Static stretching can increase the range of motion of a joint. Muscle-tendon unit stiffness (MTS) is potentially one of the main factors that influences the change in the range of motion after static stretching. However, to date, the effects of acute and long-term static stretching on MTS are not well understood. The purpose of this meta-analysis was to investigate the effects of acute and long-term static stretching training on MTS, in young healthy participants. PubMed, Web of Science, and EBSCO published before January 6, 2023, were searched and finally, 17 papers were included in the meta-analysis. Main meta-analysis was performed with a random-effect model and subgroup analyses, which included comparisons of sex (male vs. mixed sex and female) and muscle (hamstrings vs. plantar flexors) were also performed. Furthermore, a meta-regression was conducted to examine the effect of total stretching duration on MTS. For acute static stretching, the result of the meta-analysis showed a moderate decrease in MTS (effect size = -0.772, Z = -2.374, 95% confidence interval = -1.409 - -0.325, p = 0.018, I2 = 79.098). For long-term static stretching, there is no significant change in MTS (effect size = -0.608, Z = -1.761, 95% CI = -1.284 – 0.069, p = 0.078, I2 = 83.061). Subgroup analyses revealed no significant differences between sex (long-term, p = 0.209) or muscle (acute, p =0.295; long-term, p = 0.427). Moreover, there was a significant relationship between total stretching duration and MTS in acute static stretching (p = 0.011, R2 = 0.28), but not in long-term stretching (p = 0.085, R2 < 0.01). Whilst MTS decreased after acute static stretching, only a tendency of a decrease was seen after long-term stretching.

Takeuchi, K, Takemura, M, Nakamura, M, Tsukuda, F, and Miyakawa, S. Effects of active and passive warm-ups on range of motion, strength, and muscle passive properties in ankle plantarflexor muscles. J Strength Cond Res 35(1): 141–146, 2021—The purpose of this study was to examine the effects of active and passive warm-ups on flexibility and strength of calf muscles. Fourteen healthy males (age: 23.1 ± 2.6 years, height: 172.7 ± 5.6 cm, and body mass: 64.5 ± 7.0 kg) performed 3 types of warm-ups respectively for 10 minutes in a random order: an active warm-up by pedaling a cycling ergometer, an active warm-up doing repeated isometric contractions, and a passive warm-up in a hot water bath. To assess flexibility, range of motion (ROM) of ankle dorsiflexion, passive torque of ankle plantarflexion, and muscle tendon junction (MTJ) displacement were measured and then muscle tendon unit (MTU) stiffness was calculated. After the flexibility assessment, peak torque during maximum voluntary isometric contraction was measured to assess the isometric strength. These data were compared before and after each warm-up. As a result, all 3 types of warm-ups increased ROM (p < 0.05) and passive torque at terminal ROM (p < 0.01), but there were no significant changes in MTU stiffness or MTJ displacement. The active warm-up by pedaling a cycling ergometer increased peak torque during isometric contraction (p < 0.05), whereas the other warm-ups did not show significant alterations. In conclusion, the active warm-up with aerobic exercise increased flexibility and strength of the calf muscles.

The purpose of this study was to compare the duration of high-intensity static stretching on flexibility and strength in the hamstrings. Fourteen healthy males (20.8 ± 0.6 years, 170.7 ± 6.5 cm, 66.4 ± 9.9 kg) underwent high-intensity static stretching for three different durations (10, 15, and 20 seconds). The intensity of static stretching was set at the maximum point of discomfort. To examine the change in flexibility and strength, range of motion, peak passive torque, relative passive torque, muscle-tendon unit stiffness, peak torque of isokinetic knee flexion, and knee angle at peak torque of isokinetic knee flexion were measured. To evaluate a time course of pain, a numerical rating scale was described. Range of motion (P < 0.01), peak passive torque (P < 0.01), and knee angle at peak torque were increased at all interventions. Relative passive torque (P < 0.01) and muscle-tendon unit stiffness (P < 0.01) were decreased at all interventions. Peak torque decreased after 10 seconds of stretching (P < 0.05). Numerical rating scale during stretching was 8–9 levels in all interventions, the pain disappeared immediately after the post-measurements (median = 0). The results suggested that muscle-tendon unit stiffness decreased regardless of duration of high-intensity static stretching. However, peak torque of isokinetic knee flexion decreased after 10 seconds of high-intensity static stretching, though it was no change after for more than 15 seconds of stretching.

A previous study reported that 3-min of high-intensity static stretching at an intensity of 120% of range of motion (ROM) did not change the muscle stiffness of the rectus femoris, because of the overly high stress of the stretching. The purpose of this study was to examine the effects of high-intensity static stretching of a shorter duration or lower intensity on the flexibility of the rectus femoris than that of the previous study. Two experiments were conducted (Experiment 1 and 2). In Experiment 1, eleven healthy men underwent static stretching at the intensity of 120% of ROM for two different durations (1 and 3 min). In Experiment 2, fifteen healthy men underwent 3-min of static stretching at the intensity of 110% of ROM. The shear elastic modulus of the quadriceps were measured. In Experiment 1, ROM increased in both interventions (p < 0.01), but the shear elastic modulus of the rectus femoris was not changed. In Experiment 2, ROM significantly increased (p < 0.01), and the shear elastic modulus of the rectus femoris significantly decreased (p < 0.05). It was suggested that the stretching intensity (110%) is more important than stretching duration to decrease the muscle stiffness of the rectus femoris.