Avocado oil is a very valuable agro-industrial product which can be perishable in a short time if it is not stored in the right conditions. The encapsulation of the oils through the spray drying technique protects them from oxidation and facilitates their incorporation into different pharmaceutical products and food matrices; however, the selection of environmentally friendly emulsifiers is a great challenge. Four formulations of the following solid particles: Gum Arabic, HI-CAP^®100 starch, and phosphorylated waxy maize starch, were selected to prepare avocado oil Pickering emulsions. Two of the formulations have the same composition, but one of them was emulsified by rotor-stator homogenization. The rest of the emulsions were emulsified by combining rotor-stator plus ultrasound methods. The protective effect of mixed particle emulsifiers in avocado oil encapsulated by spray drying was based on the efficiency of encapsulation. The best results were achieved when avocado oil was emulsified with a mixture of phosphorylated starch/HI-CAP^®100, where it presented the highest encapsulation efficiency.

Acanthocereus tetragonus (L.) Hummelinck is used as an alternative food source in some Mexican communities. It has been shown that the young stems of A. tetragonus provide crude protein, fiber, and essential minerals for humans. In this work, we analyzed the phytochemical profile, the total phenolic content (TPC), and the antioxidant activity of cooked and crude samples of A. tetragonus to assess its functional metabolite contribution to humans. The phytochemical profile was analyzed using Ultra-High-Performance Liquid Chromatography coupled to High-Resolution Mass Spectrometry (UHPLC-PDA-HESI-Orbitrap-MS/MS). Under the proposed conditions, 35 metabolites were separated and tentatively identified. Of the separated metabolites, 16 occurred exclusively in cooked samples, 6 in crude samples, and 9 in both crude and cooked samples. Among the detected compounds, carboxylic acids, such as threonic, citric, and malic acids, phenolic acids, and glycosylated flavonoids (luteolin-O-rutinoside) were detected. The TPC and antioxidant activity were analyzed using the Folin–Ciocalteu method and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical inhibition method, respectively. The TPC and antioxidant activity were significantly reduced in the cooked samples. We found that some metabolites remained intact after the cooking process, suggesting that A. tetragonus represents a source of functional metabolites for people who consume this plant species.

This study evaluated the potential of a biocoagulant produced from prickly pear peel waste valorization and its use as a biocoagulant aid mixed with aluminum sulfate to remove turbidity in domestic wastewater. A central composite design (CCD) and a simplex lattice design (SLD) of two components (biocoagulant and aluminum sulfate) were developed to determine the optimal doses and pH of the biocoagulant and optimal mixing proportions. Both designs optimized the coagulation process from an analysis of variance to fit the experimental data to mathematical models and an optimization analysis to obtain the highest percentage of turbidity removal. The results showed that a water pH of 4 and a biocoagulant dose of 100 mg/L are optimal conditions for a turbidity removal of 76.1%. The potential decreases to 51.7% when the wastewater pH is maintained at 7.8 and a dose of 250 mg/L is used. This efficiency could be increased to 58.2% by using a mixture with optimal proportions of 30% biocoagulant and 70% aluminum sulfate. The experimental data were fitted to two quadratic models, estimating model prediction errors of 0.42% and 2.34%, respectively. Therefore, these results support the valorization of prickly pear peel waste to produce a biocoagulant, which could be used in acid and alkaline wastewater or as a biocoagulant aid mixed with aluminum sulfate.

Diabetic wounds infected with multidrug-resistant bacteria represent a major clinical challenge due to delayed healing and limited therapeutic options. In this study, we evaluated the therapeutic efficacy and biosafety of a biopolymeric skin patch loaded with the predatory bacterium Bdellovibrio bacteriovorus HD100 in a murine model of diabetic wounds infected with Pseudomonas aeruginosa. In vitro assays demonstrated that B. bacteriovorus HD100 reduced P. aeruginosa populations by approximately 3 log units within 48 h. In vivo, diabetic mice treated with the B. bacteriovorus-loaded patch achieved complete wound closure within $12\pm 1$ days, compared with $16\pm 1$ days in mice treated with conventional antibiotic therapy (piperacillin/tazobactam, 16 mg/kg; single dose). Non-diabetic mice treated with biopolymeric patches, with or without the predatory bacterium, exhibited complete wound closure within 9–10 days. Molecular analysis by PCR revealed no detectable dissemination of B. bacteriovorus DNA to internal organs (liver, spleen, kidney, or brain), indicating the systemic biosafety of topical application. Overall, these results demonstrate that B. bacteriovorus-based skin patches significantly accelerate wound closure in infected diabetic wounds and represent a promising localized biological alternative to conventional antibiotic therapy.