The importance of understanding spatial variability of soils is connected to crop management planning. This understanding makes it possible to treat soil not as a uniform, but a variable entity, and it enables site-specific management to increase production efficiency, which is the target of precision agriculture. Questions remain as the optimum soil sampling interval needed to make site-specific fertilizer recommendations in Brazil. The objectives of this study were: i) to evaluate the spatial variability of the main attributes that influence fertilization recommendations, using georeferenced soil samples arranged in grid patterns of different resolutions; ii) to compare the spatial maps generated with those obtained with the standard sampling of 1 sample ha-1, in order to verify the appropriateness of the spatial resolution. The attributes evaluated were phosphorus (P), potassium (K), organic matter (OM), base saturation (V%) and clay. Soil samples were collected in a 100 × 100 m georeferenced grid. Thinning was performed in order to create a grid with one sample every 2.07, 2.88, 3.75 and 7.20 ha. Geostatistical techniques, such as semivariogram and interpolation using kriging, were used to analyze the attributes at the different grid resolutions. This analysis was performed with the Vesper software package. The maps created by this method were compared using the kappa statistics. Additionally, correlation graphs were drawn by plotting the observed values against the estimated values using cross-validation. P, K and V%, a finer sampling resolution than the one using 1 sample ha-1 is required, while for OM and clay coarser resolutions of one sample every two and three hectares, respectively, may be acceptable.

We evaluated the use of airborne hyperspectral imaging and non-imaging sensors in the Vis—NIR—SWIR spectral region to assess particle size and soil organic matter in the surface layer of tropical soils (Oxisols, Ultisols, Entisols). The study area is near Piracicaba municipality, São Paulo state, Brazil, in a sugarcane cultivation area of 135 hectares. The study area, with bare soil, was imaged in April 2016 by the AisaFENIX aerotransported hyperspectral sensor, with spectral resolution of 3.5 nm between 380 and 970 nm, and 12 nm between 970 and 2500 nm. We collected 66 surface soil samples. The samples were analyzed for particle size and soil organic matter content. Laboratory spectral measurements were performed using a non-imaging spectroradiometer (ASD FieldSpec 3 Jr). Partial Least Square Regression (PLSR) was used to predict clay, silt, sand and soil organic matter (SOM). The PLSR functions developed were applied to the hyperspectral image of the study area, allowing development of a prediction map of clay, sand, and SOM. The developed PLSR models demonstrated the relationship between the predictor variables at the cross-validation step, both for the non-imaging and imaging sensors, when the highest r and R² values were obtained for clay, sand, and SOM, with R² over 0.67. We did not obtain a satisfactory model for silt content. For the non-imaging sensor at the prediction step, R² values for clay and SOM were over 0.7 and sand was lower than 0.54. The imaging sensor yielded models for clay, sand, and SOM with R² values of 0.62, 0.66, and 0.67, respectively. Pearson correlation between sensors was greater than 0.849 for the prediction of clay, sand, and SOM. Our study successfully generated, from the imaging sensor, a large-scale and detailed predicted soil maps for particle size and SOM, which are important in the management of tropical soils.

Soybean grain yield has regularly been impaired by drought periods, and the future climatic scenarios for soybean production might drastically impact yields worldwide. In this context, the knowledge of soybean yield is extremely important to subsidize government and corporative decisions over technical issues. This paper aimed to predict grain yield in soybean crop grown under different levels of water availability using reflectance spectroscopy and partial least square regression (PLSR). Field experiments were undertaken at Embrapa Soja (Brazilian Agricultural Research Corporation) in the 2016/2017, 2017/2018 and 2018/2019 cropping seasons. The data collected were analyzed following a split plot model in a randomized complete block design, with four blocks. The following water conditions were distributed in the field plots: irrigated (IRR), non-irrigated (NIRR) and water deficit induced at the vegetative (WDV) and reproductive stages (WDR) using rainout shelters. Soybean genotypes with different responses to water deficit were distributed in the subplots. Soil moisture and weather data were monitored daily. A total of 7216 leaf reflectance (from 400 to 2500 nm, measured by the FieldSpec 3 Jr spectroradiometer) was collected at 24 days in the three cropping seasons. The PLSR (p ≤ 0.05) was performed to predict soybean grain yield by its leaf-based reflectance spectroscopy. The results demonstrated the highest accuracy in soybean grain yield prediction at the R5 phenological stage, corresponding to the period when grains are being formed (R² ranging from 0.731 to 0.924 and the RMSE from 334 to 403 kg ha⁻¹—7.77 to 11.33%). Analyzing the three cropping seasons into a single PLSR model at R5 stage, R² equal to 0.775, 0.730 and 0.688 were obtained at the calibration, cross-validation and external validation stages, with RMSE lower than 634 kg ha⁻¹ (13.34%). The PLSR demonstrated higher accuracy in plants submitted to water deficit both at the vegetative and reproductive periods in comparison to plants under natural rainfall or irrigation.

Monitoring of soybean genotypes is important because of intellectual property over seed technology, better management over seed genetics, and more efficient strategies for its agricultural production process. This paper aims at spectrally classifying soybean genotypes submitted to diverse water availability levels at different phenological stages using leaf-based hyperspectral reflectance. Leaf reflectance spectra were collected using a hyperspectral proximal sensor. Two experiments were conducted as field trials: one experiment was at Embrapa Soja in the 2016/2017, 2017/2018, and 2018/2019 cropping seasons, where ten soybean genotypes were grown under four water conditions; and another experiment was in the experimental farm of Unoeste University in the 2018/2019 cropping season, where nine soybean genotypes were evaluated. The spectral data collected was divided into nine spectral datasets, comprising single and multiple cropping seasons (from 2016 to 2019), and two contrasting crop-growing environments. Principal component analysis, applied as an indicator of the explained variance of the reflectance spectra among genotypes within each spectral dataset, explained over 94% of the spectral variance in the first three principal components. Linear discriminant analysis, used to obtain a model of classification of each reflectance spectra of soybean leaves into each soybean genotype, achieved accuracy between 61% and 100% in the calibration procedure and between 50% and 100% in the validation procedure. Misclassification was observed only between genotypes from the same genetic background. The results demonstrated the great potential of the spectral classification of soybean genotypes at leaf-scale, regardless of the phenological stages or water status to which plants were submitted.

Reflectance hyperspectroscopy is recognised for its potential to elucidate biochemical changes, thereby enhancing the understanding of plant biochemistry. This study used the UV-VIS-NIR-SWIR spectral range to identify the different biochemical constituents in Hibiscus and Geranium plants. Hyperspectral vegetation indices (HVIs), principal component analysis (PCA), and correlation matrices provided in-depth insights into spectral differences. Through the application of advanced algorithms—such as PLS, VIP, iPLS-VIP, GA, RF, and CARS—the most responsive wavelengths were discerned. PLSR models consistently achieved R² values above 0.75, presenting noteworthy predictions of 0.86 for DPPH and 0.89 for lignin. The red-edge and SWIR bands displayed strong associations with pivotal plant pigments and structural molecules, thus expanding the perspectives on leaf spectral dynamics. These findings highlight the efficacy of spectroscopy coupled with multivariate analysis in evaluating the management of biochemical compounds. A technique was introduced to measure the photosynthetic pigments and structural compounds via hyperspectroscopy across UV-VIS-NIR-SWIR, underpinned by rapid multivariate PLSR. Collectively, our results underscore the burgeoning potential of hyperspectroscopy in precision agriculture. This indicates a promising paradigm shift in plant phenotyping and biochemical evaluation.