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Alena Gajdošová
Plant Science and Biodiversity Center, Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, 950 07 Nitra, Slovakia

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Communication
Published: 06 June 2021 in Plants
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The aim of this work was to assess the regeneration capacity of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica cv. ‘Jugana’ from different types of explants under various hormonal treatments. The whole leaves, petioles, and internodal segments of in vitro plants were examined as explants. Several plant growth regulators (cytokinins and auxins) were evaluated for their ability to induce adventitious regeneration. Direct and indirect organogenesis was achieved under certain culture conditions in both species. The frequency of shoot regeneration was strongly dependent on concentrations of plant growth regulators in the induction media (L.kamtschatica ‘Jugana’) or concentrations of plant growth regulators in the induction media and type of explant (A. alnifolia var. cusickii). Results showed that leaves were not suitable explants for A. alnifolia var. cusickii. Both species were able to regenerate shoots from internodal segments and petioles. The highest induction of shoots was obtained on Murashige and Skoog (MS) medium enriched with 2 mg/L thidiazuron (TDZ) and 0.5 mg/L indole-3-butyric acid (IBA) for Amelanchier alnifolia and with 1 mg/L TDZ and 0.2 mg/L indole-3-acetic acid (IAA) for L.kamtschatica ‘Jugana’. Obtained adventitious shoots were further proliferated in order to investigate their multiplication capacity. The multiplication of shoots was successful in all cultivars, with the best results reported in A. alnifolia var. cusickii (7.07 shoots/explant on average).

ACS Style

Júlia Hunková; Monika Szabóová; Alena Gajdošová. Protocols for Adventitious Regeneration of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica ‘Jugana’. Plants 2021, 10, 1155 .

AMA Style

Júlia Hunková, Monika Szabóová, Alena Gajdošová. Protocols for Adventitious Regeneration of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica ‘Jugana’. Plants. 2021; 10 (6):1155.

Chicago/Turabian Style

Júlia Hunková; Monika Szabóová; Alena Gajdošová. 2021. "Protocols for Adventitious Regeneration of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica ‘Jugana’." Plants 10, no. 6: 1155.

Journal article
Published: 24 July 2020 in Plants
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Berry fruit species are, in many countries, considered biologically and economically valuable and important species of small fruits. The aim of this work was to examine the influence of either decreased or increased mesos concentrations (MgSO4, CaCl2, and KH2PO4) on shoot multiplication of five cultivars of three small fruit species (Amelanchier alnifolia var. cusickii, Rubus fruticosus ‘Black Satin’ and ‘Loch Ness’, and Vaccinium corymbosum ‘Brigitta Blue’ and ‘Toro’). Mesos nutrients were manipulated from half to four times their base concentration. The results indicate that mesos manipulation significantly influences the number and length of shoots in most of the studied cultivars. The greatest multiplication rate for A. alnifolia was achieved with tripled mesos, whereas ‘Black Satin’ and ‘Loch Ness’ reacted positively to a lower (1–2x) concentration of mesos. Decreasing the concentration of mesos to half led to worse quality in both blackberry and Saskatoon shoots. ‘Brigitta Blue’ was more sensitive to greater mesos concentrations compared to ‘Toro’. Optimizing the mineral nutrition of plants cultivated in vitro enhances their multiplication rate and contributes to a higher production of good quality plantlets.

ACS Style

Júlia Hunková; Alena Gajdošová; Monika Szabóová. Effect of Mesos Components (MgSO4, CaCl2, KH2PO4) on In Vitro Shoot Growth of Blackberry, Blueberry, and Saskatoon. Plants 2020, 9, 935 .

AMA Style

Júlia Hunková, Alena Gajdošová, Monika Szabóová. Effect of Mesos Components (MgSO4, CaCl2, KH2PO4) on In Vitro Shoot Growth of Blackberry, Blueberry, and Saskatoon. Plants. 2020; 9 (8):935.

Chicago/Turabian Style

Júlia Hunková; Alena Gajdošová; Monika Szabóová. 2020. "Effect of Mesos Components (MgSO4, CaCl2, KH2PO4) on In Vitro Shoot Growth of Blackberry, Blueberry, and Saskatoon." Plants 9, no. 8: 935.

Journal article
Published: 28 August 2019 in Journal of Berry Research
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ACS Style

Júlia Hunková; Alena Gajdošová. In vitro rooting and acclimatization of Amelanchier alnifolia (Nutt.) Nutt. ex M. Roem: Testing of auxin, spermidine, and gibberellin for overcoming dormancy. Journal of Berry Research 2019, 9, 549 -561.

AMA Style

Júlia Hunková, Alena Gajdošová. In vitro rooting and acclimatization of Amelanchier alnifolia (Nutt.) Nutt. ex M. Roem: Testing of auxin, spermidine, and gibberellin for overcoming dormancy. Journal of Berry Research. 2019; 9 (3):549-561.

Chicago/Turabian Style

Júlia Hunková; Alena Gajdošová. 2019. "In vitro rooting and acclimatization of Amelanchier alnifolia (Nutt.) Nutt. ex M. Roem: Testing of auxin, spermidine, and gibberellin for overcoming dormancy." Journal of Berry Research 9, no. 3: 549-561.

Journal article
Published: 01 January 2018 in Genetika
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The aim of this work was to evaluate shoot multiplication of two blackberry (Rubus fruticosus L.) cultivars ?Black Satin? and ?Loch Ness? on two culture media: Murashige & Skoog (MS) and its modification Murashige & Skoog Van der Salm medium (MS VDS) which differ only in iron source (FeNaEDTA, FeEDDHA, respectively). Statistical analyses showed no significant difference in shoot multiplication between different iron sources for both tested cultivars. For ?Black Satin? it was shown that double concentration of chelates FeNaEDTA and FeEDDHA in culture media negatively affected on shoot growth and multiplication. These results can be very useful for further optimization of micropropagation process for different Rubus cultivars.

ACS Style

Júlia Hunková; Gabriela Libiaková; Jozef Fejér; Tatjana Vujovic; Alena Gajdošová. Testing of different iron sources and concentrations on shoot multiplication of blackberry (Rubus fruticosus L.). Genetika 2018, 50, 351 -356.

AMA Style

Júlia Hunková, Gabriela Libiaková, Jozef Fejér, Tatjana Vujovic, Alena Gajdošová. Testing of different iron sources and concentrations on shoot multiplication of blackberry (Rubus fruticosus L.). Genetika. 2018; 50 (1):351-356.

Chicago/Turabian Style

Júlia Hunková; Gabriela Libiaková; Jozef Fejér; Tatjana Vujovic; Alena Gajdošová. 2018. "Testing of different iron sources and concentrations on shoot multiplication of blackberry (Rubus fruticosus L.)." Genetika 50, no. 1: 351-356.

Journal article
Published: 01 January 2016 in TURKISH JOURNAL OF AGRICULTURE AND FORESTRY
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ACS Style

Andrea Hricová; Jozef Fejér; Gabriela Libiaková; Monika Szabová; Ján Gažo; Alena Gajdošová. Characterization of phenotypic and nutritional properties ofvaluable Amaranthus cruentus L. mutants. TURKISH JOURNAL OF AGRICULTURE AND FORESTRY 2016, 40, 761 -771.

AMA Style

Andrea Hricová, Jozef Fejér, Gabriela Libiaková, Monika Szabová, Ján Gažo, Alena Gajdošová. Characterization of phenotypic and nutritional properties ofvaluable Amaranthus cruentus L. mutants. TURKISH JOURNAL OF AGRICULTURE AND FORESTRY. 2016; 40 ():761-771.

Chicago/Turabian Style

Andrea Hricová; Jozef Fejér; Gabriela Libiaková; Monika Szabová; Ján Gažo; Alena Gajdošová. 2016. "Characterization of phenotypic and nutritional properties ofvaluable Amaranthus cruentus L. mutants." TURKISH JOURNAL OF AGRICULTURE AND FORESTRY 40, no. : 761-771.

Journal article
Published: 01 January 2016 in Journal of Central European Agriculture
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The aim of this work was to develop protocols for micropropagation of selected Rubus cultivars – ‘Tulameen’ and ‘Black Jewel’ (Rubus idaeus L.), ‘Bl...

ACS Style

Júlia Hunková; Gabriela Libiaková; Alena Gajdošová. Shoot proliferation ability of selected cultivars of Rubus spp. as influenced by genotype and cytokinin concentration. Journal of Central European Agriculture 2016, 17, 379 -390.

AMA Style

Júlia Hunková, Gabriela Libiaková, Alena Gajdošová. Shoot proliferation ability of selected cultivars of Rubus spp. as influenced by genotype and cytokinin concentration. Journal of Central European Agriculture. 2016; 17 (2):379-390.

Chicago/Turabian Style

Júlia Hunková; Gabriela Libiaková; Alena Gajdošová. 2016. "Shoot proliferation ability of selected cultivars of Rubus spp. as influenced by genotype and cytokinin concentration." Journal of Central European Agriculture 17, no. 2: 379-390.

Journal article
Published: 01 January 2015 in Genetika
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The introduction of foreign DNA into the plant genome by Agrobacterium tumefaciens is a promising technique of targeted gene transfer which depends on good working regeneration system. The aim of the work was to elaborate the system for efficient adventitious organogenesis and transgenic plant regeneration in Rubus fruticosus L. using explants from mature plants. Regeneration of putative transgenic shoots took place from flag explants cultivated vertically on MS medium with 1 mg l-1 TDZ and 0.02 mg l-1 IBA followed by transfer on MS medium with 1 mg l-1 BAP, 0.02 mg l-1 IBA and 0.1 mg l-1 GA3 supplemented with 10-15 mg l-1 hygromycin after transformation by A. tumefaciens strain LBA 4404 carrying plasmid pCambia 1304. Four putative transgenic plants of cv. 'Cacanska Bestrna' were rooted and acclimatized.

ACS Style

Alena Gajdošová; Tatjana Vujovic; Miroslava Súkeníková; Gabriela Libiaková. Improvement of adventitious organogenesis for regeneration of transgenic plants in blackberry. Genetika 2015, 47, 599 -608.

AMA Style

Alena Gajdošová, Tatjana Vujovic, Miroslava Súkeníková, Gabriela Libiaková. Improvement of adventitious organogenesis for regeneration of transgenic plants in blackberry. Genetika. 2015; 47 (2):599-608.

Chicago/Turabian Style

Alena Gajdošová; Tatjana Vujovic; Miroslava Súkeníková; Gabriela Libiaková. 2015. "Improvement of adventitious organogenesis for regeneration of transgenic plants in blackberry." Genetika 47, no. 2: 599-608.

Journal article
Published: 05 October 2014 in Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
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ACS Style

Marcel Raček; Helena Lichtnerová; Marta Dragúňová; Alena Gajdošová; Anna Jakábová. Multiplication of Acer davidii ssp. grosseri (Pax) De Jong under in vitro conditions. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 2014, 58, 147 -152.

AMA Style

Marcel Raček, Helena Lichtnerová, Marta Dragúňová, Alena Gajdošová, Anna Jakábová. Multiplication of Acer davidii ssp. grosseri (Pax) De Jong under in vitro conditions. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis. 2014; 58 (1):147-152.

Chicago/Turabian Style

Marcel Raček; Helena Lichtnerová; Marta Dragúňová; Alena Gajdošová; Anna Jakábová. 2014. "Multiplication of Acer davidii ssp. grosseri (Pax) De Jong under in vitro conditions." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 58, no. 1: 147-152.

Research note
Published: 17 July 2014 in Plant Cell, Tissue and Organ Culture (PCTOC)
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The aim of work was to elaborate the system for Agrobacterium tumefaciens-mediated transformation and transgenic plant regeneration in Rubus fruticosus L. using explants from mature plants. Regeneration of transgenic shoots was achieved from cut ends of petioles completely immersed in the medium using flag explants cultivated vertically on MS medium with 1 mg l−1 TDZ and 0.02 mg l−1 IBA followed by transfer on medium with 1 mg l−1 BAP, 0.02 mg l−1 IBA and 0.1 mg l−1 GA3, both supplemented with 10 and 15 mg l−1 hygromycin after transformation by A. tumefaciens strain LBA 4404/pCambia 1304. Four putative transgenic plants of cv. ‘Čačanska Bestrna’ were rooted, acclimatized and analysed. Transgenic character of the tissue from analysed plants was confirmed by nested PCR and subsequent sequencing.

ACS Style

Miroslava Súkeníková; Gabriela Libiaková; Jana Moravčíková; Andrea Hricová; Alena Gajdosova. Agrobacterium tumefaciens-mediated transformation of blackberry (Rubus fruticosus L.). Plant Cell, Tissue and Organ Culture (PCTOC) 2014, 120, 351 -354.

AMA Style

Miroslava Súkeníková, Gabriela Libiaková, Jana Moravčíková, Andrea Hricová, Alena Gajdosova. Agrobacterium tumefaciens-mediated transformation of blackberry (Rubus fruticosus L.). Plant Cell, Tissue and Organ Culture (PCTOC). 2014; 120 (1):351-354.

Chicago/Turabian Style

Miroslava Súkeníková; Gabriela Libiaková; Jana Moravčíková; Andrea Hricová; Alena Gajdosova. 2014. "Agrobacterium tumefaciens-mediated transformation of blackberry (Rubus fruticosus L.)." Plant Cell, Tissue and Organ Culture (PCTOC) 120, no. 1: 351-354.

Journal article
Published: 01 January 2012 in Acta Horticulturae
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ACS Style

D. Ruzic; T. Vujovic; R. Cerović; M.G. Ostrolucka; A. Gajdošová. MICROPROPAGATION IN VITRO OF HIGHBUSH BLUEBERRY (VACCINIUM CORYMBOSUM L.). Acta Horticulturae 2012, 265 -272.

AMA Style

D. Ruzic, T. Vujovic, R. Cerović, M.G. Ostrolucka, A. Gajdošová. MICROPROPAGATION IN VITRO OF HIGHBUSH BLUEBERRY (VACCINIUM CORYMBOSUM L.). Acta Horticulturae. 2012; (926):265-272.

Chicago/Turabian Style

D. Ruzic; T. Vujovic; R. Cerović; M.G. Ostrolucka; A. Gajdošová. 2012. "MICROPROPAGATION IN VITRO OF HIGHBUSH BLUEBERRY (VACCINIUM CORYMBOSUM L.)." Acta Horticulturae , no. 926: 265-272.

Journal article
Published: 01 January 2012 in Journal of Berry Research
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ACS Style

Djurdjina Ružić; Tatjana Vujović; Gabriela Libiakova; Radosav Cerović; Alena Gajdošova. Micropropagation in vitro of highbush blueberry (Vaccinium corymbosum L.). Journal of Berry Research 2012, 2, 97 -103.

AMA Style

Djurdjina Ružić, Tatjana Vujović, Gabriela Libiakova, Radosav Cerović, Alena Gajdošova. Micropropagation in vitro of highbush blueberry (Vaccinium corymbosum L.). Journal of Berry Research. 2012; 2 (2):97-103.

Chicago/Turabian Style

Djurdjina Ružić; Tatjana Vujović; Gabriela Libiakova; Radosav Cerović; Alena Gajdošova. 2012. "Micropropagation in vitro of highbush blueberry (Vaccinium corymbosum L.)." Journal of Berry Research 2, no. 2: 97-103.

Journal article
Published: 01 May 2011 in Agriculture (Pol'nohospodárstvo)
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Methodological Approaches to Simple Enzyme Polymorphism Analyses of Amaranth Species (AmaranthusSP.)Amaranths have drawn a great interest in last years as an agronomic crop, alternative energy source and ornamental plants around the world. Several of them are cultivated as the leaf vegetables, cereals, or as the colourful, quick growing garden plants. Increasing demand for the breeding and proteomic study inAmaranthusspecies led us to test some methodological approaches to enzyme polymorphism analysis. For these experiments genotypes ofAmaranthus cruentusL. (genotype Ficha) and mutant line of hybrid K-433 were selected, characterized by a good seed quality and quantity, suitable for food production. The attention was devoted to test of published methodology, which were slightly modified for enzyme (ACP, ADH, CAT, DIA, GOT, IDH, MDH, PGD, PGI and PGM) multiplicity analysis for amaranths and testing of feasibility of chosen analysed organ weights, dimensions of Whatman No. 2 wicks and different volumes of extract buffer. Results from enzyme multiplicity analysis are presented by means of photographed fingerprints and phenotypes are expressed in diagrams where positions of zymograme bands are marked by using factors of relative mobility (Rm).

ACS Style

Pavol Múdry; Andrea Hricová; Gabriela Libiaková; Alena Gajdošová. Methodological Approaches to Simple Enzyme Polymorphism Analyses of Amaranth Species (Amaranthus SP.). Agriculture (Pol'nohospodárstvo) 2011, 57, 1 -11.

AMA Style

Pavol Múdry, Andrea Hricová, Gabriela Libiaková, Alena Gajdošová. Methodological Approaches to Simple Enzyme Polymorphism Analyses of Amaranth Species (Amaranthus SP.). Agriculture (Pol'nohospodárstvo). 2011; 57 (1):1-11.

Chicago/Turabian Style

Pavol Múdry; Andrea Hricová; Gabriela Libiaková; Alena Gajdošová. 2011. "Methodological Approaches to Simple Enzyme Polymorphism Analyses of Amaranth Species (Amaranthus SP.)." Agriculture (Pol'nohospodárstvo) 57, no. 1: 1-11.

Journal article
Published: 01 December 2010 in Acta Horticulturae
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ACS Style

I. Iliev; A. Scaltsoyiannes; M. Tsaktsira; A. Gajdošová. MICROPROPAGATION OF BETULA PENDULA ROTH CULTIVARS BY ADVENTITIOUS SHOOT INDUCTION FROM LEAF CALLUS. Acta Horticulturae 2010, 161 -173.

AMA Style

I. Iliev, A. Scaltsoyiannes, M. Tsaktsira, A. Gajdošová. MICROPROPAGATION OF BETULA PENDULA ROTH CULTIVARS BY ADVENTITIOUS SHOOT INDUCTION FROM LEAF CALLUS. Acta Horticulturae. 2010; (885):161-173.

Chicago/Turabian Style

I. Iliev; A. Scaltsoyiannes; M. Tsaktsira; A. Gajdošová. 2010. "MICROPROPAGATION OF BETULA PENDULA ROTH CULTIVARS BY ADVENTITIOUS SHOOT INDUCTION FROM LEAF CALLUS." Acta Horticulturae , no. 885: 161-173.

Book chapter
Published: 17 March 2010 in Plant Cell Culture
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This chapter contains sections titled: Introduction Methods and approaches Troubleshooting References

ACS Style

Ivan Iliev; Alena Gajdošová; Gabriela Libiaková; Shri Mohan Jain. Plant Micropropagation. Plant Cell Culture 2010, 1 -23.

AMA Style

Ivan Iliev, Alena Gajdošová, Gabriela Libiaková, Shri Mohan Jain. Plant Micropropagation. Plant Cell Culture. 2010; ():1-23.

Chicago/Turabian Style

Ivan Iliev; Alena Gajdošová; Gabriela Libiaková; Shri Mohan Jain. 2010. "Plant Micropropagation." Plant Cell Culture , no. : 1-23.

Journal article
Published: 01 January 2010 in Acta Biologica Cracoviensia s. Botanica
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ACS Style

Mária Ostrolucká; Alena Gajdošová; Emília Ondrušková; Miroslava Latečková; Gabriela Libiaková. Effect of Medium pH on Axillary Shoot Proliferation of Selected Vaccinium vitis-idaea L. Cultivars. Acta Biologica Cracoviensia s. Botanica 2010, 52, 1 .

AMA Style

Mária Ostrolucká, Alena Gajdošová, Emília Ondrušková, Miroslava Latečková, Gabriela Libiaková. Effect of Medium pH on Axillary Shoot Proliferation of Selected Vaccinium vitis-idaea L. Cultivars. Acta Biologica Cracoviensia s. Botanica. 2010; 52 (2):1.

Chicago/Turabian Style

Mária Ostrolucká; Alena Gajdošová; Emília Ondrušková; Miroslava Latečková; Gabriela Libiaková. 2010. "Effect of Medium pH on Axillary Shoot Proliferation of Selected Vaccinium vitis-idaea L. Cultivars." Acta Biologica Cracoviensia s. Botanica 52, no. 2: 1.

Journal article
Published: 06 August 2008 in Plant Cell, Tissue and Organ Culture (PCTOC)
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The idea to organize an international meeting on plant biotechnology entitled “Recent Advances in Plant Biotechnology” was born at the Institute of Plant Genetics and Biotechnology of the Slovak Academy of Sciences in Nitra. The first symposium was held in 1995 in Nitra. At this symposium, the participants decided to organize a plant-biotechnology symposium every second year alternating in the Slovak and Czech Republic. The principal aim of these meetings is to bring together researchers from around the world to present current results obtained in the field of plant biotechnology and to induce a fruitful discussion on topics of mutual interest.

ACS Style

Alena Gajdosova. Plant biotechnology: Impact on high quality plant production. Plant Cell, Tissue and Organ Culture (PCTOC) 2008, 94, 233 -233.

AMA Style

Alena Gajdosova. Plant biotechnology: Impact on high quality plant production. Plant Cell, Tissue and Organ Culture (PCTOC). 2008; 94 (3):233-233.

Chicago/Turabian Style

Alena Gajdosova. 2008. "Plant biotechnology: Impact on high quality plant production." Plant Cell, Tissue and Organ Culture (PCTOC) 94, no. 3: 233-233.

Book chapter
Published: 01 January 2007 in Protocols for Micropropagation of Woody Trees and Fruits
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The problems which are connected with traditional propagation methods, e.g. rapid spread of diseases, low propagation coefficient, can be eliminated by using in vitro techniques. In fruit species these techniques assume high practical importance because they enable not only rapid and effective plant propagation, but also elimination of pathogens and this way production of healthy planting material. Disadvantages of in vitro propagation in Vaccinium vitis-idaea L. is higher cost of in vitro plants in comparison with classically propagated plants, mainly in cultivars with natural rich shooting (‘Koralle’, ‘Linnea’). However, this technique could be effectively used for a production of cultivars characteristic by weak shooting (‘Ida’, ‘Sanna’) (Gustavsson & Trajkovski, 1999 Ondrušková et al., 2006). The present paper is focused on description of efficient in vitro regeneration and propagation protocol for Vaccinium vitis-idaea L.

ACS Style

M. G. Ostrolucká; A. Gajdošová; G. Libiaková; E. Ondrušková. Protocol for Micropropagation of Vaccinium Vitis-Idaea L. Protocols for Micropropagation of Woody Trees and Fruits 2007, 457 -464.

AMA Style

M. G. Ostrolucká, A. Gajdošová, G. Libiaková, E. Ondrušková. Protocol for Micropropagation of Vaccinium Vitis-Idaea L. Protocols for Micropropagation of Woody Trees and Fruits. 2007; ():457-464.

Chicago/Turabian Style

M. G. Ostrolucká; A. Gajdošová; G. Libiaková; E. Ondrušková. 2007. "Protocol for Micropropagation of Vaccinium Vitis-Idaea L." Protocols for Micropropagation of Woody Trees and Fruits , no. : 457-464.

Book chapter
Published: 01 January 2007 in Protocols for Micropropagation of Woody Trees and Fruits
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Oaks are important broad-leaved hardwood species of forest ecosystems, valuable from economical and ecological point of view. The preferred means of oak propagation is from seeds, what favours broadening of genetic diversity of these species. However, the possibilities of generative reproduction are limited by long life span necessary for achievement of physiological maturity, by irregular occurrence of mast years, by low crop and quality of seeds and difficulties in acorn storage. Seeds of Quercus species show different degrees of recalcitrance and can be stored only for a short period because of their sensitivity to desiccation. In addition, the conventional method of vegetative propagation of mature trees by cuttings is associated with difficulties in rooting.

ACS Style

M. G. Ostrolucká; A. Gajdošová; G. Libiaková. Protocol for Micropropagation of Quercus spp. Protocols for Micropropagation of Woody Trees and Fruits 2007, 85 -91.

AMA Style

M. G. Ostrolucká, A. Gajdošová, G. Libiaková. Protocol for Micropropagation of Quercus spp. Protocols for Micropropagation of Woody Trees and Fruits. 2007; ():85-91.

Chicago/Turabian Style

M. G. Ostrolucká; A. Gajdošová; G. Libiaková. 2007. "Protocol for Micropropagation of Quercus spp." Protocols for Micropropagation of Woody Trees and Fruits , no. : 85-91.

Book chapter
Published: 01 January 2007 in Protocols for Micropropagation of Woody Trees and Fruits
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The highbush blueberry (Vaccinium corymbosum L.) belongs to the genus Vaccinium L. (Ericaceae) and is widely cultivated in various parts of the world. Most of the high bush blueberry cultivars (V. corymbosum) grow in the midwestern, eastern and central United States and are also cultivated in Europe, Australia and New Zealand. They are commercially and biologically important as small fruits for their high content of vitamins, bioactive organic substances with antibacterial and anticancer effects, anthocyanin pigments, and also as an excellent source of antioxidants. There is an increase in both interest and efforts to establish the production plantations of some of the cultivars of this species, suitable for cultivation as an alternative fruit crops, as medicinal plants and landscape ornamental ground cover plants. Large-scale production of high quality plants is needed for commercial plantation establishment. Traditional vegetative propagation of high bush blueberry by cuttings has not been successful due to poor rooting ability, considerable demand for large amount of mother plants, their limited seasonal growth and relatively high price. Generative reproduction does not produce homogeneous progeny.

ACS Style

M. G. Ostrolucká; A. Gajdošová; G. Libiaková; K. Hrubíková; M. Bežo. Protocol for Micropropagation of Selected Vaccinium spp. Protocols for Micropropagation of Woody Trees and Fruits 2007, 445 -455.

AMA Style

M. G. Ostrolucká, A. Gajdošová, G. Libiaková, K. Hrubíková, M. Bežo. Protocol for Micropropagation of Selected Vaccinium spp. Protocols for Micropropagation of Woody Trees and Fruits. 2007; ():445-455.

Chicago/Turabian Style

M. G. Ostrolucká; A. Gajdošová; G. Libiaková; K. Hrubíková; M. Bežo. 2007. "Protocol for Micropropagation of Selected Vaccinium spp." Protocols for Micropropagation of Woody Trees and Fruits , no. : 445-455.

Journal article
Published: 01 February 2000 in Journal of Experimental Botany
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Three types of callus tissues established from anther culture of eleven doubled haploid (DH) lines of wheat (Triticum aestivum L.) were evaluated for their ability in enhancing friable embryogenic (Type II) culture differentiation and genetic transformation. Differences between types of callus inocula were highly significant (P < 0.001), suggesting that the quality of the initial callus explant is of profound importance in encouraging the proliferation of Type II cultures. Other factors found to be crucial included weekly subculture of friable embryogenic callus tissues on a maintenance medium containing 30 microM dicamba and a predominance of amino-acid nitrogen supplement. Transfer and integration of the beta-glucuronidase gene was also affected by the type of inoculum when suitable embryogenic cell cultures were transformed using silicon carbide whiskers and high velocity microprojectiles. Expression of the hygromycin phosphotransferase selectable marker gene sequence was confirmed in all the stably transformed cell lines maintained on selection media containing lethal levels of hygromycin. Comparatively, there were differences in the frequency of regenerable, transgenic clonal segments between whisker-treated and microprojectile bombarded tissues mainly as a result of the fact that cultures vortexed with whiskers were more capable of post-treatment cell proliferation and embryo differentiation than those bombarded with cDNA-coated microprojectiles. Conditions for obtaining these results are outlined and discussed in relation to the suitability of the two transformation strategies for producing transgenic cell aggregates of wheat.

ACS Style

Ebiamadon Andi Brisibe; Alena Gajdosova; Annette Olesen; Sven Bode Andersen. Cytodifferentiation and transformation of embryogenic callus lines derived from anther culture of wheat. Journal of Experimental Botany 2000, 51, 187 -196.

AMA Style

Ebiamadon Andi Brisibe, Alena Gajdosova, Annette Olesen, Sven Bode Andersen. Cytodifferentiation and transformation of embryogenic callus lines derived from anther culture of wheat. Journal of Experimental Botany. 2000; 51 (343):187-196.

Chicago/Turabian Style

Ebiamadon Andi Brisibe; Alena Gajdosova; Annette Olesen; Sven Bode Andersen. 2000. "Cytodifferentiation and transformation of embryogenic callus lines derived from anther culture of wheat." Journal of Experimental Botany 51, no. 343: 187-196.