam; Mona K. Galal; Sherif H. Elmosalamy; Muhammed A. Alsherbiny; Bardes B. Hassan; Chun Guang Li; Shymaa A. El Badawy. Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in letrozole induced polycystic ovarian syndrome rat model: promising activity without the risk of hepatic adverse effect. Chinese Medicine 2021, 16, 1 -17.
AMA StyleAsmaa A. Azouz, Sara E. Ali, Reham M. Abd-Elsalam, Shimaa R. Emam, Mona K. Galal, Sherif H. Elmosalamy, Muhammed A. Alsherbiny, Bardes B. Hassan, Chun Guang Li, Shymaa A. El Badawy. Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in letrozole induced polycystic ovarian syndrome rat model: promising activity without the risk of hepatic adverse effect. Chinese Medicine. 2021; 16 (1):1-17.
Chicago/Turabian StyleAsmaa A. Azouz; Sara E. Ali; Reham M. Abd-Elsalam; Shimaa R. Emam; Mona K. Galal; Sherif H. Elmosalamy; Muhammed A. Alsherbiny; Bardes B. Hassan; Chun Guang Li; Shymaa A. El Badawy. 2021. "Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in letrozole induced polycystic ovarian syndrome rat model: promising activity without the risk of hepatic adverse effect." Chinese Medicine 16, no. 1: 1-17.
Background Acrylamide (ACR) is a widespread industrial and food contaminant that garnered considerable attention for its carcinogenic, neurotoxic, and reproductive toxic effects. The antioxidant effects of Portulaca oleracea seeds extract (POS) and its fertility-enhancing effects were inspiring to evaluate the protective potential and pinpoint the mechanisms and molecular targets of the UPLC-MS fingerprinted POS extract on ACR-induced testicular toxicity in rats. Methods Male Wistar rats were divided into 6 equal groups of negative control, ACR model (10 mg/kg b.wt.), POS at doses of (200 and 400 mg/kg b.wt.) and POS-treated ACR groups. All treatments were given by oral dosing every day for 60 days. Results Administration of POS extract reversed the ACR-induced epididymides weight loss with improved semen quality and count, ameliorated the ACR-decreased testicular lesion scoring, testicular oxidative stress, testicular degeneration, Leydig cell apoptosis and the dysregulated PCNA and Caspase-3 expression in a dose-dependent manner. It upregulated the declined level of serum testosterone and the expression of steroidogenic genes such as CYP11A1 and 17β3-HSD with an obvious histologic improvement of the testes with re-establishment of the normal spermatogenic series, Sertoli and Leydig cells. Conclusions The supplementation with POS extract may provide a potential protective effect for ACR-induced testicular dysfunction which is mediated by its antioxidant, antiapoptotic and steroidogenic modulatory effects.
Ola M. Farag; Reham M. Abd-Elsalam; Shymaa A. El Badawy; Hanan A. Ogaly; Muhammad A. Alsherbiny; Kawkab A. Ahmed. Portulaca oleracea seeds’ extract alleviates acrylamide-induced testicular dysfunction by promoting oxidative status and steroidogenic pathway in rats. BMC Complementary Medicine and Therapies 2021, 21, 1 -15.
AMA StyleOla M. Farag, Reham M. Abd-Elsalam, Shymaa A. El Badawy, Hanan A. Ogaly, Muhammad A. Alsherbiny, Kawkab A. Ahmed. Portulaca oleracea seeds’ extract alleviates acrylamide-induced testicular dysfunction by promoting oxidative status and steroidogenic pathway in rats. BMC Complementary Medicine and Therapies. 2021; 21 (1):1-15.
Chicago/Turabian StyleOla M. Farag; Reham M. Abd-Elsalam; Shymaa A. El Badawy; Hanan A. Ogaly; Muhammad A. Alsherbiny; Kawkab A. Ahmed. 2021. "Portulaca oleracea seeds’ extract alleviates acrylamide-induced testicular dysfunction by promoting oxidative status and steroidogenic pathway in rats." BMC Complementary Medicine and Therapies 21, no. 1: 1-15.
Acrylamide (ACR) is a widespread industrial and food contaminant that garnered considerable attention for its carcinogenic, neurotoxic, and reproductive toxic effects. The antioxidant effects of Portulaca oleracea seeds extract (POS) and its fertility-enhancing effects were inspiring to evaluate the protective potential and pinpoint the mechanisms and molecular targets of the UPLC-MS fingerprinted POS extract on ACR-induced testicular toxicity in rats. Male Wistar rats were divided into 6 equal groups of negative control, ACR model (10 mg/kg b.wt.), POS at doses of (200 and 400 mg/kg b.wt.) and POS-treated ACR groups. All treatments were given by oral dosing every day for 60 days. Administration of POS extract reversed the ACR-induced epididymides weight loss with improved semen quality and count, ameliorated the ACR-decreased testicular lesion scoring, testicular oxidative stress, testicular degeneration, Leydig cell apoptosis and the dysregulated PCNA and Caspase-3 expression in a dose-dependent manner. It upregulated the declined level of serum testosterone and the expression of steroidogenic genes such as CYP11A1 and 17β3-HSD with an obvious histologic improvement of the testes with re-establishment of the normal spermatogenic series, Sertoli and Leydig cells. The supplementation with POS extract may provide a potential protective effect for ACR-induced testicular dysfunction which is mediated by its antioxidant, antiapoptotic and steroidogenic modulatory effects. The online version contains supplementary material available at 10.1186/s12906-021-03286-2.
Ola M. Farag; Reham M. Abd-Elsalam; Shymaa A. El Badawy; Hanan A. Ogaly; Muhammad A. Alsherbiny; Kawkab A. Ahmed. Portulaca oleracea seeds’ extract alleviates acrylamide-induced testicular dysfunction by promoting oxidative status and steroidogenic pathway in rats. 2021, 21, 122 .
AMA StyleOla M. Farag, Reham M. Abd-Elsalam, Shymaa A. El Badawy, Hanan A. Ogaly, Muhammad A. Alsherbiny, Kawkab A. Ahmed. Portulaca oleracea seeds’ extract alleviates acrylamide-induced testicular dysfunction by promoting oxidative status and steroidogenic pathway in rats. . 2021; 21 (1):122.
Chicago/Turabian StyleOla M. Farag; Reham M. Abd-Elsalam; Shymaa A. El Badawy; Hanan A. Ogaly; Muhammad A. Alsherbiny; Kawkab A. Ahmed. 2021. "Portulaca oleracea seeds’ extract alleviates acrylamide-induced testicular dysfunction by promoting oxidative status and steroidogenic pathway in rats." 21, no. 1: 122.
The broad-spectrum pharmacological activity of Australian propolis and identification of key markers of propolis samples from Australia, Brazil and China.
Deep Jyoti Bhuyan; Muhammad A. Alsherbiny; Mitchell Nolan Low; Xian Zhou; Kirandeep Kaur; George Li; Chun Guang Li. Broad-spectrum pharmacological activity of Australian propolis and metabolomic-driven identification of marker metabolites of propolis samples from three continents. Food & Function 2021, 1 .
AMA StyleDeep Jyoti Bhuyan, Muhammad A. Alsherbiny, Mitchell Nolan Low, Xian Zhou, Kirandeep Kaur, George Li, Chun Guang Li. Broad-spectrum pharmacological activity of Australian propolis and metabolomic-driven identification of marker metabolites of propolis samples from three continents. Food & Function. 2021; ():1.
Chicago/Turabian StyleDeep Jyoti Bhuyan; Muhammad A. Alsherbiny; Mitchell Nolan Low; Xian Zhou; Kirandeep Kaur; George Li; Chun Guang Li. 2021. "Broad-spectrum pharmacological activity of Australian propolis and metabolomic-driven identification of marker metabolites of propolis samples from three continents." Food & Function , no. : 1.
Acrylamide (ACR) is an environmental pollutant with well-demonstrated neurotoxic and neurodegenerative effects in both humans and experimental animals. The present study aimed to investigate the neuroprotective effect of Portulaca oleracea seeds extract (PSE) against ACR-induced neurotoxicity in rats and its possible underlying mechanisms. PSE was subjected to phytochemical investigation using ultra-high-performance liquid chromatography (UPLC) coupled with quantitative time of flight mass spectrometry (qTOF-MS). Multivariate, clustering and correlation data analyses were performed to assess the overall effects of PSE on ACR-challenged rats. Rats were divided into six groups including negative control, ACR-intoxicated group (10 mg/kg/day), PSE treated groups (200 and 400 mg/kg/day), and ACR + PSE treated groups (200 and 400 mg/kg/day, respectively). All treatments were given intragastrically for 60 days. PSE markedly ameliorated brain damage as evidenced by the decreased lactate dehydrogenase (LDL), increased acetylcholinesterase (AchE) activities, as well as the increased brain‐derived neurotrophic factor (BDNF) that were altered by the toxic dose of ACR. In addition, PSE markedly attenuated ACR-induced histopathological alterations in the cerebrum, cerebellum, hippocampus and sciatic nerve and downregulated the ACR-inclined GFAP expression. PSE restored the oxidative status in the brain as indicated by glutathione (GSH), lipid peroxidation and increased total antioxidant capacity (TAC). PSE upregulated the mRNA expression of protein kinase B (AKT), which resulted in an upsurge in its downstream cAMP response element-binding protein (CREB)/BDNF mRNA expression in the brain tissue of ACR-intoxicated rats. All exerted PSE beneficial effects were dose-dependent, with the ACR-challenged group received PSE 400 mg/kg dose showed a close clustering to the negative control in both unsupervised principal component analysis (PCA) and supervised orthogonal partial least square discriminant analysis (OPLS-Da) alongside with the hierarchical clustering analysis (HCA). The current investigation confirmed the neuroprotective capacity of PSE against ACR-induced brain injury, and our findings indicate that AKT/CREB pathways and BDNF synthesis may play an important role in the PSE-mediated protective effects against ACR-triggered neurotoxicity.
Ola M. Farag; Reham M. Abd-Elsalam; Hanan A. Ogaly; Sara E. Ali; Shymaa A. El Badawy; Muhammed A. Alsherbiny; Chun Guang Li; Kawkab A. Ahmed. Metabolomic Profiling and Neuroprotective Effects of Purslane Seeds Extract Against Acrylamide Toxicity in Rat’s Brain. Neurochemical Research 2021, 46, 819 -842.
AMA StyleOla M. Farag, Reham M. Abd-Elsalam, Hanan A. Ogaly, Sara E. Ali, Shymaa A. El Badawy, Muhammed A. Alsherbiny, Chun Guang Li, Kawkab A. Ahmed. Metabolomic Profiling and Neuroprotective Effects of Purslane Seeds Extract Against Acrylamide Toxicity in Rat’s Brain. Neurochemical Research. 2021; 46 (4):819-842.
Chicago/Turabian StyleOla M. Farag; Reham M. Abd-Elsalam; Hanan A. Ogaly; Sara E. Ali; Shymaa A. El Badawy; Muhammed A. Alsherbiny; Chun Guang Li; Kawkab A. Ahmed. 2021. "Metabolomic Profiling and Neuroprotective Effects of Purslane Seeds Extract Against Acrylamide Toxicity in Rat’s Brain." Neurochemical Research 46, no. 4: 819-842.
Background: Complementary remedies such as the Chinese herb ‘Sheng Ma’ (Black cohosh; Actaea racemosa ‘AR’) are being sought to overcome the shortcomings of conventional hormonal and surgical therapies developed for treatment of polycystic ovary syndrome (PCOS). However, the AR hepatotoxicity urges a cautionary warning to be labeled on its products as recommended by the United States Pharmacopeia, where four out of seven hepatotoxic cases in Sweden were possibly associated with black cohosh products.Methods: We investigated the effects, safety, and molecular targets of black cohosh ethanolic extract and/or vitamin C on ovarian functionality and oxidative response in hyperandrogenism-induced PCOS rats. A well-established rat model using oral letrozole, daily, for 21 days was employed then rats received the AR extract with and without vitamin C for 28 days. The hormonal evaluation, antioxidant status, histopathological examination, immunohistochemical analysis, cell proliferation, and the expression ratio of the aromatase (Cyp19α1) gene were evaluated. Additionally, a holistic profiling of AR arsenal of secondary metabolites was performed using ultra high-performance liquid chromatography (UHPLC) coupled with quadrupole high resolution time of flight mass spectrometry (QTOF-MS).Results: Beneficial effects were exerted by AR in PCOS rats via the improved antioxidant status, hormonal profile, lipid profile, glucose level, liver functions, and the induced Ki-67 expression in the granulosa, theca cell layers and interstitial stromal cells. Notably, the combination of AR with vitamin C was not only more effective in reversing the dysregulated levels of testosterone, luteinizing hormone, and mRNA level of Cyp19α1 gene in the PCOS rat, but also safer. The combination regulated both ovarian and hepatic MDA and GSH levels with histological improvement observed in the liver and ovaries. In addition, the untargeted metabolomic profiling enabled the identification of 61 metabolites allocated in five major chemical classes.Conclusion: This study demonstrated the benefit of the combination between AR and vitamin C in mitigating the reproductive and metabolic disorders of PCOS with the elimination of AR hepatotoxic risk.
Asmaa A. Azouz; Sara E. Ali; Reham M. Abd-Elsalam; Shimaa R. Emam; Mona K. Galal; Sherif H. Elmosalamy; Muhammad Alsherbiny; Bardes B. Hassan; Chun Guang Li; Shymaa A. El Badawy. Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in letrozole induced polycystic ovarian syndrome rat model: Promising activity without the risk of hepatic adverse effect. 2020, 1 .
AMA StyleAsmaa A. Azouz, Sara E. Ali, Reham M. Abd-Elsalam, Shimaa R. Emam, Mona K. Galal, Sherif H. Elmosalamy, Muhammad Alsherbiny, Bardes B. Hassan, Chun Guang Li, Shymaa A. El Badawy. Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in letrozole induced polycystic ovarian syndrome rat model: Promising activity without the risk of hepatic adverse effect. . 2020; ():1.
Chicago/Turabian StyleAsmaa A. Azouz; Sara E. Ali; Reham M. Abd-Elsalam; Shimaa R. Emam; Mona K. Galal; Sherif H. Elmosalamy; Muhammad Alsherbiny; Bardes B. Hassan; Chun Guang Li; Shymaa A. El Badawy. 2020. "Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in letrozole induced polycystic ovarian syndrome rat model: Promising activity without the risk of hepatic adverse effect." , no. : 1.
Background Complementary remedies such as the Chinese herb ‘Sheng Ma’ (Black cohosh; Actaea racemose ‘AR’) are being sought to surmount the shortcomings of conventional hormonal and surgical therapies developed in the treatment of polycystic ovary syndrome (PCOS). However, the AR hepatotoxicity urges a cautionary warning to be labelled on its products as recommended by the United States Pharmacopeia where 4 out of 7 hepatotoxic cases in Sweden were possibly associated with black cohosh products.Methods We investigated the efficacy, safety and molecular targets of black cohosh ethanolic extract and/or vitamin C on ovarian functionality, oxidative response in the hyperandrogenism-induced PCOS alongside with holistic profiling of its arsenal of secondary metabolites using UPLC-Qtof-MS. A well-established rat model using oral letrozole, daily, for 21 days was employed then the rats received the AR extract with and without vitamin C for 28 days. The hormonal evaluation, antioxidant status, histopathological examination, immunohistochemical analysis, cell proliferation, apoptosis, and the expression ratio of the aromatase (Cyp19α1) gene were evaluated.Results Beneficial effects were exerted by AR in PCOS rats via the improved antioxidant status, hormonal profile, lipid profile, glucose level, liver functions, curtailed percentage of apoptotic cells and the induced Ki-67 expression in the granulosa, theca cell layers and interstitial stromal cells. Notably, AR combination with vitamin C was not only more effective to reverse the dysregulated levels of testosterone, luteinising hormone, and mRNA level of Cyp19α1 gene in the PCOS rat, but also safer, while the combination regulated both ovarian and hepatic MDA and GSH levels with a histologic improvement noticed in livers and ovaries. In addition, the untargeted metabolomic profiling enabled the identification of 61 metabolites allocated in five major chemical classes.Conclusion This study demonstrated the benefit of the combination between AR and vitamin C in mitigating the reproductive and metabolic disorders of PCOS with the elimination of AR hepatotoxic risk.
Asmaa A. Azouz; Sara E. Ali; Reham M. Abd-Elsalam; Shimaa R. Emam; Mona K. Galal; Sherif H. Elmosalamy; Muhammad Alsherbiny; Bardes B. Hassan; Chun Guang Li; Shymaa A. El Badawy. Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in Letrozole induced polycystic ovarian syndrome rat model: Promising activity without the risk of hepatic adverse effect. 2020, 1 .
AMA StyleAsmaa A. Azouz, Sara E. Ali, Reham M. Abd-Elsalam, Shimaa R. Emam, Mona K. Galal, Sherif H. Elmosalamy, Muhammad Alsherbiny, Bardes B. Hassan, Chun Guang Li, Shymaa A. El Badawy. Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in Letrozole induced polycystic ovarian syndrome rat model: Promising activity without the risk of hepatic adverse effect. . 2020; ():1.
Chicago/Turabian StyleAsmaa A. Azouz; Sara E. Ali; Reham M. Abd-Elsalam; Shimaa R. Emam; Mona K. Galal; Sherif H. Elmosalamy; Muhammad Alsherbiny; Bardes B. Hassan; Chun Guang Li; Shymaa A. El Badawy. 2020. "Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in Letrozole induced polycystic ovarian syndrome rat model: Promising activity without the risk of hepatic adverse effect." , no. : 1.
Convergent and convenient regioselective synthesis of novel thiazolo[2,3-a]pyrimidines was accomplished using the one-pot reaction of 6-ethylthiouracil, bromoacetic acid, anhydrous sodium acetate, acetic anhydride, acetic acid and suitable aldehyde.
Mohamed Fares; Patrick M. McCosker; Muhammad A. Alsherbiny; Anthony C. Willis; Timothy Clark; Johan Neyts; Dirk Jochmans; Paul A. Keller. Regioselective convergent synthesis of 2-arylidene thiazolo[3,2-a]pyrimidines as potential anti-chikungunya agents. RSC Advances 2020, 10, 5191 -5195.
AMA StyleMohamed Fares, Patrick M. McCosker, Muhammad A. Alsherbiny, Anthony C. Willis, Timothy Clark, Johan Neyts, Dirk Jochmans, Paul A. Keller. Regioselective convergent synthesis of 2-arylidene thiazolo[3,2-a]pyrimidines as potential anti-chikungunya agents. RSC Advances. 2020; 10 (9):5191-5195.
Chicago/Turabian StyleMohamed Fares; Patrick M. McCosker; Muhammad A. Alsherbiny; Anthony C. Willis; Timothy Clark; Johan Neyts; Dirk Jochmans; Paul A. Keller. 2020. "Regioselective convergent synthesis of 2-arylidene thiazolo[3,2-a]pyrimidines as potential anti-chikungunya agents." RSC Advances 10, no. 9: 5191-5195.
Persea americana, commonly known as avocado, has recently gained substantial popularity and is often marketed as a “superfood” because of its unique nutritional composition, antioxidant content, and biochemical profile. However, the term “superfood” can be vague and misleading, as it is often associated with unrealistic health claims. This review draws a comprehensive summary and assessment of research performed in the last few decades to understand the nutritional and therapeutic properties of avocado and its bioactive compounds. In particular, studies reporting the major metabolites of avocado, their antioxidant as well as bioavailability and pharmacokinetic properties, are summarized and assessed. Furthermore, the potential of avocado in novel drug discovery for the prevention and treatment of cancer, microbial, inflammatory, diabetes, and cardiovascular diseases is highlighted. This review also proposes several interesting future directions for avocado research.
Deep Jyoti Bhuyan; Muhammad A. Alsherbiny; Saumya Perera; Mitchell Low; Amrita Basu; Okram Abemsana Devi; Mridula Saikia Barooah; Chun Guang Li; Konstantinos Papoutsis; Low; Basu; Devi; Li. The Odyssey of Bioactive Compounds in Avocado (Persea americana) and their Health Benefits. Antioxidants 2019, 8, 426 .
AMA StyleDeep Jyoti Bhuyan, Muhammad A. Alsherbiny, Saumya Perera, Mitchell Low, Amrita Basu, Okram Abemsana Devi, Mridula Saikia Barooah, Chun Guang Li, Konstantinos Papoutsis, Low, Basu, Devi, Li. The Odyssey of Bioactive Compounds in Avocado (Persea americana) and their Health Benefits. Antioxidants. 2019; 8 (10):426.
Chicago/Turabian StyleDeep Jyoti Bhuyan; Muhammad A. Alsherbiny; Saumya Perera; Mitchell Low; Amrita Basu; Okram Abemsana Devi; Mridula Saikia Barooah; Chun Guang Li; Konstantinos Papoutsis; Low; Basu; Devi; Li. 2019. "The Odyssey of Bioactive Compounds in Avocado (Persea americana) and their Health Benefits." Antioxidants 8, no. 10: 426.
The endocannabinoids system (ECS) has garnered considerable interest as a potential therapeutic target in various carcinomas and cancer-related conditions alongside neurodegenerative diseases. Cannabinoids are implemented in several physiological processes such as appetite stimulation, energy balance, pain modulation and the control of chemotherapy-induced nausea and vomiting (CINV). However, pharmacokinetics and pharmacodynamics interactions could be perceived in drug combinations, so in this short review we tried to shed light on the potential drug interactions of medicinal cannabis. Hitherto, few data have been provided to the healthcare practitioners about the drug–drug interactions of cannabinoids with other prescription medications. In general, cannabinoids are usually well tolerated, but bidirectional effects may be expected with concomitant administered agents via affected membrane transporters (Glycoprotein p, breast cancer resistance proteins, and multidrug resistance proteins) and metabolizing enzymes (Cytochrome P450 and UDP-glucuronosyltransferases). Caution should be undertaken to closely monitor the responses of cannabis users with certain drugs to guard their safety, especially for the elderly and people with chronic diseases or kidney and liver conditions.
Muhammad A. Alsherbiny; Chun Guang Li. Medicinal Cannabis—Potential Drug Interactions. Medicines 2018, 6, 3 .
AMA StyleMuhammad A. Alsherbiny, Chun Guang Li. Medicinal Cannabis—Potential Drug Interactions. Medicines. 2018; 6 (1):3.
Chicago/Turabian StyleMuhammad A. Alsherbiny; Chun Guang Li. 2018. "Medicinal Cannabis—Potential Drug Interactions." Medicines 6, no. 1: 3.
Endocannbinoids system (ECS) engrossed a considerable interest as potential therapeutic targets in various carcinomas and cancer related conditions alongside with neurodegenerative diseases. Cannabinoids are implemented in several physiological processes such as appetite stimulation, energy balance, pain modulation and the control of chemotherapy induced nausea and vomiting (CINV). However, pharmacokinetics and pharmacodynamics interactions could be perceived in drug combinations, so in this short review we tried to shed the light over the potential drug interactions of medicinal cannabis. Hitherto, few data have been provided to the healthcare practitioners about the drug-drug interactions of cannabinoids with other prescription medications. In general, cannabinoids are usually well tolerated, but the bidirectional effects may be expected with concomitant administered agents via affected membrane transporters (glycoprotein p, breast cancer resistance proteins) and metabolizing enzymes (Cytochrome P450 and UDP- glucuronosyltransferases). The caveats should be undertaken to closely monitor the responses of cannabis users with certain drugs to guard their safety, especially for the elderly and people with chronic diseases or kidney and liver conditions.
Muhammad A. Alsherbiny; Chun G. Li. Medicinal Cannabis - Potential Drug Interactions. 2018, 1 .
AMA StyleMuhammad A. Alsherbiny, Chun G. Li. Medicinal Cannabis - Potential Drug Interactions. . 2018; ():1.
Chicago/Turabian StyleMuhammad A. Alsherbiny; Chun G. Li. 2018. "Medicinal Cannabis - Potential Drug Interactions." , no. : 1.
Fatal unintentional poisoning is widespread upon human exposure to toxic agents such as pesticides, heavy metals, environmental pollutants, bacterial and fungal toxins or even some medications and cosmetic products. In this regards, the application of the natural dietary agents as antidotes has engrossed a substantial attention. One of the ancient known traditional medicines and spices with an arsenal of metabolites of several reported health benefits is ginger. This extended literature review serves to demonstrate the protective effects and mechanisms of ginger and its phytochemicals against natural, chemical and radiation-induced toxicities. Collected data obtained from the in-vivo and in-vitro experimental studies in this overview detail the designation of the protective effects to ginger's antioxidant, anti-inflammatory, and anti-apoptotic properties. Ginger's armoury of phytochemicals exerted its protective function via different mechanisms and cell signalling pathways, including Nrf2/ARE, MAPK, NF-ƙB, Wnt/β-catenin, TGF-β1/Smad3, and ERK/CREB. The outcomes of this review could encourage further clinical trials of ginger applications in radiotherapy and chemotherapy regime for cancer treatments or its implementation to counteract the chemical toxicity induced by industrial pollutants, alcohol, smoking or administered drugs.
Muhammad A. Alsherbiny; Wessam H. Abd-Elsalam; Shymaa A. El Badawy; Ehab Taher; Mohamed Fares; Allan Torres; Dennis Chang; Chun Guang Li. Ameliorative and protective effects of ginger and its main constituents against natural, chemical and radiation-induced toxicities: A comprehensive review. Food and Chemical Toxicology 2018, 123, 72 -97.
AMA StyleMuhammad A. Alsherbiny, Wessam H. Abd-Elsalam, Shymaa A. El Badawy, Ehab Taher, Mohamed Fares, Allan Torres, Dennis Chang, Chun Guang Li. Ameliorative and protective effects of ginger and its main constituents against natural, chemical and radiation-induced toxicities: A comprehensive review. Food and Chemical Toxicology. 2018; 123 ():72-97.
Chicago/Turabian StyleMuhammad A. Alsherbiny; Wessam H. Abd-Elsalam; Shymaa A. El Badawy; Ehab Taher; Mohamed Fares; Allan Torres; Dennis Chang; Chun Guang Li. 2018. "Ameliorative and protective effects of ginger and its main constituents against natural, chemical and radiation-induced toxicities: A comprehensive review." Food and Chemical Toxicology 123, no. : 72-97.
In our endeavor towards the development of potent anticancer agents, two different sets of biphenylurea-indolinone conjugates, 5a–s and 8a,b were synthesized. The in vitro cytotoxicity of the synthesized compounds was examined in two human cancer cell lines, namely MCF-7 breast cancer and PC-3 prostate cancer cells using the sulforhodamine B (SRB) colorimetric assay. In particular, the MCF-7 cancer cell line was more susceptible to the synthesized compounds. Compound 5o (IC50 = 1.04 ± 0.10 μM) emerged as the most active member in this study against MCF-7, with 7-fold increased activity compared to the reference drug, doxorubicin (IC50 = 7.30 ± 0.84 μM). Compounds 5l, 5q and 8b also exhibited superior cytotoxic activity against MCF-7 with IC50 values of 1.93 ± 0.17, 3.87 ± 0.31 and 4.66 ± 0.42 μM, respectively. All of the tested compounds were filtered according to the Lipinski and Veber rules and all of them passed the filters. Additionally, several ADME descriptors for the synthesized compounds 5a–s and 8a,b were predicted via a theoretical kinetic study performed using the Discovery Studio 2.5 software.
Wagdy M. Eldehna; Mohamed Fares; Hany S. Ibrahim; Muhammad A. Alsherbiny; Mohamed H. Aly; Hazem A. Ghabbour; Hatem A. Abdel-Aziz. Synthesis and Cytotoxic Activity of Biphenylurea Derivatives Containing Indolin-2-one Moieties. Molecules 2016, 21, 762 .
AMA StyleWagdy M. Eldehna, Mohamed Fares, Hany S. Ibrahim, Muhammad A. Alsherbiny, Mohamed H. Aly, Hazem A. Ghabbour, Hatem A. Abdel-Aziz. Synthesis and Cytotoxic Activity of Biphenylurea Derivatives Containing Indolin-2-one Moieties. Molecules. 2016; 21 (6):762.
Chicago/Turabian StyleWagdy M. Eldehna; Mohamed Fares; Hany S. Ibrahim; Muhammad A. Alsherbiny; Mohamed H. Aly; Hazem A. Ghabbour; Hatem A. Abdel-Aziz. 2016. "Synthesis and Cytotoxic Activity of Biphenylurea Derivatives Containing Indolin-2-one Moieties." Molecules 21, no. 6: 762.
We reported herein the synthesis, antifungal activity, docking and in silico ADME prediction studies of four novel series of sulfones 6a–f, 8a–c, 10a–f and 12a–c. All the newly synthesized sulfones were tested against four strains of Candida (including fluconazole-resistant Candida), two strains of Aspergillus, two dermatophytic fungi (Trichophytons mentagrophyte and Microsporum canis) and Syncephalastrum sp. with fluconazole as a reference drug. In general, compounds 8a and 10b showed selective and potent anticandidal activity (MIC: 0.19–0.81 µM) relative to fluconazole (MIC = 1.00 µM). Furthermore, 10e and 12a elicited a remarkable and selective antifungal activity against Aspergillus sp. and the dermatophytic fungi (MIC: 0.16–0.79 µM) relative to fluconazole (MIC: 2–2.6 µM). Moreover, the docking results of the sulfones 6a, 8a, 10a and 10b at the active site of CYT P450 14α-sterol demethylase showed a comparable binding interaction (interaction Energy = −34.87 to −42.43 kcal/mol) with that of fluconazole (IE = −40.37 kcal/mol).
Mohamed Fares; Mohamed A. Said; Muhammad A. Alsherbiny; Radwa A. Eladwy; Hadia Almahli; Marwa M. Abdel-Aziz; Hazem A. Ghabbour; Wagdy M. Eldehna; Hatem A. Abdel-Aziz. Synthesis, Biological Evaluation and Molecular Docking of Certain Sulfones as Potential Nonazole Antifungal Agents. Molecules 2016, 21, 114 .
AMA StyleMohamed Fares, Mohamed A. Said, Muhammad A. Alsherbiny, Radwa A. Eladwy, Hadia Almahli, Marwa M. Abdel-Aziz, Hazem A. Ghabbour, Wagdy M. Eldehna, Hatem A. Abdel-Aziz. Synthesis, Biological Evaluation and Molecular Docking of Certain Sulfones as Potential Nonazole Antifungal Agents. Molecules. 2016; 21 (1):114.
Chicago/Turabian StyleMohamed Fares; Mohamed A. Said; Muhammad A. Alsherbiny; Radwa A. Eladwy; Hadia Almahli; Marwa M. Abdel-Aziz; Hazem A. Ghabbour; Wagdy M. Eldehna; Hatem A. Abdel-Aziz. 2016. "Synthesis, Biological Evaluation and Molecular Docking of Certain Sulfones as Potential Nonazole Antifungal Agents." Molecules 21, no. 1: 114.
The present study was conducted to evaluate both the cytotoxic and anti-inflammatory activities of ethanol extracts (T), and both n-butanol (B) and total glyco-alkaloid fractions (TGA) of Solanum seaforthianum Andr. (SS) and Solanum macrocarpon L. (SM) growing in Egypt. Cytotoxic activity was measured using sulforhodamine B (SRB) assay on prostate cancer cell line (PC-3), breast cancer cell line (MCF7), liver cancer cell line (HepG2) and human fibroblast cell line (HFB4) while anti-inflammatory activity was measured using formalin induced paw edema method. The highest cytotoxic potentiality was indicated for those of TGA fraction of S. seaforthianum Andr. on PC-3 cell line (IC50 = 0.28µg/ml ± 0.01) followed by its activity on MCF-7 cell line (IC50 = 2.84 µg/ml±0.20). On the other hand, the potency of TGA fractions of both species showed higher potency followed by n-butanol fractions where ethanol extracts showed lowest potency which is emphasizing the cytotoxic potentiality of the glyco-alkaloids. Based on the IC50s indicated for the different extracts and fractions on normal fibroblast cell line, considerable safety was indicated against prostate carcinoma rather than breast or hepatic carcinoma. TGA fraction of S. macrocarpon L. and of S. seaforthianum Andr. showed the highest anti-inflammatory activity with efficacy of 159 and 156%, respectively as compared to standard indomethacin. That’s why the TGA fraction of S. seaforthianum Andr. was subjected for isolation of individual alkaloids using different chromatographic techniques and identified using 1H and 13-CNMR spectroscopy beside Co-chromatography with authentic samples as solamargine (A1), solasonine (A2) and solasodine (A3) which are firstly isolated from S. seaforthianum Andr. growing in Egypt. Key words: Solanum seaforthianum, Solanum macrocarpon, glyco-alkaloid, anti-inflammatory, cytotoxicity, SRB.
A Alsherbiny Muhammad; M Ezzat Shahira; S Elsakhawy Fatma; M Kamel Gehan; A Abdel Kawy Mostafa; Muhammad A. Alsherbiny; Shahira M. Ezzat; Fatma S. Elsakhawy; Gehan M. Kamel; Mostafa A. Abdel-Kawy. Impact of certain Solanum speciess natural products as potent cytotoxic and anti-Inflammatory agents. Journal of Medicinal Plants Research 2015, 9, 779 -786.
AMA StyleA Alsherbiny Muhammad, M Ezzat Shahira, S Elsakhawy Fatma, M Kamel Gehan, A Abdel Kawy Mostafa, Muhammad A. Alsherbiny, Shahira M. Ezzat, Fatma S. Elsakhawy, Gehan M. Kamel, Mostafa A. Abdel-Kawy. Impact of certain Solanum speciess natural products as potent cytotoxic and anti-Inflammatory agents. Journal of Medicinal Plants Research. 2015; 9 (29):779-786.
Chicago/Turabian StyleA Alsherbiny Muhammad; M Ezzat Shahira; S Elsakhawy Fatma; M Kamel Gehan; A Abdel Kawy Mostafa; Muhammad A. Alsherbiny; Shahira M. Ezzat; Fatma S. Elsakhawy; Gehan M. Kamel; Mostafa A. Abdel-Kawy. 2015. "Impact of certain Solanum speciess natural products as potent cytotoxic and anti-Inflammatory agents." Journal of Medicinal Plants Research 9, no. 29: 779-786.
The current study evaluated the molluscicidal and schistosomicidal activities of different extracts and fractions of two Solanum species. The glycoalkaloids content depicted a promising activity against both the snails and the adult worms. Abbreviations Used: PZQ; Praziquantel, SM; Solanum macrocarpon, SS; Solanum seaforthianum, TGA; total glycoalkaloid.
Muhammad A. Alsherbiny; Shymaa El Badawy; Hesham Elbedewy; Shahira M. Ezzat; Fatma S. Elsakhawy; Mostafa A. Abdel-Kawy. Comparative Molluscicidal and Schistosomicidal Potentiality of Two Solanum Species and Its Isolated Glycoalkaloids. Pharmacognosy Research 2021, 10, 113 -117.
AMA StyleMuhammad A. Alsherbiny, Shymaa El Badawy, Hesham Elbedewy, Shahira M. Ezzat, Fatma S. Elsakhawy, Mostafa A. Abdel-Kawy. Comparative Molluscicidal and Schistosomicidal Potentiality of Two Solanum Species and Its Isolated Glycoalkaloids. Pharmacognosy Research. 2021; 10 (1):113-117.
Chicago/Turabian StyleMuhammad A. Alsherbiny; Shymaa El Badawy; Hesham Elbedewy; Shahira M. Ezzat; Fatma S. Elsakhawy; Mostafa A. Abdel-Kawy. 2021. "Comparative Molluscicidal and Schistosomicidal Potentiality of Two Solanum Species and Its Isolated Glycoalkaloids." Pharmacognosy Research 10, no. 1: 113-117.