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The aim of this work was to assess the regeneration capacity of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica cv. ‘Jugana’ from different types of explants under various hormonal treatments. The whole leaves, petioles, and internodal segments of in vitro plants were examined as explants. Several plant growth regulators (cytokinins and auxins) were evaluated for their ability to induce adventitious regeneration. Direct and indirect organogenesis was achieved under certain culture conditions in both species. The frequency of shoot regeneration was strongly dependent on concentrations of plant growth regulators in the induction media (L.kamtschatica ‘Jugana’) or concentrations of plant growth regulators in the induction media and type of explant (A. alnifolia var. cusickii). Results showed that leaves were not suitable explants for A. alnifolia var. cusickii. Both species were able to regenerate shoots from internodal segments and petioles. The highest induction of shoots was obtained on Murashige and Skoog (MS) medium enriched with 2 mg/L thidiazuron (TDZ) and 0.5 mg/L indole-3-butyric acid (IBA) for Amelanchier alnifolia and with 1 mg/L TDZ and 0.2 mg/L indole-3-acetic acid (IAA) for L.kamtschatica ‘Jugana’. Obtained adventitious shoots were further proliferated in order to investigate their multiplication capacity. The multiplication of shoots was successful in all cultivars, with the best results reported in A. alnifolia var. cusickii (7.07 shoots/explant on average).
Júlia Hunková; Monika Szabóová; Alena Gajdošová. Protocols for Adventitious Regeneration of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica ‘Jugana’. Plants 2021, 10, 1155 .
AMA StyleJúlia Hunková, Monika Szabóová, Alena Gajdošová. Protocols for Adventitious Regeneration of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica ‘Jugana’. Plants. 2021; 10 (6):1155.
Chicago/Turabian StyleJúlia Hunková; Monika Szabóová; Alena Gajdošová. 2021. "Protocols for Adventitious Regeneration of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica ‘Jugana’." Plants 10, no. 6: 1155.
Grain amaranth is known as an alternative crop with exclusive nutritional value and health benefits. The purpose of this study was to investigate the effect of gamma irradiation on quantitative and qualitative amaranth seed traits, including 1000-seed weight, amino acids, fatty acids content, oil, and squalene yield. Two Slovak mutant varieties “Pribina” (A. cruentus) and “Zobor” (A.hypochondriacus x A. hybridus) were evaluated and compared to nonirradiated controls Ficha (A. cruentus L.) and K-433 (A. hypochondriacus x A. hybridus) and commercial varieties, Aztec (A. cruentus L.), Plainsman and Koniz (A. hypochondriacus x A. hybridus). Mutant varieties, “Pribina” and “Zobor”, showed superior 1000-seed weight performance compared to all investigated amaranth samples. The change in quantitative seed trait was accompanied by significantly higher oil and squalene content compared to commercial varieties. Moreover, significantly higher content of essential linoleic acid was detected in mutant variety “Zobor”. The present findings suggest that seeds of irradiation-derived varieties have high nutritional potential and can be used as a supplementary crop in the human diet.
Monika Szabóová; Michal Záhorský; Ján Gažo; Jeroen Geuens; Ann Vermoesen; Els D’Hondt; Andrea Hricová. Differences in Seed Weight, Amino Acid, Fatty Acid, Oil, and Squalene Content in γ-Irradiation-Developed and Commercial Amaranth Varieties (Amaranthus spp.). Plants 2020, 9, 1412 .
AMA StyleMonika Szabóová, Michal Záhorský, Ján Gažo, Jeroen Geuens, Ann Vermoesen, Els D’Hondt, Andrea Hricová. Differences in Seed Weight, Amino Acid, Fatty Acid, Oil, and Squalene Content in γ-Irradiation-Developed and Commercial Amaranth Varieties (Amaranthus spp.). Plants. 2020; 9 (11):1412.
Chicago/Turabian StyleMonika Szabóová; Michal Záhorský; Ján Gažo; Jeroen Geuens; Ann Vermoesen; Els D’Hondt; Andrea Hricová. 2020. "Differences in Seed Weight, Amino Acid, Fatty Acid, Oil, and Squalene Content in γ-Irradiation-Developed and Commercial Amaranth Varieties (Amaranthus spp.)." Plants 9, no. 11: 1412.
Berry fruit species are, in many countries, considered biologically and economically valuable and important species of small fruits. The aim of this work was to examine the influence of either decreased or increased mesos concentrations (MgSO4, CaCl2, and KH2PO4) on shoot multiplication of five cultivars of three small fruit species (Amelanchier alnifolia var. cusickii, Rubus fruticosus ‘Black Satin’ and ‘Loch Ness’, and Vaccinium corymbosum ‘Brigitta Blue’ and ‘Toro’). Mesos nutrients were manipulated from half to four times their base concentration. The results indicate that mesos manipulation significantly influences the number and length of shoots in most of the studied cultivars. The greatest multiplication rate for A. alnifolia was achieved with tripled mesos, whereas ‘Black Satin’ and ‘Loch Ness’ reacted positively to a lower (1–2x) concentration of mesos. Decreasing the concentration of mesos to half led to worse quality in both blackberry and Saskatoon shoots. ‘Brigitta Blue’ was more sensitive to greater mesos concentrations compared to ‘Toro’. Optimizing the mineral nutrition of plants cultivated in vitro enhances their multiplication rate and contributes to a higher production of good quality plantlets.
Júlia Hunková; Alena Gajdošová; Monika Szabóová. Effect of Mesos Components (MgSO4, CaCl2, KH2PO4) on In Vitro Shoot Growth of Blackberry, Blueberry, and Saskatoon. Plants 2020, 9, 935 .
AMA StyleJúlia Hunková, Alena Gajdošová, Monika Szabóová. Effect of Mesos Components (MgSO4, CaCl2, KH2PO4) on In Vitro Shoot Growth of Blackberry, Blueberry, and Saskatoon. Plants. 2020; 9 (8):935.
Chicago/Turabian StyleJúlia Hunková; Alena Gajdošová; Monika Szabóová. 2020. "Effect of Mesos Components (MgSO4, CaCl2, KH2PO4) on In Vitro Shoot Growth of Blackberry, Blueberry, and Saskatoon." Plants 9, no. 8: 935.
Andrea Hricová; Jozef Fejér; Gabriela Libiaková; Monika Szabová; Ján Gažo; Alena Gajdošová. Characterization of phenotypic and nutritional properties ofvaluable Amaranthus cruentus L. mutants. TURKISH JOURNAL OF AGRICULTURE AND FORESTRY 2016, 40, 761 -771.
AMA StyleAndrea Hricová, Jozef Fejér, Gabriela Libiaková, Monika Szabová, Ján Gažo, Alena Gajdošová. Characterization of phenotypic and nutritional properties ofvaluable Amaranthus cruentus L. mutants. TURKISH JOURNAL OF AGRICULTURE AND FORESTRY. 2016; 40 ():761-771.
Chicago/Turabian StyleAndrea Hricová; Jozef Fejér; Gabriela Libiaková; Monika Szabová; Ján Gažo; Alena Gajdošová. 2016. "Characterization of phenotypic and nutritional properties ofvaluable Amaranthus cruentus L. mutants." TURKISH JOURNAL OF AGRICULTURE AND FORESTRY 40, no. : 761-771.
Amaranth, a staple food source in ancient Aztec, Maya and Inca cultures, has been recognized as a 21st century crop. This superfood, known as Inca wheat, attracts the worldwide attention of researchers and farmers for its superior agronomical and technological properties but especially because of its exceptional nutritive value. A combination of two-dimensional electrophoresis (2-DE) with MS facilitating the effective differentiation of 13 classes of
Katarína Klubicová; Monika Szabová; Ludovit Skultety; Gabriela Libiaková; Andrea Hricová. Revealing the seed proteome of the health benefitting grain amaranth (Amaranthus cruentus L.). Chemical Papers 2016, 70, 1 .
AMA StyleKatarína Klubicová, Monika Szabová, Ludovit Skultety, Gabriela Libiaková, Andrea Hricová. Revealing the seed proteome of the health benefitting grain amaranth (Amaranthus cruentus L.). Chemical Papers. 2016; 70 (10):1.
Chicago/Turabian StyleKatarína Klubicová; Monika Szabová; Ludovit Skultety; Gabriela Libiaková; Andrea Hricová. 2016. "Revealing the seed proteome of the health benefitting grain amaranth (Amaranthus cruentus L.)." Chemical Papers 70, no. 10: 1.