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The broad-spectrum pharmacological activity of Australian propolis and identification of key markers of propolis samples from Australia, Brazil and China.
Deep Jyoti Bhuyan; Muhammad A. Alsherbiny; Mitchell Nolan Low; Xian Zhou; Kirandeep Kaur; George Li; Chun Guang Li. Broad-spectrum pharmacological activity of Australian propolis and metabolomic-driven identification of marker metabolites of propolis samples from three continents. Food & Function 2021, 1 .
AMA StyleDeep Jyoti Bhuyan, Muhammad A. Alsherbiny, Mitchell Nolan Low, Xian Zhou, Kirandeep Kaur, George Li, Chun Guang Li. Broad-spectrum pharmacological activity of Australian propolis and metabolomic-driven identification of marker metabolites of propolis samples from three continents. Food & Function. 2021; ():1.
Chicago/Turabian StyleDeep Jyoti Bhuyan; Muhammad A. Alsherbiny; Mitchell Nolan Low; Xian Zhou; Kirandeep Kaur; George Li; Chun Guang Li. 2021. "Broad-spectrum pharmacological activity of Australian propolis and metabolomic-driven identification of marker metabolites of propolis samples from three continents." Food & Function , no. : 1.
Marian Jarouche; Martha Ibrahim; Jarryd Pearson; Mitchell Low; James Rowe. Stability of Metronidazole Free-base Oral Suspensions Formulated with United States Pharmacopeia-grade Metronidazole Powder and Commercial Metronidazole Tablets. 2020, 24, 77 -82.
AMA StyleMarian Jarouche, Martha Ibrahim, Jarryd Pearson, Mitchell Low, James Rowe. Stability of Metronidazole Free-base Oral Suspensions Formulated with United States Pharmacopeia-grade Metronidazole Powder and Commercial Metronidazole Tablets. . 2020; 24 (1):77-82.
Chicago/Turabian StyleMarian Jarouche; Martha Ibrahim; Jarryd Pearson; Mitchell Low; James Rowe. 2020. "Stability of Metronidazole Free-base Oral Suspensions Formulated with United States Pharmacopeia-grade Metronidazole Powder and Commercial Metronidazole Tablets." 24, no. 1: 77-82.
Persea americana, commonly known as avocado, has recently gained substantial popularity and is often marketed as a “superfood” because of its unique nutritional composition, antioxidant content, and biochemical profile. However, the term “superfood” can be vague and misleading, as it is often associated with unrealistic health claims. This review draws a comprehensive summary and assessment of research performed in the last few decades to understand the nutritional and therapeutic properties of avocado and its bioactive compounds. In particular, studies reporting the major metabolites of avocado, their antioxidant as well as bioavailability and pharmacokinetic properties, are summarized and assessed. Furthermore, the potential of avocado in novel drug discovery for the prevention and treatment of cancer, microbial, inflammatory, diabetes, and cardiovascular diseases is highlighted. This review also proposes several interesting future directions for avocado research.
Deep Jyoti Bhuyan; Muhammad A. Alsherbiny; Saumya Perera; Mitchell Low; Amrita Basu; Okram Abemsana Devi; Mridula Saikia Barooah; Chun Guang Li; Konstantinos Papoutsis; Low; Basu; Devi; Li. The Odyssey of Bioactive Compounds in Avocado (Persea americana) and their Health Benefits. Antioxidants 2019, 8, 426 .
AMA StyleDeep Jyoti Bhuyan, Muhammad A. Alsherbiny, Saumya Perera, Mitchell Low, Amrita Basu, Okram Abemsana Devi, Mridula Saikia Barooah, Chun Guang Li, Konstantinos Papoutsis, Low, Basu, Devi, Li. The Odyssey of Bioactive Compounds in Avocado (Persea americana) and their Health Benefits. Antioxidants. 2019; 8 (10):426.
Chicago/Turabian StyleDeep Jyoti Bhuyan; Muhammad A. Alsherbiny; Saumya Perera; Mitchell Low; Amrita Basu; Okram Abemsana Devi; Mridula Saikia Barooah; Chun Guang Li; Konstantinos Papoutsis; Low; Basu; Devi; Li. 2019. "The Odyssey of Bioactive Compounds in Avocado (Persea americana) and their Health Benefits." Antioxidants 8, no. 10: 426.
Background-The quality control (QC) for commercial herbal formulations is sparse due to a lack of well-developed HPLC-ESI-MS/MS methods. Objective-This study reports the quantification of nine selected analytes for a commercial eight-herb formulation known as Qi Ju Di Huang Wan (QJDHW) used to relieve hypertension. Methods-An HPLC-ESI/MS method for the quantitation of analytes selected using the Herbal Chemical Marker Ranking System (Herb MaRS) was developed. The Herb MaRS ranking system which takes into account bioavailability, bioactivity, and physiological action related to its intended use and the commercial availability of the standard. After a method optimization, seven analytes were found to be ideal for quantitation. Results-The target analytes were identified using an electrospray ionization-tandem MS molecular breakdown comparison between the herbal peak and the commercial standard. The quantitative aspect of analyte variability of eleven samples was studied using fold variation. The fold variation of selected analytes among eleven samples ranged from 1.5 to 28.9. The qualitative aspect of variability was studied using principal component analysis (PCA) and hierarchical cluster analysis (HCA). Conclusions-There is a great degree of chemical variability in herbal formulations which are due to raw material harvesting times, storage techniques, and plant subspecies variability. Highlights-Commercial QJDHW formulations need to be standardised using HPLC-ESI-MS/MS to ensure better product quality control (QC) and product efficacy for the consumer.
Mariam Jarouche; Harsha Suresh; Mitchell Low; Cindy Xu; Cheang Khoo; Low; Xu; Khoo; Samiuela Lee. Quality Control and Variability Assessment of an Eight-Herb Formulation for Hypertension Using Method Validation and Statistical Analysis. Molecules 2019, 24, 1520 .
AMA StyleMariam Jarouche, Harsha Suresh, Mitchell Low, Cindy Xu, Cheang Khoo, Low, Xu, Khoo, Samiuela Lee. Quality Control and Variability Assessment of an Eight-Herb Formulation for Hypertension Using Method Validation and Statistical Analysis. Molecules. 2019; 24 (8):1520.
Chicago/Turabian StyleMariam Jarouche; Harsha Suresh; Mitchell Low; Cindy Xu; Cheang Khoo; Low; Xu; Khoo; Samiuela Lee. 2019. "Quality Control and Variability Assessment of an Eight-Herb Formulation for Hypertension Using Method Validation and Statistical Analysis." Molecules 24, no. 8: 1520.
The Naoxinqing (NXQ) tablet is a standardised proprietary herbal product containing an extract of persimmon leaves (Diospyros kaki) for the management of cardio- and cerebrovascular diseases. Although previous reports suggested that the efficacy of NXQ is at least partly mediated by its anti-oxidative property, the anti-oxidative effect of the major components of NXQ has not been studied systematically. For quality control purposes, only analytical methods limited to 3 marker analytes have been reported, the extent to which the other components affect efficacy has not been explored. In this study, we developed an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC MS/MS) method for the identification of seven analytes (kaempferol-3-O-glucoside (astragalin), quercetin-3-O-galactoside (hypericin), quercetin-3-O-glucoside (isoquercitin), kaempferol, 3,4-dihydroxybenzoic acid (protocatechuic acid), and furan-2-carboxylic acid (pyromucic acid) and quercetin) in the NXQ. This is the first method reported and validated for the quantification of the seven major secondary metabolites in NXQ. The results for the quantified analytes were then compared in 15 different batches of NXQ. The variation observed in the seven components highlights the need to quantify key bioactive components to ensure product consistency. Radical scavenging activity and abundance was used to rank the analytes. The anti-oxidative effects of NXQ were examined using cultured human vascular endothelial cells (EA.hy926). Corrected 2,2-di(4-tert-octylphenyl)-1-picrylhydrazyl (DPPH) activity results revealed that quercetin and kaempferol have the strongest anti-oxidant capacity in the extract. Both quercetin and kaempferol significantly inhibited the hydrogen peroxide (H₂O₂)-induced EA.hy926 cell injury and intracellular reactive oxygen species (ROS) generation. In conclusion, we established and validated an UPLC-MS/MC method for the analysis of major bioactive components in the NXQ and demonstrated that its anti-oxidative property may play a critical role in cerebrovascular protection.
Magdy Kazzem; Yu-Ting Sun; Mitchell Low; Sai Wang Seto; Dennis Chang; Samiuela Lee; Harsha Suresh; Cheang S. Khoo; Alan Bensoussan; Hosen Kiat. Chromatographic Analysis and Anti-Oxidative Property of Naoxinqing Tablet, a Proprietary Preparation of Diospyros Kaki Leaves. Molecules 2019, 24, 1101 .
AMA StyleMagdy Kazzem, Yu-Ting Sun, Mitchell Low, Sai Wang Seto, Dennis Chang, Samiuela Lee, Harsha Suresh, Cheang S. Khoo, Alan Bensoussan, Hosen Kiat. Chromatographic Analysis and Anti-Oxidative Property of Naoxinqing Tablet, a Proprietary Preparation of Diospyros Kaki Leaves. Molecules. 2019; 24 (6):1101.
Chicago/Turabian StyleMagdy Kazzem; Yu-Ting Sun; Mitchell Low; Sai Wang Seto; Dennis Chang; Samiuela Lee; Harsha Suresh; Cheang S. Khoo; Alan Bensoussan; Hosen Kiat. 2019. "Chromatographic Analysis and Anti-Oxidative Property of Naoxinqing Tablet, a Proprietary Preparation of Diospyros Kaki Leaves." Molecules 24, no. 6: 1101.
Chronic inflammation is an important pathological condition in many human diseases, and due to the side effects of the currently used non-steroidal anti-inflammatory drugs, discovery of novel anti-inflammatory drugs is of general interest. Anti-inflammatory activity guided compound isolation from the plant Alphitonia petriei led to the isolation of the known plant sterols emmolic acid (1), alphitolic acid (2), trans- and cis-coumaroyl esters of alphitolic acid (3 and 4) and betulinic acid (5). A detailed spectroscopic analysis led to the structure elucidation of the alphitolic acid derivatives (1–5), and the semi-synthetic emmolic acid acetate (6). When tested in LPS (Lipopolysaccharides) + IFN-γ (Interferon gamma) activated RAW 264.7 macrophages, all compounds except (1) exhibited potent anti-inflammatory activity (IC50 values as low as 1.7 μM) in terms of downregulation of NO and TNF-α production, but also demonstrated some considerable cytotoxicity.
Ritesh Raju; Dhanushka Gunawardena; Most Afia Ahktar; Mitchell Low; Paul Reddell; Gerald Münch. Anti-Inflammatory Chemical Profiling of the Australian Rainforest Tree Alphitonia petriei (Rhamnaceae). Molecules 2016, 21, 1521 .
AMA StyleRitesh Raju, Dhanushka Gunawardena, Most Afia Ahktar, Mitchell Low, Paul Reddell, Gerald Münch. Anti-Inflammatory Chemical Profiling of the Australian Rainforest Tree Alphitonia petriei (Rhamnaceae). Molecules. 2016; 21 (11):1521.
Chicago/Turabian StyleRitesh Raju; Dhanushka Gunawardena; Most Afia Ahktar; Mitchell Low; Paul Reddell; Gerald Münch. 2016. "Anti-Inflammatory Chemical Profiling of the Australian Rainforest Tree Alphitonia petriei (Rhamnaceae)." Molecules 21, no. 11: 1521.
Aims. This study aims to investigate the possible synergistic interactions of the Danshen-Sanqi combination on vascular disease via their anti-inflammatory activities.Methods. Nine combination ratios of Danshen-Sanqi extracts were screened in the RAW264.7 cell line and their anti-inflammatory effects were examined in lipopolysaccharide- (LPS-) induced nitric oxide (NO), tumor necrosis factor (TNF), and monocyte chemoattractant protein-1 (MCP-1) generation pathways. The interaction between Danshen and Sanqi on each target was analysed using combination index (CI) and isobologram models. Additionally, the anti-inflammatory activities of key bioactive compounds from Danshen and Sanqi were tested using the same models. The compounds from each herb that exerted the most potent activity were combined to evaluate their possible synergistic/antagonistic interactions.Results. Danshen-Sanqi 8 : 2 was found to be the optimal ratio and exerted a synergistic effect in inhibiting NO, TNF, and MCP-1 when the concentrations were higher than 1.24, 1.89, and 2.17 mg/mL, respectively. Although dihydrotanshinone I (DT) and ginsenoside Rd (Rd) from Danshen and Sanqi, respectively, exhibited the greatest individual bioactivity in the assays, antagonistic effects were observed for the DT-Rd combination 7 : 3.Conclusion. This study provided scientific evidence to support the traditional use of the Danshen-Sanqi combination for vascular disease through their synergistic interactions on anti-inflammatory pathways.
Xian Zhou; Valentina Razmovski-Naumovski; Dennis Chang; Chunguang Li; Antony Kam; Mitchell Low; Alan Bensoussan; Kelvin Chan. Synergistic Effects of Danshen (Salvia Miltiorrhiza Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) Combination in Inhibiting Inflammation Mediators in RAW264.7 Cells. BioMed Research International 2016, 2016, 1 -12.
AMA StyleXian Zhou, Valentina Razmovski-Naumovski, Dennis Chang, Chunguang Li, Antony Kam, Mitchell Low, Alan Bensoussan, Kelvin Chan. Synergistic Effects of Danshen (Salvia Miltiorrhiza Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) Combination in Inhibiting Inflammation Mediators in RAW264.7 Cells. BioMed Research International. 2016; 2016 ():1-12.
Chicago/Turabian StyleXian Zhou; Valentina Razmovski-Naumovski; Dennis Chang; Chunguang Li; Antony Kam; Mitchell Low; Alan Bensoussan; Kelvin Chan. 2016. "Synergistic Effects of Danshen (Salvia Miltiorrhiza Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) Combination in Inhibiting Inflammation Mediators in RAW264.7 Cells." BioMed Research International 2016, no. : 1-12.
The anti-inflammatory activity of Andrographis paniculata (Acanthaceae), a traditional medicine widely used in Asia, is commonly attributed to andrographolide, its main secondary metabolite. Commercial A. paniculata extracts are standardised to andrographolide content. We undertook the present study to investigate 1) how selective enrichment of andrographolide in commercial A. paniculata extracts affects the variability of non-standardised phytochemical components and 2) if variability in the non-standardised components of the extract affects the pharmacological activity of andrographolide itself. We characterized 12 commercial, standardised (≥30% andrographolide) batches of A. paniculata extracts from India by HPLC profiling. We determined the antioxidant capacity of the extracts using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, oxygen radical antioxidant capacity (ORAC) and a Folin-Ciocalteu (FC) antioxidant assays. Their anti-inflammatory activity was assessed by assaying their inhibitory effect on the release of tumor necrosis factor alpha (TNF-α) in the human monocytic cell line THP-1. The andrographolide content in the samples was close to the claimed value (32.2 ± 2.1%, range 27.5 to 35.9%). Twenty-one non-standardised constituents exhibited more than 2-fold variation in HPLC peak intensities in the tested batches. The chlorogenic acid content of the batches varied more than 30-fold. The DPPH free radical scavenging activity varied ~3-fold, the ORAC and FC antioxidant capacity varied ~1.5 fold among batches. In contrast, the TNF-α inhibitory activity of the extracts exhibited little variation and comparison with pure andrographolide indicated that it was mostly due to their andrographolide content. Standardised A. paniculata extracts contained the claimed amount of andrographolide but exhibited considerable phytochemical background variation. DPPH radical scavenging activity of the extracts was mostly due to the flavonoid/phenlycarboxylic acid compounds in the extracts. The inhibitory effect of andrographolide on the release of TNF-α was little affected by the quantitative variation of the non-standardised constituents.
Mitchell Low; Cheang S Khoo; Gerald Münch; Suresh Govindaraghavan; Nikolaus J Sucher. An in vitro study of anti-inflammatory activity of standardised Andrographis paniculata extracts and pure andrographolide. BMC Complementary and Alternative Medicine 2015, 15, 18 -18.
AMA StyleMitchell Low, Cheang S Khoo, Gerald Münch, Suresh Govindaraghavan, Nikolaus J Sucher. An in vitro study of anti-inflammatory activity of standardised Andrographis paniculata extracts and pure andrographolide. BMC Complementary and Alternative Medicine. 2015; 15 (1):18-18.
Chicago/Turabian StyleMitchell Low; Cheang S Khoo; Gerald Münch; Suresh Govindaraghavan; Nikolaus J Sucher. 2015. "An in vitro study of anti-inflammatory activity of standardised Andrographis paniculata extracts and pure andrographolide." BMC Complementary and Alternative Medicine 15, no. 1: 18-18.
A validated analytical method is reported for the analysis of chlorogenic and caffeic acids in Xu Duan (Dipsacus asperoides) in the dried raw herb. The ground samples were extracted by ultrasonication in water and the extract was analysed by LC-PDA with identity confirmation by (+)ESI-MS/MS. A C18 column was used with a 0.1% aqueous formic acid : methanol gradient mobile phase. The analytes were quantified 325 nm. With the MS detector, using the chlorogenic acid precursor ion with m/z 354, ions with m/z 191, and 85 were produced. For caffeic acid the precursor ion with m/z 181, ions with m/z 163, 135, and 89 were produced. The amount of chlorogenic and caffeic acids in the raw herb was found to be 4.46 and 0.63 mg/g, respectively, and the method LOD was 0.13 and 0.02 mg/g, respectively.
Jarryd L. Pearson; Samiuela Lee; Harsha Suresh; Mitchell Low; Marnilar Nang; Swastika Singh; Franklin Lamin; Magdy Kazzem; Shaun Sullivan; Cheang S. Khoo. The Liquid Chromatographic Determination of Chlorogenic and Caffeic Acids in Xu Duan (Dipsacus asperoides) Raw Herb. ISRN Analytical Chemistry 2014, 2014, 1 -6.
AMA StyleJarryd L. Pearson, Samiuela Lee, Harsha Suresh, Mitchell Low, Marnilar Nang, Swastika Singh, Franklin Lamin, Magdy Kazzem, Shaun Sullivan, Cheang S. Khoo. The Liquid Chromatographic Determination of Chlorogenic and Caffeic Acids in Xu Duan (Dipsacus asperoides) Raw Herb. ISRN Analytical Chemistry. 2014; 2014 ():1-6.
Chicago/Turabian StyleJarryd L. Pearson; Samiuela Lee; Harsha Suresh; Mitchell Low; Marnilar Nang; Swastika Singh; Franklin Lamin; Magdy Kazzem; Shaun Sullivan; Cheang S. Khoo. 2014. "The Liquid Chromatographic Determination of Chlorogenic and Caffeic Acids in Xu Duan (Dipsacus asperoides) Raw Herb." ISRN Analytical Chemistry 2014, no. : 1-6.
Chronic inflammatory processes contribute to the pathogenesis of many age-related diseases. In search of anti-inflammatory foods, we have systematically screened a variety of common dietary plants and mushrooms for their anti-inflammatory activity. A selection of 115 samples was prepared by a generic food-compatible processing method involving heating. These products were tested for their anti-inflammatory activity in murine N11 microglia and RAW 264.7 macrophages, using nitric oxide (NO) and tumour necrosis factor-α (TNF-α) as pro-inflammatory readouts. Ten food samples including lime zest, English breakfast tea, honey-brown mushroom, button mushroom, oyster mushroom, cinnamon and cloves inhibited NO production in N11 microglia, with IC50 values below 0.5 mg/ml. The most active samples were onion, oregano and red sweet potato, exhibiting IC50 values below 0.1 mg/ml. When these ten food preparations were retested in RAW 264.7 macrophages, they all inhibited NO production similar to the results obtained in N11 microglia. In addition, English breakfast tea leaves, oyster mushroom, onion, cinnamon and button mushroom preparations suppressed TNF-α production, exhibiting IC50 values below 0.5 mg/ml in RAW 264.7 macrophages. In summary, anti-inflammatory activity in these food samples survived 'cooking'. Provided that individual bioavailability allows active compounds to reach therapeutic levels in target tissues, these foods may be useful in limiting inflammation in a variety of age-related inflammatory diseases. Furthermore, these foods could be a source for the discovery of novel anti-inflammatory drugs.
Dhanushka Gunawardena; Kirubakaran Shanmugam; Mitchell Low; Louise Bennett; Suresh Govindaraghavan; Richard Head; Lezanne Ooi; Gerald Münch. Determination of anti-inflammatory activities of standardised preparations of plant- and mushroom-based foods. European Journal of Nutrition 2013, 53, 335 -343.
AMA StyleDhanushka Gunawardena, Kirubakaran Shanmugam, Mitchell Low, Louise Bennett, Suresh Govindaraghavan, Richard Head, Lezanne Ooi, Gerald Münch. Determination of anti-inflammatory activities of standardised preparations of plant- and mushroom-based foods. European Journal of Nutrition. 2013; 53 (1):335-343.
Chicago/Turabian StyleDhanushka Gunawardena; Kirubakaran Shanmugam; Mitchell Low; Louise Bennett; Suresh Govindaraghavan; Richard Head; Lezanne Ooi; Gerald Münch. 2013. "Determination of anti-inflammatory activities of standardised preparations of plant- and mushroom-based foods." European Journal of Nutrition 53, no. 1: 335-343.