The objective of the present work was to evaluate novel active films made with biopolymeric matrices as carriers of a living Bdellovibrio bacteriovorus HD100 strain, a predatory bacterium with antimicrobial potentials against pathogens. Biopolymer films were prepared by a casting method using the following mixtures: collagen/sodium alginate/sorbitol (CA-S), collagen/sodium alginate/glycerol (CA-G), and tapioca starch/sodium alginate/glycerol (StA-G). The effects of the film formulations on the viability of the B. bacteriovorus was investigated by using Fourier Transform Infrared (FTIR) spectroscopy, Differential Scanning Calorimetry (DSC), and Scanning Electron Microscopy (SEM). SEM showed that Bdellovibrio bacteriovorus morphology was not altered in the polymeric films. FTIR spectroscopy provided information about the structural composition of the films. CA-S showed less reduction in the viability of B. bacteriovorus after its entrapment; thus, CA-S proved to be a better agent for the immobilization and preservation of B. bacteriovorus to enhance its predatory activities during application against Escherichia coli.

Diabetic wounds infected with multidrug-resistant bacteria represent a major clinical challenge due to delayed healing and limited therapeutic options. In this study, we evaluated the therapeutic efficacy and biosafety of a biopolymeric skin patch loaded with the predatory bacterium Bdellovibrio bacteriovorus HD100 in a murine model of diabetic wounds infected with Pseudomonas aeruginosa. In vitro assays demonstrated that B. bacteriovorus HD100 reduced P. aeruginosa populations by approximately 3 log units within 48 h. In vivo, diabetic mice treated with the B. bacteriovorus-loaded patch achieved complete wound closure within $12\pm 1$ days, compared with $16\pm 1$ days in mice treated with conventional antibiotic therapy (piperacillin/tazobactam, 16 mg/kg; single dose). Non-diabetic mice treated with biopolymeric patches, with or without the predatory bacterium, exhibited complete wound closure within 9–10 days. Molecular analysis by PCR revealed no detectable dissemination of B. bacteriovorus DNA to internal organs (liver, spleen, kidney, or brain), indicating the systemic biosafety of topical application. Overall, these results demonstrate that B. bacteriovorus-based skin patches significantly accelerate wound closure in infected diabetic wounds and represent a promising localized biological alternative to conventional antibiotic therapy.

Urinary tract infections (UTIs) are among the most common bacterial infections worldwide, accounting for approximately 150 million cases annually and an estimated 3.8 million cases each year in Mexico. The principal uropathogens are Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis, all of which are opportunistic zoonotic bacteria associated with humans, companion animals, livestock, and wildlife. Within the One Health framework, these pathogens and their antimicrobial resistance (AMR) determinants may circulate across healthcare settings, communities, animals, and the environment, highlighting the need for integrated surveillance. Although conventional urine culture remains the diagnostic gold standard, rapid molecular methods can facilitate timely diagnosis and support antimicrobial stewardship. This study analyzed 300 urine samples collected from three hospitals representing different levels of healthcare complexity in Reynosa, Tamaulipas, Mexico. Conventional urine culture and species-specific PCR assays targeting E. coli, K. pneumoniae, and P. mirabilis were performed. Fifty-six samples yielded positive urine cultures, with E. coli identified as the predominant uropathogen (85.7%, 48/56), followed by K. pneumoniae (10.7%, 6/56) and P. mirabilis (3.6%, 2/56). Positive cases were more frequent among females (70%). In addition, a multiplex PCR assay was developed and validated for the simultaneous detection of the three pathogens. The assay was also successfully applied directly to unprocessed urine samples, provided that urine represented no more than 20% of the total PCR reaction volume. The multiplex PCR demonstrated 100% concordance with conventional culture results while substantially reducing diagnostic turnaround time. To our knowledge, this is the first study conducted in Reynosa, Tamaulipas, to integrate conventional microbiological and molecular diagnostics for UTIs within a One Health framework. These findings establish a methodological baseline for regional surveillance of uropathogens at the human–healthcare–environment interface, supporting future cross-border One Health surveillance initiatives.