Márcio Moura-Alves

Dr. Márcio Moura-Alves

University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
orcid

Research Focus & Metrics

Academic Career Length

11Years (2016-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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7 June 2023 in Foods
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Journal Article
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Márcio Moura-AlvesMárcio Moura-Alves
Márcio Moura-Alves
AEAlexandra Sofia Esteves
Alexandra Sofia Esteves
MCMaria Ciríaco
Maria Ciríaco
JSJosé António Silva
José António Silva
Cristina Maria SaraivaCristina Maria Saraiva
Cristina Maria Saraiva
Meat deterioration during processing, distribution, and display can compromise the quality and safety of products, causing several undesirable changes and decreasing products’ shelf-life, which has a negative impact on the industry and consumers. In recent years, studies have been carried out using decontamination techniques and new packaging methodologies to overcome deterioration problems, increase sustainability, and reduce waste. Edible films and coatings obtained from biopolymers such as polysaccharides, proteins, and lipids, combined with active compounds, can be an alternative approach. This article focused on recent studies that used alternative biodegradable polymeric matrices in conjunction with natural compounds with antioxidant/antimicrobial activity on chicken meat. Its impact on physicochemical, microbiological, and sensory characteristics was evident, as well as the effect on its shelf-life. In general, different combinations of active edible films or coatings had a positive effect on the chicken meat. Different studies reported that the main results were a decrease in microbial growth and pathogen survival, a slowdown in lipid oxidation evolution, and an improvement in sensory quality and shelf-life (an increase from 4 to 12 days).

9 November 2023 in Foods
Verified
Journal Article
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Márcio Moura-AlvesMárcio Moura-Alves
Márcio Moura-Alves
Victor Gomes Lauriano SouzaVictor Gomes Lauriano Souza
Victor Gomes Lauriano Souza
JSJosé António Silva
José António Silva
AEAlexandra Sofia Esteves
Alexandra Sofia Esteves
Lorenzo  M. PastranaLorenzo M. Pastrana
Lorenzo M. Pastrana
Miguel CerqueiraMiguel Cerqueira
Miguel Cerqueira
Due to environmental concerns, there is an increasing need to reduce the use of synthetic and non-renewable packaging materials to reduce waste and increase sustainability. This study aimed to characterise sodium alginate edible-based films (SA) incorporated with laurel leaf extract (LLE) and olive leaf extract (OLE) obtained by ultrasound-assisted extraction. Determination of total phenolic content, antioxidant, and antimicrobial activity was performed for the extracts and films. Also, thickness, tensile strength, elongation at break, modulus of elasticity, opacity and colour, moisture content, water vapour permeability (WVP), Fourier-transform infrared spectroscopy (FTIR) spectra, and surface morphology by scanning electron microscope (SEM) analyses were performed for the films. LLE yielded better results in terms of phenolic content (195 mg GAE/g), antioxidant (2.1 TE/g extract) and antimicrobial activity (MIC at 1% for Listeria monocytogenes and Staphylococcus aureus, and 1.8% for Enterococcus faecalis). For the films, the simultaneous incorporation of LLE 1% (w/v) and OLE 1% (w/v) resulted in a significant reduction of approximately 2 log CFU/g against S. aureus. The addition of LLE and OLE extracts also proved to improve barrier properties (lower WVP for SA films with LLE 1% + OLE 1%, 3.49 × 10−11 g m−1 s−1 Pa−1) and promoted changes in resistance and flexibility. The results demonstrated that active alginate-based films can be valuable for enhancing food preservation.

1 January 2016 in Procedia Food Science
Verified
Journal Article
Open access
AGAna Rita Gouveia
Ana Rita Gouveia
Márcio Moura-AlvesMárcio Moura-Alves
Márcio Moura-Alves
JSJosé António Silva
José António Silva
Cristina Maria SaraivaCristina Maria Saraiva
Cristina Maria Saraiva
Sous vide cook-chill (SVCC) is a technology characterized by vacuum-packaging of raw or partially prepared foods before pasteurization, followed by rapid chilling and storage below 3°C. The application of essential oils (EOs) is a strategy to control pathogens and to extend the shelf life of products by reducing microbial levels and oxidative processes. The aim of this study was to evaluate the efficacy of Rosmarinus officinalis L. (rosemary) Thymus vulgaris L. (thyme) EOs against L. monocytogenes ATCC 679, inoculated in beef processed by SVCC stored at 2°C and 8°C for 1, 2, 3, 7 and 14 days. Leaves were dried and hydrodistilled in a Clevenger. Determination of minimum inhibitory concentration (MIC) assay was performed. Beef samples of m. longissimus thoracis et lumborum were packaged in bags inoculated and added individually with one of each EO at MIC values. Bags were vacuum-sealed, and samples were processed at 55°C/65min (for 3-log10 reduction). L. monocytogenes enumeration was done according to ISO 11290-2. A reduction of the population of L. monocytogenes was observed in all samples at 2°C. At day 14, the population of L. monocytogenes was similar in thyme and control at 2°C and 8°C. Inversely, rosemary at both temperatures show an added reduction of about 2-log10, comparatively to control. These results support the possibility of using rosemary as natural preservative to contribute in the reduction of L. monocytogenes and confirm the importance of using adequate chilling storage for maintain this pathogen at acceptable levels in view to prevent the risk for consumers

30 September 2021 in Foods
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Journal Article
Open access
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CACristina Aires
Cristina Aires
Cristina Maria SaraivaCristina Maria Saraiva
Cristina Maria Saraiva
MFMaria da Conceição Castro Medeiros de Castro Fontes
Maria da Conceição Castro Medeiros de Castro Fontes
DMDaniel Moreira
Daniel Moreira
Márcio Moura-AlvesMárcio Moura-Alves
Márcio Moura-Alves
Carla GonçalvesCarla Gonçalves
Carla Gonçalves
Background: This study aims to evaluate food waste and menu quality in two canteens (A and B) from a Portuguese public university in order to identify challenges and opportunities to improve the food service. Methods: Food waste included the analysis of two canteens over 5 consecutive days by selective aggregate weighing. A qualitative evaluation of a 5-week menu cycle related to lunches was performed through the Qualitative Evaluation of Menus (AQE-d) method. Results: Both menus have “satisfactory” evaluations and lower adequacy to the dietary guidelines in criteria A, which evaluates general items from the dish, and in criteria B, which evaluates meat, fish and eggs. The calculated mean of food waste in both canteens exceeded the acceptable limit of 10%, except for the vegetarian (7.5%) dish in canteen A. The biggest waste was found in the vegetarian dish (16.8%) in canteen A. In meat dishes the conduit presents more waste (17.0%) than in fish and vegetarian dishes. Among these, the vegetables were the most wasted (25.3% and 27.9%, respectively). Conclusion: This work presents some insights to future interventions in the direction of a healthier and more sustainable foodservice.

1 October 2020 in LWT
Verified
Journal Article
Closed access
Márcio Moura-AlvesMárcio Moura-Alves
Márcio Moura-Alves
AGAna R. Gouveia
Ana R. Gouveia
Jose Manuel M. M. de AlmeidaJose Manuel M. M. de Almeida
Jose Manuel M. M. de Almeida
FMFilipe Monteiro-Silva
Filipe Monteiro-Silva
FMFilipe Monteiro-Silva
Filipe Monteiro-Silva
Cristina Maria SaraivaCristina Maria Saraiva
Cristina Maria Saraiva

24 February 2022 in Journal of Food Protection
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Journal Article
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Márcio Moura-AlvesMárcio Moura-Alves
Márcio Moura-Alves
MCMarta Carvalho
Marta Carvalho
DRDeise Helena Baggio Ribeiro
Deise Helena Baggio Ribeiro
JBJoana Barbosa
Joana Barbosa
LSLeonor Silveira
Leonor Silveira
AEAlexandra Esteves
Alexandra Esteves
The monitoring of carcass surface contamination along the slaughter line enables verification of slaughter operation hygiene and the use of good manufacturing practices. Pork meat is a common source of human nontyphoidal salmonellosis, one of the most frequently reported foodborne illnesses worldwide. This study was conducted to gather data on microbial loads before and after evisceration on the surfaces of swine carcasses in two slaughterhouses. The presence of Salmonella enterica was evaluated only after evisceration on carcass surfaces and in livers and floor drains (environmental samples) because pigs are common carriers of this pathogen. The contamination of carcass surfaces was evaluated by delimitation of surface area with sterilized templates (100 cm2), and surface samples were collected with gauze swabs. Total aerobic mesophilic bacteria, Enterobacteriaceae, and Escherichia coli were enumerated. Significant differences (P < 0.05) in counts of mesophilic bacteria, Enterobacteriaceae, and E. coli were found on the external carcass surfaces, with higher counts after evisceration. The neck and abdominal areas had higher levels of mesophilic bacteria, Enterobacteriaceae, and E. coli and a high prevalence of Salmonella. Salmonella was detected in only one of the studied slaughterhouses; 19 (7.3%) of 259 analyzed carcass samples were positive for Salmonella, and Salmonella was detected in two livers and two floor drains. A total of 52 Salmonella isolates (44 from carcasses, 5 from livers, and 3 from drains) were recovered. Three Salmonella serovars (Typhimurium 4,5:i− , Wernigerone, and Derby) were identified, and 53.8% of the 52 isolates were multidrug resistant. The results reveal the need for continuous improvement of slaughtering operations and implementation of good manufacturing practices to ensure the safety of pork produced in Portugal.HIGHLIGHTSHigher counts of indicator microorganisms were obtained after swine evisceration.Salmonella was detected in 7.3% of swine carcass surface samples.Three Salmonella serovars were identified: Typhimurium 4,5:i− , Wernigerone, and Derby.Of the 52 Salmonella isolates, 53.8% were multidrug resistant.