Prof. Pasquale Tripputi

University of Milan

Prof. Pasquale Tripputi

University of Milan

Co-authors

Following

Followers

Research Focus & Metrics

Explore full metrics on Scilit

Academic Career Length

42Years (1985-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

View All Publications

1 May 1986 in Proceedings of the National Academy of Sciences

Journal Article

Human histone genes map to multiple chromosomes.

Pasquale Tripputi

B S Emanuel

C M Croce

L G Green

G S Stein

J L Stein

Histone genes were mapped to at least three human chromosomes by Southern blot analysis of DNAs from a series of mouse-human somatic cell hybrids (using 32P-labeled cloned human histone DNA as probes). Chromosome assignment was confirmed by in situ hybridization of radiolabeled histone gene probes (3H-labeled) to metaphase chromosomes. One human histone gene cluster (lambda HHG41) containing an H3 and H4 gene resides only on chromosome 1, whereas other clusters containing core (H3, H4, H2A, and H2B) alone (lambda HHG17) or core together with H1 histone genes (lambda HHG415) have been assigned to chromosomes 1, 6, and 12. These results suggest that the multigene family of histone coding sequences that reside in a series of clusters may be derived from a single cluster containing one each of the genes for the five principal classes of histone proteins. During the course of evolution, a set of events, probably involving reduplication, sequence modification, and recombination, resulted in the present pattern of human histone gene distribution among several chromosomes.

2 September 1988 in Science

Journal Article

Two Mechanisms for the Extinction of Gene Expression in Hybrid Cells

Pasquale Tripputi

Sylvain Guerin

David D. Moore

When two different mammalian cell types are fused to generate a stable hybrid cell line, genes that are active in only one of the parents are frequently shut off, a phenomenon called extinction. In this study two distinct, complementary mechanisms for such extinction of growth hormone gene expression were identified. In hybrids formed by fusing fibroblasts to pituitary cells, pituitary-specific proteins that bind to the growth hormone promoter were absent. In addition, a negative regulatory element located near the rat growth hormone promoter was specifically activated.

1 July 1985 in Proceedings of the National Academy of Sciences

Journal Article

Human urokinase gene is located on the long arm of chromosome 10.

Pasquale Tripputi

F Blasi

P Verde

L A Cannizzaro

B S Emanuel

C M Croce

Urokinase is one of the two plasminogen activators that catalyze the conversion of inactive plasminogen to plasmin. By combining somatic cell genetics, in situ hybridization, and Southern hybridization, we localized the human urokinase gene on the distal third of the long arm (q24-qter) of chromosome 10.

1 April 2007 in Leukemia Research

Journal Article

HIC gene, a candidate suppressor gene within a minimal region of loss at 7q31.1 in myeloid neoplasms

Daniela Cigognini

Gianmarco Corneo

Elisa Fermo

Alberto Zanella

Pasquale Tripputi

1 December 1993 in AIDS Research and Human Retroviruses

Journal Article

Susceptibility of Human–Mouse T Cell Hybrids to HIV-Productive Infection

Dunia Ramarli

Caterina Cambiaggi

Carlo de Giuli Morghen

Pasquale Tripputi

Riccardo Ortolani

Roberto Accolla

Interspecies human x mouse cell hybrids were used to investigate the genetic basis of human permissivity to HTLV-III _B infection. T cell hybrids between the mouse BW 51.47 T lymphoma line and normal, PHA–IL-2 activated, human peripheral mononuclear cells (PBMCs) were generated. These hybrids preferentially segregated human chromosomes, as assessed by phenotype and karyotype analysis. Viral integration occurred only in those hybrids expressing CD4 ⁺ at the cell surface. However, infectious progeny production was demonstrated only in two of the three CD4 ⁺ hybrids tested. By segregation analysis, we could correlate the absence of human chromosomes 1, 3, and 9 with the lack of infectious viral progeny.

1 September 2001 in Leukemia Research

Journal Article

Chromosome 7 monosomy and deletions in myeloproliferative diseases

Pasquale Tripputi

Barbara Cassani

Rosamaria Alfano

Daniela Graziani

Daniela Cigognini

Guido Coggi

About Me

1978 M.D. University of Milan. 1978-81 Speciality in clinical and experimental hematology. 1982-85 post-doctoral fellow al Wistar Institute di Filadelfia, Pa, USA 1986-88 post-doctoral fellow al Massachusetts General Hospital , Dept of molecular biology and genetics Harvard University. Boston Ma, USA 1989-99 Assistant professor of Internal Medicine, Istituto di Scienze Biomediche San Gerardo, San Gerardo Hospital. Monza. University of Milan. 2000 Assistant professor of Internal Medicine, Dept. Medicine, Surgery and Dentistry, University of Milan. 2004 to present : Aggregate professor in Internal Medicine of the University of Milan. Main research interests : clinical and molecular medicine of therapy-related acule myeloid leukemia and myelodysplasia, chromosome 7 deletions and monosomy. deletion of chromosomal region 7q31.1 Prognostic factors in chronic lymphocytic leukemia. Linkage disequilibrium of a SNP in autoimmune diseases and between males and females. Expression and extinction of the rat  gene. Cloning and mapping of some human genes : Urokinase, Urokinase receptor , Tissue plasminogen activator; Histone genes; Oncogenes : Erb A, Erb B.

Prof. Pasquale Tripputi

Prof. Pasquale Tripputi

Research Focus & Metrics

Academic Career Length

Publication Type Breakdown

Representative Publications

About Me