Holocene environments have been reconstructed by multiproxy studies of an 850-cm-long core from Rio Curuá dating to >8000¹⁴C yr B.P. The low-energy river lies in the eastern Amazon rain forest in the Caxiuanã National Forest Reserve, 350 km west of Belem in northern Brazil. Sedimentological, mineralogical, and geochemical dates demonstrate that the deposits correspond to two different environments, sediments of an active river before 8000¹⁴C yr B.P. and later a passive river system. The pollen analytical results indicate four different local and regional Holocene paleoenvironmental periods: (1) a transition to a passive fluvial system and a well-drained terra firme (unflooded upland) Amazon rain forest with very limited development of inundated forests (várzea and igapó) (>7990–7030¹⁴C yr B.P.); (2) a sluggish river with a localMauritiapalm-swamp and similar regional vegetation, as before (7030–5970¹⁴C yr B.P.); (3) a passive river, forming shallow lake conditions and with still-abundant terra firme forest in the study region (5970–2470¹⁴C yr B.P.); and (4) a blocked river with high water levels and marked increase of inundated forests during the last 2470¹⁴C yr B.P. Increased charcoal during this last period suggests the first strong presence of humans in this region. The Atlantic sea level rise was probably the major factor in paleoenvironmental changes, but high water stands might also be due to greater annual rainfall during the late Holocene.

P. nigrum L. extracts and the piperine alkaloid have important antimicrobial, anti-inflammatory, and antioxidant properties. Therefore, in this study, we evaluated the antimicrobial activity and cytotoxicity of P. nigrum L. extracts and piperine, a compound isolated from the extracts of P. nigrum L. Extracts obtained via maceration, soxhlet, and purification steps, in addition to isolated piperine, were used in this study. Spectroscopic methods, such as nuclear magnetic resonance, scanning electron microscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry, were used to characterize piperine. In the microbiological analyses, the extract obtained via maceration-derived sample showed high efficiency in inhibiting Salmonella spp. (MIC < 100 μg/mL). The extract obtained via a soxhlet-derived sample showed promising inhibitory activity against almost all microorganisms, with negligible inhibition of Pseudomonas aeruginosa. Favorable inhibition coefficients were also observed against Staphylococcus aureus and Salmonella spp. (MIC < 100 μg/mL) for the extract obtained via purification of the steps-derived sample. Piperine showed an excellent inhibition coefficient against most microorganisms, with inactivity only observed against P. aeruginosa. Cytotoxicity evaluation assays in cancer cell lines revealed that piperine exhibited inhibitory potential on all tested tumor cell lines, causing a decrease in cell viability and achieving an IC₅₀ of less than 30 μg/mL. The analyzed extracts from P. nigrum L. seeds showed cytotoxic activity against tumor and non-tumor cell lines.

New pollen, micro-charcoal, sediment and mineral analyses of a radiocarbon-dated sediment core from the Serra Sul dos Carajás (southeast Amazonia) indicate changes between drier and wetter climatic conditions during the past 25,000 yr, reflected by fire events, expansion of savanna vegetation and no-analog Amazonian forest communities. A cool and dry last glacial maximum (LGM) and late glacial were followed by a wet phase in the early Holocene lasting for ca. 1200 yr, when tropical forest occurred under stable humid conditions. Subsequently, an increasingly warm, seasonal climate established. The onset of seasonality falls within the early Holocene warm period, with possibly longer dry seasons from 10,200 to 3400 cal yr BP, and an explicitly drier phase from 9000 to 3700 cal yr BP. Modern conditions with shorter dry seasons became established after 3400 cal yr BP. Taken together with paleoenvironmental evidence from elsewhere in the Amazon Basin, the observed changes in late Pleistocene and Holocene vegetation in the Serra Sul dos Carajás likely reflect large-scale shifts in precipitation patterns driven by the latitudinal displacement of the Inter-Tropical Convergence Zone and changes in sea-surface temperatures in the tropical Atlantic.