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The combination of natural products with standard chemotherapeutic agents offers a promising strategy to enhance the efficacy or reduce the side effects of standard chemotherapy. Doxorubicin (DOX), a standard drug for breast cancer, has several disadvantages, including severe side effects and the development of drug resistance. Recently, we reported the potential bioactive markers of Australian propolis extract (AP-1) and their broad spectrum of pharmacological activities. In the present study, we explored the synergistic interactions between AP-1 and DOX in the MCF7 breast adenocarcinoma cells using different synergy quantitation models. Biochemometric and metabolomics-driven analysis was performed to identify the potential anticancer metabolites in AP-1. The molecular mechanisms of synergy were studied by analysing the apoptotic profile via flow cytometry, apoptotic proteome array and measuring the oxidative status of the MCF7 cells treated with the most synergistic combination. Furthermore, label-free quantification proteomics analysis was performed to decipher the underlying synergistic mechanisms. Five prenylated stilbenes were identified as the key metabolites in the most active AP-1 fraction. Strong synergy was observed when AP-1 was combined with DOX in the ratio of 100:0.29 (w/w) as validated by different synergy quantitation models implemented. AP-1 significantly enhanced the inhibitory effect of DOX against MCF7 cell proliferation in a dose-dependent manner with significant inhibition of the reactive oxygen species (p< 0.0001) compared to DOX alone. AP-1 enabled the reversal of DOX-mediated necrosis to programmed cell death, which may be advantageous to decline DOX-related side effects. AP-1 also significantly enhanced the apoptotic effect of DOX after 24 h of treatment with significant upregulation of catalase, HTRA2/Omi, FADD together with DR5 and DR4 TRAIL-mediated apoptosis (p< 0.05), contributing to the antiproliferative activity of AP-1. Significant upregulation of pro-apoptotic p27, PON2 and catalase with downregulated anti-apoptotic XIAP, HSP60 and HIF-1α, and increased antioxidant proteins (catalase and PON2) may be associated with the improved apoptosis and oxidative status of the synergistic combination-treated MCF7 cells compared to the mono treatments. Shotgun proteomics identified 21 significantly dysregulated proteins in the synergistic combination-treated cells versus the mono treatments. These proteins were involved in the TP53/ATM-regulated non-homologous end-joining pathway and double-strand breaks repairs, recruiting the overexpressed BRCA1 and suppressed RIF1 encoded proteins. The overexpression of UPF2 was noticed in the synergistic combination treatment, which could assist in overcoming doxorubicin resistance-associated long non-coding RNA and metastasis of the MCF7 cells. In conclusion, we identified the significant synergy and highlighted the key molecular pathways in the interaction between AP-1 and DOX in the MCF7 cells together with the AP-1 anticancer metabolites. Further in vivo and clinical studies are warranted on this synergistic combination.
Muhammad Alsherbiny; Deep Bhuyan; Ibrahim Radwan; Dennis Chang; Chun-Guang Li. Metabolomic Identification of Anticancer Metabolites of Australian Propolis and Proteomic Elucidation of Its Synergistic Mechanisms with Doxorubicin in the MCF7 Cells. International Journal of Molecular Sciences 2021, 22, 7840 .
AMA StyleMuhammad Alsherbiny, Deep Bhuyan, Ibrahim Radwan, Dennis Chang, Chun-Guang Li. Metabolomic Identification of Anticancer Metabolites of Australian Propolis and Proteomic Elucidation of Its Synergistic Mechanisms with Doxorubicin in the MCF7 Cells. International Journal of Molecular Sciences. 2021; 22 (15):7840.
Chicago/Turabian StyleMuhammad Alsherbiny; Deep Bhuyan; Ibrahim Radwan; Dennis Chang; Chun-Guang Li. 2021. "Metabolomic Identification of Anticancer Metabolites of Australian Propolis and Proteomic Elucidation of Its Synergistic Mechanisms with Doxorubicin in the MCF7 Cells." International Journal of Molecular Sciences 22, no. 15: 7840.
The association between human gut microbiota and cancers has been an evolving field of biomedical research in recent years. The gut microbiota is composed of the microorganisms residing in the gastrointestinal system that interact with the host to regulate behaviours and biochemical processes within the gut. This symbiotic physiological interaction between the gut and the microbiota plays a significant role in the modulation of gut homeostasis, in which perturbations to the microbiota, also known as dysbiosis can lead to the onset of diseases, including cancer. In this review, we analysed the current literature to understand the role of gut microbiota in the five most prevalent cancer types, namely colon (colorectal), lung, breast, prostate, and stomach cancers. Recent studies have observed the immunomodulatory and anti-tumoural effects of gut microbiota in cancers. Furthermore, gut microbial dysbiosis can induce the release of toxic metabolites and exhibit pro-tumoural effects in the host. The gut microbiota was observed to have clinical implications in each cancer type in addition to regulating the efficacy of standard chemotherapy and natural anticancer agents. However, further research is warranted to understand the complex role of gut microbiota in the prevention, diagnosis, treatment, and prognoses of cancer.
Kayla Jaye; Chun Guang Li; Deep Jyoti Bhuyan. The complex interplay of gut microbiota with the five most common cancer types: From carcinogenesis to therapeutics to prognoses. Critical Reviews in Oncology/Hematology 2021, 165, 103429 .
AMA StyleKayla Jaye, Chun Guang Li, Deep Jyoti Bhuyan. The complex interplay of gut microbiota with the five most common cancer types: From carcinogenesis to therapeutics to prognoses. Critical Reviews in Oncology/Hematology. 2021; 165 ():103429.
Chicago/Turabian StyleKayla Jaye; Chun Guang Li; Deep Jyoti Bhuyan. 2021. "The complex interplay of gut microbiota with the five most common cancer types: From carcinogenesis to therapeutics to prognoses." Critical Reviews in Oncology/Hematology 165, no. : 103429.
The outbreak of the novel coronavirus, severe acute respiratory syndrome (SARS)–CoV-2, has gained unprecedented global attention. SARS-CoV-2, which causes the newly described coronavirus disease 2019 (COVID-19), has affected millions of people and led to over 1.9 million deaths worldwide by the beginning of January 2021. Several governments have opted for lockdown as one of the measures to combat the rapidly increasing number of COVID-19 cases. Academic institutions (i.e., universities, colleges, research centers and national laboratories), which are home to thousands of students, researchers, technicians, and administrative staff, have strictly followed government regulations. Due to the lockdown, the majority of academics have been facing various challenges, especially in transitioning from classroom to remote teaching and conducting research activities from a home office. This article from an early-career researchers’ perspective addresses the common challenges that academic institutions have encountered and possible strategies they have adopted to mitigate those challenges at the individual organizational level. Furthermore, we propose a framework to facilitate the handling of such crisis in any near future at the organizational level. We hope academics, policymakers and (non) government organizations across the globe will find this perspective a call to better improve the overall infrastructure of academic institutions.
Jagannath Biswakarma; Danielle Rushworth; Gitika Srivastava; Gagandeep Singh; Kyounglim Kang; Subhasish Das; Surendra Babu Anantharaman; Meret Aeppli; Andrea L. Popp; Deep Jyoti Bhuyan. Organizational Level Responses to the COVID-19 Outbreak: Challenges, Strategies and Framework for Academic Institutions. Frontiers in Communication 2021, 6, 1 .
AMA StyleJagannath Biswakarma, Danielle Rushworth, Gitika Srivastava, Gagandeep Singh, Kyounglim Kang, Subhasish Das, Surendra Babu Anantharaman, Meret Aeppli, Andrea L. Popp, Deep Jyoti Bhuyan. Organizational Level Responses to the COVID-19 Outbreak: Challenges, Strategies and Framework for Academic Institutions. Frontiers in Communication. 2021; 6 ():1.
Chicago/Turabian StyleJagannath Biswakarma; Danielle Rushworth; Gitika Srivastava; Gagandeep Singh; Kyounglim Kang; Subhasish Das; Surendra Babu Anantharaman; Meret Aeppli; Andrea L. Popp; Deep Jyoti Bhuyan. 2021. "Organizational Level Responses to the COVID-19 Outbreak: Challenges, Strategies and Framework for Academic Institutions." Frontiers in Communication 6, no. : 1.
The broad-spectrum pharmacological activity of Australian propolis and identification of key markers of propolis samples from Australia, Brazil and China.
Deep Jyoti Bhuyan; Muhammad A. Alsherbiny; Mitchell Nolan Low; Xian Zhou; Kirandeep Kaur; George Li; Chun Guang Li. Broad-spectrum pharmacological activity of Australian propolis and metabolomic-driven identification of marker metabolites of propolis samples from three continents. Food & Function 2021, 1 .
AMA StyleDeep Jyoti Bhuyan, Muhammad A. Alsherbiny, Mitchell Nolan Low, Xian Zhou, Kirandeep Kaur, George Li, Chun Guang Li. Broad-spectrum pharmacological activity of Australian propolis and metabolomic-driven identification of marker metabolites of propolis samples from three continents. Food & Function. 2021; ():1.
Chicago/Turabian StyleDeep Jyoti Bhuyan; Muhammad A. Alsherbiny; Mitchell Nolan Low; Xian Zhou; Kirandeep Kaur; George Li; Chun Guang Li. 2021. "Broad-spectrum pharmacological activity of Australian propolis and metabolomic-driven identification of marker metabolites of propolis samples from three continents." Food & Function , no. : 1.
Neuroinflammation is believed to play a primary role in the pathogenesis of most neurodegenerative diseases including Alzheimer’s disease, Parkinson’s and schizophrenia. Currently, suitable in vitro neuroinflammation models for studying cellular interactions and inflammatory mechanisms at the neurovascular unit are still scarce. In this study, we established an experimentally flexible tri‐culture neuroinflammation model combining murine microglial cells (N11), neurons (N2A) and brain microvascular endothelial MVEC(B3) cells (MVEC) in a transwell co‐culture system stimulated with lipopolysaccharides (LPS). Neuroinflammation was induced in this tri‐culture model as manifested by activated N11 cells via toll‐like receptor 4, resulting in increased release of proinflammatory mediators (nitric oxide, interleukin‐6, and tumor necrosis factor‐α) through the activation of nuclear factor‐κB signaling pathways. The released inflammatory cytokines from N11 in turn, damaged the tight junction in MVEC cells, increased permeability of endothelial barrier, and induced Tau phosphorylation and upregulated caspase‐3 expression in N2A cells, leading to neuroinflammation injury. In summary, this tri‐culture inflammation model mimics the microenvironment, the cellular crosstalk and the molecular events that take place during neuroinflammation. It provides a robust in vitro model for studying neuroinflammation mechanisms and screening for potential therapeutic compounds or drugs candidates to treat various neurodegenerative diseases.
Yan‐Fang Zheng; Xian Zhou; Dennis Chang; Deep Jyoti Bhuyan; Jie Ping Zhang; Wen‐Zhen Yu; Xia‐Sen Jiang; Sai Wang Seto; Seung Yeon Yeon; Jia Li; Chun Guang Li. A novel tri‐culture model for neuroinflammation. Journal of Neurochemistry 2020, 156, 249 -261.
AMA StyleYan‐Fang Zheng, Xian Zhou, Dennis Chang, Deep Jyoti Bhuyan, Jie Ping Zhang, Wen‐Zhen Yu, Xia‐Sen Jiang, Sai Wang Seto, Seung Yeon Yeon, Jia Li, Chun Guang Li. A novel tri‐culture model for neuroinflammation. Journal of Neurochemistry. 2020; 156 (2):249-261.
Chicago/Turabian StyleYan‐Fang Zheng; Xian Zhou; Dennis Chang; Deep Jyoti Bhuyan; Jie Ping Zhang; Wen‐Zhen Yu; Xia‐Sen Jiang; Sai Wang Seto; Seung Yeon Yeon; Jia Li; Chun Guang Li. 2020. "A novel tri‐culture model for neuroinflammation." Journal of Neurochemistry 156, no. 2: 249-261.
Chinese Herbal Medicine (CHM), an integral part of Traditional Chinese Medicine, has been implementing complex herbal formulae usually consisting of two or more medicinal herbs for the prevention and treatment of various diseases since antiquity. The key mechanisms of action of CHM for the systematic management of many diseases largely rely on the concept of synergy to reach an optimal clinical outcome with minimal side effects. With the development of the modern analytical tools and computational programs, a number of methodologies have been applied to evaluate the synergy among herbal ingredients and chemical constituents in CHM. In this chapter, we introduce the concept of synergy in CHM, review studies that investigated synergy of CHM for the management of cancer, diabetes, musculoskeletal pain, cardiovascular, inflammatory, hepatic, infectious and oxidative stress-related diseases. The complex synergistic interactions of CHM components in pharmacokinetics studies and biological networks are also discussed. Despite some of the scientific reports supporting the synergistic effects of multi-herbal and/or herb-drug combinations, the level of evidence remains low particularly in terms of their molecular mechanisms of action and clinical relevance. Significant challenges in the development of suitable methods for CHM synergistic studies are also mentioned.
Deep Jyoti Bhuyan; Saumya Perera; Kirandeep Kaur; Muhammad A. Alsherbiny; Mitchell Low; Sai-Wang Seto; Chun-Guang Li; Xian Zhou. Synergistic Effects of Chinese Herbal Medicine and Biological Networks. A Critical Reflection on Automated Science 2020, 393 -436.
AMA StyleDeep Jyoti Bhuyan, Saumya Perera, Kirandeep Kaur, Muhammad A. Alsherbiny, Mitchell Low, Sai-Wang Seto, Chun-Guang Li, Xian Zhou. Synergistic Effects of Chinese Herbal Medicine and Biological Networks. A Critical Reflection on Automated Science. 2020; ():393-436.
Chicago/Turabian StyleDeep Jyoti Bhuyan; Saumya Perera; Kirandeep Kaur; Muhammad A. Alsherbiny; Mitchell Low; Sai-Wang Seto; Chun-Guang Li; Xian Zhou. 2020. "Synergistic Effects of Chinese Herbal Medicine and Biological Networks." A Critical Reflection on Automated Science , no. : 393-436.
A large amount of mushroom waste is generated during mushroom production (accounting for up to 20% of total production) and is mainly composed of mushrooms that do not meet the specifications set by retailers because of misshapen caps and/or stalks. Mushrooms are notable for their ergosterol (a precursor of vitamin D2) content which is converted to vitamin D2 after exposure to natural or artificial ultraviolet (UV) irradiation. Therefore, mushroom waste could be used as a source for the recovery of both ergosterol and vitamin D2 which could be valorized by both pharmaceutical and food industries. The current review presents a comprehensive summary of research performed regarding the extraction, purification and determination of ergosterol and vitamin D2 (ergocalciferol) from mushroom matrices. Additionally, studies related to the impact of sample preparation and especially of drying methods on the retention of ergosterol and vitamin D2 are presented. Finally, the potential valorization of mushroom waste sterols by food and pharmaceutical industries is discussed. Ergosterol and vitamin D2 contents vary among different mushroom species. Sample drying is a crucial step that precedes sterol extraction and has a significant impact on the retention of ergosterol and vitamin D2. The extraction of sterols from mushrooms can be conducted by either conventional (e.g., Soxhlet extraction) or non-conventional methods (e.g., ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE), deep eutectic solvents (DES) extraction, supercritical fluid extraction (SFE), and pressurized liquid extraction (PLE)) or their combination. The application of non-conventional methods such as UAE and MAE facilitate in shorter extraction times than the conventional methods. The valorization of mushroom extracts enriched in ergosterol and vitamin D2 by both pharmaceutical and food industries requires further work.
Konstantinos Papoutsis; Simona Grasso; Ajay Menon; Nigel P. Brunton; James Lyng; Jean-Christophe Jacquier; Deep Jyoti Bhuyan. Recovery of ergosterol and vitamin D2 from mushroom waste - Potential valorization by food and pharmaceutical industries. Trends in Food Science & Technology 2020, 99, 351 -366.
AMA StyleKonstantinos Papoutsis, Simona Grasso, Ajay Menon, Nigel P. Brunton, James Lyng, Jean-Christophe Jacquier, Deep Jyoti Bhuyan. Recovery of ergosterol and vitamin D2 from mushroom waste - Potential valorization by food and pharmaceutical industries. Trends in Food Science & Technology. 2020; 99 ():351-366.
Chicago/Turabian StyleKonstantinos Papoutsis; Simona Grasso; Ajay Menon; Nigel P. Brunton; James Lyng; Jean-Christophe Jacquier; Deep Jyoti Bhuyan. 2020. "Recovery of ergosterol and vitamin D2 from mushroom waste - Potential valorization by food and pharmaceutical industries." Trends in Food Science & Technology 99, no. : 351-366.
Persea americana, commonly known as avocado, has recently gained substantial popularity and is often marketed as a “superfood” because of its unique nutritional composition, antioxidant content, and biochemical profile. However, the term “superfood” can be vague and misleading, as it is often associated with unrealistic health claims. This review draws a comprehensive summary and assessment of research performed in the last few decades to understand the nutritional and therapeutic properties of avocado and its bioactive compounds. In particular, studies reporting the major metabolites of avocado, their antioxidant as well as bioavailability and pharmacokinetic properties, are summarized and assessed. Furthermore, the potential of avocado in novel drug discovery for the prevention and treatment of cancer, microbial, inflammatory, diabetes, and cardiovascular diseases is highlighted. This review also proposes several interesting future directions for avocado research.
Deep Jyoti Bhuyan; Muhammad A. Alsherbiny; Saumya Perera; Mitchell Low; Amrita Basu; Okram Abemsana Devi; Mridula Saikia Barooah; Chun Guang Li; Konstantinos Papoutsis; Low; Basu; Devi; Li. The Odyssey of Bioactive Compounds in Avocado (Persea americana) and their Health Benefits. Antioxidants 2019, 8, 426 .
AMA StyleDeep Jyoti Bhuyan, Muhammad A. Alsherbiny, Saumya Perera, Mitchell Low, Amrita Basu, Okram Abemsana Devi, Mridula Saikia Barooah, Chun Guang Li, Konstantinos Papoutsis, Low, Basu, Devi, Li. The Odyssey of Bioactive Compounds in Avocado (Persea americana) and their Health Benefits. Antioxidants. 2019; 8 (10):426.
Chicago/Turabian StyleDeep Jyoti Bhuyan; Muhammad A. Alsherbiny; Saumya Perera; Mitchell Low; Amrita Basu; Okram Abemsana Devi; Mridula Saikia Barooah; Chun Guang Li; Konstantinos Papoutsis; Low; Basu; Devi; Li. 2019. "The Odyssey of Bioactive Compounds in Avocado (Persea americana) and their Health Benefits." Antioxidants 8, no. 10: 426.
Pancreatic cancer (PC) is one of the most devastating human cancers, and despite the significant advances in the current therapeutic options, the overall survival rate for PC has remained static for the past 50 years. Plant-derived bioactive compounds play a vital role in cancer therapeutics by providing new lead compounds for future drug development. Therefore, the isolation, characterization, and identification of new bioactive compounds for the prevention and treatment of cancer continue to be an important aspect of natural product research. Many in vitro and in vivo studies published in the last few decades have established strong links between the phytochemical profile of eucalypts and anticancer activity. However, only a small number of these reports have attempted to demonstrate a relationship between the biological activity of eucalypt extracts and PC. This review focuses on potential anti-PC effects of an array of bioactive compounds present in various species of eucalypts. It also highlights the necessity for further in vitro and in vivo studies to develop a complete understanding of the potential this group of plants has for the development of potent and specific chemotherapeutic drugs for PC.
Deep Jyoti Bhuyan; Quan Vuong; Anita C. Chalmers; Michael C. Bowyer; Christopher J. Scarlett. An Array of Bioactive Compounds From Australian Eucalypts and Their Relevance in Pancreatic Cancer Therapeutics. Pancreas 2018, 47, 690 -707.
AMA StyleDeep Jyoti Bhuyan, Quan Vuong, Anita C. Chalmers, Michael C. Bowyer, Christopher J. Scarlett. An Array of Bioactive Compounds From Australian Eucalypts and Their Relevance in Pancreatic Cancer Therapeutics. Pancreas. 2018; 47 (6):690-707.
Chicago/Turabian StyleDeep Jyoti Bhuyan; Quan Vuong; Anita C. Chalmers; Michael C. Bowyer; Christopher J. Scarlett. 2018. "An Array of Bioactive Compounds From Australian Eucalypts and Their Relevance in Pancreatic Cancer Therapeutics." Pancreas 47, no. 6: 690-707.
New therapeutic strategies such as the development of novel drugs and combinatorial therapies with existing chemotherapeutic agents are urgently needed to improve the clinical prognosis of pancreatic cancer. We have previously reported the antiproliferative properties of aqueous crude Eucalyptus microcorys extract against pancreatic cancer cell lines. In this study, bioassay-guided fractionation of the aqueous crude E. microcorys extract using RP-HPLC and subsequent assessment of the resultant fractions (F1–F5) for their antioxidant activity and cytotoxicity against pancreatic cancer cell lines were performed. The molecular mechanisms associated with the cytotoxicity was characterised by studying the effects of the most potent fraction-1 (F1) on apoptosis and cell cycle profiles as well as its phytochemical constituents by LC-ESI/MS/MS. F1 displayed significantly greater antioxidant activity in three different assays (p < 0.05). Moreover, F1 exhibited significantly greater antiproliferative activity (IC50 = 93.11 ± 3.43 μg/mL) against MIA PaCa-2 cells compared to the other four fractions (p < 0.05). F1 induced apoptosis by regulating key apoptotic proteins- Bcl-2, Bak, Bax, cleaved PARP, procaspase-3 and cleaved caspase-3 in MIA PaCa-2 cells, suggesting the involvement of intrinsic mitochondrial apoptotic pathway and arrested cells at G2/M phase. A combination of gemcitabine and F1 exerted a greater effect on apoptosis and cell cycle arrest than F1 or gemcitabine alone (p < 0.05). LC-ESI/MS/MS revealed the tentative identities of phytochemicals present in F1 and their similarities with the phenolic compounds previously reported in Eucalyptus with antipancreatic cancer activity. Our study shows that the polyphenol and antioxidant-rich fraction of E. microcorys extract is a promising candidate for developing mono or combination therapies against pancreatic cancer.
Deep Jyoti Bhuyan; Quan Vuong; Danielle R. Bond; Anita C. Chalmers; Michael C. Bowyer; Christopher J. Scarlett. Eucalyptus microcorys leaf extract derived HPLC-fraction reduces the viability of MIA PaCa-2 cells by inducing apoptosis and arresting cell cycle. Biomedicine & Pharmacotherapy 2018, 105, 449 -460.
AMA StyleDeep Jyoti Bhuyan, Quan Vuong, Danielle R. Bond, Anita C. Chalmers, Michael C. Bowyer, Christopher J. Scarlett. Eucalyptus microcorys leaf extract derived HPLC-fraction reduces the viability of MIA PaCa-2 cells by inducing apoptosis and arresting cell cycle. Biomedicine & Pharmacotherapy. 2018; 105 ():449-460.
Chicago/Turabian StyleDeep Jyoti Bhuyan; Quan Vuong; Danielle R. Bond; Anita C. Chalmers; Michael C. Bowyer; Christopher J. Scarlett. 2018. "Eucalyptus microcorys leaf extract derived HPLC-fraction reduces the viability of MIA PaCa-2 cells by inducing apoptosis and arresting cell cycle." Biomedicine & Pharmacotherapy 105, no. : 449-460.
Australia is home to over 800 different species of Eucalyptus and traditionally, many Eucalyptus species have been utilised to heal wounds and treat fungal infections by the Indigenous people of Australia. In view of this, our study was designed to investigate the phytochemical, antibacterial and antifungal properties of crude aqueous extract of E. microcorys leaves. The freeze-dried powdered extract was prepared and the phytochemical profile was studied by analysing the total phenolic content (TPC), total flavonoid content (TFC), proanthocyanidins, antioxidants and saponins. The TPC, TFC and proanthocyanidin values found were: 501.76 ± 14.47 mg of gallic acid equivalents per g, 61.53 ± 0.83 mg of rutin equivalents per g and 10.76 ± 0.89 mg of catechin equivalents per g, respectively. The antioxidant values expressed in mg trolox equivalents per g of extract (mg TE/g) were: ABTS = 1073.13 ± 10.73 mg TE/g, DPPH = 1035.44 ± 65.54 mg TE/g and CUPRAC = 1524.30 ± 66.43 mg TE/g. The powdered extract was also evaluated for activity against three pathogenic bacterial strains (Escherichia coli, Enterobacter aerogenes, Staphylococcus lugdunensis); and three fungal strains (Geotrichum candidum, Aspergillus brasiliensis and Candida albicans) using the disc diffusion method and 96 well plate-based method with resazurin dye. The extract exhibited clear zones of inhibition against the tested bacteria and fungi. Minimum inhibitory concentration (MIC) values were demonstrated to be: A. brasiliensis = 2.44 μg/mL, G. candidum = 4.88 μg/mL, S. lugdunensis = 78 μg/mL, E. coli = 156.25 μg/mL, E. aerogenes = 312.5 μg/mL and C. albicans = 1250 μg/mL. These results reveal the significant potential of E. microcorys as a source of phenolics, antioxidants and antimicrobial agents and also highlight the necessity of further purification and characterisation of solitary bioactive compounds for their prospective applications in food, nutraceutical and pharmaceutical industries
Deep Jyoti Bhuyan; Quan Vuong; A.C. Chalmers; I.A. van Altena; M.C. Bowyer; C.J. Scarlett. Phytochemical, antibacterial and antifungal properties of an aqueous extract of Eucalyptus microcorys leaves. South African Journal of Botany 2017, 112, 180 -185.
AMA StyleDeep Jyoti Bhuyan, Quan Vuong, A.C. Chalmers, I.A. van Altena, M.C. Bowyer, C.J. Scarlett. Phytochemical, antibacterial and antifungal properties of an aqueous extract of Eucalyptus microcorys leaves. South African Journal of Botany. 2017; 112 ():180-185.
Chicago/Turabian StyleDeep Jyoti Bhuyan; Quan Vuong; A.C. Chalmers; I.A. van Altena; M.C. Bowyer; C.J. Scarlett. 2017. "Phytochemical, antibacterial and antifungal properties of an aqueous extract of Eucalyptus microcorys leaves." South African Journal of Botany 112, no. : 180-185.
In spite of the recent advancements in oncology, the overall survival rate for pancreatic cancer has not improved over the last five decades. Eucalypts have been linked with cytotoxic and anticancer properties in various studies; however, there is very little scientific evidence that supports the direct role of eucalypts in the treatment of pancreatic cancer. This study assessed the anticancer properties of aqueous and ethanolic extracts of four Eucalyptus species using an MTT assay. The most promising extracts were further evaluated using a CCK-8 assay. Apoptotic studies were performed using a caspase 3/7 assay in MIA PaCa-2 cells. The aqueous extract of Eucalyptus microcorys leaf and the ethanolic extract of Eucalyptus microcorys fruit inhibited the growth of glioblastoma, neuroblastoma, lung and pancreatic cancer cells by more than 80% at 100 μg/mL. The E. microcorys and Eucalyptus saligna extracts showed lower GI50 values than the ethanolic Eucalyptus robusta extract in MIA PaCa-2 cells. Aqueous E. microcorys leaf and fruit extracts at 100 μg/mL exerted significantly higher cell growth inhibition in MIA PaCa-2 cells than other extracts (p < 0.05). Statistically similar IC50 values (p > 0.05) were observed in aqueous E. microcorys leaf (86.05 ± 4.75 μg/mL) and fruit (64.66 ± 15.97 μg/mL) and ethanolic E. microcorys leaf (79.30 ± 29.45 μg/mL) extracts in MIA PaCa-2 cells using the CCK-8 assay. Caspase 3/7-mediated apoptosis and morphological changes of cells were also witnessed in MIA PaCa-2 cells after 24 h of treatment with the extracts. This study highlighted the significance of E. microcorys as an important source of phytochemicals with efficacy against pancreatic cancer cells. Further studies are warranted to purify and structurally identify individual compounds and elucidate their mechanisms of action for the development of more potent and specific chemotherapeutic agents for pancreatic cancer.
Deep Jyoti Bhuyan; Jennette Sakoff; Danielle Bond; Melanie Predebon; Quan Vuong; Anita C. Chalmers; Ian A. Van Altena; Michael C. Bowyer; Christopher J. Scarlett. In vitro anticancer properties of selected Eucalyptus species. In Vitro Cellular & Developmental Biology - Animal 2017, 53, 604 -615.
AMA StyleDeep Jyoti Bhuyan, Jennette Sakoff, Danielle Bond, Melanie Predebon, Quan Vuong, Anita C. Chalmers, Ian A. Van Altena, Michael C. Bowyer, Christopher J. Scarlett. In vitro anticancer properties of selected Eucalyptus species. In Vitro Cellular & Developmental Biology - Animal. 2017; 53 (7):604-615.
Chicago/Turabian StyleDeep Jyoti Bhuyan; Jennette Sakoff; Danielle Bond; Melanie Predebon; Quan Vuong; Anita C. Chalmers; Ian A. Van Altena; Michael C. Bowyer; Christopher J. Scarlett. 2017. "In vitro anticancer properties of selected Eucalyptus species." In Vitro Cellular & Developmental Biology - Animal 53, no. 7: 604-615.
While the pharmacological and toxicological properties of eucalypts are well known in indigenous Australian medicinal practice, investigations of the bioactivity of eucalypt extracts against high mortality diseases such as pancreatic cancer in Western medicine have to date been limited, particularly amongst the genera Corymbia and Angophora. Four Angophora and Corymbia species were evaluated for their phytochemical profile and efficacy against both primary and secondary pancreatic cancer cell lines. The aqueous leaf extract of Angophora hispida exhibited statistically higher total phenolic content (107.85 ± 1.46 mg of gallic acid equiv. per g) and total flavonoid content (57.96 ± 1.93 mg rutin equiv. per g) and antioxidant capacity compared to the other tested eucalypts (P < 0.05). Both A. hispida and A. floribunda aqueous extracts showed statistically similar saponin contents. Angophora floribunda extract exerted significantly greater cell growth inhibition of 77.91 ± 4.93% followed by A. hispida with 62.04 ± 7.47% (P < 0.05) at 100 μg/ml in MIA PaCa-2 cells with IC50 values of 75.58 and 87.28 μg/ml, respectively. More studies are required to isolate and identify the bioactive compounds from these two Angophora species and to determine their mode of action against pancreatic malignancies.
Deep Jyoti Bhuyan; Quan V. Vuong; Danielle R. Bond; Anita C. Chalmers; Ian A. van Altena; Michael C. Bowyer; Christopher J. Scarlett. Exploring the Least Studied Australian Eucalypt Genera: Corymbia and Angophora for Phytochemicals with Anticancer Activity against Pancreatic Malignancies. Chemistry & Biodiversity 2017, 14, e1600291 .
AMA StyleDeep Jyoti Bhuyan, Quan V. Vuong, Danielle R. Bond, Anita C. Chalmers, Ian A. van Altena, Michael C. Bowyer, Christopher J. Scarlett. Exploring the Least Studied Australian Eucalypt Genera: Corymbia and Angophora for Phytochemicals with Anticancer Activity against Pancreatic Malignancies. Chemistry & Biodiversity. 2017; 14 (3):e1600291.
Chicago/Turabian StyleDeep Jyoti Bhuyan; Quan V. Vuong; Danielle R. Bond; Anita C. Chalmers; Ian A. van Altena; Michael C. Bowyer; Christopher J. Scarlett. 2017. "Exploring the Least Studied Australian Eucalypt Genera: Corymbia and Angophora for Phytochemicals with Anticancer Activity against Pancreatic Malignancies." Chemistry & Biodiversity 14, no. 3: e1600291.
This study is designed to develop ultrasonic conditions as an advanced technique for optimal recovery of phenolics and antioxidants from Eucalyptus robusta leaf and to evaluate the impact of solvents, temperature, sonication time and power on ultrasound-assisted extraction of these compounds. Temperature has the greatest impact on the total phenolic content (TPC) yield followed by time and power. A yield of 163.68 ± 2.13 mg GAE/g of TPC is observed using 250 W ultrasonic power for 90 min at 60°C with water. This study validates UAE as an efficient, green, and sustainable technique for extracting phenolics from E. robusta.
Deep Jyoti Bhuyan; Quan Vuong; Anita C. Chalmers; Ian A. Van Altena; Michael C. Bowyer; Christopher J. Scarlett. Development of the ultrasonic conditions as an advanced technique for extraction of phenolic compounds from Eucalyptus robusta. Separation Science and Technology 2016, 52, 100 -112.
AMA StyleDeep Jyoti Bhuyan, Quan Vuong, Anita C. Chalmers, Ian A. Van Altena, Michael C. Bowyer, Christopher J. Scarlett. Development of the ultrasonic conditions as an advanced technique for extraction of phenolic compounds from Eucalyptus robusta. Separation Science and Technology. 2016; 52 (1):100-112.
Chicago/Turabian StyleDeep Jyoti Bhuyan; Quan Vuong; Anita C. Chalmers; Ian A. Van Altena; Michael C. Bowyer; Christopher J. Scarlett. 2016. "Development of the ultrasonic conditions as an advanced technique for extraction of phenolic compounds from Eucalyptus robusta." Separation Science and Technology 52, no. 1: 100-112.
The individual and interactive impacts of guar gum and glycerol on the pea starch-based edible film characteristics were examined using three factors with three level Box–Behnken response surface design (BBD). The results showed that density and elongation at break were only significantly (p < 0.05) affected by pea starch and guar gum in a positive linear fashion. The quadratic regression coefficient of pea starch showed a significant effect (p < 0.05) on thickness, density, puncture force, water vapor permeability, and tensile strength. While tensile strength and Young's modulus affected by the quadratic regression coefficient of glycerol and guar gum, respectively. The results were analyzed using Pareto analysis of variance (ANOVA) and the developed predictive equations for each response variable presented reliable and satisfactory fit with high coefficient of determination (R2) values (≥0.96). The optimized conditions with the goal of maximizing mechanical properties and minimizing water vapor permeability were 2.5 g pea starch, 0.3 g guar gum, and 25% w/w glycerol based on the dry film matter in 100 mL of distilled water.
Bahareh Saberi; Rahul Thakur; Deep Jyoti Bhuyan; Quan V. Vuong; Suwimol Chockchaisawasdee; John B. Golding; Christopher J. Scarlett; Costas Stathopoulos. Development of edible blend films with good mechanical and barrier properties from pea starch and guar gum. Starch - Stärke 2016, 69, 1 .
AMA StyleBahareh Saberi, Rahul Thakur, Deep Jyoti Bhuyan, Quan V. Vuong, Suwimol Chockchaisawasdee, John B. Golding, Christopher J. Scarlett, Costas Stathopoulos. Development of edible blend films with good mechanical and barrier properties from pea starch and guar gum. Starch - Stärke. 2016; 69 (1-2):1.
Chicago/Turabian StyleBahareh Saberi; Rahul Thakur; Deep Jyoti Bhuyan; Quan V. Vuong; Suwimol Chockchaisawasdee; John B. Golding; Christopher J. Scarlett; Costas Stathopoulos. 2016. "Development of edible blend films with good mechanical and barrier properties from pea starch and guar gum." Starch - Stärke 69, no. 1-2: 1.
Quan V. Vuong; Anita C. Chalmers; Deep Jyoti Bhuyan; Michael C. Bowyer; Christopher J. Scarlett. ChemInform Abstract: Botanical, Phytochemical, and Anticancer Properties of the Eucalyptus Species. ChemInform 2015, 46, 1 .
AMA StyleQuan V. Vuong, Anita C. Chalmers, Deep Jyoti Bhuyan, Michael C. Bowyer, Christopher J. Scarlett. ChemInform Abstract: Botanical, Phytochemical, and Anticancer Properties of the Eucalyptus Species. ChemInform. 2015; 46 (34):1.
Chicago/Turabian StyleQuan V. Vuong; Anita C. Chalmers; Deep Jyoti Bhuyan; Michael C. Bowyer; Christopher J. Scarlett. 2015. "ChemInform Abstract: Botanical, Phytochemical, and Anticancer Properties of the Eucalyptus Species." ChemInform 46, no. 34: 1.
Eucalyptus robusta (E. robusta) has a significant value in traditional medicine and recently has been shown to possess many pharmacological properties in vitro. This study was designed to utilise microwave-assisted extraction (MAE) to yield optimal total phenolic content (TPC), total flavonoid content (TFC), proanthocyanidin levels and antioxidant capacity from E. robusta using water as the solvent, facilitated by the use of response surface methodology (RSM). A three-level-three-factor Box–Behnken design was implemented to elucidate the effect of irradiation time, power and sample-to-solvent ratio on the yields of these phytochemicals. The results highlighted the accuracy and reliability of RSM as a tool for predicting the yields of TPC, TFC, proanthocyanidins and total antioxidants using MAE. Sample-to-solvent ratio had the greatest impact on the TPC yield followed by power and irradiation time. The optimal MAE conditions for TPC and TFC were 3 min, 600 W power and 2 g/100 mL sample-to-solvent ratio. The experimental yield of TPC was 58.40 ± 1.03 mg GAE/g, and 19.15 ± 1.06 mg RE/g of TFC was obtained under these optimal conditions. These conditions, optimised for maximum TPC yield also liberated 62%, 64.6%, 66.3% and 67% of the maximum proanthocyanidins, ABTS, DPPH and CUPRAC values, respectively. This study revealed that MAE is a reliable and efficient method for extracting high yields of phytochemicals from E. robusta, with significant potential to be up-scaled for industrial, nutraceutical or pharmaceutical applications
Deep Jyoti Bhuyan; Quan Vuong; Anita C. Chalmers; Ian A. van Altena; Michael C. Bowyer; Christopher J. Scarlett. Microwave-assisted extraction of Eucalyptus robusta leaf for the optimal yield of total phenolic compounds. Industrial Crops and Products 2015, 69, 290 -299.
AMA StyleDeep Jyoti Bhuyan, Quan Vuong, Anita C. Chalmers, Ian A. van Altena, Michael C. Bowyer, Christopher J. Scarlett. Microwave-assisted extraction of Eucalyptus robusta leaf for the optimal yield of total phenolic compounds. Industrial Crops and Products. 2015; 69 ():290-299.
Chicago/Turabian StyleDeep Jyoti Bhuyan; Quan Vuong; Anita C. Chalmers; Ian A. van Altena; Michael C. Bowyer; Christopher J. Scarlett. 2015. "Microwave-assisted extraction of Eucalyptus robusta leaf for the optimal yield of total phenolic compounds." Industrial Crops and Products 69, no. : 290-299.
The genus Eucalyptus (Myrtaceae) is mainly native to Australia; however, some species are now distributed globally. Eucalyptus has been used in indigenous Australian medicines for the treatment of a range of aliments including colds, flu, fever, muscular aches, sores, internal pains, and inflammation. Eucalyptus oils containing volatile compounds have been widely used in the pharmaceutical and cosmetics industries for a multitude of purposes. In addition, Eucalyptus extracts containing nonvolatile compounds are also an important source of key bioactive compounds, and several studies have linked Eucalyptus extracts with anticancer properties. With the increasing research interest in Eucalyptus and its health properties, this review briefly outlines the botanical features of Eucalyptus, discusses its traditional use as medicine, and comprehensively reviews its phytochemical and anticancer properties and, finally, proposes trends for future studies.
Quan Vuong; Anita C. Chalmers; Deep Jyoti Bhuyan; Michael C. Bowyer; Christopher J. Scarlett. Botanical, Phytochemical, and Anticancer Properties of theEucalyptusSpecies. Chemistry & Biodiversity 2015, 12, 907 -924.
AMA StyleQuan Vuong, Anita C. Chalmers, Deep Jyoti Bhuyan, Michael C. Bowyer, Christopher J. Scarlett. Botanical, Phytochemical, and Anticancer Properties of theEucalyptusSpecies. Chemistry & Biodiversity. 2015; 12 (6):907-924.
Chicago/Turabian StyleQuan Vuong; Anita C. Chalmers; Deep Jyoti Bhuyan; Michael C. Bowyer; Christopher J. Scarlett. 2015. "Botanical, Phytochemical, and Anticancer Properties of theEucalyptusSpecies." Chemistry & Biodiversity 12, no. 6: 907-924.
Eucalyptus species have found their place in traditional medicine and pharmacological research and they have also been shown to possess a large number of phenolic compounds and antioxidants. The present study sought to implement conventional extraction to yield maximal total phenolic content (TPC), total flavonoid content (TFC), proanthocyanidins, antioxidants, and saponins from E. robusta using different solvents. The most suitable extraction solvent was further employed for extracting phytochemicals from E. saligna, E. microcorys, and E. globulus to select the Eucalyptus species with the greatest bioactive compound content. The results emphasised the efficiency of water in extracting TPC ((150.60 ± 2.47) mg of gallic acid equivalents per g), TFC ((38.83 ± 0.23) mg of rutin equivalents per g), proanthocyanidins ((5.14 ± 0.77) mg of catechin equivalents per g), and antioxidants ABTS ((525.67 ± 1.99) mg of trolox equivalents (TE) per g), DPPH ((378.61 ± 4.72) mg of TE per g); CUPRAC ((607.43 ± 6.69) mg of TE per g) from E. robusta. Moreover, the aqueous extract of E. robusta had the highest TPC, TFC and antioxidant values among the other Eucalyptus species tested. These findings highlighted the efficiency of conventional extraction in extracting natural bioactive compounds from Eucalyptus species for pharmaceutical and nutraceutical applications.
Deep Jyoti Bhuyan; Quan V. Vuong; Anita C. Chalmers; Ian A. Van Altena; Michael C. Bowyer; Christopher J. Scarlett. Investigation of phytochemicals and antioxidant capacity of selected Eucalyptus species using conventional extraction. Chemical Papers 2015, 1 .
AMA StyleDeep Jyoti Bhuyan, Quan V. Vuong, Anita C. Chalmers, Ian A. Van Altena, Michael C. Bowyer, Christopher J. Scarlett. Investigation of phytochemicals and antioxidant capacity of selected Eucalyptus species using conventional extraction. Chemical Papers. 2015; ():1.
Chicago/Turabian StyleDeep Jyoti Bhuyan; Quan V. Vuong; Anita C. Chalmers; Ian A. Van Altena; Michael C. Bowyer; Christopher J. Scarlett. 2015. "Investigation of phytochemicals and antioxidant capacity of selected Eucalyptus species using conventional extraction." Chemical Papers , no. : 1.
Euphol identified in Euphorbia tirucalli (E. tirucalli) has been linked with various health benefits. This study aimed to optimize ultrasonic extraction conditions for euphol from E. tirucalli leaf. Different solvents were tested to determine the most effective solvent for extraction of euphol. Then, response surface methodology (RSM) was employed to optimize ultrasound-assisted extraction conditions including temperature, time and power for maximal extraction of euphol. Our results showed that ethyl acetate:ethanol (4:1, v/v) was the most effective solvent for the extraction of euphol. Ultrasonic temperature and time had a positive impact, whereas, ultrasonic power had a negative effect on the extraction efficiency of euphol. The optimum ultrasonic extraction conditions for euphol were identified as: solvent-to-fresh sample ratio of 100:32 mL/g; ultrasonic temperature of 60 °C; ultrasonic time of 75 min and ultrasonic power of 60% (150 W). Under these optimum conditions, approximately 4.06 mg of euphol could be obtained from one gram of fresh E. tirucalli leaf. This extract also contained phenolic compounds (2.5 mg GAE/g FW) and possessed potent antioxidant capacity. These optimal conditions are applicable for a larger scale to extract and isolate euphol for potential utilization in the pharmaceutical industry. Keyword
Quan Vuong; Van Tang Nguyen; Dang Trung Thanh; Deep Jyoti Bhuyan; Chloe Goldsmith; Elham Sadeqzadeh; Christopher J. Scarlett; Michael C. Bowyer. Optimization of ultrasound-assisted extraction conditions for euphol from the medicinal plant, Euphorbia tirucalli, using response surface methodology. Industrial Crops and Products 2015, 63, 197 -202.
AMA StyleQuan Vuong, Van Tang Nguyen, Dang Trung Thanh, Deep Jyoti Bhuyan, Chloe Goldsmith, Elham Sadeqzadeh, Christopher J. Scarlett, Michael C. Bowyer. Optimization of ultrasound-assisted extraction conditions for euphol from the medicinal plant, Euphorbia tirucalli, using response surface methodology. Industrial Crops and Products. 2015; 63 ():197-202.
Chicago/Turabian StyleQuan Vuong; Van Tang Nguyen; Dang Trung Thanh; Deep Jyoti Bhuyan; Chloe Goldsmith; Elham Sadeqzadeh; Christopher J. Scarlett; Michael C. Bowyer. 2015. "Optimization of ultrasound-assisted extraction conditions for euphol from the medicinal plant, Euphorbia tirucalli, using response surface methodology." Industrial Crops and Products 63, no. : 197-202.