In this work, we studied the impact of harvesting time on Fusarium mycotoxin occurrence in spring wheat and the effect of mycotoxin contamination on the quality of these grains. The spring wheat grains (Triticum aestivum L.) were collected in 2016–2018 when the crop had reached full maturity, 10 ± 2 days and 17 ± 3 days after full maturity. The grain samples were analyzed for Fusarium infection and co-contamination with mycotoxins deoxynivalenol (DON), zearalenone (ZEA), and T-2 toxin (T-2), as well as the quality of the wheat grains (mass per hectolitre, contents of protein, starch, ash and fat, particle size index (PSI), falling number, sedimentation, wet gluten content, and gluten index). The occurrence of Fusarium spp. fungi and the mycotoxins produced by them in the grains was mostly influenced by the harvesting time and meteorological conditions. The correlations between Fusarium species and the mycotoxins produced by them in the grains of spring wheat showed F. graminearum to be a dominant species, and as a result, higher concentrations of DON and ZEA were determined. The co-occurrence of all the three mycotoxins analyzed (deoxynivalenol, zearalenone, and T-2 toxin) was identified in wheat. In rainy years, a delay in harvesting resulted in diminished grain quality of spring wheat, as indicated by grain mass per hectolitre and falling number. Negative correlations were found in highly contaminated grains between mycotoxins (DON, ZEA, and T-2) and falling number and grain mass per hectolitre values.

Although Fusarium is mainly known as an agricultural pathogen that affects monocotyledonous plants, it can also infect different species of weeds in the agricultural environment, thereby contributing to the production of mycotoxins in cereals. In this study, we present new developmental data on the diversity of mycotoxins produced by Fusarium graminearum and Fusarium avenaceum strains from weeds under field conditions. Regarding the potential for the strain dependence of mycotoxin production, this study demonstrated that all F. graminearum strains isolated from weeds and spring wheat showed high potential for deoxynivalenol (DON), 3-acetyl-deoxynivalenol (3-ADON), 15-acetyl-deoxynivalenol (15-ADON), and nivalenol (NIV) production in spring wheat under field conditions. It was determined that F. graminearum is a typical producer of B-type trichothecenes. All strains of F. avenaceum isolated from spring wheat and weeds have the potential to produce enniatins and moniliformin in spring wheat. Each type of weed can host different Fusarium species and strains that produce completely different mycotoxins. Therefore, the distribution of mycotoxins in spring wheat grain may depend more on the Fusarium species or strains that infect the weeds than on the pathogen’s host plant species. The predominance of specific mycotoxins in cereals depends on the year’s weather conditions and the diversity of Fusarium species present in the field.

The common smut of corn, caused by Ustilago maydis, reduces the yield and quality of maize forage. When heavy infestations of corn smut occur, grain yields can be so severely decreased that the most viable economic alternative may be to harvest and ensile the crop. Only a couple of studies have attempted to investigate the influence of aerobic exposure on the nutritive value and aerobic stability of silage, which is prepared from smut-infected maize. In this study, individual whole corn plants were harvested by hand. The plants were distributed into three treatments: 0% infected, 50% infected, and 100% infected. The fresh forage was ensiled in triplicate for a 90-day period. Aerobic exposure lasted for 28 days. Samples were taken on the day of opening and on the 3rd, 7th, 14th, and 28th days. Near infrared spectroscopy (NIRS) calibration equations were used for the prediction of qualitative indicators. Silage prepared from 100% smut-infected maize had comparatively poor quality with dry matter loss, increasing pH and the low amount of starch. It was also distinguished with significant temperature increases from days 15 to 18 of aerobic exposure. Silage prepared from 50% smut-infected maize did not show significant quality changes over the period of the experiment, although it had inferior quality compared to the silage prepared from smut-free maize. While silage prepared from smut-infected maize had an overall worse quality than silage prepared from non-infected maize, it should not have an adverse effect on livestock health or production.

The aim of this study was to investigate whether, in the context of a higher incidence of Ustilago maydis and Fusarium spp. at optimal and delayed harvest times, a higher incidence of mycotoxin contamination in maize grains could be expected. The field experiment was carried out at the Lithuanian Research Centre for Agriculture and Forestry experimental fields over three consecutive years (2020–2022). Two maize hybrids (Duxxbury and Lapriora) with different FAO numbers were used. The experimental design in the field was a randomized complete block design. Harvesting took place at three different times: first at physiological maturity, and then 10 (±2) and 20 (±2) days after the first harvest. Each hybrid had four repetitions at different harvest times. The U. maydis infection was only detected in 2021 and after the first harvest cobs were further divided into four different groups with four repetitions: healthy cobs, cobs visually infected with Fusarium spp., cobs visually infected with common smut, and cobs visually infected with both pathogens. No U. maydis-damaged maize cobs were found in 2020 and 2022. The levels of Fusarium microscopic fungi in maize grains were also from 4 to 16 times higher in 2021 than in 2020 and 2022. Harvest delays in 2020 led to a significant deoxynivalenol concentration increase in the Duxxbury hybrid and an HT-2 concentration increase in the Lapriora hybrid. In 2021, deoxynivalenol, 3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol, and HT-2 concentrations significantly rose in both hybrids, but the T-2 concentration significantly increased only in the Lapriora hybrid. Deoxynivalenol concentrations were, respectively, 110 and 14.6 times higher than in cobs only infected with Fusarium spp. or U. maydis. Concentrations of 15-acetyl-deoxynivalenol were, respectively, 60, 67, and 43 times higher than in asymptomatic cobs and cobs only infected with Fusarium spp. or U. maydis. Cobs contaminated with both pathogens also had higher concentrations of 3-acetyl-deoxynivalenol. T-2 and HT-2 were detected in maize grains harvested from cobs infected only with Fusarium spp. The presence of U. maydis and Fusarium fungi in maize cobs, along with harvest delays, led to significant increases in mycotoxin concentrations, highlighting the importance of timely harvesting and pathogen management to mitigate mycotoxin contamination in maize grains.